pycom08g01940

Transmembrane protein 136

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0007728
Physical Location & Seq
Reverse (-)
1425408 .. 1427598
2191 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 714 bp
ATGGAGGAGCAAATTGCAAGTGTGGTGGTGCTGGGGGTGATTTCATGGACTACGGCGTTTCTGTTCATACGAAAGATGCTTCCGAAGCGATCGTTCGACTTCTGCAACCGCCTTGTATCCACAGTTCATGCAACCGTGGCTGTGACCTTAGCTTCCCTCTCTGTTCAAGATTGGAACTGTCCTGTGTGCCCTCTGGCTTCAACTTCTTCTCCTCGTCAGTTGAAAGCACAGTCTGTGAGCCTTTCTTATCTCATATACGATCTCGTGTGTTGCCTCTTTGACAAGAAATTCAATCCTGATAATTTCTTCCACCATTTGGTCAGCATTGTTGGCATTGGAGCAGGCCTTTCCTACCAAAAGTGTGGGTCGGAGATGGTGGCAGCATTGTGGATAACAGAGTTGTCCAGCCCCTTCCTCCATACAAGGGAGCTTCTCAAAGAGCTTGGTTACAGAGACACCGATCTATACCTAGCAGCCGATGTAATTATATTCACGGTTGCAAGGATGGTAGGCGGGCCTTATCTCACCTACGTGACTCTGTCTGCTAACAACCCATTCATCATCAAGGCGATGGCGGCAGGGTTGCAGCTGGTGAGCGCTTTCTGGTTCTACAAGATTGCGAGGATGGTCAAGTACAAATTGACCAAGAGGACGACCACTGCTGCTGCATCTAGGAAACAAGTCGATCCCCCCCCAACCCATAAACACACGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TRAM_LAG1_CLN8

Protein Analysis

238

Amino Acids

26.42

Weight (kDa)

9.32

Isoelectric Point (pI)

