pycom07g17340

RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0006332
Physical Location & Seq
Reverse (-)
19854788 .. 19856140
1353 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 696 bp
ATGACAAGCAGCAACAACGTTACTCATGGGCAGTTTGGTGATACCACACTGACCAAAGTCTTCGTCGGAGGGCTGGCGTGGGAGACTCCCAAAGAGGCCTTGAGAGAGCACTTCGACAAGTACGGCGAGATCTTGGAGGCCGTCATCATCTCCGACAAACTCACCGGCCGATCCAAGGGTTACGGTTTCGTCACCTTCAAGGAGCCGGAGGAGGCTAAGAAGGCTTGTGAGGACGCCACGCCGGTCATCAACGGCCGCCGTGCCAACTGCAACCTCGCTTCCCTCGGGGCTCGCCGCCCTAGGTCCGCTTCAAACACCACCCCTCCTCCTCAACGAGGATCGAACGGTGGCGGAGCGAGGTCCATGTCACCTGCTCCCGCACCTGCACCTGCAAATCACGTGCAGTGCTACTCCCCCACATACATTGCCGCTGATATGAGTTACAATCATAAGGTGGGCTACACAGGTGGAGCTTACATGAACGGGCATTACTCTGCTCCTCAAGTTTACCCAGCACAGCCAGTGGTGGGACACAACACTTTGATGCCAATGTATCCTCTCTACTACCACCATCACCATCAATCACATACAATGGGCTTACCTGCTCACATCTACTCGCCGACCATGGCCGGACACGTGGCCACAGTCCCGACCATTATGTCCAAACCAGCATCGATTGCTCCTAACACAGGTTGA

Protein Analysis

232

Amino Acids

24.79

Weight (kDa)

9.07

Isoelectric Point (pI)

