Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr7

Orthogroup ID

OG0009110

Physical Location & Seq

Genomic Coordinates Reverse (-)

19492058 .. 19498721

Gene Structure 6664 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2736 bp

ATGAATTTCACTTTGTGTTCGTCATCATCGGAGCTCAGATATACTTTTGTTGAGGGGTTGCCATGTACAAACAAGAATATGGTGGACTCTGAGCCTTTTGTGAAGGTTGATAAGTTCAATAAGAAGGATAGTACTCGAGCATCTAATCACAATGCTAGAAAGCTGTTGATGTCTAGCAATTTGATGCCTCAGCGCTTAAAAGGATCCAATATGGGAAGGAAACATCTCAAGGAACCTGGTCGGAAACTGGAATTTGTGAGGACATTGCTGATTGACAACTATGACAGCTACACATACAATATATACCAGGAGCTGTCTGTCATTAATGGGGTGCCTCCTGTGGTCGTGCGGAATGATGAATTGACATGGAAAGACATTCGCTATTACTTGTATGAAGAAAATGCTTTTGACAATGTTGTTATATCACCTGGACCTGGTTCGCCAGCATGTCCAGCAGACATAGGAATATGTCTTCAGGTGCTGCTTGAATGCTGGGATATCCCTATTTTGGGCGTTTGCCTTGGACATCAGGCTTTGGGTTATGTGCATGGTGCTAAAGTTGTCCACGCATCTGAACCAGTCCATGGACGGTTGAGTGAAATTGAGCACAACAGGTGCAAACTTTTCAATGATATCCCTTCCGGCCACAACTCAGGATTTAAGGTGGTTCGGTACCATTCACTTGTTGTAGATGCTGAATCCCTCCCAGATGAACTCATTCCTATAGCATGGACTTACTCCAGGGATGCACTTTCTTTTATTGAAACCCAGAAGTCTGATGTTCCCTCAGAATTTGCTGTGGGATCTTTTCCAACAAAACCAAAAAATGGAAGTTTTTCGCCTTTCAGTTGTTCTGGCAAAGTGCGGAGCGAAAAGGTTCTCATGGGCATTGTGCATTCTACCCGGCCTCATTATGGTTTGCAGTTCCATCCAGAGAGTATTGCGACCTTTCATGGGAGGCAGATATTCAAGAATTTTAGAGAGATCACAGAGGACTATTGGCTGAATTCGAGGGCATCATTCATCAAGAAGCGGAATTTTGATTATACAGCGTGCATGCAGATGCCCAATGCTAGTCGACTCCTCACAGAAGTTCCTAGACATAGGCAACTAGTAAGTAATTCAGATGGCCAGCTATATAACAAGATTTCTAGAAGGAAGAACAGTGAAAGTAACACAAGTTCTTCTGGTGCGGTCAATATGGTAAACCTTTTGCATCCAGGCATTGGTGTTAAATATCTAAACTTGAGATGGAAGAGATTTAAGCATTTGGCTGGTCAAGTTGGTGGAGCAAAAAATATATTCTGTGCACTGTTTGGACATCATAAAGCTGAAAATACCTTTTGGTTGGACAGTTCATCGATAGAAAAGAGAAGAGCACGGTTTTCATTTATGGGAGGAAAAGGTGGAACTCTTTGGAAGCAGTTGACCTATAAATTATCAGATAGAAGTGATACGACTTTGAAGGGCAGTGGTTTTCTATCAGTTGAGGACGCTCAAGGCTCTAACAGAAACACATTTTTGGAAGAAGGGTTTCTTGATTTTTTGAAAAAGGAGCTCATGTCATTTCGGTACGATGAAAAGGATTATGAAGGCCTTCCATTTGAATTTCATGGTGGATATATTGGTTACATGGGGTATAGCCTTAAAGTTGAATGTGGTGCATCATCTAACTGCCACAAATCCAGGACTCCAGATGCTTGCTTTTTCTTTGCTGATAATCTTGTAGTTATCGATCACTGCTCCGACGATGTTTATATACTGTCTATTAACAAAGAATGCACAAGCACGACACCATGGTTGGACAATACAGAACAAAAGCTTCTCAGGTTAAAAGCTTCTGCTACAAATGAGGGAGAGGAGCCAACCTCACAGGCTTTTCGATCTTCTCAGTATCAAGGAAGTTTTGTTGTTGATAAATCAAGAGAGCAGTATATAAAAGATGTCGACAGGTGCCTGGAATACATTAAAGATGGTGAAAGCTACGAGTTATGTCTCACAACTCAGATGAGAAAAAGAATTGGGGAAATGGACTCATTAGGACTTTACCTTCACCTTAGGGAAAAAAATCCGGCACCATATGCTGCCTGGCTTAATTTTTCAAAGGAAAAGCTTTGTGTCTGCTGTTCTTCCCCAGAGCGGTTTCTACGGTTGGATAGAAATGGTGTACTAGAAGCAAAGCCCATTAAGGGTACTATAGCTCGTGGTGCAACACTAGAGGAAGATGAACAACGCAAACTACAACTGCAATACAGTGAAAAGGATCAAGCTGAAAATCTGATGATTGTTGATCTTCTAAGAAATGACCTTGGCCGTGTGTGTGAGCCCGGTTCTGTTCATGTGCCCCATCTTATGGATGTAGAATCATATGCTACTGTCCATACTATGGTGAGCACAATTCGCGGGAAAAAGCGGTCAGATGTAAGTGCAGTTGACTGTGTGAGAGCAGCATTTCCAGGCGGTTCAATGACAGGTGCACCAAAATTAAGATCAATGGAACTTCTTGACTCGATCGAAACTAGTCCACGAGGAATCTACTCAGGGTCCATTGGTTTTTTCTCATATAACCAGACATTCGATCTCAACATAGTTATCAGAACTGTTGTTGTACACGAGGGTGAAGCTTCAATAGGCGCGGGAGGAGCCATTGTTGCGTTATCAAATCCTGAAGACGAGTATGACGAAATGATGCTGAAAACGCGCGCCCCGGCAAAAGCTGTAACGGAATTCTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006575 GO:0006725 GO:0006732 GO:0006760 GO:0006766 GO:0006767 GO:0006807 GO:0008150 GO:0008152 GO:0008153 GO:0008483 GO:0008652 GO:0009058 GO:0009072 GO:0009073 GO:0009108 GO:0009110 GO:0009396 GO:0009507 GO:0009536 GO:0009987 GO:0016053 GO:0016740 GO:0016769 GO:0017144 GO:0018130 GO:0019438 GO:0019752 GO:0032787 GO:0034641 GO:0042364 GO:0042398 GO:0042537 GO:0042558 GO:0042559 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0043648 GO:0043650 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046417 GO:0046482 GO:0046483 GO:0046653 GO:0046654 GO:0046655 GO:0046656 GO:0046820 GO:0051186 GO:0051188 GO:0071704 GO:0072330 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00790 map00790

