pycom07g20410

Mad3/BUB1 hoMad3/BUB1 homology region 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0010450
Physical Location & Seq
Forward (+)
22513988 .. 22516731
2744 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1620 bp
ATGTTAAAACCTCCCTCCTCCTCCTCCACCTCCACCACCACTACCACCATGTCGAACAACGATCTTTTCACCTCCCTAATCTCCGACGTCAAAACCTACTCCGGCAAAGACCCTCTTCTCCCATGGCTAAGGTACGCTCTCGCCACCTCCATCTCTTCCTCGCTATCAGTTACCATTCTACTGTTTAACCCATTCGATTTCACGTTGTGGTTGCAGAGGGATTCGAAAAATGAACGACTCACTCCCGCCTCATACTCTGAAGGAGAAACTTCCTCGCTTCTTGCAGAAGTGTGCGCAGACGTTCGAATGGATTTTGTCGATGATCCGAGAGCTCTGTTGAGAACAATGGAGGCGAATCAAATCGGGACAAAGCACTCTTTGTTCTACCAGGCATATGCTCTCTACTATGAAAAGAACAAGAAATTTGAGGAAGCTGAGAAGATGTACCACTTGGGAGTGCAAAACCTTGCAGAGCCCATTGGAGCGTTAGAGAAGTCGTACGAGCAGTTCCTTCAACGAATGGAGAGACACAGGAGACACAGAAACCAGGAAAAGAGGACCTCAAAAAGGCCTCTGTCAGATAGGACTATCCCTCCAAATGGAGAAAGGAACAAAGAAAATGCCTGCAGGATCGGCCCCAGGCCTATAGAATCCCAACATGATCCGTGTAGAAGTGAGCATCAAGTGACTTCTGGAAGAGGGTCAGAGGAACCGAAAATGTTTGGCGGCGATGATACAGTTGTGGTGAAGTTTGTAGACACTGCCATTGTTGGAAAGTCTGAAGCGGAAGATGCGTGTCACCATGGATTGGTGGATCCTACTATAAACATGAAAGAGGCCATGAGCGCCATCAATGACATGTTTCGAGAGCCTATAGAGACGGCTCCCATTAGCAGAAGATCTCGACAATCCCAACCGAAGAACAATACTGTGGACAGTGGATTCCAAGTATTTGTTGATGACAACTTAGATAATGGAGGGAAATCAGAAAATAATAAGGGAGATTTGAGTAGAACTGCTACCCACCAGTCTCACCAAGCACCTTTAAACATTTTCATTGATGATGATGATAATGACGACAAAGATAGCCCTGATCAGAGTGACGTCGGAAATCCGACAGATGATTCTACCTCACCTGCATCACATGGAAATGTATTCGTGTTTCCAAATCCAAAAGATTTTCCGGGTGAAAGTTCCGGTGATTTGGATGTTGGAGGCTCATCTCGACCGAGGTTCAGGGAGGATACAATGGTTTGTAGGTTTGTTGGGGCCACCATTTCGGATGAGCCGGAGGAGGTGGAAAATGCCTGCCACCATGGTTTGGTTGACCCTACAATCAACCTAAAGGAAGCCATGGATGATATCAACAACATGTTTGGGAAGCCAATGGAATTTGTAAGAAAGAGGAGAGTGAAAAAGCAGGAAAGAGGACTTGATAGGAAAGAAGATTTTGGTGGGTTTTCTATACTTCCTGATGATAACTTGAACCAGAAGCAAGAGCAGAAAATGCCTGATTTACCGCAGAGATCAAGAGATTCAGATTTGTTCGAGGCCACTGTAACAACAAAGGAGGCCATGGATGAGATCAATAAGATGTTTGGAATGCCCCTGGACTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mad3_BUB1_I

Protein Analysis

540

Amino Acids

60.7

Weight (kDa)

5.1

Isoelectric Point (pI)

55.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mad3_BUB1_I PF08311 103 - 153 2.4e-12 Mad3/BUB1 homology region 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010450)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G05510
fragaria_vesca FvH4_7g24900 FvH4_7g24900 FvH4_7g24900 FvH4_7g24900 FvH4_7g24900
