pycom07g09560

Nsp1-like C-terminal region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0013006
Physical Location & Seq
Forward (+)
8840733 .. 8846736
6004 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2178 bp
ATGTCGGGCTTTTCATCCGGATCCTCCGCCTCCTCCGCCTCCTCCGCCTTTTCCTCTTCCGCCCAATCCACTTCCCCTTTCTCTTTCTCCAACCCTCCTTCCTCCTTCTCATTCGGCTCTTCTGCCTCCCTATTCGGATCGTCTGCACCTGCCACCACGTCCACCACCGGCTTCTCCATTGGATCCTCGGCCTTCGGCGCCTCCACTCCCTCTAACCCTTCCTCCTCCCCATCTCCATTCTCCTTCTCCCTCAACTCCACTTCCACTCCCTCCTCCTCCCCATTCGGATCCTCAAACCCTAGCTCCGCCTCTACCCCTTCCTTCGGTTTCGCACCCACCGCCACCACCACCACCACAATAACGTCTTCGGCTTCCGCCCCTTCTTTCGGATTCGTACCCTCCTCTACCAACACCACAACCTCATCTGCCTCCGCTTCCTTTGGATTCTCATCCGCCGCCGCCACCACCACAACCTCCTCTGCTTCCGCTCCTTCTTTTGGATTCTCATCAGCCGCATCCTCTGGCCTGTCCCTGTTTGGCTCCTCGGCCTCAACGGCTTCAACTCCTCTGTTTGGTTCAACTCCGTCCCAATCCCTGTTTGGCTCATCCTCATCGGCCCCCACCTCCACCGCCTCCGCCTCCCCTCTATTCGGAGCTCCTTCTTCCGCCACTTCATCCCCATTCGGAGCAACCTCATTTGCACCCAGCGCACCCACAACCCTGTTTGGAAGCTCGTCCACTTCCGCTTCGCAGCTGTTTGGCACAGCGTCCTCTGCACCCAGCGCACCCTCGCCACTGTTTGGCACAGCTTCCTCGTCCCAACCTTCATCCTCTGTAGCTCCAACCACCCAATCGTTCCAAAGCTTCTCCACTTCCACTTCAGCCTTCACATTACCAACATCCTCCCCTCTTTTGAAACCCACAACCTCCACTCCCACCACCGCTGCTCCTCCATCAACAACCGCTTCCAGCTTCTCCTCCTTCGGAACGCCGGCTTCCTCCGCTTCACAATCCTCTTTCGGTTTCTCCACCAGTTTCTCCAATGCCGCCTCAAAACCGACCTCACAATCCTTCCCAACTCCCTCCGCTCCGTTTCTTTCTACTCTCACGACCACAACCCCTTCTTCGACTCCGGCAACTAGCACTACAACCCAAGCCTCTTTCACTATGCCGTCTTTTGCTGTGATTCCTGCTACTTCTCCGGCTTCTAGCACCGCTGCAACAACGGCTCCAATGGCGGCCCCTGCGAGCTCAGCAGCTTCTTCCGGGGGCTCGCTTGTAAGCTCGTTCTCCTTCCAAACTACTTCAGGAGCTACTGCTTCTTCTGCTGGCTTTTTGTCTTTGACAAAAGGGTCTACACCTGCTCCATCGTCTCAAGCACAACCAAGCACTACCACGCCTGCATTGGGTGTCGGTACTTCAGCCCCTGTAACTGCTACAACAACCACTACAAGTACTGCTGCAACTCAGACATCAACATCACTTGTTGCAGCTTCTACTAGTGGGCTCGGATCTTTAGAAATTATGGAGTTCTATCTGACTTGTGCTTTTCTGCCCTGCTTGTTCTATAGGACAACTACAAATGTCAACACTACAGTATCTACTGCACCAAAATTACCGTCTGAAATCACAGGGAAGACTGTGGAGGAGGCTAATGCAATAGCAGATTGGGACAAAAGGATTTTGCACAACCGTGATGTTCTTCTTAGGCTTGAGATTGAAGTGGCGAAAGTGGTTGAAACCCAAGCTAACTTGGAGCGGCAACTAGAGTTAATTGAGACTCATCAGCAAGAGGTTGATAAGGCTTTGCAAAGCATGGAAGAAGAAGCTGAGCGTATATACAAGGATGAACATGGATTGCTCCGTGATGATGAGGCTGCATCCACAAGAGATGCAATGTATGAGCAAGCTGAGTTTGTAGAGAGGGAACTAGAGCAGGTGACAGAACAGATCAAATCAATTATTCAGACCTTAAATGCCAACCAGGGAGGGGAACTTGATGCAAATGATGGTATGGCTCCGTTAGATGTGGTTGTCCGAATCTTAAACAATCAGCTTAGCTCTTTAATGTGGATTGATGAAAAGGCTGAAGAATTCTCCTCGCGAATTCAGAAGCTCGCCAATGAAGGTCCTGCTGCAGATCGCGAATTAGTGGCTCCAAAATACTGGATGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0003674 