pycom08g04740
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0011531
Physical Location & Seq
Reverse (-)
3574847 .. 3575935
1089 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 864 bp
ATGGCTGATGATCGTGACCAGGTTGTAGACAGTGTTCAAGAAGACTTGCAACGTTTGAGGAAAGAGAATGAAACCCTAAGAGTCCTGCTTCAACACTTGACCACTAAGCGCTCAACACTTGAGCAGCAGCAGCTGCGCCAAATGCATATCCAACAACAACCGTGTGATCATCGATATATCATGACCACGAATATCAACAGCGCGATTACGAGCAAGAGACAGAGAACTGATGAGCATCCCGATCAGTTTCCGGCATACCACAATAAGATATCACAGTTCCTAGTGAAATCCGATTCTAAAGACAACAGCCTTGTAAGTCAAGTCCTCCCTTATAATATTGTGAAGGATGGATATCAATGGAGGAAATATGGACAGAAGGTCACCAAAGACAACCCTTCACCCCCTCGAGCTTATTTCAGGTGTTCTTTCGCTCCTCGATGCCCTGTCAAAAAGAAGGTACAGAGGTGCATAGAGGATGATTCCATCCTCGTGGCGACATACAAAGGAGAGCACAACCACGAAGCCATCAATGATTCTGTACCATTTGGACAATTTTCATGTTCACATAAGAACATAATTGTGCCCGTTAATTTTCCTTCAAGTGGTATCAAAACTAATAATGACGTGATTAATATAAGTCCCTCTCTGCCGGAGCCTGTAACGCTCGATCTTAGTCTCTCGGGATCCAGTAATCGAGAAAATGGAGGATTGGGAAGCCCTCAAAGCATGGTTAGCCCCGGAAGAGTTGAGGATTACGTTGCTTCGTTAACCAAGGATGCTAATTTCACACAAACTTTAGTTGCTGCAGTAATAGACCAAATTCATAGATTGGAGCGGATAACTCCTTATAGTCAAGATACGTAG

Protein Analysis

288

Amino Acids

32.59

Weight (kDa)

8.21

Isoelectric Point (pI)

60.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 115 - 174 4.3e-23 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 333
AccBSI CCGCTC 1 cut(s) 835
AccI GTMKAC 1 cut(s) 27
AccII CGCG 1 cut(s) 203
AciI CCGC 1 cut(s) 835
AclI AACGTT 1 cut(s) 52
AclWI GGATC 2 cut(s) 678, 691
AcsI RAATTY 1 cut(s) 819
AfaI GTAC 2 cut(s) 459, 540
AfeI AGCGCT 1 cut(s) 110
AfiI CCNNNNNNNGG 1 cut(s) 602
AgsI TTSAA 3 cut(s) 38, 92, 600
AhdI GACNNNNNGTC 1 cut(s) 377
AjiI CACGTC 1 cut(s) 625
AjnI CCWGG 1 cut(s) 18
AluBI AGCT 2 cut(s) 133, 410
AluI AGCT 2 cut(s) 133, 410
Alw21I GWGCWC 1 cut(s) 513
Alw26I GTCTC 2 cut(s) 211, 680
AlwI GGATC 2 cut(s) 678, 691
AlwNI CAGNNNCTG 1 cut(s) 133
Ama87I CYCGRG 2 cut(s) 405, 679
Aor51HI AGCGCT 1 cut(s) 110
ApeKI GCWGC 5 cut(s) 124, 127, 130, 133, 803
ApoI RAATTY 1 cut(s) 819
AseI ATTAAT 1 cut(s) 630
AspLEI GCGC 3 cut(s) 111, 138, 203
AsuC2I CCSGG 1 cut(s) 738
AsuHPI GGTGA 2 cut(s) 373, 390
