pycom08g16620

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0012630
Physical Location & Seq
Reverse (-)
16648679 .. 16649640
962 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 849 bp
ATGAAAATGATGTGTATATTTTATTTAATTATATATCACAATAAAACACTCTACACGTCTTGTGGCTTTTTGTTGTGTGGCCGTCGGCCAACCCAAAAAAAAAAAAAAAAAAAATCCCTCATCCCCCCCTCTCTCTCTCTCTCCCTCTCCCCCTCCCTCCTCTGCAGCAACAACAATATGACAACAAGCAGCAAAGGAAACACCACCACCACACCATCAGTAGTCCAAGTGGAAGCAGCAGGCAGCCGTGAGTGCTGCATGTGTGGGGATTTTGGCTTCTCCTATGAGCTCTTCCTTTGCAAGGTTTGCCAATTCAGATCTCAGCACAGGTACTGCAGCAATCTTTACCCAAACGCTGAGTCCTACAGAATCTGCAATTGGTGCCTCACCCAGAAGGAGGACACAAAGGAGAAGTCCCAAAACTCCTCAAATTCTTCCTCCTCATGCAAAAAGGAAACCCAAGATCAAGACCATCCAATTACCAAGATCACTAAGAAAAAGAATAATCCATCAAAAAATAGTAATAATTCATTTGGTGGGTGTCTGAGGGGCACTATGCAGCTGCAACCTAGTGGGCCCATCAAGAAACAGAGATCACTTGATCTTCATCATCTTCCTCGTCATTCATCAGCAGTATCTCCATCACCTTCACCATCTCCAAAATCTGCACCAGTTGTTACCAGAAAGAGGATCATCACAAAAGGGGAAAAGGAGGCTGAGAGGATCAAGAGGACAAGGTCAGAGGACATATCAAACACAAACAACACTAGTACTATTGGGATCACAAGGCAAGTCTTCAGGAACAAGGTCAGAAGATACAAGCTTCTAGATGAAGTTTCAAGTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

283

Amino Acids

31.78

Weight (kDa)

9.82

Isoelectric Point (pI)

