pycom08g00590

Hippocampus abundant transcript 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0009793
Physical Location & Seq
Reverse (-)
493788 .. 497056
3269 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g00590.2

Sequence Viewer

Length: 1143 bp
ATGGTTTGTGAAGGCAGCGCAGATAATGTGGCAGAAGGAAGGCGAGCCTCGACGTTCGGAGTCCTATCGGGGATTAGTTCCTGTGCATTTGTCTGCGGAACCCTCTTCACTCGTTTCCTCAACACTTCCACTACTTTTCAGGTTGCGACGGTGGTGGCAGTTGTGGCAGTTATTTACCTGAGGGTTTTCCTACCGGATTCCAACATAGACAAGAATCTTTCCGCTCCGTTACTCTCAGACGAAAAGATCACAATTAAGGATTCAGCTGAGACTTCAAGTGCTGAGATATTGCAGCCAGCCAAAACATTGCCTTCCTTAAGTGATTTAATCTCCTTGTTGAAAACTAGCACAACGTTTTCGCAAGCTGCAATTGTTGCGTTTTTCAGTAATCTAGCGGATGTTGGCCTCCATGCTTCAATGTTGCAGTACTTTCTAAAGGCGCGGTTTCACTTCAACAAGAATCAGTTTGCTGACCTGATGGTTATTTCCGGGGTTGCAGGCACTGTTTCGCAGCTGGTTCTCATGCCCTTGCTGGCTCCTGCTTTAGGAGAGGAGAGGCTACTTTCGATAGGCCTCCTTTTTAGCTGCGCACAAATGTTTCTTTACGGCATTGCGTGGTCCTTCTGGGTGCCATATGCTGCTGCCATGTTCTCCATCTTTTATGTGTTTGCACATCCATGTATGCGAAGCATTGTATCGAAACAAGTTGGACCGTCTGAGCAGGGGAAGGCTCAAGGGTGCATTTCAGGCATATGTTCACTTGCAAATGTTATATCTCCGATTGCTTTCTCCCCTCTCACAGCTTTGTTCCTGTCCGAAAAGGCGCCATTCAATTTCCCTGGCTTTAGTCTCATTTGCGTTGGATTTGCAGCGATGATAGCCTTCATTCAAAGCGTGATGATAAGGGCTGCTCCTCCCCTCTCGAGCCGACGTGTCAGTGAAAGTTGCAATCACACGGAGCCCTACCCCACCACTCTTGGGGTAAGAAGAAACGACATCCTCGTGGCCAGGTCTATGGTTATGGATGTCACTTTGGCCAGAACATTTGGGGATGGTGGACAAATGGCATCTGATGAAATACCCTGTTGCGGTAGATGTCAATGGGAAGGTAAGTGTACTTCCTGGACGAGAGAGTTTCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1

Protein Analysis

381

Amino Acids

41.0

Weight (kDa)

7.45

Isoelectric Point (pI)

