pycom09g03450

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0006274
Physical Location & Seq
Reverse (-)
2558681 .. 2560325
1645 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 759 bp
ATGGAAATCCCGATGGATGTGATAAAGCAGGTGCAGATCTCGCTTCGGAAAGAAGCGAACATGTCGTCCTACGACCCAGACGACACGCCGTTGCCCAATCTGCCTTCGGTCGAAGAAACCCTGGCGGAGTTGGATCCATCACCGCCGTACCTGAGGTGCAAGCATTGCAAAGGGCGGCTGCTGAGGGGCGTTCAGACGCTGACCTGCGTCTTTTGCGGCAGAGAACTCTGCAAGGACCTGCCTCCTGACCCCGTCAATTTCCGGGATACTTTTGGGTACCGGTGGCTGCTCCAGTCCCTGAGCTTAAGTGGATCGGAGATAGTGGATTTACCTATTGGAGCAAATGAATTAAACAGAGGACAGACTGCACGGAAGGATGAGTTAAGTCTGTCTGATCTCTTAAATCTTGAAATAAAATGGACCTCTAGGCCAGAGAAAATTGAGACTGATCTCTCAAATGAGAACCCCAATCTACCCAAATTACCAGATTTGGCTGGAGTCAATCTCGACAACTTTTTCTTTGAAGGAAACAAAGACCGTGCTTTGGGTAGTTCTGAAGGGCTGTTCGGGTCTAACACACAGATTGTCAGTAAAGAGAGTTTCCAAGTTCATGAAACTCTTAGTTTGTTTCAGAATGTAAAGCCTTTTGAGACAGTTGTGCAGACTACTGAAGGAGAGGGTGGTGATTCTGGTTGGGCCGCAAGTTTTCAGTTTGCTCTTCTGAAACCCTTCCTCGAGCTTCTGAAAACCTTCCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

253

Amino Acids

28.18

Weight (kDa)

4.73

Isoelectric Point (pI)

