pycom08g11380

zinc-finger of the FCS-type, C2-C2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0006492
Physical Location & Seq
Forward (+)
9641891 .. 9643148
1258 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1152 bp
ATGGGTCATCTGCCAATTTCTGATGCTGCTGCTGGGTCTGATGTTTTAGGGCACAACCCTAAAAGCAACTCTTTTTTCAGTGTTCCTGGTCTGTTTGTGGGGTTGAGTCATAAGGGTTTATCAGATTATGATGCAGTTAGGAGCCCAACTTCTCCTCTAGATTTTAGGGTCTTTTCCAATCTAGGTTACCCCTTTAGGTCCCCAAGGTCCAACCATGATGGGGGGCAGCAGAGGAGCTGGGGTAGCAGTAAAGTAGGATTAAGCATCATAGATTCTATTGATGATGTGAAAATTTTCGGGAAAGTTCCTCAGTCATCTGAGAGTAAGAACATTCTTTTTGGACCCGGAATGAGGATTAATAAAATCCCAGAGCCTCAGTCCAATGCCAAGTGTTTTGATTATTCACCGAAATCTCTGCCTAAGAACTATGCAATTTCCCCTCATTCCAAAATCAAATCTCCTTTACAGAAGGGTAGTTCTGATGTTCTTTTCGAAATCGGAGAAGCCCCATCAGAACCTGAGTCATTCGGGAAAATCCGGTCCTGTTCATTAGATTCTGGTAGAGCATTTTCGACCCTATCAGGATTGAGCAACCTTAACCCCAACTCCACTTCTGGAGATTTTTGCCCGGAAAATTTAACCACCCATCAAGTTATTGGAGGAAGCCCCAATTTAGCCACCCAAATGAAAACTGGATCCATCGAGTCTAGCAATGGGTTTGTCGGGTCTCTTTCAGCAAGTGAGATTGAGCTTTCTGAGGATTATACTTGTGTAATCTCCCATGGTGCCAATCCCAAAAAAACTCACATCTTTGGTGACTGCATTTTGGAATGTCACTCTGAGGATGTGAACAATTTTGGTAAGAATGTGGAAGAGGAAATTGGATTTGCCCACCCGGGTTCGAGCTTGGAGAATTTTGGTCACTACCCTTCAGACAATTTCCTGAGCTTCTGTTACTATTGTAACAAGAAATTGGAAGAGGGCAAAGATATATACATATACAGGGGTGAGAAAGCATTTTGCAGTTTGAGTTGCCGGTCGGAGGAAATTTTGATTGATGAGGAGTTGGAAAAATGCAATGACCAGTCTTCTGGGAAACCTCTCGAGTCGAATGAGGAACTTTTTGAAACTGGCTTGCTTGCCGATAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-FLZ

Protein Analysis

384

Amino Acids

41.57

Weight (kDa)

5.21

Isoelectric Point (pI)

