pycom08g21320

Protein of unknown function (DUF707)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0004588
Physical Location & Seq
Forward (+)
20824497 .. 20829932
5436 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1188 bp
ATGGGATCAAATGCCGCTGCGCTGTCTGACCCGAAGAATAGGTCATTCTACTGCAGTCTCTTCATTCTAGCTTCTTTGATCTGCGGGGCTTACTTCATCGGTGGTGCAACCATTGCTCACGAGTACAAGGAAAAATTAACAAGATGGAAAGTGGTCTACAATAGGCAGAATACAAATTTTCATACGTGCAAGAATCGTTGCCAACCTTCAGGGAGTGAGGCATTACCGGAAGGAATTATTGCCAAAACATCTGACTTGGAACCGCTGCCTCTATGGGGTTCATCTGTGAAAAATGAAAACTCAAAACCTTCTAAGAGCTTGCTGGCAATTGCAGTTGGAATAAGGCAGAAAGAAATAGTGGACAAAATTGTCAAAAAGTTTCTATCAAGTGATTTTGTTGTTATGCTTTTTCATTATGATGGTGCGTTGGATAAGTGGAGGGATTTACCTTGGAGTGATCGTGCCATACACGTGTCTGTGATGAATCAAACAAAATGGTGGTTCGCCAAACGTTTCCTGCATCCAGATATAGTTTCCGAGTACAAATATATTTTTCTTTGGGATGAGGACCTTGGAGTTGAGAATTTTAACCCAAAACGATATGTATCCATTATTCAGGAAGAGGGACTTGAAATATCACAGCCAGCACTTGATCCTGACAAGTCAGAGGTGTATCATCCAATCACTGCACGTGTGAAGAAATCAAAAGTGCACAGAAGGTTTCATAAATATAAAGGCAGTGCAAGGTGTGACGACCATAGTTCTGCTCCTCCTTGTGCTGGTTGGGTGGAAATGATGGCACCTGTATTCTCAAGATCTGCATGGCAATGTGTATGGTACATGATCCAGAATGATCTGGTCCATGCATGGGGCCTGGATGTGCAGCTTGGTTATTGTGCACAAGGAGATCGAACACAAAACGTTGGTGTGGTTGACTCAGAGTATATAGTTCATCTTGGTCTTCCTACGCTCGGTGTCTCAGATGGCAATAAGGTGACCAATGGCCCGGATCCTTCTCAGAAAGGGGACTCGAAAGCATTGGCACCATCTTCCTCGGATAAAGTCAATGATCGATCTAAAGTTAGGATGCAGTCCTTTATTGACATGCAGATCTTTAAGGAAAGATGGAGTGCTGCAATAAGGGAGGATAAATGTTGGGTTGACCCGTATCAACTATCAACAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF707

Protein Analysis

396

Amino Acids

44.82

Weight (kDa)

8.57

Isoelectric Point (pI)