Pfam Domains

Protein Families

Anth_synt_I_N Chorismate_bind GATase

Protein Analysis

912

Amino Acids

102.61

Weight (kDa)

7.74

Isoelectric Point (pI)

47.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GATase	PF00117	89 - 249	1.7e-30	Glutamine amidotransferase class-I
GATase	PF00117	283 - 326	1.1e-07	Glutamine amidotransferase class-I
Anth_synt_I_N	PF04715	443 - 587	1.8e-15	Anthranilate synthase component I, N terminal region
Chorismate_bind	PF00425	642 - 899	1.3e-85	chorismate binding enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0009110)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G28880
fragaria_vesca	FvH4_7g19690
malus_domestica	MD01G1109300.v1.1 MD07G1175300.v1.1
prunus_persica	Prupe.2G214500_v2.0.a1
pyrus_communis	pycom01g13660 pycom07g16970
rosa_chinensis	RchiOBHm_Chr1g0363521
rosa_laevigata	RLG00000027559
rosa_multiflora	Rmu_ssc0000433.1_g000001
rosa_roxburghii	Rroxscaffold_4G00293090
rosa_rugosa	Rorug01G0308100
rosa_samantha	Rh1AG316800 Rh1BG280200 Rh1CG296300 Rh1DG311400 Rh5DG228600
rosa_wichuraiana	Rw0G000800 Rw1G028080 Rw2G022180 Rw3G029370 Rw5G038670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	672
AccB1I	GGYRCC	4 cut(s)	331, 672, 1953, 2074
AccB7I	CCANNNNNTGG	5 cut(s)	584, 827, 1226, 2353, 2386
AccBSI	CCGCTC	1 cut(s)	2140
AccI	GTMKAC	2 cut(s)	1078, 1947
AccII	CGCG	4 cut(s)	2403, 2636, 2701, 2703
AciI	CCGC	9 cut(s)	349, 865, 1033, 1193, 2140, 2403, 2413, 2460, 2636
AclWI	GGATC	4 cut(s)	198, 211, 811, 2271
AcoI	YGGCCR	3 cut(s)	643, 1129, 2311
AcsI	RAATTY	8 cut(s)	4, 251, 791, 973, 1006, 1036, 1607, 2726
AcuI	CTGAAG	2 cut(s)	458, 2688
AdeI	CACNNNGTG	1 cut(s)	15
AfaI	GTAC	7 cut(s)	67, 133, 674, 1574, 2169, 2194, 2610
AfeI	AGCGCT	1 cut(s)	194
AhlI	ACTAGT	2 cut(s)	1111, 2519
AleI	CACNNNNGTG	1 cut(s)	2616
Alw21I	GWGCWC	7 cut(s)	36, 609, 1312, 1381, 1560, 2396, 2479
Alw26I	GTCTC	1 cut(s)	2000
Alw44I	GTGCAC	2 cut(s)	1308, 2475
AlwI	GGATC	4 cut(s)	198, 211, 811, 2271
AlwNI	CAGNNNCTG	2 cut(s)	313, 481
Ama87I	CYCGRG	1 cut(s)	135
Aor51HI	AGCGCT	1 cut(s)	194
AoxI	GGCC	5 cut(s)	643, 905, 1129, 1594, 2311
ApaLI	GTGCAC	2 cut(s)	1308, 2475
ApeKI	GCWGC	3 cut(s)	481, 2084, 2447
