Rorug01G0361500

DNA repair and recombination protein RAD54-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0005309
Physical Location & Seq
Forward (+)
47699635 .. 47703536
3902 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0361500.1

Sequence Viewer

Length: 2364 bp
ATGTACCATTCTTTGTTAAGGCAAGGTCTAGAGCCAGACAAGTATACCTTTAACTTTGCCCTGAAGGCTTGCACTGCTGCATTGGATTTTGAAGAGGGTGTATTGGTGCACCGTGAAGTAGAGCGTAAGGGGTTAGAGTATGATGTGTTTATTGGGACCAGTCTGATTGATATGTATTGTAAAATGGGGGAGGTGAAATGTGCAGGAGAGGTGTTTGATAGAATGCCTGAGAAAGATGTTGTGGCATGTAATGCTTTGATTGCCGGGTTAGCGAAAAGCGAGGATCCTAATGAAGCAGTGGAGTTATTTAGGAGGATGCAATGTTGGGGGATGGGGGTGAATTTAGTGAGCTTGTTTAACTTGGTTCCAGCAGTGTCTAGATTGGGAGATATTGATTCTTGTAGGTGTATTCATGGGTATGTGGTTAGGAGGGAGTTGGGTTTAGAGGTTTCAAATGGGTTGGTTGATATGTATTGCAAGTGTGGAGATGTGGATGTTGCTCGCAGGGTTTTCGAGATGGTGCAGGGTGGTGATGATGATGGTGGTGTTTTGTGGGGGACAATGATGGCGGGGTACGCGAGTAATGGATGTTTTGTTGAGGTTTTGGAGTTGTTTGATTGCTTGAAAGGGGAGGGGAGTAGAATGAATAAGGTTTGTATCATGAGTGCACTTTTGGCTGCTACTGAGATGAGAGATTTGGAGAAAGGGAGGGAGATCCATCTTTGTGCTTTAGAAGTAGAAGTCGATTCTGATGTTTCGGTTGCTACTTCTATAATGACCATGTATGCAAAATGTGGAGAAATAGAGAAGGCAATGCAATTGTTTGAGAGACTTGGGGAAAGAGATTTAGTTGCTTGGTCTGCACTTATATCTGCTTGCGTCCAGTCTGGTTATCCTGAAGTGGCATTGTCTCTCTTTCGAGATAGGCAGAATGAAAATCTCAAACCATGTGGCATAACATGGGTCAGCGTCCTTGCAGCATGTGGGGAGCTTTCATATCTGAAATTTGGTAAGAGCATACATTGCTATGCTGTTAAGGCCGATATTGATTCTGATATTTCTACTGGGACAGCCCTAGTCTCCATGTATGCTAAATGTGGATTCTTTACTCCTGCCTTGATTATATTCAACAGAATGCCATCTAAGGATGTTGTGACGTGGAATGCACTTATAAATGCATATACCCAAATAGGTGATGTCTACCATGCTATTGATATGTTGGATGAGTTATGGTTGTCTGGAATAAAACCTGATGCAGGAACGATGGTGGGTATGGTCTCGGCTTGTAGCTTATTAAATGATCTAAATCATGGTACTTGTATTCATGGACGAATTATAAAGAATGGGTTTGAACATGATGCACCTTTGAAAAATGCTCTTATTGGCATGTATTGCAAATGTGGTGACATTTCGTCAGCTGAATTTCTATTTAATAGAACAAAATTAATGAAAGATGTGGTATCCTGGAATGTAATGATTGCAGGATACATGCAGGGTGGATATGCCAGGGAAGCTATATCTGTGTTTCACCAGATGAAATTAGAGAACTGTCAGCCTAATTTAGTCACATTTGTTAGCATCCTTCCTGCAGTGGCATATTTAGCAGCATTACGAGAGGGCATGGCTTTTCATGCCTGCATTATCCAGAATGGGTTTCTATCTAATACACTTGTGGAAAATAGTCTTATTGACATGTATGCTAAATGCGGACAGCTCGATCATTCAGAAAAATGCTTTGAAGAGATGGAACTCAAGGACAAAGTTTCATGGAACGCAATGCTTGCAGCGTATGCAATGCACGGACAAGGGGATCATGCTATTGCACTTTTATCGCTGATGCAAAAGAGCTGTATCCAACTTGATTCTGTGTCATTCATCAGTCTCTTGTCTGCCTGCAGACATGCGGGTTTGATAAAAGAAGGAAAGGAAGTTTTTCAGGTCATGTGTGAAAAGTACCATCTTGAACCAGAGTTGGAACATTATGCTTGCATGGTAGATCTTCTAGGCCGTGCTGGTTTCTTTGATGAAGCTTTAGATTTGATTAATACGATGCCCATGGAACCTGATGCCGGAGTCTGGGGAGCATTTTTAGGGGCATGCAGGATGCATTCAAATGCAAAGTTGGGAGAAGTTGCGTTGAGTCATCTTGTTAACCTTGAGCCTACAAATGCAACCAATTACATAGCTTTATCAGATATACATGCACATTCTGCTAGATGGGGGGATGCTTGCAAGACAAGATCAAAGATGAAGAGCTCTGGGTCGAAGAAAATGCCAGGATGTAGTTGGGTAGAGGTCCAAAACAGGGTTCATACGCTACAGGCGGGTGACTACAACTACCCCCAACTTGGAAAAGAGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000018 GO:0000280 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0006139 GO:0006259 GO:0006310 GO:0006311 GO:0006325 GO:0006338 GO:0006342 GO:0006355 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007127 GO:0007131 GO:0008094 GO:0008150 GO:0008152 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0015616 GO:0016043 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0022402 GO:0022414 GO:0030491 GO:0030702 GO:0031047 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0033170 GO:0034641 GO:0035822 GO:0035825 GO:0035861 GO:0040029 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051052 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051321 GO:0060255 GO:0061806 GO:0061982 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090734 GO:0097159 GO:0140013 GO:0140097 GO:1901360 GO:1901363 GO:1902679 GO:1903046 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03440 map03440
Pfam Domains
Protein Families
Helicase_C Methyltransf_11 Methyltransf_21 SNF2_N

Protein Analysis

787

Amino Acids

86.89

Weight (kDa)

5.31

Isoelectric Point (pI)

36.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_1 PF12854 43 - 75 3.9e-06 PPR repeat
PPR_2 PF13041 78 - 115 2.2e-07 PPR repeat family
PPR_2 PF13041 382 - 429 2.4e-08 PPR repeat family
PPR_3 PF13812 481 - 530 2e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 484 - 528 3.8e-12 PPR repeat family
PPR PF01535 487 - 517 6.2e-07 PPR repeat
PPR_2 PF13041 586 - 632 8e-08 PPR repeat family
E_motif PF20431 703 - 765 4.8e-19 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1172, 1337
AccI GTMKAC 2 cut(s) 44, 1200
AccII CGCG 1 cut(s) 578
AciI CCGC 4 cut(s) 569, 1707, 1904, 2324
AclWI GGATC 4 cut(s) 278, 291, 709, 1818
AcsI RAATTY 3 cut(s) 340, 1004, 1421
AcuI CTGAAG 2 cut(s) 83, 918
AfaI GTAC 4 cut(s) 5, 575, 1315, 1955