59.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 20 - 77 5.9e-18 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 379, 391, 397
AasI GACNNNNNNGTC 1 cut(s) 658
Acc36I ACCTGC 4 cut(s) 379, 391, 397, 610
AciI CCGC 6 cut(s) 256, 295, 306, 351, 378, 429
AclI AACGTT 1 cut(s) 18
AclWI GGATC 2 cut(s) 165, 346
AcoI YGGCCR 4 cut(s) 166, 253, 627, 639
AcvI CACGTG 2 cut(s) 400, 637
AcyI GRCGYC 1 cut(s) 234
AfaI GTAC 1 cut(s) 122
AfiI CCNNNNNNNGG 4 cut(s) 175, 335, 527, 689
AflIII ACRYGT 1 cut(s) 634
AgsI TTSAA 2 cut(s) 199, 312
AluBI AGCT 1 cut(s) 473
AluI AGCT 1 cut(s) 473
Alw21I GWGCWC 1 cut(s) 111
Alw26I GTCTC 1 cut(s) 77
AlwI GGATC 2 cut(s) 165, 346
Ama87I CYCGRG 1 cut(s) 284
AoxI GGCC 6 cut(s) 96, 138, 166, 253, 627, 639
ApeKI GCWGC 1 cut(s) 9
ArsI GACNNNNNNTTYG 2 cut(s) 48, 80
AspA2I CCTAGG 1 cut(s) 299
AspS9I GGNCC 2 cut(s) 303, 360
AsuHPI GGTGA 5 cut(s) 50, 154, 184, 360, 566
AvaI CYCGRG 1 cut(s) 284
AvaII GGWCC 2 cut(s) 303, 360
AvrII CCTAGG 1 cut(s) 299
BalI TGGCCA 1 cut(s) 641
BanII GRGCYC 1 cut(s) 292
BbrPI CACGTG 2 cut(s) 400, 637
BbsI GAAGAC 1 cut(s) 52
Bbv12I GWGCWC 1 cut(s) 111
BbvI GCAGC 1 cut(s) 21
BccI CCATC 2 cut(s) 579, 585
BceAI ACGGC 4 cut(s) 125, 139, 243, 268
BciVI GTATCC 1 cut(s) 564
BcoDI GTCTC 1 cut(s) 77
BfaI CTAG 1 cut(s) 300
BfuAI ACCTGC 4 cut(s) 379, 391, 397, 610
BfuI GTATCC 1 cut(s) 564
BglII AGATCT 1 cut(s) 129
BisI GCNGC 4 cut(s) 10, 256, 295, 429
BlnI CCTAGG 1 cut(s) 299
BlsI GCNGC 4 cut(s) 11, 257, 296, 430
Bme18I GGWCC 2 cut(s) 303, 360
BmeT110I CYCGRG 1 cut(s) 284
BmgT120I GGNCC 2 cut(s) 303, 360
BmiI GGNNCC 1 cut(s) 204
BmsI GCATC 2 cut(s) 534, 680
BoxI GACNNNNGTC 1 cut(s) 56
BpiI GAAGAC 1 cut(s) 52
BpuEI CTTGAG 2 cut(s) 121, 486
Bsa29I ATCGAT 1 cut(s) 674
BsaAI YACGTR 2 cut(s) 400, 637
BsaHI GRCGYC 1 cut(s) 234
BsaJI CCNNGG 4 cut(s) 174, 283, 299, 624
BsaXI ACNNNNNCTCC 4 cut(s) 307, 310, 337, 340
Bsc4I CCNNNNNNNGG 4 cut(s) 175, 335, 527, 689
Bse118I RCCGGY 2 cut(s) 164, 241
Bse1I ACTGG 1 cut(s) 521
Bse3DI GCAATG 1 cut(s) 423
BseCI ATCGAT 1 cut(s) 674
BseDI CCNNGG 4 cut(s) 174, 283, 299, 624
BseLI CCNNNNNNNGG 4 cut(s) 175, 335, 527, 689
BseMI GCAATG 1 cut(s) 423
BseNI ACTGG 1 cut(s) 521
BseRI GAGGAG 4 cut(s) 224, 315, 318, 489
BseX3I CGGCCG 2 cut(s) 166, 253
BseXI GCAGC 1 cut(s) 21
BseYI CCCAGC 1 cut(s) 511
BsgI GTGCAG 2 cut(s) 369, 422
Bsh1285I CGRYCG 2 cut(s) 169, 256
BshFI GGCC 6 cut(s) 98, 140, 168, 255, 629, 641
BshVI ATCGAT 1 cut(s) 674
BsiEI CGRYCG 2 cut(s) 169, 256
BsiHKAI GWGCWC 1 cut(s) 111
BsiHKCI CYCGRG 1 cut(s) 284
BsiSI CCGG 4 cut(s) 165, 206, 242, 630
BslFI GGGAC 2 cut(s) 543, 632
BslI CCNNNNNNNGG 4 cut(s) 175, 335, 527, 689
BsmAI GTCTC 1 cut(s) 77
BsmFI GGGAC 2 cut(s) 543, 632
BsnI GGCC 6 cut(s) 98, 140, 168, 255, 629, 641
BsoBI CYCGRG 1 cut(s) 284
Bsp1286I GDGCHC 2 cut(s) 111, 292
Bsp143I GATC 3 cut(s) 129, 170, 338