55.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PHD_pln PF25054 86 - 128 2e-21 PHD-type zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 381
AclWI GGATC 3 cut(s) 698, 731, 788
AcoI YGGCCR 2 cut(s) 79, 86
AcsI RAATTY 1 cut(s) 430
AcuI CTGAAG 1 cut(s) 781
AfaI GTAC 2 cut(s) 332, 772
AfiI CCNNNNNNNGG 3 cut(s) 301, 397, 687
AflIII ACRYGT 1 cut(s) 54
AgsI TTSAA 1 cut(s) 840
AhlI ACTAGT 1 cut(s) 767
AjiI CACGTC 1 cut(s) 57
AjuI GAANNNNNNNTTGG 2 cut(s) 361, 393
AluBI AGCT 3 cut(s) 289, 562, 823
AluI AGCT 3 cut(s) 289, 562, 823
Alw21I GWGCWC 1 cut(s) 291
AlwI GGATC 3 cut(s) 698, 731, 788
AlwNI CAGNNNCTG 2 cut(s) 333, 372
AoxI GGCC 3 cut(s) 79, 86, 575
ApaI GGGCCC 1 cut(s) 579
ApeKI GCWGC 8 cut(s) 165, 189, 236, 243, 255, 336, 559, 562
ApoI RAATTY 1 cut(s) 430
ArsI GACNNNNNNTTYG 4 cut(s) 344, 376, 398, 430
AspS9I GGNCC 2 cut(s) 575, 576
AsuHPI GGTGA 3 cut(s) 379, 636, 642
BaeGI GKGCMC 2 cut(s) 554, 579
BanI GGYRCC 1 cut(s) 381
BanII GRGCYC 2 cut(s) 291, 579
BbsI GAAGAC 1 cut(s) 787
Bbv12I GWGCWC 1 cut(s) 291
BbvI GCAGC 8 cut(s) 177, 201, 242, 248, 255, 348, 549, 571
BccI CCATC 6 cut(s) 223, 480, 517, 587, 649, 661
BceAI ACGGC 2 cut(s) 66, 231
BcuI ACTAGT 1 cut(s) 767
BfaI CTAG 3 cut(s) 570, 768, 827
BfmI CTRYAG 3 cut(s) 163, 334, 364
BglII AGATCT 1 cut(s) 317
BisI GCNGC 8 cut(s) 166, 190, 237, 244, 256, 337, 560, 563
BlsI GCNGC 8 cut(s) 167, 191, 238, 245, 257, 338, 561, 564
BmcAI AGTACT 1 cut(s) 772
BmgBI CACGTC 1 cut(s) 57
BmgT120I GGNCC 2 cut(s) 575, 576
BmiI GGNNCC 2 cut(s) 383, 577
BpiI GAAGAC 1 cut(s) 787
BsaBI GATNNNNATC 1 cut(s) 606
Bsc4I CCNNNNNNNGG 3 cut(s) 301, 397, 687
Bse1I ACTGG 1 cut(s) 671
Bse8I GATNNNNATC 1 cut(s) 606
BseGI GGATG 2 cut(s) 120, 472
BseJI GATNNNNATC 1 cut(s) 606
BseLI CCNNNNNNNGG 3 cut(s) 301, 397, 687
BseMII CTCAG 4 cut(s) 335, 348, 536, 708
BseNI ACTGG 1 cut(s) 671
BseRI GAGGAG 3 cut(s) 149, 415, 430
BseSI GKGCMC 2 cut(s) 554, 579
BseXI GCAGC 8 cut(s) 177, 201, 242, 248, 255, 348, 549, 571
BsgI GTGCAG 1 cut(s) 651
BshFI GGCC 3 cut(s) 81, 88, 577
BshNI GGYRCC 1 cut(s) 381
BsiHKAI GWGCWC 1 cut(s) 291
BslFI GGGAC 1 cut(s) 400
BslI CCNNNNNNNGG 3 cut(s) 301, 397, 687
BsmFI GGGAC 1 cut(s) 400
BsnI GGCC 3 cut(s) 81, 88, 577
Bsp120I GGGCCC 1 cut(s) 575
Bsp1286I GDGCHC 3 cut(s) 291, 554, 579
Bsp143I GATC 8 cut(s) 317, 463, 486, 593, 601, 690, 723, 780
BspANI GGCC 3 cut(s) 81, 88, 577
BspCNI CTCAG 4 cut(s) 334, 349, 537, 709
BspLI GGNNCC 2 cut(s) 383, 577
