pycom07g20580

DNA repair and recombination protein RAD54-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0005309
Physical Location & Seq
Reverse (-)
22610535 .. 22615948
5414 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g20580.2

Sequence Viewer

Length: 3858 bp
ATGAGGATGAAGAGGAAAAGACATCTGTATCGGGCTAAGCATCCGTTCGATGCTAATCCTTTTGAGGCTCTTTGCTGTGGTTCGTGGCAACCTGTGGAAGTTCTGAGCATCAGGCGTGGTAAGATGACCATGAGCTTCGGAAACAATCATCCCTGTATAATCAGGGACAAAGGTCCATTCGCGAACCTACGAGTCAGGTCCAGACATGCAAATATGTATGATTGCACCTGCTTTCTGAGGCCGGGTGTTGATATTTGTGTGCTGTCAACCCCTGAACATACAGATGATTCAGAAGAAGGCAGTCGGGATCCAGTGATGGTTGATGCTCGAATAAGCTCGATCGAAAGGGTGCCGCATGAGTCTGATCGGTGCTCATGCCAATTCTATGTGAACTTTTATGTGAACCAAGGACCTCTTGGTACCGAGAGAGCAACACTGAACAAGGAAGCCAAACTGGTTGGAATTGATGAAATTTTCGTCTTCCAAATGATTGACCGCGATGCTTATGAGAATCAACCCTACCGTTGGGAGTGCTCTGCGGACTGCCCAACGCTACCAAGAACGAAATTGCTCTTGGGGACGTTCTTATCCGATGTTTCATGGTTACTTGTGGCGTCCGTGATGAAGCGGGTTATATTTGATATCAGATCAGTGAAGACCAAAATCGTGTACCAGATTTTGGAAGGCGACAATGACCGAACTCTGTTACATTCTGACAACTTCTTGCATGCCGTAAATTTCAAAGTAGACAATGGGCTCCTCGTACCTATGGTAGTCGAATTTGTTCCAGATGATGCTACCGAGGCTGATCCTACTCCTGAAATGGATGAAAGCGTACCCTCACCATCTACTGAGCTTGTTGGCCTGCGTCGTTCCAAGCGTCGAATTATACAACCTGAGCGTTTCCTTGCCTGTGATGCTCCATCAGAGATAGAGATTGGCTATGTTCGAAGCAGGCCATATAAGGTAGATTACAGCTCAGAAGATGATGAATTGTATATACCGTTATCACAGCTGTTTGCCAAACATGCAAGTCATTCAGAAGAGCACACCGAGGCAGAACAAAAGTTCCGTCCTAAGAAGTTGAAGTCCAGTAAGGATGACAATTATTGGAAAACTGAGGAAGAACTTGCTTGCAAAAGCGATGATGATGACGACGTTGAATACAAAATCAAGACCAAGTCGAAGAAGGTGAAATCAGGTGCGGGTAAGCAGAAAAGACATCGAGATGAACTTGCCCTTGTTGAATACAGAAGAGACCCTTTAACCCTTGGACATGGTCATCGGAGTGTCAACAGTACTCCTGAAAAGGGTGCAAACGAGAGCGATGAGTTTCCTGCGAAGTACTATTACCATCACAATCGTAAAACAAAGAGAAAGAATAATTCTGACTTAGATGATATGGACTTCCAAATGAAATGGGATGGAAAAGTGTCGACTAGCAGAGCTAGCAGAGTTTTATACAGAAGGCATCACTCTATTCGTTCGAAAAGGGACCCTAACGGAAGAACTTACCCAAGGAGGAGCTTAAATACTGGTGCATACAAGGAACTGATAAATTCATTCTTGAAAGACATGGACTGCACAAATAAGCCAGACGCAAATATTATGGACCAGTGGAACGAATTCAAGGCCGCAAAGAACTCTGACCAACGGAAGGAAACTGAAATGCCAGAAAATGACGATGAAGAGGAGGAAATGTCCGAGACTGAGATGCTGTGGAAAGAAATGGAATTGGCATTGGCATCAGCTTACCTTCAAGATAATGAGGGATCAAATGCTGCTGCGGCAAATGCAAGTCCACCAAAGTCAGACTCAGCTTGCCGACATGAATTTAAATTAAACGAAGAAATTGGGATAATATGCAGCATATGTGGTTTTGTCAGCACTGAAATAAGAGATGTATCTCCCCCCTTTGTGCAAAATGCCAGTGTGGACACATATGATAGAAAGAGTAATGTAGAAGAACCCGACAATAAGCGAGCTGAGTACGAAGAATTCAATTTCTTCCACACTCGTACTACTCCGGATGATCCTGAGACTTTGTCGGAAGAAAGTGACAATGTTTGGGCCCTAATTCCTGAACTCAGAAAGAAATTATTATTCCACCAGAAAAAGGCCTTTGAGTTTCTATGGAAAAATGTTGCAGGATCTTTGGAACCGTCACTCATGGAACGAAAAGCCAAGAAAACCGGTGGTTGCGTTATATCTCATACCCCTGGAGCCGGGAAGACTTTTCTAATCATTGCATTTCTTACTAGCTACCTCAAGTTATTCCCGGGGAAACGGCCTCTCGTCCTTGCTCCCAAGACAACACTCTATACCTGGTACAAAGAATTTATCAAATGGCAGATTCCTATACCTGTGTATCTGATTCACGGTCGAAGAACCTACAGAGTGTTCAAGAAGAAGACCATAAACTTTACAGGAGGCCCGAAGCCAACGGATGATGTGATGCATGTGTTGGATTGCCTGGAAAAAATACAGAAGTGGCATGCACAGCCTAGTGTTCTTGTCATGGGTTATACCTCATTCCTGACGCTGATGCGCGAAGACTCGAAGTTTGTGCACAGAAAATTTATGGCGCAAGTGCTGCGTGAGAGTCCAGGTCTTGTAGTTCTAGATGAAGGGCACAATCCGAGAAGTACTAAATCCAGGCTGAGGAAGGGACTGATGAAAGTTGAAACGGACTTGAGAATATTGCTTTCGGGAACTCTTTTTCAGAACAACTTCTGCGAGTACTTCAACACCCTCTGCTTGGCAAGGCCAAAGTTTGTGAATGAAGTTTTAAGGGCGTTGGACCCAAAATACAGAAGGATAAAGAAAGGGAAGGAAAAAGCGCGCCATTTGATGGAAGCCCGAGCACGAAAGTTGTTCTTGGATAAGATTGCCAAGAAGATTGATTCCAATGAAGAAGAAGATCAGAGGATCCAAGGTCTAAATCAATTGAGGCATATAACAAATGGGTTCATTGATGTGTATGAAGGTGGAAATTCAGACACCCTTCCTGGTCTACAGATTTACACCTTGTTGATGAACACTACCGACATACAACGAGATATGTTGGACAGACTGCAAAACATAATGGCTAACTACAAGGGGTATCCTCTGGAACTTGAACTTCTGATAACCCTTGGATCGATACATCCATGGTTAATCACGACTGCTTGCTGCGCCGGGAAGTTTTTTCGTCCTGAGGAACTGATGGAGCTTGAGCAGTACAAGTATGATCTGCACAAAGGGTCAAAAGTCAAGTTTGTTTTGAACCTTGTTTACCAAGTTGTCAGGAATGAGAAGGTTTTGATTTTCTGTCACAACATTGCACCGGTGAGGCTATTTCTCGAACTGTTTGAAAGGGTGTTCGGGTGGCAGAGAGGCAGAGAAGTTTTGGTTCTCACAGGGGATTTGGAGCTTTTCGACCGAGGAAAGGTGATGGACAAATTTGAAGAGCCCGGCGGAGCCTCGAGGGTCCTGCTTGCATCGATCACAGCTTGCGCCGAGGGAATCAGCTTGACAGCAGCTTCTCGGGTCATCTTGTTGGACTCCGAATGGAACCCTTCTAAGACAAAGCAGGCGATTGCGCGCGCATTTCGGCCTGGACAGCAGAAGGTGGTGTATGTGTATCAGCTCTTGGCAACCGGAACTCTTGAAGAAGACAAGTATGGCAGGACAACTTGGAAGGAGTGGGTGTCAAGCATGATTTTCAGTGAGGCATTCGTGGAGGACCCGTCACGCTGGCAGGCAGAGAAGATCGAAGACGATGTGCTGAGAGAGATGGTGGCAGAGGACAAGAGCAAATCGTTCCATATGATCATGAAAAATGAAAAGGCTTCAACTGTGGTGAGAGGTAAGGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000018 GO:0000280 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0006139 GO:0006259 GO:0006310 GO:0006311 GO:0006325 GO:0006338 GO:0006342 GO:0006355 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007127 GO:0007131 GO:0008094 GO:0008150 GO:0008152 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0015616 GO:0016043 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0022402 GO:0022414 GO:0030491 GO:0030702 GO:0031047 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0033170 GO:0034641 GO:0035822 GO:0035825 GO:0035861 GO:0040029 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051052 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051321 GO:0060255 GO:0061806 GO:0061982 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090734 GO:0097159 GO:0140013 GO:0140097 GO:1901360 GO:1901363 GO:1902679 GO:1903046 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03440 map03440
