pycom07g26710

acyl-CoA-binding domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0007027
Physical Location & Seq
Reverse (-)
26661987 .. 26664515
2529 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1071 bp
ATGGGCGGAGAGTGGCAGCAGCTCCTCCAATCGATAATCCTCGGACTCATTTTCTCCTACCTCCTCGCCAAGCTCATCGCCATAGTCATCTCCTTCAAGGAAGAGAATCTCAGCCTCACTCGCGCTCGCGCTGCCGCCCCCGCCGACACCAATCCGCCGCCGCTCAAATCCGAGCCCCGCGACGACCAGCTTCGCTCCGCCGATTTGAGCTCCGCGGCAATTGAGGCCGAATCGGTCGTCGCCGAGCACGGCAGCGTCAGGAACGATAGCAGCGAAGCTGACAGTGACGATGACTGGGAAGGCGTGGAGAGTACGGAATTGGACGAGCTGTTCAGCGCGGCGACGGCGTTTGTCGCGGCGGCTGCGGTGGATCGACAGAAGGTGTCGAGTGATGCGCAGTTGAAGCTTTATGGGCTTTACAAGATTGCTACTGAGGGACCCTGCAGCGCGCCGCAGCCATCTGCTCTGAAAATGACTGCTCGCGCGAAATGGCAAGCTTGGCAGAAATTGGGTGCTATGCCTCCTGAAGATGCTATGGAGAAGTACATTGATATTGTCACAGAGCTTTATCCTAATTGGGCAGCAGGTCTGACTGTGAAGAGCAGAGGTGGAGAAGGTAGTGCATCTGGTAATGATTCTAAAGGACCAATGGGACCGGTTTTCAGCACCTTTGTTTACGAGGAAGAATCTGAAAAGGATTTAAAGATGGATGCTATTCATGCCTTTGCGAGAGAAGGAGAAGTGGATAATTTGCTTAAATGCATTGAAAGTGGCGTTTCGGTGGATCAGAAAGATAGTGAGGGAAGGACCCCATTGCATTGGGCTGTGGATCGTGGGCACCTGAACATGACAGAGCTGCTAGTTAGCAAGAATGCCAATGTTAATGAAAAGGACAATGAGGGGCAAACCGCATTGCATTACGCTGTTATGTGTGATAGAGAAGGCATTGCTGAATTTCTTGTGAAGCAAAATGCAGACACAAGTGCAAAGGACAACGATGGCAACTCTCCCTGTGATGTCTGCGAGTCAAACTGGCCTTGGTTTCAGGGTGCAAAGCAGAAGAGTCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000062 GO:0000166 GO:0001101 GO:0001666 GO:0003006 GO:0003674 GO:0005488 GO:0005543 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005778 GO:0005783 GO:0005789 GO:0005886 GO:0006629 GO:0006810 GO:0006869 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0008289 GO:0009266 GO:0009409 GO:0009505 GO:0009514 GO:0009628 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0010029 GO:0010033 GO:0010035 GO:0010038 GO:0010154 GO:0010162 GO:0010288 GO:0010431 GO:0010876 GO:0012505 GO:0016020 GO:0017076 GO:0021700 GO:0022414 GO:0022611 GO:0030312 GO:0030554 GO:0031090 GO:0031903 GO:0031984 GO:0032501 GO:0032502 GO:0032504 GO:0032553 GO:0032555 GO:0032559 GO:0032791 GO:0033036 GO:0033218 GO:0033993 GO:0036094 GO:0036293 GO:0042175 GO:0042221 GO:0042579 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0048037 GO:0048316 GO:0048580 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050662 GO:0050789 GO:0050793 GO:0050896 GO:0051179 GO:0051234 GO:0051239 GO:0061458 GO:0065007 GO:0070300 GO:0070482 GO:0071695 GO:0071702 GO:0071704 GO:0071944 GO:0097159 GO:0097305 GO:0097367 GO:0098588 GO:0098805 GO:0098827 GO:1900140 GO:1901265 GO:1901363 GO:1901567 GO:1901681 GO:1901700 GO:2000026
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACBP Ank_2 Ank_4 Ank_5

Protein Analysis

357

Amino Acids

38.73

Weight (kDa)

