pycom08g00080

Zinc finger CCHC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0012293
Physical Location & Seq
Forward (+)
101387 .. 104949
3563 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g00080.5

Sequence Viewer

Length: 1629 bp
ATGGAAGGTGAAGAGGAAGGAGGGATAAGGCTGAGTAAGAGATTTGCGGACAAAGGAGGAGGAGGAGGAGGAGGTGGGGAGGTTGATTACAAGAATAAGTCAGGCACGGCGTGGAGCCACTCGTACCTGAATCAGAAGCCATGGCACCCTCTCTCTTACCCCAATCAGCGCCGTAAGTGGATCGCAGAGCAGATCCAGGCCCAGCGTCAGCGTCGTACCGAGGAGATTGAGACTATGAAAGCTGTTAGCTTTATGTATGTTCGACCGCCTGGTTACAATGCCGAAAGTGCAAAAGCTGCAGAGATTGCTGATGAGAGTGCAAGAGAGCACCCACAAACACAAGCTCTAACAGATGCTACTGCTACTTCCACTTCAAGCCAACGCCCACCCGAGTCTTTGCCTCCCACTGCTGAACATGCCAAGAAATCAAGGCCTAAAGATGTGTTTGGCCGTCCTTTACCAACAGAACAAGAATTTGAAGTTTTGAAAAATGCTCCAAGGCTGGAGACTGGTGTTCCTACGAGGGCAAAACCATTTGGAGTTGAAGTGCGCAATGTAAAGTGCGTAAGGTGTGGAACTTTTGGTCACCAAAGTGGCGACCGTGAATGTCCGTTGAAGGATGTCATAATGCCCAACGAAGAGAGCCGTTTGAAAAGGGACGACCCTTTGACTGCTATTCTTGCCCACACAGATCCTAGCGAGCCTTTAAAGTGGGAACTCAAGCAAAAACCAGGAATTAGTCCTCCACGTGGAGGGTTTAAACCAGATGATCCCAACCAACAAATAGTTGCTGAGGACACCATATTTGATGAATATGGAGGGTTTCTCAGCGGGAATGCCATCCCTGAGTTGCTGACCAACTTCTCAAGCAAACCCAGGTTCAAGTCCACAAAGAAGAACAAGCACAAAAAAAAGCAGTCATCACCAGTTAGTGGGGAAGATGGGTTTTCATCTTCTTATGAGGATGATAAGAAGTCAAAGAAGAAGATGAGCAAGGTAAATAAGAAGAAGCACAGCCATTCTGAATCAAGCTCATCAGAGATTCTGGAATTTGATAGGCATAAAGTAAAGCGTAGAGTCAAGCATTGTTATTCATGTGAGAAATCAAATCATGAAAAGCATCACAAAAGTATAAAGAACAGAAAGAAGCATTCTGATTCATCTGAAAATTCCGAGATTTATAGGCATTACAGCAGAAGGGACAACAGCAGTGTCAGGTGTACTGTTTCATCTGAAGATTCTGAGGCTCTGAAGCACCATAGAGATGTCCAGCGCAGACGCAAGCACTCTTTTTCATTTGAGGAATCGGTTCATGAAAAACATCGTAGAAGTAGAATGATCAGACAGAAGCATTCTCATTCATATGAAGATTCCAAGATTGATAGGCATTGCGGAAGGGACGTGAGCAGGGACAGGTTAAATAATTCATCTAAAGGCTCTGAGTCTGATAGACCCCTTAAAAGTGTCAAAAGCAGACACCGGCATTCTAATTCATCTGAAGATTCTGACACAGAGAAGGAAAATAGACATAGAAAGAGCAGGGATAGGTACTCTTACTCATATGGTGAAACCGAAAATGAGAAGCATCATAAAAGTACTAACAGAAAACACAAGCGCTTTTGTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04330 map04330
Pfam Domains
Protein Families
Cir_N zf-CCHC_6

Protein Analysis

543

Amino Acids

62.27

Weight (kDa)

9.63

Isoelectric Point (pI)