GO:0005102 GO:0005198 GO:0005488 GO:0005515 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005642 GO:0005643 GO:0005737 GO:0005829 GO:0006139 GO:0006351 GO:0006355 GO:0006403 GO:0006405 GO:0006606 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0007049 GO:0007154 GO:0007165 GO:0007166 GO:0007276 GO:0007283 GO:0008092 GO:0008104 GO:0008134 GO:0008150 GO:0008152 GO:0008219 GO:0008285 GO:0008289 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009967 GO:0009968 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010648 GO:0010928 GO:0010930 GO:0010941 GO:0012505 GO:0015031 GO:0015833 GO:0015931 GO:0016020 GO:0016043 GO:0016070 GO:0017038 GO:0017056 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019894 GO:0019904 GO:0019953 GO:0022414 GO:0022607 GO:0023051 GO:0023052 GO:0023056 GO:0023057 GO:0030159 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031503 GO:0031965 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032182 GO:0032386 GO:0032501 GO:0032504 GO:0032774 GO:0032879 GO:0032880 GO:0032947 GO:0032991 GO:0033036 GO:0033157 GO:0033365 GO:0034399 GO:0034504 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0035257 GO:0042127 GO:0042169 GO:0042175 GO:0042306 GO:0042886 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043122 GO:0043123 GO:0043130 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044613 GO:0044703 GO:0045184 GO:0045893 GO:0045935 GO:0046483 GO:0046822 GO:0046907 GO:0046966 GO:0048232 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0048609 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051168 GO:0051169 GO:0051170 GO:0051171 GO:0051173 GO:0051179 GO:0051223 GO:0051234 GO:0051236 GO:0051252 GO:0051254 GO:0051259 GO:0051291 GO:0051427 GO:0051641 GO:0051649 GO:0051704 GO:0051716 GO:0060255 GO:0060341 GO:0060548 GO:0065003 GO:0065007 GO:0070013 GO:0070201 GO:0070206 GO:0070208 GO:0070727 GO:0071166 GO:0071426 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0080090 GO:0090087 GO:0090304 GO:0097659 GO:0098589 GO:0098805 GO:1900180 GO:1901360 GO:1901362 GO:1901576 GO:1902531 GO:1902533 GO:1902680 GO:1903506 GO:1903508 GO:1903827 GO:1904589 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
Nsp1_C Nucleoporin_FG

Protein Analysis

726

Amino Acids

73.06

Weight (kDa)

4.61

Isoelectric Point (pI)

58.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nsp1_C PF05064 549 - 617 2.8e-09 Nsp1-like C-terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013006)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45000