43.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 589
AccB1I GGYRCC 2 cut(s) 628, 823
AccBSI CCGCTC 1 cut(s) 224
AccII CGCG 1 cut(s) 442
AciI CCGC 5 cut(s) 96, 222, 395, 442, 1089
AclI AACGTT 1 cut(s) 353
AcoI YGGCCR 2 cut(s) 1005, 1035
AcyI GRCGYC 1 cut(s) 824
AfaI GTAC 2 cut(s) 428, 1117
AfiI CCNNNNNNNGG 3 cut(s) 545, 978, 1088
AflII CTTAAG 1 cut(s) 316
AflIII ACRYGT 1 cut(s) 931
AgsI TTSAA 6 cut(s) 276, 340, 417, 454, 832, 890
AjiI CACGTC 1 cut(s) 932
AjnI CCWGG 3 cut(s) 838, 1007, 1121
AluBI AGCT 5 cut(s) 266, 365, 514, 585, 803
AluI AGCT 5 cut(s) 266, 365, 514, 585, 803
Alw26I GTCTC 2 cut(s) 263, 854
AlwNI CAGNNNCTG 1 cut(s) 503
Ama87I CYCGRG 1 cut(s) 922
AoxI GGCC 4 cut(s) 403, 571, 1005, 1035
ApeKI GCWGC 9 cut(s) 15, 292, 365, 511, 585, 638, 641, 869, 908
AspLEI GCGC 4 cut(s) 20, 442, 590, 826
AspS9I GGNCC 2 cut(s) 618, 710
AsuC2I CCSGG 1 cut(s) 490
AvaI CYCGRG 1 cut(s) 922
AvaII GGWCC 2 cut(s) 618, 710
AxyI CCTNAGG 1 cut(s) 179
BalI TGGCCA 2 cut(s) 1007, 1037
BanI GGYRCC 2 cut(s) 628, 823
BanII GRGCYC 1 cut(s) 963
BarI GAAGNNNNNNTAC 2 cut(s) 1102, 1134
BauI CACGAG 1 cut(s) 1001
BbvI GCAGC 9 cut(s) 27, 304, 352, 523, 572, 625, 628, 881, 895
BccI CCATC 3 cut(s) 472, 662, 1046
BceAI ACGGC 1 cut(s) 622
BciT130I CCWGG 3 cut(s) 840, 1009, 1123
BcnI CCSGG 1 cut(s) 490
BcoDI GTCTC 2 cut(s) 263, 854
BfaI CTAG 2 cut(s) 345, 392
BfoI RGCGCY 1 cut(s) 827
BfrI CTTAAG 1 cut(s) 316
BisI GCNGC 9 cut(s) 16, 293, 366, 512, 586, 639, 642, 870, 909
BlsI GCNGC 9 cut(s) 17, 294, 367, 513, 587, 640, 643, 871, 910
BmcAI AGTACT 1 cut(s) 428
Bme1390I CCNGG 4 cut(s) 490, 840, 1009, 1123
Bme18I GGWCC 2 cut(s) 618, 710
BmeT110I CYCGRG 1 cut(s) 922
BmgBI CACGTC 1 cut(s) 932
BmgT120I GGNCC 2 cut(s) 618, 710
BmiI GGNNCC 5 cut(s) 100, 537, 630, 825, 960
BmrFI CCNGG 4 cut(s) 490, 840, 1009, 1123
BmsI GCATC 1 cut(s) 1076
BpuEI CTTGAG 1 cut(s) 717
BpuMI CCSGG 1 cut(s) 490
BsaHI GRCGYC 1 cut(s) 824
BsaJI CCNNGG 2 cut(s) 489, 838
BsaWI WCCGGW 1 cut(s) 193
Bsc4I CCNNNNNNNGG 3 cut(s) 545, 978, 1088
Bse21I CCTNAGG 1 cut(s) 179
Bse3DI GCAATG 2 cut(s) 305, 609
BseBI CCWGG 3 cut(s) 840, 1009, 1123
BseDI CCNNGG 2 cut(s) 489, 838
BseGI GGATG 5 cut(s) 403, 673, 996, 1030, 1057
BseLI CCNNNNNNNGG 3 cut(s) 545, 978, 1088
BseMI GCAATG 2 cut(s) 305, 609
BseMII CTCAG 5 cut(s) 170, 249, 258, 273, 708
BseRI GAGGAG 2 cut(s) 566, 903
BseXI GCAGC 9 cut(s) 27, 304, 352, 523, 572, 625, 628, 881, 895
Bsh1236I CGCG 1 cut(s) 442
