Rmu_sc0003714.1_g000006

DNA repair and recombination protein RAD54-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003714.1
OG0005309
Physical Location & Seq
Forward (+)
26179 .. 33873
7695 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003714.1_g000006.1.cds

Sequence Viewer

Length: 4071 bp
atgtctactgacaaaattttgttgaaattaggtgcccggaagatggtgaaccggtggaatatttgctgcgggttggatggagcttccagtggccaattcggtagatttctgcccggttcttggggtggcgccaccggttctgaacttctgggatcgagttcttcaaggtgtaaggtggaggcagaaaatgcttcaagatacgacagacttgacatcagaatgaagataaagaagaggcatctgtatcgagctaagcatccatttgatgctcatctgtttgaggctctttgctgtggatcatggaaaggtgtggaacttctaagaattagcaatggaactatgaacatgaaattcatagaccatccatgtgtgattctggataaaggtccatttgccaaccttcgaataaaatcaagaagagcaactttatatgattgcatttgcttgttgaggcctggtgtggatatctgtgtgctttcaaactctgatcatacagagagttcagataaggaaaggcgagatccggtgtgggttgatgctagaataagttctatcgaaagggagcctcatgactctcaatgctcatgccagtttcatgtcaacttttatgttaaccaaggacctcttggttcagagagagcaacacttaaaaaagaaactgaagttattggaattgatcaggttttcatcttgcaaaggcttgataggaattcttgtaaaaatcagtactaccgttgggatcgctctgtggactgctctactctgccaaacactaaattattattggggaaattcttatcggacttgtcatggttgcttgttacatcttcattgaagcagattgtatttgatgtaagatcagtgcagaacaagattgtgtatcaaatttctggtggttatgatgacacaactccgtcaaactctgaaaactcttttaaagctgtgaatttcggagtagagaatgggatcccggtacctataatagttcagttggttccagatgatgctattgggggtgatcctacttgtgacatgcatgaagatagaccatctccaatttctgaggttccagaattgcggcgttccaagcgtcgaaagatacaacctgatcgtttccttgcatgtgatgctccctctgagatacaaataggccccattcgaagcaggccatataagatagatcaatcccaagatgattctgatgacgaattgtacctaccactgtcatacctgtttcgtaagaaggcaagcaactctttggaagaggatagtactgaggcagagcaaaatgctggttctaagaggaagcgatcaaatcccagtgacaatgatgatctattcgaatgcaaagaaagcaagattaagtggatgaaagtgaaatcaggtgtggctaaaaataagacgaagaaacatcgagctgatcaacttgccattgttcctgcatctgtcaaaagtgatcagctaacccttggaaacttccctccaaatgccaatggtcctgcaaatcgttcaaaagacagtggtgaatttcctaccaagcattattatcgtttcagtacttctaaagcacagaaacagaaaagaaacaaaattgctggcttggaagacatggatgttcaaactaaatgggatgggggtgcatcctctagtagatttccgagaagaggctatcattacggatatcatcatccctctatacgatcaaaaaggacggatcggtatgctggagcttattcaaagaggtccttaaatgcaggtgcatacaaggagctgataaataagttcttgaaagacatggattgctcaaacaagcaagaaccaaacattatggaccagtggaaagatttcaaggcaaacaagaactctgaccaaaaggacgaaactgaaatgccagaagatgaggaggaggaggaaatgtctgaggaggatatgttgtggaaagaatttgaattagtaatggcatcatgttatcttcttggtgatgaggaatcaaatggtgctgcttcaggtaaatttgcacaaaagtcaggtccaggttgcaagcatgacttcactctcgatgaagaaattgggcttaagtgcattatatgtggctttgtgaagactgaaatcaaacatgttacacccccctttgtgcaaaacccgtatgggttcacagatgataaaaaaccaaatgaagaagatccaccaccagatcctaaaagagctgaatatgaggagtttgatttcttccacaaacgtgataaccctgtggatgagcatatttcagaagaaaatgacaatgtttgggctttaattcctgaacttagaaagaaactgttattccaccagaagaaggcttttgagtttttatggaagaatattgcaggatctatggaaccagcattgatggaaaagaaatccaagagaaatggtggctgtgttatatctcatactcctggagctgggaaaacttttttgatcattgcatttcttgttagctacctgaagttattccctggaaaacgacctttagtccttgctccaaagacaacactctacacctggtacaaggaattcattaaatggaagatccctataccggtttatctaattcacggtcgtcgaacctatagagtgtttaggaataagtcagtaacctatacaagaggcccgaagccaactgacgatgtgatgcatgttctcgattgcctagaaaagatacagaagtggcatgcacaacctagtgttcttgtcatggggtatacttctttcctaacattgatgcgtgaagactcaaagtttgtccacagaagatttatggctcaagtcctgagggaaagtccagggctcctagttctagatgaagggcacaaccccagaagtaccaaatcaaggttgaggaaaggattgatgaaagttgaaactgatttgagaatattgctttcaggtactttgtttcagaacaatttctgtgaatacttcaacacactttgtttggcaaggccgaagtttgtgaatgaagttttgaagtcattggacccaaaatacagaaggaaaaagaaggcattgaaagacaaagcacgccatttgatggaagctcgagccagaaagttgttcttggataaaattgccaagaaaattgattcaaatgaagaagaagatcagaggatcgaaggtctcaacaagctgagaaagataacaaacagatttatagatgtgtatgaaggtggaaattcagacacccttcccggtctacagatttacaccttgctgatgaacaccactgacattcaacaagtgattttggatagactgcaacaaataatgtccaagtacaaagggtatcccttggaactggaacttctgatcacccttggatcaatacatccttggctaatcaaaacggcagcttgtgctgataagttctttagtccccaagaactgatggagcttgagcaatacaagtatgatttgcacaaagggtcaaaagtcaagtttgttttgaacctggtttaccgtgttactcgaaaagagaaggtcttgattttctgccacaacattgcacctgtgagactgtttctagaattgtttgagagggtctttcagtgggagcgaggcagagaagttttggttcttaccggggatctggagttatttgaacgtggaaaggttatggacaaatttgaagaaccaggtggagcctcaaaggttctgcttgcatctatcactgcttgtgctgaaggcattagtctgacagctgcatctagggttatcttgttggactccgaatggaatccttccaagacgaagcaagctatagcacgagcttttaggcctggtcagcaaaaggttgtgtatgtttatcagctattggcaacaggaacgctcgaagaggacaagtatagaaggactacatggaaagagtgggtatccagcatgatattcagtgaggcatttgtggaggatccttctcgctggcaagctgagaagattgaagatgatatattgagggaaatggtgggtgaagacaagagtaaagcattccatatgatcatgaaaaatgaaaaggcttcaacacatacttcatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000018 GO:0000280 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0006139 GO:0006259 GO:0006310 GO:0006311 GO:0006325 GO:0006338 GO:0006342 GO:0006355 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007127 GO:0007131 GO:0008094 GO:0008150 GO:0008152 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0015616 GO:0016043 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0022402 GO:0022414 GO:0030491 GO:0030702 GO:0031047 