pycom06g21620

UPF0426 protein At1g28150

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0014155
Physical Location & Seq
Forward (+)
24196332 .. 24197757
1426 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 513 bp
ATGAAGCGAAACGGCCTTAAAATAGAGAGTCTGAGAGAGAGAGAGGGGCAAACATCTACCGGTGGGGAATACGAACCAGAACCATCAAACACGAGAAGAGAGCACCGGGAGTTCAAGTTAGTCAACATGTCTGTGTTTATTGTGAATTCTCCTTCGTGTGCACTGTGGAAATCGAAGAAGCCTGGCCCTGGGTTTATTAAACCACCGACATCACCGAAGCAGCAGGTTCCCAGAAGCAGCAGCCGCAGAAAATTGTGTTTGGGGAACGGCAATGGGATTAAGGCGTTTTTCTTCAATCCCAACGAAGAACCCATCCTCAGAGAAGCGCTTAAGGAGCCAGTTGCCTTCATGGGAGGGATGTTCGCTGGTCTTTTAAGGCTTGATTTGAATGAAGATCCACTCAAGGATTGGGTTTCAAGGACTGTGGAAGCCACTGGAATCACCGAGGAAGAAATTGTTGCTGACGGGTTAAAACCAGCGGGAGAAGCCCCGCTACAGATTGAGATTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0010287 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

171

Amino Acids

18.94

Weight (kDa)

7.74

Isoelectric Point (pI)