BshFI GGCC 4 cut(s) 405, 573, 1007, 1037
BshNI GGYRCC 2 cut(s) 628, 823
BsiHKCI CYCGRG 1 cut(s) 922
BsiSI CCGG 2 cut(s) 194, 489
BslI CCNNNNNNNGG 3 cut(s) 545, 978, 1088
BsmAI GTCTC 2 cut(s) 263, 854
BsnI GGCC 4 cut(s) 405, 573, 1007, 1037
BsoBI CYCGRG 1 cut(s) 922
Bsp1286I GDGCHC 1 cut(s) 963
Bsp143I GATC 1 cut(s) 246
BspACI CCGC 5 cut(s) 96, 222, 395, 442, 1089
BspANI GGCC 4 cut(s) 405, 573, 1007, 1037
BspCNI CTCAG 5 cut(s) 171, 248, 259, 274, 709
BspFNI CGCG 1 cut(s) 442
BspLI GGNNCC 5 cut(s) 100, 537, 630, 825, 960
BspT107I GGYRCC 2 cut(s) 628, 823
BspTI CTTAAG 1 cut(s) 316
BsrBI CCGCTC 1 cut(s) 224
BsrDI GCAATG 2 cut(s) 305, 609
BssECI CCNNGG 2 cut(s) 489, 838
BssMI GATC 1 cut(s) 246
BssNI GRCGYC 1 cut(s) 824
BssSI CACGAG 1 cut(s) 1001
Bst2BI CACGAG 1 cut(s) 1001
Bst2UI CCWGG 3 cut(s) 840, 1009, 1123
Bst4CI ACNGT 3 cut(s) 151, 505, 714
Bst6I CTCTTC 1 cut(s) 110
BstACI GRCGYC 1 cut(s) 824
BstAFI CTTAAG 1 cut(s) 316
BstAPI GCANNNNNTGC 1 cut(s) 374
BstC8I GCNNGC 5 cut(s) 45, 297, 363, 499, 534
BstDEI CTNAG 5 cut(s) 179, 235, 267, 282, 717
BstENI CCTNNNNNAGG 1 cut(s) 543
BstF5I GGATG 5 cut(s) 403, 673, 996, 1030, 1057
BstFNI CGCG 1 cut(s) 442
BstH2I RGCGCY 1 cut(s) 827
BstHHI GCGC 4 cut(s) 20, 442, 590, 826
BstKTI GATC 1 cut(s) 249
BstMAI GTCTC 2 cut(s) 263, 854
BstMBI GATC 1 cut(s) 246
BstMWI GCNNNNNNNGC 4 cut(s) 164, 374, 747, 878
BstNI CCWGG 3 cut(s) 840, 1009, 1123
BstSCI CCNGG 4 cut(s) 488, 838, 1007, 1121
BstUI CGCG 1 cut(s) 442
BstV1I GCAGC 9 cut(s) 27, 304, 352, 523, 572, 625, 628, 881, 895
BstXI CCANNNNNNTGG 1 cut(s) 1015
Bsu36I CCTNAGG 1 cut(s) 179
BsuRI GGCC 4 cut(s) 405, 573, 1007, 1037
BtgZI GCGATG 1 cut(s) 887
BtrI CACGTC 1 cut(s) 932
BtsCI GGATG 5 cut(s) 403, 673, 996, 1030, 1057
BtsIMutI CAGTG 2 cut(s) 501, 943
Cac8I GCNNGC 5 cut(s) 45, 297, 363, 499, 534
CaiI CAGNNNCTG 1 cut(s) 503
CfoI GCGC 4 cut(s) 20, 442, 590, 826
Cfr13I GGNCC 2 cut(s) 618, 710
Csp6I GTAC 2 cut(s) 427, 1116
CspCI CAANNNNNGTGG 2 cut(s) 958, 993
CviAII CATG 4 cut(s) 410, 523, 646, 678
CviQI GTAC 2 cut(s) 427, 1116
DdeI CTNAG 5 cut(s) 179, 235, 267, 282, 717
DinI GGCGCC 1 cut(s) 825
DpnI GATC 1 cut(s) 248
DpnII GATC 1 cut(s) 246
EaeI YGGCCR 2 cut(s) 1005, 1035
Eam1104I CTCTTC 1 cut(s) 110
EarI CTCTTC 1 cut(s) 110
Eco147I AGGCCT 1 cut(s) 573
Eco24I GRGCYC 1 cut(s) 963
Eco47I GGWCC 2 cut(s) 618, 710
Eco81I CCTNAGG 1 cut(s) 179
Eco88I CYCGRG 1 cut(s) 922
EcoNI CCTNNNNNAGG 1 cut(s) 543
