pycom08g09780

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0010531
Physical Location & Seq
Forward (+)
8127682 .. 8129184
1503 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 714 bp
ATGGTAGGCAGTGCAAGGCTTTGCTTGCACAACTCCATGAGAGCTTTGAATTTATTTGTCAACGTTTGTGGAGTTGGAGTCATCATCTACTCCCTTTGGCTCATCAAGAAATGGCAAGTTGGAGTCTCTGAGCTCCCTCCCGTCTCGCATGTCCCCAAGCCATGGTTTATCTATACATGTTTAGGTATGGGAATTGCTGTCTGCCTGAGCACCCTTTGCGGTCATATGGTTGCCAATTCCATCAGCAGTTTTACTCTCTTCATTTACATAATCTCCATCTTCTGTCTTCTTTGCCTCGAAGTTGGAGTGATTATCAACATTTTCTATAGGATGGACTGGGAAGTGCAATTTGTAAAGTACATTGATGAGGATCACACCGTGTTCAAAAATTTCTTGATGTTTCATCTAAACGTGTGCCGCCTCATTGTAATTATCATCTTGATACCACAGATCAAAGCTATTGTGCTGGCGATCATTCTTTGGGCCATTGGTTCCGAGCCGGGGGCGGATCAATGCAACTATTCTGATGTCACAAACTTCAGATATTCGTTTCTGGCAGCGCCTACACAATCTTTACTAGATATATGGCGACATGGATTCCGAAACTATGAAGTTTCGTCAAGAGAATTCGAAAACCCGCCACGCCCGAGTTTTGTTTCAAATGCACAGAGATTTTTCAGAATGCATTTTCACAGAAGAGTCACTCTTAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tetraspannin

Protein Analysis

238

Amino Acids

27.26

Weight (kDa)

8.76

Isoelectric Point (pI)