AvaI CYCGRG 2 cut(s) 405, 679
BaeGI GKGCMC 1 cut(s) 585
BaeI ACNNNNGTAYC 2 cut(s) 449, 482
BamHI GGATCC 1 cut(s) 683
BauI CACGAG 1 cut(s) 488
BbsI GAAGAC 1 cut(s) 48
Bbv12I GWGCWC 1 cut(s) 513
BbvI GCAGC 5 cut(s) 120, 136, 139, 142, 790
BccI CCATC 3 cut(s) 341, 491, 533
BciT130I CCWGG 1 cut(s) 20
BclI TGATCA 1 cut(s) 166
BcnI CCSGG 1 cut(s) 738
BcoDI GTCTC 2 cut(s) 211, 680
BfaI CTAG 1 cut(s) 281
BfmI CTRYAG 1 cut(s) 804
BfoI RGCGCY 1 cut(s) 112
BisI GCNGC 5 cut(s) 125, 128, 131, 134, 804
BlsI GCNGC 5 cut(s) 126, 129, 132, 135, 805
Bme1390I CCNGG 2 cut(s) 20, 738
BmeRI GACNNNNNGTC 1 cut(s) 377
BmeT110I CYCGRG 2 cut(s) 405, 679
BmgBI CACGTC 1 cut(s) 625
BmiI GGNNCC 2 cut(s) 654, 685
BmrFI CCNGG 2 cut(s) 20, 738
BmsI GCATC 3 cut(s) 244, 428, 766
BpiI GAAGAC 1 cut(s) 48
BpuEI CTTGAG 1 cut(s) 140
BpuMI CCSGG 1 cut(s) 738
Bsa29I ATCGAT 1 cut(s) 172
BsaAI YACGTR 1 cut(s) 861
BsaBI GATNNNNATC 2 cut(s) 234, 351
BsaJI CCNNGG 2 cut(s) 736, 771
Bsc4I CCNNNNNNNGG 1 cut(s) 602
Bse1I ACTGG 1 cut(s) 687
Bse8I GATNNNNATC 2 cut(s) 234, 351
BseBI CCWGG 1 cut(s) 20
BseCI ATCGAT 1 cut(s) 172
BseDI CCNNGG 2 cut(s) 736, 771
BseGI GGATG 5 cut(s) 235, 352, 481, 483, 781
BseJI GATNNNNATC 2 cut(s) 234, 351
BseLI CCNNNNNNNGG 1 cut(s) 602
BseNI ACTGG 1 cut(s) 687
BseRI GAGGAG 1 cut(s) 423
BseSI GKGCMC 1 cut(s) 585
BseXI GCAGC 5 cut(s) 120, 136, 139, 142, 790
Bsh1236I CGCG 1 cut(s) 203
BshVI ATCGAT 1 cut(s) 172
BsiHKAI GWGCWC 1 cut(s) 513
BsiHKCI CYCGRG 2 cut(s) 405, 679
BsiSI CCGG 3 cut(s) 251, 650, 738
BslFI GGGAC 1 cut(s) 624
BslI CCNNNNNNNGG 1 cut(s) 602
BsmAI GTCTC 2 cut(s) 211, 680
BsmFI GGGAC 1 cut(s) 624
BsoBI CYCGRG 2 cut(s) 405, 679
Bsp1286I GDGCHC 2 cut(s) 513, 585
Bsp143I GATC 5 cut(s) 10, 166, 241, 667, 683
BspACI CCGC 1 cut(s) 835
BspDI ATCGAT 1 cut(s) 172
BspFNI CGCG 1 cut(s) 203
BspHI TCATGA 1 cut(s) 180
BspLI GGNNCC 2 cut(s) 654, 685
BspMAI CTGCAG 1 cut(s) 808
BspPI GGATC 2 cut(s) 678, 691
BsrBI CCGCTC 1 cut(s) 835
BsrI ACTGG 1 cut(s) 687
BssECI CCNNGG 2 cut(s) 736, 771
BssMI GATC 5 cut(s) 10, 166, 241, 667, 683
BssSI CACGAG 1 cut(s) 488
BssT1I CCWWGG 1 cut(s) 771