Pfam Domains
Protein Families
Helicase_C Methyltransf_11 Methyltransf_21 SNF2_N

Protein Analysis

1286

Amino Acids

148.24

Weight (kDa)

8.09

Isoelectric Point (pI)

45.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 236
AbsI CCTCGAGG 1 cut(s) 3463
Acc36I ACCTGC 1 cut(s) 236
Acc65I GGTACC 1 cut(s) 419
AccB1I GGYRCC 2 cut(s) 349, 419
AccB7I CCANNNNNTGG 2 cut(s) 679, 2851
AccI GTMKAC 3 cut(s) 747, 1436, 3013
AccII CGCG 6 cut(s) 182, 498, 2550, 2842, 3583, 3585
AccIII TCCGGA 1 cut(s) 2026
AciI CCGC 8 cut(s) 353, 496, 539, 628, 1205, 1635, 1787, 3456
AclWI GGATC 9 cut(s) 302, 315, 803, 1780, 2027, 2158, 2923, 2936, 3145
AcyI GRCGYC 1 cut(s) 614
AgeI ACCGGT 2 cut(s) 2192, 3325
AjnI CCWGG 7 cut(s) 2218, 2324, 2472, 2605, 2654, 3007, 3595
AjuI GAANNNNNNNTTGG 2 cut(s) 557, 589
AloI GAACNNNNNNTCC 2 cut(s) 1053, 1085
Alw21I GWGCWC 5 cut(s) 374, 536, 1050, 2571, 2866
Alw26I GTCTC 3 cut(s) 1251, 1700, 2033
Alw44I GTGCAC 1 cut(s) 2567
AlwI GGATC 9 cut(s) 302, 315, 803, 1780, 2027, 2158, 2923, 2936, 3145
Ama87I CYCGRG 4 cut(s) 2278, 2859, 3463, 3525
Aor13HI TCCGGA 1 cut(s) 2026
ApaI GGGCCC 1 cut(s) 2074
ApaLI GTGCAC 1 cut(s) 2567
ApeKI GCWGC 6 cut(s) 1781, 1784, 1866, 2593, 3171, 3518
ArsI GACNNNNNNTTYG 4 cut(s) 1057, 1089, 2546, 2578
AsiGI ACCGGT 2 cut(s) 2192, 3325
Asp700I GAANNNNTTC 3 cut(s) 783, 1625, 2729
Asp718I GGTACC 1 cut(s) 419
AspLEI GCGC 9 cut(s) 2550, 2587, 2842, 2844, 3176, 3497, 3583, 3585, 3587
AsuC2I CCSGG 6 cut(s) 243, 2227, 2279, 2280, 3178, 3453
AsuHPI GGTGA 5 cut(s) 834, 1204, 3340, 3442, 3853
AsuII TTCGAA 2 cut(s) 949, 1487
AsuNHI GCTAGC 1 cut(s) 1448
AvaI CYCGRG 4 cut(s) 2278, 2859, 3463, 3525
AvaII GGWCC 8 cut(s) 173, 198, 410, 1495, 1612, 2800, 3469, 3724
AxyI CCTNAGG 1 cut(s) 3195
BaeGI GKGCMC 3 cut(s) 2074, 2571, 2634
BaeI ACNNNNGTAYC 4 cut(s) 11, 44, 2351, 2384
BamHI GGATCC 2 cut(s) 307, 2928
BanI GGYRCC 2 cut(s) 349, 419
BanII GRGCYC 3 cut(s) 759, 2074, 3453
BarI GAAGNNNNNNTAC 2 cut(s) 1334, 1366
BbsI GAAGAC 7 cut(s) 472, 662, 2237, 2417, 2559, 3660, 3762
Bbv12I GWGCWC 5 cut(s) 374, 536, 1050, 2571, 2866
BbvCI CCTCAGC 1 cut(s) 2660
BbvI GCAGC 6 cut(s) 1768, 1771, 1878, 2580, 3158, 3530
BccI CCATC 9 cut(s) 310, 853, 931, 1362, 1418, 2845, 3199, 3427, 3769
BceAI ACGGC 2 cut(s) 716, 2303
BcgI CGANNNNNNTGC 4 cut(s) 2548, 2582, 3454, 3488
BciT130I CCWGG 7 cut(s) 2220, 2326, 2474, 2607, 2656, 3009, 3597
BciVI GTATCC 1 cut(s) 3114
BclI TGATCA 1 cut(s) 3810
BcnI CCSGG 6 cut(s) 243, 2227, 2279, 2280, 3178, 3453
