pycom07g24430

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0013887
Physical Location & Seq
Reverse (-)
25155014 .. 25156001
988 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 900 bp
ATGGCTCCGACGCCGGTGGAGCCGACCGGCGAGTCCACGTCCGGCGAGTCGTCACAGCGAGCTCTTCCAACTCCGTTTTTGACCAAGACGTATCAGCTCGTCGACGACCCCTCAATCGACGACGTCATCTCATGGAACGACGACGGATCCAGCTTCGTCGTCTGGAACCCCACCGTCTTCGCCAGAGATTTGCTCCCCAAGTACTTCAAGCACAATAACTTCTCCAGCTTCGTACGGCAGCTCAACACTTACGGATTTCGGAAGGTTGTGCCGGACCGATGGGAATTCTTGAACGATAGCTTTCGGAGAGGCGGGAAGCGGCTTCTCTGCGAAATACAACGCCGGAAAATCAACCCTCCGGCGCCGCCGGTGGCGTCGGTTTGGGCGGTTCCGACGGCAAAACCGATGATCTCCCCGTCGAACTCCGGCGACGAGCAGGTGATGTCTTCGAGTTCGTCGCCGATCAGAGCATCGTCGGAGATTATGGACGAGAACGAGAGGCTGAGGAAAGAGAACGTGCAGCTCACCAAGGAGCTGGCGGAGGTCAAGTCCCTCTGCAACAACATCTTCACCATGGTATCGAATTACGCTTGCGCCCAATCGGAGAACGGTTTCCCGGAGCTAAAACCGCTGGATTTGATGCCCGGGAAGCGTTTCTCCGGAAAGAGGAAGAACGAAGAGGAAGAAGAGGCGAGTCCGAAGCTTTTCGGGGTGGTGATTGGGGCCAAGCGCGCGAGAGAGGCCGGCGGCGACGGCGTAGAGAACGATGAGACCGACTTGCGGCTTCAGCGACCGAGTGGTAGCGAGGTTAAATCAGAGCCGTCGGATATTGGTCATAAGGAAACGCCGTGGCTGAAGCAATGCCACATGGCTAAGGGTGTGCAACTGATCAGTAGTTAG

Protein Analysis

300

Amino Acids

33.35

Weight (kDa)

6.06

Isoelectric Point (pI)