malus_domestica MD01G1156700.v1.1 MD07G1225400.v1.1
prunus_persica Prupe.2G254200_v2.0.a1
pyrus_communis pycom01g17350 pycom07g20410
rosa_chinensis RchiOBHm_Chr1g0370781
rosa_laevigata RLG00000027010
rosa_multiflora Rmu_sc0009474.1_g000004
rosa_roxburghii Rroxscaffold_4G00286230
rosa_rugosa Rorug01G0359700
rosa_samantha Rh1AG368800 Rh1BG332400 Rh1CG346300 Rh1DG363800
rosa_wichuraiana Rw1G032470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1144
AatII GACGTC 2 cut(s) 90, 1107
Acc16I TGCGCA 1 cut(s) 295
Acc36I ACCTGC 1 cut(s) 1144
AccB7I CCANNNNNTGG 2 cut(s) 808, 1321
AccI GTMKAC 1 cut(s) 756
AciI CCGC 4 cut(s) 246, 726, 785, 1520
AclWI GGATC 5 cut(s) 317, 638, 656, 809, 822
AcsI RAATTY 2 cut(s) 422, 1391
AcuI CTGAAG 2 cut(s) 279, 801
AcyI GRCGYC 2 cut(s) 87, 1104
AdeI CACNNNGTG 1 cut(s) 207
AfaI GTAC 3 cut(s) 134, 446, 500
AfiI CCNNNNNNNGG 4 cut(s) 567, 599, 808, 1321
AflIII ACRYGT 2 cut(s) 858, 1371
AgsI TTSAA 2 cut(s) 515, 1486
AjnI CCWGG 4 cut(s) 387, 546, 638, 1608
AluBI AGCT 2 cut(s) 332, 434
AluI AGCT 2 cut(s) 332, 434
Alw21I GWGCWC 1 cut(s) 334
Alw26I GTCTC 4 cut(s) 520, 529, 872, 1035
AlwI GGATC 5 cut(s) 317, 638, 656, 809, 822
AoxI GGCC 7 cut(s) 569, 634, 641, 837, 1269, 1551, 1572
ApoI RAATTY 2 cut(s) 422, 1391
AspLEI GCGC 2 cut(s) 296, 848
AspS9I GGNCC 3 cut(s) 558, 635, 1269
AsuC2I CCSGG 1 cut(s) 1185
AsuHPI GGTGA 7 cut(s) 61, 757, 791, 1025, 1125, 1199, 1211
AsuII TTCGAA 2 cut(s) 224, 304
AvaII GGWCC 1 cut(s) 558
BaeI ACNNNNGTAYC 4 cut(s) 726, 759, 1236, 1269
BamHI GGATCC 1 cut(s) 814
BanII GRGCYC 2 cut(s) 334, 477
Bbv12I GWGCWC 1 cut(s) 334
BccI CCATC 2 cut(s) 158, 857
BceAI ACGGC 1 cut(s) 897
BciT130I CCWGG 4 cut(s) 389, 548, 640, 1610
BciVI GTATCC 1 cut(s) 1237
BclI TGATCA 1 cut(s) 1093
BcnI CCSGG 1 cut(s) 1185
BcoDI GTCTC 4 cut(s) 520, 529, 872, 1035
BfaI CTAG 1 cut(s) 1618
BfmI CTRYAG 3 cut(s) 625, 645, 873
BfoI RGCGCY 1 cut(s) 849
BfuAI ACCTGC 1 cut(s) 1144
BfuI GTATCC 1 cut(s) 1237
BglII AGATCT 1 cut(s) 899
BisI GCNGC 1 cut(s) 727
BlsI GCNGC 1 cut(s) 728
Bme1390I CCNGG 5 cut(s) 389, 548, 640, 1185, 1610
Bme18I GGWCC 1 cut(s) 558
BmgT120I GGNCC 3 cut(s) 558, 635, 1269
BmiI GGNNCC 5 cut(s) 637, 711, 816, 885, 1270
BmrFI CCNGG 5 cut(s) 389, 548, 640, 1185, 1610
BmsI GCATC 3 cut(s) 688, 781, 1148