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0033170 GO:0034641 GO:0035822 GO:0035825 GO:0035861 GO:0040029 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051052 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051321 GO:0060255 GO:0061806 GO:0061982 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090734 GO:0097159 GO:0140013 GO:0140097 GO:1901360 GO:1901363 GO:1902679 GO:1903046 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03440 map03440
Pfam Domains
Protein Families
Helicase_C Methyltransf_11 Methyltransf_21 SNF2_N

Protein Analysis

1356

Amino Acids

155.6

Weight (kDa)

8.93

Isoelectric Point (pI)

48.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1745
Acc36I ACCTGC 1 cut(s) 1745
Acc65I GGTACC 1 cut(s) 973
AccB1I GGYRCC 3 cut(s) 32, 128, 973
AccB7I CCANNNNNTGG 1 cut(s) 3073
AccI GTMKAC 3 cut(s) 5, 2744, 3235
AciI CCGC 2 cut(s) 69, 1078
AcoI YGGCCR 1 cut(s) 91
AcuI CTGAAG 4 cut(s) 681, 1989, 2507, 3741
AcyI GRCGYC 1 cut(s) 129
AfaI GTAC 9 cut(s) 728, 975, 1214, 1273, 1558, 2549, 2866, 2932, 3317
AfiI CCNNNNNNNGG 8 cut(s) 43, 120, 1664, 2335, 2444, 2581, 2874, 3073
AflII CTTAAG 1 cut(s) 2075
AflIII ACRYGT 1 cut(s) 2116
AgeI ACCGGT 3 cut(s) 51, 134, 2581
AhdI GACNNNNNGTC 1 cut(s) 2513
AjnI CCWGG 9 cut(s) 454, 2032, 2437, 2497, 2543, 2824, 3489, 3673, 3817
AleI CACNNNNGTG 1 cut(s) 2238
Alw26I GTCTC 2 cut(s) 3164, 3547
Ama87I CYCGRG 1 cut(s) 3081
AoxI GGCC 7 cut(s) 91, 452, 1150, 1166, 2650, 2984, 3815
ApeKI GCWGC 4 cut(s) 66, 2000, 3389, 3740
ArsI GACNNNNNNTTYG 4 cut(s) 2014, 2046, 2765, 2797
AsiGI ACCGGT 3 cut(s) 51, 134, 2581
Asp700I GAANNNNTTC 4 cut(s) 547, 1844, 2492, 2948
Asp718I GGTACC 1 cut(s) 973
AspLEI GCGC 1 cut(s) 131
AspS9I GGNCC 9 cut(s) 386, 620, 1151, 1496, 1743, 1831, 2030, 2651, 3019
AsuC2I CCSGG 5 cut(s) 37, 114, 971, 3231, 3622
AsuHPI GGTGA 6 cut(s) 58, 1028, 1535, 1991, 3343, 4016
AsuII TTCGAA 3 cut(s) 403, 1159, 1341
AvaI CYCGRG 1 cut(s) 3081
AvaII GGWCC 7 cut(s) 386, 620, 1496, 1743, 1831, 2030, 3019
AxyI CCTNAGG 1 cut(s) 2813
BaeGI GKGCMC 2 cut(s) 37, 2853
BaeI ACNNNNGTAYC 2 cut(s) 2693, 2726
BalI TGGCCA 1 cut(s) 93
BamHI GGATCC 2 cut(s) 966, 3946
BanI GGYRCC 3 cut(s) 32, 128, 973
BanII GRGCYC 1 cut(s) 2832
BauI CACGAG 1 cut(s) 3804
BbsI GAAGAC 4 cut(s) 1611, 2108, 2778, 4014
BbvI GCAGC 4 cut(s) 53, 1987, 3401, 3727
BccI CCATC 8 cut(s) 37, 71, 369, 1057, 1625, 2383, 3067, 3421
BceAI ACGGC 1 cut(s) 3402
BcgI CGANNNNNNTGC 4 cut(s) 227, 261, 1529, 1563
