pycom09g08480
ERF Family

mTERF

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0006549
Physical Location & Seq
Reverse (-)
6487967 .. 6490590
2624 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g08480.1

Sequence Viewer

Length: 996 bp
ATGTTGGGCTCTGGAGATTCTGCTTTCTGTTGCTTAATTGAATCCTTTCCACGTCTTCTTTTGTTGTCCTTGGATCCTCAAGTGAGACCTTTTGTGGAATATCTTGAAAATATTGGAGTCCCTAGGGAAATAATGAGAAACATATTTCTGTTATTTCCACCTGTTATTTTTTGTAACATTAAAGTCATCAAAACAAGAGTGCTTGCTTTTAAAGAGGTTGGTGTGGAGGAGAAAGATGTCGGCAGAATGTTGATTAAATATCCATGGATTCTATCAATGAGCATTCAAGAGAACTTCAAGGAGGTTTCTTCTTTCTTTGAGTTGGAGAAGGTACCAAAATTGAGTGTTGGCCGTGCAATCAGAAGCTGGCCTCATATTTTGGGTTGCTCAACCAGCATGCTAAAATTGATGGTCGAAGAAATTGGTGAATTGGGTATTAGAAACAAGAAGTTGGATCAGGTTATCTCTAGAAGTCCTCAGCTATTGATACGGAAACCTCAAGAATTTCTTCAGGTAGTTTCATTCGTGGAAGGTCTAGGATTTGATAAAGAAACAGTTGGGAGCATACTGGGGTGTTGTACCAACATATTTGCCGCCAGCATTGGCAAAACTCTCAAGAGGAAGCTGGAATTTCTTGCTAGCATCGGTGTTTCCGAACCCCACCTTCCTCGGGTTATCAAAAAGTATCCAGAGCTTCTCATCTCTGACACTGATAGAACTTTACTTCCTCGGATGAAATATTTAATGAAGAAAGGGCTGTCGTGGAGGGGTATTGCATTCATGGTCCGCAAATTTTCTCCTTTACTCGGGTATAGCATTGAAGATGTTTTGAGTCCGAAGCTTGAATTCCTCATGAACACAATGCAGAAACCAGTGACGGATTTGGTGGAGTATCCAAGGTACTTCAGTTATTCATTGGAAAAGAGGATAAAACCTAGATACTGGGTGCTAAAGGGGAGAAATATCGAGTTTAGCTTACGAGATATGTTGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
mTERF

Protein Analysis

332

Amino Acids

38.1

Weight (kDa)

9.48

Isoelectric Point (pI)