62.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012293)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G19190
fragaria_vesca FvH4_2g37380 FvH4_3g03932
malus_domestica MD08G1001400.v1.1
prunus_persica Prupe.1G353400_v2.0.a1 Prupe.1G353400_v2.0.a1
pyrus_communis pycom08g00080
rosa_chinensis RchiOBHm_Chr6g0304321
rosa_laevigata RLG00000010990
rosa_multiflora Rmu_co8215432.1_g000001 Rmu_ssc0000218.1_g000039
rosa_roxburghii Rroxscaffold_7G00163840
rosa_rugosa Rorug06G0330100 Rorug06G0330200
rosa_samantha Rh6AG443400 Rh6BG459000 Rh6CG456200 Rh6DG442900
rosa_wichuraiana Rw6G038480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 551
AccB1I GGYRCC 1 cut(s) 144
AccB7I CCANNNNNTGG 1 cut(s) 932
AciI CCGC 4 cut(s) 47, 266, 831, 1392
AclWI GGATC 4 cut(s) 187, 188, 686, 764
AcoI YGGCCR 1 cut(s) 448
AcsI RAATTY 3 cut(s) 473, 1049, 1168
AcuI CTGAAG 3 cut(s) 1254, 1271, 1518
AcvI CACGTG 1 cut(s) 749
AdeI CACNNNGTG 1 cut(s) 111
AfaI GTAC 6 cut(s) 125, 217, 1222, 1550, 1597, 1624
AfeI AGCGCT 1 cut(s) 1616
AfiI CCNNNNNNNGG 3 cut(s) 749, 752, 932
AgsI TTSAA 7 cut(s) 375, 479, 487, 545, 616, 652, 883
AjiI CACGTC 1 cut(s) 1402
AjnI CCWGG 4 cut(s) 195, 268, 730, 875
AleI CACNNNNGTG 1 cut(s) 591
AluBI AGCT 5 cut(s) 242, 249, 296, 344, 1032
AluI AGCT 5 cut(s) 242, 249, 296, 344, 1032
Alw21I GWGCWC 1 cut(s) 330
Alw26I GTCTC 2 cut(s) 224, 500
AlwI GGATC 4 cut(s) 187, 188, 686, 764
Ama87I CYCGRG 1 cut(s) 389
Aor51HI AGCGCT 1 cut(s) 1616
AoxI GGCC 3 cut(s) 198, 431, 448
ApeKI GCWGC 1 cut(s) 296
ApoI RAATTY 3 cut(s) 473, 1049, 1168
ArsI GACNNNNNNTTYG 2 cut(s) 788, 820
Asp700I GAANNNNTTC 1 cut(s) 1308
AspLEI GCGC 4 cut(s) 171, 552, 1275, 1617
AspS9I GGNCC 1 cut(s) 199
AsuHPI GGTGA 4 cut(s) 20, 578, 915, 1577
AvaI CYCGRG 1 cut(s) 389
BanI GGYRCC 1 cut(s) 144
BbrPI CACGTG 1 cut(s) 749
Bbv12I GWGCWC 1 cut(s) 330
BbvCI CCTCAGC 1 cut(s) 792
BbvI GCAGC 1 cut(s) 283
BccI CCATC 2 cut(s) 848, 935
BceAI ACGGC 4 cut(s) 123, 156, 435, 630
BciT130I CCWGG 4 cut(s) 197, 270, 732, 877
BclI TGATCA 1 cut(s) 1338
BcoDI GTCTC 2 cut(s) 224, 500
BfaI CTAG 1 cut(s) 696
BfmI CTRYAG 1 cut(s) 297
BfoI RGCGCY 2 cut(s) 172, 1618
BisI GCNGC 1 cut(s) 297
BlsI GCNGC 1 cut(s) 298
BmcAI AGTACT 1 cut(s) 1597