AfiI CCNNNNNNNGG 5 cut(s) 1256, 2069, 2076, 2305, 2348
AflIII ACRYGT 1 cut(s) 1692
AgsI TTSAA 9 cut(s) 92, 453, 625, 1129, 1352, 1369, 1739, 1964, 2112
AhdI GACNNNNNGTC 1 cut(s) 1411
AjiI CACGTC 1 cut(s) 1158
AjnI CCWGG 3 cut(s) 1463, 1505, 2275
AjuI GAANNNNNNNTTGG 2 cut(s) 2292, 2324
Alw21I GWGCWC 3 cut(s) 111, 670, 2258
Alw26I GTCTC 5 cut(s) 823, 915, 1084, 1282, 1886
Alw44I GTGCAC 2 cut(s) 107, 666
AlwI GGATC 4 cut(s) 278, 291, 709, 1818
AoxI GGCC 2 cut(s) 1038, 2005
ApaLI GTGCAC 2 cut(s) 107, 666
ApeKI GCWGC 5 cut(s) 77, 677, 977, 1604, 1784
ApoI RAATTY 3 cut(s) 340, 1004, 1421
AseI ATTAAT 2 cut(s) 1445, 2043
Asp700I GAANNNNTTC 1 cut(s) 1932
AspS9I GGNCC 2 cut(s) 156, 2296
AsuC2I CCSGG 1 cut(s) 265
AsuHPI GGTGA 7 cut(s) 205, 349, 542, 1205, 1415, 1520, 2339
AvaII GGWCC 2 cut(s) 156, 2296
BaeGI GKGCMC 2 cut(s) 111, 670
BaeI ACNNNNGTAYC 2 cut(s) 565, 598
BamHI GGATCC 1 cut(s) 283
BanII GRGCYC 1 cut(s) 2258
Bbv12I GWGCWC 3 cut(s) 111, 670, 2258
BbvI GCAGC 5 cut(s) 64, 664, 989, 1616, 1796
BceAI ACGGC 1 cut(s) 1992
BcgI CGANNNNNNTGC 6 cut(s) 493, 527, 1812, 1846, 2254, 2288
BciT130I CCWGG 3 cut(s) 1465, 1507, 2277
BciVI GTATCC 3 cut(s) 1471, 1478, 1862
BcnI CCSGG 1 cut(s) 265
BcoDI GTCTC 5 cut(s) 823, 915, 1084, 1282, 1886
BfaI CTAG 5 cut(s) 29, 378, 1076, 2003, 2214
BfmI CTRYAG 3 cut(s) 1587, 1894, 2318
BfuI GTATCC 3 cut(s) 1471, 1478, 1862
BglI GCCNNNNNGGC 1 cut(s) 65
BglII AGATCT 1 cut(s) 1996
BisI GCNGC 5 cut(s) 78, 678, 978, 1605, 1785
BlsI GCNGC 5 cut(s) 79, 679, 979, 1606, 1786
Bme1390I CCNGG 4 cut(s) 265, 1465, 1507, 2277
Bme18I GGWCC 2 cut(s) 156, 2296
BmeRI GACNNNNNGTC 1 cut(s) 1411
BmgBI CACGTC 1 cut(s) 1158
BmgT120I GGNCC 2 cut(s) 156, 2296
BmiI GGNNCC 4 cut(s) 157, 285, 366, 2061
BmrFI CCNGG 4 cut(s) 265, 1465, 1507, 2277
BmrI ACTGGG 1 cut(s) 1074
BmsI GCATC 9 cut(s) 306, 1243, 1348, 1587, 1827, 2040, 2056, 2094, 2215
BmuI ACTGGG 1 cut(s) 1074
BpuEI CTTGAG 2 cut(s) 1736, 2177
BpuMI CCSGG 1 cut(s) 265
BsaBI GATNNNNATC 1 cut(s) 1305
BsaI GGTCTC 1 cut(s) 1282
BsaJI CCNNGG 2 cut(s) 1506, 2055
Bsc4I CCNNNNNNNGG 5 cut(s) 1256, 2069, 2076, 2305, 2348
Bse1I ACTGG 3 cut(s) 159, 883, 1069
Bse3DI GCAATG 5 cut(s) 326, 819, 1021, 1782, 1800
Bse8I GATNNNNATC 1 cut(s) 1305
BseBI CCWGG 3 cut(s) 1465, 1507, 2277