4.62

Isoelectric Point (pI)

34.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACBP PF00887 108 - 186 5.6e-27 Acyl CoA binding protein
Ank_4 PF13637 239 - 288 2.1e-08 Ankyrin repeats (many copies)
Ank_2 PF12796 240 - 331 6.4e-19 Ankyrin repeats (3 copies)
Ank_5 PF13857 259 - 308 1.9e-09 Ankyrin repeats (many copies)
Ank PF00023 268 - 299 4e-09 Ankyrin repeat
Ank_4 PF13637 272 - 321 5.2e-11 Ankyrin repeats (many copies)
Ank_5 PF13857 291 - 340 1.8e-06 Ankyrin repeats (many copies)
Ank PF00023 301 - 332 4.2e-06 Ankyrin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 396
Acc36I ACCTGC 1 cut(s) 575
AccB1I GGYRCC 1 cut(s) 837
AccBSI CCGCTC 1 cut(s) 163
AccII CGCG 9 cut(s) 123, 129, 180, 215, 338, 356, 449, 483, 485
AclWI GGATC 3 cut(s) 378, 792, 837
AcsI RAATTY 1 cut(s) 953
AcuI CTGAAG 1 cut(s) 546
AfaI GTAC 2 cut(s) 313, 545
AgeI ACCGGT 1 cut(s) 655
AgsI TTSAA 3 cut(s) 97, 403, 767
AloI GAACNNNNNNTCC 2 cut(s) 314, 346
Alw21I GWGCWC 2 cut(s) 212, 249
AlwI GGATC 3 cut(s) 378, 792, 837
AoxI GGCC 2 cut(s) 225, 1034
ApoI RAATTY 1 cut(s) 953
ArsI GACNNNNNNTTYG 2 cut(s) 222, 254
AsiGI ACCGGT 1 cut(s) 655
AspLEI GCGC 7 cut(s) 125, 131, 338, 397, 449, 451, 485
AspS9I GGNCC 4 cut(s) 437, 644, 653, 807
AvaII GGWCC 4 cut(s) 437, 644, 653, 807
BaeGI GKGCMC 1 cut(s) 840
BanI GGYRCC 1 cut(s) 837
BanII GRGCYC 2 cut(s) 177, 212
Bbv12I GWGCWC 2 cut(s) 212, 249
BccI CCATC 3 cut(s) 466, 700, 992
BceAI ACGGC 2 cut(s) 265, 360
BfaI CTAG 1 cut(s) 860
BfmI CTRYAG 1 cut(s) 442
BfuAI ACCTGC 1 cut(s) 575
Bme18I GGWCC 4 cut(s) 437, 644, 653, 807
BmgT120I GGNCC 4 cut(s) 437, 644, 653, 807
BmiI GGNNCC 5 cut(s) 438, 439, 654, 809, 839
BmrI ACTGGG 1 cut(s) 304
BmsI GCATC 4 cut(s) 382, 520, 632, 700
BmuI ACTGGG 1 cut(s) 304
Bsa29I ATCGAT 1 cut(s) 32
BsaJI CCNNGG 3 cut(s) 40, 213, 1037
BsaWI WCCGGW 1 cut(s) 655
Bse118I RCCGGY 1 cut(s) 655
Bse1I ACTGG 2 cut(s) 299, 1037
Bse3DI GCAATG 3 cut(s) 812, 911, 945
BseCI ATCGAT 1 cut(s) 32
BseDI CCNNGG 3 cut(s) 40, 213, 1037
BseGI GGATG 1 cut(s) 715
BseMI GCAATG 3 cut(s) 812, 911, 945
BseMII CTCAG 2 cut(s) 124, 423
BseNI ACTGG 2 cut(s) 299, 1037
BsePI GCGCGC 1 cut(s) 447
BseRI GAGGAG 2 cut(s) 14, 53
BseSI GKGCMC 1 cut(s) 840
Bsh1236I CGCG 9 cut(s) 123, 129, 180, 215, 338, 356, 449, 483, 485
