Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr8

Orthogroup ID

OG0003631

Physical Location & Seq

Genomic Coordinates Reverse (-)

5137690 .. 5139018

Gene Structure 1329 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom08g06590.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1329 bp

ATGAAGTTTGATGCAGGAAGTGGTGGGCAAGTTCTAGATCTGGAAACTGCGGTGAAAGATGGAATTTTGGGCGGCGGAGGCGGGTTAATTTGCGGCGGCGGAGTTGGTGTTGGTGTTGTGGCGGATAAATTGGATCTGAAGAAGATGATTGAGGAGCTGGAGTCCATTGAGATTCCTTCAGTGTTCATCTGCCCGATTTCGTTGGAGCCAATGCAGGATCCGGTGACCCTTTGCACGGGTCAGACCTATGAGAGATCGAACATTCTCAAATGGTTCTCTTTGGGGCACTTTACTTGCCCCACAACAATGCAGGAGCTTTGGGATGATTCGGTCACGCCAAATATGACCCTCCACCAGCTGATTTGCAGCTGGTTTTCGCAGAAGTACGTGGCGATGAAGAAGCGGTCGGAGGACGTGCAGGGGAGGGCTCTTGAGATTTTGGACACATTGAAGAAGGTTAAGGGCCAAGCCAGAGTTCAAGCTCTCAAGGAGCTAAGAAGGGTAGTGGCAGCTCACGCTTCAGCTAAGAACACGGTGGTGGACAATGGGGGTGTTGCTCTGATCTCCTTTCTGTTGGGTCCTTTCACTTCGCATGCCGTTGGGTCGGAAGCAATTGGGATCCTTGTGAATTTGGAGCTCAGTTCAGAGTCCAGGACGAGTCTGCTGCAACCTGCAAAGGTTTCATTGATGGTTGATATGTTGAACGAGGGATCGATTGAGACGAAGATCAATTGCACGAAGTTGATTGAGGTTTTGATGGAGGGCAAGGATTTGGAGTCCGAAAACGTCTCAAGCTTGAGTCTTGTGGTTGGATTGTTGAGATTGGTCAAGGATAAAAGAAACCCTAATGGGGTTTTGCCCGGTCTCAGTTTGCTCAAGACAATATGTTCCCACGAATCAGTCAGGAACTCGGTTGTCAGCGTTGGGGCAGTTCCTCAATTGATTGAGCTCTTACCCGGTTTGAATCACGAGTGTTTGGAGGTAGCCCTTCATGTTCTAGAAGTTTTGTCAACACTTCCCGAGGGAAGGCAGGCTTTGAAAGATTGTCCCAAGACAATTCCCAGTGTAGTTAGACTACTGATGAAGGTTTCAGAGAGCTGCACTCAGCCCGCATTGTCGGTCTTGTGGGCGGTTTGCAAGCTAGCACCAGACGAATGCGCTCCTCTTGCGGTAGAGGCGGGTTTGGCAGCCAAGCTGCTGCTTGTGATTCAAAGTGGTTGCAATCCTGAGTTGAAGCAAAGAGCTGCTGAGCTCTTGAAGTTGTGTAGTCTAAATTATACCGCTACACATTTCATTTCGAAGTGTAAGCTTACCAGAACAATACAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009966 GO:0009987 GO:0010646 GO:0016020 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0023051 GO:0032446 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048583 GO:0050789 GO:0050794 GO:0061630 GO:0061659 GO:0065007 GO:0070647 GO:0071704 GO:0071944 GO:0140096 GO:1900457 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

KAP U-box

Protein Analysis

443

Amino Acids

47.58

Weight (kDa)

6.28

Isoelectric Point (pI)

