pycom07g20270

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0010198
Physical Location & Seq
Forward (+)
22417154 .. 22418879
1726 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g20270.1

Sequence Viewer

Length: 819 bp
ATGGATCACAACTGTTGTTATGCTGGGACTGGAGGGTTAAGCTGGCCACAGCAGTACAAATCTGGTGCAGTTCCAGCTGCTGCTGCAGTAAGCAATGAATCTTCTTCCAGCTTTCTGGTTCCATGGCCTCCTCCTCCTCCTCATGTGTCGAGCACAAATCAATTCCAGTTTTGTGGGTTGCCGCCTAATTCGTGGTCTTTGACGGCAGCTGACGGTTTTGAAGACGATAGAGATGCATCTGCTTCGAAGAGCCACAGGCAGGCTGAGAAAAGGCGCAGAGATAGAATCAGTGCACAGCTTGCTACTCTTAGGAAACTCGTTCCCAAGTCCGACAAGATGGACAAAGCGGCTCTATTAGGAAGCGTGATCGATCATGTGAAGGAACTAAAGCGAAAAGCGGTGGAAGTGAGCGAAGCCTTCATAGTTCCAACCGAAGTTGATGAAGTAACCATCGACTGTGATCCTGCACATGGTGTAACCTCCACCACTACAAGTAACAGTAACATAAACAGGGGCAATTTTCTCATAACGGCATCCGTTTGCTGCGACGACCGGCCGGACCTCTTCTCGGAGCTCATTCAAATCCTCAAAGGCCTAAAACTCACGGCGCTAAGAGCCGAAATGGCAAGCGTCGGAGGCAGAATCAAAAGCGTTTTGGTGCTTTGTTCTAAGGATGGTGAAGAAGCAGTTTGCTTAAGCACTCTCAAGCAGTCCCTCAAGTTGGTTTTGAGTAAACTTGCCGCGTCGCCAGTGGCGCCAAACTGTCGAATTCGAAGCAAGAGGCAGAGGTTTTTCTTTCCATTCCATGACCCAAACTAA

Protein Analysis

273

Amino Acids

29.57

Weight (kDa)

8.71

Isoelectric Point (pI)