33.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF707 PF05212 67 - 381 8.8e-145 Protein of unknown function (DUF707)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 368
AccB1I GGYRCC 2 cut(s) 799, 1042
AccI GTMKAC 1 cut(s) 156
AciI CCGC 3 cut(s) 15, 84, 263
AclI AACGTT 2 cut(s) 511, 921
AclWI GGATC 5 cut(s) 13, 647, 838, 1004, 1017
AcsI RAATTY 2 cut(s) 175, 583
AcuI CTGAAG 1 cut(s) 192
AcvI CACGTG 2 cut(s) 472, 692
AfaI GTAC 3 cut(s) 125, 542, 839
AfiI CCNNNNNNNGG 4 cut(s) 275, 779, 868, 971
AflIII ACRYGT 3 cut(s) 469, 471, 691
AgsI TTSAA 1 cut(s) 632
AjnI CCWGG 1 cut(s) 873
AluBI AGCT 3 cut(s) 71, 318, 886
AluI AGCT 3 cut(s) 71, 318, 886
Alw21I GWGCWC 2 cut(s) 714, 901
Alw26I GTCTC 2 cut(s) 62, 982
Alw44I GTGCAC 2 cut(s) 710, 897
AlwI GGATC 5 cut(s) 13, 647, 838, 1004, 1017
AoxI GGCC 2 cut(s) 871, 1003
ApaLI GTGCAC 2 cut(s) 710, 897
ApeKI GCWGC 4 cut(s) 17, 265, 883, 1133
ApoI RAATTY 2 cut(s) 175, 583
ArsI GACNNNNNNTTYG 2 cut(s) 26, 58
AspLEI GCGC 1 cut(s) 22
AspS9I GGNCC 4 cut(s) 568, 859, 871, 1004
AsuC2I CCSGG 1 cut(s) 1007
AsuHPI GGTGA 1 cut(s) 1006
AvaII GGWCC 2 cut(s) 568, 859
BaeGI GKGCMC 2 cut(s) 714, 901
BamHI GGATCC 1 cut(s) 1009
BanI GGYRCC 2 cut(s) 799, 1042
BauI CACGAG 1 cut(s) 119
BbrPI CACGTG 2 cut(s) 472, 692
BbsI GAAGAC 1 cut(s) 953
Bbv12I GWGCWC 2 cut(s) 714, 901
BbvI GCAGC 4 cut(s) 4, 252, 895, 1120
BccI CCATC 6 cut(s) 138, 413, 790, 977, 1054, 1119
BciT130I CCWGG 1 cut(s) 875
BciVI GTATCC 1 cut(s) 616
BcnI CCSGG 1 cut(s) 1007
BcoDI GTCTC 2 cut(s) 62, 982
BfaI CTAG 1 cut(s) 68
BfmI CTRYAG 1 cut(s) 52
BfuI GTATCC 1 cut(s) 616
BglII AGATCT 2 cut(s) 815, 1110
BisI GCNGC 5 cut(s) 15, 18, 266, 884, 1134
BlsI GCNGC 5 cut(s) 16, 19, 267, 885, 1135
Bme1390I CCNGG 2 cut(s) 875, 1007
Bme18I GGWCC 2 cut(s) 568, 859
BmgT120I GGNCC 4 cut(s) 568, 859, 871, 1004
BmiI GGNNCC 5 cut(s) 261, 801, 872, 1011, 1044
BmrFI CCNGG 2 cut(s) 875, 1007
BmsI GCATC 2 cut(s) 529, 1077
BpiI GAAGAC 1 cut(s) 953
BpuEI CTTGAG 1 cut(s) 796
BpuMI CCSGG 1 cut(s) 1007
Bsa29I ATCGAT 1 cut(s) 1072
BsaAI YACGTR 3 cut(s) 186, 472, 692
BsaBI GATNNNNATC 1 cut(s) 604
BsaJI CCNNGG 3 cut(s) 449, 571, 1053
BsaWI WCCGGW 1 cut(s) 226
BsaXI ACNNNNNCTCC 2 cut(s) 445, 475
Bsc4I CCNNNNNNNGG 4 cut(s) 275, 779, 868, 971
Bse3DI GCAATG 2 cut(s) 111, 833
Bse8I GATNNNNATC 1 cut(s) 604
BseBI CCWGG 1 cut(s) 875
BseCI ATCGAT 1 cut(s) 1072
BseDI CCNNGG 3 cut(s) 449, 571, 1053
