pycom09g06840
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB NOP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0013335
Physical Location & Seq
Reverse (-)
5062122 .. 5063984
1863 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 873 bp
ATGAAGAAGAAAGCTAGCAACTTGCTCAGAACCATCACCACCTTCAACCCCAAGCACCGCAAAATCATGGACGATTTACCAGAGCGCTACTCTTACAACCCAACTCTCCGCTGGAACGCCGACCTCGAAAGTTACTTCTCCAACGCCTACGGACCCCACCATTTCTCCCGCATCTCCGATGCACTAACGCGGCCGTCTTGCTACTCCTGCATACGCGTGAACACGCTGAAGTCGACGCCGGATGCGGCCATTGAGAAGCTGAGGACGGTTCTGAAAGAAAAAGGAGCTGACATTTCCAAGTGTGAAGTGGGAGGGATGGACTATGTTCTGTTTGTGAAGGGTTCGGGCCCGCAGGCCATTGATTATAGCTGTGCTGCAGATGGGAAGCCTCCGAAAGAGGTTCTTGTGAGCCGGAAATGTGCCGAGGCAGTTCTTCGAGGTGCCCAGGTGTATGTTCCTGGTGTACTGGGTTGCACTGCTCGTGTCGAGAAAGGGGATATTGTGGCGGTTTCAGTTGCGGTTCAGCAGCCCGGTGCTGATGGTGGATGGGTCGTTGGGATCACCCGAGGGACTGTGCCGCAAGGTTCAGAGACAGATCCTTATCATCTTGAACGAGGTGGGCTGTATATCGGCAAAGGAAGAGCAACGCTGTCAAGGGCCGGGATTTTCTGTGTTATGGAAGGGCTTGCTGTGGATATGAGTGTCAGAGTTTTTAATCTTCCTTCTTTACATGATGTGCTTGAGGAAGAAATATTTCTTCAAAATCTGCCAATCATTATTGTTGCTCATGCACTAGATCCTCAAAAAGGTGAACGCATATTAGACTTGTGTCTGCACCAGGAGGGAAAACAACTGCAATTGCACTTCTTATGA

Protein Analysis

291

Amino Acids

31.84

Weight (kDa)

8.35

Isoelectric Point (pI)

33.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUA PF01472 133 - 170 2.5e-06 PUA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013335)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G06560
fragaria_vesca FvH4_6g38630 FvH4_6g38630
malus_domestica MD09G1148800.v1.1 MD17G1135100.v1.1
prunus_persica Prupe.3G177300_v2.0.a1 Prupe.3G177300_v2.0.a1
pyrus_communis pycom09g06840 pycom17g12920
rosa_chinensis RchiOBHm_Chr2g0152091
rosa_laevigata RLG00000020607
rosa_multiflora Rmu_co8372479.1_g000001 Rmu_sc0000760.1_g000014
rosa_roxburghii Rroxscaffold_2G00095940
rosa_rugosa Rorug02G0430900 Rorug02G0431000
rosa_samantha Rh2DG515100
rosa_wichuraiana Rw2G040410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 440
AccI GTMKAC 1 cut(s) 233
AccII CGCG 2 cut(s) 190, 216
AciI CCGC 9 cut(s) 58, 109, 169, 190, 245, 350, 506, 518, 578
AclWI GGATC 3 cut(s) 566, 590, 791
AcoI YGGCCR 2 cut(s) 191, 246
AcuI CTGAAG 1 cut(s) 248
AcyI GRCGYC 1 cut(s) 236
AfaI GTAC 1 cut(s) 465