35.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7815 PF25122 49 - 75 3.9e-15 Domain of unknown function (DUF7815)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 19
Acc36I ACCTGC 3 cut(s) 19, 212, 246
Acc65I GGTACC 1 cut(s) 276
AccB1I GGYRCC 1 cut(s) 276
AciI CCGC 5 cut(s) 125, 143, 175, 216, 699
AclWI GGATC 3 cut(s) 128, 141, 319
AcuI CTGAAG 2 cut(s) 576, 690
AfaI GTAC 2 cut(s) 149, 278
AfiI CCNNNNNNNGG 1 cut(s) 544
AflII CTTAAG 1 cut(s) 304
AflIII ACRYGT 1 cut(s) 60
AgeI ACCGGT 1 cut(s) 279
AgsI TTSAA 2 cut(s) 410, 524
AjnI CCWGG 1 cut(s) 120
AloI GAACNNNNNNTCC 2 cut(s) 50, 82
AluBI AGCT 2 cut(s) 303, 739
AluI AGCT 2 cut(s) 303, 739
Alw26I GTCTC 2 cut(s) 437, 644
AlwI GGATC 3 cut(s) 128, 141, 319
AlwNI CAGNNNCTG 2 cut(s) 199, 298
Ama87I CYCGRG 1 cut(s) 734
AoxI GGCC 2 cut(s) 428, 696
ApeKI GCWGC 2 cut(s) 178, 286
ArsI GACNNNNNNTTYG 2 cut(s) 50, 82
AsiGI ACCGGT 1 cut(s) 279
Asp700I GAANNNNTTC 2 cut(s) 728, 749
Asp718I GGTACC 1 cut(s) 276
AspS9I GGNCC 3 cut(s) 235, 420, 696
AsuC2I CCSGG 1 cut(s) 263
AsuHPI GGTGA 2 cut(s) 132, 695
AvaI CYCGRG 1 cut(s) 734
AvaII GGWCC 2 cut(s) 235, 420
AxyI CCTNAGG 1 cut(s) 152
BaeI ACNNNNGTAYC 2 cut(s) 131, 164
BamHI GGATCC 1 cut(s) 133
BanI GGYRCC 1 cut(s) 276
BbvCI CCTCAGC 1 cut(s) 182
BbvI GCAGC 2 cut(s) 165, 273
BccI CCATC 2 cut(s) 7, 145
BceAI ACGGC 2 cut(s) 73, 130
BciT130I CCWGG 1 cut(s) 122
BciVI GTATCC 1 cut(s) 259
BcnI CCSGG 1 cut(s) 263
BcoDI GTCTC 2 cut(s) 437, 644
BfaI CTAG 1 cut(s) 426
BfrI CTTAAG 1 cut(s) 304
BfuAI ACCTGC 3 cut(s) 19, 212, 246
BfuI GTATCC 1 cut(s) 259
BglII AGATCT 1 cut(s) 36
BisI GCNGC 5 cut(s) 176, 179, 217, 287, 699
BlsI GCNGC 5 cut(s) 177, 180, 218, 288, 700
Bme1390I CCNGG 2 cut(s) 122, 263
Bme18I GGWCC 2 cut(s) 235, 420
BmeT110I CYCGRG 1 cut(s) 734
BmgT120I GGNCC 3 cut(s) 235, 420, 696
BmiI GGNNCC 2 cut(s) 135, 278
BmrFI CCNGG 2 cut(s) 122, 263
BoxI GACNNNNGTC 1 cut(s) 206
BplI GAGNNNNNCTC 2 cut(s) 489, 521
BpmI CTGGAG 2 cut(s) 275, 516
Bpu10I CCTNAGC 2 cut(s) 182, 299
BpuMI CCSGG 1 cut(s) 263
BsaJI CCNNGG 1 cut(s) 120
BsaWI WCCGGW 1 cut(s) 279
Bsc4I CCNNNNNNNGG 1 cut(s) 544
Bse118I RCCGGY 1 cut(s) 279
Bse1I ACTGG 1 cut(s) 292
Bse21I CCTNAGG 1 cut(s) 152
Bse3DI GCAATG 1 cut(s) 163
BseBI CCWGG 1 cut(s) 122
BseDI CCNNGG 1 cut(s) 120
BseGI GGATG 2 cut(s) 22, 382
BseLI CCNNNNNNNGG 1 cut(s) 544
BseMI GCAATG 1 cut(s) 163
BseMII CTCAG 3 cut(s) 143, 173, 290
BseNI ACTGG 1 cut(s) 292
BseXI GCAGC 2 cut(s) 165, 273
BsgI GTGCAG 3 cut(s) 53, 351, 680
Bsh1285I CGRYCG 1 cut(s) 111
BshFI GGCC 2 cut(s) 430, 698
BshNI GGYRCC 1 cut(s) 276
BshTI ACCGGT 1 cut(s) 279
BsiEI CGRYCG 1 cut(s) 111
BsiHKCI CYCGRG 1 cut(s) 734
BsiSI CCGG 2 cut(s) 262, 280
BslFI GGGAC 1 cut(s) 280
BslI CCNNNNNNNGG 1 cut(s) 544
BsmAI GTCTC 2 cut(s) 437, 644
BsmFI GGGAC 1 cut(s) 280
BsnI GGCC 2 cut(s) 430, 698
BsoBI CYCGRG 1 cut(s) 734
Bsp143I GATC 5 cut(s) 36, 133, 311, 394, 448