43.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 83 - 130 2.6e-10 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 754
AccII CGCG 1 cut(s) 743
AciI CCGC 4 cut(s) 182, 347, 398, 741
AclWI GGATC 2 cut(s) 12, 455
AcoI YGGCCR 2 cut(s) 44, 554
AcsI RAATTY 1 cut(s) 768
AcyI GRCGYC 1 cut(s) 755
AdeI CACNNNGTG 1 cut(s) 473
AfaI GTAC 1 cut(s) 56
AfiI CCNNNNNNNGG 4 cut(s) 259, 470, 568, 721
AflII CTTAAG 1 cut(s) 694
AgsI TTSAA 2 cut(s) 221, 581
AluBI AGCT 6 cut(s) 42, 77, 111, 209, 298, 574
AluI AGCT 6 cut(s) 42, 77, 111, 209, 298, 574
Alw21I GWGCWC 3 cut(s) 155, 295, 576
Alw44I GTGCAC 1 cut(s) 291
AlwI GGATC 2 cut(s) 12, 455
AlwNI CAGNNNCTG 1 cut(s) 80
AoxI GGCC 4 cut(s) 44, 125, 554, 592
ApaLI GTGCAC 1 cut(s) 291
ApeKI GCWGC 5 cut(s) 77, 80, 83, 206, 543
ApoI RAATTY 1 cut(s) 768
AspLEI GCGC 3 cut(s) 276, 610, 757
AspS9I GGNCC 1 cut(s) 559
AsuHPI GGTGA 1 cut(s) 689
AsuII TTCGAA 2 cut(s) 245, 772
AvaII GGWCC 1 cut(s) 559
BaeGI GKGCMC 1 cut(s) 295
BalI TGGCCA 1 cut(s) 46
BanI GGYRCC 1 cut(s) 754
BanII GRGCYC 1 cut(s) 576
BbsI GAAGAC 1 cut(s) 228
Bbv12I GWGCWC 3 cut(s) 155, 295, 576
BbvI GCAGC 5 cut(s) 64, 67, 70, 218, 530
BccI CCATC 3 cut(s) 331, 458, 668
BceAI ACGGC 3 cut(s) 219, 546, 621
BcgI CGANNNNNNTGC 4 cut(s) 215, 225, 249, 259
BfmI CTRYAG 1 cut(s) 84
BfoI RGCGCY 2 cut(s) 611, 758
BfrI CTTAAG 1 cut(s) 694
BglI GCCNNNNNGGC 1 cut(s) 623
BisI GCNGC 8 cut(s) 78, 81, 84, 182, 207, 348, 544, 741
BlsI GCNGC 8 cut(s) 79, 82, 85, 183, 208, 349, 545, 742
Bme18I GGWCC 1 cut(s) 559
BmgT120I GGNCC 1 cut(s) 559
BmiI GGNNCC 2 cut(s) 120, 756
BmsI GCATC 3 cut(s) 223, 245, 542
BpiI GAAGAC 1 cut(s) 228
BpmI CTGGAG 1 cut(s) 51
Bpu14I TTCGAA 2 cut(s) 245, 772
BpuEI CTTGAG 2 cut(s) 689, 701
Bsa29I ATCGAT 1 cut(s) 369
BsaHI GRCGYC 1 cut(s) 755
BsaJI CCNNGG 1 cut(s) 122
Bsc4I CCNNNNNNNGG 4 cut(s) 259, 470, 568, 721
Bse118I RCCGGY 1 cut(s) 552
Bse1I ACTGG 3 cut(s) 34, 166, 749
Bse3DI GCAATG 1 cut(s) 100
BseCI ATCGAT 1 cut(s) 369
BseDI CCNNGG 1 cut(s) 122
BseGI GGATG 2 cut(s) 533, 679
BseLI CCNNNNNNNGG 4 cut(s) 259, 470, 568, 721
BseMI GCAATG 1 cut(s) 100
BseMII CTCAG 1 cut(s) 255
BseNI ACTGG 3 cut(s) 34, 166, 749
BseRI GAGGAG 4 cut(s) 120, 123, 126, 129
BseSI GKGCMC 1 cut(s) 295
BseX3I CGGCCG 1 cut(s) 554
BseXI GCAGC 5 cut(s) 64, 67, 70, 218, 530
BseYI CCCAGC 1 cut(s) 23
BsgI GTGCAG 2 cut(s) 87, 450
Bsh1236I CGCG 1 cut(s) 743
Bsh1285I CGRYCG 2 cut(s) 553, 557
