pycom08g19190

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0014749
Physical Location & Seq
Reverse (-)
19217837 .. 19220848
3012 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 714 bp
ATGGTGAAGTCATTCAAAACACAGTGCCTGTTTGCGTTACTAATTAGCAACCAATTATATATCATGAACATGAAAAGCAGCAGCAGTACTAAGGAGAAGATGAAGAAGAAGATGATGATGAGGGGGTTCATGTGTCAGTCTCAAGCAGCAACTGCGGTGTGCATGTCTGATGCTCGCTCAGTTATCGTGCCACCGCCGACAAGACCAGCTGCAGCTACTAGTGTCCGCCACAATCACAGTGCTGGCCATGTGAATTACATGATGATGAGAAGATCAACTAGTACTAATAATGGTGGCGGCGGTGGTCACTACTACTACTCCAGGCTCCTCGATACTACTCCTCCAGCTCATGGCCATGAGACGTCCCACTACCACAGTCGTAGACGAAGCGCTGGTAGTACCAGCAGTATTCTTAACCACCAAGATCAGCGGCCAATGTCAAAGTCGACACGGAAACTACCATCATCTGATCCAGATTTACTACCACCGTCGTCATCCGCCGACAATGTCTTCCAGGTGGTTGTTATGAGAGTTTCTATTCATTGCCATGGATGTGCTGGTAAATTGAAGAAACATCTCTCTAAAATGGAAGGGGTTACATCATTCAGTATAGACCTAGAGACAAAGATGGTAACTGTTAGGGGACACGTTTCACCAGTTGGAGTCGTGGAGAGCATTTCAAAAGTGAAGAAGGCAGAGTTATTAACCGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

238

Amino Acids

25.98

Weight (kDa)

10.09

Isoelectric Point (pI)

60.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 176 - 230 4e-12 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014749)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g30890
malus_domestica MD08G1221300.v1.1 MD15G1412000.v1.1
prunus_persica Prupe.1G558400_v2.0.a1 Prupe.1G558400_v2.0.a1
pyrus_communis pycom08g19190 pycom15g36680
rosa_chinensis RchiOBHm_Chr7g0226171
rosa_laevigata RLG00000001761
rosa_multiflora Rmu_sc0001350.1_g000012
rosa_roxburghii Rroxscaffold_3G00233160
rosa_rugosa Rorug07G0235200
rosa_samantha Rh7AG382200 Rh7BG369200 Rh7CG401700 Rh7DG380600
rosa_wichuraiana Rw7G032000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 365
AccB7I CCANNNNNTGG 1 cut(s) 350
AccI GTMKAC 2 cut(s) 382, 446
AciI CCGC 7 cut(s) 155, 194, 226, 297, 300, 430, 498
AclWI GGATC 1 cut(s) 464
AcoI YGGCCR 3 cut(s) 244, 352, 431
AcyI GRCGYC 1 cut(s) 362
AfaI GTAC 3 cut(s) 88, 283, 400
AfeI AGCGCT 1 cut(s) 391
AfiI CCNNNNNNNGG 1 cut(s) 350
AflIII ACRYGT 1 cut(s) 646
AgeI ACCGGT 1 cut(s) 707
AgsI TTSAA 3 cut(s) 16, 568, 681
AhlI ACTAGT 2 cut(s) 218, 278
AjnI CCWGG 2 cut(s) 320, 513
AluBI AGCT 3 cut(s) 209, 215, 347
AluI AGCT 3 cut(s) 209, 215, 347
Alw26I GTCTC 3 cut(s) 144, 353, 614
AlwI GGATC 1 cut(s) 464
AlwNI CAGNNNCTG 2 cut(s) 28, 152
Aor51HI AGCGCT 1 cut(s) 391
AoxI GGCC 3 cut(s) 244, 352, 431
ApeKI GCWGC 5 cut(s) 78, 81, 146, 209, 212
AsiGI ACCGGT 1 cut(s) 707
Asp700I GAANNNNTTC 1 cut(s) 11
AspLEI GCGC 1 cut(s) 392
AsuHPI GGTGA 2 cut(s) 16, 645
BalI TGGCCA 2 cut(s) 246, 354
BbsI GAAGAC 1 cut(s) 502
BbvI GCAGC 5 cut(s) 90, 93, 158, 196, 224
BccI CCATC 2 cut(s) 469, 622
BciT130I CCWGG 2 cut(s) 322, 515
BcoDI GTCTC 3 cut(s) 144, 353, 614
BcuI ACTAGT 2 cut(s) 218, 278
BfaI CTAG 3 cut(s) 219, 279, 617
BfmI CTRYAG 1 cut(s) 210
BfoI RGCGCY 1 cut(s) 393
BisI GCNGC 7 cut(s) 79, 82, 147, 210, 213, 298, 431
BlsI GCNGC 7 cut(s) 80, 83, 148, 211, 214, 299, 432
BmcAI AGTACT 2 cut(s) 88, 283
Bme1390I CCNGG 2 cut(s) 322, 515
BmiI GGNNCC 1 cut(s) 326
BmrFI CCNGG 2 cut(s) 322, 515
BmsI GCATC 1 cut(s) 160
BpiI GAAGAC 1 cut(s) 502
BpmI CTGGAG 2 cut(s) 304, 327
BpuEI CTTGAG 1 cut(s) 126
BsaHI GRCGYC 1 cut(s) 362
BsaJI CCNNGG 1 cut(s) 547
BsaWI WCCGGW 1 cut(s) 707
BsaXI ACNNNNNCTCC 4 cut(s) 302, 325, 332, 355
Bsc4I CCNNNNNNNGG 1 cut(s) 350
Bse118I RCCGGY 1 cut(s) 707
Bse1I ACTGG 1 cut(s) 656
Bse3DI GCAATG 1 cut(s) 541
BseBI CCWGG 2 cut(s) 322, 515
BseDI CCNNGG 1 cut(s) 547
BseGI GGATG 2 cut(s) 494, 557
BseLI CCNNNNNNNGG 1 cut(s) 350
BseMI GCAATG 1 cut(s) 541
BseMII CTCAG 1 cut(s) 192
BseNI ACTGG 1 cut(s) 656
BseRI GAGGAG 2 cut(s) 317, 330
BseXI GCAGC 5 cut(s) 90, 93, 158, 196, 224
BshFI GGCC 3 cut(s) 246, 354, 433
BshTI ACCGGT 1 cut(s) 707
BsiSI CCGG 1 cut(s) 708
BslFI GGGAC 2 cut(s) 349, 657
BslI CCNNNNNNNGG 1 cut(s) 350
BsmAI GTCTC 3 cut(s) 144, 353, 614
BsmBI CGTCTC 1 cut(s) 353
BsmFI GGGAC 2 cut(s) 349, 657
BsnI GGCC 3 cut(s) 246, 354, 433
Bsp143I GATC 3 cut(s) 272, 424, 469
Bsp19I CCATGG 1 cut(s) 547
BspACI CCGC 7 cut(s) 155, 194, 226, 297, 300, 430, 498
BspANI GGCC 3 cut(s) 246, 354, 433
BspCNI CTCAG 1 cut(s) 191
BspHI TCATGA 1 cut(s) 63