BspACI CCGC 5 cut(s) 125, 143, 175, 216, 699
BspANI GGCC 2 cut(s) 430, 698
BspCNI CTCAG 3 cut(s) 144, 174, 291
BspHI TCATGA 1 cut(s) 610
BspLI GGNNCC 2 cut(s) 135, 278
BspMI ACCTGC 3 cut(s) 19, 212, 246
BspPI GGATC 3 cut(s) 128, 141, 319
BspQI GCTCTTC 1 cut(s) 723
BspT107I GGYRCC 1 cut(s) 276
BspTI CTTAAG 1 cut(s) 304
BsrDI GCAATG 1 cut(s) 163
BsrFI RCCGGY 1 cut(s) 279
BsrI ACTGG 1 cut(s) 292
BssAI RCCGGY 1 cut(s) 279
BssECI CCNNGG 1 cut(s) 120
BssMI GATC 5 cut(s) 36, 133, 311, 394, 448
Bst2UI CCWGG 1 cut(s) 122
Bst4CI ACNGT 2 cut(s) 539, 655
Bst6I CTCTTC 1 cut(s) 723
BstAFI CTTAAG 1 cut(s) 304
BstAPI GCANNNNNTGC 1 cut(s) 165
BstC8I GCNNGC 1 cut(s) 161
BstDEI CTNAG 4 cut(s) 152, 182, 299, 620
BstF5I GGATG 2 cut(s) 22, 382
BstKTI GATC 5 cut(s) 39, 136, 314, 397, 451
BstMAI GTCTC 2 cut(s) 437, 644
BstMBI GATC 5 cut(s) 36, 133, 311, 394, 448
BstMCI CGRYCG 1 cut(s) 111
BstMWI GCNNNNNNNGC 4 cut(s) 40, 100, 165, 213
BstNI CCWGG 1 cut(s) 122
BstNSI RCATGY 1 cut(s) 64
BstPAI GACNNNNGTC 1 cut(s) 206
BstSCI CCNGG 2 cut(s) 120, 261
BstV1I GCAGC 2 cut(s) 165, 273
BstX2I RGATCY 2 cut(s) 36, 133
BstYI RGATCY 2 cut(s) 36, 133
Bsu36I CCTNAGG 1 cut(s) 152
BsuI GTATCC 1 cut(s) 259
BsuRI GGCC 2 cut(s) 430, 698
BtsCI GGATG 2 cut(s) 22, 382
BveI ACCTGC 3 cut(s) 19, 212, 246
Cac8I GCNNGC 1 cut(s) 161
CaiI CAGNNNCTG 2 cut(s) 199, 298
CciI TCATGA 1 cut(s) 610
Cfr10I RCCGGY 1 cut(s) 279
Cfr13I GGNCC 3 cut(s) 235, 420, 696
CseI GACGC 2 cut(s) 196, 205
Csp6I GTAC 2 cut(s) 148, 277
CspAI ACCGGT 1 cut(s) 279
CviAII CATG 2 cut(s) 61, 611
CviJI RGCY 9 cut(s) 178, 286, 303, 430, 494, 562, 643, 698, 739
CviKI_1 RGCY 9 cut(s) 178, 286, 303, 430, 494, 562, 643, 698, 739
CviQI GTAC 2 cut(s) 148, 277
DdeI CTNAG 4 cut(s) 152, 182, 299, 620
DpnI GATC 5 cut(s) 38, 135, 313, 396, 450
DpnII GATC 5 cut(s) 36, 133, 311, 394, 448
Eam1104I CTCTTC 1 cut(s) 723
EarI CTCTTC 1 cut(s) 723
EciI GGCGGA 1 cut(s) 140
Eco47I GGWCC 2 cut(s) 235, 420
Eco57I CTGAAG 2 cut(s) 576, 690
Eco81I CCTNAGG 1 cut(s) 152
Eco88I CYCGRG 1 cut(s) 734
EcoO109I RGGNCCY 1 cut(s) 235
EcoRII CCWGG 1 cut(s) 120
FaeI CATG 2 cut(s) 64, 614
FaiI YATR 2 cut(s) 62, 612
FaqI GGGAC 1 cut(s) 280
FatI CATG 2 cut(s) 60, 610
Fnu4HI GCNGC 5 cut(s) 176, 179, 217, 287, 699
FokI GGATG 2 cut(s) 29, 389
Fsp4HI GCNGC 5 cut(s) 176, 179, 217, 287, 699
FspBI CTAG 1 cut(s) 426
GluI GCNGC 5 cut(s) 176, 179, 217, 287, 699
GsuI CTGGAG 2 cut(s) 275, 516
HaeIII GGCC 2 cut(s) 430, 698
HapII CCGG 2 cut(s) 262, 280
HgaI GACGC 2 cut(s) 196, 205
Hin1II CATG 2 cut(s) 64, 614
HinfI GANTC 2 cut(s) 498, 686
HpaII CCGG 2 cut(s) 262, 280
HphI GGTGA 2 cut(s) 132, 695
Hpy188I TCNGA 8 cut(s) 48, 195, 316, 394, 556, 633, 723, 744
Hpy188III TCNNGA 5 cut(s) 10, 245, 407, 506, 611
HpyAV CCTTC 6 cut(s) 114, 367, 518, 551, 665, 739
HpyCH4III ACNGT 2 cut(s) 539, 655
HpyCH4V TGCA 6 cut(s) 34, 159, 168, 231, 368, 661
HpyF10VI GCNNNNNNNGC 4 cut(s) 40, 100, 165, 213