BshFI GGCC 4 cut(s) 46, 127, 556, 594
BshNI GGYRCC 1 cut(s) 754
BshVI ATCGAT 1 cut(s) 369
BsiEI CGRYCG 2 cut(s) 553, 557
BsiHKAI GWGCWC 3 cut(s) 155, 295, 576
BsiSI CCGG 2 cut(s) 553, 557
BslFI GGGAC 2 cut(s) 40, 697
BslI CCNNNNNNNGG 4 cut(s) 259, 470, 568, 721
BsmFI GGGAC 2 cut(s) 40, 697
BsnI GGCC 4 cut(s) 46, 127, 556, 594
Bsp119I TTCGAA 2 cut(s) 245, 772
Bsp1286I GDGCHC 3 cut(s) 155, 295, 576
Bsp143I GATC 4 cut(s) 4, 366, 370, 460
Bsp19I CCATGG 1 cut(s) 122
BspACI CCGC 4 cut(s) 182, 347, 398, 741
BspANI GGCC 4 cut(s) 46, 127, 556, 594
BspCNI CTCAG 1 cut(s) 256
BspDI ATCGAT 1 cut(s) 369
BspFNI CGCG 1 cut(s) 743
BspLI GGNNCC 2 cut(s) 120, 756
BspMAI CTGCAG 1 cut(s) 88
BspPI GGATC 2 cut(s) 12, 455
BspQI GCTCTTC 1 cut(s) 242
BspT104I TTCGAA 2 cut(s) 245, 772
BspT107I GGYRCC 1 cut(s) 754
BspTI CTTAAG 1 cut(s) 694
BsrDI GCAATG 1 cut(s) 100
BsrFI RCCGGY 1 cut(s) 552
BsrI ACTGG 3 cut(s) 34, 166, 749
BssAI RCCGGY 1 cut(s) 552
BssECI CCNNGG 1 cut(s) 122
BssMI GATC 4 cut(s) 4, 366, 370, 460
BssNI GRCGYC 1 cut(s) 755
BssT1I CCWWGG 1 cut(s) 122
Bst4CI ACNGT 5 cut(s) 14, 215, 458, 500, 764
Bst6I CTCTTC 2 cut(s) 242, 569
BstACI GRCGYC 1 cut(s) 755
BstAFI CTTAAG 1 cut(s) 694
BstAPI GCANNNNNTGC 1 cut(s) 299
BstBI TTCGAA 2 cut(s) 245, 772
BstC8I GCNNGC 4 cut(s) 44, 261, 300, 628
BstDEI CTNAG 4 cut(s) 264, 308, 611, 669
BstDSI CCRYGG 1 cut(s) 122
BstF5I GGATG 2 cut(s) 533, 679
BstFNI CGCG 1 cut(s) 743
BstH2I RGCGCY 2 cut(s) 611, 758
BstHHI GCGC 3 cut(s) 276, 610, 757
BstKTI GATC 4 cut(s) 7, 369, 373, 463
BstMBI GATC 4 cut(s) 4, 366, 370, 460
BstMCI CGRYCG 2 cut(s) 553, 557
BstMWI GCNNNNNNNGC 7 cut(s) 74, 83, 299, 614, 623, 636, 754
BstSFI CTRYAG 1 cut(s) 84
BstSLI GKGCMC 1 cut(s) 295
BstUI CGCG 1 cut(s) 743
BstV1I GCAGC 5 cut(s) 64, 67, 70, 218, 530
BstV2I GAAGAC 1 cut(s) 228
BstXI CCANNNNNNTGG 2 cut(s) 115, 173
BstZI CGGCCG 1 cut(s) 554
Bsu15I ATCGAT 1 cut(s) 369
BsuRI GGCC 4 cut(s) 46, 127, 556, 594
BsuTUI ATCGAT 1 cut(s) 369
BtgI CCRYGG 1 cut(s) 122
BtsCI GGATG 2 cut(s) 533, 679
BtsIMutI CAGTG 2 cut(s) 295, 756
Cac8I GCNNGC 4 cut(s) 44, 261, 300, 628
CaiI CAGNNNCTG 1 cut(s) 80
CfoI GCGC 3 cut(s) 276, 610, 757
Cfr10I RCCGGY 1 cut(s) 552
Cfr13I GGNCC 1 cut(s) 559
ClaI ATCGAT 1 cut(s) 369
CseI GACGC 2 cut(s) 619, 732
Csp6I GTAC 1 cut(s) 55
CviAII CATG 5 cut(s) 123, 143, 374, 470, 806
CviQI GTAC 1 cut(s) 55
DdeI CTNAG 4 cut(s) 264, 308, 611, 669
DinI GGCGCC 1 cut(s) 756
