pycom09g05160

Nitrile-specifier protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0005031
Physical Location & Seq
Reverse (-)
3830470 .. 3832475
2006 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 987 bp
ATGTCTCTGGTTCAAGGCAAATGGATCAAGCTTGATCAAAAGGGGACTGGTCCTGGAGCTAGAAGCTCACATGCCATTACCCTAGTAGGACAGAAGGCCTATGTTTTCGGAGGTGAATTCACGCCACGTGTCCCGGTAGACAACAAGATCCATGTGTTCGATGTCAAGGAGCAGACATGGTATGTGACTGAAGGAACCGGGGATGTCCCACCGCCACGTGTTGGTGTGACATTGGTAACTGTTGGAGAAATTATTTATGTATTTGGAGGTAGGGATTATGAACATAATGAGCTCAATGAGCTCTATTCCTTTGACACATCCACACACAAGTGGACTCTAATTTCAAGTGGGGACACCGGGCCCCCTCACCGGAGTTACCACTCAGTCACCTCCGATGACCACCACATATACATCTTCGGCGGATGTGGTGTTGTCGGCCGCCTCAATGATCTATGGGCCTACAATGTCGTTGATCAAAAGTGGATCCAATACCCAAATCCGGGTGACAACTTGAAGGGGAGGGGTGGGGCGGGCCTGCTAGAGGTCCAAGGAAAAATATGGGTTGTGTATGGCTTCGCTGGAGAGGAAGTGGATGATGTACACATCTTTGATCCAGCCCATGGTGAGTGGGCCCAAGTTGAGACAAATGGCGAGAAACCAACGGCTCGGAGTGTATTTTCTACGGTAGCAATCGGTAAATACATAATCATATACGGTGGTGAAGTGGACCCTAGTGACTTGGGCCACTTGGGTGCTGGGAAATTTGTTGCGGAGGTTTATGCATTGGACACAGAAACATTGTTGTGGAAGAAGTGGGACGATGACCTAGGCTCAGGCCATCATCCCGGCCCTCGTGGGTGGTGTGCATTTGCCCGCGGGCAGTGGGAGGGGAAAGAGGGCCTTTTGGTGTATGGTGGGAATTCACCAACTAATGATCGACTTGGTGATATTCATTTCTTCACTCCTTACTTGGAAAATGGTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kelch_1 Kelch_3 Kelch_4

Protein Analysis

329

Amino Acids

36.13

Weight (kDa)

5.4

Isoelectric Point (pI)