BseGI GGATG 5 cut(s) 520, 568, 676, 883, 1092
BseJI GATNNNNATC 1 cut(s) 604
BseLI CCNNNNNNNGG 4 cut(s) 275, 779, 868, 971
BseMI GCAATG 2 cut(s) 111, 833
BseMII CTCAG 3 cut(s) 951, 993, 1031
BseRI GAGGAG 1 cut(s) 759
BseSI GKGCMC 2 cut(s) 714, 901
BseXI GCAGC 4 cut(s) 4, 252, 895, 1120
BsgI GTGCAG 2 cut(s) 672, 902
BshFI GGCC 2 cut(s) 873, 1005
BshNI GGYRCC 2 cut(s) 799, 1042
BshVI ATCGAT 1 cut(s) 1072
BsiHKAI GWGCWC 2 cut(s) 714, 901
BsiSI CCGG 2 cut(s) 227, 1007
BslFI GGGAC 2 cut(s) 639, 1040
BslI CCNNNNNNNGG 4 cut(s) 275, 779, 868, 971
BsmAI GTCTC 2 cut(s) 62, 982
BsmFI GGGAC 2 cut(s) 639, 1040
BsnI GGCC 2 cut(s) 873, 1005
Bsp1286I GDGCHC 2 cut(s) 714, 901
BspACI CCGC 3 cut(s) 15, 84, 263
BspANI GGCC 2 cut(s) 873, 1005
BspCNI CTCAG 3 cut(s) 950, 992, 1030
BspDI ATCGAT 1 cut(s) 1072
BspLI GGNNCC 5 cut(s) 261, 801, 872, 1011, 1044
BspMAI CTGCAG 1 cut(s) 56
BspPI GGATC 5 cut(s) 13, 647, 838, 1004, 1017
BspT107I GGYRCC 2 cut(s) 799, 1042
BsrDI GCAATG 2 cut(s) 111, 833
BssECI CCNNGG 3 cut(s) 449, 571, 1053
BssSI CACGAG 1 cut(s) 119
BssT1I CCWWGG 2 cut(s) 449, 571
Bst2BI CACGAG 1 cut(s) 119
Bst2UI CCWGG 1 cut(s) 875
Bst6I CTCTTC 2 cut(s) 65, 615
BstAPI GCANNNNNTGC 1 cut(s) 113
BstBAI YACGTR 3 cut(s) 186, 472, 692
BstC8I GCNNGC 3 cut(s) 320, 324, 645
BstDEI CTNAG 4 cut(s) 312, 937, 979, 1017
BstEII GGTNACC 1 cut(s) 994
BstF5I GGATG 5 cut(s) 520, 568, 676, 883, 1092
BstHHI GCGC 1 cut(s) 22
BstMAI GTCTC 2 cut(s) 62, 982
BstMWI GCNNNNNNNGC 1 cut(s) 113
BstNI CCWGG 1 cut(s) 875
BstNSI RCATGY 1 cut(s) 1108
BstPI GGTNACC 1 cut(s) 994
BstSCI CCNGG 2 cut(s) 873, 1005
BstSFI CTRYAG 1 cut(s) 52
BstSLI GKGCMC 2 cut(s) 714, 901
BstV1I GCAGC 4 cut(s) 4, 252, 895, 1120
BstV2I GAAGAC 1 cut(s) 953
BstX2I RGATCY 3 cut(s) 815, 1009, 1110
BstYI RGATCY 3 cut(s) 815, 1009, 1110
Bsu15I ATCGAT 1 cut(s) 1072
BsuI GTATCC 1 cut(s) 616
BsuRI GGCC 2 cut(s) 873, 1005
BsuTUI ATCGAT 1 cut(s) 1072
BtsCI GGATG 5 cut(s) 520, 568, 676, 883, 1092
BtsI GCAGTG 2 cut(s) 684, 745
BtsIMutI CAGTG 2 cut(s) 684, 745
Cac8I GCNNGC 3 cut(s) 320, 324, 645
CfoI GCGC 1 cut(s) 22
Cfr13I GGNCC 4 cut(s) 568, 859, 871, 1004
ClaI ATCGAT 1 cut(s) 1072
Csp6I GTAC 3 cut(s) 124, 541, 838
CviAII CATG 5 cut(s) 822, 841, 863, 867, 1105
CviJI RGCY 7 cut(s) 71, 89, 318, 643, 873, 886, 1005
CviKI_1 RGCY 7 cut(s) 71, 89, 318, 643, 873, 886, 1005
CviQI GTAC 3 cut(s) 124, 541, 838