Bsh1285I CGRYCG 1 cut(s) 237
BshFI GGCC 2 cut(s) 227, 1036
BshNI GGYRCC 1 cut(s) 837
BshTI ACCGGT 1 cut(s) 655
BshVI ATCGAT 1 cut(s) 32
BsiEI CGRYCG 1 cut(s) 237
BsiHKAI GWGCWC 2 cut(s) 212, 249
BsiSI CCGG 1 cut(s) 656
BslFI GGGAC 2 cut(s) 450, 666
BsmFI GGGAC 2 cut(s) 450, 666
BsmI GAATGC 1 cut(s) 877
BsnI GGCC 2 cut(s) 227, 1036
Bsp1286I GDGCHC 4 cut(s) 177, 212, 249, 840
Bsp143I GATC 3 cut(s) 370, 784, 829
BspANI GGCC 2 cut(s) 227, 1036
BspCNI CTCAG 2 cut(s) 123, 424
BspDI ATCGAT 1 cut(s) 32
BspFNI CGCG 9 cut(s) 123, 129, 180, 215, 338, 356, 449, 483, 485
BspLI GGNNCC 5 cut(s) 438, 439, 654, 809, 839
BspMAI CTGCAG 1 cut(s) 446
BspMI ACCTGC 1 cut(s) 575
BspPI GGATC 3 cut(s) 378, 792, 837
BspQI GCTCTTC 1 cut(s) 593
BspT107I GGYRCC 1 cut(s) 837
BsrBI CCGCTC 1 cut(s) 163
BsrDI GCAATG 3 cut(s) 812, 911, 945
BsrFI RCCGGY 1 cut(s) 655
BsrI ACTGG 2 cut(s) 299, 1037
BssAI RCCGGY 1 cut(s) 655
BssECI CCNNGG 3 cut(s) 40, 213, 1037
BssHII GCGCGC 1 cut(s) 447
BssMI GATC 3 cut(s) 370, 784, 829
BssT1I CCWWGG 1 cut(s) 1037
Bst4CI ACNGT 2 cut(s) 284, 595
Bst6I CTCTTC 3 cut(s) 96, 593, 1055
BstC8I GCNNGC 4 cut(s) 127, 449, 481, 495
BstDEI CTNAG 2 cut(s) 110, 432
BstDSI CCRYGG 1 cut(s) 213
BstF5I GGATG 1 cut(s) 715
BstFNI CGCG 9 cut(s) 123, 129, 180, 215, 338, 356, 449, 483, 485
BstHHI GCGC 7 cut(s) 125, 131, 338, 397, 449, 451, 485
BstKTI GATC 3 cut(s) 373, 787, 832
BstMBI GATC 3 cut(s) 370, 784, 829
BstMCI CGRYCG 1 cut(s) 237
BstSFI CTRYAG 1 cut(s) 442
BstSLI GKGCMC 1 cut(s) 840
BstUI CGCG 9 cut(s) 123, 129, 180, 215, 338, 356, 449, 483, 485
BstXI CCANNNNNNTGG 1 cut(s) 819
Bsu15I ATCGAT 1 cut(s) 32
BsuRI GGCC 2 cut(s) 227, 1036
BsuTUI ATCGAT 1 cut(s) 32
BtgI CCRYGG 1 cut(s) 213
BtgZI GCGATG 1 cut(s) 61
BtsCI GGATG 1 cut(s) 715
BtsIMutI CAGTG 2 cut(s) 289, 1066
BveI ACCTGC 1 cut(s) 575
Cac8I GCNNGC 4 cut(s) 127, 449, 481, 495
CfoI GCGC 7 cut(s) 125, 131, 338, 397, 449, 451, 485
Cfr10I RCCGGY 1 cut(s) 655
Cfr13I GGNCC 4 cut(s) 437, 644, 653, 807
Cfr42I CCGCGG 1 cut(s) 216
ClaI ATCGAT 1 cut(s) 32
CseI GACGC 1 cut(s) 244
Csp6I GTAC 2 cut(s) 312, 544
CspAI ACCGGT 1 cut(s) 655
CviAII CATG 2 cut(s) 719, 847
CviQI GTAC 2 cut(s) 312, 544
DdeI CTNAG 2 cut(s) 110, 432