Bpu10I CCTNAGC 1 cut(s) 128
Bpu14I TTCGAA 2 cut(s) 224, 304
BpuMI CCSGG 1 cut(s) 1185
BsaHI GRCGYC 2 cut(s) 87, 1104
BsaJI CCNNGG 8 cut(s) 122, 638, 802, 1229, 1315, 1353, 1575, 1608
BsaWI WCCGGW 1 cut(s) 1196
BsaXI ACNNNNNCTCC 2 cut(s) 577, 607
Bsc4I CCNNNNNNNGG 4 cut(s) 567, 599, 808, 1321
Bse1I ACTGG 1 cut(s) 1027
BseBI CCWGG 4 cut(s) 389, 548, 640, 1610
BseDI CCNNGG 8 cut(s) 122, 638, 802, 1229, 1315, 1353, 1575, 1608
BseGI GGATG 4 cut(s) 1213, 1288, 1363, 1585
BseLI CCNNNNNNNGG 4 cut(s) 567, 599, 808, 1321
BseMII CTCAG 1 cut(s) 426
BseNI ACTGG 1 cut(s) 1027
BseRI GAGGAG 5 cut(s) 7, 10, 13, 1307, 1420
Bsh1285I CGRYCG 1 cut(s) 1229
BshFI GGCC 7 cut(s) 571, 636, 643, 839, 1271, 1553, 1574
BsiEI CGRYCG 1 cut(s) 1229
BsiHKAI GWGCWC 1 cut(s) 334
BsiSI CCGG 4 cut(s) 102, 1184, 1197, 1289
BsiWI CGTACG 1 cut(s) 498
BslFI GGGAC 1 cut(s) 379
BslI CCNNNNNNNGG 4 cut(s) 567, 599, 808, 1321
BsmAI GTCTC 4 cut(s) 520, 529, 872, 1035
BsmBI CGTCTC 1 cut(s) 872
BsmFI GGGAC 1 cut(s) 379
BsmI GAATGC 1 cut(s) 1608
BsnI GGCC 7 cut(s) 571, 636, 643, 839, 1271, 1553, 1574
Bsp119I TTCGAA 2 cut(s) 224, 304
Bsp1286I GDGCHC 2 cut(s) 334, 477
Bsp143I GATC 9 cut(s) 61, 322, 630, 661, 814, 899, 1093, 1526, 1584
Bsp19I CCATGG 5 cut(s) 122, 802, 1315, 1353, 1575
BspACI CCGC 4 cut(s) 246, 726, 785, 1520
BspANI GGCC 7 cut(s) 571, 636, 643, 839, 1271, 1553, 1574
BspCNI CTCAG 1 cut(s) 427
BspLI GGNNCC 5 cut(s) 637, 711, 816, 885, 1270
BspMAI CTGCAG 1 cut(s) 629
BspMI ACCTGC 1 cut(s) 1144
BspPI GGATC 5 cut(s) 317, 638, 656, 809, 822
BspT104I TTCGAA 2 cut(s) 224, 304
BsrI ACTGG 1 cut(s) 1027
BssECI CCNNGG 8 cut(s) 122, 638, 802, 1229, 1315, 1353, 1575, 1608
BssMI GATC 9 cut(s) 61, 322, 630, 661, 814, 899, 1093, 1526, 1584
BssNI GRCGYC 2 cut(s) 87, 1104
BssT1I CCWWGG 5 cut(s) 122, 802, 1315, 1353, 1575
Bst2UI CCWGG 4 cut(s) 389, 548, 640, 1610
Bst4CI ACNGT 5 cut(s) 183, 739, 931, 938, 1558
Bst6I CTCTTC 3 cut(s) 120, 160, 691
BstACI GRCGYC 2 cut(s) 87, 1104
BstAPI GCANNNNNTGC 1 cut(s) 1507
BstBI TTCGAA 2 cut(s) 224, 304
BstC8I GCNNGC 2 cut(s) 625, 1309
BstDEI CTNAG 3 cut(s) 128, 435, 967
BstDSI CCRYGG 5 cut(s) 122, 802, 1315, 1353, 1575
BstENI CCTNNNNNAGG 1 cut(s) 565
BstF5I GGATG 4 cut(s) 1213, 1288, 1363, 1585
BstH2I RGCGCY 1 cut(s) 849
BstHHI GCGC 2 cut(s) 296, 848