29.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tetraspanin PF00335 13 - 113 8.4e-07 Tetraspanin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 162
AciI CCGC 4 cut(s) 219, 418, 506, 638
AclI AACGTT 1 cut(s) 63
AclWI GGATC 2 cut(s) 378, 516
AcsI RAATTY 3 cut(s) 49, 388, 626
AcuI CTGAAG 1 cut(s) 523
AdeI CACNNNGTG 1 cut(s) 379
AfaI GTAC 1 cut(s) 359
AfiI CCNNNNNNNGG 2 cut(s) 162, 501
AflIII ACRYGT 2 cut(s) 176, 411
AgsI TTSAA 3 cut(s) 49, 385, 660
AluBI AGCT 3 cut(s) 44, 133, 458
AluI AGCT 3 cut(s) 44, 133, 458
Alw21I GWGCWC 2 cut(s) 135, 212
Alw26I GTCTC 2 cut(s) 130, 148
AlwI GGATC 2 cut(s) 378, 516
Ama87I CYCGRG 1 cut(s) 646
AoxI GGCC 1 cut(s) 483
ApeKI GCWGC 1 cut(s) 557
ApoI RAATTY 3 cut(s) 49, 388, 626
AspLEI GCGC 1 cut(s) 562
AspS9I GGNCC 1 cut(s) 483
AsuC2I CCSGG 1 cut(s) 501
AsuII TTCGAA 1 cut(s) 630
AvaI CYCGRG 1 cut(s) 646
BanII GRGCYC 1 cut(s) 135
BbsI GAAGAC 1 cut(s) 278
Bbv12I GWGCWC 2 cut(s) 135, 212
BbvI GCAGC 1 cut(s) 569
BccI CCATC 3 cut(s) 248, 284, 325
BcnI CCSGG 1 cut(s) 501
BcoDI GTCTC 2 cut(s) 130, 148
BfaI CTAG 1 cut(s) 578
BfmI CTRYAG 1 cut(s) 325
BfoI RGCGCY 1 cut(s) 563
BisI GCNGC 2 cut(s) 418, 558
BlsI GCNGC 2 cut(s) 419, 559
Bme1390I CCNGG 1 cut(s) 501
BmeT110I CYCGRG 1 cut(s) 646
BmgT120I GGNCC 1 cut(s) 483
BmiI GGNNCC 1 cut(s) 493
BmrFI CCNGG 1 cut(s) 501
BmrI ACTGGG 1 cut(s) 346
BmuI ACTGGG 1 cut(s) 346
BpiI GAAGAC 1 cut(s) 278
Bpu10I CCTNAGC 1 cut(s) 206
Bpu14I TTCGAA 1 cut(s) 630
BpuMI CCSGG 1 cut(s) 501
BsaBI GATNNNNATC 1 cut(s) 369
BsaJI CCNNGG 2 cut(s) 161, 500
BsaXI ACNNNNNCTCC 4 cut(s) 63, 93, 257, 287
Bsc4I CCNNNNNNNGG 2 cut(s) 162, 501
Bse1I ACTGG 1 cut(s) 341
Bse8I GATNNNNATC 1 cut(s) 369
BseDI CCNNGG 2 cut(s) 161, 500
BseGI GGATG 1 cut(s) 336
BseJI GATNNNNATC 1 cut(s) 369
BseLI CCNNNNNNNGG 2 cut(s) 162, 501
BseMII CTCAG 2 cut(s) 120, 197
BseNI ACTGG 1 cut(s) 341
BseXI GCAGC 1 cut(s) 569
BshFI GGCC 1 cut(s) 485
BsiHKAI GWGCWC 2 cut(s) 135, 212
BsiHKCI CYCGRG 1 cut(s) 646
BsiSI CCGG 1 cut(s) 500
BslFI GGGAC 1 cut(s) 137
BslI CCNNNNNNNGG 2 cut(s) 162, 501
BsmAI GTCTC 2 cut(s) 130, 148
BsmBI CGTCTC 1 cut(s) 148
BsmFI GGGAC 1 cut(s) 137
BsmI GAATGC 1 cut(s) 687
BsnI GGCC 1 cut(s) 485
BsoBI CYCGRG 1 cut(s) 646
Bsp119I TTCGAA 1 cut(s) 630
Bsp1286I GDGCHC 2 cut(s) 135, 212
Bsp143I GATC 4 cut(s) 370, 450, 471, 508
Bsp19I CCATGG 1 cut(s) 161
BspACI CCGC 4 cut(s) 219, 418, 506, 638
BspANI GGCC 1 cut(s) 485
BspCNI CTCAG 2 cut(s) 121, 198
BspLI GGNNCC 1 cut(s) 493
BspPI GGATC 2 cut(s) 378, 516
BspT104I TTCGAA 1 cut(s) 630
BsrI ACTGG 1 cut(s) 341
BssECI CCNNGG 2 cut(s) 161, 500