42.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
mTERF PF02536 32 - 331 7e-93 mTERF
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 331
AccB1I GGYRCC 1 cut(s) 331
AciI CCGC 2 cut(s) 594, 787
AclWI GGATC 3 cut(s) 68, 81, 462
AcoI YGGCCR 1 cut(s) 349
AcsI RAATTY 4 cut(s) 503, 629, 791, 845
AcuI CTGAAG 2 cut(s) 494, 889
AfaI GTAC 3 cut(s) 333, 580, 902
AfiI CCNNNNNNNGG 2 cut(s) 670, 806
AgsI TTSAA 6 cut(s) 41, 107, 287, 298, 821, 845
AjiI CACGTC 1 cut(s) 53
AloI GAACNNNNNNTCC 2 cut(s) 709, 741
AluBI AGCT 6 cut(s) 366, 481, 625, 694, 841, 975
AluI AGCT 6 cut(s) 366, 481, 625, 694, 841, 975
Alw26I GTCTC 1 cut(s) 79
AlwI GGATC 3 cut(s) 68, 81, 462
AlwNI CAGNNNCTG 1 cut(s) 366
Ama87I CYCGRG 2 cut(s) 669, 806
AoxI GGCC 2 cut(s) 349, 368
ApoI RAATTY 4 cut(s) 503, 629, 791, 845
Asp700I GAANNNNTTC 2 cut(s) 45, 507
Asp718I GGTACC 1 cut(s) 331
AspA2I CCTAGG 1 cut(s) 122
AspS9I GGNCC 1 cut(s) 784
AsuHPI GGTGA 1 cut(s) 437
AsuNHI GCTAGC 1 cut(s) 638
AvaI CYCGRG 2 cut(s) 669, 806
AvaII GGWCC 1 cut(s) 784
AvrII CCTAGG 1 cut(s) 122
BaeI ACNNNNGTAYC 2 cut(s) 892, 925
BamHI GGATCC 1 cut(s) 73
BanI GGYRCC 1 cut(s) 331
BanII GRGCYC 1 cut(s) 11
BbsI GAAGAC 1 cut(s) 47
BbvCI CCTCAGC 1 cut(s) 477
BccI CCATC 1 cut(s) 403
BceAI ACGGC 1 cut(s) 336
BciVI GTATCC 2 cut(s) 696, 903
BcoDI GTCTC 1 cut(s) 79
BfaI CTAG 5 cut(s) 123, 468, 536, 639, 936
BfuI GTATCC 2 cut(s) 696, 903
BisI GCNGC 1 cut(s) 594
BlnI CCTAGG 1 cut(s) 122
BlsI GCNGC 1 cut(s) 595
Bme18I GGWCC 1 cut(s) 784
BmeT110I CYCGRG 2 cut(s) 669, 806
BmgBI CACGTC 1 cut(s) 53
BmgT120I GGNCC 1 cut(s) 784
BmiI GGNNCC 2 cut(s) 75, 333
BmrI ACTGGG 2 cut(s) 578, 952
BmsI GCATC 1 cut(s) 651
BmtI GCTAGC 1 cut(s) 642
BmuI ACTGGG 2 cut(s) 578, 952
BpiI GAAGAC 1 cut(s) 47
BpmI CTGGAG 1 cut(s) 33
Bpu10I CCTNAGC 1 cut(s) 477
BpuEI CTTGAG 3 cut(s) 63, 483, 599
BsaI GGTCTC 1 cut(s) 79
BsaJI CCNNGG 6 cut(s) 69, 122, 263, 668, 728, 896
Bsc4I CCNNNNNNNGG 2 cut(s) 670, 806
Bse1I ACTGG 3 cut(s) 573, 872, 947
BseDI CCNNGG 6 cut(s) 69, 122, 263, 668, 728, 896
BseGI GGATG 1 cut(s) 738
BseLI CCNNNNNNNGG 2 cut(s) 670, 806
BseMII CTCAG 1 cut(s) 491
BseNI ACTGG 3 cut(s) 573, 872, 947
BseRI GAGGAG 1 cut(s) 242
BshFI GGCC 2 cut(s) 351, 370
BshNI GGYRCC 1 cut(s) 331
BsiHKCI CYCGRG 2 cut(s) 669, 806
BslFI GGGAC 1 cut(s) 104
BslI CCNNNNNNNGG 2 cut(s) 670, 806
BsmAI GTCTC 1 cut(s) 79
BsmFI GGGAC 1 cut(s) 104
BsmI GAATGC 2 cut(s) 282, 776
BsnI GGCC 2 cut(s) 351, 370
Bso31I GGTCTC 1 cut(s) 79
BsoBI CYCGRG 2 cut(s) 669, 806
Bsp1286I GDGCHC 1 cut(s) 11
Bsp143I GATC 2 cut(s) 73, 454