BspLI GGNNCC 1 cut(s) 326
BspMAI CTGCAG 1 cut(s) 214
BspPI GGATC 1 cut(s) 464
BsrDI GCAATG 1 cut(s) 541
BsrFI RCCGGY 1 cut(s) 707
BsrI ACTGG 1 cut(s) 656
BssAI RCCGGY 1 cut(s) 707
BssECI CCNNGG 1 cut(s) 547
BssMI GATC 3 cut(s) 272, 424, 469
BssNI GRCGYC 1 cut(s) 362
BssT1I CCWWGG 1 cut(s) 547
Bst2UI CCWGG 2 cut(s) 322, 515
Bst4CI ACNGT 5 cut(s) 24, 239, 377, 489, 637
BstACI GRCGYC 1 cut(s) 362
BstAPI GCANNNNNTGC 1 cut(s) 152
BstC8I GCNNGC 2 cut(s) 175, 244
BstDEI CTNAG 2 cut(s) 90, 178
BstDSI CCRYGG 1 cut(s) 547
BstF5I GGATG 2 cut(s) 494, 557
BstH2I RGCGCY 1 cut(s) 393
BstHHI GCGC 1 cut(s) 392
BstKTI GATC 3 cut(s) 275, 427, 472
BstMAI GTCTC 3 cut(s) 144, 353, 614
BstMBI GATC 3 cut(s) 272, 424, 469
BstMWI GCNNNNNNNGC 1 cut(s) 152
BstNI CCWGG 2 cut(s) 322, 515
BstNSI RCATGY 1 cut(s) 166
BstSCI CCNGG 2 cut(s) 320, 513
BstSFI CTRYAG 1 cut(s) 210
BstV1I GCAGC 5 cut(s) 90, 93, 158, 196, 224
BstV2I GAAGAC 1 cut(s) 502
BsuRI GGCC 3 cut(s) 246, 354, 433
BtgI CCRYGG 1 cut(s) 547
BtsCI GGATG 2 cut(s) 494, 557
BtsIMutI CAGTG 2 cut(s) 29, 244
Cac8I GCNNGC 2 cut(s) 175, 244
CaiI CAGNNNCTG 2 cut(s) 28, 152
CciI TCATGA 1 cut(s) 63
CfoI GCGC 1 cut(s) 392
Cfr10I RCCGGY 1 cut(s) 707
Csp6I GTAC 3 cut(s) 87, 282, 399
CspAI ACCGGT 1 cut(s) 707
CviAII CATG 9 cut(s) 64, 70, 130, 163, 248, 259, 350, 356, 548
CviJI RGCY 7 cut(s) 209, 215, 246, 325, 347, 354, 433
CviKI_1 RGCY 7 cut(s) 209, 215, 246, 325, 347, 354, 433
CviQI GTAC 3 cut(s) 87, 282, 399
DdeI CTNAG 2 cut(s) 90, 178
DpnI GATC 3 cut(s) 274, 426, 471
DpnII GATC 3 cut(s) 272, 424, 469
EaeI YGGCCR 3 cut(s) 244, 352, 431
EciI GGCGGA 2 cut(s) 215, 487
Eco130I CCWWGG 1 cut(s) 547
Eco47III AGCGCT 1 cut(s) 391
EcoRII CCWGG 2 cut(s) 320, 513
EcoT14I CCWWGG 1 cut(s) 547
ErhI CCWWGG 1 cut(s) 547
Esp3I CGTCTC 1 cut(s) 353
FaeI CATG 9 cut(s) 67, 73, 133, 166, 251, 262, 353, 359, 551
FaqI GGGAC 2 cut(s) 349, 657
FatI CATG 9 cut(s) 63, 69, 129, 162, 247, 258, 349, 355, 547
FblI GTMKAC 2 cut(s) 382, 446
Fnu4HI GCNGC 7 cut(s) 79, 82, 147, 210, 213, 298, 431
FokI GGATG 2 cut(s) 481, 564
Fsp4HI GCNGC 7 cut(s) 79, 82, 147, 210, 213, 298, 431
FspBI CTAG 3 cut(s) 219, 279, 617
GlaI GCGC 1 cut(s) 391
GluI GCNGC 7 cut(s) 79, 82, 147, 210, 213, 298, 431
GsuI CTGGAG 2 cut(s) 304, 327
HaeII RGCGCY 1 cut(s) 393
HaeIII GGCC 3 cut(s) 246, 354, 433
HapII CCGG 1 cut(s) 708
HhaI GCGC 1 cut(s) 392
Hin1I GRCGYC 1 cut(s) 362
Hin1II CATG 9 cut(s) 67, 73, 133, 166, 251, 262, 353, 359, 551
Hin6I GCGC 1 cut(s) 390
HinP1I GCGC 1 cut(s) 390
HincII GTYRAC 1 cut(s) 447
HindII GTYRAC 1 cut(s) 447
HinfI GANTC 1 cut(s) 663
HpaII CCGG 1 cut(s) 708
HphI GGTGA 2 cut(s) 16, 645
Hpy166II GTNNAC 2 cut(s) 383, 447
Hpy188I TCNGA 2 cut(s) 169, 469
Hpy188III TCNNGA 2 cut(s) 64, 473
Hpy8I GTNNAC 2 cut(s) 383, 447
Hpy99I CGWCG 1 cut(s) 493
HpyAV CCTTC 2 cut(s) 584, 685
HpyCH4III ACNGT 5 cut(s) 24, 239, 377, 489, 637
HpyCH4IV ACGT 2 cut(s) 362, 648
HpyCH4V TGCA 2 cut(s) 162, 212
HpyF10VI GCNNNNNNNGC 1 cut(s) 152
HpyF3I CTNAG 2 cut(s) 90, 178
HpySE526I ACGT 2 cut(s) 362, 648
Hsp92I GRCGYC 1 cut(s) 362
Hsp92II CATG 9 cut(s) 67, 73, 133, 166, 251, 262, 353, 359, 551