BseDI CCNNGG 2 cut(s) 1506, 2055
BseJI GATNNNNATC 1 cut(s) 1305
BseLI CCNNNNNNNGG 5 cut(s) 1256, 2069, 2076, 2305, 2348
BseMI GCAATG 5 cut(s) 326, 819, 1021, 1782, 1800
BseMII CTCAG 2 cut(s) 219, 675
BseNI ACTGG 3 cut(s) 159, 883, 1069
BseSI GKGCMC 2 cut(s) 111, 670
BseXI GCAGC 5 cut(s) 64, 664, 989, 1616, 1796
BsgI GTGCAG 3 cut(s) 222, 542, 846
Bsh1236I CGCG 1 cut(s) 578
BshFI GGCC 2 cut(s) 1040, 2007
BsiHKAI GWGCWC 3 cut(s) 111, 670, 2258
BsiSI CCGG 2 cut(s) 264, 2070
BslFI GGGAC 3 cut(s) 169, 571, 1081
BslI CCNNNNNNNGG 5 cut(s) 1256, 2069, 2076, 2305, 2348
BsmAI GTCTC 5 cut(s) 823, 915, 1084, 1282, 1886
BsmFI GGGAC 3 cut(s) 169, 571, 1081
BsmI GAATGC 4 cut(s) 228, 1140, 1168, 2107
BsnI GGCC 2 cut(s) 1040, 2007
Bso31I GGTCTC 1 cut(s) 1282
Bsp1286I GDGCHC 3 cut(s) 111, 670, 2258
Bsp143I GATC 7 cut(s) 283, 714, 1300, 1717, 1810, 1996, 2240
Bsp19I CCATGG 1 cut(s) 2055
BspACI CCGC 4 cut(s) 569, 1707, 1904, 2324
BspANI GGCC 2 cut(s) 1040, 2007
BspCNI CTCAG 2 cut(s) 220, 676
BspFNI CGCG 1 cut(s) 578
BspHI TCATGA 1 cut(s) 660
BspLI GGNNCC 4 cut(s) 157, 285, 366, 2061
BspMAI CTGCAG 2 cut(s) 1591, 1898
BspPI GGATC 4 cut(s) 278, 291, 709, 1818
BspQI GCTCTTC 1 cut(s) 2246
BspTNI GGTCTC 1 cut(s) 1282
BsrDI GCAATG 5 cut(s) 326, 819, 1021, 1782, 1800
BsrI ACTGG 3 cut(s) 159, 883, 1069
BssECI CCNNGG 2 cut(s) 1506, 2055
BssMI GATC 7 cut(s) 283, 714, 1300, 1717, 1810, 1996, 2240
BssNAI GTATAC 1 cut(s) 45
BssT1I CCWWGG 1 cut(s) 2055
Bst1107I GTATAC 1 cut(s) 45
Bst2UI CCWGG 3 cut(s) 1465, 1507, 2277
Bst4CI ACNGT 2 cut(s) 113, 1550
Bst6I CTCTTC 3 cut(s) 87, 1734, 2246
BstAPI GCANNNNNTGC 6 cut(s) 251, 1023, 1392, 1781, 1790, 2210
BstC8I GCNNGC 9 cut(s) 70, 502, 877, 1636, 1782, 1894, 1987, 2098, 2230
BstDEI CTNAG 3 cut(s) 228, 684, 1143
BstDSI CCRYGG 1 cut(s) 2055
BstENI CCTNNNNNAGG 1 cut(s) 1254
BstFNI CGCG 1 cut(s) 578
BstKTI GATC 7 cut(s) 286, 717, 1303, 1720, 1813, 1999, 2243
BstMAI GTCTC 5 cut(s) 823, 915, 1084, 1282, 1886
BstMBI GATC 7 cut(s) 283, 714, 1300, 1717, 1810, 1996, 2240
BstNI CCWGG 3 cut(s) 1465, 1507, 2277
BstNSI RCATGY 8 cut(s) 249, 984, 1390, 1492, 1696, 1904, 2100, 2204
BstSCI CCNGG 4 cut(s) 263, 1463, 1505, 2275
BstSFI CTRYAG 3 cut(s) 1587, 1894, 2318
BstSLI GKGCMC 2 cut(s) 111, 670
BstUI CGCG 1 cut(s) 578