64.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UPF0426 PF26369 108 - 142 1.7e-18 UPF0426 family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014155)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28150 AT1G28150
fragaria_vesca FvH4_5g18600
malus_domestica MD06G1241100.v1.1 MD14G1247300.v1.1
prunus_persica Prupe.5G245400_v2.0.a1
pyrus_communis pycom06g21620
rosa_chinensis RchiOBHm_Chr7g0176901
rosa_laevigata RLG00000005574
rosa_multiflora Rmu_sc0005310.1_g000045
rosa_roxburghii Rroxscaffold_3G00276300
rosa_rugosa Rorug06G0401700
rosa_samantha Rh7AG001200 Rh7BG001600 Rh7CG001000 Rh7DG001300
rosa_wichuraiana Rw7G000090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 214
AciI CCGC 3 cut(s) 244, 479, 491
AclWI GGATC 1 cut(s) 389
AcsI RAATTY 1 cut(s) 145
AfeI AGCGCT 1 cut(s) 327
AfiI CCNNNNNNNGG 1 cut(s) 188
AflII CTTAAG 1 cut(s) 329
AflIII ACRYGT 1 cut(s) 126
AgeI ACCGGT 1 cut(s) 59
AgsI TTSAA 5 cut(s) 115, 295, 388, 417, 509
AjnI CCWGG 2 cut(s) 181, 187
Alw21I GWGCWC 2 cut(s) 105, 163
Alw44I GTGCAC 1 cut(s) 159
AlwI GGATC 1 cut(s) 389
Aor51HI AGCGCT 1 cut(s) 327
AoxI GGCC 2 cut(s) 13, 184
ApaLI GTGCAC 1 cut(s) 159
ApeKI GCWGC 3 cut(s) 220, 237, 240
ApoI RAATTY 1 cut(s) 145
AsiGI ACCGGT 1 cut(s) 59
AspLEI GCGC 1 cut(s) 328
AspS9I GGNCC 1 cut(s) 185
AsuC2I CCSGG 1 cut(s) 107
AsuHPI GGTGA 2 cut(s) 204, 433
BaeGI GKGCMC 1 cut(s) 163
BarI GAAGNNNNNNTAC 2 cut(s) 477, 509
BauI CACGAG 1 cut(s) 91
Bbv12I GWGCWC 2 cut(s) 105, 163
BbvI GCAGC 3 cut(s) 232, 249, 252
BccI CCATC 2 cut(s) 91, 320
BceAI ACGGC 2 cut(s) 28, 283
BciT130I CCWGG 2 cut(s) 183, 189
BcnI CCSGG 1 cut(s) 107
BfmI CTRYAG 1 cut(s) 494
BfoI RGCGCY 1 cut(s) 329
BfrI CTTAAG 1 cut(s) 329
BfuAI ACCTGC 1 cut(s) 214
BisI GCNGC 4 cut(s) 221, 238, 241, 244
BlsI GCNGC 4 cut(s) 222, 239, 242, 245
Bme1390I CCNGG 3 cut(s) 107, 183, 189
BmgT120I GGNCC 1 cut(s) 185
BmiI GGNNCC 2 cut(s) 228, 336
BmrFI CCNGG 3 cut(s) 107, 183, 189
BpuEI CTTGAG 1 cut(s) 386
BpuMI CCSGG 1 cut(s) 107
BsaJI CCNNGG 3 cut(s) 187, 188, 444
BsaWI WCCGGW 1 cut(s) 59
BsaXI ACNNNNNCTCC 2 cut(s) 101, 131
Bsc4I CCNNNNNNNGG 1 cut(s) 188
Bse118I RCCGGY 1 cut(s) 59
Bse1I ACTGG 2 cut(s) 338, 439
Bse3DI GCAATG 1 cut(s) 277
BseBI CCWGG 2 cut(s) 183, 189
BseDI CCNNGG 3 cut(s) 187, 188, 444
BseGI GGATG 2 cut(s) 312, 363
BseLI CCNNNNNNNGG 1 cut(s) 188
BseMI GCAATG 1 cut(s) 277
BseMII CTCAG 2 cut(s) 23, 331
BseNI ACTGG 2 cut(s) 338, 439
BseSI GKGCMC 1 cut(s) 163
BseXI GCAGC 3 cut(s) 232, 249, 252
BshFI GGCC 2 cut(s) 15, 186
BshTI ACCGGT 1 cut(s) 59
BsiHKAI GWGCWC 2 cut(s) 105, 163
BsiSI CCGG 2 cut(s) 60, 106
BslI CCNNNNNNNGG 1 cut(s) 188
BsnI GGCC 2 cut(s) 15, 186
Bsp1286I GDGCHC 2 cut(s) 105, 163
Bsp143I GATC 1 cut(s) 394
BspACI CCGC 3 cut(s) 244, 479, 491
BspANI GGCC 2 cut(s) 15, 186
BspCNI CTCAG 2 cut(s) 24, 330
BspLI GGNNCC 2 cut(s) 228, 336
BspMI ACCTGC 1 cut(s) 214
BspPI GGATC 1 cut(s) 389
BspTI CTTAAG 1 cut(s) 329
BsrDI GCAATG 1 cut(s) 277
BsrFI RCCGGY 1 cut(s) 59
BsrI ACTGG 2 cut(s) 338, 439
BssAI RCCGGY 1 cut(s) 59
BssECI CCNNGG 3 cut(s) 187, 188, 444
BssMI GATC 1 cut(s) 394
BssSI CACGAG 1 cut(s) 91
Bst2BI CACGAG 1 cut(s) 91
Bst2UI CCWGG 2 cut(s) 183, 189
Bst4CI ACNGT 2 cut(s) 165, 424
Bst6I CTCTTC 1 cut(s) 91
BstAFI CTTAAG 1 cut(s) 329
BstDEI CTNAG 2 cut(s) 32, 317
BstF5I GGATG 2 cut(s) 312, 363
BstH2I RGCGCY 1 cut(s) 329
BstHHI GCGC 1 cut(s) 328
BstKTI GATC 1 cut(s) 397
BstMBI GATC 1 cut(s) 394
BstMWI GCNNNNNNNGC 3 cut(s) 243, 334, 485
BstNI CCWGG 2 cut(s) 183, 189
BstNSI RCATGY 1 cut(s) 130
BstSCI CCNGG 3 cut(s) 105, 181, 187
BstSFI CTRYAG 1 cut(s) 494
BstSLI GKGCMC 1 cut(s) 163
BstV1I GCAGC 3 cut(s) 232, 249, 252
BstX2I RGATCY 1 cut(s) 394
BstYI RGATCY 1 cut(s) 394