ApoI	RAATTY	8 cut(s)	4, 251, 791, 973, 1006, 1036, 1607, 2726
AseI	ATTAAT	1 cut(s)	324
Asp700I	GAANNNNTTC	5 cut(s)	717, 835, 876, 1533, 1596
Asp718I	GGTACC	1 cut(s)	672
AspLEI	GCGC	4 cut(s)	195, 2636, 2703, 2705
AspS9I	GGNCC	2 cut(s)	431, 2544
AsuC2I	CCSGG	3 cut(s)	904, 2328, 2708
AsuHPI	GGTGA	5 cut(s)	417, 1988, 2045, 2401, 2630
AvaI	CYCGRG	1 cut(s)	135
AvaII	GGWCC	2 cut(s)	431, 2544
AxyI	CCTNAGG	1 cut(s)	2057
BaeGI	GKGCMC	3 cut(s)	1312, 2346, 2479
BalI	TGGCCA	1 cut(s)	1131
BamHI	GGATCC	1 cut(s)	203
BanI	GGYRCC	4 cut(s)	331, 672, 1953, 2074
BanII	GRGCYC	3 cut(s)	36, 1560, 2328
BauI	CACGAG	3 cut(s)	2202, 2526, 2612
BbsI	GAAGAC	2 cut(s)	464, 2676
Bbv12I	GWGCWC	7 cut(s)	36, 609, 1312, 1381, 1560, 2396, 2479
BbvCI	CCTCAGC	1 cut(s)	189
BbvI	GCAGC	3 cut(s)	468, 2071, 2459
BccI	CCATC	5 cut(s)	936, 1121, 1245, 1967, 2355
BceAI	ACGGC	1 cut(s)	2298
BcnI	CCSGG	3 cut(s)	904, 2328, 2708
BcoDI	GTCTC	1 cut(s)	2000
BcuI	ACTAGT	2 cut(s)	1111, 2519
BfaI	CTAG	9 cut(s)	156, 174, 1074, 1098, 1112, 1152, 2171, 2216, 2520
BfmI	CTRYAG	2 cut(s)	723, 2196
BfoI	RGCGCY	1 cut(s)	196
BisI	GCNGC	3 cut(s)	482, 2085, 2448
BlsI	GCNGC	3 cut(s)	483, 2086, 2449
BmcAI	AGTACT	1 cut(s)	133
Bme18I	GGWCC	2 cut(s)	431, 2544
BmeT110I	CYCGRG	1 cut(s)	135
BmgT120I	GGNCC	2 cut(s)	431, 2544
BmiI	GGNNCC	9 cut(s)	205, 234, 333, 674, 1863, 1955, 2076, 2545, 2644
BpiI	GAAGAC	2 cut(s)	464, 2676
BplI	GAGNNNNNCTC	2 cut(s)	1853, 1885
BpmI	CTGGAG	2 cut(s)	724, 1677
Bpu10I	CCTNAGC	1 cut(s)	189
BpuEI	CTTGAG	3 cut(s)	212, 1267, 1482
BpuMI	CCSGG	3 cut(s)	904, 2328, 2708
Bsa29I	ATCGAT	2 cut(s)	1361, 1734
BsaJI	CCNNGG	6 cut(s)	520, 583, 741, 1796, 2308, 2706
BsaXI	ACNNNNNCTCC	4 cut(s)	1547, 1577, 2634, 2664
Bse1I	ACTGG	2 cut(s)	252, 578
Bse21I	CCTNAGG	1 cut(s)	2057
Bse3DI	GCAATG	1 cut(s)	263
BseCI	ATCGAT	2 cut(s)	1361, 1734
BseDI	CCNNGG	6 cut(s)	520, 583, 741, 1796, 2308, 2706
BseGI	GGATG	4 cut(s)	751, 928, 1216, 2362
BseMI	GCAATG	1 cut(s)	263
BseMII	CTCAG	9 cut(s)	49, 81, 203, 666, 801, 1840, 1904, 2018, 2553
BseNI	ACTGG	2 cut(s)	252, 578
BsePI	GCGCGC	1 cut(s)	2701
BseRI	GAGGAG	3 cut(s)	1073, 1874, 2655
BseSI	GKGCMC	3 cut(s)	1312, 2346, 2479
BseXI	GCAGC	3 cut(s)	468, 2071, 2459
BseYI	CCCAGC	1 cut(s)	492