BcoDI GTCTC 3 cut(s) 1251, 1700, 2033
BfaI CTAG 5 cut(s) 1440, 1449, 2259, 2505, 2621
BfmI CTRYAG 2 cut(s) 2392, 3014
BfuAI ACCTGC 1 cut(s) 236
BfuI GTATCC 1 cut(s) 3114
BisI GCNGC 9 cut(s) 353, 1635, 1782, 1785, 1788, 1867, 2594, 3172, 3519
BlpI GCTNAGC 1 cut(s) 36
BlsI GCNGC 9 cut(s) 354, 1636, 1783, 1786, 1789, 1868, 2595, 3173, 3520
BmcAI AGTACT 4 cut(s) 1300, 1346, 2647, 2741
Bme18I GGWCC 8 cut(s) 173, 198, 410, 1495, 1612, 2800, 3469, 3724
BmeT110I CYCGRG 4 cut(s) 2278, 2859, 3463, 3525
BmtI GCTAGC 1 cut(s) 1452
BoxI GACNNNNGTC 1 cut(s) 171
BpiI GAAGAC 7 cut(s) 472, 662, 2237, 2417, 2559, 3660, 3762
BpmI CTGGAG 1 cut(s) 2241
Bpu10I CCTNAGC 2 cut(s) 897, 2660
Bpu1102I GCTNAGC 1 cut(s) 36
Bpu14I TTCGAA 2 cut(s) 949, 1487
BpuEI CTTGAG 3 cut(s) 2252, 2713, 3233
BpuMI CCSGG 6 cut(s) 243, 2227, 2279, 2280, 3178, 3453
Bsa29I ATCGAT 2 cut(s) 3140, 3482
BsaBI GATNNNNATC 1 cut(s) 54
BsaHI GRCGYC 1 cut(s) 614
BsaI GGTCTC 1 cut(s) 1251
BsaWI WCCGGW 4 cut(s) 2026, 2192, 3325, 3638
BsaXI ACNNNNNCTCC 6 cut(s) 1685, 1715, 3674, 3704, 3713, 3743
Bse118I RCCGGY 2 cut(s) 2192, 3325
Bse1I ACTGG 6 cut(s) 311, 459, 1092, 1540, 1615, 1929
Bse21I CCTNAGG 1 cut(s) 3195
Bse3DI GCAATG 2 cut(s) 2244, 3318
Bse8I GATNNNNATC 1 cut(s) 54
BseAI TCCGGA 1 cut(s) 2026
BseBI CCWGG 7 cut(s) 2220, 2326, 2474, 2607, 2656, 3009, 3597
BseCI ATCGAT 2 cut(s) 3140, 3482
BseGI GGATG 9 cut(s) 12, 40, 148, 832, 1105, 1429, 2035, 2452, 3145
BseJI GATNNNNATC 1 cut(s) 54
BseMI GCAATG 2 cut(s) 2244, 3318
BseNI ACTGG 6 cut(s) 311, 459, 1092, 1540, 1615, 1929
BsePI GCGCGC 3 cut(s) 2840, 3581, 3583
BseRI GAGGAG 3 cut(s) 749, 1537, 1706
BseSI GKGCMC 3 cut(s) 2074, 2571, 2634
BseXI GCAGC 6 cut(s) 1768, 1771, 1878, 2580, 3158, 3530
BsgI GTGCAG 2 cut(s) 1567, 3218
Bsh1236I CGCG 6 cut(s) 182, 498, 2550, 2842, 3583, 3585
Bsh1285I CGRYCG 3 cut(s) 342, 2383, 3421
BshNI GGYRCC 2 cut(s) 349, 419
BshTI ACCGGT 2 cut(s) 2192, 3325
BshVI ATCGAT 2 cut(s) 3140, 3482
BsiEI CGRYCG 3 cut(s) 342, 2383, 3421
BsiHKAI GWGCWC 5 cut(s) 374, 536, 1050, 2571, 2866
BsiHKCI CYCGRG 4 cut(s) 2278, 2859, 3463, 3525
BsiSI CCGG 9 cut(s) 242, 2027, 2193, 2226, 2279, 3177, 3326, 3453, 3639
BslFI GGGAC 4 cut(s) 179, 592, 1508, 2682
BsmAI GTCTC 3 cut(s) 1251, 1700, 2033
BsmFI GGGAC 4 cut(s) 179, 592, 1508, 2682
BsmI GAATGC 1 cut(s) 3713
Bso31I GGTCTC 1 cut(s) 1251
BsoBI CYCGRG 4 cut(s) 2278, 2859, 3463, 3525
Bsp119I TTCGAA 2 cut(s) 949, 1487
Bsp120I GGGCCC 1 cut(s) 2070
Bsp1286I GDGCHC 9 cut(s) 374, 536, 759, 1050, 2074, 2571, 2634, 2866, 3453