BspMAI CTGCAG 2 cut(s) 167, 338
BspPI GGATC 3 cut(s) 698, 731, 788
BspQI GCTCTTC 1 cut(s) 296
BspT107I GGYRCC 1 cut(s) 381
BsrI ACTGG 1 cut(s) 671
BssMI GATC 8 cut(s) 317, 463, 486, 593, 601, 690, 723, 780
Bst6I CTCTTC 1 cut(s) 296
BstAPI GCANNNNNTGC 2 cut(s) 306, 381
BstC8I GCNNGC 1 cut(s) 241
BstDEI CTNAG 5 cut(s) 321, 357, 492, 545, 717
BstENI CCTNNNNNAGG 1 cut(s) 299
BstF5I GGATG 2 cut(s) 120, 472
BstKTI GATC 8 cut(s) 320, 466, 489, 596, 604, 693, 726, 783
BstMBI GATC 8 cut(s) 317, 463, 486, 593, 601, 690, 723, 780
BstMWI GCNNNNNNNGC 3 cut(s) 252, 306, 381
BstNSI RCATGY 1 cut(s) 262
BstSFI CTRYAG 3 cut(s) 163, 334, 364
BstSLI GKGCMC 2 cut(s) 554, 579
BstV1I GCAGC 8 cut(s) 177, 201, 242, 248, 255, 348, 549, 571
BstV2I GAAGAC 1 cut(s) 787
BstX2I RGATCY 1 cut(s) 317
BstYI RGATCY 1 cut(s) 317
BsuRI GGCC 3 cut(s) 81, 88, 577
BtrI CACGTC 1 cut(s) 57
BtsCI GGATG 2 cut(s) 120, 472
Cac8I GCNNGC 1 cut(s) 241
CaiI CAGNNNCTG 2 cut(s) 333, 372
Cfr13I GGNCC 2 cut(s) 575, 576
Csp6I GTAC 2 cut(s) 331, 771
CviAII CATG 2 cut(s) 259, 444
CviQI GTAC 2 cut(s) 331, 771
DdeI CTNAG 5 cut(s) 321, 357, 492, 545, 717
DpnI GATC 8 cut(s) 319, 465, 488, 595, 603, 692, 725, 782
DpnII GATC 8 cut(s) 317, 463, 486, 593, 601, 690, 723, 780
EaeI YGGCCR 2 cut(s) 79, 86
Eam1104I CTCTTC 1 cut(s) 296
EarI CTCTTC 1 cut(s) 296
Ecl136II GAGCTC 1 cut(s) 289
Eco24I GRGCYC 2 cut(s) 291, 579
Eco53kI GAGCTC 1 cut(s) 289
Eco57I CTGAAG 1 cut(s) 781
EcoICRI GAGCTC 1 cut(s) 289
EcoNI CCTNNNNNAGG 1 cut(s) 299
EcoT38I GRGCYC 2 cut(s) 291, 579
FaeI CATG 2 cut(s) 262, 447
FaiI YATR 9 cut(s) 17, 32, 34, 179, 260, 285, 445, 557, 749
FaqI GGGAC 1 cut(s) 400
FatI CATG 2 cut(s) 258, 443
Fnu4HI GCNGC 8 cut(s) 166, 190, 237, 244, 256, 337, 560, 563
FokI GGATG 2 cut(s) 107, 459
FriOI GRGCYC 2 cut(s) 291, 579
Fsp4HI GCNGC 8 cut(s) 166, 190, 237, 244, 256, 337, 560, 563
FspBI CTAG 3 cut(s) 570, 768, 827
GluI GCNGC 8 cut(s) 166, 190, 237, 244, 256, 337, 560, 563
HaeIII GGCC 3 cut(s) 81, 88, 577
Hin1II CATG 2 cut(s) 262, 447
HindIII AAGCTT 1 cut(s) 821
HinfI GANTC 2 cut(s) 359, 369
HphI GGTGA 3 cut(s) 379, 636, 642
Hpy188I TCNGA 4 cut(s) 317, 546, 742, 812
Hpy188III TCNNGA 5 cut(s) 467, 583, 727, 799, 827
Hpy99I CGWCG 1 cut(s) 87
HpyAV CCTTC 2 cut(s) 388, 657
HpyCH4IV ACGT 1 cut(s) 56
HpyCH4V TGCA 9 cut(s) 165, 258, 300, 336, 375, 447, 559, 565, 668
HpyF10VI GCNNNNNNNGC 3 cut(s) 252, 306, 381
HpyF3I CTNAG 5 cut(s) 321, 357, 492, 545, 717