BsgI	GTGCAG	1 cut(s)	2448
Bsh1236I	CGCG	4 cut(s)	2403, 2636, 2701, 2703
Bsh1285I	CGRYCG	1 cut(s)	2514
BshFI	GGCC	5 cut(s)	645, 907, 1131, 1596, 2313
BshNI	GGYRCC	4 cut(s)	331, 672, 1953, 2074
BshVI	ATCGAT	2 cut(s)	1361, 1734
BsiEI	CGRYCG	1 cut(s)	2514
BsiHKAI	GWGCWC	7 cut(s)	36, 609, 1312, 1381, 1560, 2396, 2479
BsiHKCI	CYCGRG	1 cut(s)	135
BsiSI	CCGG	5 cut(s)	642, 904, 2072, 2328, 2708
BsmAI	GTCTC	1 cut(s)	2000
BsmI	GAATGC	3 cut(s)	494, 895, 1784
BsnI	GGCC	5 cut(s)	645, 907, 1131, 1596, 2313
BsoBI	CYCGRG	1 cut(s)	135
Bsp1286I	GDGCHC	9 cut(s)	36, 609, 1312, 1381, 1560, 2328, 2346, 2396, 2479
Bsp1407I	TGTACA	2 cut(s)	65, 2608
Bsp19I	CCATGG	2 cut(s)	583, 1796
BspACI	CCGC	9 cut(s)	349, 865, 1033, 1193, 2140, 2403, 2413, 2460, 2636
BspANI	GGCC	5 cut(s)	645, 907, 1131, 1596, 2313
BspCNI	CTCAG	9 cut(s)	48, 82, 202, 665, 800, 1839, 1903, 2017, 2552
BspDI	ATCGAT	2 cut(s)	1361, 1734
BspFNI	CGCG	4 cut(s)	2403, 2636, 2701, 2703
BspLI	GGNNCC	9 cut(s)	205, 234, 333, 674, 1863, 1955, 2076, 2545, 2644
BspPI	GGATC	4 cut(s)	198, 211, 811, 2271
BspQI	GCTCTTC	1 cut(s)	1369
BspT107I	GGYRCC	4 cut(s)	331, 672, 1953, 2074
BsrBI	CCGCTC	1 cut(s)	2140
BsrDI	GCAATG	1 cut(s)	263
BsrGI	TGTACA	2 cut(s)	65, 2608
BsrI	ACTGG	2 cut(s)	252, 578
BssECI	CCNNGG	6 cut(s)	520, 583, 741, 1796, 2308, 2706
BssHII	GCGCGC	1 cut(s)	2701
BssSI	CACGAG	3 cut(s)	2202, 2526, 2612
BssT1I	CCWWGG	4 cut(s)	520, 583, 1796, 2308
Bst2BI	CACGAG	3 cut(s)	2202, 2526, 2612
Bst6I	CTCTTC	2 cut(s)	1250, 1369
BstAPI	GCANNNNNTGC	1 cut(s)	2081
BstAUI	TGTACA	2 cut(s)	65, 2608
BstC8I	GCNNGC	6 cut(s)	444, 1054, 1058, 1133, 1702, 2703
BstDSI	CCRYGG	2 cut(s)	583, 1796
BstF5I	GGATG	4 cut(s)	751, 928, 1216, 2362
BstFNI	CGCG	4 cut(s)	2403, 2636, 2701, 2703
BstH2I	RGCGCY	1 cut(s)	196
BstHHI	GCGC	4 cut(s)	195, 2636, 2703, 2705
BstMAI	GTCTC	1 cut(s)	2000
BstMCI	CGRYCG	1 cut(s)	2514
BstMWI	GCNNNNNNNGC	7 cut(s)	452, 2081, 2207, 2400, 2642, 2651, 2698
BstNSI	RCATGY	2 cut(s)	450, 1060
BstSFI	CTRYAG	2 cut(s)	723, 2196
BstSLI	GKGCMC	3 cut(s)	1312, 2346, 2479
BstUI	CGCG	4 cut(s)	2403, 2636, 2701, 2703
BstV1I	GCAGC	3 cut(s)	468, 2071, 2459
BstV2I	GAAGAC	2 cut(s)	464, 2676
BstX2I	RGATCY	2 cut(s)	203, 803
BstYI	RGATCY	2 cut(s)	203, 803