fragaria_vesca FvH4_7g10470 FvH4_7g10470 FvH4_7g10470
malus_domestica MD07G1110700.v1.1
prunus_persica Prupe.2G134200_v2.0.a1 Prupe.2G134200_v2.0.a1
pyrus_communis pycom07g09560
rosa_chinensis RchiOBHm_Chr1g0348261
rosa_laevigata RLG00000028672 RLG00000028676
rosa_roxburghii Rroxscaffold_4G00307070
rosa_rugosa Rorug01G0194000
rosa_samantha Rh1AG211000 Rh1BG177500 Rh1CG195400 Rh1DG207000
rosa_wichuraiana Rw1G017820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 157, 1369, 1927
AasI GACNNNNNNGTC 1 cut(s) 1351
Acc36I ACCTGC 3 cut(s) 157, 1369, 1927
AccB1I GGYRCC 1 cut(s) 197
AccB7I CCANNNNNTGG 1 cut(s) 800
AccBSI CCGCTC 3 cut(s) 488, 1088, 1759
AccI GTMKAC 1 cut(s) 1355
AccII CGCG 2 cut(s) 2104, 2145
AccIII TCCGGA 1 cut(s) 17
AclWI GGATC 8 cut(s) 15, 28, 145, 177, 190, 282, 295, 1519
AcsI RAATTY 2 cut(s) 2093, 2106
AcuI CTGAAG 4 cut(s) 864, 1290, 1404, 2109
AcyI GRCGYC 1 cut(s) 198
AfaI GTAC 3 cut(s) 396, 1417, 1456
AfiI CCNNNNNNNGG 8 cut(s) 323, 386, 497, 572, 650, 800, 1406, 1990
AgsI TTSAA 5 cut(s) 561, 579, 916, 1721, 1739
AhlI ACTAGT 1 cut(s) 1499
AjiI CACGTC 1 cut(s) 159
AjnI CCWGG 1 cut(s) 1983
AleI CACNNNNGTG 1 cut(s) 1692
Alw21I GWGCWC 2 cut(s) 658, 1253
Alw26I GTCTC 2 cut(s) 1377, 1772
AlwI GGATC 8 cut(s) 15, 28, 145, 177, 190, 282, 295, 1519
AlwNI CAGNNNCTG 1 cut(s) 1427
Aor13HI TCCGGA 1 cut(s) 17
AoxI GGCC 5 cut(s) 189, 523, 546, 615, 1239
ApeKI GCWGC 8 cut(s) 751, 944, 1217, 1256, 1460, 1490, 1877, 2135
ApoI RAATTY 2 cut(s) 2093, 2106
ArsI GACNNNNNNTTYG 2 cut(s) 1604, 1636
AspLEI GCGC 3 cut(s) 200, 710, 785
AspS9I GGNCC 3 cut(s) 616, 1240, 2129
AsuC2I CCSGG 1 cut(s) 1267
AsuHPI GGTGA 1 cut(s) 1951
AvaII GGWCC 1 cut(s) 2129
BaeI ACNNNNGTAYC 2 cut(s) 1581, 1614
BamHI GGATCC 3 cut(s) 20, 182, 287
BanI GGYRCC 1 cut(s) 197
BanII GRGCYC 4 cut(s) 658, 1253, 1274, 1509
BbsI GAAGAC 2 cut(s) 357, 1643
Bbv12I GWGCWC 2 cut(s) 658, 1253
BbvI GCAGC 8 cut(s) 763, 931, 1204, 1268, 1447, 1502, 1864, 2122
BccI CCATC 4 cut(s) 238, 961, 1375, 2003
BceAI ACGGC 3 cut(s) 570, 1156, 1242
BciT130I CCWGG 1 cut(s) 1985
BcnI CCSGG 1 cut(s) 1267
BcoDI GTCTC 2 cut(s) 1377, 1772
BcuI ACTAGT 1 cut(s) 1499
BfaI CTAG 6 cut(s) 300, 1140, 1209, 1500, 1766, 1931
BfmI CTRYAG 4 cut(s) 834, 1567, 1593, 2136
BfoI RGCGCY 1 cut(s) 201
BfuAI ACCTGC 3 cut(s) 157, 1369, 1927
BglI GCCNNNNNGGC 1 cut(s) 554
BlpI GCTNAGC 3 cut(s) 1252, 1830, 2057