BstV1I GCAGC 5 cut(s) 64, 664, 989, 1616, 1796
BstX2I RGATCY 3 cut(s) 283, 714, 1996
BstYI RGATCY 3 cut(s) 283, 714, 1996
BstZ17I GTATAC 1 cut(s) 45
BsuI GTATCC 3 cut(s) 1471, 1478, 1862
BsuRI GGCC 2 cut(s) 1040, 2007
BtgI CCRYGG 1 cut(s) 2055
BtrI CACGTC 1 cut(s) 1158
BtsI GCAGTG 4 cut(s) 72, 303, 378, 1596
BtsIMutI CAGTG 4 cut(s) 72, 303, 378, 1596
Cac8I GCNNGC 9 cut(s) 70, 502, 877, 1636, 1782, 1894, 1987, 2098, 2230
CciI TCATGA 1 cut(s) 660
Cfr13I GGNCC 2 cut(s) 156, 2296
CseI GACGC 2 cut(s) 868, 958
Csp6I GTAC 4 cut(s) 4, 574, 1314, 1954
CspCI CAANNNNNGTGG 2 cut(s) 931, 966
CviQI GTAC 4 cut(s) 4, 574, 1314, 1954
DdeI CTNAG 3 cut(s) 228, 684, 1143
DpnI GATC 7 cut(s) 285, 716, 1302, 1719, 1812, 1998, 2242
DpnII GATC 7 cut(s) 283, 714, 1300, 1717, 1810, 1996, 2240
DriI GACNNNNNGTC 1 cut(s) 1411
Eam1104I CTCTTC 3 cut(s) 87, 1734, 2246
Eam1105I GACNNNNNGTC 1 cut(s) 1411
EarI CTCTTC 3 cut(s) 87, 1734, 2246
Ecl136II GAGCTC 1 cut(s) 2256
Eco130I CCWWGG 1 cut(s) 2055
Eco24I GRGCYC 1 cut(s) 2258
Eco31I GGTCTC 1 cut(s) 1282
Eco47I GGWCC 2 cut(s) 156, 2296
Eco53kI GAGCTC 1 cut(s) 2256
Eco57I CTGAAG 2 cut(s) 83, 918
EcoICRI GAGCTC 1 cut(s) 2256
EcoNI CCTNNNNNAGG 1 cut(s) 1254
EcoRII CCWGG 3 cut(s) 1463, 1505, 2275
EcoT14I CCWWGG 1 cut(s) 2055
EcoT22I ATGCAT 2 cut(s) 1180, 2109
EcoT38I GRGCYC 1 cut(s) 2258
ErhI CCWWGG 1 cut(s) 2055
FalI AAGNNNNNCTT 2 cut(s) 32, 64
FaqI GGGAC 3 cut(s) 169, 571, 1081
FauI CCCGC 3 cut(s) 562, 1897, 2317
FblI GTMKAC 2 cut(s) 44, 1200
Fnu4HI GCNGC 5 cut(s) 78, 678, 978, 1605, 1785
FriOI GRGCYC 1 cut(s) 2258
Fsp4HI GCNGC 5 cut(s) 78, 678, 978, 1605, 1785
FspBI CTAG 5 cut(s) 29, 378, 1076, 2003, 2214
GluI GCNGC 5 cut(s) 78, 678, 978, 1605, 1785
HaeIII GGCC 2 cut(s) 1040, 2007
HapII CCGG 2 cut(s) 264, 2070
HgaI GACGC 2 cut(s) 868, 958
HincII GTYRAC 1 cut(s) 2152
HindII GTYRAC 1 cut(s) 2152
HindIII AAGCTT 1 cut(s) 2028
HinfI GANTC 7 cut(s) 395, 746, 1049, 1101, 1862, 2073, 2140
HpaI GTTAAC 1 cut(s) 2152
HpaII CCGG 2 cut(s) 264, 2070
HphI GGTGA 7 cut(s) 205, 349, 542, 1205, 1415, 1520, 2339
Hpy166II GTNNAC 5 cut(s) 45, 109, 668, 1201, 2152
Hpy188I TCNGA 6 cut(s) 165, 751, 1002, 1054, 1726, 2194
Hpy188III TCNNGA 9 cut(s) 29, 378, 514, 661, 896, 920, 1239, 1645, 1961
Hpy8I GTNNAC 5 cut(s) 45, 109, 668, 1201, 2152