Bsp19I CCATGG 1 cut(s) 624
BspACI CCGC 6 cut(s) 256, 295, 306, 351, 378, 429
BspANI GGCC 6 cut(s) 98, 140, 168, 255, 629, 641
BspDI ATCGAT 1 cut(s) 674
BspLI GGNNCC 1 cut(s) 204
BspMI ACCTGC 4 cut(s) 379, 391, 397, 610
BspPI GGATC 2 cut(s) 165, 346
BsrDI GCAATG 1 cut(s) 423
BsrFI RCCGGY 2 cut(s) 164, 241
BsrI ACTGG 1 cut(s) 521
BssAI RCCGGY 2 cut(s) 164, 241
BssECI CCNNGG 4 cut(s) 174, 283, 299, 624
BssMI GATC 3 cut(s) 129, 170, 338
BssNI GRCGYC 1 cut(s) 234
BssT1I CCWWGG 3 cut(s) 174, 299, 624
Bst4CI ACNGT 3 cut(s) 185, 347, 646
BstACI GRCGYC 1 cut(s) 234
BstAPI GCANNNNNTGC 1 cut(s) 677
BstBAI YACGTR 2 cut(s) 400, 637
BstC8I GCNNGC 2 cut(s) 75, 292
BstDEI CTNAG 1 cut(s) 216
BstDSI CCRYGG 1 cut(s) 624
BstENI CCTNNNNNAGG 2 cut(s) 333, 687
BstKTI GATC 3 cut(s) 132, 173, 341
BstMAI GTCTC 1 cut(s) 77
BstMBI GATC 3 cut(s) 129, 170, 338
BstMCI CGRYCG 2 cut(s) 169, 256
BstMWI GCNNNNNNNGC 2 cut(s) 221, 677
BstPAI GACNNNNGTC 1 cut(s) 56
BstV1I GCAGC 1 cut(s) 21
BstV2I GAAGAC 1 cut(s) 52
BstX2I RGATCY 1 cut(s) 129
BstYI RGATCY 1 cut(s) 129
BstZI CGGCCG 2 cut(s) 166, 253
Bsu15I ATCGAT 1 cut(s) 674
BsuI GTATCC 1 cut(s) 564
BsuRI GGCC 6 cut(s) 98, 140, 168, 255, 629, 641
BsuTUI ATCGAT 1 cut(s) 674
BtgI CCRYGG 1 cut(s) 624
BtsI GCAGTG 1 cut(s) 410
BtsIMutI CAGTG 3 cut(s) 47, 410, 528
BveI ACCTGC 4 cut(s) 379, 391, 397, 610
Cac8I GCNNGC 2 cut(s) 75, 292
Cfr10I RCCGGY 2 cut(s) 164, 241
Cfr13I GGNCC 2 cut(s) 303, 360
ClaI ATCGAT 1 cut(s) 674
CseI GACGC 1 cut(s) 242
Csp6I GTAC 1 cut(s) 121
CspCI CAANNNNNGTGG 2 cut(s) 406, 441
CviAII CATG 4 cut(s) 26, 364, 478, 625
CviQI GTAC 1 cut(s) 121
DdeI CTNAG 1 cut(s) 216
DpnI GATC 3 cut(s) 131, 172, 340
DpnII GATC 3 cut(s) 129, 170, 338
DrdI GACNNNNNNGTC 1 cut(s) 658
DseDI GACNNNNNNGTC 1 cut(s) 658
EaeI YGGCCR 4 cut(s) 166, 253, 627, 639
EagI CGGCCG 2 cut(s) 166, 253
EciI GGCGGA 1 cut(s) 366
EclXI CGGCCG 2 cut(s) 166, 253
Eco130I CCWWGG 3 cut(s) 174, 299, 624
Eco147I AGGCCT 1 cut(s) 98
Eco24I GRGCYC 1 cut(s) 292
Eco47I GGWCC 2 cut(s) 303, 360
Eco52I CGGCCG 2 cut(s) 166, 253
Eco72I CACGTG 2 cut(s) 400, 637
Eco88I CYCGRG 1 cut(s) 284
EcoNI CCTNNNNNAGG 2 cut(s) 333, 687
EcoT14I CCWWGG 3 cut(s) 174, 299, 624
EcoT38I GRGCYC 1 cut(s) 292
ErhI CCWWGG 3 cut(s) 174, 299, 624
FaeI CATG 4 cut(s) 29, 367, 481, 628
FaiI YATR 9 cut(s) 27, 365, 421, 437, 450, 479, 588, 626, 659
FaqI GGGAC 2 cut(s) 543, 632
FatI CATG 4 cut(s) 25, 363, 477, 624
FauI CCCGC 1 cut(s) 385
Fnu4HI GCNGC 4 cut(s) 10, 256, 295, 429
FriOI GRGCYC 1 cut(s) 292
Fsp4HI GCNGC 4 cut(s) 10, 256, 295, 429
FspBI CTAG 1 cut(s) 300
GluI GCNGC 4 cut(s) 10, 256, 295, 429
GsaI CCCAGC 1 cut(s) 515
HaeIII GGCC 6 cut(s) 98, 140, 168, 255, 629, 641
HapII CCGG 4 cut(s) 165, 206, 242, 630
HgaI GACGC 1 cut(s) 242
Hin1I GRCGYC 1 cut(s) 234
Hin1II CATG 4 cut(s) 29, 367, 481, 628
HinfI GANTC 1 cut(s) 85
HpaII CCGG 4 cut(s) 165, 206, 242, 630