63.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 26 - 115 2.1e-30 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013887)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G62020
fragaria_vesca FvH4_7g29190
malus_domestica MD01G1198700.v1.1 MD07G1266500.v1.1
prunus_persica Prupe.2G292100_v2.0.a1
pyrus_communis pycom01g20880 pycom07g24430
rosa_chinensis RchiOBHm_Chr1g0376961
rosa_laevigata RLG00000026556
rosa_multiflora Rmu_ssc0000155.1_g000034
rosa_roxburghii Rroxscaffold_4G00281590
rosa_rugosa Rorug01G0399900
rosa_samantha Rh1AG417100 Rh1BG376500 Rh1CG390300 Rh1DG407200
rosa_wichuraiana Rw1G036610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 427
AasI GACNNNNNNGTC 1 cut(s) 31
AatII GACGTC 1 cut(s) 126
Acc36I ACCTGC 1 cut(s) 427
AccB1I GGYRCC 1 cut(s) 361
AccI GTMKAC 1 cut(s) 102
AccII CGCG 2 cut(s) 732, 734
AccIII TCCGGA 1 cut(s) 659
AciI CCGC 8 cut(s) 312, 319, 365, 386, 539, 629, 747, 781
AclWI GGATC 2 cut(s) 141, 154
AcsI RAATTY 1 cut(s) 284
AcuI CTGAAG 2 cut(s) 770, 875
AcyI GRCGYC 4 cut(s) 11, 123, 362, 374
AfaI GTAC 2 cut(s) 203, 234
AfiI CCNNNNNNNGG 2 cut(s) 666, 780
AgsI TTSAA 2 cut(s) 208, 292
AjiI CACGTC 1 cut(s) 39
Alw21I GWGCWC 1 cut(s) 64
Alw26I GTCTC 1 cut(s) 764
AlwI GGATC 2 cut(s) 141, 154
Ama87I CYCGRG 1 cut(s) 644
Aor13HI TCCGGA 1 cut(s) 659
AoxI GGCC 2 cut(s) 723, 741
ApeKI GCWGC 2 cut(s) 238, 520
ApoI RAATTY 1 cut(s) 284
Asp700I GAANNNNTTC 3 cut(s) 611, 653, 704
AspLEI GCGC 4 cut(s) 364, 596, 732, 734
AspS9I GGNCC 2 cut(s) 274, 723
AsuC2I CCSGG 3 cut(s) 617, 645, 646
AsuHPI GGTGA 4 cut(s) 451, 517, 562, 727
AvaI CYCGRG 1 cut(s) 644
AvaII GGWCC 1 cut(s) 274
BaeI ACNNNNGTAYC 2 cut(s) 561, 594
BamHI GGATCC 1 cut(s) 146
BanI GGYRCC 1 cut(s) 361
BanII GRGCYC 1 cut(s) 64
BbsI GAAGAC 2 cut(s) 169, 438
Bbv12I GWGCWC 1 cut(s) 64
BbvCI CCTCAGC 1 cut(s) 503
BbvI GCAGC 2 cut(s) 250, 532
BccI CCATC 1 cut(s) 273
BceAI ACGGC 5 cut(s) 251, 411, 769, 805, 832
BclI TGATCA 1 cut(s) 888
BcnI CCSGG 3 cut(s) 617, 645, 646
BcoDI GTCTC 1 cut(s) 764
BfoI RGCGCY 1 cut(s) 365
BfuAI ACCTGC 1 cut(s) 427
BisI GCNGC 6 cut(s) 239, 320, 365, 521, 748, 782
BlsI GCNGC 6 cut(s) 240, 321, 366, 522, 749, 783
BmcAI AGTACT 1 cut(s) 203
Bme1390I CCNGG 3 cut(s) 617, 645, 646
Bme18I GGWCC 1 cut(s) 274
BmeT110I CYCGRG 1 cut(s) 644
BmgBI CACGTC 1 cut(s) 39
BmgT120I GGNCC 2 cut(s) 274, 723
BmiI GGNNCC 7 cut(s) 6, 21, 148, 167, 363, 390, 724
BmrFI CCNGG 3 cut(s) 617, 645, 646
BmsI GCATC 2 cut(s) 479, 630
BpiI GAAGAC 2 cut(s) 169, 438
BplI GAGNNNNNCTC 2 cut(s) 177, 209
BpmI CTGGAG 1 cut(s) 208
Bpu10I CCTNAGC 2 cut(s) 503, 873
BpuMI CCSGG 3 cut(s) 617, 645, 646
BsaHI GRCGYC 4 cut(s) 11, 123, 362, 374
BsaI GGTCTC 1 cut(s) 764
BsaJI CCNNGG 4 cut(s) 528, 573, 644, 848
BsaWI WCCGGW 1 cut(s) 659