BmcAI AGTACT 1 cut(s) 1456
Bme1390I CCNGG 2 cut(s) 1267, 1985
Bme18I GGWCC 1 cut(s) 2129
BmgBI CACGTC 1 cut(s) 159
BmgT120I GGNCC 3 cut(s) 616, 1240, 2129
BmrFI CCNGG 2 cut(s) 1267, 1985
BmsI GCATC 4 cut(s) 524, 1882, 1889, 1990
BpiI GAAGAC 2 cut(s) 357, 1643
Bpu1102I GCTNAGC 3 cut(s) 1252, 1830, 2057
BpuEI CTTGAG 2 cut(s) 1359, 1733
BpuMI CCSGG 1 cut(s) 1267
BsaBI GATNNNNATC 2 cut(s) 448, 505
BsaHI GRCGYC 1 cut(s) 198
BsaJI CCNNGG 4 cut(s) 186, 543, 1266, 1984
BsaWI WCCGGW 1 cut(s) 17
Bsc4I CCNNNNNNNGG 8 cut(s) 323, 386, 497, 572, 650, 800, 1406, 1990
Bse118I RCCGGY 2 cut(s) 167, 991
Bse1I ACTGG 2 cut(s) 1032, 2171
Bse3DI GCAATG 1 cut(s) 1902
Bse8I GATNNNNATC 2 cut(s) 448, 505
BseAI TCCGGA 1 cut(s) 17
BseBI CCWGG 1 cut(s) 1985
BseDI CCNNGG 4 cut(s) 186, 543, 1266, 1984
BseJI GATNNNNATC 2 cut(s) 448, 505
BseLI CCNNNNNNNGG 8 cut(s) 323, 386, 497, 572, 650, 800, 1406, 1990
BseMI GCAATG 1 cut(s) 1902
BseMII CTCAG 4 cut(s) 1266, 1481, 1821, 1902
BseNI ACTGG 2 cut(s) 1032, 2171
BseXI GCAGC 8 cut(s) 763, 931, 1204, 1268, 1447, 1502, 1864, 2122
BseYI CCCAGC 2 cut(s) 704, 779
BsgI GTGCAG 3 cut(s) 129, 759, 1590
Bsh1236I CGCG 2 cut(s) 2104, 2145
BshFI GGCC 5 cut(s) 191, 525, 548, 617, 1241
BshNI GGYRCC 1 cut(s) 197
BsiHKAI GWGCWC 2 cut(s) 658, 1253
BsiSI CCGG 6 cut(s) 18, 168, 992, 1133, 1202, 1266
BslFI GGGAC 4 cut(s) 514, 571, 802, 1685
BslI CCNNNNNNNGG 8 cut(s) 323, 386, 497, 572, 650, 800, 1406, 1990
BsmAI GTCTC 2 cut(s) 1377, 1772
BsmBI CGTCTC 1 cut(s) 1377
BsmFI GGGAC 4 cut(s) 514, 571, 802, 1685
BsnI GGCC 5 cut(s) 191, 525, 548, 617, 1241
Bsp1286I GDGCHC 4 cut(s) 658, 1253, 1274, 1509
Bsp13I TCCGGA 1 cut(s) 17
Bsp143I GATC 7 cut(s) 20, 137, 182, 287, 1511, 1950, 2140
Bsp1720I GCTNAGC 3 cut(s) 1252, 1830, 2057
Bsp68I TCGCGA 2 cut(s) 2104, 2145
BspANI GGCC 5 cut(s) 191, 525, 548, 617, 1241
BspCNI CTCAG 4 cut(s) 1265, 1480, 1822, 1903
BspEI TCCGGA 1 cut(s) 17
BspFNI CGCG 2 cut(s) 2104, 2145
BspMAI CTGCAG 1 cut(s) 2140
BspMI ACCTGC 3 cut(s) 157, 1369, 1927
BspPI GGATC 8 cut(s) 15, 28, 145, 177, 190, 282, 295, 1519
BspQI GCTCTTC 1 cut(s) 124
BspT107I GGYRCC 1 cut(s) 197
BsrBI CCGCTC 3 cut(s) 488, 1088, 1759
BsrDI GCAATG 1 cut(s) 1902
BsrFI RCCGGY 2 cut(s) 167, 991
BsrI ACTGG 2 cut(s) 1032, 2171
BssAI RCCGGY 2 cut(s) 167, 991
BssECI CCNNGG 4 cut(s) 186, 543, 1266, 1984
BssMI GATC 7 cut(s) 20, 137, 182, 287, 1511, 1950, 2140
BssNI GRCGYC 1 cut(s) 198
Bst2UI CCWGG 1 cut(s) 1985