BssMI GATC 4 cut(s) 370, 450, 471, 508
BssT1I CCWWGG 1 cut(s) 161
Bst4CI ACNGT 1 cut(s) 379
Bst6I CTCTTC 2 cut(s) 263, 691
BstAPI GCANNNNNTGC 1 cut(s) 216
BstBI TTCGAA 1 cut(s) 630
BstC8I GCNNGC 2 cut(s) 26, 468
BstDEI CTNAG 3 cut(s) 129, 206, 707
BstDSI CCRYGG 1 cut(s) 161
BstF5I GGATG 1 cut(s) 336
BstH2I RGCGCY 1 cut(s) 563
BstHHI GCGC 1 cut(s) 562
BstKTI GATC 4 cut(s) 373, 453, 474, 511
BstMAI GTCTC 2 cut(s) 130, 148
BstMBI GATC 4 cut(s) 370, 450, 471, 508
BstMWI GCNNNNNNNGC 2 cut(s) 25, 216
BstNSI RCATGY 2 cut(s) 152, 180
BstSCI CCNGG 1 cut(s) 499
BstSFI CTRYAG 1 cut(s) 325
BstV1I GCAGC 1 cut(s) 569
BstV2I GAAGAC 1 cut(s) 278
BsuRI GGCC 1 cut(s) 485
BtgI CCRYGG 1 cut(s) 161
BtsCI GGATG 1 cut(s) 336
BtsI GCAGTG 1 cut(s) 16
BtsIMutI CAGTG 1 cut(s) 16
Cac8I GCNNGC 2 cut(s) 26, 468
CfoI GCGC 1 cut(s) 562
Cfr13I GGNCC 1 cut(s) 483
Csp6I GTAC 1 cut(s) 358
CspCI CAANNNNNGTGG 2 cut(s) 49, 84
CviAII CATG 5 cut(s) 37, 149, 162, 177, 593
CviJI RGCY 8 cut(s) 19, 44, 100, 133, 160, 458, 485, 499
CviKI_1 RGCY 8 cut(s) 19, 44, 100, 133, 160, 458, 485, 499
CviQI GTAC 1 cut(s) 358
DdeI CTNAG 3 cut(s) 129, 206, 707
DpnI GATC 4 cut(s) 372, 452, 473, 510
DpnII GATC 4 cut(s) 370, 450, 471, 508
DraIII CACNNNGTG 1 cut(s) 379
Eam1104I CTCTTC 2 cut(s) 263, 691
EarI CTCTTC 2 cut(s) 263, 691
EciI GGCGGA 1 cut(s) 521
Ecl136II GAGCTC 1 cut(s) 133
Eco130I CCWWGG 1 cut(s) 161
Eco24I GRGCYC 1 cut(s) 135
Eco53kI GAGCTC 1 cut(s) 133
Eco57I CTGAAG 1 cut(s) 523
Eco88I CYCGRG 1 cut(s) 646
EcoICRI GAGCTC 1 cut(s) 133
EcoRI GAATTC 1 cut(s) 626
EcoT14I CCWWGG 1 cut(s) 161
EcoT22I ATGCAT 1 cut(s) 687
EcoT38I GRGCYC 1 cut(s) 135
ErhI CCWWGG 1 cut(s) 161
Esp3I CGTCTC 1 cut(s) 148
FaeI CATG 5 cut(s) 40, 152, 165, 180, 596
FaqI GGGAC 1 cut(s) 137
FatI CATG 5 cut(s) 36, 148, 161, 176, 592
FauI CCCGC 1 cut(s) 645
FauNDI CATATG 1 cut(s) 225
Fnu4HI GCNGC 2 cut(s) 418, 558
FokI GGATG 1 cut(s) 343
FriOI GRGCYC 1 cut(s) 135
Fsp4HI GCNGC 2 cut(s) 418, 558
FspBI CTAG 1 cut(s) 578
GlaI GCGC 1 cut(s) 561
GluI GCNGC 2 cut(s) 418, 558
HaeII RGCGCY 1 cut(s) 563
HaeIII GGCC 1 cut(s) 485
HapII CCGG 1 cut(s) 500
HhaI GCGC 1 cut(s) 562
Hin1II CATG 5 cut(s) 40, 152, 165, 180, 596
Hin6I GCGC 1 cut(s) 560
HinP1I GCGC 1 cut(s) 560
HincII GTYRAC 1 cut(s) 61
HindII GTYRAC 1 cut(s) 61
HinfI GANTC 4 cut(s) 78, 123, 597, 699
HpaII CCGG 1 cut(s) 500
Hpy166II GTNNAC 1 cut(s) 61
Hpy188I TCNGA 6 cut(s) 130, 496, 526, 542, 602, 680
Hpy188III TCNNGA 4 cut(s) 106, 394, 439, 621
Hpy8I GTNNAC 1 cut(s) 61
HpyCH4III ACNGT 1 cut(s) 379
HpyCH4IV ACGT 2 cut(s) 63, 411
HpyCH4V TGCA 6 cut(s) 14, 28, 346, 516, 665, 685