43.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
U-box	PF04564	58 - 128	3e-15	U-box domain
ARM_PUB	PF25598	146 - 442	1.4e-88	Plant U-box protein ARM repeat region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003631)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G49810 AT5G65920 AT5G65920
fragaria_vesca	FvH4_1g18010 FvH4_1g18010 FvH4_1g18010 FvH4_2g32810
malus_domestica	MD08G1079800.v1.1 MD15G1066700.v1.1
prunus_persica	Prupe.1G420500_v2.0.a1 Prupe.6G218400_v2.0.a1
pyrus_communis	pycom08g06590 pycom15g06330
rosa_chinensis	RchiOBHm_Chr2g0108501 RchiOBHm_Chr6g0309891
rosa_laevigata	RLG00000010526 RLG00000017673
rosa_multiflora	Rmu_co8433595.1_g000001 Rmu_sc0000294.1_g000001 Rmu_sc0002372.1_g000052 Rmu_sc0009846.1_g000001
rosa_roxburghii	Rroxscaffold_2G00135090 Rroxscaffold_7G00159070
rosa_rugosa	Rorug02G0153700 Rorug06G0375700.1
rosa_samantha	Rh2AG202600 Rh2BG215500 Rh2CG206700 Rh2DG209800 Rh6AG489500 Rh6BG498900 Rh6CG503700 Rh6DG489900
rosa_wichuraiana	Rw2G016190 Rw6G042600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	679
AclWI	GGATC	6 cut(s)	141, 212, 225, 613, 626, 718
AcsI	RAATTY	2 cut(s)	63, 628
AcuI	CTGAAG	3 cut(s)	158, 162, 504
AfaI	GTAC	1 cut(s)	386
AfiI	CCNNNNNNNGG	3 cut(s)	235, 850, 1026
AgsI	TTSAA	8 cut(s)	451, 479, 703, 964, 1039, 1211, 1234, 1258
AjiI	CACGTC	1 cut(s)	415
AjnI	CCWGG	1 cut(s)	650
AjuI	GAANNNNNNNTTGG	2 cut(s)	459, 491
AleI	CACNNNNGTG	1 cut(s)	536
Alw21I	GWGCWC	3 cut(s)	639, 951, 1254
Alw26I	GTCTC	3 cut(s)	713, 793, 869
AlwI	GGATC	6 cut(s)	141, 212, 225, 613, 626, 718
Ama87I	CYCGRG	1 cut(s)	1019
AoxI	GGCC	1 cut(s)	463
ApeKI	GCWGC	8 cut(s)	366, 509, 664, 1098, 1187, 1195, 1198, 1244
ApoI	RAATTY	2 cut(s)	63, 628
AspLEI	GCGC	1 cut(s)	1160
AspS9I	GGNCC	2 cut(s)	463, 578
AsuC2I	CCSGG	2 cut(s)	861, 957
AsuHPI	GGTGA	2 cut(s)	64, 235
AsuII	TTCGAA	1 cut(s)	1298
AsuNHI	GCTAGC	1 cut(s)	1141
AvaI	CYCGRG	1 cut(s)	1019
AvaII	GGWCC	1 cut(s)	578
BaeGI	GKGCMC	1 cut(s)	288
BamHI	GGATCC	2 cut(s)	217, 618
BanII	GRGCYC	4 cut(s)	430, 639, 951, 1254
BauI	CACGAG	1 cut(s)	968
Bbv12I	GWGCWC	3 cut(s)	639, 951, 1254
BbvI	GCAGC	8 cut(s)	378, 521, 651, 1085, 1182, 1185, 1199, 1231
BccI	CCATC	3 cut(s)	53, 682, 751
BceAI	ACGGC	1 cut(s)	581
BcgI	CGANNNNNNTGC	2 cut(s)	646, 680
