pycom07g09320

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0003149
Physical Location & Seq
Forward (+)
8578999 .. 8579641
643 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 441 bp
ATGAATTTTGCAACATCAATATGCAGTAGGTTGGGTCTGAAGAACATTGTTACGAATACTCCCGTATACAGCAGTGCTTCTGGTACGAATGTCACGGCAGAAGGATTGAGTTTGACCTTTAGGCGCTGGGCTACTAAAAAATCTGCTGGATCCACCAAAAATGGTCGAGATTCAAATCCCAAATTTCTTGGAGTGAAGAAATTTGGCGGAGAGAGAGTGATACCTGGAAACATCATTGTTCGCCAAAGAGGCACCCGTTTTCATCCTGGAAATTATGTTGGTTTAGGGAAGGATCACACCCTTTTTGCTCTGAAAGAAGGCTCTGTTAAGTTTGAACACCACAAGCTCAGCGGACGCAAGTGGGTGCATATTGTGCCAAAGGAAGGTCACGTGCTTCACCCCGTCTATGCAAATGGTGCGACTGCGCCATCGGCAGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L27

Protein Analysis

147

Amino Acids

15.8

Weight (kDa)

10.58

Isoelectric Point (pI)

23.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L27 PF01016 44 - 123 6.4e-37 Ribosomal L27 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 251
AccI GTMKAC 1 cut(s) 66
AciI CCGC 2 cut(s) 207, 351
AclWI GGATC 3 cut(s) 144, 157, 300
AcsI RAATTY 3 cut(s) 4, 182, 200
AcuI CTGAAG 1 cut(s) 59
AcvI CACGTG 1 cut(s) 391
AfaI GTAC 1 cut(s) 85
AfiI CCNNNNNNNGG 1 cut(s) 383
AgsI TTSAA 2 cut(s) 174, 335
AjnI CCWGG 2 cut(s) 223, 265
AluBI AGCT 1 cut(s) 346
AluI AGCT 1 cut(s) 346
AlwI GGATC 3 cut(s) 144, 157, 300
ApeKI GCWGC 1 cut(s) 434
ApoI RAATTY 3 cut(s) 4, 182, 200
AspLEI GCGC 2 cut(s) 126, 427
AsuHPI GGTGA 1 cut(s) 389
BaeI ACNNNNGTAYC 2 cut(s) 75, 108
BamHI GGATCC 1 cut(s) 149
BanI GGYRCC 1 cut(s) 251
BbrPI CACGTG 1 cut(s) 391
BccI CCATC 1 cut(s) 436
BceAI ACGGC 1 cut(s) 111
BciT130I CCWGG 2 cut(s) 225, 267
BfoI RGCGCY 1 cut(s) 127
BglI GCCNNNNNGGC 1 cut(s) 249
BisI GCNGC 1 cut(s) 435
BlpI GCTNAGC 1 cut(s) 347
BlsI GCNGC 1 cut(s) 436
Bme1390I CCNGG 2 cut(s) 225, 267
BmiI GGNNCC 2 cut(s) 151, 253
BmrFI CCNGG 2 cut(s) 225, 267
Bpu1102I GCTNAGC 1 cut(s) 347
BsaAI YACGTR 1 cut(s) 391
BsaBI GATNNNNATC 1 cut(s) 174
BsaXI ACNNNNNCTCC 4 cut(s) 43, 73, 201, 231
Bsc4I CCNNNNNNNGG 1 cut(s) 383
Bse8I GATNNNNATC 1 cut(s) 174
BseBI CCWGG 2 cut(s) 225, 267
BseGI GGATG 1 cut(s) 262
BseJI GATNNNNATC 1 cut(s) 174
BseLI CCNNNNNNNGG 1 cut(s) 383
BseMII CTCAG 1 cut(s) 361
BseYI CCCAGC 1 cut(s) 126
BshNI GGYRCC 1 cut(s) 251
BslI CCNNNNNNNGG 1 cut(s) 383
Bsp143I GATC 2 cut(s) 149, 292
Bsp1720I GCTNAGC 1 cut(s) 347
BspACI CCGC 2 cut(s) 207, 351
BspCNI CTCAG 1 cut(s) 360
BspLI GGNNCC 2 cut(s) 151, 253
BspPI GGATC 3 cut(s) 144, 157, 300
BspT107I GGYRCC 1 cut(s) 251
BssMI GATC 2 cut(s) 149, 292
BssNAI GTATAC 1 cut(s) 67
Bst1107I GTATAC 1 cut(s) 67
Bst2UI CCWGG 2 cut(s) 225, 267
BstAPI GCANNNNNTGC 2 cut(s) 373, 416
BstBAI YACGTR 1 cut(s) 391
BstDEI CTNAG 1 cut(s) 347
BstF5I GGATG 1 cut(s) 262
BstH2I RGCGCY 1 cut(s) 127
BstHHI GCGC 2 cut(s) 126, 427
BstKTI GATC 2 cut(s) 152, 295
BstMBI GATC 2 cut(s) 149, 292
BstMWI GCNNNNNNNGC 4 cut(s) 249, 373, 416, 431
BstNI CCWGG 2 cut(s) 225, 267
BstSCI CCNGG 2 cut(s) 223, 265
BstX2I RGATCY 1 cut(s) 149