HpyAV CCTTC 4 cut(s) 58, 802, 1592, 1913
HpyCH4III ACNGT 2 cut(s) 113, 1550
HpyCH4IV ACGT 1 cut(s) 1157
HpyF3I CTNAG 3 cut(s) 228, 684, 1143
HpySE526I ACGT 1 cut(s) 1157
KspAI GTTAAC 1 cut(s) 2152
Kzo9I GATC 7 cut(s) 283, 714, 1300, 1717, 1810, 1996, 2240
LguI GCTCTTC 1 cut(s) 2246
LmnI GCTCC 2 cut(s) 988, 2081
Lsp1109I GCAGC 5 cut(s) 64, 664, 989, 1616, 1796
LweI GCATC 9 cut(s) 306, 1243, 1348, 1587, 1827, 2040, 2056, 2094, 2215
MaeI CTAG 5 cut(s) 29, 378, 1076, 2003, 2214
MaeII ACGT 1 cut(s) 1157
MaeIII GTNAC 4 cut(s) 1153, 1403, 1564, 2327
MalI GATC 7 cut(s) 285, 716, 1302, 1719, 1812, 1998, 2242
MboI GATC 7 cut(s) 283, 714, 1300, 1717, 1810, 1996, 2240
MboII GAAGA 5 cut(s) 104, 1751, 1991, 2263, 2278
MfeI CAATTG 1 cut(s) 818
MflI RGATCY 3 cut(s) 283, 714, 1996
MhlI GDGCHC 3 cut(s) 111, 670, 2258
MluCI AATT 9 cut(s) 340, 818, 1004, 1332, 1421, 1442, 1538, 1558, 2176
MlyI GAGTC 2 cut(s) 2082, 2149
MmeI TCCRAC 3 cut(s) 1200, 1879, 1953
Mph1103I ATGCAT 2 cut(s) 1180, 2109
MroXI GAANNNNTTC 1 cut(s) 1932
MseI TTAA 9 cut(s) 17, 51, 357, 1035, 1295, 1431, 1445, 2043, 2151
MslI CAYNNNNRTG 4 cut(s) 417, 723, 1026, 2112
MspA1I CMGCKG 1 cut(s) 1418
MspI CCGG 2 cut(s) 264, 2070
MspR9I CCNGG 4 cut(s) 265, 1465, 1507, 2277
MunI CAATTG 1 cut(s) 818
Mva1269I GAATGC 4 cut(s) 228, 1140, 1168, 2107
MvaI CCWGG 3 cut(s) 1465, 1507, 2277
MvnI CGCG 1 cut(s) 578
NciI CCSGG 1 cut(s) 265
NcoI CCATGG 1 cut(s) 2055
NdeII GATC 7 cut(s) 283, 714, 1300, 1717, 1810, 1996, 2240
NlaIV GGNNCC 4 cut(s) 157, 285, 366, 2061
NmeAIII GCCGAG 1 cut(s) 1259
NmuCI GTSAC 4 cut(s) 1153, 1403, 1564, 2327
NsiI ATGCAT 2 cut(s) 1180, 2109
NspI RCATGY 8 cut(s) 249, 984, 1390, 1492, 1696, 1904, 2100, 2204
PaeI GCATGC 1 cut(s) 2100
PagI TCATGA 1 cut(s) 660
PciI ACATGT 1 cut(s) 1692
PciSI GCTCTTC 1 cut(s) 2246
PctI GAATGC 4 cut(s) 228, 1140, 1168, 2107
PdmI GAANNNNTTC 1 cut(s) 1932
PfeI GAWTC 5 cut(s) 395, 746, 1049, 1101, 1862
PfoI TCCNGGA 1 cut(s) 1463
PkrI GCNGC 5 cut(s) 79, 679, 979, 1606, 1786
PleI GAGTC 2 cut(s) 2081, 2148
PpsI GAGTC 2 cut(s) 2081, 2148
PscI ACATGT 1 cut(s) 1692
PshBI ATTAAT 2 cut(s) 1445, 2043
PsiI TTATAA 2 cut(s) 1172, 1337
Psp124BI GAGCTC 1 cut(s) 2258
Psp6I CCWGG 3 cut(s) 1463, 1505, 2275
PspGI CCWGG 3 cut(s) 1463, 1505, 2275