BciT130I CCWGG 9 cut(s) 456, 2034, 2439, 2499, 2545, 2826, 3491, 3675, 3819
BciVI GTATCC 2 cut(s) 3336, 3922
BclI TGATCA 7 cut(s) 487, 676, 1420, 1456, 2460, 3348, 4032
BcnI CCSGG 5 cut(s) 37, 114, 971, 3231, 3622
BcoDI GTCTC 2 cut(s) 3164, 3547
BfaI CTAG 8 cut(s) 540, 1647, 2693, 2724, 2834, 2840, 3563, 3747
BfmI CTRYAG 3 cut(s) 2611, 3236, 3798
BfoI RGCGCY 1 cut(s) 132
BfrI CTTAAG 1 cut(s) 2075
BfuAI ACCTGC 1 cut(s) 1745
BfuI GTATCC 2 cut(s) 3336, 3922
BisI GCNGC 5 cut(s) 67, 1079, 2001, 3390, 3741
BlpI GCTNAGC 1 cut(s) 252
BlsI GCNGC 5 cut(s) 68, 1080, 2002, 3391, 3742
BmcAI AGTACT 3 cut(s) 728, 1273, 1558
Bme18I GGWCC 7 cut(s) 386, 620, 1496, 1743, 1831, 2030, 3019
BmeRI GACNNNNNGTC 1 cut(s) 2513
BmeT110I CYCGRG 1 cut(s) 3081
BmgT120I GGNCC 9 cut(s) 386, 620, 1151, 1496, 1743, 1831, 2030, 2651, 3019
BmrI ACTGGG 1 cut(s) 1314
BmuI ACTGGG 1 cut(s) 1314
BpiI GAAGAC 4 cut(s) 1611, 2108, 2778, 4014
BpmI CTGGAG 3 cut(s) 1746, 2460, 3650
Bpu1102I GCTNAGC 1 cut(s) 252
Bpu14I TTCGAA 3 cut(s) 403, 1159, 1341
BpuEI CTTGAG 2 cut(s) 2790, 3455
BpuMI CCSGG 5 cut(s) 37, 114, 971, 3231, 3622
BsaBI GATNNNNATC 1 cut(s) 270
BsaHI GRCGYC 1 cut(s) 129
BsaI GGTCTC 1 cut(s) 3164
BsaJI CCNNGG 8 cut(s) 616, 1468, 2497, 2825, 3330, 3355, 3371, 3621
BsaWI WCCGGW 4 cut(s) 51, 134, 523, 2581
BsaXI ACNNNNNCTCC 4 cut(s) 1901, 1916, 1931, 1946
Bsc4I CCNNNNNNNGG 8 cut(s) 43, 120, 1664, 2335, 2444, 2581, 2874, 3073
Bse118I RCCGGY 3 cut(s) 51, 134, 2581
Bse1I ACTGG 5 cut(s) 87, 589, 1320, 1834, 3342
Bse21I CCTNAGG 1 cut(s) 2813
Bse3DI GCAATG 3 cut(s) 337, 2463, 3540
Bse8I GATNNNNATC 1 cut(s) 270
BseBI CCWGG 9 cut(s) 456, 2034, 2439, 2499, 2545, 2826, 3491, 3675, 3819
BseDI CCNNGG 8 cut(s) 616, 1468, 2497, 2825, 3330, 3355, 3371, 3621
BseJI GATNNNNATC 1 cut(s) 270
BseLI CCNNNNNNNGG 8 cut(s) 43, 120, 1664, 2335, 2444, 2581, 2874, 3073
BseMI GCAATG 3 cut(s) 337, 2463, 3540
BseMII CTCAG 7 cut(s) 1053, 1128, 1266, 1911, 2804, 3161, 3957
BseNI ACTGG 5 cut(s) 87, 589, 1320, 1834, 3342
BseRI GAGGAG 5 cut(s) 1916, 1919, 1922, 1937, 2231
BseSI GKGCMC 2 cut(s) 37, 2853
BseXI GCAGC 4 cut(s) 53, 1987, 3401, 3727
BseYI CCCAGC 1 cut(s) 2444
BsgI GTGCAG 1 cut(s) 884
Bsh1285I CGRYCG 1 cut(s) 2602
BshFI GGCC 7 cut(s) 93, 454, 1152, 1168, 2652, 2986, 3817
BshNI GGYRCC 3 cut(s) 32, 128, 973
BshTI ACCGGT 3 cut(s) 51, 134, 2581
BsiEI CGRYCG 1 cut(s) 2602
BsiHKCI CYCGRG 1 cut(s) 3081
BsiSI CCGG 9 cut(s) 37, 52, 114, 135, 524, 971, 2582, 3231, 3621