Bme1390I CCNGG 4 cut(s) 197, 270, 732, 877
BmeT110I CYCGRG 1 cut(s) 389
BmgBI CACGTC 1 cut(s) 1402
BmgT120I GGNCC 1 cut(s) 199
BmiI GGNNCC 2 cut(s) 116, 146
BmrFI CCNGG 4 cut(s) 197, 270, 732, 877
BmsI GCATC 3 cut(s) 343, 1129, 1594
BplI GAGNNNNNCTC 2 cut(s) 810, 842
BpmI CTGGAG 1 cut(s) 524
Bpu10I CCTNAGC 1 cut(s) 792
BpuEI CTTGAG 2 cut(s) 704, 850
BsaAI YACGTR 1 cut(s) 749
BsaJI CCNNGG 4 cut(s) 140, 219, 497, 875
BsaXI ACNNNNNCTCC 2 cut(s) 531, 561
Bsc4I CCNNNNNNNGG 3 cut(s) 749, 752, 932
Bse118I RCCGGY 1 cut(s) 1479
Bse1I ACTGG 2 cut(s) 514, 926
Bse3DI GCAATG 2 cut(s) 559, 1387
BseBI CCWGG 4 cut(s) 197, 270, 732, 877
BseDI CCNNGG 4 cut(s) 140, 219, 497, 875
BseGI GGATG 3 cut(s) 625, 840, 970
BseLI CCNNNNNNNGG 3 cut(s) 749, 752, 932
BseMI GCAATG 2 cut(s) 559, 1387
BseMII CTCAG 6 cut(s) 23, 783, 837, 841, 1233, 1431
BseNI ACTGG 2 cut(s) 514, 926
BseRI GAGGAG 6 cut(s) 72, 75, 78, 81, 84, 236
BseXI GCAGC 1 cut(s) 283
BseYI CCCAGC 1 cut(s) 201
Bsh1285I CGRYCG 2 cut(s) 266, 601
BshFI GGCC 3 cut(s) 200, 433, 450
BshNI GGYRCC 1 cut(s) 144
BsiEI CGRYCG 2 cut(s) 266, 601
BsiHKAI GWGCWC 1 cut(s) 330
BsiHKCI CYCGRG 1 cut(s) 389
BsiSI CCGG 1 cut(s) 1480
BslFI GGGAC 4 cut(s) 671, 1214, 1412, 1424
BslI CCNNNNNNNGG 3 cut(s) 749, 752, 932
BsmAI GTCTC 2 cut(s) 224, 500
BsmFI GGGAC 4 cut(s) 671, 1214, 1412, 1424
BsmI GAATGC 4 cut(s) 841, 1150, 1351, 1483
BsnI GGCC 3 cut(s) 200, 433, 450
BsoBI CYCGRG 1 cut(s) 389
Bsp1286I GDGCHC 1 cut(s) 330
Bsp143I GATC 5 cut(s) 180, 192, 691, 769, 1338
Bsp19I CCATGG 1 cut(s) 140
BspACI CCGC 4 cut(s) 47, 266, 831, 1392
BspANI GGCC 3 cut(s) 200, 433, 450
BspCNI CTCAG 6 cut(s) 24, 784, 838, 840, 1234, 1432
BspHI TCATGA 2 cut(s) 1111, 1312
BspLI GGNNCC 2 cut(s) 116, 146
BspMAI CTGCAG 1 cut(s) 301
BspPI GGATC 4 cut(s) 187, 188, 686, 764
BspT107I GGYRCC 1 cut(s) 144
BsrDI GCAATG 2 cut(s) 559, 1387
BsrFI RCCGGY 1 cut(s) 1479
BsrI ACTGG 2 cut(s) 514, 926
BssAI RCCGGY 1 cut(s) 1479
BssECI CCNNGG 4 cut(s) 140, 219, 497, 875
BssMI GATC 5 cut(s) 180, 192, 691, 769, 1338
BssT1I CCWWGG 2 cut(s) 140, 497
Bst2UI CCWGG 4 cut(s) 197, 270, 732, 877
Bst4CI ACNGT 2 cut(s) 602, 1225
Bst6I CTCTTC 2 cut(s) 6, 633
BstAPI GCANNNNNTGC 2 cut(s) 296, 305