EcoRII CCWGG 3 cut(s) 838, 1007, 1121
EcoT38I GRGCYC 1 cut(s) 963
EgeI GGCGCC 1 cut(s) 825
EheI GGCGCC 1 cut(s) 825
FaeI CATG 4 cut(s) 413, 526, 649, 681
FatI CATG 4 cut(s) 409, 522, 645, 677
FauNDI CATATG 2 cut(s) 634, 752
Fnu4HI GCNGC 9 cut(s) 16, 293, 366, 512, 586, 639, 642, 870, 909
FokI GGATG 5 cut(s) 410, 660, 983, 1037, 1064
FriOI GRGCYC 1 cut(s) 963
Fsp4HI GCNGC 9 cut(s) 16, 293, 366, 512, 586, 639, 642, 870, 909
FspBI CTAG 2 cut(s) 345, 392
FspI TGCGCA 1 cut(s) 589
GlaI GCGC 4 cut(s) 19, 441, 589, 825
GluI GCNGC 9 cut(s) 16, 293, 366, 512, 586, 639, 642, 870, 909
HaeII RGCGCY 1 cut(s) 827
HaeIII GGCC 4 cut(s) 405, 573, 1007, 1037
HapII CCGG 2 cut(s) 194, 489
HhaI GCGC 4 cut(s) 20, 442, 590, 826
Hin1I GRCGYC 1 cut(s) 824
Hin1II CATG 4 cut(s) 413, 526, 649, 681
Hin6I GCGC 4 cut(s) 18, 440, 588, 824
HinP1I GCGC 4 cut(s) 18, 440, 588, 824
HinfI GANTC 5 cut(s) 60, 197, 214, 260, 460
HpaII CCGG 2 cut(s) 194, 489
Hpy166II GTNNAC 3 cut(s) 758, 1058, 1116
Hpy188I TCNGA 6 cut(s) 59, 238, 718, 780, 817, 1072
Hpy188III TCNNGA 1 cut(s) 922
Hpy8I GTNNAC 3 cut(s) 758, 1058, 1116
Hpy99I CGWCG 3 cut(s) 55, 151, 933
HpyAV CCTTC 8 cut(s) 5, 29, 33, 321, 631, 721, 892, 1100
HpyCH4III ACNGT 3 cut(s) 151, 505, 714
HpyCH4IV ACGT 3 cut(s) 53, 353, 931
HpyF10VI GCNNNNNNNGC 4 cut(s) 164, 374, 747, 878
HpyF3I CTNAG 5 cut(s) 179, 235, 267, 282, 717
HpySE526I ACGT 3 cut(s) 53, 353, 931
Hsp92I GRCGYC 1 cut(s) 824
Hsp92II CATG 4 cut(s) 413, 526, 649, 681
HspAI GCGC 4 cut(s) 18, 440, 588, 824
KasI GGCGCC 1 cut(s) 823
Kzo9I GATC 1 cut(s) 246
LmnI GCTCC 4 cut(s) 229, 541, 916, 958
Lsp1109I GCAGC 9 cut(s) 27, 304, 352, 523, 572, 625, 628, 881, 895
LweI GCATC 1 cut(s) 1076
MaeI CTAG 2 cut(s) 345, 392
MaeII ACGT 3 cut(s) 53, 353, 931
MaeIII GTNAC 2 cut(s) 228, 1027
MalI GATC 1 cut(s) 248
MbiI CCGCTC 1 cut(s) 224
MboI GATC 1 cut(s) 246
MboII GAAGA 2 cut(s) 97, 999
MfeI CAATTG 1 cut(s) 369
MhlI GDGCHC 1 cut(s) 963
MlsI TGGCCA 2 cut(s) 1007, 1037
MluCI AATT 3 cut(s) 252, 369, 832
MluNI TGGCCA 2 cut(s) 1007, 1037
Mly113I GGCGCC 1 cut(s) 824
MlyI GAGTC 1 cut(s) 69
MmeI TCCRAC 3 cut(s) 225, 688, 841
Mox20I TGGCCA 2 cut(s) 1007, 1037
MscI TGGCCA 2 cut(s) 1007, 1037
MseI TTAA 3 cut(s) 255, 317, 326
MslI CAYNNNNRTG 2 cut(s) 676, 1001
Msp20I TGGCCA 2 cut(s) 1007, 1037
MspA1I CMGCKG 2 cut(s) 266, 514
MspCI CTTAAG 1 cut(s) 316
MspI CCGG 2 cut(s) 194, 489
MspR9I CCNGG 4 cut(s) 490, 840, 1009, 1123
MunI CAATTG 1 cut(s) 369