Bsp13I TCCGGA 1 cut(s) 2026
Bsp1720I GCTNAGC 1 cut(s) 36
Bsp19I CCATGG 1 cut(s) 3149
Bsp68I TCGCGA 1 cut(s) 182
BspACI CCGC 8 cut(s) 353, 496, 539, 628, 1205, 1635, 1787, 3456
BspDI ATCGAT 2 cut(s) 3140, 3482
BspEI TCCGGA 1 cut(s) 2026
BspFNI CGCG 6 cut(s) 182, 498, 2550, 2842, 3583, 3585
BspHI TCATGA 1 cut(s) 3813
BspMI ACCTGC 1 cut(s) 236
BspOI GCTAGC 1 cut(s) 1452
BspPI GGATC 9 cut(s) 302, 315, 803, 1780, 2027, 2158, 2923, 2936, 3145
BspQI GCTCTTC 2 cut(s) 1038, 3441
BspT104I TTCGAA 2 cut(s) 949, 1487
BspT107I GGYRCC 2 cut(s) 349, 419
BspTNI GGTCTC 1 cut(s) 1251
BsrDI GCAATG 2 cut(s) 2244, 3318
BsrFI RCCGGY 2 cut(s) 2192, 3325
BsrI ACTGG 6 cut(s) 311, 459, 1092, 1540, 1615, 1929
BssAI RCCGGY 2 cut(s) 2192, 3325
BssHII GCGCGC 3 cut(s) 2840, 3581, 3583
BssNI GRCGYC 1 cut(s) 614
BssT1I CCWWGG 6 cut(s) 406, 1270, 1517, 2932, 3133, 3149
Bst2UI CCWGG 7 cut(s) 2220, 2326, 2474, 2607, 2656, 3009, 3597
Bst4CI ACNGT 7 cut(s) 524, 1005, 1298, 2163, 2381, 3348, 3838
Bst6I CTCTTC 5 cut(s) 5, 1038, 1249, 1683, 3441
BstACI GRCGYC 1 cut(s) 614
BstAPI GCANNNNNTGC 1 cut(s) 2593
BstBI TTCGAA 2 cut(s) 949, 1487
BstDSI CCRYGG 1 cut(s) 3149
BstF5I GGATG 9 cut(s) 12, 40, 148, 832, 1105, 1429, 2035, 2452, 3145
BstFNI CGCG 6 cut(s) 182, 498, 2550, 2842, 3583, 3585
BstHHI GCGC 9 cut(s) 2550, 2587, 2842, 2844, 3176, 3497, 3583, 3585, 3587
BstMAI GTCTC 3 cut(s) 1251, 1700, 2033
BstMCI CGRYCG 3 cut(s) 342, 2383, 3421
BstNI CCWGG 7 cut(s) 2220, 2326, 2474, 2607, 2656, 3009, 3597
BstNSI RCATGY 5 cut(s) 209, 731, 1031, 2462, 2498
BstPAI GACNNNNGTC 1 cut(s) 171
BstSFI CTRYAG 2 cut(s) 2392, 3014
BstSLI GKGCMC 3 cut(s) 2074, 2571, 2634
BstUI CGCG 6 cut(s) 182, 498, 2550, 2842, 3583, 3585
BstV1I GCAGC 6 cut(s) 1768, 1771, 1878, 2580, 3158, 3530
BstV2I GAAGAC 7 cut(s) 472, 662, 2237, 2417, 2559, 3660, 3762
BstX2I RGATCY 3 cut(s) 307, 2150, 2928
BstYI RGATCY 3 cut(s) 307, 2150, 2928
Bsu15I ATCGAT 2 cut(s) 3140, 3482
Bsu36I CCTNAGG 1 cut(s) 3195
BsuI GTATCC 1 cut(s) 3114
BsuTUI ATCGAT 2 cut(s) 3140, 3482
BtgI CCRYGG 1 cut(s) 3149
BtgZI GCGATG 3 cut(s) 513, 1158, 1341
BtsCI GGATG 9 cut(s) 12, 40, 148, 832, 1105, 1429, 2035, 2452, 3145
BtsIMutI CAGTG 7 cut(s) 318, 434, 657, 1622, 1887, 1936, 3712
BtuMI TCGCGA 1 cut(s) 182
BveI ACCTGC 1 cut(s) 236
CciI TCATGA 1 cut(s) 3813
CfoI GCGC 9 cut(s) 2550, 2587, 2842, 2844, 3176, 3497, 3583, 3585, 3587
Cfr10I RCCGGY 2 cut(s) 2192, 3325
Cfr9I CCCGGG 1 cut(s) 2278
ClaI ATCGAT 2 cut(s) 3140, 3482
CseI GACGC 5 cut(s) 603, 857, 869, 1607, 2548
CsiI ACCWGGT 1 cut(s) 2324
CspAI ACCGGT 2 cut(s) 2192, 3325