BciT130I	CCWGG	1 cut(s)	652
BcnI	CCSGG	2 cut(s)	861, 957
BcoDI	GTCTC	3 cut(s)	713, 793, 869
BfaI	CTAG	3 cut(s)	35, 998, 1142
BfuAI	ACCTGC	1 cut(s)	679
BglII	AGATCT	1 cut(s)	37
BlpI	GCTNAGC	1 cut(s)	1248
Bme1390I	CCNGG	3 cut(s)	652, 861, 957
Bme18I	GGWCC	1 cut(s)	578
BmeT110I	CYCGRG	1 cut(s)	1019
BmgBI	CACGTC	1 cut(s)	415
BmgT120I	GGNCC	2 cut(s)	463, 578
BmiI	GGNNCC	4 cut(s)	207, 219, 579, 620
BmrFI	CCNGG	3 cut(s)	652, 861, 957
BmrI	ACTGGG	1 cut(s)	1056
BmtI	GCTAGC	1 cut(s)	1145
BmuI	ACTGGG	1 cut(s)	1056
BplI	GAGNNNNNCTC	2 cut(s)	1087, 1119
BpmI	CTGGAG	1 cut(s)	179
Bpu1102I	GCTNAGC	1 cut(s)	1248
Bpu14I	TTCGAA	1 cut(s)	1298
BpuEI	CTTGAG	5 cut(s)	452, 470, 775, 817, 860
BpuMI	CCSGG	2 cut(s)	861, 957
Bsa29I	ATCGAT	1 cut(s)	713
BsaAI	YACGTR	1 cut(s)	388
BsaI	GGTCTC	1 cut(s)	869
BsaJI	CCNNGG	1 cut(s)	1020
BsaWI	WCCGGW	1 cut(s)	220
BsaXI	ACNNNNNCTCC	4 cut(s)	93, 123, 146, 176
Bsc4I	CCNNNNNNNGG	3 cut(s)	235, 850, 1026
Bse1I	ACTGG	1 cut(s)	1062
BseBI	CCWGG	1 cut(s)	652
BseCI	ATCGAT	1 cut(s)	713
BseDI	CCNNGG	1 cut(s)	1020
BseGI	GGATG	1 cut(s)	328
BseLI	CCNNNNNNNGG	3 cut(s)	235, 850, 1026
BseMII	CTCAG	5 cut(s)	652, 880, 1118, 1218, 1239
BseNI	ACTGG	1 cut(s)	1062
BseRI	GAGGAG	2 cut(s)	167, 1152
BseSI	GKGCMC	1 cut(s)	288
BseXI	GCAGC	8 cut(s)	378, 521, 651, 1085, 1182, 1185, 1199, 1231
BsgI	GTGCAG	2 cut(s)	437, 1084
Bsh1285I	CGRYCG	1 cut(s)	407
BshFI	GGCC	1 cut(s)	465
BshVI	ATCGAT	1 cut(s)	713
BsiEI	CGRYCG	1 cut(s)	407
BsiHKAI	GWGCWC	3 cut(s)	639, 951, 1254
BsiHKCI	CYCGRG	1 cut(s)	1019
BsiSI	CCGG	3 cut(s)	221, 861, 957
BslFI	GGGAC	1 cut(s)	1032
BslI	CCNNNNNNNGG	3 cut(s)	235, 850, 1026
BsmAI	GTCTC	3 cut(s)	713, 793, 869
BsmBI	CGTCTC	2 cut(s)	713, 793
BsmFI	GGGAC	1 cut(s)	1032
BsmI	GAATGC	1 cut(s)	1160
BsnI	GGCC	1 cut(s)	465
Bso31I	GGTCTC	1 cut(s)	869
BsoBI	CYCGRG	1 cut(s)	1019
Bsp119I	TTCGAA	1 cut(s)	1298
Bsp1286I	GDGCHC	5 cut(s)	288, 430, 639, 951, 1254
Bsp143I	GATC	8 cut(s)	37, 133, 217, 254, 561, 618, 710, 726
Bsp1720I	GCTNAGC	1 cut(s)	1248
BspANI	GGCC	1 cut(s)	465
BspCNI	CTCAG	5 cut(s)	651, 879, 1117, 1219, 1240
BspDI	ATCGAT	1 cut(s)	713
BspLI	GGNNCC	4 cut(s)	207, 219, 579, 620
BspMI	ACCTGC	1 cut(s)	679
BspOI	GCTAGC	1 cut(s)	1145
BspPI	GGATC	6 cut(s)	141, 212, 225, 613, 626, 718