BstBAI YACGTR 1 cut(s) 749
BstC8I GCNNGC 2 cut(s) 701, 1283
BstDEI CTNAG 6 cut(s) 32, 792, 827, 846, 1242, 1440
BstDSI CCRYGG 1 cut(s) 140
BstEII GGTNACC 1 cut(s) 584
BstF5I GGATG 3 cut(s) 625, 840, 970
BstH2I RGCGCY 2 cut(s) 172, 1618
BstHHI GCGC 4 cut(s) 171, 552, 1275, 1617
BstKTI GATC 5 cut(s) 183, 195, 694, 772, 1341
BstMAI GTCTC 2 cut(s) 224, 500
BstMBI GATC 5 cut(s) 180, 192, 691, 769, 1338
BstMCI CGRYCG 2 cut(s) 266, 601
BstMWI GCNNNNNNNGC 5 cut(s) 287, 296, 305, 416, 680
BstNI CCWGG 4 cut(s) 197, 270, 732, 877
BstNSI RCATGY 1 cut(s) 419
BstPI GGTNACC 1 cut(s) 584
BstSCI CCNGG 4 cut(s) 195, 268, 730, 875
BstSFI CTRYAG 1 cut(s) 297
BstV1I GCAGC 1 cut(s) 283
BstX2I RGATCY 2 cut(s) 192, 691
BstYI RGATCY 2 cut(s) 192, 691
BsuRI GGCC 3 cut(s) 200, 433, 450
BtgI CCRYGG 1 cut(s) 140
BtrI CACGTC 1 cut(s) 1402
BtsCI GGATG 3 cut(s) 625, 840, 970
BtsI GCAGTG 2 cut(s) 405, 1216
BtsIMutI CAGTG 2 cut(s) 405, 1216
Cac8I GCNNGC 2 cut(s) 701, 1283
CciI TCATGA 2 cut(s) 1111, 1312
CfoI GCGC 4 cut(s) 171, 552, 1275, 1617
Cfr10I RCCGGY 1 cut(s) 1479
Cfr13I GGNCC 1 cut(s) 199
CseI GACGC 3 cut(s) 194, 200, 1287
Csp6I GTAC 6 cut(s) 124, 216, 1221, 1549, 1596, 1623
CviAII CATG 5 cut(s) 141, 416, 1095, 1112, 1313
CviQI GTAC 6 cut(s) 124, 216, 1221, 1549, 1596, 1623
DdeI CTNAG 6 cut(s) 32, 792, 827, 846, 1242, 1440
DpnI GATC 5 cut(s) 182, 194, 693, 771, 1340
DpnII GATC 5 cut(s) 180, 192, 691, 769, 1338
DraI TTTAAA 2 cut(s) 708, 760
DraIII CACNNNGTG 1 cut(s) 111
EaeI YGGCCR 1 cut(s) 448
Eam1104I CTCTTC 2 cut(s) 6, 633
EarI CTCTTC 2 cut(s) 6, 633
Eco130I CCWWGG 2 cut(s) 140, 497
Eco147I AGGCCT 1 cut(s) 433
Eco47III AGCGCT 1 cut(s) 1616
Eco57I CTGAAG 3 cut(s) 1254, 1271, 1518
Eco72I CACGTG 1 cut(s) 749
Eco88I CYCGRG 1 cut(s) 389
Eco91I GGTNACC 1 cut(s) 584
EcoO65I GGTNACC 1 cut(s) 584
EcoRII CCWGG 4 cut(s) 195, 268, 730, 875
EcoT14I CCWWGG 2 cut(s) 140, 497
ErhI CCWWGG 2 cut(s) 140, 497
FaeI CATG 5 cut(s) 144, 419, 1098, 1115, 1316
FaqI GGGAC 4 cut(s) 671, 1214, 1412, 1424
FatI CATG 5 cut(s) 140, 415, 1094, 1111, 1312
FauI CCCGC 1 cut(s) 824
FauNDI CATATG 2 cut(s) 1363, 1561
FbaI TGATCA 1 cut(s) 1338
Fnu4HI GCNGC 1 cut(s) 297
FokI GGATG 3 cut(s) 632, 827, 977
Fsp4HI GCNGC 1 cut(s) 297