BsaXI ACNNNNNCTCC 2 cut(s) 533, 563
Bsc4I CCNNNNNNNGG 2 cut(s) 666, 780
Bse118I RCCGGY 4 cut(s) 13, 26, 367, 743
Bse3DI GCAATG 1 cut(s) 866
BseAI TCCGGA 1 cut(s) 659
BseDI CCNNGG 4 cut(s) 528, 573, 644, 848
BseLI CCNNNNNNNGG 2 cut(s) 666, 780
BseMI GCAATG 1 cut(s) 866
BseMII CTCAG 1 cut(s) 494
BsePI GCGCGC 1 cut(s) 730
BseXI GCAGC 2 cut(s) 250, 532
BsgI GTGCAG 1 cut(s) 539
Bsh1236I CGCG 2 cut(s) 732, 734
Bsh1285I CGRYCG 2 cut(s) 27, 794
BshFI GGCC 2 cut(s) 725, 743
BshNI GGYRCC 1 cut(s) 361
BsiEI CGRYCG 2 cut(s) 27, 794
BsiHKAI GWGCWC 1 cut(s) 64
BsiHKCI CYCGRG 1 cut(s) 644
BsiWI CGTACG 1 cut(s) 232
BslFI GGGAC 1 cut(s) 535
BslI CCNNNNNNNGG 2 cut(s) 666, 780
BsmAI GTCTC 1 cut(s) 764
BsmFI GGGAC 1 cut(s) 535
BsnI GGCC 2 cut(s) 725, 743
Bso31I GGTCTC 1 cut(s) 764
BsoBI CYCGRG 1 cut(s) 644
Bsp1286I GDGCHC 1 cut(s) 64
Bsp13I TCCGGA 1 cut(s) 659
Bsp143I GATC 4 cut(s) 146, 408, 462, 888
Bsp19I CCATGG 1 cut(s) 573
BspACI CCGC 8 cut(s) 312, 319, 365, 386, 539, 629, 747, 781
BspANI GGCC 2 cut(s) 725, 743
BspCNI CTCAG 1 cut(s) 495
BspEI TCCGGA 1 cut(s) 659
BspFNI CGCG 2 cut(s) 732, 734
BspLI GGNNCC 7 cut(s) 6, 21, 148, 167, 363, 390, 724
BspMI ACCTGC 1 cut(s) 427
BspPI GGATC 2 cut(s) 141, 154
BspQI GCTCTTC 1 cut(s) 69
BspT107I GGYRCC 1 cut(s) 361
BspTNI GGTCTC 1 cut(s) 764
BsrDI GCAATG 1 cut(s) 866
BsrFI RCCGGY 4 cut(s) 13, 26, 367, 743
BssAI RCCGGY 4 cut(s) 13, 26, 367, 743
BssECI CCNNGG 4 cut(s) 528, 573, 644, 848
BssHII GCGCGC 1 cut(s) 730
BssMI GATC 4 cut(s) 146, 408, 462, 888
BssNI GRCGYC 4 cut(s) 11, 123, 362, 374
BssT1I CCWWGG 2 cut(s) 528, 573
Bst4CI ACNGT 2 cut(s) 175, 611
Bst6I CTCTTC 3 cut(s) 69, 672, 681
BstACI GRCGYC 4 cut(s) 11, 123, 362, 374
BstC8I GCNNGC 5 cut(s) 60, 537, 592, 732, 745
BstDEI CTNAG 2 cut(s) 503, 873
BstDSI CCRYGG 2 cut(s) 573, 848
BstFNI CGCG 2 cut(s) 732, 734
BstH2I RGCGCY 1 cut(s) 365
BstHHI GCGC 4 cut(s) 364, 596, 732, 734
BstKTI GATC 4 cut(s) 149, 411, 465, 891
BstMAI GTCTC 1 cut(s) 764
BstMBI GATC 4 cut(s) 146, 408, 462, 888
BstMCI CGRYCG 2 cut(s) 27, 794
BstMWI GCNNNNNNNGC 7 cut(s) 19, 628, 649, 731, 740, 753, 787
BstSCI CCNGG 3 cut(s) 615, 643, 644
BstUI CGCG 2 cut(s) 732, 734
BstV1I GCAGC 2 cut(s) 250, 532
BstV2I GAAGAC 2 cut(s) 169, 438
BstX2I RGATCY 1 cut(s) 146
BstXI CCANNNNNNTGG 1 cut(s) 535
BstYI RGATCY 1 cut(s) 146
BsuRI GGCC 2 cut(s) 725, 743
BtgI CCRYGG 2 cut(s) 573, 848
BtrI CACGTC 1 cut(s) 39
BveI ACCTGC 1 cut(s) 427
Cac8I GCNNGC 5 cut(s) 60, 537, 592, 732, 745
CfoI GCGC 4 cut(s) 364, 596, 732, 734
Cfr10I RCCGGY 4 cut(s) 13, 26, 367, 743
Cfr13I GGNCC 2 cut(s) 274, 723
Cfr9I CCCGGG 1 cut(s) 644
CpoI CGGWCCG 1 cut(s) 274
CseI GACGC 2 cut(s) 19, 363
Csp6I GTAC 2 cut(s) 202, 233
CspI CGGWCCG 1 cut(s) 274
CviAII CATG 3 cut(s) 132, 574, 868