Bst2BI CACGAG 1 cut(s) 488
Bst2UI CCWGG 1 cut(s) 20
Bst4CI ACNGT 3 cut(s) 32, 162, 276
Bst6I CTCTTC 1 cut(s) 736
BstAPI GCANNNNNTGC 1 cut(s) 133
BstBAI YACGTR 1 cut(s) 861
BstDEI CTNAG 3 cut(s) 77, 105, 671
BstEII GGTNACC 1 cut(s) 379
BstF5I GGATG 5 cut(s) 235, 352, 481, 483, 781
BstFNI CGCG 1 cut(s) 203
BstH2I RGCGCY 1 cut(s) 112
BstHHI GCGC 3 cut(s) 111, 138, 203
BstKTI GATC 5 cut(s) 13, 169, 244, 670, 686
BstMAI GTCTC 2 cut(s) 211, 680
BstMBI GATC 5 cut(s) 10, 166, 241, 667, 683
BstMWI GCNNNNNNNGC 6 cut(s) 130, 133, 142, 661, 723, 732
BstNI CCWGG 1 cut(s) 20
BstPI GGTNACC 1 cut(s) 379
BstSCI CCNGG 2 cut(s) 18, 736
BstSFI CTRYAG 1 cut(s) 804
BstSLI GKGCMC 1 cut(s) 585
BstSNI TACGTA 1 cut(s) 861
BstUI CGCG 1 cut(s) 203
BstV1I GCAGC 5 cut(s) 120, 136, 139, 142, 790
BstV2I GAAGAC 1 cut(s) 48
BstX2I RGATCY 1 cut(s) 683
BstXI CCANNNNNNTGG 1 cut(s) 490
BstYI RGATCY 1 cut(s) 683
Bsu15I ATCGAT 1 cut(s) 172
BsuTUI ATCGAT 1 cut(s) 172
BtrI CACGTC 1 cut(s) 625
BtsCI GGATG 5 cut(s) 235, 352, 481, 483, 781
BtsIMutI CAGTG 1 cut(s) 37
CaiI CAGNNNCTG 1 cut(s) 133
CciI TCATGA 1 cut(s) 180
CfoI GCGC 3 cut(s) 111, 138, 203
ClaI ATCGAT 1 cut(s) 172
CsiI ACCWGGT 1 cut(s) 18
Csp6I GTAC 2 cut(s) 458, 539
CviAII CATG 3 cut(s) 181, 558, 727
CviJI RGCY 8 cut(s) 5, 133, 309, 410, 524, 655, 717, 735
CviKI_1 RGCY 8 cut(s) 5, 133, 309, 410, 524, 655, 717, 735
CviQI GTAC 2 cut(s) 458, 539
DdeI CTNAG 3 cut(s) 77, 105, 671
DpnI GATC 5 cut(s) 12, 168, 243, 669, 685
DpnII GATC 5 cut(s) 10, 166, 241, 667, 683
DriI GACNNNNNGTC 1 cut(s) 377
Eam1104I CTCTTC 1 cut(s) 736
Eam1105I GACNNNNNGTC 1 cut(s) 377
EarI CTCTTC 1 cut(s) 736
Eco105I TACGTA 1 cut(s) 861
Eco130I CCWWGG 1 cut(s) 771
Eco32I GATATC 2 cut(s) 270, 353
Eco47III AGCGCT 1 cut(s) 110
Eco88I CYCGRG 2 cut(s) 405, 679
Eco91I GGTNACC 1 cut(s) 379
EcoO65I GGTNACC 1 cut(s) 379
EcoRII CCWGG 1 cut(s) 18
EcoRV GATATC 2 cut(s) 270, 353
EcoT14I CCWWGG 1 cut(s) 771
EcoT22I ATGCAT 1 cut(s) 147
ErhI CCWWGG 1 cut(s) 771
FaeI CATG 3 cut(s) 184, 561, 730
FaqI GGGAC 1 cut(s) 624
FatI CATG 3 cut(s) 180, 557, 726
FbaI TGATCA 1 cut(s) 166
FblI GTMKAC 1 cut(s) 27