BslFI GGGAC 1 cut(s) 3399
BslI CCNNNNNNNGG 8 cut(s) 43, 120, 1664, 2335, 2444, 2581, 2874, 3073
BsmAI GTCTC 2 cut(s) 3164, 3547
BsmFI GGGAC 1 cut(s) 3399
BsmI GAATGC 2 cut(s) 1349, 4022
BsnI GGCC 7 cut(s) 93, 454, 1152, 1168, 2652, 2986, 3817
Bso31I GGTCTC 1 cut(s) 3164
BsoBI CYCGRG 1 cut(s) 3081
Bsp119I TTCGAA 3 cut(s) 403, 1159, 1341
Bsp1286I GDGCHC 3 cut(s) 37, 2832, 2853
Bsp1720I GCTNAGC 1 cut(s) 252
BspACI CCGC 2 cut(s) 69, 1078
BspANI GGCC 7 cut(s) 93, 454, 1152, 1168, 2652, 2986, 3817
BspCNI CTCAG 7 cut(s) 1054, 1129, 1267, 1912, 2805, 3162, 3958
BspHI TCATGA 2 cut(s) 568, 4035
BspMI ACCTGC 1 cut(s) 1745
BspQI GCTCTTC 1 cut(s) 412
BspT104I TTCGAA 3 cut(s) 403, 1159, 1341
BspT107I GGYRCC 3 cut(s) 32, 128, 973
BspTI CTTAAG 1 cut(s) 2075
BspTNI GGTCTC 1 cut(s) 3164
BsrDI GCAATG 3 cut(s) 337, 2463, 3540
BsrFI RCCGGY 3 cut(s) 51, 134, 2581
BsrI ACTGG 5 cut(s) 87, 589, 1320, 1834, 3342
BssAI RCCGGY 3 cut(s) 51, 134, 2581
BssECI CCNNGG 8 cut(s) 616, 1468, 2497, 2825, 3330, 3355, 3371, 3621
BssNAI GTATAC 1 cut(s) 2745
BssNI GRCGYC 1 cut(s) 129
BssSI CACGAG 1 cut(s) 3804
BssT1I CCWWGG 5 cut(s) 616, 1468, 3330, 3355, 3371
Bst1107I GTATAC 1 cut(s) 2745
Bst2BI CACGAG 1 cut(s) 3804
Bst2UI CCWGG 9 cut(s) 456, 2034, 2439, 2499, 2545, 2826, 3491, 3675, 3819
Bst4CI ACNGT 7 cut(s) 734, 1224, 1520, 2319, 2600, 3500, 3558
Bst6I CTCTTC 5 cut(s) 227, 412, 1257, 1657, 3867
BstACI GRCGYC 1 cut(s) 129
BstAFI CTTAAG 1 cut(s) 2075
BstAPI GCANNNNNTGC 3 cut(s) 188, 1127, 3395
BstBI TTCGAA 3 cut(s) 403, 1159, 1341
BstH2I RGCGCY 1 cut(s) 132
BstHHI GCGC 1 cut(s) 131
BstMAI GTCTC 2 cut(s) 3164, 3547
BstMCI CGRYCG 1 cut(s) 2602
BstMWI GCNNNNNNNGC 6 cut(s) 188, 1087, 1127, 1353, 3395, 3823
BstNI CCWGG 9 cut(s) 456, 2034, 2439, 2499, 2545, 2826, 3491, 3675, 3819
BstNSI RCATGY 5 cut(s) 1036, 1125, 2120, 2681, 2717
BstSFI CTRYAG 3 cut(s) 2611, 3236, 3798
BstSLI GKGCMC 2 cut(s) 37, 2853
BstV1I GCAGC 4 cut(s) 53, 1987, 3401, 3727
BstV2I GAAGAC 4 cut(s) 1611, 2108, 2778, 4014
BstX2I RGATCY 8 cut(s) 520, 966, 2182, 2194, 2369, 2571, 3625, 3946
BstYI RGATCY 8 cut(s) 520, 966, 2182, 2194, 2369, 2571, 3625, 3946
BstZ17I GTATAC 1 cut(s) 2745
Bsu36I CCTNAGG 1 cut(s) 2813
BsuI GTATCC 2 cut(s) 3336, 3922
BsuRI GGCC 7 cut(s) 93, 454, 1152, 1168, 2652, 2986, 3817
BtsI GCAGTG 1 cut(s) 3708
BveI ACCTGC 1 cut(s) 1745
CciI TCATGA 2 cut(s) 568, 4035
CfoI GCGC 1 cut(s) 131
Cfr10I RCCGGY 3 cut(s) 51, 134, 2581
Cfr13I GGNCC 9 cut(s) 386, 620, 1151, 1496, 1743, 1831, 2030, 2651, 3019