CviQI GTAC 2 cut(s) 202, 233
DdeI CTNAG 2 cut(s) 503, 873
DinI GGCGCC 1 cut(s) 363
DpnI GATC 4 cut(s) 148, 410, 464, 890
DpnII GATC 4 cut(s) 146, 408, 462, 888
DrdI GACNNNNNNGTC 1 cut(s) 31
DseDI GACNNNNNNGTC 1 cut(s) 31
Eam1104I CTCTTC 3 cut(s) 69, 672, 681
EarI CTCTTC 3 cut(s) 69, 672, 681
EciI GGCGGA 1 cut(s) 554
Ecl136II GAGCTC 1 cut(s) 62
Eco130I CCWWGG 2 cut(s) 528, 573
Eco24I GRGCYC 1 cut(s) 64
Eco31I GGTCTC 1 cut(s) 764
Eco47I GGWCC 1 cut(s) 274
Eco53kI GAGCTC 1 cut(s) 62
Eco57I CTGAAG 2 cut(s) 770, 875
Eco88I CYCGRG 1 cut(s) 644
EcoICRI GAGCTC 1 cut(s) 62
EcoRI GAATTC 1 cut(s) 284
EcoT14I CCWWGG 2 cut(s) 528, 573
EcoT38I GRGCYC 1 cut(s) 64
EgeI GGCGCC 1 cut(s) 363
EheI GGCGCC 1 cut(s) 363
ErhI CCWWGG 2 cut(s) 528, 573
FaeI CATG 3 cut(s) 135, 577, 871
FaiI YATR 5 cut(s) 133, 485, 575, 837, 869
FaqI GGGAC 1 cut(s) 535
FatI CATG 3 cut(s) 131, 573, 867
FauI CCCGC 1 cut(s) 305
FbaI TGATCA 1 cut(s) 888
FblI GTMKAC 1 cut(s) 102
Fnu4HI GCNGC 6 cut(s) 239, 320, 365, 521, 748, 782
FriOI GRGCYC 1 cut(s) 64
Fsp4HI GCNGC 6 cut(s) 239, 320, 365, 521, 748, 782
GlaI GCGC 4 cut(s) 363, 595, 731, 733
GluI GCNGC 6 cut(s) 239, 320, 365, 521, 748, 782
GsuI CTGGAG 1 cut(s) 208
HaeII RGCGCY 1 cut(s) 365
HaeIII GGCC 2 cut(s) 725, 743
HgaI GACGC 2 cut(s) 19, 363
HhaI GCGC 4 cut(s) 364, 596, 732, 734
Hin1I GRCGYC 4 cut(s) 11, 123, 362, 374
Hin1II CATG 3 cut(s) 135, 577, 871
Hin6I GCGC 4 cut(s) 362, 594, 730, 732
HinP1I GCGC 4 cut(s) 362, 594, 730, 732
HincII GTYRAC 1 cut(s) 103
HindII GTYRAC 1 cut(s) 103
HindIII AAGCTT 1 cut(s) 701
HinfI GANTC 3 cut(s) 32, 47, 694
HphI GGTGA 4 cut(s) 451, 517, 562, 727
Hpy166II GTNNAC 2 cut(s) 36, 103
Hpy188III TCNNGA 3 cut(s) 163, 289, 660
Hpy8I GTNNAC 2 cut(s) 36, 103
HpyAV CCTTC 1 cut(s) 256
HpyCH4III ACNGT 2 cut(s) 175, 611
HpyCH4IV ACGT 4 cut(s) 38, 89, 123, 516
HpyCH4V TGCA 3 cut(s) 520, 558, 883
HpyF10VI GCNNNNNNNGC 7 cut(s) 19, 628, 649, 731, 740, 753, 787
HpyF3I CTNAG 2 cut(s) 503, 873
HpySE526I ACGT 4 cut(s) 38, 89, 123, 516
Hsp92I GRCGYC 4 cut(s) 11, 123, 362, 374
Hsp92II CATG 3 cut(s) 135, 577, 871
HspAI GCGC 4 cut(s) 362, 594, 730, 732
KasI GGCGCC 1 cut(s) 361
Kpn2I TCCGGA 1 cut(s) 659
KroI GCCGGC 1 cut(s) 743
KroNI GCCGGC 1 cut(s) 745
Ksp22I TGATCA 1 cut(s) 888
Kzo9I GATC 4 cut(s) 146, 408, 462, 888
LguI GCTCTTC 1 cut(s) 69
LmnI GCTCC 5 cut(s) 10, 19, 198, 532, 619
Lsp1109I GCAGC 2 cut(s) 250, 532
LweI GCATC 2 cut(s) 479, 630
MaeII ACGT 4 cut(s) 38, 89, 123, 516
MaeIII GTNAC 1 cut(s) 51
MalI GATC 4 cut(s) 148, 410, 464, 890
MboI GATC 4 cut(s) 146, 408, 462, 888
MboII GAAGA 8 cut(s) 56, 169, 438, 559, 682, 689, 695, 698
MflI RGATCY 1 cut(s) 146
MhlI GDGCHC 1 cut(s) 64
MluCI AATT 2 cut(s) 284, 583
Mly113I GGCGCC 1 cut(s) 362
MlyI GAGTC 3 cut(s) 41, 56, 703