DdeI CTNAG 4 cut(s) 312, 937, 979, 1017
DrdI GACNNNNNNGTC 1 cut(s) 368
DseDI GACNNNNNNGTC 1 cut(s) 368
Eam1104I CTCTTC 2 cut(s) 65, 615
EarI CTCTTC 2 cut(s) 65, 615
Eco130I CCWWGG 2 cut(s) 449, 571
Eco47I GGWCC 2 cut(s) 568, 859
Eco57I CTGAAG 1 cut(s) 192
Eco72I CACGTG 2 cut(s) 472, 692
Eco91I GGTNACC 1 cut(s) 994
EcoO109I RGGNCCY 2 cut(s) 568, 871
EcoO65I GGTNACC 1 cut(s) 994
EcoRII CCWGG 1 cut(s) 873
EcoT14I CCWWGG 2 cut(s) 449, 571
EcoT22I ATGCAT 1 cut(s) 868
ErhI CCWWGG 2 cut(s) 449, 571
FaeI CATG 5 cut(s) 825, 844, 866, 870, 1108
FalI AAGNNNNNCTT 2 cut(s) 612, 644
FaqI GGGAC 2 cut(s) 639, 1040
FatI CATG 5 cut(s) 821, 840, 862, 866, 1104
FauI CCCGC 1 cut(s) 77
FblI GTMKAC 1 cut(s) 156
Fnu4HI GCNGC 5 cut(s) 15, 18, 266, 884, 1134
FokI GGATG 5 cut(s) 507, 575, 663, 890, 1099
Fsp4HI GCNGC 5 cut(s) 15, 18, 266, 884, 1134
FspBI CTAG 1 cut(s) 68
GlaI GCGC 1 cut(s) 21
GluI GCNGC 5 cut(s) 15, 18, 266, 884, 1134
HaeIII GGCC 2 cut(s) 873, 1005
HapII CCGG 2 cut(s) 227, 1007
HhaI GCGC 1 cut(s) 22
Hin1II CATG 5 cut(s) 825, 844, 866, 870, 1108
Hin6I GCGC 1 cut(s) 20
HinP1I GCGC 1 cut(s) 20
HincII GTYRAC 2 cut(s) 934, 1162
HindII GTYRAC 2 cut(s) 934, 1162
HinfI GANTC 4 cut(s) 193, 484, 935, 1028
HpaII CCGG 2 cut(s) 227, 1007
HphI GGTGA 1 cut(s) 1006
Hpy166II GTNNAC 6 cut(s) 157, 361, 712, 899, 934, 1162
Hpy188I TCNGA 8 cut(s) 28, 253, 538, 667, 940, 982, 1020, 1057
Hpy188III TCNNGA 6 cut(s) 119, 524, 617, 656, 813, 847
Hpy8I GTNNAC 6 cut(s) 157, 361, 712, 899, 934, 1162
HpyAV CCTTC 5 cut(s) 216, 224, 318, 711, 1023
HpyCH4IV ACGT 5 cut(s) 185, 471, 511, 691, 921
HpyF10VI GCNNNNNNNGC 1 cut(s) 113
HpyF3I CTNAG 4 cut(s) 312, 937, 979, 1017
HpySE526I ACGT 5 cut(s) 185, 471, 511, 691, 921
Hsp92II CATG 5 cut(s) 825, 844, 866, 870, 1108
HspAI GCGC 1 cut(s) 20
LmnI GCTCC 1 cut(s) 772
Lsp1109I GCAGC 4 cut(s) 4, 252, 895, 1120
LweI GCATC 2 cut(s) 529, 1077
MaeI CTAG 1 cut(s) 68
MaeII ACGT 5 cut(s) 185, 471, 511, 691, 921
MaeIII GTNAC 2 cut(s) 749, 994
MboII GAAGA 6 cut(s) 46, 52, 632, 709, 953, 1041
MfeI CAATTG 1 cut(s) 327
MflI RGATCY 3 cut(s) 815, 1009, 1110
MhlI GDGCHC 2 cut(s) 714, 901
MluCI AATT 6 cut(s) 134, 175, 234, 327, 366, 583
MlyI GAGTC 2 cut(s) 929, 1022
MmeI TCCRAC 2 cut(s) 316, 408
MnlI CCTC 9 cut(s) 211, 279, 432, 559, 616, 661, 780, 1063, 1138
Mph1103I ATGCAT 1 cut(s) 868
MseI TTAA 3 cut(s) 137, 588, 1116
MslI CAYNNNNRTG 3 cut(s) 417, 470, 826