Bsu15I	ATCGAT	2 cut(s)	1361, 1734
Bsu36I	CCTNAGG	1 cut(s)	2057
BsuRI	GGCC	5 cut(s)	645, 907, 1131, 1596, 2313
BsuTUI	ATCGAT	2 cut(s)	1361, 1734
BtgI	CCRYGG	2 cut(s)	583, 1796
BtsCI	GGATG	4 cut(s)	751, 928, 1216, 2362
BtsI	GCAGTG	2 cut(s)	1477, 1738
BtsIMutI	CAGTG	5 cut(s)	1171, 1310, 1477, 1738, 2260
Cac8I	GCNNGC	6 cut(s)	444, 1054, 1058, 1133, 1702, 2703
CaiI	CAGNNNCTG	2 cut(s)	313, 481
CfoI	GCGC	4 cut(s)	195, 2636, 2703, 2705
Cfr13I	GGNCC	2 cut(s)	431, 2544
ClaI	ATCGAT	2 cut(s)	1361, 1734
CseI	GACGC	1 cut(s)	1502
CsiI	ACCWGGT	2 cut(s)	235, 433
Csp6I	GTAC	7 cut(s)	66, 132, 673, 1573, 2168, 2193, 2609
CviQI	GTAC	7 cut(s)	66, 132, 673, 1573, 2168, 2193, 2609
DraIII	CACNNNGTG	1 cut(s)	15
EaeI	YGGCCR	3 cut(s)	643, 1129, 2311
Eam1104I	CTCTTC	2 cut(s)	1250, 1369
EarI	CTCTTC	2 cut(s)	1250, 1369
Ecl136II	GAGCTC	2 cut(s)	34, 1558
Eco130I	CCWWGG	4 cut(s)	520, 583, 1796, 2308
Eco147I	AGGCCT	1 cut(s)	1596
Eco24I	GRGCYC	3 cut(s)	36, 1560, 2328
Eco32I	GATATC	2 cut(s)	499, 634
Eco47I	GGWCC	2 cut(s)	431, 2544
Eco47III	AGCGCT	1 cut(s)	194
Eco53kI	GAGCTC	2 cut(s)	34, 1558
Eco57I	CTGAAG	2 cut(s)	458, 2688
Eco81I	CCTNAGG	1 cut(s)	2057
Eco88I	CYCGRG	1 cut(s)	135
EcoICRI	GAGCTC	2 cut(s)	34, 1558
EcoRI	GAATTC	2 cut(s)	1006, 2726
EcoRV	GATATC	2 cut(s)	499, 634
EcoT14I	CCWWGG	4 cut(s)	520, 583, 1796, 2308
EcoT38I	GRGCYC	3 cut(s)	36, 1560, 2328
ErhI	CCWWGG	4 cut(s)	520, 583, 1796, 2308
FalI	AAGNNNNNCTT	2 cut(s)	1521, 1553
FauI	CCCGC	2 cut(s)	2396, 2629
FauNDI	CATATG	2 cut(s)	2080, 2368
FblI	GTMKAC	2 cut(s)	1078, 1947
Fnu4HI	GCNGC	3 cut(s)	482, 2085, 2448
FokI	GGATG	4 cut(s)	758, 915, 1203, 2369
FriOI	GRGCYC	3 cut(s)	36, 1560, 2328
Fsp4HI	GCNGC	3 cut(s)	482, 2085, 2448
FspBI	CTAG	9 cut(s)	156, 174, 1074, 1098, 1112, 1152, 2171, 2216, 2520
GlaI	GCGC	4 cut(s)	194, 2635, 2702, 2704
GluI	GCNGC	3 cut(s)	482, 2085, 2448
GsaI	CCCAGC	1 cut(s)	496
GsuI	CTGGAG	2 cut(s)	724, 1677
HaeII	RGCGCY	1 cut(s)	196
HaeIII	GGCC	5 cut(s)	645, 907, 1131, 1596, 2313
HapII	CCGG	5 cut(s)	642, 904, 2072, 2328, 2708
HgaI	GACGC	1 cut(s)	1502
HhaI	GCGC	4 cut(s)	195, 2636, 2703, 2705
Hin6I	GCGC	4 cut(s)	193, 2634, 2701, 2703
HinP1I	GCGC	4 cut(s)	193, 2634, 2701, 2703