HpyF3I CTNAG 4 cut(s) 152, 182, 299, 620
Hsp92II CATG 2 cut(s) 64, 614
KpnI GGTACC 1 cut(s) 280
Kzo9I GATC 5 cut(s) 36, 133, 311, 394, 448
LguI GCTCTTC 1 cut(s) 723
LmnI GCTCC 2 cut(s) 294, 338
Lsp1109I GCAGC 2 cut(s) 165, 273
MaeI CTAG 1 cut(s) 426
MalI GATC 5 cut(s) 38, 135, 313, 396, 450
MboI GATC 5 cut(s) 36, 133, 311, 394, 448
MboII GAAGA 2 cut(s) 125, 710
MflI RGATCY 2 cut(s) 36, 133
MluCI AATT 4 cut(s) 256, 347, 438, 479
MlyI GAGTC 1 cut(s) 507
MmeI TCCRAC 1 cut(s) 111
MnlI CCTC 7 cut(s) 147, 177, 252, 350, 433, 670, 743
MroXI GAANNNNTTC 2 cut(s) 728, 749
MseI TTAA 4 cut(s) 305, 350, 383, 401
MspCI CTTAAG 1 cut(s) 304
MspI CCGG 2 cut(s) 262, 280
MspR9I CCNGG 2 cut(s) 122, 263
MvaI CCWGG 1 cut(s) 122
MwoI GCNNNNNNNGC 4 cut(s) 40, 100, 165, 213
NciI CCSGG 1 cut(s) 263
NdeII GATC 5 cut(s) 36, 133, 311, 394, 448
NlaIII CATG 2 cut(s) 64, 614
NlaIV GGNNCC 2 cut(s) 135, 278
NspI RCATGY 1 cut(s) 64
PaeR7I CTCGAG 1 cut(s) 734
PagI TCATGA 1 cut(s) 610
PaqCI CACCTGC 1 cut(s) 19
PciI ACATGT 1 cut(s) 60
PciSI GCTCTTC 1 cut(s) 723
PcsI WCGNNNNNNNCGW 1 cut(s) 78
PdmI GAANNNNTTC 2 cut(s) 728, 749
PfeI GAWTC 1 cut(s) 686
PflFI GACNNNGTC 1 cut(s) 251
PfoI TCCNGGA 1 cut(s) 261
PinAI ACCGGT 1 cut(s) 279
PkrI GCNGC 5 cut(s) 177, 180, 218, 288, 700
PleI GAGTC 1 cut(s) 506
PpsI GAGTC 1 cut(s) 506
PpuMI RGGWCCY 1 cut(s) 235
PscI ACATGT 1 cut(s) 60
PshAI GACNNNNGTC 1 cut(s) 206
Psp5II RGGWCCY 1 cut(s) 235
Psp6I CCWGG 1 cut(s) 120
PspGI CCWGG 1 cut(s) 120
PspN4I GGNNCC 2 cut(s) 135, 278
PspPI GGNCC 3 cut(s) 235, 420, 696
PspPPI RGGWCCY 1 cut(s) 235
PspXI VCTCGAGB 1 cut(s) 734
PstNI CAGNNNCTG 2 cut(s) 199, 298
PsuI RGATCY 2 cut(s) 36, 133
PsyI GACNNNGTC 1 cut(s) 251
RsaI GTAC 2 cut(s) 149, 278
RsaNI GTAC 2 cut(s) 148, 277
SapI GCTCTTC 1 cut(s) 723
SaqAI TTAA 4 cut(s) 305, 350, 383, 401
SatI GCNGC 5 cut(s) 176, 179, 217, 287, 699
Sau3AI GATC 5 cut(s) 36, 133, 311, 394, 448
Sau96I GGNCC 3 cut(s) 235, 420, 696
SchI GAGTC 1 cut(s) 507
ScrFI CCNGG 2 cut(s) 122, 263
Sfr274I CTCGAG 1 cut(s) 734
SinI GGWCC 2 cut(s) 235, 420
SlaI CTCGAG 1 cut(s) 734
SmlI CTYRAG 2 cut(s) 304, 734
SmoI CTYRAG 2 cut(s) 304, 734
Sse9I AATT 4 cut(s) 256, 347, 438, 479
SsiI CCGC 5 cut(s) 125, 143, 175, 216, 699
SspMI CTAG 1 cut(s) 426
StyD4I CCNGG 2 cut(s) 120, 261
TaaI ACNGT 2 cut(s) 539, 655
TaqI TCGA 3 cut(s) 111, 507, 735
TaqII GACCGA 1 cut(s) 97
TasI AATT 4 cut(s) 256, 347, 438, 479
TauI GCSGC 3 cut(s) 178, 219, 701
TfiI GAWTC 1 cut(s) 686
Tru1I TTAA 4 cut(s) 305, 350, 383, 401
Tru9I TTAA 4 cut(s) 305, 350, 383, 401
TseI GCWGC 2 cut(s) 178, 286
TspDTI ATGAA 3 cut(s) 360, 599, 627
TspGWI ACGGA 1 cut(s) 385
Tth111I GACNNNGTC 1 cut(s) 251
Vha464I CTTAAG 1 cut(s) 304
VpaK11BI GGWCC 2 cut(s) 235, 420
XceI RCATGY 1 cut(s) 64
XhoI CTCGAG 1 cut(s) 734
XmnI GAANNNNTTC 2 cut(s) 728, 749
XspI CTAG 1 cut(s) 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.