MspA1I CMGCKG 2 cut(s) 17, 265
MspI CCGG 2 cut(s) 227, 1007
MspR9I CCNGG 2 cut(s) 875, 1007
MunI CAATTG 1 cut(s) 327
MvaI CCWGG 1 cut(s) 875
MwoI GCNNNNNNNGC 1 cut(s) 113
NciI CCSGG 1 cut(s) 1007
NlaIII CATG 5 cut(s) 825, 844, 866, 870, 1108
NlaIV GGNNCC 5 cut(s) 261, 801, 872, 1011, 1044
NmuCI GTSAC 2 cut(s) 749, 994
NsiI ATGCAT 1 cut(s) 868
NspI RCATGY 1 cut(s) 1108
PfeI GAWTC 2 cut(s) 193, 484
PkrI GCNGC 5 cut(s) 16, 19, 267, 885, 1135
PleI GAGTC 2 cut(s) 929, 1022
PmaCI CACGTG 2 cut(s) 472, 692
PmlI CACGTG 2 cut(s) 472, 692
PpsI GAGTC 2 cut(s) 929, 1022
Ppu21I YACGTR 3 cut(s) 186, 472, 692
PpuMI RGGWCCY 1 cut(s) 568
Psp1406I AACGTT 2 cut(s) 511, 921
Psp5II RGGWCCY 1 cut(s) 568
Psp6I CCWGG 1 cut(s) 873
PspCI CACGTG 2 cut(s) 472, 692
PspEI GGTNACC 1 cut(s) 994
PspGI CCWGG 1 cut(s) 873
PspN4I GGNNCC 5 cut(s) 261, 801, 872, 1011, 1044
PspPI GGNCC 4 cut(s) 568, 859, 871, 1004
PspPPI RGGWCCY 1 cut(s) 568
PstI CTGCAG 1 cut(s) 56
PsuI RGATCY 3 cut(s) 815, 1009, 1110
RsaI GTAC 3 cut(s) 125, 542, 839
RsaNI GTAC 3 cut(s) 124, 541, 838
RseI CAYNNNNRTG 3 cut(s) 417, 470, 826
SaqAI TTAA 3 cut(s) 137, 588, 1116
SatI GCNGC 5 cut(s) 15, 18, 266, 884, 1134
Sau96I GGNCC 4 cut(s) 568, 859, 871, 1004
SchI GAGTC 2 cut(s) 929, 1022
ScrFI CCNGG 2 cut(s) 875, 1007
SduI GDGCHC 2 cut(s) 714, 901
SfaNI GCATC 2 cut(s) 529, 1077
SfcI CTRYAG 1 cut(s) 52
SinI GGWCC 2 cut(s) 568, 859
SmiMI CAYNNNNRTG 3 cut(s) 417, 470, 826
SmlI CTYRAG 1 cut(s) 811
SmoI CTYRAG 1 cut(s) 811
Sse9I AATT 6 cut(s) 134, 175, 234, 327, 366, 583
SsiI CCGC 3 cut(s) 15, 84, 263
SspMI CTAG 1 cut(s) 68
StyD4I CCNGG 2 cut(s) 873, 1005
StyI CCWWGG 2 cut(s) 449, 571
TaiI ACGT 5 cut(s) 188, 474, 514, 694, 924
TaqI TCGA 3 cut(s) 910, 1031, 1072
TasI AATT 6 cut(s) 134, 175, 234, 327, 366, 583
TatI WGTACW 2 cut(s) 123, 540
TauI GCSGC 1 cut(s) 17
TfiI GAWTC 2 cut(s) 193, 484
Tru1I TTAA 3 cut(s) 137, 588, 1116
Tru9I TTAA 3 cut(s) 137, 588, 1116
TscAI CASTG 2 cut(s) 691, 745
TseFI GTSAC 2 cut(s) 749, 994
TseI GCWGC 4 cut(s) 17, 265, 883, 1133
Tsp45I GTSAC 2 cut(s) 749, 994
TspDTI ATGAA 9 cut(s) 52, 85, 170, 270, 309, 401, 497, 713, 941
TspRI CASTG 2 cut(s) 691, 745
VneI GTGCAC 2 cut(s) 710, 897
VpaK11BI GGWCC 2 cut(s) 568, 859
XapI RAATTY 2 cut(s) 175, 583
XceI RCATGY 1 cut(s) 1108
XmiI GTMKAC 1 cut(s) 156
XspI CTAG 1 cut(s) 68
Zsp2I ATGCAT 1 cut(s) 868
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.