DpnI GATC 4 cut(s) 6, 368, 372, 462
DpnII GATC 4 cut(s) 4, 366, 370, 460
DraIII CACNNNGTG 1 cut(s) 473
EaeI YGGCCR 2 cut(s) 44, 554
EagI CGGCCG 1 cut(s) 554
Eam1104I CTCTTC 2 cut(s) 242, 569
EarI CTCTTC 2 cut(s) 242, 569
Ecl136II GAGCTC 1 cut(s) 574
EclXI CGGCCG 1 cut(s) 554
Eco130I CCWWGG 1 cut(s) 122
Eco147I AGGCCT 1 cut(s) 594
Eco24I GRGCYC 1 cut(s) 576
Eco47I GGWCC 1 cut(s) 559
Eco52I CGGCCG 1 cut(s) 554
Eco53kI GAGCTC 1 cut(s) 574
EcoICRI GAGCTC 1 cut(s) 574
EcoRI GAATTC 1 cut(s) 768
EcoT14I CCWWGG 1 cut(s) 122
EcoT22I ATGCAT 1 cut(s) 238
EcoT38I GRGCYC 1 cut(s) 576
EgeI GGCGCC 1 cut(s) 756
EheI GGCGCC 1 cut(s) 756
ErhI CCWWGG 1 cut(s) 122
FaeI CATG 5 cut(s) 126, 146, 377, 473, 809
FaiI YATR 9 cut(s) 21, 124, 144, 375, 422, 471, 506, 527, 807
FaqI GGGAC 2 cut(s) 40, 697
FatI CATG 5 cut(s) 122, 142, 373, 469, 805
Fnu4HI GCNGC 8 cut(s) 78, 81, 84, 182, 207, 348, 544, 741
FokI GGATG 2 cut(s) 520, 686
FriOI GRGCYC 1 cut(s) 576
Fsp4HI GCNGC 8 cut(s) 78, 81, 84, 182, 207, 348, 544, 741
GlaI GCGC 3 cut(s) 275, 609, 756
GluI GCNGC 8 cut(s) 78, 81, 84, 182, 207, 348, 544, 741
GsaI CCCAGC 1 cut(s) 27
GsuI CTGGAG 1 cut(s) 51
HaeII RGCGCY 2 cut(s) 611, 758
HaeIII GGCC 4 cut(s) 46, 127, 556, 594
HapII CCGG 2 cut(s) 553, 557
HgaI GACGC 2 cut(s) 619, 732
HhaI GCGC 3 cut(s) 276, 610, 757
Hin1I GRCGYC 1 cut(s) 755
Hin1II CATG 5 cut(s) 126, 146, 377, 473, 809
Hin6I GCGC 3 cut(s) 274, 608, 755
HinP1I GCGC 3 cut(s) 274, 608, 755
HinfI GANTC 3 cut(s) 98, 285, 642
HpaII CCGG 2 cut(s) 553, 557
HphI GGTGA 1 cut(s) 689
Hpy166II GTNNAC 2 cut(s) 293, 734
Hpy188I TCNGA 3 cut(s) 331, 571, 635
Hpy8I GTNNAC 2 cut(s) 293, 734
Hpy99I CGWCG 3 cut(s) 551, 635, 748
HpyAV CCTTC 2 cut(s) 373, 427
HpyCH4III ACNGT 5 cut(s) 14, 215, 458, 500, 764
HpyCH4V TGCA 5 cut(s) 68, 86, 236, 293, 467
HpyF10VI GCNNNNNNNGC 7 cut(s) 74, 83, 299, 614, 623, 636, 754
HpyF3I CTNAG 4 cut(s) 264, 308, 611, 669
Hsp92I GRCGYC 1 cut(s) 755
Hsp92II CATG 5 cut(s) 126, 146, 377, 473, 809
HspAI GCGC 3 cut(s) 274, 608, 755
KasI GGCGCC 1 cut(s) 754
Kzo9I GATC 4 cut(s) 4, 366, 370, 460
LguI GCTCTTC 1 cut(s) 242
LmnI GCTCC 1 cut(s) 571
Lsp1109I GCAGC 5 cut(s) 64, 67, 70, 218, 530
LweI GCATC 3 cut(s) 223, 245, 542
MaeIII GTNAC 4 cut(s) 445, 475, 494, 500
MalI GATC 4 cut(s) 6, 368, 372, 462
MboI GATC 4 cut(s) 4, 366, 370, 460
MboII GAAGA 6 cut(s) 93, 96, 233, 259, 556, 692
MhlI GDGCHC 3 cut(s) 155, 295, 576