Fnu4HI GCNGC 5 cut(s) 125, 128, 131, 134, 804
FokI GGATG 5 cut(s) 222, 359, 470, 488, 788
Fsp4HI GCNGC 5 cut(s) 125, 128, 131, 134, 804
FspBI CTAG 1 cut(s) 281
GlaI GCGC 3 cut(s) 110, 137, 202
GluI GCNGC 5 cut(s) 125, 128, 131, 134, 804
HaeII RGCGCY 1 cut(s) 112
HapII CCGG 3 cut(s) 251, 650, 738
HhaI GCGC 3 cut(s) 111, 138, 203
Hin1II CATG 3 cut(s) 184, 561, 730
Hin6I GCGC 3 cut(s) 109, 136, 201
HinP1I GCGC 3 cut(s) 109, 136, 201
HincII GTYRAC 1 cut(s) 768
HindII GTYRAC 1 cut(s) 768
HinfI GANTC 4 cut(s) 81, 293, 479, 533
HpaI GTTAAC 1 cut(s) 768
HpaII CCGG 3 cut(s) 251, 650, 738
HphI GGTGA 2 cut(s) 373, 390
Hpy166II GTNNAC 3 cut(s) 28, 563, 768
Hpy188I TCNGA 1 cut(s) 292
Hpy188III TCNNGA 7 cut(s) 14, 38, 181, 239, 681, 695, 854
Hpy8I GTNNAC 3 cut(s) 28, 563, 768
HpyAV CCTTC 5 cut(s) 337, 370, 405, 448, 606
HpyCH4III ACNGT 3 cut(s) 32, 162, 276
HpyCH4IV ACGT 4 cut(s) 52, 624, 756, 860
HpyCH4V TGCA 4 cut(s) 49, 145, 468, 806
HpyF10VI GCNNNNNNNGC 6 cut(s) 130, 133, 142, 661, 723, 732
HpyF3I CTNAG 3 cut(s) 77, 105, 671
HpySE526I ACGT 4 cut(s) 52, 624, 756, 860
Hsp92II CATG 3 cut(s) 184, 561, 730
HspAI GCGC 3 cut(s) 109, 136, 201
Ksp22I TGATCA 1 cut(s) 166
KspAI GTTAAC 1 cut(s) 768
Kzo9I GATC 5 cut(s) 10, 166, 241, 667, 683
LmnI GCTCC 3 cut(s) 436, 652, 832
Lsp1109I GCAGC 5 cut(s) 120, 136, 139, 142, 790
LweI GCATC 3 cut(s) 244, 428, 766
MabI ACCWGGT 1 cut(s) 18
MaeI CTAG 1 cut(s) 281
MaeII ACGT 4 cut(s) 52, 624, 756, 860
MaeIII GTNAC 3 cut(s) 14, 379, 658
MalI GATC 5 cut(s) 12, 168, 243, 669, 685
MbiI CCGCTC 1 cut(s) 835
MboI GATC 5 cut(s) 10, 166, 241, 667, 683
MboII GAAGA 2 cut(s) 53, 753
MflI RGATCY 1 cut(s) 683
MhlI GDGCHC 2 cut(s) 513, 585
MluCI AATT 5 cut(s) 551, 576, 589, 781, 819
MlyI GAGTC 1 cut(s) 90
MmeI TCCRAC 1 cut(s) 175
Mph1103I ATGCAT 1 cut(s) 147
MseI TTAA 3 cut(s) 588, 630, 767
MslI CAYNNNNRTG 2 cut(s) 488, 578
MspA1I CMGCKG 1 cut(s) 133
MspI CCGG 3 cut(s) 251, 650, 738
MspR9I CCNGG 2 cut(s) 20, 738
MvaI CCWGG 1 cut(s) 20
MvnI CGCG 1 cut(s) 203
MwoI GCNNNNNNNGC 6 cut(s) 130, 133, 142, 661, 723, 732
NciI CCSGG 1 cut(s) 738