DraI TTTAAA 1 cut(s) 1837
Eam1104I CTCTTC 5 cut(s) 5, 1038, 1249, 1683, 3441
EarI CTCTTC 5 cut(s) 5, 1038, 1249, 1683, 3441
EciI GGCGGA 1 cut(s) 3471
Eco130I CCWWGG 6 cut(s) 406, 1270, 1517, 2932, 3133, 3149
Eco147I AGGCCT 1 cut(s) 2120
Eco24I GRGCYC 3 cut(s) 759, 2074, 3453
Eco31I GGTCTC 1 cut(s) 1251
Eco32I GATATC 1 cut(s) 643
Eco47I GGWCC 8 cut(s) 173, 198, 410, 1495, 1612, 2800, 3469, 3724
Eco81I CCTNAGG 1 cut(s) 3195
Eco88I CYCGRG 4 cut(s) 2278, 2859, 3463, 3525
EcoO109I RGGNCCY 5 cut(s) 410, 1495, 2071, 3469, 3724
EcoRI GAATTC 2 cut(s) 1625, 1997
EcoRII CCWGG 7 cut(s) 2218, 2324, 2472, 2605, 2654, 3007, 3595
EcoRV GATATC 1 cut(s) 643
EcoT14I CCWWGG 6 cut(s) 406, 1270, 1517, 2932, 3133, 3149
EcoT22I ATGCAT 1 cut(s) 2460
EcoT38I GRGCYC 3 cut(s) 759, 2074, 3453
ErhI CCWWGG 6 cut(s) 406, 1270, 1517, 2932, 3133, 3149
FalI AAGNNNNNCTT 6 cut(s) 399, 431, 1246, 1278, 2861, 2893
FaqI GGGAC 4 cut(s) 179, 592, 1508, 2682
FauI CCCGC 2 cut(s) 621, 1198
FauNDI CATATG 3 cut(s) 1871, 1942, 3807
FbaI TGATCA 1 cut(s) 3810
FblI GTMKAC 3 cut(s) 747, 1436, 3013
Fnu4HI GCNGC 9 cut(s) 353, 1635, 1782, 1785, 1788, 1867, 2594, 3172, 3519
FokI GGATG 9 cut(s) 19, 27, 135, 839, 1112, 1436, 2042, 2459, 3132
FriOI GRGCYC 3 cut(s) 759, 2074, 3453
Fsp4HI GCNGC 9 cut(s) 353, 1635, 1782, 1785, 1788, 1867, 2594, 3172, 3519
FspBI CTAG 5 cut(s) 1440, 1449, 2259, 2505, 2621
GlaI GCGC 9 cut(s) 2549, 2586, 2841, 2843, 3175, 3496, 3582, 3584, 3586
GluI GCNGC 9 cut(s) 353, 1635, 1782, 1785, 1788, 1867, 2594, 3172, 3519
GsuI CTGGAG 1 cut(s) 2241
HapII CCGG 9 cut(s) 242, 2027, 2193, 2226, 2279, 3177, 3326, 3453, 3639
HgaI GACGC 5 cut(s) 603, 857, 869, 1607, 2548
HhaI GCGC 9 cut(s) 2550, 2587, 2842, 2844, 3176, 3497, 3583, 3585, 3587
Hin1I GRCGYC 1 cut(s) 614
Hin6I GCGC 9 cut(s) 2548, 2585, 2840, 2842, 3174, 3495, 3581, 3583, 3585
HinP1I GCGC 9 cut(s) 2548, 2585, 2840, 2842, 3174, 3495, 3581, 3583, 3585
HincII GTYRAC 3 cut(s) 267, 1294, 1437
HindII GTYRAC 3 cut(s) 267, 1294, 1437
HpaII CCGG 9 cut(s) 242, 2027, 2193, 2226, 2279, 3177, 3326, 3453, 3639
HphI GGTGA 5 cut(s) 834, 1204, 3340, 3442, 3853
Hpy99I CGWCG 3 cut(s) 873, 885, 1160
HpyCH4III ACNGT 7 cut(s) 524, 1005, 1298, 2163, 2381, 3348, 3838
HpyCH4IV ACGT 2 cut(s) 581, 1158
HpySE526I ACGT 2 cut(s) 581, 1158
Hsp92I GRCGYC 1 cut(s) 614
HspAI GCGC 9 cut(s) 2548, 2585, 2840, 2842, 3174, 3495, 3581, 3583, 3585
KflI GGGWCCC 1 cut(s) 1495
Kpn2I TCCGGA 1 cut(s) 2026
KpnI GGTACC 1 cut(s) 423
Ksp22I TGATCA 1 cut(s) 3810
LguI GCTCTTC 2 cut(s) 1038, 3441