Bst4CI ACNGT 5 cut(s) 798, 1597, 1620, 1642, 1694
Bst6I CTCTTC 2 cut(s) 61, 124
BstACI GRCGYC 1 cut(s) 198
BstC8I GCNNGC 7 cut(s) 993, 1249, 1274, 1330, 1401, 1908, 2118
BstDEI CTNAG 6 cut(s) 1252, 1467, 1706, 1830, 1911, 2057
BstFNI CGCG 2 cut(s) 2104, 2145
BstH2I RGCGCY 1 cut(s) 201
BstHHI GCGC 3 cut(s) 200, 710, 785
BstKTI GATC 7 cut(s) 23, 140, 185, 290, 1514, 1953, 2143
BstMAI GTCTC 2 cut(s) 1377, 1772
BstMBI GATC 7 cut(s) 20, 137, 182, 287, 1511, 1950, 2140
BstNI CCWGG 1 cut(s) 1985
BstSCI CCNGG 2 cut(s) 1265, 1983
BstSFI CTRYAG 4 cut(s) 834, 1567, 1593, 2136
BstUI CGCG 2 cut(s) 2104, 2145
BstV1I GCAGC 8 cut(s) 763, 931, 1204, 1268, 1447, 1502, 1864, 2122
BstV2I GAAGAC 2 cut(s) 357, 1643
BstX2I RGATCY 4 cut(s) 20, 182, 287, 1511
BstXI CCANNNNNNTGG 1 cut(s) 2166
BstYI RGATCY 4 cut(s) 20, 182, 287, 1511
BsuRI GGCC 5 cut(s) 191, 525, 548, 617, 1241
BtrI CACGTC 1 cut(s) 159
BtsIMutI CAGTG 1 cut(s) 794
BtuMI TCGCGA 2 cut(s) 2104, 2145
BveI ACCTGC 3 cut(s) 157, 1369, 1927
Cac8I GCNNGC 7 cut(s) 993, 1249, 1274, 1330, 1401, 1908, 2118
CaiI CAGNNNCTG 1 cut(s) 1427
CfoI GCGC 3 cut(s) 200, 710, 785
Cfr10I RCCGGY 2 cut(s) 167, 991
Cfr13I GGNCC 3 cut(s) 616, 1240, 2129
CseI GACGC 1 cut(s) 756
Csp6I GTAC 3 cut(s) 395, 1416, 1455
CviAII CATG 2 cut(s) 1816, 1853
CviQI GTAC 3 cut(s) 395, 1416, 1455
DdeI CTNAG 6 cut(s) 1252, 1467, 1706, 1830, 1911, 2057
DinI GGCGCC 1 cut(s) 199
DpnI GATC 7 cut(s) 22, 139, 184, 289, 1513, 1952, 2142
DpnII GATC 7 cut(s) 20, 137, 182, 287, 1511, 1950, 2140
DrdI GACNNNNNNGTC 1 cut(s) 1351
DseDI GACNNNNNNGTC 1 cut(s) 1351
Eam1104I CTCTTC 2 cut(s) 61, 124
EarI CTCTTC 2 cut(s) 61, 124
EciI GGCGGA 9 cut(s) 16, 25, 34, 49, 295, 364, 442, 625, 655
Ecl136II GAGCTC 2 cut(s) 656, 1251
Eco24I GRGCYC 4 cut(s) 658, 1253, 1274, 1509
Eco47I GGWCC 1 cut(s) 2129
Eco53kI GAGCTC 2 cut(s) 656, 1251
Eco57I CTGAAG 4 cut(s) 864, 1290, 1404, 2109
EcoICRI GAGCTC 2 cut(s) 656, 1251
EcoO109I RGGNCCY 1 cut(s) 2129
EcoRI GAATTC 2 cut(s) 2093, 2106
EcoRII CCWGG 1 cut(s) 1983
EcoT38I GRGCYC 4 cut(s) 658, 1253, 1274, 1509
EgeI GGCGCC 1 cut(s) 199
EheI GGCGCC 1 cut(s) 199
Esp3I CGTCTC 1 cut(s) 1377
FaeI CATG 2 cut(s) 1819, 1856
FaiI YATR 9 cut(s) 1169, 1526, 1569, 1817, 1838, 1840, 1854, 1902, 2015
FaqI GGGAC 4 cut(s) 514, 571, 802, 1685
FatI CATG 2 cut(s) 1815, 1852
FblI GTMKAC 1 cut(s) 1355
FokI GGATG 8 cut(s) 436, 502, 592, 661, 814, 886, 1859, 1867
FriOI GRGCYC 4 cut(s) 658, 1253, 1274, 1509
FspBI CTAG 6 cut(s) 300, 1140, 1209, 1500, 1766, 1931