MmeI TCCRAC 5 cut(s) 32, 92, 416, 456, 804
MroI TCCGGA 1 cut(s) 659
MroNI GCCGGC 1 cut(s) 743
MroXI GAANNNNTTC 3 cut(s) 611, 653, 704
MseI TTAA 1 cut(s) 810
MspA1I CMGCKG 1 cut(s) 631
MspR9I CCNGG 3 cut(s) 617, 645, 646
MvnI CGCG 2 cut(s) 732, 734
MwoI GCNNNNNNNGC 7 cut(s) 19, 628, 649, 731, 740, 753, 787
NaeI GCCGGC 1 cut(s) 745
NarI GGCGCC 1 cut(s) 362
NciI CCSGG 3 cut(s) 617, 645, 646
NcoI CCATGG 1 cut(s) 573
NdeII GATC 4 cut(s) 146, 408, 462, 888
NgoMIV GCCGGC 1 cut(s) 743
NlaIII CATG 3 cut(s) 135, 577, 871
NlaIV GGNNCC 7 cut(s) 6, 21, 148, 167, 363, 390, 724
NmuCI GTSAC 1 cut(s) 51
PaqCI CACCTGC 1 cut(s) 427
PauI GCGCGC 1 cut(s) 730
PciSI GCTCTTC 1 cut(s) 69
PcsI WCGNNNNNNNCGW 2 cut(s) 401, 771
PdiI GCCGGC 1 cut(s) 745
PdmI GAANNNNTTC 3 cut(s) 611, 653, 704
Pfl23II CGTACG 1 cut(s) 232
PflFI GACNNNGTC 1 cut(s) 122
PfoI TCCNGGA 1 cut(s) 615
PkrI GCNGC 6 cut(s) 240, 321, 366, 522, 749, 783
PleI GAGTC 3 cut(s) 40, 55, 702
PluTI GGCGCC 1 cut(s) 365
PpsI GAGTC 3 cut(s) 40, 55, 702
Psp124BI GAGCTC 1 cut(s) 64
PspLI CGTACG 1 cut(s) 232
PspN4I GGNNCC 7 cut(s) 6, 21, 148, 167, 363, 390, 724
PspPI GGNCC 2 cut(s) 274, 723
PsuI RGATCY 1 cut(s) 146
PsyI GACNNNGTC 1 cut(s) 122
PteI GCGCGC 1 cut(s) 730
RsaI GTAC 2 cut(s) 203, 234
RsaNI GTAC 2 cut(s) 202, 233
Rsr2I CGGWCCG 1 cut(s) 274
RsrII CGGWCCG 1 cut(s) 274
SacI GAGCTC 1 cut(s) 64
SalI GTCGAC 1 cut(s) 101
SapI GCTCTTC 1 cut(s) 69
SaqAI TTAA 1 cut(s) 810
SatI GCNGC 6 cut(s) 239, 320, 365, 521, 748, 782
Sau3AI GATC 4 cut(s) 146, 408, 462, 888
Sau96I GGNCC 2 cut(s) 274, 723
ScaI AGTACT 1 cut(s) 203
SchI GAGTC 3 cut(s) 41, 56, 703
ScrFI CCNGG 3 cut(s) 617, 645, 646
SduI GDGCHC 1 cut(s) 64
SfaNI GCATC 2 cut(s) 479, 630
SfoI GGCGCC 1 cut(s) 363
SgrAI CRCCGGYG 2 cut(s) 13, 367
SgrDI CGTCGACG 1 cut(s) 101
SinI GGWCC 1 cut(s) 274
SmaI CCCGGG 1 cut(s) 646
Sse9I AATT 2 cut(s) 284, 583
SsiI CCGC 8 cut(s) 312, 319, 365, 386, 539, 629, 747, 781
SspDI GGCGCC 1 cut(s) 361
SstI GAGCTC 1 cut(s) 64
StyD4I CCNGG 3 cut(s) 615, 643, 644
StyI CCWWGG 2 cut(s) 528, 573
TaaI ACNGT 2 cut(s) 175, 611
TaiI ACGT 4 cut(s) 41, 92, 126, 519
TaqI TCGA 5 cut(s) 102, 117, 419, 449, 581
TaqII GACCGA 3 cut(s) 291, 788, 808
TasI AATT 2 cut(s) 284, 583
TatI WGTACW 1 cut(s) 201
TauI GCSGC 4 cut(s) 322, 367, 750, 784
Tru1I TTAA 1 cut(s) 810
Tru9I TTAA 1 cut(s) 810
TseFI GTSAC 1 cut(s) 51
TseI GCWGC 2 cut(s) 238, 520
Tsp45I GTSAC 1 cut(s) 51
TspGWI ACGGA 3 cut(s) 63, 159, 267
TspMI CCCGGG 1 cut(s) 644
Tth111I GACNNNGTC 1 cut(s) 122
VpaK11BI GGWCC 1 cut(s) 274
XapI RAATTY 1 cut(s) 284
XmaI CCCGGG 1 cut(s) 644
XmiI GTMKAC 1 cut(s) 102
XmnI GAANNNNTTC 3 cut(s) 611, 653, 704
ZraI GACGTC 1 cut(s) 124
ZrmI AGTACT 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.