BstKTI GATC 9 cut(s) 64, 325, 633, 664, 817, 902, 1096, 1529, 1587
BstMAI GTCTC 4 cut(s) 520, 529, 872, 1035
BstMBI GATC 9 cut(s) 61, 322, 630, 661, 814, 899, 1093, 1526, 1584
BstMCI CGRYCG 1 cut(s) 1229
BstMWI GCNNNNNNNGC 4 cut(s) 633, 791, 845, 1507
BstNI CCWGG 4 cut(s) 389, 548, 640, 1610
BstNSI RCATGY 2 cut(s) 862, 1375
BstSCI CCNGG 5 cut(s) 387, 546, 638, 1183, 1608
BstSFI CTRYAG 3 cut(s) 625, 645, 873
BstX2I RGATCY 2 cut(s) 814, 899
BstYI RGATCY 2 cut(s) 814, 899
BsuI GTATCC 1 cut(s) 1237
BsuRI GGCC 7 cut(s) 571, 636, 643, 839, 1271, 1553, 1574
BtgI CCRYGG 5 cut(s) 122, 802, 1315, 1353, 1575
BtgZI GCGATG 1 cut(s) 744
BtsCI GGATG 4 cut(s) 1213, 1288, 1363, 1585
BtsI GCAGTG 1 cut(s) 759
BtsIMutI CAGTG 3 cut(s) 759, 943, 1554
BveI ACCTGC 1 cut(s) 1144
Cac8I GCNNGC 2 cut(s) 625, 1309
CfoI GCGC 2 cut(s) 296, 848
Cfr13I GGNCC 3 cut(s) 558, 635, 1269
Csp6I GTAC 3 cut(s) 133, 445, 499
CviQI GTAC 3 cut(s) 133, 445, 499
DdeI CTNAG 3 cut(s) 128, 435, 967
DpnI GATC 9 cut(s) 63, 324, 632, 663, 816, 901, 1095, 1528, 1586
DpnII GATC 9 cut(s) 61, 322, 630, 661, 814, 899, 1093, 1526, 1584
DraI TTTAAA 1 cut(s) 1047
DraIII CACNNNGTG 1 cut(s) 207
Eam1104I CTCTTC 3 cut(s) 120, 160, 691
EarI CTCTTC 3 cut(s) 120, 160, 691
Ecl136II GAGCTC 1 cut(s) 332
Eco130I CCWWGG 5 cut(s) 122, 802, 1315, 1353, 1575
Eco147I AGGCCT 2 cut(s) 571, 643
Eco24I GRGCYC 2 cut(s) 334, 477
Eco32I GATATC 1 cut(s) 1363
Eco47I GGWCC 1 cut(s) 558
Eco53kI GAGCTC 1 cut(s) 332
Eco57I CTGAAG 2 cut(s) 279, 801
EcoICRI GAGCTC 1 cut(s) 332
EcoNI CCTNNNNNAGG 1 cut(s) 565
EcoO109I RGGNCCY 1 cut(s) 558
EcoRII CCWGG 4 cut(s) 387, 546, 638, 1608
EcoRV GATATC 1 cut(s) 1363
EcoT14I CCWWGG 5 cut(s) 122, 802, 1315, 1353, 1575
EcoT38I GRGCYC 2 cut(s) 334, 477
ErhI CCWWGG 5 cut(s) 122, 802, 1315, 1353, 1575
Esp3I CGTCTC 1 cut(s) 872
FalI AAGNNNNNCTT 2 cut(s) 99, 131
FaqI GGGAC 1 cut(s) 379
FauI CCCGC 1 cut(s) 253
FauNDI CATATG 1 cut(s) 394
FbaI TGATCA 1 cut(s) 1093
FblI GTMKAC 1 cut(s) 756
Fnu4HI GCNGC 1 cut(s) 727
FokI GGATG 4 cut(s) 1220, 1295, 1370, 1592
FriOI GRGCYC 2 cut(s) 334, 477
Fsp4HI GCNGC 1 cut(s) 727
FspBI CTAG 1 cut(s) 1618
FspI TGCGCA 1 cut(s) 295
GlaI GCGC 2 cut(s) 295, 847
GluI GCNGC 1 cut(s) 727
HaeII RGCGCY 1 cut(s) 849
HaeIII GGCC 7 cut(s) 571, 636, 643, 839, 1271, 1553, 1574
HapII CCGG 4 cut(s) 102, 1184, 1197, 1289