LmnI GCTCC 8 cut(s) 762, 925, 1524, 2222, 2308, 3208, 3409, 3458
Lsp1109I GCAGC 6 cut(s) 1768, 1771, 1878, 2580, 3158, 3530
MabI ACCWGGT 1 cut(s) 2324
MaeI CTAG 5 cut(s) 1440, 1449, 2259, 2505, 2621
MaeII ACGT 2 cut(s) 581, 1158
MaeIII GTNAC 6 cut(s) 603, 705, 2057, 2163, 3311, 3729
MfeI CAATTG 1 cut(s) 2945
MflI RGATCY 3 cut(s) 307, 2150, 2928
MhlI GDGCHC 9 cut(s) 374, 536, 759, 1050, 2074, 2571, 2634, 2866, 3453
MlyI GAGTC 6 cut(s) 201, 368, 1808, 2549, 2611, 3536
MmeI TCCRAC 6 cut(s) 439, 2028, 2445, 2778, 3045, 3519
Mph1103I ATGCAT 1 cut(s) 2460
MroI TCCGGA 1 cut(s) 2026
MroXI GAANNNNTTC 3 cut(s) 783, 1625, 2729
MseI TTAA 6 cut(s) 1265, 1529, 1836, 1841, 2789, 3155
MslI CAYNNNNRTG 4 cut(s) 282, 1227, 1287, 2975
MspA1I CMGCKG 1 cut(s) 1015
MspI CCGG 9 cut(s) 242, 2027, 2193, 2226, 2279, 3177, 3326, 3453, 3639
MunI CAATTG 1 cut(s) 2945
Mva1269I GAATGC 1 cut(s) 3713
MvaI CCWGG 7 cut(s) 2220, 2326, 2474, 2607, 2656, 3009, 3597
MvnI CGCG 6 cut(s) 182, 498, 2550, 2842, 3583, 3585
NciI CCSGG 6 cut(s) 243, 2227, 2279, 2280, 3178, 3453
NcoI CCATGG 1 cut(s) 3149
NdeI CATATG 3 cut(s) 1871, 1942, 3807
NheI GCTAGC 1 cut(s) 1448
NmeAIII GCCGAG 1 cut(s) 3523
NmuCI GTSAC 4 cut(s) 2057, 2163, 3311, 3729
NruI TCGCGA 1 cut(s) 182
NsiI ATGCAT 1 cut(s) 2460
NspI RCATGY 5 cut(s) 209, 731, 1031, 2462, 2498
NspV TTCGAA 2 cut(s) 949, 1487
PaeI GCATGC 2 cut(s) 731, 2498
PaeR7I CTCGAG 1 cut(s) 3463
PagI TCATGA 1 cut(s) 3813
PaqCI CACCTGC 1 cut(s) 236
PauI GCGCGC 3 cut(s) 2840, 3581, 3583
PceI AGGCCT 1 cut(s) 2120
PciSI GCTCTTC 2 cut(s) 1038, 3441
PcsI WCGNNNNNNNCGW 1 cut(s) 877
PctI GAATGC 1 cut(s) 3713
PdmI GAANNNNTTC 3 cut(s) 783, 1625, 2729
PfeI GAWTC 6 cut(s) 287, 511, 2353, 2374, 2903, 3504
PflFI GACNNNGTC 3 cut(s) 1180, 1278, 2044
PflMI CCANNNNNTGG 2 cut(s) 679, 2851
PinAI ACCGGT 2 cut(s) 2192, 3325
PkrI GCNGC 9 cut(s) 354, 1636, 1783, 1786, 1789, 1868, 2595, 3173, 3520
Ple19I CGATCG 1 cut(s) 342
PleI GAGTC 6 cut(s) 200, 367, 1808, 2549, 2610, 3536
PpsI GAGTC 6 cut(s) 200, 367, 1808, 2549, 2610, 3536
PpuMI RGGWCCY 4 cut(s) 410, 1495, 3469, 3724
PshAI GACNNNNGTC 1 cut(s) 171
Psp5II RGGWCCY 4 cut(s) 410, 1495, 3469, 3724
Psp6I CCWGG 7 cut(s) 2218, 2324, 2472, 2605, 2654, 3007, 3595
PspGI CCWGG 7 cut(s) 2218, 2324, 2472, 2605, 2654, 3007, 3595
PspOMI GGGCCC 1 cut(s) 2070
PspPPI RGGWCCY 4 cut(s) 410, 1495, 3469, 3724
PspXI VCTCGAGB 1 cut(s) 3463
PsrI GAACNNNNNNTAC 2 cut(s) 3257, 3289
PsuI RGATCY 3 cut(s) 307, 2150, 2928
PsyI GACNNNGTC 3 cut(s) 1180, 1278, 2044
PteI GCGCGC 3 cut(s) 2840, 3581, 3583