CseI GACGC 1 cut(s) 1079
CsiI ACCWGGT 3 cut(s) 2543, 3489, 3673
Csp6I GTAC 9 cut(s) 727, 974, 1213, 1272, 1557, 2548, 2865, 2931, 3316
CspAI ACCGGT 3 cut(s) 51, 134, 2581
CviQI GTAC 9 cut(s) 727, 974, 1213, 1272, 1557, 2548, 2865, 2931, 3316
DinI GGCGCC 1 cut(s) 130
DraI TTTAAA 1 cut(s) 937
DriI GACNNNNNGTC 1 cut(s) 2513
EaeI YGGCCR 1 cut(s) 91
Eam1104I CTCTTC 5 cut(s) 227, 412, 1257, 1657, 3867
Eam1105I GACNNNNNGTC 1 cut(s) 2513
EarI CTCTTC 5 cut(s) 227, 412, 1257, 1657, 3867
Eco130I CCWWGG 5 cut(s) 616, 1468, 3330, 3355, 3371
Eco147I AGGCCT 2 cut(s) 454, 3817
Eco24I GRGCYC 1 cut(s) 2832
Eco31I GGTCTC 1 cut(s) 3164
Eco32I GATATC 2 cut(s) 466, 1682
Eco47I GGWCC 7 cut(s) 386, 620, 1496, 1743, 1831, 2030, 3019
Eco57I CTGAAG 4 cut(s) 681, 1989, 2507, 3741
Eco81I CCTNAGG 1 cut(s) 2813
Eco88I CYCGRG 1 cut(s) 3081
EcoO109I RGGNCCY 3 cut(s) 620, 1151, 1743
EcoRI GAATTC 2 cut(s) 709, 2555
EcoRII CCWGG 9 cut(s) 454, 2032, 2437, 2497, 2543, 2824, 3489, 3673, 3817
EcoRV GATATC 2 cut(s) 466, 1682
EcoT14I CCWWGG 5 cut(s) 616, 1468, 3330, 3355, 3371
EcoT22I ATGCAT 2 cut(s) 1038, 2679
EcoT38I GRGCYC 1 cut(s) 2832
EgeI GGCGCC 1 cut(s) 130
EheI GGCGCC 1 cut(s) 130
ErhI CCWWGG 5 cut(s) 616, 1468, 3330, 3355, 3371
FaqI GGGAC 1 cut(s) 3399
FauI CCCGC 1 cut(s) 62
FauNDI CATATG 1 cut(s) 4029
FbaI TGATCA 7 cut(s) 487, 676, 1420, 1456, 2460, 3348, 4032
FblI GTMKAC 3 cut(s) 5, 2744, 3235
Fnu4HI GCNGC 5 cut(s) 67, 1079, 2001, 3390, 3741
FriOI GRGCYC 1 cut(s) 2832
Fsp4HI GCNGC 5 cut(s) 67, 1079, 2001, 3390, 3741
FspBI CTAG 8 cut(s) 540, 1647, 2693, 2724, 2834, 2840, 3563, 3747
GlaI GCGC 1 cut(s) 130
GluI GCNGC 5 cut(s) 67, 1079, 2001, 3390, 3741
GsaI CCCAGC 1 cut(s) 2448
GsuI CTGGAG 3 cut(s) 1746, 2460, 3650
HaeII RGCGCY 1 cut(s) 132
HaeIII GGCC 7 cut(s) 93, 454, 1152, 1168, 2652, 2986, 3817
HapII CCGG 9 cut(s) 37, 52, 114, 135, 524, 971, 2582, 3231, 3621
HgaI GACGC 1 cut(s) 1079
HhaI GCGC 1 cut(s) 131
Hin1I GRCGYC 1 cut(s) 129
Hin6I GCGC 1 cut(s) 129
HinP1I GCGC 1 cut(s) 129
HincII GTYRAC 2 cut(s) 601, 613
HindII GTYRAC 2 cut(s) 601, 613
HinfI GANTC 8 cut(s) 373, 572, 1196, 1988, 2774, 3125, 3764, 3775
HpaI GTTAAC 1 cut(s) 613
HpaII CCGG 9 cut(s) 37, 52, 114, 135, 524, 971, 2582, 3231, 3621
HphI GGTGA 6 cut(s) 58, 1028, 1535, 1991, 3343, 4016
Hpy99I CGWCG 2 cut(s) 1095, 2607
HpyCH4III ACNGT 7 cut(s) 734, 1224, 1520, 2319, 2600, 3500, 3558
HpyCH4IV ACGT 2 cut(s) 2239, 3643