DpnI GATC 3 cut(s) 372, 786, 831
DpnII GATC 3 cut(s) 370, 784, 829
DraI TTTAAA 1 cut(s) 702
Eam1104I CTCTTC 3 cut(s) 96, 593, 1055
EarI CTCTTC 3 cut(s) 96, 593, 1055
EciI GGCGGA 3 cut(s) 21, 144, 187
Ecl136II GAGCTC 1 cut(s) 210
Eco130I CCWWGG 1 cut(s) 1037
Eco24I GRGCYC 2 cut(s) 177, 212
Eco47I GGWCC 4 cut(s) 437, 644, 653, 807
Eco53kI GAGCTC 1 cut(s) 210
Eco57I CTGAAG 1 cut(s) 546
EcoICRI GAGCTC 1 cut(s) 210
EcoO109I RGGNCCY 2 cut(s) 437, 807
EcoT14I CCWWGG 1 cut(s) 1037
EcoT22I ATGCAT 1 cut(s) 764
EcoT38I GRGCYC 2 cut(s) 177, 212
ErhI CCWWGG 1 cut(s) 1037
FaeI CATG 2 cut(s) 722, 850
FaiI YATR 7 cut(s) 83, 411, 518, 536, 720, 848, 929
FaqI GGGAC 2 cut(s) 450, 666
FatI CATG 2 cut(s) 718, 846
FauI CCCGC 2 cut(s) 148, 185
FokI GGATG 1 cut(s) 722
FriOI GRGCYC 2 cut(s) 177, 212
FspBI CTAG 1 cut(s) 860
FspI TGCGCA 1 cut(s) 396
GlaI GCGC 7 cut(s) 124, 130, 337, 396, 448, 450, 484
HaeIII GGCC 2 cut(s) 227, 1036
HapII CCGG 1 cut(s) 656
HgaI GACGC 1 cut(s) 244
HhaI GCGC 7 cut(s) 125, 131, 338, 397, 449, 451, 485
Hin1II CATG 2 cut(s) 722, 850
Hin6I GCGC 7 cut(s) 123, 129, 336, 395, 447, 449, 483
HinP1I GCGC 7 cut(s) 123, 129, 336, 395, 447, 449, 483
HindIII AAGCTT 2 cut(s) 404, 495
HinfI GANTC 7 cut(s) 45, 106, 230, 635, 686, 1025, 1063
HpaII CCGG 1 cut(s) 656
Hpy166II GTNNAC 1 cut(s) 676
Hpy188I TCNGA 6 cut(s) 44, 172, 468, 591, 691, 789
Hpy188III TCNNGA 2 cut(s) 259, 524
Hpy8I GTNNAC 1 cut(s) 676
Hpy99I CGWCG 3 cut(s) 185, 242, 346
HpyAV CCTTC 7 cut(s) 103, 293, 373, 608, 728, 798, 935
HpyCH4III ACNGT 2 cut(s) 284, 595
HpyCH4V TGCA 8 cut(s) 444, 623, 762, 817, 916, 974, 986, 1052
HpyF3I CTNAG 2 cut(s) 110, 432
Hsp92II CATG 2 cut(s) 722, 850
HspAI GCGC 7 cut(s) 123, 129, 336, 395, 447, 449, 483
KflI GGGWCCC 1 cut(s) 437
KspI CCGCGG 1 cut(s) 216
Kzo9I GATC 3 cut(s) 370, 784, 829
LguI GCTCTTC 1 cut(s) 593
LmnI GCTCC 3 cut(s) 27, 200, 215
LweI GCATC 4 cut(s) 382, 520, 632, 700
MaeI CTAG 1 cut(s) 860
MaeIII GTNAC 3 cut(s) 284, 556, 1064
MalI GATC 3 cut(s) 372, 786, 831
MbiI CCGCTC 1 cut(s) 163
MboI GATC 3 cut(s) 370, 784, 829
MboII GAAGA 4 cut(s) 113, 539, 610, 695
MfeI CAATTG 1 cut(s) 219
MhlI GDGCHC 4 cut(s) 177, 212, 249, 840
MluCI AATT 6 cut(s) 219, 317, 506, 574, 748, 953
MlyI GAGTC 2 cut(s) 39, 1034