15.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_ATRN-LZTR1 PF24981 4 - 82 4.1e-09 Attractin/LZTR1 beta-propeller
Beta-prop_FBX42 PF13415 5 - 144 5.6e-28 FBX42, beta-propeller domain
Beta-prop_Calicin PF13964 7 - 146 2.5e-08 Calicin, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 23 - 240 1.3e-68 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_HCF PF13854 48 - 141 1e-07 Host cell factor, Kelch-repeats domain
Kelch_FKB95 PF25210 70 - 235 1.8e-11 FKB95, Kelch-repeats domain
Kelch_1 PF01344 72 - 116 2e-11 Kelch motif
Beta-prop_ATRN-LZTR1 PF24981 85 - 165 8.5e-16 Attractin/LZTR1 beta-propeller
Kelch_1 PF01344 123 - 167 3.8e-10 Kelch motif
Beta-prop_FBX42 PF13415 148 - 321 5.2e-17 FBX42, beta-propeller domain
Kelch_1 PF01344 174 - 214 3.2e-06 Kelch motif
Beta-prop_ATRN-LZTR1 PF24981 186 - 313 3.8e-11 Attractin/LZTR1 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 221, 620
AccI GTMKAC 1 cut(s) 138
AccII CGCG 1 cut(s) 876
AciI CCGC 7 cut(s) 212, 420, 439, 530, 770, 874, 876
AclWI GGATC 5 cut(s) 32, 142, 478, 491, 605
AcoI YGGCCR 1 cut(s) 436
AcsI RAATTY 3 cut(s) 116, 761, 919
AcuI CTGAAG 1 cut(s) 210
AcvI CACGTG 2 cut(s) 128, 218
AfaI GTAC 1 cut(s) 600
AfiI CCNNNNNNNGG 6 cut(s) 221, 369, 499, 500, 541, 620
AflIII ACRYGT 2 cut(s) 127, 217
AgsI TTSAA 3 cut(s) 14, 345, 514
AjnI CCWGG 1 cut(s) 52
AleI CACNNNNGTG 2 cut(s) 328, 750
AluBI AGCT 5 cut(s) 31, 59, 66, 292, 301
AluI AGCT 5 cut(s) 31, 59, 66, 292, 301
Alw21I GWGCWC 2 cut(s) 294, 303
Alw26I GTCTC 2 cut(s) 9, 635
AlwI GGATC 5 cut(s) 32, 142, 478, 491, 605
ApaI GGGCCC 2 cut(s) 363, 634
ApoI RAATTY 3 cut(s) 116, 761, 919
AspA2I CCTAGG 1 cut(s) 826
AsuC2I CCSGG 5 cut(s) 134, 199, 358, 501, 846
AsuHPI GGTGA 8 cut(s) 125, 359, 379, 515, 635, 731, 915, 956
AvaII GGWCC 3 cut(s) 50, 544, 727
AvrII CCTAGG 1 cut(s) 826
BaeGI GKGCMC 2 cut(s) 363, 634
BamHI GGATCC 1 cut(s) 483
BanII GRGCYC 4 cut(s) 294, 303, 363, 634
BauI CACGAG 1 cut(s) 852
BbrPI CACGTG 2 cut(s) 128, 218
Bbv12I GWGCWC 2 cut(s) 294, 303
BccI CCATC 1 cut(s) 846
BceAI ACGGC 1 cut(s) 678
BciT130I CCWGG 1 cut(s) 54
BclI TGATCA 2 cut(s) 34, 472
BcnI CCSGG 5 cut(s) 134, 199, 358, 501, 846
BcoDI GTCTC 2 cut(s) 9, 635
BfaI CTAG 5 cut(s) 60, 83, 539, 732, 827
BisI GCNGC 1 cut(s) 439
BlnI CCTAGG 1 cut(s) 826
BlsI GCNGC 1 cut(s) 440
Bme1390I CCNGG 6 cut(s) 54, 134, 199, 358, 501, 846
Bme18I GGWCC 3 cut(s) 50, 544, 727
BmiI GGNNCC 6 cut(s) 196, 361, 362, 485, 632, 729
BmrFI CCNGG 6 cut(s) 54, 134, 199, 358, 501, 846
BpmI CTGGAG 2 cut(s) 75, 600
Bpu10I CCTNAGC 1 cut(s) 832
BpuMI CCSGG 5 cut(s) 134, 199, 358, 501, 846