HpyF10VI GCNNNNNNNGC 6 cut(s) 188, 1087, 1127, 1353, 3395, 3823
HpySE526I ACGT 2 cut(s) 2239, 3643
Hsp92I GRCGYC 1 cut(s) 129
HspAI GCGC 1 cut(s) 129
KasI GGCGCC 1 cut(s) 128
KpnI GGTACC 1 cut(s) 977
Ksp22I TGATCA 7 cut(s) 487, 676, 1420, 1456, 2460, 3348, 4032
KspAI GTTAAC 1 cut(s) 613
LguI GCTCTTC 1 cut(s) 412
Lsp1109I GCAGC 4 cut(s) 53, 1987, 3401, 3727
MabI ACCWGGT 3 cut(s) 2543, 3489, 3673
MaeI CTAG 8 cut(s) 540, 1647, 2693, 2724, 2834, 2840, 3563, 3747
MaeII ACGT 2 cut(s) 2239, 3643
MaeIII GTNAC 6 cut(s) 820, 1028, 1322, 2119, 2635, 3502
MflI RGATCY 8 cut(s) 520, 966, 2182, 2194, 2369, 2571, 3625, 3946
MhlI GDGCHC 3 cut(s) 37, 2832, 2853
MlsI TGGCCA 1 cut(s) 93
MluNI TGGCCA 1 cut(s) 93
Mly113I GGCGCC 1 cut(s) 129
MlyI GAGTC 3 cut(s) 566, 2768, 3758
MmeI TCCRAC 2 cut(s) 54, 3741
Mox20I TGGCCA 1 cut(s) 93
Mph1103I ATGCAT 2 cut(s) 1038, 2679
MroXI GAANNNNTTC 4 cut(s) 547, 1844, 2492, 2948
MscI TGGCCA 1 cut(s) 93
MseI TTAA 8 cut(s) 612, 648, 936, 1362, 1748, 2076, 2294, 2562
MslI CAYNNNNRTG 3 cut(s) 218, 366, 2238
Msp20I TGGCCA 1 cut(s) 93
MspA1I CMGCKG 1 cut(s) 3740
MspCI CTTAAG 1 cut(s) 2075
MspI CCGG 9 cut(s) 37, 52, 114, 135, 524, 971, 2582, 3231, 3621
Mva1269I GAATGC 2 cut(s) 1349, 4022
MvaI CCWGG 9 cut(s) 456, 2034, 2439, 2499, 2545, 2826, 3491, 3675, 3819
MwoI GCNNNNNNNGC 6 cut(s) 188, 1087, 1127, 1353, 3395, 3823
NarI GGCGCC 1 cut(s) 129
NciI CCSGG 5 cut(s) 37, 114, 971, 3231, 3622
NdeI CATATG 1 cut(s) 4029
NmuCI GTSAC 2 cut(s) 1028, 1322
NsiI ATGCAT 2 cut(s) 1038, 2679
NspI RCATGY 5 cut(s) 1036, 1125, 2120, 2681, 2717
NspV TTCGAA 3 cut(s) 403, 1159, 1341
OliI CACNNNNGTG 1 cut(s) 2238
PaeI GCATGC 1 cut(s) 2717
PaeR7I CTCGAG 1 cut(s) 3081
PagI TCATGA 2 cut(s) 568, 4035
PaqCI CACCTGC 1 cut(s) 1745
PceI AGGCCT 2 cut(s) 454, 3817
PciI ACATGT 1 cut(s) 2116
PciSI GCTCTTC 1 cut(s) 412
PctI GAATGC 2 cut(s) 1349, 4022
PdmI GAANNNNTTC 4 cut(s) 547, 1844, 2492, 2948
PfeI GAWTC 5 cut(s) 373, 1196, 1988, 3125, 3775
PflMI CCANNNNNTGG 1 cut(s) 3073
PfoI TCCNGGA 1 cut(s) 2437
PinAI ACCGGT 3 cut(s) 51, 134, 2581
PkrI GCNGC 5 cut(s) 68, 1080, 2002, 3391, 3742
PleI GAGTC 3 cut(s) 566, 2768, 3758
PluTI GGCGCC 1 cut(s) 132
PpsI GAGTC 3 cut(s) 566, 2768, 3758
PpuMI RGGWCCY 2 cut(s) 620, 1743
PscI ACATGT 1 cut(s) 2116
Psp5II RGGWCCY 2 cut(s) 620, 1743
Psp6I CCWGG 9 cut(s) 454, 2032, 2437, 2497, 2543, 2824, 3489, 3673, 3817
PspFI CCCAGC 1 cut(s) 2444
PspGI CCWGG 9 cut(s) 454, 2032, 2437, 2497, 2543, 2824, 3489, 3673, 3817