HpyF10VI GCNNNNNNNGC 2 cut(s) 25, 216
HpyF3I CTNAG 3 cut(s) 129, 206, 707
HpySE526I ACGT 2 cut(s) 63, 411
Hsp92II CATG 5 cut(s) 40, 152, 165, 180, 596
HspAI GCGC 1 cut(s) 560
Kzo9I GATC 4 cut(s) 370, 450, 471, 508
LmnI GCTCC 1 cut(s) 138
LpnPI CCDG 5 cut(s) 218, 322, 452, 513, 539
Lsp1109I GCAGC 1 cut(s) 569
MaeI CTAG 1 cut(s) 578
MaeII ACGT 2 cut(s) 63, 411
MaeIII GTNAC 2 cut(s) 529, 700
MalI GATC 4 cut(s) 372, 452, 473, 510
MboI GATC 4 cut(s) 370, 450, 471, 508
MboII GAAGA 4 cut(s) 250, 271, 278, 708
MhlI GDGCHC 2 cut(s) 135, 212
MluCI AATT 7 cut(s) 49, 192, 235, 347, 388, 429, 626
MlyI GAGTC 3 cut(s) 87, 132, 708
MmeI TCCRAC 3 cut(s) 55, 100, 283
MnlI CCTC 4 cut(s) 147, 305, 361, 431
Mph1103I ATGCAT 1 cut(s) 687
MspI CCGG 1 cut(s) 500
MspR9I CCNGG 1 cut(s) 501
Mva1269I GAATGC 1 cut(s) 687
MwoI GCNNNNNNNGC 2 cut(s) 25, 216
NciI CCSGG 1 cut(s) 501
NcoI CCATGG 1 cut(s) 161
NdeI CATATG 1 cut(s) 225
NdeII GATC 4 cut(s) 370, 450, 471, 508
NlaIII CATG 5 cut(s) 40, 152, 165, 180, 596
NlaIV GGNNCC 1 cut(s) 493
NmuCI GTSAC 2 cut(s) 529, 700
NsiI ATGCAT 1 cut(s) 687
NspI RCATGY 2 cut(s) 152, 180
NspV TTCGAA 1 cut(s) 630
PciI ACATGT 1 cut(s) 176
PctI GAATGC 1 cut(s) 687
PfeI GAWTC 1 cut(s) 597
PflMI CCANNNNNTGG 1 cut(s) 162
PkrI GCNGC 2 cut(s) 419, 559
PleI GAGTC 3 cut(s) 86, 131, 707
PpsI GAGTC 3 cut(s) 86, 131, 707
PscI ACATGT 1 cut(s) 176
Psp124BI GAGCTC 1 cut(s) 135
Psp1406I AACGTT 1 cut(s) 63
PspN4I GGNNCC 1 cut(s) 493
PspPI GGNCC 1 cut(s) 483
RsaI GTAC 1 cut(s) 359
RsaNI GTAC 1 cut(s) 358
SacI GAGCTC 1 cut(s) 135
SatI GCNGC 2 cut(s) 418, 558
Sau3AI GATC 4 cut(s) 370, 450, 471, 508
Sau96I GGNCC 1 cut(s) 483
SchI GAGTC 3 cut(s) 87, 132, 708
ScrFI CCNGG 1 cut(s) 501
SduI GDGCHC 2 cut(s) 135, 212
SetI ASST 6 cut(s) 46, 66, 135, 187, 414, 460
SfcI CTRYAG 1 cut(s) 325
SfuI TTCGAA 1 cut(s) 630
Sse9I AATT 7 cut(s) 49, 192, 235, 347, 388, 429, 626
SsiI CCGC 4 cut(s) 219, 418, 506, 638
SspMI CTAG 1 cut(s) 578
SstI GAGCTC 1 cut(s) 135
StyD4I CCNGG 1 cut(s) 499
StyI CCWWGG 1 cut(s) 161
TaaI ACNGT 1 cut(s) 379
TaiI ACGT 2 cut(s) 66, 414
TaqI TCGA 2 cut(s) 297, 630
TasI AATT 7 cut(s) 49, 192, 235, 347, 388, 429, 626
TatI WGTACW 1 cut(s) 357
TauI GCSGC 1 cut(s) 420
TfiI GAWTC 1 cut(s) 597
TscAI CASTG 1 cut(s) 16
TseFI GTSAC 2 cut(s) 529, 700
TseI GCWGC 1 cut(s) 557
Tsp45I GTSAC 2 cut(s) 529, 700
TspDTI ATGAA 3 cut(s) 250, 392, 624
TspRI CASTG 1 cut(s) 16
Van91I CCANNNNNTGG 1 cut(s) 162
XapI RAATTY 3 cut(s) 49, 388, 626
XceI RCATGY 2 cut(s) 152, 180
XspI CTAG 1 cut(s) 578
Zsp2I ATGCAT 1 cut(s) 687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.