GlaI GCGC 3 cut(s) 199, 709, 784
GsaI CCCAGC 2 cut(s) 708, 783
HaeII RGCGCY 1 cut(s) 201
HaeIII GGCC 5 cut(s) 191, 525, 548, 617, 1241
HapII CCGG 6 cut(s) 18, 168, 992, 1133, 1202, 1266
HgaI GACGC 1 cut(s) 756
HhaI GCGC 3 cut(s) 200, 710, 785
Hin1I GRCGYC 1 cut(s) 198
Hin1II CATG 2 cut(s) 1819, 1856
Hin6I GCGC 3 cut(s) 198, 708, 783
HinP1I GCGC 3 cut(s) 198, 708, 783
HincII GTYRAC 1 cut(s) 1588
HindII GTYRAC 1 cut(s) 1588
HindIII AAGCTT 1 cut(s) 862
HinfI GANTC 7 cut(s) 390, 444, 501, 1129, 1186, 1780, 2040
HpaII CCGG 6 cut(s) 18, 168, 992, 1133, 1202, 1266
HphI GGTGA 1 cut(s) 1951
Hpy166II GTNNAC 4 cut(s) 162, 738, 1356, 1588
Hpy188III TCNNGA 5 cut(s) 18, 1108, 1308, 2103, 2144
Hpy8I GTNNAC 4 cut(s) 162, 738, 1356, 1588
HpyCH4III ACNGT 5 cut(s) 798, 1597, 1620, 1642, 1694
HpyCH4IV ACGT 2 cut(s) 158, 362
HpyF3I CTNAG 6 cut(s) 1252, 1467, 1706, 1830, 1911, 2057
HpySE526I ACGT 2 cut(s) 158, 362
Hsp92I GRCGYC 1 cut(s) 198
Hsp92II CATG 2 cut(s) 1819, 1856
HspAI GCGC 3 cut(s) 198, 708, 783
KasI GGCGCC 1 cut(s) 197
Kpn2I TCCGGA 1 cut(s) 17
KroI GCCGGC 1 cut(s) 991
KroNI GCCGGC 1 cut(s) 993
Kzo9I GATC 7 cut(s) 20, 137, 182, 287, 1511, 1950, 2140
LguI GCTCTTC 1 cut(s) 124
Lsp1109I GCAGC 8 cut(s) 763, 931, 1204, 1268, 1447, 1502, 1864, 2122
LweI GCATC 4 cut(s) 524, 1882, 1889, 1990
MaeI CTAG 6 cut(s) 300, 1140, 1209, 1500, 1766, 1931
MaeII ACGT 2 cut(s) 158, 362
MaeIII GTNAC 2 cut(s) 1429, 1939
MalI GATC 7 cut(s) 22, 139, 184, 289, 1513, 1952, 2142
MbiI CCGCTC 3 cut(s) 488, 1088, 1759
MboI GATC 7 cut(s) 20, 137, 182, 287, 1511, 1950, 2140
MflI RGATCY 4 cut(s) 20, 182, 287, 1511
MhlI GDGCHC 4 cut(s) 658, 1253, 1274, 1509
MluCI AATT 7 cut(s) 1521, 1613, 1773, 1959, 2093, 2106, 2147
Mly113I GGCGCC 1 cut(s) 198
MlyI GAGTC 2 cut(s) 1123, 1774
MmeI TCCRAC 2 cut(s) 114, 866
MroI TCCGGA 1 cut(s) 17
MroNI GCCGGC 1 cut(s) 991
MseI TTAA 4 cut(s) 1772, 1973, 2045, 2066
MslI CAYNNNNRTG 1 cut(s) 1692
MspA1I CMGCKG 3 cut(s) 754, 944, 1217
MspI CCGG 6 cut(s) 18, 168, 992, 1133, 1202, 1266
MspR9I CCNGG 2 cut(s) 1267, 1985
MvaI CCWGG 1 cut(s) 1985
MvnI CGCG 2 cut(s) 2104, 2145
NaeI GCCGGC 1 cut(s) 993
NarI GGCGCC 1 cut(s) 198
NciI CCSGG 1 cut(s) 1267
NdeII GATC 7 cut(s) 20, 137, 182, 287, 1511, 1950, 2140
NgoMIV GCCGGC 1 cut(s) 991
NlaIII CATG 2 cut(s) 1819, 1856
NmeAIII GCCGAG 2 cut(s) 167, 524
NmuCI GTSAC 1 cut(s) 1939
NruI TCGCGA 2 cut(s) 2104, 2145
OliI CACNNNNGTG 1 cut(s) 1692
PaqCI CACCTGC 3 cut(s) 157, 1369, 1927