HphI GGTGA 5 cut(s) 50, 154, 184, 360, 566
Hpy166II GTNNAC 1 cut(s) 508
Hpy188I TCNGA 2 cut(s) 68, 154
Hpy188III TCNNGA 1 cut(s) 649
Hpy8I GTNNAC 1 cut(s) 508
Hpy99I CGWCG 1 cut(s) 68
HpyAV CCTTC 2 cut(s) 205, 214
HpyCH4III ACNGT 3 cut(s) 185, 347, 646
HpyCH4IV ACGT 3 cut(s) 18, 399, 636
HpyCH4V TGCA 4 cut(s) 270, 386, 392, 403
HpyF10VI GCNNNNNNNGC 2 cut(s) 221, 677
HpyF3I CTNAG 1 cut(s) 216
HpySE526I ACGT 3 cut(s) 18, 399, 636
Hsp92I GRCGYC 1 cut(s) 234
Hsp92II CATG 4 cut(s) 29, 367, 481, 628
Kzo9I GATC 3 cut(s) 129, 170, 338
LmnI GCTCC 6 cut(s) 202, 353, 379, 470, 502, 685
Lsp1109I GCAGC 1 cut(s) 21
LweI GCATC 2 cut(s) 534, 680
MaeI CTAG 1 cut(s) 300
MaeII ACGT 3 cut(s) 18, 399, 636
MaeIII GTNAC 5 cut(s) 19, 179, 190, 366, 440
MalI GATC 3 cut(s) 131, 172, 340
MboI GATC 3 cut(s) 129, 170, 338
MboII GAAGA 1 cut(s) 52
MflI RGATCY 1 cut(s) 129
MhlI GDGCHC 2 cut(s) 111, 292
MlsI TGGCCA 1 cut(s) 641
MluNI TGGCCA 1 cut(s) 641
MlyI GAGTC 1 cut(s) 79
MmeI TCCRAC 2 cut(s) 46, 177
Mox20I TGGCCA 1 cut(s) 641
MscI TGGCCA 1 cut(s) 641
MslI CAYNNNNRTG 1 cut(s) 542
Msp20I TGGCCA 1 cut(s) 641
MspA1I CMGCKG 1 cut(s) 431
MspI CCGG 4 cut(s) 165, 206, 242, 630
MwoI GCNNNNNNNGC 2 cut(s) 221, 677
NcoI CCATGG 1 cut(s) 624
NdeII GATC 3 cut(s) 129, 170, 338
NlaIII CATG 4 cut(s) 29, 367, 481, 628
NlaIV GGNNCC 1 cut(s) 204
NmuCI GTSAC 2 cut(s) 190, 366
PaqCI CACCTGC 3 cut(s) 379, 391, 397
PceI AGGCCT 1 cut(s) 98
PkrI GCNGC 4 cut(s) 11, 257, 296, 430
PleI GAGTC 1 cut(s) 79
PmaCI CACGTG 2 cut(s) 400, 637
PmlI CACGTG 2 cut(s) 400, 637
PpsI GAGTC 1 cut(s) 79
Ppu21I YACGTR 2 cut(s) 400, 637
PshAI GACNNNNGTC 1 cut(s) 56
Psp1406I AACGTT 1 cut(s) 18
PspCI CACGTG 2 cut(s) 400, 637
PspFI CCCAGC 1 cut(s) 511
PspN4I GGNNCC 1 cut(s) 204
PspPI GGNCC 2 cut(s) 303, 360
PsrI GAACNNNNNNTAC 2 cut(s) 473, 505
PsuI RGATCY 1 cut(s) 129
RsaI GTAC 1 cut(s) 122
RsaNI GTAC 1 cut(s) 121
RseI CAYNNNNRTG 1 cut(s) 542
SatI GCNGC 4 cut(s) 10, 256, 295, 429
Sau3AI GATC 3 cut(s) 129, 170, 338
Sau96I GGNCC 2 cut(s) 303, 360
SchI GAGTC 1 cut(s) 79
SduI GDGCHC 2 cut(s) 111, 292
SfaNI GCATC 2 cut(s) 534, 680
SinI GGWCC 2 cut(s) 303, 360
SmiMI CAYNNNNRTG 1 cut(s) 542
SmlI CTYRAG 2 cut(s) 100, 501
SmoI CTYRAG 2 cut(s) 100, 501
SseBI AGGCCT 1 cut(s) 98
SsiI CCGC 6 cut(s) 256, 295, 306, 351, 378, 429
SspMI CTAG 1 cut(s) 300
StuI AGGCCT 1 cut(s) 98
StyI CCWWGG 3 cut(s) 174, 299, 624
TaaI ACNGT 3 cut(s) 185, 347, 646
TaiI ACGT 3 cut(s) 21, 402, 639
TaqI TCGA 3 cut(s) 114, 341, 674
TauI GCSGC 3 cut(s) 258, 297, 431
TscAI CASTG 3 cut(s) 54, 410, 528
TseFI GTSAC 2 cut(s) 190, 366
TseI GCWGC 1 cut(s) 9
Tsp45I GTSAC 2 cut(s) 190, 366
TspDTI ATGAA 1 cut(s) 494
TspRI CASTG 3 cut(s) 54, 410, 528
VpaK11BI GGWCC 2 cut(s) 303, 360
XagI CCTNNNNNAGG 2 cut(s) 333, 687
XmaJI CCTAGG 1 cut(s) 299
XspI CTAG 1 cut(s) 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.