FspBI CTAG 1 cut(s) 696
FspI TGCGCA 1 cut(s) 551
GlaI GCGC 4 cut(s) 170, 551, 1274, 1616
GluI GCNGC 1 cut(s) 297
GsaI CCCAGC 1 cut(s) 205
GsuI CTGGAG 1 cut(s) 524
HaeII RGCGCY 2 cut(s) 172, 1618
HaeIII GGCC 3 cut(s) 200, 433, 450
HapII CCGG 1 cut(s) 1480
HgaI GACGC 3 cut(s) 194, 200, 1287
HhaI GCGC 4 cut(s) 171, 552, 1275, 1617
Hin1II CATG 5 cut(s) 144, 419, 1098, 1115, 1316
Hin6I GCGC 4 cut(s) 169, 550, 1273, 1615
HinP1I GCGC 4 cut(s) 169, 550, 1273, 1615
HpaII CCGG 1 cut(s) 1480
HphI GGTGA 4 cut(s) 20, 578, 915, 1577
Hpy166II GTNNAC 2 cut(s) 888, 1221
Hpy188III TCNNGA 3 cut(s) 1046, 1112, 1313
Hpy8I GTNNAC 2 cut(s) 888, 1221
Hpy99I CGWCG 1 cut(s) 216
HpyAV CCTTC 5 cut(s) 11, 610, 1191, 1389, 1510
HpyCH4III ACNGT 2 cut(s) 602, 1225
HpyCH4IV ACGT 2 cut(s) 748, 1401
HpyCH4V TGCA 3 cut(s) 290, 299, 320
HpyF10VI GCNNNNNNNGC 5 cut(s) 287, 296, 305, 416, 680
HpyF3I CTNAG 6 cut(s) 32, 792, 827, 846, 1242, 1440
HpySE526I ACGT 2 cut(s) 748, 1401
Hsp92II CATG 5 cut(s) 144, 419, 1098, 1115, 1316
HspAI GCGC 4 cut(s) 169, 550, 1273, 1615
Ksp22I TGATCA 1 cut(s) 1338
Kzo9I GATC 5 cut(s) 180, 192, 691, 769, 1338
LmnI GCTCC 2 cut(s) 114, 499
Lsp1109I GCAGC 1 cut(s) 283
LweI GCATC 3 cut(s) 343, 1129, 1594
MaeI CTAG 1 cut(s) 696
MaeII ACGT 2 cut(s) 748, 1401
MaeIII GTNAC 2 cut(s) 272, 584
MalI GATC 5 cut(s) 182, 194, 693, 771, 1340
MboI GATC 5 cut(s) 180, 192, 691, 769, 1338
MflI RGATCY 2 cut(s) 192, 691
MhlI GDGCHC 1 cut(s) 330
MluCI AATT 6 cut(s) 473, 735, 1049, 1168, 1423, 1489
MlyI GAGTC 3 cut(s) 401, 1086, 1451
MroXI GAANNNNTTC 1 cut(s) 1308
MseI TTAA 4 cut(s) 707, 759, 1418, 1458
MslI CAYNNNNRTG 3 cut(s) 591, 1263, 1362
MspA1I CMGCKG 1 cut(s) 831
MspI CCGG 1 cut(s) 1480
MspR9I CCNGG 4 cut(s) 197, 270, 732, 877
MssI GTTTAAAC 1 cut(s) 760
Mva1269I GAATGC 4 cut(s) 841, 1150, 1351, 1483
MvaI CCWGG 4 cut(s) 197, 270, 732, 877
MwoI GCNNNNNNNGC 5 cut(s) 287, 296, 305, 416, 680
NcoI CCATGG 1 cut(s) 140
NdeI CATATG 2 cut(s) 1363, 1561
NdeII GATC 5 cut(s) 180, 192, 691, 769, 1338
NlaIII CATG 5 cut(s) 144, 419, 1098, 1115, 1316
NlaIV GGNNCC 2 cut(s) 116, 146
NmuCI GTSAC 1 cut(s) 584
NsbI TGCGCA 1 cut(s) 551
NspI RCATGY 1 cut(s) 419
OliI CACNNNNGTG 1 cut(s) 591
PagI TCATGA 2 cut(s) 1111, 1312