PciSI GCTCTTC 1 cut(s) 124
PdiI GCCGGC 1 cut(s) 993
PfeI GAWTC 5 cut(s) 390, 444, 501, 1186, 2040
PflMI CCANNNNNTGG 1 cut(s) 800
PleI GAGTC 2 cut(s) 1123, 1774
PluTI GGCGCC 1 cut(s) 201
PpsI GAGTC 2 cut(s) 1123, 1774
PpuMI RGGWCCY 1 cut(s) 2129
Psp124BI GAGCTC 2 cut(s) 658, 1253
Psp5II RGGWCCY 1 cut(s) 2129
Psp6I CCWGG 1 cut(s) 1983
PspFI CCCAGC 2 cut(s) 704, 779
PspGI CCWGG 1 cut(s) 1983
PspPI GGNCC 3 cut(s) 616, 1240, 2129
PspPPI RGGWCCY 1 cut(s) 2129
PstI CTGCAG 1 cut(s) 2140
PstNI CAGNNNCTG 1 cut(s) 1427
PsuI RGATCY 4 cut(s) 20, 182, 287, 1511
PvuII CAGCTG 1 cut(s) 754
RruI TCGCGA 2 cut(s) 2104, 2145
RsaI GTAC 3 cut(s) 396, 1417, 1456
RsaNI GTAC 3 cut(s) 395, 1416, 1455
RseI CAYNNNNRTG 1 cut(s) 1692
SacI GAGCTC 2 cut(s) 658, 1253
SapI GCTCTTC 1 cut(s) 124
SaqAI TTAA 4 cut(s) 1772, 1973, 2045, 2066
Sau3AI GATC 7 cut(s) 20, 137, 182, 287, 1511, 1950, 2140
Sau96I GGNCC 3 cut(s) 616, 1240, 2129
ScaI AGTACT 1 cut(s) 1456
SchI GAGTC 2 cut(s) 1123, 1774
ScrFI CCNGG 2 cut(s) 1267, 1985
SduI GDGCHC 4 cut(s) 658, 1253, 1274, 1509
SfaNI GCATC 4 cut(s) 524, 1882, 1889, 1990
SfcI CTRYAG 4 cut(s) 834, 1567, 1593, 2136
SfoI GGCGCC 1 cut(s) 199
SinI GGWCC 1 cut(s) 2129
SmiMI CAYNNNNRTG 1 cut(s) 1692
SmlI CTYRAG 2 cut(s) 1374, 1712
SmoI CTYRAG 2 cut(s) 1374, 1712
SpeI ACTAGT 1 cut(s) 1499
Sse9I AATT 7 cut(s) 1521, 1613, 1773, 1959, 2093, 2106, 2147
SspDI GGCGCC 1 cut(s) 197
SspMI CTAG 6 cut(s) 300, 1140, 1209, 1500, 1766, 1931
SstI GAGCTC 2 cut(s) 658, 1253
StyD4I CCNGG 2 cut(s) 1265, 1983
TaaI ACNGT 5 cut(s) 798, 1597, 1620, 1642, 1694
TaiI ACGT 2 cut(s) 161, 365
TaqI TCGA 1 cut(s) 1127
TasI AATT 7 cut(s) 1521, 1613, 1773, 1959, 2093, 2106, 2147
TatI WGTACW 1 cut(s) 1454
TauI GCSGC 6 cut(s) 458, 461, 515, 1049, 1241, 1762
TfiI GAWTC 5 cut(s) 390, 444, 501, 1186, 2040
Tru1I TTAA 4 cut(s) 1772, 1973, 2045, 2066
Tru9I TTAA 4 cut(s) 1772, 1973, 2045, 2066
TscAI CASTG 1 cut(s) 801
TseFI GTSAC 1 cut(s) 1939
TseI GCWGC 8 cut(s) 751, 944, 1217, 1256, 1460, 1490, 1877, 2135
Tsp45I GTSAC 1 cut(s) 1939
TspDTI ATGAA 5 cut(s) 663, 816, 1863, 2094, 2139
TspGWI ACGGA 4 cut(s) 573, 1080, 1853, 2010
TspRI CASTG 1 cut(s) 801
Van91I CCANNNNNTGG 1 cut(s) 800
VpaK11BI GGWCC 1 cut(s) 2129
XapI RAATTY 2 cut(s) 2093, 2106
XcmI CCANNNNNNNNNTGG 2 cut(s) 596, 1402
XmiI GTMKAC 1 cut(s) 1355
XspI CTAG 6 cut(s) 300, 1140, 1209, 1500, 1766, 1931
ZrmI AGTACT 1 cut(s) 1456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.