BspT104I	TTCGAA	1 cut(s)	1298
BspTNI	GGTCTC	1 cut(s)	869
BsrI	ACTGG	1 cut(s)	1062
BssECI	CCNNGG	1 cut(s)	1020
BssMI	GATC	8 cut(s)	37, 133, 217, 254, 561, 618, 710, 726
BssSI	CACGAG	1 cut(s)	968
Bst2BI	CACGAG	1 cut(s)	968
Bst2UI	CCWGG	1 cut(s)	652
Bst4CI	ACNGT	1 cut(s)	535
BstBAI	YACGTR	1 cut(s)	388
BstBI	TTCGAA	1 cut(s)	1298
BstC8I	GCNNGC	5 cut(s)	594, 1032, 1110, 1139, 1143
BstDEI	CTNAG	7 cut(s)	494, 525, 638, 866, 1104, 1227, 1248
BstEII	GGTNACC	1 cut(s)	223
BstF5I	GGATG	1 cut(s)	328
BstHHI	GCGC	1 cut(s)	1160
BstKTI	GATC	8 cut(s)	40, 136, 220, 257, 564, 621, 713, 729
BstMAI	GTCTC	3 cut(s)	713, 793, 869
BstMBI	GATC	8 cut(s)	37, 133, 217, 254, 561, 618, 710, 726
BstMCI	CGRYCG	1 cut(s)	407
BstMWI	GCNNNNNNNGC	5 cut(s)	78, 515, 1166, 1175, 1184
BstNI	CCWGG	1 cut(s)	652
BstNSI	RCATGY	1 cut(s)	596
BstPI	GGTNACC	1 cut(s)	223
BstSCI	CCNGG	3 cut(s)	650, 859, 955
BstSLI	GKGCMC	1 cut(s)	288
BstV1I	GCAGC	8 cut(s)	378, 521, 651, 1085, 1182, 1185, 1199, 1231
BstX2I	RGATCY	4 cut(s)	37, 133, 217, 618
BstYI	RGATCY	4 cut(s)	37, 133, 217, 618
Bsu15I	ATCGAT	1 cut(s)	713
BsuRI	GGCC	1 cut(s)	465
BsuTUI	ATCGAT	1 cut(s)	713
BtgZI	GCGATG	1 cut(s)	407
BtrI	CACGTC	1 cut(s)	415
BtsCI	GGATG	1 cut(s)	328
BtsIMutI	CAGTG	2 cut(s)	186, 1069
BveI	ACCTGC	1 cut(s)	679
Cac8I	GCNNGC	5 cut(s)	594, 1032, 1110, 1139, 1143
CfoI	GCGC	1 cut(s)	1160
Cfr13I	GGNCC	2 cut(s)	463, 578
ClaI	ATCGAT	1 cut(s)	713
Csp6I	GTAC	1 cut(s)	385
CviAII	CATG	2 cut(s)	593, 992
CviQI	GTAC	1 cut(s)	385
DdeI	CTNAG	7 cut(s)	494, 525, 638, 866, 1104, 1227, 1248
DpnI	GATC	8 cut(s)	39, 135, 219, 256, 563, 620, 712, 728
DpnII	GATC	8 cut(s)	37, 133, 217, 254, 561, 618, 710, 726
EciI	GGCGGA	3 cut(s)	90, 114, 137
Ecl136II	GAGCTC	3 cut(s)	637, 949, 1252
Eco24I	GRGCYC	4 cut(s)	430, 639, 951, 1254
Eco31I	GGTCTC	1 cut(s)	869
Eco47I	GGWCC	1 cut(s)	578
Eco53kI	GAGCTC	3 cut(s)	637, 949, 1252
Eco57I	CTGAAG	3 cut(s)	158, 162, 504
Eco88I	CYCGRG	1 cut(s)	1019
Eco91I	GGTNACC	1 cut(s)	223
EcoICRI	GAGCTC	3 cut(s)	637, 949, 1252
EcoO109I	RGGNCCY	1 cut(s)	578
EcoO65I	GGTNACC	1 cut(s)	223
EcoRII	CCWGG	1 cut(s)	650
EcoT38I	GRGCYC	4 cut(s)	430, 639, 951, 1254
Esp3I	CGTCTC	2 cut(s)	713, 793
FaeI	CATG	2 cut(s)	596, 995
FaiI	YATR	7 cut(s)	249, 344, 594, 698, 886, 993, 1278