Mph1103I ATGCAT 1 cut(s) 764
MseI TTAA 3 cut(s) 701, 756, 882
MspA1I CMGCKG 1 cut(s) 215
MspI CCGG 1 cut(s) 656
MunI CAATTG 1 cut(s) 219
Mva1269I GAATGC 1 cut(s) 877
MvnI CGCG 9 cut(s) 123, 129, 180, 215, 338, 356, 449, 483, 485
NdeII GATC 3 cut(s) 370, 784, 829
NlaIII CATG 2 cut(s) 722, 850
NlaIV GGNNCC 5 cut(s) 438, 439, 654, 809, 839
NmeAIII GCCGAG 1 cut(s) 268
NmuCI GTSAC 3 cut(s) 284, 556, 1064
NsbI TGCGCA 1 cut(s) 396
NsiI ATGCAT 1 cut(s) 764
PauI GCGCGC 1 cut(s) 447
PciSI GCTCTTC 1 cut(s) 593
PcsI WCGNNNNNNNCGW 1 cut(s) 270
PctI GAATGC 1 cut(s) 877
PfeI GAWTC 4 cut(s) 106, 230, 635, 686
PinAI ACCGGT 1 cut(s) 655
PleI GAGTC 2 cut(s) 39, 1033
PpsI GAGTC 2 cut(s) 39, 1033
PpuMI RGGWCCY 2 cut(s) 437, 807
Psp124BI GAGCTC 1 cut(s) 212
Psp5II RGGWCCY 2 cut(s) 437, 807
PspN4I GGNNCC 5 cut(s) 438, 439, 654, 809, 839
PspPI GGNCC 4 cut(s) 437, 644, 653, 807
PspPPI RGGWCCY 2 cut(s) 437, 807
PstI CTGCAG 1 cut(s) 446
PteI GCGCGC 1 cut(s) 447
RsaI GTAC 2 cut(s) 313, 545
RsaNI GTAC 2 cut(s) 312, 544
SacI GAGCTC 1 cut(s) 212
SacII CCGCGG 1 cut(s) 216
SapI GCTCTTC 1 cut(s) 593
SaqAI TTAA 3 cut(s) 701, 756, 882
Sau3AI GATC 3 cut(s) 370, 784, 829
Sau96I GGNCC 4 cut(s) 437, 644, 653, 807
SchI GAGTC 2 cut(s) 39, 1034
SduI GDGCHC 4 cut(s) 177, 212, 249, 840
SfaNI GCATC 4 cut(s) 382, 520, 632, 700
SfcI CTRYAG 1 cut(s) 442
Sfr303I CCGCGG 1 cut(s) 216
SgrBI CCGCGG 1 cut(s) 216
SinI GGWCC 4 cut(s) 437, 644, 653, 807
Sse9I AATT 6 cut(s) 219, 317, 506, 574, 748, 953
SspMI CTAG 1 cut(s) 860
SstI GAGCTC 1 cut(s) 212
StyI CCWWGG 1 cut(s) 1037
TaaI ACNGT 2 cut(s) 284, 595
TaqI TCGA 3 cut(s) 32, 373, 386
TaqII GACCGA 1 cut(s) 223
TasI AATT 6 cut(s) 219, 317, 506, 574, 748, 953
TatI WGTACW 1 cut(s) 543
TauI GCSGC 8 cut(s) 137, 160, 163, 218, 341, 359, 362, 454
TfiI GAWTC 4 cut(s) 106, 230, 635, 686
Tru1I TTAA 3 cut(s) 701, 756, 882
Tru9I TTAA 3 cut(s) 701, 756, 882
TscAI CASTG 1 cut(s) 289
TseFI GTSAC 3 cut(s) 284, 556, 1064
Tsp45I GTSAC 3 cut(s) 284, 556, 1064
TspDTI ATGAA 2 cut(s) 707, 900
TspGWI ACGGA 1 cut(s) 329
TspRI CASTG 1 cut(s) 289
VpaK11BI GGWCC 4 cut(s) 437, 644, 653, 807
XapI RAATTY 1 cut(s) 953
XspI CTAG 1 cut(s) 860
Zsp2I ATGCAT 1 cut(s) 764
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.