PspN4I GGNNCC 4 cut(s) 157, 285, 366, 2061
PspPI GGNCC 2 cut(s) 156, 2296
PstI CTGCAG 2 cut(s) 1591, 1898
PsuI RGATCY 3 cut(s) 283, 714, 1996
PvuII CAGCTG 1 cut(s) 1418
RsaI GTAC 4 cut(s) 5, 575, 1315, 1955
RsaNI GTAC 4 cut(s) 4, 574, 1314, 1954
RseI CAYNNNNRTG 4 cut(s) 417, 723, 1026, 2112
SacI GAGCTC 1 cut(s) 2258
SapI GCTCTTC 1 cut(s) 2246
SaqAI TTAA 9 cut(s) 17, 51, 357, 1035, 1295, 1431, 1445, 2043, 2151
SatI GCNGC 5 cut(s) 78, 678, 978, 1605, 1785
Sau3AI GATC 7 cut(s) 283, 714, 1300, 1717, 1810, 1996, 2240
Sau96I GGNCC 2 cut(s) 156, 2296
SchI GAGTC 2 cut(s) 2082, 2149
ScrFI CCNGG 4 cut(s) 265, 1465, 1507, 2277
SduI GDGCHC 3 cut(s) 111, 670, 2258
SfaNI GCATC 9 cut(s) 306, 1243, 1348, 1587, 1827, 2040, 2056, 2094, 2215
SfcI CTRYAG 3 cut(s) 1587, 1894, 2318
SinI GGWCC 2 cut(s) 156, 2296
SmiMI CAYNNNNRTG 4 cut(s) 417, 723, 1026, 2112
SmlI CTYRAG 2 cut(s) 1751, 2156
SmoI CTYRAG 2 cut(s) 1751, 2156
SphI GCATGC 1 cut(s) 2100
Sse9I AATT 9 cut(s) 340, 818, 1004, 1332, 1421, 1442, 1538, 1558, 2176
SsiI CCGC 4 cut(s) 569, 1707, 1904, 2324
SspMI CTAG 5 cut(s) 29, 378, 1076, 2003, 2214
SstI GAGCTC 1 cut(s) 2258
StyD4I CCNGG 4 cut(s) 263, 1463, 1505, 2275
StyI CCWWGG 1 cut(s) 2055
TaaI ACNGT 2 cut(s) 113, 1550
TaiI ACGT 1 cut(s) 1160
TaqI TCGA 5 cut(s) 513, 744, 919, 1716, 2264
TasI AATT 9 cut(s) 340, 818, 1004, 1332, 1421, 1442, 1538, 1558, 2176
TfiI GAWTC 5 cut(s) 395, 746, 1049, 1101, 1862
Tru1I TTAA 9 cut(s) 17, 51, 357, 1035, 1295, 1431, 1445, 2043, 2151
Tru9I TTAA 9 cut(s) 17, 51, 357, 1035, 1295, 1431, 1445, 2043, 2151
TscAI CASTG 4 cut(s) 79, 303, 378, 1596
TseFI GTSAC 4 cut(s) 1153, 1403, 1564, 2327
TseI GCWGC 5 cut(s) 77, 677, 977, 1604, 1784
Tsp45I GTSAC 4 cut(s) 1153, 1403, 1564, 2327
TspGWI ACGGA 1 cut(s) 1815
TspRI CASTG 4 cut(s) 79, 303, 378, 1596
VneI GTGCAC 2 cut(s) 107, 666
VpaK11BI GGWCC 2 cut(s) 156, 2296
VspI ATTAAT 2 cut(s) 1445, 2043
XagI CCTNNNNNAGG 1 cut(s) 1254
XapI RAATTY 3 cut(s) 340, 1004, 1421
XbaI TCTAGA 2 cut(s) 28, 377
XceI RCATGY 8 cut(s) 249, 984, 1390, 1492, 1696, 1904, 2100, 2204
XcmI CCANNNNNNNNNTGG 1 cut(s) 2283
XmiI GTMKAC 2 cut(s) 44, 1200
XmnI GAANNNNTTC 1 cut(s) 1932
XspI CTAG 5 cut(s) 29, 378, 1076, 2003, 2214
Zsp2I ATGCAT 2 cut(s) 1180, 2109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.