60.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-FLZ PF04570 308 - 355 6.7e-22 zinc-finger of the FCS-type, C2-C2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 785
AclWI GGATC 2 cut(s) 690, 703
AcsI RAATTY 4 cut(s) 291, 634, 913, 1047
AcuI CTGAAG 1 cut(s) 915
AfiI CCNNNNNNNGG 3 cut(s) 220, 351, 1042
AgsI TTSAA 1 cut(s) 1127
AjnI CCWGG 1 cut(s) 85
AjuI GAANNNNNNNTTGG 2 cut(s) 864, 896
AluBI AGCT 4 cut(s) 237, 751, 906, 948
AluI AGCT 4 cut(s) 237, 751, 906, 948
Alw26I GTCTC 1 cut(s) 732
AlwI GGATC 2 cut(s) 690, 703
AlwNI CAGNNNCTG 1 cut(s) 518
Ama87I CYCGRG 2 cut(s) 895, 1103
ApeKI GCWGC 3 cut(s) 26, 29, 226
ApoI RAATTY 4 cut(s) 291, 634, 913, 1047
AseI ATTAAT 1 cut(s) 357
Asp700I GAANNNNTTC 1 cut(s) 293
AspS9I GGNCC 4 cut(s) 198, 207, 341, 540
AsuC2I CCSGG 4 cut(s) 345, 629, 896, 897
AsuHPI GGTGA 3 cut(s) 396, 827, 1019
AsuII TTCGAA 1 cut(s) 492
AvaI CYCGRG 2 cut(s) 895, 1103
AvaII GGWCC 4 cut(s) 198, 207, 341, 540
BaeGI GKGCMC 1 cut(s) 54
BamHI GGATCC 1 cut(s) 695
BanI GGYRCC 1 cut(s) 785
BanII GRGCYC 1 cut(s) 146
BbsI GAAGAC 1 cut(s) 1080
BbvI GCAGC 3 cut(s) 13, 16, 238
BccI CCATC 4 cut(s) 212, 517, 654, 707
BcgI CGANNNNNNTGC 2 cut(s) 397, 431
BciT130I CCWGG 1 cut(s) 87
BcnI CCSGG 4 cut(s) 345, 629, 896, 897
BcoDI GTCTC 1 cut(s) 732
BfaI CTAG 3 cut(s) 158, 182, 708
BisI GCNGC 3 cut(s) 27, 30, 227
BlsI GCNGC 3 cut(s) 28, 31, 228
Bme1390I CCNGG 5 cut(s) 87, 345, 629, 896, 897
Bme18I GGWCC 4 cut(s) 198, 207, 341, 540
BmeT110I CYCGRG 2 cut(s) 895, 1103
BmgT120I GGNCC 4 cut(s) 198, 207, 341, 540
BmiI GGNNCC 5 cut(s) 143, 200, 343, 697, 787
BmrFI CCNGG 5 cut(s) 87, 345, 629, 896, 897
BmsI GCATC 3 cut(s) 13, 121, 273
BpiI GAAGAC 1 cut(s) 1080
BpmI CTGGAG 1 cut(s) 636
Bpu10I CCTNAGC 1 cut(s) 944
Bpu14I TTCGAA 1 cut(s) 492
BpuMI CCSGG 4 cut(s) 345, 629, 896, 897
BsaI GGTCTC 1 cut(s) 732
BsaJI CCNNGG 3 cut(s) 203, 781, 895
BsaWI WCCGGW 1 cut(s) 537
BsaXI ACNNNNNCTCC 4 cut(s) 226, 256, 590, 620
Bsc4I CCNNNNNNNGG 3 cut(s) 220, 351, 1042
Bse118I RCCGGY 1 cut(s) 1035
Bse1I ACTGG 3 cut(s) 697, 1084, 1135
Bse3DI GCAATG 2 cut(s) 718, 1084
BseBI CCWGG 1 cut(s) 87
BseDI CCNNGG 3 cut(s) 203, 781, 895
BseGI GGATG 1 cut(s) 850
BseLI CCNNNNNNNGG 3 cut(s) 220, 351, 1042
BseMI GCAATG 2 cut(s) 718, 1084
BseMII CTCAG 7 cut(s) 309, 323, 389, 510, 747, 831, 935
BseNI ACTGG 3 cut(s) 697, 1084, 1135
BseRI GAGGAG 3 cut(s) 144, 247, 1076
BseSI GKGCMC 1 cut(s) 54
BseXI GCAGC 3 cut(s) 13, 16, 238
BseYI CCCAGC 2 cut(s) 32, 237
Bsh1285I CGRYCG 1 cut(s) 1040
BshNI GGYRCC 1 cut(s) 785
BsiEI CGRYCG 1 cut(s) 1040
BsiHKCI CYCGRG 2 cut(s) 895, 1103
BsiSI CCGG 5 cut(s) 345, 538, 629, 896, 1036
BslFI GGGAC 1 cut(s) 184
BslI CCNNNNNNNGG 3 cut(s) 220, 351, 1042
BsmAI GTCTC 1 cut(s) 732
BsmFI GGGAC 1 cut(s) 184
Bso31I GGTCTC 1 cut(s) 732
BsoBI CYCGRG 2 cut(s) 895, 1103
Bsp119I TTCGAA 1 cut(s) 492