AfeI AGCGCT 1 cut(s) 86
AfiI CCNNNNNNNGG 1 cut(s) 806
AflIII ACRYGT 1 cut(s) 214
AgsI TTSAA 3 cut(s) 46, 611, 761
AjnI CCWGG 3 cut(s) 444, 457, 837
AluBI AGCT 4 cut(s) 14, 259, 287, 369
AluI AGCT 4 cut(s) 14, 259, 287, 369
Alw26I GTCTC 1 cut(s) 584
AlwI GGATC 3 cut(s) 566, 590, 791
Ama87I CYCGRG 1 cut(s) 564
Aor51HI AGCGCT 1 cut(s) 86
AoxI GGCC 5 cut(s) 191, 246, 346, 354, 657
ApaI GGGCCC 1 cut(s) 350
ApeKI GCWGC 2 cut(s) 374, 526
Asp700I GAANNNNTTC 1 cut(s) 753
AspLEI GCGC 1 cut(s) 87
AspS9I GGNCC 4 cut(s) 152, 346, 347, 657
AsuC2I CCSGG 2 cut(s) 531, 661
AsuHPI GGTGA 3 cut(s) 28, 553, 821
AsuNHI GCTAGC 1 cut(s) 14
AvaI CYCGRG 1 cut(s) 564
AvaII GGWCC 1 cut(s) 152
BaeGI GKGCMC 2 cut(s) 350, 445
BanI GGYRCC 1 cut(s) 440
BanII GRGCYC 1 cut(s) 350
BauI CACGAG 1 cut(s) 480
BbvCI CCTCAGC 1 cut(s) 260
BbvI GCAGC 2 cut(s) 361, 538
BccI CCATC 5 cut(s) 41, 310, 374, 533, 540
BceAI ACGGC 1 cut(s) 178
BciT130I CCWGG 3 cut(s) 446, 459, 839
BcnI CCSGG 2 cut(s) 531, 661
BcoDI GTCTC 1 cut(s) 584
BfaI CTAG 2 cut(s) 15, 794
BfmI CTRYAG 1 cut(s) 375
BfoI RGCGCY 1 cut(s) 88
BisI GCNGC 5 cut(s) 191, 246, 375, 527, 578
BlsI GCNGC 5 cut(s) 192, 247, 376, 528, 579
Bme1390I CCNGG 5 cut(s) 446, 459, 531, 661, 839
Bme18I GGWCC 1 cut(s) 152
BmeT110I CYCGRG 1 cut(s) 564
BmgT120I GGNCC 4 cut(s) 152, 346, 347, 657
BmiI GGNNCC 3 cut(s) 154, 348, 442
BmrFI CCNGG 5 cut(s) 446, 459, 531, 661, 839
BmrI ACTGGG 1 cut(s) 476
BmsI GCATC 3 cut(s) 169, 180, 232
BmtI GCTAGC 1 cut(s) 18
BmuI ACTGGG 1 cut(s) 476
BoxI GACNNNNGTC 1 cut(s) 828
BplI GAGNNNNNCTC 2 cut(s) 74, 106
Bpu10I CCTNAGC 1 cut(s) 260
BpuEI CTTGAG 1 cut(s) 761
BpuMI CCSGG 2 cut(s) 531, 661
BsaBI GATNNNNATC 1 cut(s) 600
BsaHI GRCGYC 1 cut(s) 236
BsaJI CCNNGG 3 cut(s) 423, 444, 565
BsaXI ACNNNNNCTCC 2 cut(s) 149, 179
Bsc4I CCNNNNNNNGG 1 cut(s) 806
Bse1I ACTGG 1 cut(s) 471
Bse8I GATNNNNATC 1 cut(s) 600
BseBI CCWGG 3 cut(s) 446, 459, 839
BseDI CCNNGG 3 cut(s) 423, 444, 565
BseGI GGATG 3 cut(s) 247, 321, 551
BseJI GATNNNNATC 1 cut(s) 600
BseLI CCNNNNNNNGG 1 cut(s) 806
BseMII CTCAG 2 cut(s) 40, 251
BseNI ACTGG 1 cut(s) 471
BseSI GKGCMC 2 cut(s) 350, 445
BseX3I CGGCCG 1 cut(s) 191
BseXI GCAGC 2 cut(s) 361, 538
BsgI GTGCAG 1 cut(s) 818