PvuI CGATCG 1 cut(s) 342
PvuII CAGCTG 1 cut(s) 1015
RruI TCGCGA 1 cut(s) 182
RseI CAYNNNNRTG 4 cut(s) 282, 1227, 1287, 2975
SalI GTCGAC 1 cut(s) 1435
SapI GCTCTTC 2 cut(s) 1038, 3441
SaqAI TTAA 6 cut(s) 1265, 1529, 1836, 1841, 2789, 3155
SatI GCNGC 9 cut(s) 353, 1635, 1782, 1785, 1788, 1867, 2594, 3172, 3519
ScaI AGTACT 4 cut(s) 1300, 1346, 2647, 2741
SchI GAGTC 6 cut(s) 201, 368, 1808, 2549, 2611, 3536
SduI GDGCHC 9 cut(s) 374, 536, 759, 1050, 2074, 2571, 2634, 2866, 3453
SexAI ACCWGGT 1 cut(s) 2324
SfcI CTRYAG 2 cut(s) 2392, 3014
Sfr274I CTCGAG 1 cut(s) 3463
SfuI TTCGAA 2 cut(s) 949, 1487
SgrAI CRCCGGYG 1 cut(s) 3325
SinI GGWCC 8 cut(s) 173, 198, 410, 1495, 1612, 2800, 3469, 3724
SlaI CTCGAG 1 cut(s) 3463
SmaI CCCGGG 1 cut(s) 2280
SmiI ATTTAAAT 1 cut(s) 1837
SmiMI CAYNNNNRTG 4 cut(s) 282, 1227, 1287, 2975
SmlI CTYRAG 4 cut(s) 2267, 2692, 3212, 3463
SmoI CTYRAG 4 cut(s) 2267, 2692, 3212, 3463
SphI GCATGC 2 cut(s) 731, 2498
SseBI AGGCCT 1 cut(s) 2120
SsiI CCGC 8 cut(s) 353, 496, 539, 628, 1205, 1635, 1787, 3456
SspI AATATT 2 cut(s) 1606, 2700
SspMI CTAG 5 cut(s) 1440, 1449, 2259, 2505, 2621
StuI AGGCCT 1 cut(s) 2120
StyI CCWWGG 6 cut(s) 406, 1270, 1517, 2932, 3133, 3149
SwaI ATTTAAAT 1 cut(s) 1837
TaaI ACNGT 7 cut(s) 524, 1005, 1298, 2163, 2381, 3348, 3838
TaiI ACGT 2 cut(s) 584, 1161
TaqII GACCGA 2 cut(s) 711, 3435
TatI WGTACW 5 cut(s) 1298, 1344, 2645, 2739, 3219
TauI GCSGC 3 cut(s) 355, 1637, 1790
TfiI GAWTC 6 cut(s) 287, 511, 2353, 2374, 2903, 3504
Tru1I TTAA 6 cut(s) 1265, 1529, 1836, 1841, 2789, 3155
Tru9I TTAA 6 cut(s) 1265, 1529, 1836, 1841, 2789, 3155
TscAI CASTG 7 cut(s) 318, 441, 657, 1622, 1894, 1936, 3712
TseFI GTSAC 4 cut(s) 2057, 2163, 3311, 3729
TseI GCWGC 6 cut(s) 1781, 1784, 1866, 2593, 3171, 3518
Tsp45I GTSAC 4 cut(s) 2057, 2163, 3311, 3729
TspGWI ACGGA 7 cut(s) 33, 607, 1061, 1518, 1669, 2459, 2702
TspMI CCCGGG 1 cut(s) 2278
TspRI CASTG 7 cut(s) 318, 441, 657, 1622, 1894, 1936, 3712
Tth111I GACNNNGTC 3 cut(s) 1180, 1278, 2044
Van91I CCANNNNNTGG 2 cut(s) 679, 2851
VneI GTGCAC 1 cut(s) 2567
VpaK11BI GGWCC 8 cut(s) 173, 198, 410, 1495, 1612, 2800, 3469, 3724
XbaI TCTAGA 1 cut(s) 2620
XceI RCATGY 5 cut(s) 209, 731, 1031, 2462, 2498
XcmI CCANNNNNNNNNTGG 1 cut(s) 413
XhoI CTCGAG 1 cut(s) 3463
XmaI CCCGGG 1 cut(s) 2278
XmiI GTMKAC 3 cut(s) 747, 1436, 3013
XmnI GAANNNNTTC 3 cut(s) 783, 1625, 2729
XspI CTAG 5 cut(s) 1440, 1449, 2259, 2505, 2621
ZrmI AGTACT 4 cut(s) 1300, 1346, 2647, 2741
Zsp2I ATGCAT 1 cut(s) 2460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.