BsuRI GGCC 2 cut(s) 15, 186
BtsCI GGATG 2 cut(s) 312, 363
BtsIMutI CAGTG 2 cut(s) 161, 432
BveI ACCTGC 1 cut(s) 214
CfoI GCGC 1 cut(s) 328
Cfr10I RCCGGY 1 cut(s) 59
Cfr13I GGNCC 1 cut(s) 185
CspAI ACCGGT 1 cut(s) 59
CspCI CAANNNNNGTGG 2 cut(s) 405, 440
CviAII CATG 2 cut(s) 127, 349
CviJI RGCY 8 cut(s) 15, 181, 186, 243, 337, 379, 431, 488
CviKI_1 RGCY 8 cut(s) 15, 181, 186, 243, 337, 379, 431, 488
DdeI CTNAG 2 cut(s) 32, 317
DpnI GATC 1 cut(s) 396
DpnII GATC 1 cut(s) 394
Eam1104I CTCTTC 1 cut(s) 91
EarI CTCTTC 1 cut(s) 91
Eco47III AGCGCT 1 cut(s) 327
EcoRI GAATTC 1 cut(s) 145
EcoRII CCWGG 2 cut(s) 181, 187
FaeI CATG 2 cut(s) 130, 352
FaiI YATR 2 cut(s) 128, 350
FatI CATG 2 cut(s) 126, 348
FauI CCCGC 2 cut(s) 472, 498
Fnu4HI GCNGC 4 cut(s) 221, 238, 241, 244
FokI GGATG 2 cut(s) 299, 370
Fsp4HI GCNGC 4 cut(s) 221, 238, 241, 244
GlaI GCGC 1 cut(s) 327
GluI GCNGC 4 cut(s) 221, 238, 241, 244
HaeII RGCGCY 1 cut(s) 329
HaeIII GGCC 2 cut(s) 15, 186
HapII CCGG 2 cut(s) 60, 106
HhaI GCGC 1 cut(s) 328
Hin1II CATG 2 cut(s) 130, 352
Hin6I GCGC 1 cut(s) 326
HinP1I GCGC 1 cut(s) 326
HincII GTYRAC 1 cut(s) 124
HindII GTYRAC 1 cut(s) 124
HinfI GANTC 2 cut(s) 28, 438
HpaII CCGG 2 cut(s) 60, 106
HphI GGTGA 2 cut(s) 204, 433
Hpy166II GTNNAC 2 cut(s) 124, 161
Hpy188I TCNGA 2 cut(s) 33, 320
Hpy8I GTNNAC 2 cut(s) 124, 161
HpyAV CCTTC 2 cut(s) 162, 355
HpyCH4III ACNGT 2 cut(s) 165, 424
HpyCH4V TGCA 1 cut(s) 161
HpyF10VI GCNNNNNNNGC 3 cut(s) 243, 334, 485
HpyF3I CTNAG 2 cut(s) 32, 317
Hsp92II CATG 2 cut(s) 130, 352
HspAI GCGC 1 cut(s) 326
Kzo9I GATC 1 cut(s) 394
LmnI GCTCC 1 cut(s) 334
Lsp1109I GCAGC 3 cut(s) 232, 249, 252
MalI GATC 1 cut(s) 396
MboI GATC 1 cut(s) 394
MboII GAAGA 6 cut(s) 108, 187, 283, 317, 404, 461
MflI RGATCY 1 cut(s) 394
MhlI GDGCHC 2 cut(s) 105, 163
MluCI AATT 3 cut(s) 145, 251, 453
MlyI GAGTC 1 cut(s) 37
MnlI CCTC 4 cut(s) 37, 326, 347, 439
MseI TTAA 6 cut(s) 18, 198, 279, 330, 374, 470
MslI CAYNNNNRTG 1 cut(s) 131
MspA1I CMGCKG 1 cut(s) 479
MspCI CTTAAG 1 cut(s) 329
MspI CCGG 2 cut(s) 60, 106
MspR9I CCNGG 3 cut(s) 107, 183, 189
MvaI CCWGG 2 cut(s) 183, 189
MwoI GCNNNNNNNGC 3 cut(s) 243, 334, 485
NciI CCSGG 1 cut(s) 107
NdeII GATC 1 cut(s) 394
NlaIII CATG 2 cut(s) 130, 352
NlaIV GGNNCC 2 cut(s) 228, 336
NspI RCATGY 1 cut(s) 130
PasI CCCWGGG 1 cut(s) 188
PciI ACATGT 1 cut(s) 126
PfeI GAWTC 1 cut(s) 438
PinAI ACCGGT 1 cut(s) 59
PkrI GCNGC 4 cut(s) 222, 239, 242, 245
PleI GAGTC 1 cut(s) 36
PpsI GAGTC 1 cut(s) 36
PscI ACATGT 1 cut(s) 126
Psp6I CCWGG 2 cut(s) 181, 187
PspGI CCWGG 2 cut(s) 181, 187
PspN4I GGNNCC 2 cut(s) 228, 336
PspPI GGNCC 1 cut(s) 185
PsuI RGATCY 1 cut(s) 394
RseI CAYNNNNRTG 1 cut(s) 131
SaqAI TTAA 6 cut(s) 18, 198, 279, 330, 374, 470
SatI GCNGC 4 cut(s) 221, 238, 241, 244
Sau3AI GATC 1 cut(s) 394
Sau96I GGNCC 1 cut(s) 185
SchI GAGTC 1 cut(s) 37
ScrFI CCNGG 3 cut(s) 107, 183, 189
SduI GDGCHC 2 cut(s) 105, 163
SetI ASST 1 cut(s) 228
SfcI CTRYAG 1 cut(s) 494
SmiMI CAYNNNNRTG 1 cut(s) 131
SmlI CTYRAG 2 cut(s) 329, 401
SmoI CTYRAG 2 cut(s) 329, 401
Sse9I AATT 3 cut(s) 145, 251, 453
SsiI CCGC 3 cut(s) 244, 479, 491
StyD4I CCNGG 3 cut(s) 105, 181, 187
TaaI ACNGT 2 cut(s) 165, 424
TaqI TCGA 1 cut(s) 173
TasI AATT 3 cut(s) 145, 251, 453
TauI GCSGC 1 cut(s) 246
TfiI GAWTC 1 cut(s) 438
Tru1I TTAA 6 cut(s) 18, 198, 279, 330, 374, 470
Tru9I TTAA 6 cut(s) 18, 198, 279, 330, 374, 470
TscAI CASTG 2 cut(s) 168, 439
TseI GCWGC 3 cut(s) 220, 237, 240
TspDTI ATGAA 3 cut(s) 17, 337, 405
TspRI CASTG 2 cut(s) 168, 439
Vha464I CTTAAG 1 cut(s) 329
VneI GTGCAC 1 cut(s) 159
XapI RAATTY 1 cut(s) 145
XceI RCATGY 1 cut(s) 130
XcmI CCANNNNNNNNNTGG 1 cut(s) 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.