Bsh1236I CGCG 2 cut(s) 190, 216
Bsh1285I CGRYCG 1 cut(s) 194
BshFI GGCC 5 cut(s) 193, 248, 348, 356, 659
BshNI GGYRCC 1 cut(s) 440
BsiEI CGRYCG 1 cut(s) 194
BsiHKCI CYCGRG 1 cut(s) 564
BsiSI CCGG 4 cut(s) 239, 412, 531, 660
BslFI GGGAC 1 cut(s) 583
BslI CCNNNNNNNGG 1 cut(s) 806
BsmAI GTCTC 1 cut(s) 584
BsmFI GGGAC 1 cut(s) 583
BsnI GGCC 5 cut(s) 193, 248, 348, 356, 659
BsoBI CYCGRG 1 cut(s) 564
Bsp120I GGGCCC 1 cut(s) 346
Bsp1286I GDGCHC 2 cut(s) 350, 445
Bsp143I GATC 3 cut(s) 558, 595, 796
BspACI CCGC 9 cut(s) 58, 109, 169, 190, 245, 350, 506, 518, 578
BspANI GGCC 5 cut(s) 193, 248, 348, 356, 659
BspCNI CTCAG 2 cut(s) 39, 252
BspFNI CGCG 2 cut(s) 190, 216
BspLI GGNNCC 3 cut(s) 154, 348, 442
BspMAI CTGCAG 1 cut(s) 379
BspOI GCTAGC 1 cut(s) 18
BspPI GGATC 3 cut(s) 566, 590, 791
BspQI GCTCTTC 1 cut(s) 634
BspT107I GGYRCC 1 cut(s) 440
BsrI ACTGG 1 cut(s) 471
BssECI CCNNGG 3 cut(s) 423, 444, 565
BssMI GATC 3 cut(s) 558, 595, 796
BssNI GRCGYC 1 cut(s) 236
BssSI CACGAG 1 cut(s) 480
Bst2BI CACGAG 1 cut(s) 480
Bst2UI CCWGG 3 cut(s) 446, 459, 839
Bst4CI ACNGT 2 cut(s) 268, 574
Bst6I CTCTTC 1 cut(s) 634
BstACI GRCGYC 1 cut(s) 236
BstC8I GCNNGC 4 cut(s) 16, 350, 354, 687
BstDEI CTNAG 2 cut(s) 26, 260
BstENI CCTNNNNNAGG 1 cut(s) 804
BstF5I GGATG 3 cut(s) 247, 321, 551
BstFNI CGCG 2 cut(s) 190, 216
BstH2I RGCGCY 1 cut(s) 88
BstHHI GCGC 1 cut(s) 87
BstKTI GATC 3 cut(s) 561, 598, 799
BstMAI GTCTC 1 cut(s) 584
BstMBI GATC 3 cut(s) 558, 595, 796
BstMCI CGRYCG 1 cut(s) 194
BstMWI GCNNNNNNNGC 1 cut(s) 207
BstNI CCWGG 3 cut(s) 446, 459, 839
BstPAI GACNNNNGTC 1 cut(s) 828
BstSCI CCNGG 5 cut(s) 444, 457, 529, 659, 837
BstSFI CTRYAG 1 cut(s) 375
BstSLI GKGCMC 2 cut(s) 350, 445
BstUI CGCG 2 cut(s) 190, 216
BstV1I GCAGC 2 cut(s) 361, 538
BstX2I RGATCY 2 cut(s) 595, 796
BstYI RGATCY 2 cut(s) 595, 796
BstZI CGGCCG 1 cut(s) 191
BsuRI GGCC 5 cut(s) 193, 248, 348, 356, 659
BtsCI GGATG 3 cut(s) 247, 321, 551
BtsI GCAGTG 1 cut(s) 474
BtsIMutI CAGTG 1 cut(s) 474
Cac8I GCNNGC 4 cut(s) 16, 350, 354, 687
CfoI GCGC 1 cut(s) 87
Cfr13I GGNCC 4 cut(s) 152, 346, 347, 657
CseI GACGC 1 cut(s) 244
Csp6I GTAC 1 cut(s) 464
CviAII CATG 3 cut(s) 67, 731, 788
CviQI GTAC 1 cut(s) 464
DdeI CTNAG 2 cut(s) 26, 260