Bsp19I CCATGG 1 cut(s) 263
BspACI CCGC 2 cut(s) 594, 787
BspANI GGCC 2 cut(s) 351, 370
BspCNI CTCAG 1 cut(s) 490
BspHI TCATGA 1 cut(s) 852
BspLI GGNNCC 2 cut(s) 75, 333
BspOI GCTAGC 1 cut(s) 642
BspPI GGATC 3 cut(s) 68, 81, 462
BspT107I GGYRCC 1 cut(s) 331
BspTNI GGTCTC 1 cut(s) 79
BsrI ACTGG 3 cut(s) 573, 872, 947
BssECI CCNNGG 6 cut(s) 69, 122, 263, 668, 728, 896
BssMI GATC 2 cut(s) 73, 454
BssT1I CCWWGG 4 cut(s) 69, 122, 263, 896
Bst4CI ACNGT 1 cut(s) 556
BstC8I GCNNGC 5 cut(s) 204, 368, 398, 598, 640
BstDEI CTNAG 1 cut(s) 477
BstDSI CCRYGG 1 cut(s) 263
BstF5I GGATG 1 cut(s) 738
BstKTI GATC 2 cut(s) 76, 457
BstMAI GTCTC 1 cut(s) 79
BstMBI GATC 2 cut(s) 73, 454
BstMWI GCNNNNNNNGC 1 cut(s) 393
BstNSI RCATGY 1 cut(s) 400
BstV2I GAAGAC 1 cut(s) 47
BstX2I RGATCY 1 cut(s) 73
BstYI RGATCY 1 cut(s) 73
BsuI GTATCC 2 cut(s) 696, 903
BsuRI GGCC 2 cut(s) 351, 370
BtgI CCRYGG 1 cut(s) 263
BtrI CACGTC 1 cut(s) 53
BtsCI GGATG 1 cut(s) 738
BtsIMutI CAGTG 2 cut(s) 708, 879
Cac8I GCNNGC 5 cut(s) 204, 368, 398, 598, 640
CaiI CAGNNNCTG 1 cut(s) 366
CciI TCATGA 1 cut(s) 852
Cfr13I GGNCC 1 cut(s) 784
Csp6I GTAC 3 cut(s) 332, 579, 901
CviAII CATG 4 cut(s) 264, 397, 781, 853
CviQI GTAC 3 cut(s) 332, 579, 901
DdeI CTNAG 1 cut(s) 477
DpnI GATC 2 cut(s) 75, 456
DpnII GATC 2 cut(s) 73, 454
DraI TTTAAA 1 cut(s) 211
EaeI YGGCCR 1 cut(s) 349
Eco130I CCWWGG 4 cut(s) 69, 122, 263, 896
Eco24I GRGCYC 1 cut(s) 11
Eco31I GGTCTC 1 cut(s) 79
Eco47I GGWCC 1 cut(s) 784
Eco57I CTGAAG 2 cut(s) 494, 889
Eco88I CYCGRG 2 cut(s) 669, 806
EcoRI GAATTC 1 cut(s) 845
EcoT14I CCWWGG 4 cut(s) 69, 122, 263, 896
EcoT38I GRGCYC 1 cut(s) 11
ErhI CCWWGG 4 cut(s) 69, 122, 263, 896
FaeI CATG 4 cut(s) 267, 400, 784, 856
FalI AAGNNNNNCTT 2 cut(s) 492, 524
FaqI GGGAC 1 cut(s) 104
FatI CATG 4 cut(s) 263, 396, 780, 852
Fnu4HI GCNGC 1 cut(s) 594
FokI GGATG 1 cut(s) 745
FriOI GRGCYC 1 cut(s) 11
Fsp4HI GCNGC 1 cut(s) 594
FspBI CTAG 5 cut(s) 123, 468, 536, 639, 936
GluI GCNGC 1 cut(s) 594
GsuI CTGGAG 1 cut(s) 33
HaeIII GGCC 2 cut(s) 351, 370
Hin1II CATG 4 cut(s) 267, 400, 784, 856
HindIII AAGCTT 1 cut(s) 839
HinfI GANTC 5 cut(s) 17, 41, 117, 268, 832
HphI GGTGA 1 cut(s) 437
Hpy188I TCNGA 5 cut(s) 362, 655, 706, 732, 837
Hpy188III TCNNGA 8 cut(s) 12, 104, 287, 468, 500, 616, 689, 853
HpyAV CCTTC 3 cut(s) 322, 524, 674
HpyCH4III ACNGT 1 cut(s) 556
HpyCH4IV ACGT 1 cut(s) 52
HpyCH4V TGCA 3 cut(s) 356, 776, 865
HpyF10VI GCNNNNNNNGC 1 cut(s) 393
HpyF3I CTNAG 1 cut(s) 477
HpySE526I ACGT 1 cut(s) 52
Hsp92II CATG 4 cut(s) 267, 400, 784, 856