HincII	GTYRAC	4 cut(s)	1079, 1428, 1948, 2434
HindII	GTYRAC	4 cut(s)	1079, 1428, 1948, 2434
HindIII	AAGCTT	4 cut(s)	1820, 1836, 2111, 2622
HinfI	GANTC	8 cut(s)	86, 698, 1080, 1690, 2033, 2363, 2507, 2532
HpaII	CCGG	5 cut(s)	642, 904, 2072, 2328, 2708
HphI	GGTGA	5 cut(s)	417, 1988, 2045, 2401, 2630
Hpy99I	CGWCG	1 cut(s)	1751
HpyF10VI	GCNNNNNNNGC	7 cut(s)	452, 2081, 2207, 2400, 2642, 2651, 2698
HspAI	GCGC	4 cut(s)	193, 2634, 2701, 2703
KpnI	GGTACC	1 cut(s)	676
LguI	GCTCTTC	1 cut(s)	1369
LmnI	GCTCC	8 cut(s)	31, 310, 867, 1289, 1555, 1748, 1861, 2642
Lsp1109I	GCAGC	3 cut(s)	468, 2071, 2459
MabI	ACCWGGT	2 cut(s)	235, 433
MaeI	CTAG	9 cut(s)	156, 174, 1074, 1098, 1112, 1152, 2171, 2216, 2520
MaeIII	GTNAC	3 cut(s)	1172, 1628, 2719
MbiI	CCGCTC	1 cut(s)	2140
MflI	RGATCY	2 cut(s)	203, 803
MhlI	GDGCHC	9 cut(s)	36, 609, 1312, 1381, 1560, 2328, 2346, 2396, 2479
MlsI	TGGCCA	1 cut(s)	1131
MluNI	TGGCCA	1 cut(s)	1131
MlyI	GAGTC	5 cut(s)	80, 1074, 1684, 2027, 2501
MmeI	TCCRAC	6 cut(s)	221, 836, 1329, 1770, 1782, 2133
Mox20I	TGGCCA	1 cut(s)	1131
MroXI	GAANNNNTTC	5 cut(s)	717, 835, 876, 1533, 1596
MscI	TGGCCA	1 cut(s)	1131
MslI	CAYNNNNRTG	2 cut(s)	1061, 2616
Msp20I	TGGCCA	1 cut(s)	1131
MspI	CCGG	5 cut(s)	642, 904, 2072, 2328, 2708
Mva1269I	GAATGC	3 cut(s)	494, 895, 1784
MvnI	CGCG	4 cut(s)	2403, 2636, 2701, 2703
MwoI	GCNNNNNNNGC	7 cut(s)	452, 2081, 2207, 2400, 2642, 2651, 2698
NciI	CCSGG	3 cut(s)	904, 2328, 2708
NcoI	CCATGG	2 cut(s)	583, 1796
NdeI	CATATG	2 cut(s)	2080, 2368
NlaIV	GGNNCC	9 cut(s)	205, 234, 333, 674, 1863, 1955, 2076, 2545, 2644
NspI	RCATGY	2 cut(s)	450, 1060
OliI	CACNNNNGTG	1 cut(s)	2616
PaeI	GCATGC	1 cut(s)	1060
PaeR7I	CTCGAG	1 cut(s)	135
PauI	GCGCGC	1 cut(s)	2701
PceI	AGGCCT	1 cut(s)	1596
PciSI	GCTCTTC	1 cut(s)	1369
PcsI	WCGNNNNNNNCGW	1 cut(s)	2679
PctI	GAATGC	3 cut(s)	494, 895, 1784
PdmI	GAANNNNTTC	5 cut(s)	717, 835, 876, 1533, 1596
PfeI	GAWTC	3 cut(s)	698, 2363, 2532
PflMI	CCANNNNNTGG	5 cut(s)	584, 827, 1226, 2353, 2386
PfoI	TCCNGGA	1 cut(s)	1685
PkrI	GCNGC	3 cut(s)	483, 2086, 2449
Ple19I	CGATCG	1 cut(s)	2514
PleI	GAGTC	5 cut(s)	80, 1074, 1684, 2027, 2501
PpsI	GAGTC	5 cut(s)	80, 1074, 1684, 2027, 2501
PshBI	ATTAAT	1 cut(s)	324