BstYI RGATCY 1 cut(s) 149
BstZ17I GTATAC 1 cut(s) 67
BtsCI GGATG 1 cut(s) 262
BtsI GCAGTG 1 cut(s) 79
BtsIMutI CAGTG 1 cut(s) 79
CfoI GCGC 2 cut(s) 126, 427
CseI GACGC 1 cut(s) 363
Csp6I GTAC 1 cut(s) 84
CviJI RGCY 3 cut(s) 131, 321, 346
CviKI_1 RGCY 3 cut(s) 131, 321, 346
CviQI GTAC 1 cut(s) 84
DdeI CTNAG 1 cut(s) 347
DpnI GATC 2 cut(s) 151, 294
DpnII GATC 2 cut(s) 149, 292
EciI GGCGGA 1 cut(s) 222
Eco57I CTGAAG 1 cut(s) 59
Eco72I CACGTG 1 cut(s) 391
EcoRII CCWGG 2 cut(s) 223, 265
FaiI YATR 5 cut(s) 22, 67, 276, 369, 408
FblI GTMKAC 1 cut(s) 66
Fnu4HI GCNGC 1 cut(s) 435
FokI GGATG 1 cut(s) 249
Fsp4HI GCNGC 1 cut(s) 435
GlaI GCGC 2 cut(s) 125, 426
GluI GCNGC 1 cut(s) 435
GsaI CCCAGC 1 cut(s) 130
HaeII RGCGCY 1 cut(s) 127
HgaI GACGC 1 cut(s) 363
HhaI GCGC 2 cut(s) 126, 427
Hin6I GCGC 2 cut(s) 124, 425
HinP1I GCGC 2 cut(s) 124, 425
HinfI GANTC 1 cut(s) 170
HphI GGTGA 1 cut(s) 389
Hpy166II GTNNAC 1 cut(s) 67
Hpy188I TCNGA 2 cut(s) 39, 312
Hpy188III TCNNGA 1 cut(s) 167
Hpy8I GTNNAC 1 cut(s) 67
HpyAV CCTTC 4 cut(s) 95, 283, 311, 377
HpyCH4IV ACGT 1 cut(s) 390
HpyCH4V TGCA 4 cut(s) 11, 24, 367, 410
HpyF10VI GCNNNNNNNGC 4 cut(s) 249, 373, 416, 431
HpyF3I CTNAG 1 cut(s) 347
HpySE526I ACGT 1 cut(s) 390
HspAI GCGC 2 cut(s) 124, 425
Kzo9I GATC 2 cut(s) 149, 292
LpnPI CCDG 7 cut(s) 66, 112, 132, 210, 237, 252, 279
MaeII ACGT 1 cut(s) 390
MaeIII GTNAC 3 cut(s) 49, 91, 386
MalI GATC 2 cut(s) 151, 294
MboI GATC 2 cut(s) 149, 292
MboII GAAGA 2 cut(s) 52, 208
MflI RGATCY 1 cut(s) 149
MluCI AATT 4 cut(s) 4, 182, 200, 271
MnlI CCTC 1 cut(s) 242
MseI TTAA 1 cut(s) 327
MslI CAYNNNNRTG 1 cut(s) 19
MspA1I CMGCKG 1 cut(s) 351
MspR9I CCNGG 2 cut(s) 225, 267
MvaI CCWGG 2 cut(s) 225, 267
MwoI GCNNNNNNNGC 4 cut(s) 249, 373, 416, 431
NdeII GATC 2 cut(s) 149, 292
NlaIV GGNNCC 2 cut(s) 151, 253
NmuCI GTSAC 2 cut(s) 91, 386
PfeI GAWTC 1 cut(s) 170
PfoI TCCNGGA 1 cut(s) 265
PkrI GCNGC 1 cut(s) 436
PmaCI CACGTG 1 cut(s) 391
PmlI CACGTG 1 cut(s) 391
Ppu21I YACGTR 1 cut(s) 391
Psp6I CCWGG 2 cut(s) 223, 265
PspCI CACGTG 1 cut(s) 391
PspFI CCCAGC 1 cut(s) 126
PspGI CCWGG 2 cut(s) 223, 265
PspN4I GGNNCC 2 cut(s) 151, 253
PsuI RGATCY 1 cut(s) 149
RsaI GTAC 1 cut(s) 85
RsaNI GTAC 1 cut(s) 84
RseI CAYNNNNRTG 1 cut(s) 19
SaqAI TTAA 1 cut(s) 327
SatI GCNGC 1 cut(s) 435
Sau3AI GATC 2 cut(s) 149, 292
ScrFI CCNGG 2 cut(s) 225, 267
SetI ASST 6 cut(s) 32, 119, 226, 348, 388, 393
SmiMI CAYNNNNRTG 1 cut(s) 19
Sse9I AATT 4 cut(s) 4, 182, 200, 271
SsiI CCGC 2 cut(s) 207, 351
StyD4I CCNGG 2 cut(s) 223, 265
TaiI ACGT 1 cut(s) 393
TaqI TCGA 1 cut(s) 166
TasI AATT 4 cut(s) 4, 182, 200, 271
TfiI GAWTC 1 cut(s) 170
Tru1I TTAA 1 cut(s) 327
Tru9I TTAA 1 cut(s) 327
TscAI CASTG 1 cut(s) 79
TseFI GTSAC 2 cut(s) 91, 386
TseI GCWGC 1 cut(s) 434
Tsp45I GTSAC 2 cut(s) 91, 386
TspDTI ATGAA 2 cut(s) 17, 251
TspRI CASTG 1 cut(s) 79
XapI RAATTY 3 cut(s) 4, 182, 200
XmiI GTMKAC 1 cut(s) 66
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.