BsaAI YACGTR 2 cut(s) 128, 218
BsaJI CCNNGG 5 cut(s) 198, 547, 619, 826, 874
BsaWI WCCGGW 1 cut(s) 369
BsaXI ACNNNNNCTCC 2 cut(s) 573, 603
Bsc4I CCNNNNNNNGG 6 cut(s) 221, 369, 499, 500, 541, 620
Bse1I ACTGG 1 cut(s) 52
BseBI CCWGG 1 cut(s) 54
BseDI CCNNGG 5 cut(s) 198, 547, 619, 826, 874
BseGI GGATG 5 cut(s) 208, 317, 428, 598, 841
BseLI CCNNNNNNNGG 6 cut(s) 221, 369, 499, 500, 541, 620
BseMII CTCAG 2 cut(s) 396, 846
BseNI ACTGG 1 cut(s) 52
BseSI GKGCMC 2 cut(s) 363, 634
BseX3I CGGCCG 1 cut(s) 436
BseYI CCCAGC 1 cut(s) 755
Bsh1236I CGCG 1 cut(s) 876
Bsh1285I CGRYCG 1 cut(s) 439
BsiEI CGRYCG 1 cut(s) 439
BsiHKAI GWGCWC 2 cut(s) 294, 303
BsiSI CCGG 6 cut(s) 134, 198, 357, 370, 500, 846
BslFI GGGAC 5 cut(s) 58, 116, 191, 365, 830
BslI CCNNNNNNNGG 6 cut(s) 221, 369, 499, 500, 541, 620
BsmAI GTCTC 2 cut(s) 9, 635
BsmFI GGGAC 5 cut(s) 58, 116, 191, 365, 830
Bsp120I GGGCCC 2 cut(s) 359, 630
Bsp1286I GDGCHC 4 cut(s) 294, 303, 363, 634
Bsp1407I TGTACA 1 cut(s) 598
Bsp143I GATC 8 cut(s) 24, 34, 147, 448, 472, 483, 610, 934
Bsp19I CCATGG 1 cut(s) 619
BspACI CCGC 7 cut(s) 212, 420, 439, 530, 770, 874, 876
BspCNI CTCAG 2 cut(s) 395, 845
BspFNI CGCG 1 cut(s) 876
BspLI GGNNCC 6 cut(s) 196, 361, 362, 485, 632, 729
BspPI GGATC 5 cut(s) 32, 142, 478, 491, 605
BsrGI TGTACA 1 cut(s) 598
BsrI ACTGG 1 cut(s) 52
BssECI CCNNGG 5 cut(s) 198, 547, 619, 826, 874
BssMI GATC 8 cut(s) 24, 34, 147, 448, 472, 483, 610, 934
BssSI CACGAG 1 cut(s) 852
BssT1I CCWWGG 3 cut(s) 547, 619, 826
Bst2BI CACGAG 1 cut(s) 852
Bst2UI CCWGG 1 cut(s) 54
Bst4CI ACNGT 3 cut(s) 241, 685, 716
BstAUI TGTACA 1 cut(s) 598
BstBAI YACGTR 2 cut(s) 128, 218
BstC8I GCNNGC 4 cut(s) 532, 536, 874, 878
BstDEI CTNAG 2 cut(s) 382, 832
BstDSI CCRYGG 2 cut(s) 619, 874
BstENI CCTNNNNNAGG 1 cut(s) 539
BstF5I GGATG 5 cut(s) 208, 317, 428, 598, 841
BstFNI CGCG 1 cut(s) 876
BstKTI GATC 8 cut(s) 27, 37, 150, 451, 475, 486, 613, 937
BstMAI GTCTC 2 cut(s) 9, 635
BstMBI GATC 8 cut(s) 24, 34, 147, 448, 472, 483, 610, 934
BstMCI CGRYCG 1 cut(s) 439
BstMWI GCNNNNNNNGC 1 cut(s) 298
BstNI CCWGG 1 cut(s) 54
BstNSI RCATGY 1 cut(s) 74
BstSCI CCNGG 6 cut(s) 52, 132, 197, 356, 499, 844
BstSLI GKGCMC 2 cut(s) 363, 634
BstUI CGCG 1 cut(s) 876
BstX2I RGATCY 2 cut(s) 147, 483
BstYI RGATCY 2 cut(s) 147, 483
BstZI CGGCCG 1 cut(s) 436
BtgI CCRYGG 2 cut(s) 619, 874
BtsCI GGATG 5 cut(s) 208, 317, 428, 598, 841
BtsI GCAGTG 1 cut(s) 887
BtsIMutI CAGTG 1 cut(s) 887
Cac8I GCNNGC 4 cut(s) 532, 536, 874, 878
Cfr42I CCGCGG 1 cut(s) 877
Csp6I GTAC 1 cut(s) 599
CviAII CATG 4 cut(s) 71, 152, 177, 620
CviQI GTAC 1 cut(s) 599