HhaI GCGC 2 cut(s) 296, 848
Hin1I GRCGYC 2 cut(s) 87, 1104
Hin6I GCGC 2 cut(s) 294, 846
HinP1I GCGC 2 cut(s) 294, 846
HincII GTYRAC 1 cut(s) 1327
HindII GTYRAC 1 cut(s) 1327
HinfI GANTC 7 cut(s) 221, 237, 355, 650, 942, 1124, 1535
HpaII CCGG 4 cut(s) 102, 1184, 1197, 1289
HphI GGTGA 7 cut(s) 61, 757, 791, 1025, 1125, 1199, 1211
Hpy166II GTNNAC 3 cut(s) 757, 934, 1327
Hpy188III TCNNGA 7 cut(s) 364, 693, 866, 903, 1224, 1472, 1530
Hpy8I GTNNAC 3 cut(s) 757, 934, 1327
Hpy99I CGWCG 2 cut(s) 89, 1109
HpyAV CCTTC 2 cut(s) 254, 521
HpyCH4III ACNGT 5 cut(s) 183, 739, 931, 938, 1558
HpyCH4IV ACGT 4 cut(s) 87, 203, 300, 1104
HpyCH4V TGCA 6 cut(s) 214, 284, 460, 470, 627, 1139
HpyF10VI GCNNNNNNNGC 4 cut(s) 633, 791, 845, 1507
HpyF3I CTNAG 3 cut(s) 128, 435, 967
HpySE526I ACGT 4 cut(s) 87, 203, 300, 1104
Hsp92I GRCGYC 2 cut(s) 87, 1104
HspAI GCGC 2 cut(s) 294, 846
Ksp22I TGATCA 1 cut(s) 1093
Kzo9I GATC 9 cut(s) 61, 322, 630, 661, 814, 899, 1093, 1526, 1584
LmnI GCTCC 2 cut(s) 482, 889
LweI GCATC 3 cut(s) 688, 781, 1148
MaeI CTAG 1 cut(s) 1618
MaeII ACGT 4 cut(s) 87, 203, 300, 1104
MaeIII GTNAC 5 cut(s) 169, 685, 797, 1100, 1558
MalI GATC 9 cut(s) 63, 324, 632, 663, 816, 901, 1095, 1528, 1586
MboI GATC 9 cut(s) 61, 322, 630, 661, 814, 899, 1093, 1526, 1584
MboII GAAGA 8 cut(s) 107, 147, 451, 708, 800, 909, 931, 1457
MflI RGATCY 2 cut(s) 814, 899
MhlI GDGCHC 2 cut(s) 334, 477
MluCI AATT 2 cut(s) 422, 1391
MlyI GAGTC 1 cut(s) 231
MmeI TCCRAC 5 cut(s) 108, 751, 1087, 1139, 1192
MseI TTAA 3 cut(s) 5, 186, 1046
MslI CAYNNNNRTG 1 cut(s) 1149
MspI CCGG 4 cut(s) 102, 1184, 1197, 1289
MspR9I CCNGG 5 cut(s) 389, 548, 640, 1185, 1610
Mva1269I GAATGC 1 cut(s) 1608
MvaI CCWGG 4 cut(s) 389, 548, 640, 1610
MwoI GCNNNNNNNGC 4 cut(s) 633, 791, 845, 1507
NciI CCSGG 1 cut(s) 1185
NcoI CCATGG 5 cut(s) 122, 802, 1315, 1353, 1575
NdeI CATATG 1 cut(s) 394
NdeII GATC 9 cut(s) 61, 322, 630, 661, 814, 899, 1093, 1526, 1584
NlaIV GGNNCC 5 cut(s) 637, 711, 816, 885, 1270
NmuCI GTSAC 3 cut(s) 685, 797, 1100
NsbI TGCGCA 1 cut(s) 295
NspI RCATGY 2 cut(s) 862, 1375
NspV TTCGAA 2 cut(s) 224, 304
PaqCI CACCTGC 1 cut(s) 1144
PceI AGGCCT 2 cut(s) 571, 643
PciI ACATGT 2 cut(s) 858, 1371
PctI GAATGC 1 cut(s) 1608
PfeI GAWTC 6 cut(s) 221, 355, 650, 942, 1124, 1535
Pfl23II CGTACG 1 cut(s) 498