MvaI CCWGG 3 cut(s) 840, 1009, 1123
MvnI CGCG 1 cut(s) 442
MwoI GCNNNNNNNGC 4 cut(s) 164, 374, 747, 878
NarI GGCGCC 1 cut(s) 824
NciI CCSGG 1 cut(s) 490
NdeI CATATG 2 cut(s) 634, 752
NdeII GATC 1 cut(s) 246
NlaIII CATG 4 cut(s) 413, 526, 649, 681
NlaIV GGNNCC 5 cut(s) 100, 537, 630, 825, 960
NmuCI GTSAC 1 cut(s) 1027
NsbI TGCGCA 1 cut(s) 589
PaeR7I CTCGAG 1 cut(s) 922
PceI AGGCCT 1 cut(s) 573
PcsI WCGNNNNNNNCGW 1 cut(s) 999
PfeI GAWTC 4 cut(s) 197, 214, 260, 460
PfoI TCCNGGA 1 cut(s) 1121
PkrI GCNGC 9 cut(s) 17, 294, 367, 513, 587, 640, 643, 871, 910
PleI GAGTC 1 cut(s) 68
PluTI GGCGCC 1 cut(s) 827
PpsI GAGTC 1 cut(s) 68
Psp1406I AACGTT 1 cut(s) 353
Psp6I CCWGG 3 cut(s) 838, 1007, 1121
PspGI CCWGG 3 cut(s) 838, 1007, 1121
PspN4I GGNNCC 5 cut(s) 100, 537, 630, 825, 960
PspPI GGNCC 2 cut(s) 618, 710
PstNI CAGNNNCTG 1 cut(s) 503
PvuII CAGCTG 2 cut(s) 266, 514
RsaI GTAC 2 cut(s) 428, 1117
RsaNI GTAC 2 cut(s) 427, 1116
RseI CAYNNNNRTG 2 cut(s) 676, 1001
SaqAI TTAA 3 cut(s) 255, 317, 326
SatI GCNGC 9 cut(s) 16, 293, 366, 512, 586, 639, 642, 870, 909
Sau3AI GATC 1 cut(s) 246
Sau96I GGNCC 2 cut(s) 618, 710
ScaI AGTACT 1 cut(s) 428
SchI GAGTC 1 cut(s) 69
ScrFI CCNGG 4 cut(s) 490, 840, 1009, 1123
SduI GDGCHC 1 cut(s) 963
SfaNI GCATC 1 cut(s) 1076
SfoI GGCGCC 1 cut(s) 825
Sfr274I CTCGAG 1 cut(s) 922
SinI GGWCC 2 cut(s) 618, 710
SlaI CTCGAG 1 cut(s) 922
SmiMI CAYNNNNRTG 2 cut(s) 676, 1001
SmlI CTYRAG 3 cut(s) 316, 732, 922
SmoI CTYRAG 3 cut(s) 316, 732, 922
Sse9I AATT 3 cut(s) 252, 369, 832
SseBI AGGCCT 1 cut(s) 573
SsiI CCGC 5 cut(s) 96, 222, 395, 442, 1089
SspDI GGCGCC 1 cut(s) 823
SspMI CTAG 2 cut(s) 345, 392
StuI AGGCCT 1 cut(s) 573
StyD4I CCNGG 4 cut(s) 488, 838, 1007, 1121
TaaI ACNGT 3 cut(s) 151, 505, 714
TaiI ACGT 3 cut(s) 56, 356, 934
TaqI TCGA 4 cut(s) 50, 566, 698, 923
TasI AATT 3 cut(s) 252, 369, 832
TatI WGTACW 2 cut(s) 426, 1115
TfiI GAWTC 4 cut(s) 197, 214, 260, 460
Tru1I TTAA 3 cut(s) 255, 317, 326
Tru9I TTAA 3 cut(s) 255, 317, 326
TscAI CASTG 2 cut(s) 508, 943
TseFI GTSAC 1 cut(s) 1027
TseI GCWGC 9 cut(s) 15, 292, 365, 511, 585, 638, 641, 869, 908
Tsp45I GTSAC 1 cut(s) 1027
TspDTI ATGAA 2 cut(s) 874, 1089
TspGWI ACGGA 2 cut(s) 216, 971
TspRI CASTG 2 cut(s) 508, 943
Vha464I CTTAAG 1 cut(s) 316
VpaK11BI GGWCC 2 cut(s) 618, 710
XagI CCTNNNNNAGG 1 cut(s) 543
XhoI CTCGAG 1 cut(s) 922
XspI CTAG 2 cut(s) 345, 392
ZrmI AGTACT 1 cut(s) 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.