DpnI GATC 3 cut(s) 560, 597, 798
DpnII GATC 3 cut(s) 558, 595, 796
EaeI YGGCCR 2 cut(s) 191, 246
EagI CGGCCG 1 cut(s) 191
Eam1104I CTCTTC 1 cut(s) 634
EarI CTCTTC 1 cut(s) 634
EclXI CGGCCG 1 cut(s) 191
Eco24I GRGCYC 1 cut(s) 350
Eco47I GGWCC 1 cut(s) 152
Eco47III AGCGCT 1 cut(s) 86
Eco52I CGGCCG 1 cut(s) 191
Eco57I CTGAAG 1 cut(s) 248
Eco88I CYCGRG 1 cut(s) 564
EcoNI CCTNNNNNAGG 1 cut(s) 804
EcoRII CCWGG 3 cut(s) 444, 457, 837
EcoT38I GRGCYC 1 cut(s) 350
FaeI CATG 3 cut(s) 70, 734, 791
FalI AAGNNNNNCTT 2 cut(s) 387, 419
FaqI GGGAC 1 cut(s) 583
FatI CATG 3 cut(s) 66, 730, 787
FauI CCCGC 2 cut(s) 176, 357
FblI GTMKAC 1 cut(s) 233
Fnu4HI GCNGC 5 cut(s) 191, 246, 375, 527, 578
FokI GGATG 3 cut(s) 254, 328, 558
FriOI GRGCYC 1 cut(s) 350
Fsp4HI GCNGC 5 cut(s) 191, 246, 375, 527, 578
FspBI CTAG 2 cut(s) 15, 794
GlaI GCGC 1 cut(s) 86
GluI GCNGC 5 cut(s) 191, 246, 375, 527, 578
HaeII RGCGCY 1 cut(s) 88
HaeIII GGCC 5 cut(s) 193, 248, 348, 356, 659
HapII CCGG 4 cut(s) 239, 412, 531, 660
HgaI GACGC 1 cut(s) 244
HhaI GCGC 1 cut(s) 87
Hin1I GRCGYC 1 cut(s) 236
Hin1II CATG 3 cut(s) 70, 734, 791
Hin6I GCGC 1 cut(s) 85
HinP1I GCGC 1 cut(s) 85
HincII GTYRAC 1 cut(s) 234
HindII GTYRAC 1 cut(s) 234
HpaII CCGG 4 cut(s) 239, 412, 531, 660
HphI GGTGA 3 cut(s) 28, 553, 821
Hpy166II GTNNAC 4 cut(s) 220, 234, 464, 812
Hpy188I TCNGA 6 cut(s) 29, 178, 273, 393, 589, 707
Hpy188III TCNNGA 2 cut(s) 487, 608
Hpy8I GTNNAC 4 cut(s) 220, 234, 464, 812
Hpy99I CGWCG 1 cut(s) 238
HpyAV CCTTC 4 cut(s) 52, 331, 674, 732
HpyCH4III ACNGT 2 cut(s) 268, 574
HpyCH4V TGCA 8 cut(s) 182, 210, 377, 474, 791, 835, 856, 862
HpyF10VI GCNNNNNNNGC 1 cut(s) 207
HpyF3I CTNAG 2 cut(s) 26, 260
Hsp92I GRCGYC 1 cut(s) 236
Hsp92II CATG 3 cut(s) 70, 734, 791
HspAI GCGC 1 cut(s) 85
Kzo9I GATC 3 cut(s) 558, 595, 796
LguI GCTCTTC 1 cut(s) 634
LmnI GCTCC 1 cut(s) 284
Lsp1109I GCAGC 2 cut(s) 361, 538
LweI GCATC 3 cut(s) 169, 180, 232
MaeI CTAG 2 cut(s) 15, 794
MaeIII GTNAC 1 cut(s) 131
MalI GATC 3 cut(s) 560, 597, 798
MboI GATC 3 cut(s) 558, 595, 796
MboII GAAGA 7 cut(s) 16, 19, 425, 651, 710, 749, 758
MfeI CAATTG 1 cut(s) 857
MflI RGATCY 2 cut(s) 595, 796
MhlI GDGCHC 2 cut(s) 350, 445
MluCI AATT 1 cut(s) 857