Bsp1286I GDGCHC 2 cut(s) 54, 146
Bsp143I GATC 1 cut(s) 695
Bsp19I CCATGG 1 cut(s) 781
BspCNI CTCAG 7 cut(s) 310, 322, 388, 511, 748, 832, 936
BspLI GGNNCC 5 cut(s) 143, 200, 343, 697, 787
BspPI GGATC 2 cut(s) 690, 703
BspT104I TTCGAA 1 cut(s) 492
BspT107I GGYRCC 1 cut(s) 785
BspTNI GGTCTC 1 cut(s) 732
BsrDI GCAATG 2 cut(s) 718, 1084
BsrFI RCCGGY 1 cut(s) 1035
BsrI ACTGG 3 cut(s) 697, 1084, 1135
BssAI RCCGGY 1 cut(s) 1035
BssECI CCNNGG 3 cut(s) 203, 781, 895
BssMI GATC 1 cut(s) 695
BssT1I CCWWGG 2 cut(s) 203, 781
Bst2UI CCWGG 1 cut(s) 87
Bst6I CTCTTC 2 cut(s) 867, 972
BstBI TTCGAA 1 cut(s) 492
BstC8I GCNNGC 2 cut(s) 1136, 1140
BstDEI CTNAG 8 cut(s) 309, 318, 375, 420, 519, 756, 840, 944
BstDSI CCRYGG 1 cut(s) 781
BstEII GGTNACC 1 cut(s) 185
BstF5I GGATG 1 cut(s) 850
BstKTI GATC 1 cut(s) 698
BstMAI GTCTC 1 cut(s) 732
BstMBI GATC 1 cut(s) 695
BstMCI CGRYCG 1 cut(s) 1040
BstMWI GCNNNNNNNGC 1 cut(s) 243
BstNI CCWGG 1 cut(s) 87
BstPI GGTNACC 1 cut(s) 185
BstSCI CCNGG 5 cut(s) 85, 343, 627, 894, 895
BstSLI GKGCMC 1 cut(s) 54
BstV1I GCAGC 3 cut(s) 13, 16, 238
BstV2I GAAGAC 1 cut(s) 1080
BstX2I RGATCY 1 cut(s) 695
BstXI CCANNNNNNTGG 1 cut(s) 1091
BstYI RGATCY 1 cut(s) 695
BtgI CCRYGG 1 cut(s) 781
BtsCI GGATG 1 cut(s) 850
BtsIMutI CAGTG 1 cut(s) 85
Cac8I GCNNGC 2 cut(s) 1136, 1140
CaiI CAGNNNCTG 1 cut(s) 518
Cfr10I RCCGGY 1 cut(s) 1035
Cfr13I GGNCC 4 cut(s) 198, 207, 341, 540
Cfr9I CCCGGG 1 cut(s) 895
CviAII CATG 2 cut(s) 215, 782
DdeI CTNAG 8 cut(s) 309, 318, 375, 420, 519, 756, 840, 944
DpnI GATC 1 cut(s) 697
DpnII GATC 1 cut(s) 695
Eam1104I CTCTTC 2 cut(s) 867, 972
EarI CTCTTC 2 cut(s) 867, 972
Eco130I CCWWGG 2 cut(s) 203, 781
Eco24I GRGCYC 1 cut(s) 146
Eco31I GGTCTC 1 cut(s) 732
Eco47I GGWCC 4 cut(s) 198, 207, 341, 540
Eco57I CTGAAG 1 cut(s) 915
Eco88I CYCGRG 2 cut(s) 895, 1103
Eco91I GGTNACC 1 cut(s) 185
EcoO109I RGGNCCY 1 cut(s) 198
EcoO65I GGTNACC 1 cut(s) 185
EcoRII CCWGG 1 cut(s) 85
EcoT14I CCWWGG 2 cut(s) 203, 781
EcoT38I GRGCYC 1 cut(s) 146
ErhI CCWWGG 2 cut(s) 203, 781
FaeI CATG 2 cut(s) 218, 785
FalI AAGNNNNNCTT 2 cut(s) 55, 87
FaqI GGGAC 1 cut(s) 184
FatI CATG 2 cut(s) 214, 781
Fnu4HI GCNGC 3 cut(s) 27, 30, 227
FokI GGATG 1 cut(s) 857
FriOI GRGCYC 1 cut(s) 146
Fsp4HI GCNGC 3 cut(s) 27, 30, 227
FspBI CTAG 3 cut(s) 158, 182, 708
GluI GCNGC 3 cut(s) 27, 30, 227
GsaI CCCAGC 2 cut(s) 36, 241
GsuI CTGGAG 1 cut(s) 636
HapII CCGG 5 cut(s) 345, 538, 629, 896, 1036
Hin1II CATG 2 cut(s) 218, 785
HinfI GANTC 6 cut(s) 106, 272, 521, 554, 704, 1106
HpaII CCGG 5 cut(s) 345, 538, 629, 896, 1036
HphI GGTGA 3 cut(s) 396, 827, 1019
Hpy166II GTNNAC 1 cut(s) 850
Hpy188III TCNNGA 7 cut(s) 158, 298, 529, 582, 615, 943, 1103
Hpy8I GTNNAC 1 cut(s) 850
HpyAV CCTTC 2 cut(s) 463, 939
HpyCH4V TGCA 5 cut(s) 134, 431, 822, 1023, 1077
HpyF10VI GCNNNNNNNGC 1 cut(s) 243
HpyF3I CTNAG 8 cut(s) 309, 318, 375, 420, 519, 756, 840, 944