DdeI CTNAG 2 cut(s) 382, 832
DpnI GATC 8 cut(s) 26, 36, 149, 450, 474, 485, 612, 936
DpnII GATC 8 cut(s) 24, 34, 147, 448, 472, 483, 610, 934
EaeI YGGCCR 1 cut(s) 436
EagI CGGCCG 1 cut(s) 436
EciI GGCGGA 1 cut(s) 435
Ecl136II GAGCTC 2 cut(s) 292, 301
EclXI CGGCCG 1 cut(s) 436
Eco130I CCWWGG 3 cut(s) 547, 619, 826
Eco147I AGGCCT 1 cut(s) 98
Eco24I GRGCYC 4 cut(s) 294, 303, 363, 634
Eco47I GGWCC 3 cut(s) 50, 544, 727
Eco52I CGGCCG 1 cut(s) 436
Eco53kI GAGCTC 2 cut(s) 292, 301
Eco57I CTGAAG 1 cut(s) 210
Eco72I CACGTG 2 cut(s) 128, 218
EcoICRI GAGCTC 2 cut(s) 292, 301
EcoNI CCTNNNNNAGG 1 cut(s) 539
EcoO109I RGGNCCY 2 cut(s) 360, 898
EcoRI GAATTC 2 cut(s) 116, 919
EcoRII CCWGG 1 cut(s) 52
EcoT14I CCWWGG 3 cut(s) 547, 619, 826
EcoT22I ATGCAT 1 cut(s) 784
EcoT38I GRGCYC 4 cut(s) 294, 303, 363, 634
ErhI CCWWGG 3 cut(s) 547, 619, 826
FaeI CATG 4 cut(s) 74, 155, 180, 623
FalI AAGNNNNNCTT 2 cut(s) 885, 917
FaqI GGGAC 5 cut(s) 58, 116, 191, 365, 830
FatI CATG 4 cut(s) 70, 151, 176, 619
FauI CCCGC 3 cut(s) 523, 869, 881
FbaI TGATCA 2 cut(s) 34, 472
FblI GTMKAC 1 cut(s) 138
Fnu4HI GCNGC 1 cut(s) 439
FokI GGATG 5 cut(s) 215, 304, 435, 605, 828
FriOI GRGCYC 4 cut(s) 294, 303, 363, 634
Fsp4HI GCNGC 1 cut(s) 439
FspBI CTAG 5 cut(s) 60, 83, 539, 732, 827
GluI GCNGC 1 cut(s) 439
GsaI CCCAGC 1 cut(s) 759
GsuI CTGGAG 2 cut(s) 75, 600
HapII CCGG 6 cut(s) 134, 198, 357, 370, 500, 846
Hin1II CATG 4 cut(s) 74, 155, 180, 623
HindIII AAGCTT 1 cut(s) 29
HinfI GANTC 1 cut(s) 334
HpaII CCGG 6 cut(s) 134, 198, 357, 370, 500, 846
HphI GGTGA 8 cut(s) 125, 359, 379, 515, 635, 731, 915, 956
Hpy166II GTNNAC 4 cut(s) 139, 333, 601, 727
Hpy188I TCNGA 3 cut(s) 110, 394, 669
Hpy8I GTNNAC 4 cut(s) 139, 333, 601, 727
HpyAV CCTTC 3 cut(s) 88, 185, 508
HpyCH4III ACNGT 3 cut(s) 241, 685, 716
HpyCH4IV ACGT 2 cut(s) 127, 217
HpyCH4V TGCA 2 cut(s) 782, 866
HpyF10VI GCNNNNNNNGC 1 cut(s) 298
HpyF3I CTNAG 2 cut(s) 382, 832
HpySE526I ACGT 2 cut(s) 127, 217
Hsp92II CATG 4 cut(s) 74, 155, 180, 623
Ksp22I TGATCA 2 cut(s) 34, 472
KspI CCGCGG 1 cut(s) 877
Kzo9I GATC 8 cut(s) 24, 34, 147, 448, 472, 483, 610, 934
LmnI GCTCC 2 cut(s) 56, 169
MaeI CTAG 5 cut(s) 60, 83, 539, 732, 827
MaeII ACGT 2 cut(s) 127, 217
MaeIII GTNAC 7 cut(s) 184, 226, 235, 374, 385, 503, 734
MalI GATC 8 cut(s) 26, 36, 149, 450, 474, 485, 612, 936
MboI GATC 8 cut(s) 24, 34, 147, 448, 472, 483, 610, 934
MboII GAAGA 3 cut(s) 406, 820, 949
MflI RGATCY 2 cut(s) 147, 483
MhlI GDGCHC 4 cut(s) 294, 303, 363, 634
MluCI AATT 5 cut(s) 116, 249, 339, 761, 919
MlyI GAGTC 1 cut(s) 328
MmeI TCCRAC 1 cut(s) 223
Mph1103I ATGCAT 1 cut(s) 784