Psp124BI	GAGCTC	2 cut(s)	36, 1560
PspFI	CCCAGC	1 cut(s)	492
PspN4I	GGNNCC	9 cut(s)	205, 234, 333, 674, 1863, 1955, 2076, 2545, 2644
PspPI	GGNCC	2 cut(s)	431, 2544
PspXI	VCTCGAGB	1 cut(s)	135
PsrI	GAACNNNNNNTAC	2 cut(s)	1165, 1197
PstNI	CAGNNNCTG	2 cut(s)	313, 481
PsuI	RGATCY	2 cut(s)	203, 803
PteI	GCGCGC	1 cut(s)	2701
PvuI	CGATCG	1 cut(s)	2514
RsaI	GTAC	7 cut(s)	67, 133, 674, 1574, 2169, 2194, 2610
RsaNI	GTAC	7 cut(s)	66, 132, 673, 1573, 2168, 2193, 2609
RseI	CAYNNNNRTG	2 cut(s)	1061, 2616
SacI	GAGCTC	2 cut(s)	36, 1560
SalI	GTCGAC	2 cut(s)	1077, 1946
SapI	GCTCTTC	1 cut(s)	1369
SatI	GCNGC	3 cut(s)	482, 2085, 2448
Sau96I	GGNCC	2 cut(s)	431, 2544
ScaI	AGTACT	1 cut(s)	133
SchI	GAGTC	5 cut(s)	80, 1074, 1684, 2027, 2501
SduI	GDGCHC	9 cut(s)	36, 609, 1312, 1381, 1560, 2328, 2346, 2396, 2479
SexAI	ACCWGGT	2 cut(s)	235, 433
SfcI	CTRYAG	2 cut(s)	723, 2196
Sfr274I	CTCGAG	1 cut(s)	135
SinI	GGWCC	2 cut(s)	431, 2544
SlaI	CTCGAG	1 cut(s)	135
SmiMI	CAYNNNNRTG	2 cut(s)	1061, 2616
SmlI	CTYRAG	4 cut(s)	135, 227, 1246, 1497
SmoI	CTYRAG	4 cut(s)	135, 227, 1246, 1497
SpeI	ACTAGT	2 cut(s)	1111, 2519
SphI	GCATGC	1 cut(s)	1060
SseBI	AGGCCT	1 cut(s)	1596
SsiI	CCGC	9 cut(s)	349, 865, 1033, 1193, 2140, 2403, 2413, 2460, 2636
SspMI	CTAG	9 cut(s)	156, 174, 1074, 1098, 1112, 1152, 2171, 2216, 2520
SstI	GAGCTC	2 cut(s)	36, 1560
StuI	AGGCCT	1 cut(s)	1596
StyI	CCWWGG	4 cut(s)	520, 583, 1796, 2308
TatI	WGTACW	4 cut(s)	65, 131, 2167, 2608
TfiI	GAWTC	3 cut(s)	698, 2363, 2532
TscAI	CASTG	5 cut(s)	1171, 1317, 1477, 1745, 2260
TseI	GCWGC	3 cut(s)	481, 2084, 2447
TspRI	CASTG	5 cut(s)	1171, 1317, 1477, 1745, 2260
Van91I	CCANNNNNTGG	5 cut(s)	584, 827, 1226, 2353, 2386
VneI	GTGCAC	2 cut(s)	1308, 2475
VpaK11BI	GGWCC	2 cut(s)	431, 2544
VspI	ATTAAT	1 cut(s)	324
XapI	RAATTY	8 cut(s)	4, 251, 791, 973, 1006, 1036, 1607, 2726
XbaI	TCTAGA	1 cut(s)	1151
XceI	RCATGY	2 cut(s)	450, 1060
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2085
XhoI	CTCGAG	1 cut(s)	135
XmiI	GTMKAC	2 cut(s)	1078, 1947
XmnI	GAANNNNTTC	5 cut(s)	717, 835, 876, 1533, 1596
XspI	CTAG	9 cut(s)	156, 174, 1074, 1098, 1112, 1152, 2171, 2216, 2520
ZrmI	AGTACT	1 cut(s)	133

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.