35.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRAM_LAG1_CLN8 PF03798 33 - 208 1.4e-23 TLC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 316
AciI CCGC 3 cut(s) 109, 513, 575
AclWI GGATC 1 cut(s) 680
AcsI RAATTY 1 cut(s) 287
AfaI GTAC 1 cut(s) 635
AfeI AGCGCT 1 cut(s) 598
AfiI CCNNNNNNNGG 2 cut(s) 316, 424
AflIII ACRYGT 1 cut(s) 708
AgsI TTSAA 4 cut(s) 167, 201, 223, 292
AleI CACNNNNGTG 1 cut(s) 530
AluBI AGCT 4 cut(s) 152, 430, 442, 589
AluI AGCT 4 cut(s) 152, 430, 442, 589
Alw26I GTCTC 1 cut(s) 447
AlwI GGATC 1 cut(s) 680
Aor51HI AGCGCT 1 cut(s) 598
AoxI GGCC 2 cut(s) 343, 515
ApeKI GCWGC 5 cut(s) 380, 473, 586, 662, 665
ApoI RAATTY 1 cut(s) 287
AspLEI GCGC 1 cut(s) 599
AspS9I GGNCC 1 cut(s) 515
AsuHPI GGTGA 3 cut(s) 49, 517, 604
BaeGI GKGCMC 1 cut(s) 191
BauI CACGAG 1 cut(s) 263
BbvI GCAGC 5 cut(s) 392, 485, 598, 649, 652
BccI CCATC 4 cut(s) 367, 499, 565, 619
BceAI ACGGC 1 cut(s) 69
BciVI GTATCC 1 cut(s) 127
BcoDI GTCTC 1 cut(s) 447
BfaI CTAG 2 cut(s) 470, 672
BfoI RGCGCY 1 cut(s) 600
BfuI GTATCC 1 cut(s) 127
BisI GCNGC 6 cut(s) 381, 474, 576, 587, 663, 666
BlsI GCNGC 6 cut(s) 382, 475, 577, 588, 664, 667
BmgT120I GGNCC 1 cut(s) 515
BmsI GCATC 2 cut(s) 66, 677
Bpu10I CCTNAGC 1 cut(s) 148
BsaAI YACGTR 2 cut(s) 532, 711
BsaJI CCNNGG 1 cut(s) 135
BsaXI ACNNNNNCTCC 2 cut(s) 193, 223
Bsc4I CCNNNNNNNGG 2 cut(s) 316, 424
BseDI CCNNGG 1 cut(s) 135
BseGI GGATG 2 cut(s) 510, 630
BseLI CCNNNNNNNGG 2 cut(s) 316, 424
BseRI GAGGAG 2 cut(s) 20, 201
BseSI GKGCMC 1 cut(s) 191
BseXI GCAGC 5 cut(s) 392, 485, 598, 649, 652
BseYI CCCAGC 1 cut(s) 31
Bsh1285I CGRYCG 1 cut(s) 92
BshFI GGCC 2 cut(s) 345, 517
BsiEI CGRYCG 1 cut(s) 92
BslI CCNNNNNNNGG 2 cut(s) 316, 424
BsmAI GTCTC 1 cut(s) 447
BsnI GGCC 2 cut(s) 345, 517
Bsp1286I GDGCHC 1 cut(s) 191
Bsp143I GATC 4 cut(s) 89, 259, 460, 685
BspACI CCGC 3 cut(s) 109, 513, 575
BspANI GGCC 2 cut(s) 345, 517
BspPI GGATC 1 cut(s) 680
BssECI CCNNGG 1 cut(s) 135
BssMI GATC 4 cut(s) 89, 259, 460, 685
BssSI CACGAG 1 cut(s) 263
Bst2BI CACGAG 1 cut(s) 263
Bst4CI ACNGT 5 cut(s) 124, 136, 179, 231, 496
BstBAI YACGTR 2 cut(s) 532, 711
BstC8I GCNNGC 2 cut(s) 343, 515
BstDEI CTNAG 1 cut(s) 148
BstDSI CCRYGG 1 cut(s) 135
BstF5I GGATG 2 cut(s) 510, 630
BstH2I RGCGCY 1 cut(s) 600
BstHHI GCGC 1 cut(s) 599
BstKTI GATC 4 cut(s) 92, 262, 463, 688
BstMAI GTCTC 1 cut(s) 447
BstMBI GATC 4 cut(s) 89, 259, 460, 685
BstMCI CGRYCG 1 cut(s) 92
BstMWI GCNNNNNNNGC 4 cut(s) 85, 137, 330, 575
BstSLI GKGCMC 1 cut(s) 191
BstV1I GCAGC 5 cut(s) 392, 485, 598, 649, 652
BstXI CCANNNNNNTGG 1 cut(s) 362
BsuI GTATCC 1 cut(s) 127
BsuRI GGCC 2 cut(s) 345, 517
BtgI CCRYGG 1 cut(s) 135
BtgZI GCGATG 1 cut(s) 584
BtsCI GGATG 2 cut(s) 510, 630
BtsI GCAGTG 1 cut(s) 657
BtsIMutI CAGTG 1 cut(s) 657
Cac8I GCNNGC 2 cut(s) 343, 515
CfoI GCGC 1 cut(s) 599
Cfr13I GGNCC 1 cut(s) 515
Csp6I GTAC 1 cut(s) 634
CspCI CAANNNNNGTGG 2 cut(s) 6, 41
CviAII CATG 2 cut(s) 45, 128
CviQI GTAC 1 cut(s) 634
DdeI CTNAG 1 cut(s) 148
DpnI GATC 4 cut(s) 91, 261, 462, 687
DpnII GATC 4 cut(s) 89, 259, 460, 685
Eco147I AGGCCT 1 cut(s) 345
Eco47III AGCGCT 1 cut(s) 598
FaeI CATG 2 cut(s) 48, 131
FaiI YATR 9 cut(s) 46, 68, 129, 254, 256, 420, 466, 488, 702