Hsp92II CATG 2 cut(s) 218, 785
Kzo9I GATC 1 cut(s) 695
LmnI GCTCC 2 cut(s) 141, 234
Lsp1109I GCAGC 3 cut(s) 13, 16, 238
LweI GCATC 3 cut(s) 13, 121, 273
MaeI CTAG 3 cut(s) 158, 182, 708
MaeIII GTNAC 6 cut(s) 185, 815, 833, 920, 953, 962
MalI GATC 1 cut(s) 697
MboI GATC 1 cut(s) 695
MboII GAAGA 3 cut(s) 884, 989, 1080
MflI RGATCY 1 cut(s) 695
MhlI GDGCHC 2 cut(s) 54, 146
MlyI GAGTC 4 cut(s) 115, 530, 713, 1115
MmeI TCCRAC 3 cut(s) 234, 1020, 1047
MroXI GAANNNNTTC 1 cut(s) 293
MseI TTAA 4 cut(s) 260, 357, 597, 638
MslI CAYNNNNRTG 1 cut(s) 683
MspI CCGG 5 cut(s) 345, 538, 629, 896, 1036
MspR9I CCNGG 5 cut(s) 87, 345, 629, 896, 897
MvaI CCWGG 1 cut(s) 87
MwoI GCNNNNNNNGC 1 cut(s) 243
NciI CCSGG 4 cut(s) 345, 629, 896, 897
NcoI CCATGG 1 cut(s) 781
NdeII GATC 1 cut(s) 695
NlaIII CATG 2 cut(s) 218, 785
NlaIV GGNNCC 5 cut(s) 143, 200, 343, 697, 787
NmuCI GTSAC 3 cut(s) 815, 833, 920
NspV TTCGAA 1 cut(s) 492
PaeR7I CTCGAG 1 cut(s) 1103
PdmI GAANNNNTTC 1 cut(s) 293
PfeI GAWTC 2 cut(s) 272, 554
PkrI GCNGC 3 cut(s) 28, 31, 228
PleI GAGTC 4 cut(s) 114, 529, 712, 1114
PpsI GAGTC 4 cut(s) 114, 529, 712, 1114
PpuMI RGGWCCY 1 cut(s) 198
PshBI ATTAAT 1 cut(s) 357
Psp5II RGGWCCY 1 cut(s) 198
Psp6I CCWGG 1 cut(s) 85
PspEI GGTNACC 1 cut(s) 185
PspFI CCCAGC 2 cut(s) 32, 237
PspGI CCWGG 1 cut(s) 85
PspN4I GGNNCC 5 cut(s) 143, 200, 343, 697, 787
PspPI GGNCC 4 cut(s) 198, 207, 341, 540
PspPPI RGGWCCY 1 cut(s) 198
PstNI CAGNNNCTG 1 cut(s) 518
PsuI RGATCY 1 cut(s) 695
RseI CAYNNNNRTG 1 cut(s) 683
SaqAI TTAA 4 cut(s) 260, 357, 597, 638
SatI GCNGC 3 cut(s) 27, 30, 227
Sau3AI GATC 1 cut(s) 695
Sau96I GGNCC 4 cut(s) 198, 207, 341, 540
SchI GAGTC 4 cut(s) 115, 530, 713, 1115
ScrFI CCNGG 5 cut(s) 87, 345, 629, 896, 897
SduI GDGCHC 2 cut(s) 54, 146
SfaNI GCATC 3 cut(s) 13, 121, 273
Sfr274I CTCGAG 1 cut(s) 1103
SfuI TTCGAA 1 cut(s) 492
SinI GGWCC 4 cut(s) 198, 207, 341, 540
SlaI CTCGAG 1 cut(s) 1103
SmaI CCCGGG 1 cut(s) 897
SmiMI CAYNNNNRTG 1 cut(s) 683
SmlI CTYRAG 1 cut(s) 1103
SmoI CTYRAG 1 cut(s) 1103
SspMI CTAG 3 cut(s) 158, 182, 708
StyD4I CCNGG 5 cut(s) 85, 343, 627, 894, 895
StyI CCWWGG 2 cut(s) 203, 781
TaqI TCGA 6 cut(s) 492, 572, 702, 902, 1104, 1109
TfiI GAWTC 2 cut(s) 272, 554
Tru1I TTAA 4 cut(s) 260, 357, 597, 638
Tru9I TTAA 4 cut(s) 260, 357, 597, 638
TscAI CASTG 1 cut(s) 85
TseFI GTSAC 3 cut(s) 815, 833, 920
TseI GCWGC 3 cut(s) 26, 29, 226
Tsp45I GTSAC 3 cut(s) 815, 833, 920
TspDTI ATGAA 2 cut(s) 537, 701
TspMI CCCGGG 1 cut(s) 895
TspRI CASTG 1 cut(s) 85
VpaK11BI GGWCC 4 cut(s) 198, 207, 341, 540
VspI ATTAAT 1 cut(s) 357
XapI RAATTY 4 cut(s) 291, 634, 913, 1047
XbaI TCTAGA 1 cut(s) 157
XcmI CCANNNNNNNNNTGG 2 cut(s) 653, 689
XhoI CTCGAG 1 cut(s) 1103
XmaI CCCGGG 1 cut(s) 895
XmnI GAANNNNTTC 1 cut(s) 293
XspI CTAG 3 cut(s) 158, 182, 708
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.