PceI AGGCCT 1 cut(s) 433
PctI GAATGC 4 cut(s) 841, 1150, 1351, 1483
PdmI GAANNNNTTC 1 cut(s) 1308
PfeI GAWTC 8 cut(s) 130, 1025, 1042, 1157, 1238, 1304, 1370, 1502
PflMI CCANNNNNTGG 1 cut(s) 932
PkrI GCNGC 1 cut(s) 298
PleI GAGTC 3 cut(s) 400, 1085, 1450
PmaCI CACGTG 1 cut(s) 749
PmeI GTTTAAAC 1 cut(s) 760
PmlI CACGTG 1 cut(s) 749
PpsI GAGTC 3 cut(s) 400, 1085, 1450
Ppu21I YACGTR 1 cut(s) 749
Psp6I CCWGG 4 cut(s) 195, 268, 730, 875
PspCI CACGTG 1 cut(s) 749
PspEI GGTNACC 1 cut(s) 584
PspFI CCCAGC 1 cut(s) 201
PspGI CCWGG 4 cut(s) 195, 268, 730, 875
PspN4I GGNNCC 2 cut(s) 116, 146
PspPI GGNCC 1 cut(s) 199
PstI CTGCAG 1 cut(s) 301
PsuI RGATCY 2 cut(s) 192, 691
RsaI GTAC 6 cut(s) 125, 217, 1222, 1550, 1597, 1624
RsaNI GTAC 6 cut(s) 124, 216, 1221, 1549, 1596, 1623
RseI CAYNNNNRTG 3 cut(s) 591, 1263, 1362
SaqAI TTAA 4 cut(s) 707, 759, 1418, 1458
SatI GCNGC 1 cut(s) 297
Sau3AI GATC 5 cut(s) 180, 192, 691, 769, 1338
Sau96I GGNCC 1 cut(s) 199
ScaI AGTACT 1 cut(s) 1597
SchI GAGTC 3 cut(s) 401, 1086, 1451
ScrFI CCNGG 4 cut(s) 197, 270, 732, 877
SduI GDGCHC 1 cut(s) 330
SfaNI GCATC 3 cut(s) 343, 1129, 1594
SfcI CTRYAG 1 cut(s) 297
SmiMI CAYNNNNRTG 3 cut(s) 591, 1263, 1362
SmlI CTYRAG 2 cut(s) 719, 865
SmoI CTYRAG 2 cut(s) 719, 865
Sse9I AATT 6 cut(s) 473, 735, 1049, 1168, 1423, 1489
SseBI AGGCCT 1 cut(s) 433
SsiI CCGC 4 cut(s) 47, 266, 831, 1392
SspMI CTAG 1 cut(s) 696
StuI AGGCCT 1 cut(s) 433
StyD4I CCNGG 4 cut(s) 195, 268, 730, 875
StyI CCWWGG 2 cut(s) 140, 497
TaaI ACNGT 2 cut(s) 602, 1225
TaiI ACGT 2 cut(s) 751, 1404
TaqI TCGA 1 cut(s) 262
TasI AATT 6 cut(s) 473, 735, 1049, 1168, 1423, 1489
TatI WGTACW 3 cut(s) 1220, 1595, 1622
TfiI GAWTC 8 cut(s) 130, 1025, 1042, 1157, 1238, 1304, 1370, 1502
Tru1I TTAA 4 cut(s) 707, 759, 1418, 1458
Tru9I TTAA 4 cut(s) 707, 759, 1418, 1458
TscAI CASTG 2 cut(s) 412, 1216
TseFI GTSAC 1 cut(s) 584
TseI GCWGC 1 cut(s) 296
Tsp45I GTSAC 1 cut(s) 584
TspGWI ACGGA 1 cut(s) 600
TspRI CASTG 2 cut(s) 412, 1216
Van91I CCANNNNNTGG 1 cut(s) 932
XapI RAATTY 3 cut(s) 473, 1049, 1168
XceI RCATGY 1 cut(s) 419
XmnI GAANNNNTTC 1 cut(s) 1308
XspI CTAG 1 cut(s) 696
ZrmI AGTACT 1 cut(s) 1597
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.