HpySE526I ACGT 1 cut(s) 56
Hsp92II CATG 2 cut(s) 262, 447
Kzo9I GATC 8 cut(s) 317, 463, 486, 593, 601, 690, 723, 780
LguI GCTCTTC 1 cut(s) 296
LpnPI CCDG 6 cut(s) 225, 313, 404, 684, 694, 784
Lsp1109I GCAGC 8 cut(s) 177, 201, 242, 248, 255, 348, 549, 571
MaeI CTAG 3 cut(s) 570, 768, 827
MaeII ACGT 1 cut(s) 56
MaeIII GTNAC 1 cut(s) 676
MalI GATC 8 cut(s) 319, 465, 488, 595, 603, 692, 725, 782
MboI GATC 8 cut(s) 317, 463, 486, 593, 601, 690, 723, 780
MboII GAAGA 6 cut(s) 283, 426, 596, 605, 787, 825
MfeI CAATTG 1 cut(s) 376
MflI RGATCY 1 cut(s) 317
MhlI GDGCHC 3 cut(s) 291, 554, 579
MluCI AATT 6 cut(s) 27, 311, 376, 430, 477, 526
MlyI GAGTC 1 cut(s) 368
MseI TTAA 1 cut(s) 26
MspA1I CMGCKG 1 cut(s) 562
MunI CAATTG 1 cut(s) 376
MwoI GCNNNNNNNGC 3 cut(s) 252, 306, 381
NdeII GATC 8 cut(s) 317, 463, 486, 593, 601, 690, 723, 780
NlaIII CATG 2 cut(s) 262, 447
NlaIV GGNNCC 2 cut(s) 383, 577
NspI RCATGY 1 cut(s) 262
PciSI GCTCTTC 1 cut(s) 296
PfeI GAWTC 1 cut(s) 369
PflFI GACNNNGTC 1 cut(s) 736
PkrI GCNGC 8 cut(s) 167, 191, 238, 245, 257, 338, 561, 564
PleI GAGTC 1 cut(s) 367
PpsI GAGTC 1 cut(s) 367
Psp124BI GAGCTC 1 cut(s) 291
PspN4I GGNNCC 2 cut(s) 383, 577
PspOMI GGGCCC 1 cut(s) 575
PspPI GGNCC 2 cut(s) 575, 576
PstI CTGCAG 2 cut(s) 167, 338
PstNI CAGNNNCTG 2 cut(s) 333, 372
PsuI RGATCY 1 cut(s) 317
PsyI GACNNNGTC 1 cut(s) 736
PvuII CAGCTG 1 cut(s) 562
RsaI GTAC 2 cut(s) 332, 772
RsaNI GTAC 2 cut(s) 331, 771
SacI GAGCTC 1 cut(s) 291
SapI GCTCTTC 1 cut(s) 296
SaqAI TTAA 1 cut(s) 26
SatI GCNGC 8 cut(s) 166, 190, 237, 244, 256, 337, 560, 563
Sau3AI GATC 8 cut(s) 317, 463, 486, 593, 601, 690, 723, 780
Sau96I GGNCC 2 cut(s) 575, 576
ScaI AGTACT 1 cut(s) 772
SchI GAGTC 1 cut(s) 368
SduI GDGCHC 3 cut(s) 291, 554, 579
SfcI CTRYAG 3 cut(s) 163, 334, 364
SpeI ACTAGT 1 cut(s) 767
Sse9I AATT 6 cut(s) 27, 311, 376, 430, 477, 526
SspMI CTAG 3 cut(s) 570, 768, 827
SstI GAGCTC 1 cut(s) 291
TaiI ACGT 1 cut(s) 59
TasI AATT 6 cut(s) 27, 311, 376, 430, 477, 526
TatI WGTACW 1 cut(s) 770
TfiI GAWTC 1 cut(s) 369
Tru1I TTAA 1 cut(s) 26
Tru9I TTAA 1 cut(s) 26
TseI GCWGC 8 cut(s) 165, 189, 236, 243, 255, 336, 559, 562
TspDTI ATGAA 5 cut(s) 17, 519, 596, 615, 846
Tth111I GACNNNGTC 1 cut(s) 736
XagI CCTNNNNNAGG 1 cut(s) 299
XapI RAATTY 1 cut(s) 430
XbaI TCTAGA 1 cut(s) 826
XceI RCATGY 1 cut(s) 262
XspI CTAG 3 cut(s) 570, 768, 827
ZrmI AGTACT 1 cut(s) 772
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.