NdeII GATC 5 cut(s) 10, 166, 241, 667, 683
NlaIII CATG 3 cut(s) 184, 561, 730
NlaIV GGNNCC 2 cut(s) 654, 685
NmuCI GTSAC 2 cut(s) 14, 379
NsiI ATGCAT 1 cut(s) 147
PaeR7I CTCGAG 1 cut(s) 405
PagI TCATGA 1 cut(s) 180
PfeI GAWTC 3 cut(s) 293, 479, 533
PkrI GCNGC 5 cut(s) 126, 129, 132, 135, 805
PleI GAGTC 1 cut(s) 89
PpsI GAGTC 1 cut(s) 89
Ppu21I YACGTR 1 cut(s) 861
PshBI ATTAAT 1 cut(s) 630
PsiI TTATAA 1 cut(s) 333
Psp1406I AACGTT 1 cut(s) 52
Psp6I CCWGG 1 cut(s) 18
PspEI GGTNACC 1 cut(s) 379
PspGI CCWGG 1 cut(s) 18
PspN4I GGNNCC 2 cut(s) 654, 685
PspXI VCTCGAGB 1 cut(s) 405
PsrI GAACNNNNNNTAC 2 cut(s) 18, 50
PstI CTGCAG 1 cut(s) 808
PstNI CAGNNNCTG 1 cut(s) 133
PsuI RGATCY 1 cut(s) 683
PvuII CAGCTG 1 cut(s) 133
RsaI GTAC 2 cut(s) 459, 540
RsaNI GTAC 2 cut(s) 458, 539
RseI CAYNNNNRTG 2 cut(s) 488, 578
SaqAI TTAA 3 cut(s) 588, 630, 767
SatI GCNGC 5 cut(s) 125, 128, 131, 134, 804
Sau3AI GATC 5 cut(s) 10, 166, 241, 667, 683
SchI GAGTC 1 cut(s) 90
ScrFI CCNGG 2 cut(s) 20, 738
SduI GDGCHC 2 cut(s) 513, 585
SexAI ACCWGGT 1 cut(s) 18
SfaNI GCATC 3 cut(s) 244, 428, 766
SfcI CTRYAG 1 cut(s) 804
Sfr274I CTCGAG 1 cut(s) 405
SlaI CTCGAG 1 cut(s) 405
SmiMI CAYNNNNRTG 2 cut(s) 488, 578
SmlI CTYRAG 2 cut(s) 119, 405
SmoI CTYRAG 2 cut(s) 119, 405
SnaBI TACGTA 1 cut(s) 861
Sse9I AATT 5 cut(s) 551, 576, 589, 781, 819
SsiI CCGC 1 cut(s) 835
SspI AATATT 1 cut(s) 337
SspMI CTAG 1 cut(s) 281
StyD4I CCNGG 2 cut(s) 18, 736
StyI CCWWGG 1 cut(s) 771
TaaI ACNGT 3 cut(s) 32, 162, 276
TaiI ACGT 4 cut(s) 55, 627, 759, 863
TaqI TCGA 5 cut(s) 172, 406, 436, 666, 694
TasI AATT 5 cut(s) 551, 576, 589, 781, 819
TfiI GAWTC 3 cut(s) 293, 479, 533
Tru1I TTAA 3 cut(s) 588, 630, 767
Tru9I TTAA 3 cut(s) 588, 630, 767
TscAI CASTG 1 cut(s) 37
TseFI GTSAC 2 cut(s) 14, 379
TseI GCWGC 5 cut(s) 124, 127, 130, 133, 803
Tsp45I GTSAC 2 cut(s) 14, 379
TspDTI ATGAA 3 cut(s) 84, 546, 812
TspRI CASTG 1 cut(s) 37
VspI ATTAAT 1 cut(s) 630
XapI RAATTY 1 cut(s) 819
XhoI CTCGAG 1 cut(s) 405
XmiI GTMKAC 1 cut(s) 27
XspI CTAG 1 cut(s) 281
Zsp2I ATGCAT 1 cut(s) 147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.