HspAI GCGC 1 cut(s) 390
Kzo9I GATC 3 cut(s) 272, 424, 469
LmnI GCTCC 1 cut(s) 330
Lsp1109I GCAGC 5 cut(s) 90, 93, 158, 196, 224
LweI GCATC 1 cut(s) 160
MaeI CTAG 3 cut(s) 219, 279, 617
MaeII ACGT 2 cut(s) 362, 648
MaeIII GTNAC 4 cut(s) 36, 305, 595, 631
MalI GATC 3 cut(s) 274, 426, 471
MboI GATC 3 cut(s) 272, 424, 469
MboII GAAGA 8 cut(s) 109, 115, 118, 121, 282, 502, 580, 700
MlsI TGGCCA 2 cut(s) 246, 354
MluCI AATT 4 cut(s) 42, 53, 253, 563
MluNI TGGCCA 2 cut(s) 246, 354
MlyI GAGTC 1 cut(s) 672
MmeI TCCRAC 1 cut(s) 640
MnlI CCTC 3 cut(s) 114, 338, 351
Mox20I TGGCCA 2 cut(s) 246, 354
MroXI GAANNNNTTC 1 cut(s) 11
MscI TGGCCA 2 cut(s) 246, 354
MseI TTAA 2 cut(s) 414, 704
MslI CAYNNNNRTG 5 cut(s) 68, 263, 354, 546, 552
Msp20I TGGCCA 2 cut(s) 246, 354
MspA1I CMGCKG 2 cut(s) 209, 430
MspI CCGG 1 cut(s) 708
MspR9I CCNGG 2 cut(s) 322, 515
MvaI CCWGG 2 cut(s) 322, 515
MwoI GCNNNNNNNGC 1 cut(s) 152
NcoI CCATGG 1 cut(s) 547
NdeII GATC 3 cut(s) 272, 424, 469
NlaIII CATG 9 cut(s) 67, 73, 133, 166, 251, 262, 353, 359, 551
NlaIV GGNNCC 1 cut(s) 326
NmuCI GTSAC 1 cut(s) 305
NspI RCATGY 1 cut(s) 166
PagI TCATGA 1 cut(s) 63
PdmI GAANNNNTTC 1 cut(s) 11
PflFI GACNNNGTC 1 cut(s) 506
PflMI CCANNNNNTGG 1 cut(s) 350
PinAI ACCGGT 1 cut(s) 707
PkrI GCNGC 7 cut(s) 80, 83, 148, 211, 214, 299, 432
PleI GAGTC 1 cut(s) 671
PpsI GAGTC 1 cut(s) 671
Psp6I CCWGG 2 cut(s) 320, 513
PspGI CCWGG 2 cut(s) 320, 513
PspN4I GGNNCC 1 cut(s) 326
PstI CTGCAG 1 cut(s) 214
PstNI CAGNNNCTG 2 cut(s) 28, 152
PsyI GACNNNGTC 1 cut(s) 506
PvuII CAGCTG 1 cut(s) 209
RsaI GTAC 3 cut(s) 88, 283, 400
RsaNI GTAC 3 cut(s) 87, 282, 399
RseI CAYNNNNRTG 5 cut(s) 68, 263, 354, 546, 552
SalI GTCGAC 1 cut(s) 445
SaqAI TTAA 2 cut(s) 414, 704
SatI GCNGC 7 cut(s) 79, 82, 147, 210, 213, 298, 431
Sau3AI GATC 3 cut(s) 272, 424, 469
ScaI AGTACT 2 cut(s) 88, 283
SchI GAGTC 1 cut(s) 672
ScrFI CCNGG 2 cut(s) 322, 515
SetI ASST 7 cut(s) 211, 217, 349, 365, 519, 618, 651
SfaNI GCATC 1 cut(s) 160
SfcI CTRYAG 1 cut(s) 210
SmiMI CAYNNNNRTG 5 cut(s) 68, 263, 354, 546, 552
SmlI CTYRAG 1 cut(s) 141
SmoI CTYRAG 1 cut(s) 141
SpeI ACTAGT 2 cut(s) 218, 278
Sse9I AATT 4 cut(s) 42, 53, 253, 563
SsiI CCGC 7 cut(s) 155, 194, 226, 297, 300, 430, 498
SspMI CTAG 3 cut(s) 219, 279, 617
StyD4I CCNGG 2 cut(s) 320, 513
StyI CCWWGG 1 cut(s) 547
TaaI ACNGT 5 cut(s) 24, 239, 377, 489, 637
TaiI ACGT 2 cut(s) 365, 651
TaqI TCGA 2 cut(s) 330, 446
TasI AATT 4 cut(s) 42, 53, 253, 563
TatI WGTACW 2 cut(s) 86, 281
TauI GCSGC 2 cut(s) 300, 433
Tru1I TTAA 2 cut(s) 414, 704
Tru9I TTAA 2 cut(s) 414, 704
TscAI CASTG 2 cut(s) 29, 244
TseFI GTSAC 1 cut(s) 305
TseI GCWGC 5 cut(s) 78, 81, 146, 209, 212
Tsp45I GTSAC 1 cut(s) 305
TspDTI ATGAA 5 cut(s) 80, 86, 116, 118, 530
TspGWI ACGGA 1 cut(s) 466
TspRI CASTG 2 cut(s) 29, 244
Tth111I GACNNNGTC 1 cut(s) 506
Van91I CCANNNNNTGG 1 cut(s) 350
XceI RCATGY 1 cut(s) 166
XcmI CCANNNNNNNNNTGG 1 cut(s) 554
XmiI GTMKAC 2 cut(s) 382, 446
XmnI GAANNNNTTC 1 cut(s) 11
XspI CTAG 3 cut(s) 219, 279, 617
ZraI GACGTC 1 cut(s) 363
ZrmI AGTACT 2 cut(s) 88, 283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.