MslI CAYNNNNRTG 3 cut(s) 328, 750, 802
MspA1I CMGCKG 1 cut(s) 876
MspI CCGG 6 cut(s) 134, 198, 357, 370, 500, 846
MspR9I CCNGG 6 cut(s) 54, 134, 199, 358, 501, 846
MvaI CCWGG 1 cut(s) 54
MvnI CGCG 1 cut(s) 876
MwoI GCNNNNNNNGC 1 cut(s) 298
NciI CCSGG 5 cut(s) 134, 199, 358, 501, 846
NcoI CCATGG 1 cut(s) 619
NdeII GATC 8 cut(s) 24, 34, 147, 448, 472, 483, 610, 934
NlaIII CATG 4 cut(s) 74, 155, 180, 623
NlaIV GGNNCC 6 cut(s) 196, 361, 362, 485, 632, 729
NmuCI GTSAC 5 cut(s) 184, 226, 385, 503, 734
NsiI ATGCAT 1 cut(s) 784
NspI RCATGY 1 cut(s) 74
OliI CACNNNNGTG 2 cut(s) 328, 750
PceI AGGCCT 1 cut(s) 98
PflMI CCANNNNNTGG 2 cut(s) 221, 620
PfoI TCCNGGA 1 cut(s) 52
PkrI GCNGC 1 cut(s) 440
PleI GAGTC 1 cut(s) 328
PmaCI CACGTG 2 cut(s) 128, 218
PmlI CACGTG 2 cut(s) 128, 218
PpsI GAGTC 1 cut(s) 328
Ppu21I YACGTR 2 cut(s) 128, 218
Psp124BI GAGCTC 2 cut(s) 294, 303
Psp6I CCWGG 1 cut(s) 52
PspCI CACGTG 2 cut(s) 128, 218
PspFI CCCAGC 1 cut(s) 755
PspGI CCWGG 1 cut(s) 52
PspN4I GGNNCC 6 cut(s) 196, 361, 362, 485, 632, 729
PspOMI GGGCCC 2 cut(s) 359, 630
PsuI RGATCY 2 cut(s) 147, 483
RsaI GTAC 1 cut(s) 600
RsaNI GTAC 1 cut(s) 599
RseI CAYNNNNRTG 3 cut(s) 328, 750, 802
SacI GAGCTC 2 cut(s) 294, 303
SacII CCGCGG 1 cut(s) 877
SatI GCNGC 1 cut(s) 439
Sau3AI GATC 8 cut(s) 24, 34, 147, 448, 472, 483, 610, 934
SchI GAGTC 1 cut(s) 328
ScrFI CCNGG 6 cut(s) 54, 134, 199, 358, 501, 846
SduI GDGCHC 4 cut(s) 294, 303, 363, 634
Sfr303I CCGCGG 1 cut(s) 877
SgrBI CCGCGG 1 cut(s) 877
SinI GGWCC 3 cut(s) 50, 544, 727
SmiMI CAYNNNNRTG 3 cut(s) 328, 750, 802
Sse9I AATT 5 cut(s) 116, 249, 339, 761, 919
SseBI AGGCCT 1 cut(s) 98
SsiI CCGC 7 cut(s) 212, 420, 439, 530, 770, 874, 876
SspMI CTAG 5 cut(s) 60, 83, 539, 732, 827
SstI GAGCTC 2 cut(s) 294, 303
StuI AGGCCT 1 cut(s) 98
StyD4I CCNGG 6 cut(s) 52, 132, 197, 356, 499, 844
StyI CCWWGG 3 cut(s) 547, 619, 826
TaaI ACNGT 3 cut(s) 241, 685, 716
TaiI ACGT 2 cut(s) 130, 220
TaqI TCGA 2 cut(s) 159, 937
TasI AATT 5 cut(s) 116, 249, 339, 761, 919
TatI WGTACW 1 cut(s) 598
TauI GCSGC 1 cut(s) 441
TscAI CASTG 1 cut(s) 887
TseFI GTSAC 5 cut(s) 184, 226, 385, 503, 734
Tsp45I GTSAC 5 cut(s) 184, 226, 385, 503, 734
TspDTI ATGAA 2 cut(s) 294, 941
TspRI CASTG 1 cut(s) 887
Van91I CCANNNNNTGG 2 cut(s) 221, 620
VpaK11BI GGWCC 3 cut(s) 50, 544, 727
XagI CCTNNNNNAGG 1 cut(s) 539
XapI RAATTY 3 cut(s) 116, 761, 919
XceI RCATGY 1 cut(s) 74
XcmI CCANNNNNNNNNTGG 1 cut(s) 752
XmaJI CCTAGG 1 cut(s) 826
XmiI GTMKAC 1 cut(s) 138
XspI CTAG 5 cut(s) 60, 83, 539, 732, 827
Zsp2I ATGCAT 1 cut(s) 784
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.