KpnI GGTACC 1 cut(s) 335
Kzo9I GATC 2 cut(s) 73, 454
LmnI GCTCC 1 cut(s) 561
LweI GCATC 1 cut(s) 651
MaeI CTAG 5 cut(s) 123, 468, 536, 639, 936
MaeII ACGT 1 cut(s) 52
MaeIII GTNAC 2 cut(s) 173, 874
MalI GATC 2 cut(s) 75, 456
MboI GATC 2 cut(s) 73, 454
MboII GAAGA 6 cut(s) 47, 300, 428, 500, 760, 833
MflI RGATCY 1 cut(s) 73
MhlI GDGCHC 1 cut(s) 11
MluCI AATT 9 cut(s) 36, 338, 404, 420, 428, 503, 629, 791, 845
MlyI GAGTC 2 cut(s) 126, 841
MmeI TCCRAC 2 cut(s) 303, 432
MroXI GAANNNNTTC 2 cut(s) 45, 507
MseI TTAA 5 cut(s) 35, 180, 210, 255, 743
Mva1269I GAATGC 2 cut(s) 282, 776
MwoI GCNNNNNNNGC 1 cut(s) 393
NcoI CCATGG 1 cut(s) 263
NdeII GATC 2 cut(s) 73, 454
NheI GCTAGC 1 cut(s) 638
NlaIII CATG 4 cut(s) 267, 400, 784, 856
NlaIV GGNNCC 2 cut(s) 75, 333
NmuCI GTSAC 1 cut(s) 874
NspI RCATGY 1 cut(s) 400
PaeI GCATGC 1 cut(s) 400
PagI TCATGA 1 cut(s) 852
PcsI WCGNNNNNNNCGW 1 cut(s) 651
PctI GAATGC 2 cut(s) 282, 776
PdmI GAANNNNTTC 2 cut(s) 45, 507
PfeI GAWTC 3 cut(s) 17, 41, 268
PkrI GCNGC 1 cut(s) 595
PleI GAGTC 2 cut(s) 125, 840
PpsI GAGTC 2 cut(s) 125, 840
PspN4I GGNNCC 2 cut(s) 75, 333
PspPI GGNCC 1 cut(s) 784
PstNI CAGNNNCTG 1 cut(s) 366
PsuI RGATCY 1 cut(s) 73
RsaI GTAC 3 cut(s) 333, 580, 902
RsaNI GTAC 3 cut(s) 332, 579, 901
SaqAI TTAA 5 cut(s) 35, 180, 210, 255, 743
SatI GCNGC 1 cut(s) 594
Sau3AI GATC 2 cut(s) 73, 454
Sau96I GGNCC 1 cut(s) 784
SchI GAGTC 2 cut(s) 126, 841
SduI GDGCHC 1 cut(s) 11
SfaNI GCATC 1 cut(s) 651
SinI GGWCC 1 cut(s) 784
SmlI CTYRAG 3 cut(s) 78, 498, 614
SmoI CTYRAG 3 cut(s) 78, 498, 614
SphI GCATGC 1 cut(s) 400
Sse9I AATT 9 cut(s) 36, 338, 404, 420, 428, 503, 629, 791, 845
SsiI CCGC 2 cut(s) 594, 787
SspI AATATT 2 cut(s) 112, 740
SspMI CTAG 5 cut(s) 123, 468, 536, 639, 936
StyI CCWWGG 4 cut(s) 69, 122, 263, 896
TaaI ACNGT 1 cut(s) 556
TaiI ACGT 1 cut(s) 55
TaqI TCGA 2 cut(s) 414, 966
TasI AATT 9 cut(s) 36, 338, 404, 420, 428, 503, 629, 791, 845
TauI GCSGC 1 cut(s) 596
TfiI GAWTC 3 cut(s) 17, 41, 268
Tru1I TTAA 5 cut(s) 35, 180, 210, 255, 743
Tru9I TTAA 5 cut(s) 35, 180, 210, 255, 743
TscAI CASTG 2 cut(s) 715, 879
TseFI GTSAC 1 cut(s) 874
Tsp45I GTSAC 1 cut(s) 874
TspDTI ATGAA 6 cut(s) 510, 749, 761, 769, 869, 903
TspGWI ACGGA 2 cut(s) 505, 893
TspRI CASTG 2 cut(s) 715, 879
VpaK11BI GGWCC 1 cut(s) 784
XapI RAATTY 4 cut(s) 503, 629, 791, 845
XbaI TCTAGA 1 cut(s) 467
XceI RCATGY 1 cut(s) 400
XmaJI CCTAGG 1 cut(s) 122
XmnI GAANNNNTTC 2 cut(s) 45, 507
XspI CTAG 5 cut(s) 123, 468, 536, 639, 936
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.