PflMI CCANNNNNTGG 2 cut(s) 808, 1321
PkrI GCNGC 1 cut(s) 728
PleI GAGTC 1 cut(s) 231
PpsI GAGTC 1 cut(s) 231
PpuMI RGGWCCY 1 cut(s) 558
PscI ACATGT 2 cut(s) 858, 1371
Psp124BI GAGCTC 1 cut(s) 334
Psp5II RGGWCCY 1 cut(s) 558
Psp6I CCWGG 4 cut(s) 387, 546, 638, 1608
PspGI CCWGG 4 cut(s) 387, 546, 638, 1608
PspLI CGTACG 1 cut(s) 498
PspN4I GGNNCC 5 cut(s) 637, 711, 816, 885, 1270
PspPI GGNCC 3 cut(s) 558, 635, 1269
PspPPI RGGWCCY 1 cut(s) 558
PsrI GAACNNNNNNTAC 2 cut(s) 491, 523
PstI CTGCAG 1 cut(s) 629
PsuI RGATCY 2 cut(s) 814, 899
RsaI GTAC 3 cut(s) 134, 446, 500
RsaNI GTAC 3 cut(s) 133, 445, 499
RseI CAYNNNNRTG 1 cut(s) 1149
SacI GAGCTC 1 cut(s) 334
SaqAI TTAA 3 cut(s) 5, 186, 1046
SatI GCNGC 1 cut(s) 727
Sau3AI GATC 9 cut(s) 61, 322, 630, 661, 814, 899, 1093, 1526, 1584
Sau96I GGNCC 3 cut(s) 558, 635, 1269
SbfI CCTGCAGG 1 cut(s) 629
SchI GAGTC 1 cut(s) 231
ScrFI CCNGG 5 cut(s) 389, 548, 640, 1185, 1610
SdaI CCTGCAGG 1 cut(s) 629
SduI GDGCHC 2 cut(s) 334, 477
SfaNI GCATC 3 cut(s) 688, 781, 1148
SfcI CTRYAG 3 cut(s) 625, 645, 873
SfuI TTCGAA 2 cut(s) 224, 304
SinI GGWCC 1 cut(s) 558
SmiMI CAYNNNNRTG 1 cut(s) 1149
Sse8387I CCTGCAGG 1 cut(s) 629
Sse9I AATT 2 cut(s) 422, 1391
SseBI AGGCCT 2 cut(s) 571, 643
SsiI CCGC 4 cut(s) 246, 726, 785, 1520
SspMI CTAG 1 cut(s) 1618
SstI GAGCTC 1 cut(s) 334
StuI AGGCCT 2 cut(s) 571, 643
StyD4I CCNGG 5 cut(s) 387, 546, 638, 1183, 1608
StyI CCWWGG 5 cut(s) 122, 802, 1315, 1353, 1575
TaaI ACNGT 5 cut(s) 183, 739, 931, 938, 1558
TaiI ACGT 4 cut(s) 90, 206, 303, 1107
TaqI TCGA 9 cut(s) 53, 195, 224, 304, 318, 865, 904, 1225, 1548
TaqII GACCGA 1 cut(s) 1243
TasI AATT 2 cut(s) 422, 1391
TauI GCSGC 1 cut(s) 729
TfiI GAWTC 6 cut(s) 221, 355, 650, 942, 1124, 1535
Tru1I TTAA 3 cut(s) 5, 186, 1046
Tru9I TTAA 3 cut(s) 5, 186, 1046
TscAI CASTG 3 cut(s) 766, 943, 1561
TseFI GTSAC 3 cut(s) 685, 797, 1100
Tsp45I GTSAC 3 cut(s) 685, 797, 1100
TspDTI ATGAA 4 cut(s) 246, 423, 845, 1045
TspGWI ACGGA 1 cut(s) 654
TspRI CASTG 3 cut(s) 766, 943, 1561
Van91I CCANNNNNTGG 2 cut(s) 808, 1321
VpaK11BI GGWCC 1 cut(s) 558
XagI CCTNNNNNAGG 1 cut(s) 565
XapI RAATTY 2 cut(s) 422, 1391
XceI RCATGY 2 cut(s) 862, 1375
XmiI GTMKAC 1 cut(s) 756
XspI CTAG 1 cut(s) 1618
ZraI GACGTC 2 cut(s) 88, 1105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.