FalI	AAGNNNNNCTT	2 cut(s)	1018, 1050
FaqI	GGGAC	1 cut(s)	1032
FatI	CATG	2 cut(s)	592, 991
FauI	CCCGC	3 cut(s)	74, 1117, 1171
FokI	GGATG	1 cut(s)	335
FriOI	GRGCYC	4 cut(s)	430, 639, 951, 1254
FspBI	CTAG	3 cut(s)	35, 998, 1142
GlaI	GCGC	1 cut(s)	1159
GsuI	CTGGAG	1 cut(s)	179
HaeIII	GGCC	1 cut(s)	465
HapII	CCGG	3 cut(s)	221, 861, 957
HhaI	GCGC	1 cut(s)	1160
Hin1II	CATG	2 cut(s)	596, 995
Hin6I	GCGC	1 cut(s)	1158
HinP1I	GCGC	1 cut(s)	1158
HincII	GTYRAC	1 cut(s)	1011
HindII	GTYRAC	1 cut(s)	1011
HindIII	AAGCTT	2 cut(s)	793, 1307
HpaII	CCGG	3 cut(s)	221, 861, 957
HphI	GGTGA	2 cut(s)	64, 235
Hpy166II	GTNNAC	2 cut(s)	541, 1011
Hpy188I	TCNGA	8 cut(s)	138, 243, 409, 561, 607, 646, 781, 1093
Hpy8I	GTNNAC	2 cut(s)	541, 1011
HpyAV	CCTTC	6 cut(s)	186, 448, 492, 998, 1020, 1078
HpyCH4III	ACNGT	1 cut(s)	535
HpyCH4IV	ACGT	3 cut(s)	387, 414, 786
HpyF10VI	GCNNNNNNNGC	5 cut(s)	78, 515, 1166, 1175, 1184
HpyF3I	CTNAG	7 cut(s)	494, 525, 638, 866, 1104, 1227, 1248
HpySE526I	ACGT	3 cut(s)	387, 414, 786
Hsp92II	CATG	2 cut(s)	596, 995
HspAI	GCGC	1 cut(s)	1158
Kzo9I	GATC	8 cut(s)	37, 133, 217, 254, 561, 618, 710, 726
LmnI	GCTCC	6 cut(s)	154, 205, 313, 490, 634, 1165
Lsp1109I	GCAGC	8 cut(s)	378, 521, 651, 1085, 1182, 1185, 1199, 1231
MaeI	CTAG	3 cut(s)	35, 998, 1142
MaeII	ACGT	3 cut(s)	387, 414, 786
MaeIII	GTNAC	2 cut(s)	223, 331
MalI	GATC	8 cut(s)	39, 135, 219, 256, 563, 620, 712, 728
MboI	GATC	8 cut(s)	37, 133, 217, 254, 561, 618, 710, 726
MboII	GAAGA	5 cut(s)	151, 154, 409, 463, 736
MfeI	CAATTG	3 cut(s)	612, 730, 938
MflI	RGATCY	4 cut(s)	37, 133, 217, 618
MhlI	GDGCHC	5 cut(s)	288, 430, 639, 951, 1254
MluCI	AATT	9 cut(s)	63, 87, 128, 612, 628, 730, 938, 1056, 1273
MlyI	GAGTC	5 cut(s)	170, 656, 667, 785, 808
MmeI	TCCRAC	4 cut(s)	183, 387, 585, 790
MseI	TTAA	2 cut(s)	86, 459
MslI	CAYNNNNRTG	2 cut(s)	305, 536
MspA1I	CMGCKG	2 cut(s)	358, 369
MspI	CCGG	3 cut(s)	221, 861, 957
MspR9I	CCNGG	3 cut(s)	652, 861, 957
MunI	CAATTG	3 cut(s)	612, 730, 938
Mva1269I	GAATGC	1 cut(s)	1160
MvaI	CCWGG	1 cut(s)	652
MwoI	GCNNNNNNNGC	5 cut(s)	78, 515, 1166, 1175, 1184
NciI	CCSGG	2 cut(s)	861, 957
NdeII	GATC	8 cut(s)	37, 133, 217, 254, 561, 618, 710, 726
NheI	GCTAGC	1 cut(s)	1141
NlaIII	CATG	2 cut(s)	596, 995
NlaIV	GGNNCC	4 cut(s)	207, 219, 579, 620