PspPI GGNCC 9 cut(s) 386, 620, 1151, 1496, 1743, 1831, 2030, 2651, 3019
PspPPI RGGWCCY 2 cut(s) 620, 1743
PspXI VCTCGAGB 1 cut(s) 3081
PsrI GAACNNNNNNTAC 2 cut(s) 3479, 3511
PsuI RGATCY 8 cut(s) 520, 966, 2182, 2194, 2369, 2571, 3625, 3946
PvuII CAGCTG 1 cut(s) 3740
RsaI GTAC 9 cut(s) 728, 975, 1214, 1273, 1558, 2549, 2866, 2932, 3317
RsaNI GTAC 9 cut(s) 727, 974, 1213, 1272, 1557, 2548, 2865, 2931, 3316
RseI CAYNNNNRTG 3 cut(s) 218, 366, 2238
SapI GCTCTTC 1 cut(s) 412
SaqAI TTAA 8 cut(s) 612, 648, 936, 1362, 1748, 2076, 2294, 2562
SatI GCNGC 5 cut(s) 67, 1079, 2001, 3390, 3741
Sau96I GGNCC 9 cut(s) 386, 620, 1151, 1496, 1743, 1831, 2030, 2651, 3019
ScaI AGTACT 3 cut(s) 728, 1273, 1558
SchI GAGTC 3 cut(s) 566, 2768, 3758
SduI GDGCHC 3 cut(s) 37, 2832, 2853
SexAI ACCWGGT 3 cut(s) 2543, 3489, 3673
SfcI CTRYAG 3 cut(s) 2611, 3236, 3798
SfoI GGCGCC 1 cut(s) 130
Sfr274I CTCGAG 1 cut(s) 3081
SfuI TTCGAA 3 cut(s) 403, 1159, 1341
SinI GGWCC 7 cut(s) 386, 620, 1496, 1743, 1831, 2030, 3019
SlaI CTCGAG 1 cut(s) 3081
SmiMI CAYNNNNRTG 3 cut(s) 218, 366, 2238
SmlI CTYRAG 4 cut(s) 2075, 2805, 3081, 3434
SmoI CTYRAG 4 cut(s) 2075, 2805, 3081, 3434
SphI GCATGC 1 cut(s) 2717
SseBI AGGCCT 2 cut(s) 454, 3817
SsiI CCGC 2 cut(s) 69, 1078
SspDI GGCGCC 1 cut(s) 128
SspI AATATT 3 cut(s) 61, 2362, 2919
SspMI CTAG 8 cut(s) 540, 1647, 2693, 2724, 2834, 2840, 3563, 3747
StuI AGGCCT 2 cut(s) 454, 3817
StyI CCWWGG 5 cut(s) 616, 1468, 3330, 3355, 3371
TaaI ACNGT 7 cut(s) 734, 1224, 1520, 2319, 2600, 3500, 3558
TaiI ACGT 2 cut(s) 2242, 3646
TatI WGTACW 4 cut(s) 726, 1271, 1556, 3315
TauI GCSGC 1 cut(s) 1081
TfiI GAWTC 5 cut(s) 373, 1196, 1988, 3125, 3775
Tru1I TTAA 8 cut(s) 612, 648, 936, 1362, 1748, 2076, 2294, 2562
Tru9I TTAA 8 cut(s) 612, 648, 936, 1362, 1748, 2076, 2294, 2562
TseFI GTSAC 2 cut(s) 1028, 1322
TseI GCWGC 4 cut(s) 66, 2000, 3389, 3740
Tsp45I GTSAC 2 cut(s) 1028, 1322
TspGWI ACGGA 3 cut(s) 903, 1692, 1727
Van91I CCANNNNNTGG 1 cut(s) 3073
Vha464I CTTAAG 1 cut(s) 2075
VpaK11BI GGWCC 7 cut(s) 386, 620, 1496, 1743, 1831, 2030, 3019
XbaI TCTAGA 2 cut(s) 2839, 3562
XceI RCATGY 5 cut(s) 1036, 1125, 2120, 2681, 2717
XcmI CCANNNNNNNNNTGG 1 cut(s) 623
XhoI CTCGAG 1 cut(s) 3081
XmiI GTMKAC 3 cut(s) 5, 2744, 3235
XmnI GAANNNNTTC 4 cut(s) 547, 1844, 2492, 2948
XspI CTAG 8 cut(s) 540, 1647, 2693, 2724, 2834, 2840, 3563, 3747
ZrmI AGTACT 3 cut(s) 728, 1273, 1558
Zsp2I ATGCAT 2 cut(s) 1038, 2679
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.