MluI ACGCGT 1 cut(s) 214
MmeI TCCRAC 1 cut(s) 165
MroXI GAANNNNTTC 1 cut(s) 753
MseI TTAA 1 cut(s) 714
MslI CAYNNNNRTG 1 cut(s) 215
MspA1I CMGCKG 1 cut(s) 111
MspI CCGG 4 cut(s) 239, 412, 531, 660
MspR9I CCNGG 5 cut(s) 446, 459, 531, 661, 839
MunI CAATTG 1 cut(s) 857
MvaI CCWGG 3 cut(s) 446, 459, 839
MvnI CGCG 2 cut(s) 190, 216
MwoI GCNNNNNNNGC 1 cut(s) 207
NciI CCSGG 2 cut(s) 531, 661
NdeII GATC 3 cut(s) 558, 595, 796
NheI GCTAGC 1 cut(s) 14
NlaIII CATG 3 cut(s) 70, 734, 791
NlaIV GGNNCC 3 cut(s) 154, 348, 442
NmeAIII GCCGAG 1 cut(s) 448
PciSI GCTCTTC 1 cut(s) 634
PcsI WCGNNNNNNNCGW 2 cut(s) 123, 230
PdmI GAANNNNTTC 1 cut(s) 753
PkrI GCNGC 5 cut(s) 192, 247, 376, 528, 579
PshAI GACNNNNGTC 1 cut(s) 828
Psp6I CCWGG 3 cut(s) 444, 457, 837
PspGI CCWGG 3 cut(s) 444, 457, 837
PspN4I GGNNCC 3 cut(s) 154, 348, 442
PspOMI GGGCCC 1 cut(s) 346
PspPI GGNCC 4 cut(s) 152, 346, 347, 657
PstI CTGCAG 1 cut(s) 379
PsuI RGATCY 2 cut(s) 595, 796
RsaI GTAC 1 cut(s) 465
RsaNI GTAC 1 cut(s) 464
RseI CAYNNNNRTG 1 cut(s) 215
SalI GTCGAC 1 cut(s) 232
SapI GCTCTTC 1 cut(s) 634
SaqAI TTAA 1 cut(s) 714
SatI GCNGC 5 cut(s) 191, 246, 375, 527, 578
Sau3AI GATC 3 cut(s) 558, 595, 796
Sau96I GGNCC 4 cut(s) 152, 346, 347, 657
ScrFI CCNGG 5 cut(s) 446, 459, 531, 661, 839
SduI GDGCHC 2 cut(s) 350, 445
SfaNI GCATC 3 cut(s) 169, 180, 232
SfcI CTRYAG 1 cut(s) 375
SinI GGWCC 1 cut(s) 152
SmiMI CAYNNNNRTG 1 cut(s) 215
SmlI CTYRAG 1 cut(s) 740
SmoI CTYRAG 1 cut(s) 740
Sse9I AATT 1 cut(s) 857
SsiI CCGC 9 cut(s) 58, 109, 169, 190, 245, 350, 506, 518, 578
SspI AATATT 1 cut(s) 753
SspMI CTAG 2 cut(s) 15, 794
StyD4I CCNGG 5 cut(s) 444, 457, 529, 659, 837
TaaI ACNGT 2 cut(s) 268, 574
TaqI TCGA 4 cut(s) 126, 233, 436, 486
TasI AATT 1 cut(s) 857
TatI WGTACW 1 cut(s) 463
TauI GCSGC 3 cut(s) 193, 248, 580
Tru1I TTAA 1 cut(s) 714
Tru9I TTAA 1 cut(s) 714
TscAI CASTG 1 cut(s) 481
TseI GCWGC 2 cut(s) 374, 526
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 165
TspRI CASTG 1 cut(s) 481
VpaK11BI GGWCC 1 cut(s) 152
XagI CCTNNNNNAGG 1 cut(s) 804
XcmI CCANNNNNNNNNTGG 2 cut(s) 108, 304
XmiI GTMKAC 1 cut(s) 233
XmnI GAANNNNTTC 1 cut(s) 753
XspI CTAG 2 cut(s) 15, 794
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.