NmuCI	GTSAC	2 cut(s)	223, 331
NspI	RCATGY	1 cut(s)	596
NspV	TTCGAA	1 cut(s)	1298
OliI	CACNNNNGTG	1 cut(s)	536
PaeI	GCATGC	1 cut(s)	596
PcsI	WCGNNNNNNNCGW	1 cut(s)	719
PctI	GAATGC	1 cut(s)	1160
PfeI	GAWTC	5 cut(s)	172, 326, 896, 964, 1207
PfoI	TCCNGGA	1 cut(s)	650
PleI	GAGTC	5 cut(s)	169, 655, 666, 784, 807
PpsI	GAGTC	5 cut(s)	169, 655, 666, 784, 807
Ppu21I	YACGTR	1 cut(s)	388
PpuMI	RGGWCCY	1 cut(s)	578
Psp124BI	GAGCTC	3 cut(s)	639, 951, 1254
Psp5II	RGGWCCY	1 cut(s)	578
Psp6I	CCWGG	1 cut(s)	650
PspEI	GGTNACC	1 cut(s)	223
PspGI	CCWGG	1 cut(s)	650
PspN4I	GGNNCC	4 cut(s)	207, 219, 579, 620
PspPI	GGNCC	2 cut(s)	463, 578
PspPPI	RGGWCCY	1 cut(s)	578
PsuI	RGATCY	4 cut(s)	37, 133, 217, 618
PvuII	CAGCTG	2 cut(s)	358, 369
RsaI	GTAC	1 cut(s)	386
RsaNI	GTAC	1 cut(s)	385
RseI	CAYNNNNRTG	2 cut(s)	305, 536
SacI	GAGCTC	3 cut(s)	639, 951, 1254
SaqAI	TTAA	2 cut(s)	86, 459
Sau3AI	GATC	8 cut(s)	37, 133, 217, 254, 561, 618, 710, 726
Sau96I	GGNCC	2 cut(s)	463, 578
SchI	GAGTC	5 cut(s)	170, 656, 667, 785, 808
ScrFI	CCNGG	3 cut(s)	652, 861, 957
SduI	GDGCHC	5 cut(s)	288, 430, 639, 951, 1254
SfuI	TTCGAA	1 cut(s)	1298
SinI	GGWCC	1 cut(s)	578
SmiMI	CAYNNNNRTG	2 cut(s)	305, 536
SmlI	CTYRAG	5 cut(s)	431, 485, 790, 796, 875
SmoI	CTYRAG	5 cut(s)	431, 485, 790, 796, 875
SphI	GCATGC	1 cut(s)	596
Sse9I	AATT	9 cut(s)	63, 87, 128, 612, 628, 730, 938, 1056, 1273
SspMI	CTAG	3 cut(s)	35, 998, 1142
SstI	GAGCTC	3 cut(s)	639, 951, 1254
StyD4I	CCNGG	3 cut(s)	650, 859, 955
TaaI	ACNGT	1 cut(s)	535
TaiI	ACGT	3 cut(s)	390, 417, 789
TaqI	TCGA	3 cut(s)	257, 713, 1298
TaqII	GACCGA	2 cut(s)	319, 1108
TasI	AATT	9 cut(s)	63, 87, 128, 612, 628, 730, 938, 1056, 1273
TauI	GCSGC	3 cut(s)	75, 96, 99
TfiI	GAWTC	5 cut(s)	172, 326, 896, 964, 1207
Tru1I	TTAA	2 cut(s)	86, 459
Tru9I	TTAA	2 cut(s)	86, 459
TscAI	CASTG	2 cut(s)	186, 1069
TseFI	GTSAC	2 cut(s)	223, 331
TseI	GCWGC	8 cut(s)	366, 509, 664, 1098, 1187, 1195, 1198, 1244
Tsp45I	GTSAC	2 cut(s)	223, 331
TspDTI	ATGAA	7 cut(s)	17, 175, 410, 672, 980, 1097, 1282
TspRI	CASTG	2 cut(s)	186, 1069
VpaK11BI	GGWCC	1 cut(s)	578
XapI	RAATTY	2 cut(s)	63, 628
XbaI	TCTAGA	2 cut(s)	34, 997
XceI	RCATGY	1 cut(s)	596
XspI	CTAG	3 cut(s)	35, 998, 1142

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.