FatI CATG 2 cut(s) 44, 127
FauI CCCGC 1 cut(s) 506
Fnu4HI GCNGC 6 cut(s) 381, 474, 576, 587, 663, 666
FokI GGATG 2 cut(s) 517, 637
Fsp4HI GCNGC 6 cut(s) 381, 474, 576, 587, 663, 666
FspBI CTAG 2 cut(s) 470, 672
GlaI GCGC 1 cut(s) 598
GluI GCNGC 6 cut(s) 381, 474, 576, 587, 663, 666
GsaI CCCAGC 1 cut(s) 35
HaeII RGCGCY 1 cut(s) 600
HaeIII GGCC 2 cut(s) 345, 517
HhaI GCGC 1 cut(s) 599
Hin1II CATG 2 cut(s) 48, 131
Hin6I GCGC 1 cut(s) 597
HinP1I GCGC 1 cut(s) 597
HinfI GANTC 1 cut(s) 535
HphI GGTGA 3 cut(s) 49, 517, 604
Hpy188I TCNGA 2 cut(s) 84, 370
Hpy188III TCNNGA 2 cut(s) 167, 296
HpyAV CCTTC 1 cut(s) 421
HpyCH4III ACNGT 5 cut(s) 124, 136, 179, 231, 496
HpyCH4IV ACGT 2 cut(s) 531, 710
HpyCH4V TGCA 6 cut(s) 17, 105, 131, 500, 586, 668
HpyF10VI GCNNNNNNNGC 4 cut(s) 85, 137, 330, 575
HpyF3I CTNAG 1 cut(s) 148
HpySE526I ACGT 2 cut(s) 531, 710
Hsp92II CATG 2 cut(s) 48, 131
HspAI GCGC 1 cut(s) 597
Kzo9I GATC 4 cut(s) 89, 259, 460, 685
LmnI GCTCC 3 cut(s) 7, 338, 427
LpnPI CCDG 9 cut(s) 17, 179, 195, 309, 327, 418, 564, 575, 589
Lsp1109I GCAGC 5 cut(s) 392, 485, 598, 649, 652
LweI GCATC 2 cut(s) 66, 677
MaeI CTAG 2 cut(s) 470, 672
MaeII ACGT 2 cut(s) 531, 710
MaeIII GTNAC 3 cut(s) 142, 446, 532
MalI GATC 4 cut(s) 91, 261, 462, 687
MboI GATC 4 cut(s) 89, 259, 460, 685
MboII GAAGA 2 cut(s) 198, 298
MhlI GDGCHC 1 cut(s) 191
MluCI AATT 5 cut(s) 12, 287, 301, 483, 638
MlyI GAGTC 1 cut(s) 529
MmeI TCCRAC 1 cut(s) 348
MnlI CCTC 7 cut(s) 167, 201, 222, 284, 425, 615, 642
MslI CAYNNNNRTG 1 cut(s) 530
MspA1I CMGCKG 1 cut(s) 589
MwoI GCNNNNNNNGC 4 cut(s) 85, 137, 330, 575
NdeII GATC 4 cut(s) 89, 259, 460, 685
NlaIII CATG 2 cut(s) 48, 131
NmuCI GTSAC 2 cut(s) 142, 532
OliI CACNNNNGTG 1 cut(s) 530
PceI AGGCCT 1 cut(s) 345
PflFI GACNNNGTC 1 cut(s) 538
PflMI CCANNNNNTGG 1 cut(s) 316
PkrI GCNGC 6 cut(s) 382, 475, 577, 588, 664, 667
Ple19I CGATCG 1 cut(s) 92
PleI GAGTC 1 cut(s) 529
PpsI GAGTC 1 cut(s) 529
Ppu21I YACGTR 2 cut(s) 532, 711
PspFI CCCAGC 1 cut(s) 31
PspPI GGNCC 1 cut(s) 515
PsrI GAACNNNNNNTAC 2 cut(s) 108, 140
PsyI GACNNNGTC 1 cut(s) 538
PvuI CGATCG 1 cut(s) 92
PvuII CAGCTG 1 cut(s) 589
RsaI GTAC 1 cut(s) 635
RsaNI GTAC 1 cut(s) 634
RseI CAYNNNNRTG 1 cut(s) 530
SatI GCNGC 6 cut(s) 381, 474, 576, 587, 663, 666
Sau3AI GATC 4 cut(s) 89, 259, 460, 685
Sau96I GGNCC 1 cut(s) 515
SchI GAGTC 1 cut(s) 529
SduI GDGCHC 1 cut(s) 191
SetI ASST 9 cut(s) 149, 154, 432, 444, 471, 530, 534, 591, 713
SfaNI GCATC 2 cut(s) 66, 677
SmiMI CAYNNNNRTG 1 cut(s) 530
Sse9I AATT 5 cut(s) 12, 287, 301, 483, 638
SseBI AGGCCT 1 cut(s) 345
SsiI CCGC 3 cut(s) 109, 513, 575
SspMI CTAG 2 cut(s) 470, 672
StuI AGGCCT 1 cut(s) 345
TaaI ACNGT 5 cut(s) 124, 136, 179, 231, 496
TaiI ACGT 2 cut(s) 534, 713
TaqI TCGA 2 cut(s) 96, 684
TasI AATT 5 cut(s) 12, 287, 301, 483, 638
TatI WGTACW 1 cut(s) 633
TauI GCSGC 1 cut(s) 578
TscAI CASTG 1 cut(s) 664
TseFI GTSAC 2 cut(s) 142, 532
TseI GCWGC 5 cut(s) 380, 473, 586, 662, 665
Tsp45I GTSAC 2 cut(s) 142, 532
TspDTI ATGAA 4 cut(s) 33, 55, 116, 547
TspRI CASTG 1 cut(s) 664
Tth111I GACNNNGTC 1 cut(s) 538
Van91I CCANNNNNTGG 1 cut(s) 316
XapI RAATTY 1 cut(s) 287
XspI CTAG 2 cut(s) 470, 672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.