MlsI TGGCCA 1 cut(s) 46
MluCI AATT 4 cut(s) 161, 187, 517, 768
MluNI TGGCCA 1 cut(s) 46
Mly113I GGCGCC 1 cut(s) 755
MmeI TCCRAC 3 cut(s) 354, 452, 613
Mox20I TGGCCA 1 cut(s) 46
Mph1103I ATGCAT 1 cut(s) 238
MscI TGGCCA 1 cut(s) 46
MseI TTAA 2 cut(s) 38, 695
Msp20I TGGCCA 1 cut(s) 46
MspA1I CMGCKG 2 cut(s) 77, 209
MspCI CTTAAG 1 cut(s) 694
MspI CCGG 2 cut(s) 553, 557
MvnI CGCG 1 cut(s) 743
MwoI GCNNNNNNNGC 7 cut(s) 74, 83, 299, 614, 623, 636, 754
NarI GGCGCC 1 cut(s) 755
NcoI CCATGG 1 cut(s) 122
NdeII GATC 4 cut(s) 4, 366, 370, 460
NlaIII CATG 5 cut(s) 126, 146, 377, 473, 809
NlaIV GGNNCC 2 cut(s) 120, 756
NsiI ATGCAT 1 cut(s) 238
NspV TTCGAA 2 cut(s) 245, 772
PceI AGGCCT 1 cut(s) 594
PciSI GCTCTTC 1 cut(s) 242
PfeI GAWTC 3 cut(s) 98, 285, 642
PkrI GCNGC 8 cut(s) 79, 82, 85, 183, 208, 349, 545, 742
PluTI GGCGCC 1 cut(s) 758
Psp124BI GAGCTC 1 cut(s) 576
PspFI CCCAGC 1 cut(s) 23
PspN4I GGNNCC 2 cut(s) 120, 756
PspPI GGNCC 1 cut(s) 559
PstI CTGCAG 1 cut(s) 88
PstNI CAGNNNCTG 1 cut(s) 80
PvuII CAGCTG 2 cut(s) 77, 209
RsaI GTAC 1 cut(s) 56
RsaNI GTAC 1 cut(s) 55
SacI GAGCTC 1 cut(s) 576
SapI GCTCTTC 1 cut(s) 242
SaqAI TTAA 2 cut(s) 38, 695
SatI GCNGC 8 cut(s) 78, 81, 84, 182, 207, 348, 544, 741
Sau3AI GATC 4 cut(s) 4, 366, 370, 460
Sau96I GGNCC 1 cut(s) 559
SduI GDGCHC 3 cut(s) 155, 295, 576
SetI ASST 9 cut(s) 44, 79, 113, 211, 300, 482, 564, 576, 791
SfaNI GCATC 3 cut(s) 223, 245, 542
SfcI CTRYAG 1 cut(s) 84
SfoI GGCGCC 1 cut(s) 756
SfuI TTCGAA 2 cut(s) 245, 772
SinI GGWCC 1 cut(s) 559
SmlI CTYRAG 3 cut(s) 694, 704, 716
SmoI CTYRAG 3 cut(s) 694, 704, 716
Sse9I AATT 4 cut(s) 161, 187, 517, 768
SseBI AGGCCT 1 cut(s) 594
SsiI CCGC 4 cut(s) 182, 347, 398, 741
SspDI GGCGCC 1 cut(s) 754
SstI GAGCTC 1 cut(s) 576
StuI AGGCCT 1 cut(s) 594
StyI CCWWGG 1 cut(s) 122
TaaI ACNGT 5 cut(s) 14, 215, 458, 500, 764
TaqI TCGA 6 cut(s) 149, 245, 369, 453, 766, 772
TasI AATT 4 cut(s) 161, 187, 517, 768
TatI WGTACW 1 cut(s) 54
TauI GCSGC 3 cut(s) 184, 350, 743
TfiI GAWTC 3 cut(s) 98, 285, 642
Tru1I TTAA 2 cut(s) 38, 695
Tru9I TTAA 2 cut(s) 38, 695
TscAI CASTG 2 cut(s) 295, 756
TseI GCWGC 5 cut(s) 77, 80, 83, 206, 543
TspDTI ATGAA 3 cut(s) 111, 409, 456
TspGWI ACGGA 1 cut(s) 526
TspRI CASTG 2 cut(s) 295, 756
Vha464I CTTAAG 1 cut(s) 694
VneI GTGCAC 1 cut(s) 291
VpaK11BI GGWCC 1 cut(s) 559
XapI RAATTY 1 cut(s) 768
Zsp2I ATGCAT 1 cut(s) 238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.