pycom09g08910

Mitochondrial K+-H+ exchange-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0011394
Physical Location & Seq
Reverse (-)
7056884 .. 7059583
2700 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 840 bp
ATGAGAGCGAGAGTGATAGTTTTTCCGGTTAAAGGGAGGAACTGGTGCTTCAGCAGATCCATTGAGCCTCTGGTTTCAGACTCTGCATCTGCCTCTCAAACTCCTTCAACTCTCAAAGATCTCTGGAAAAAGTACAAGTACAATGCCAATGACAAGGCCATTGCCAATCCTTTAGCTGCCAACGCTGAGCTCCTCATTGATTTCGTCTCTACCAAGATGAACAGAGCTTGGATCGGTCTGGAGAAGTCGCCTCAGGGAAGTTTTAAGAACAAGCTTCATGGATTGGGATTGAAGTTGTTGGCTCGTGTTAAGCCGTCTGAGATTTTCCTGAAATCGATCTCTACTGAGGTCACAGGTGTTGAAGTTAAATACCCGTCCAGCTTAAATGCACGACTTGTGCGTAGGAGGCTACGGCATATTGCCATGAGGGGATCTGTCATACACAAAAAGTATTTATATGGTTCAGTTACGTTGCTTCCTCTGACATCTGCATTTACTGTTTTGCCTTTGCCTAATATTCCCTTCTTCTGGTGTTTATTCCGTACTTATTCTCATTGGCGAGCCCTCAAGGGAAGCGAGAAGCTCCTTCAGCTAGTCTCAGATAGCTCTGTGACGCCAAGTCACACTACTGATGGAAATGAAAATGAGCATATTGACTCTCAGCATGGAAGCAAAGAGACATTTGATTCTCCTTACGTTTTGCAGCCATCGAAAGCGCTTGAGGAACTTCTTCGTTATGGAGGCGAGCGTGAGGGTCTCAAAGAATGTGCGATATTAGATATCTGCAAAACCTTCGATTTGAACACGAATGATGTTTTAAAGTACAGGGGCTCGACGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mit_KHE1

Protein Analysis

280

Amino Acids

31.42

Weight (kDa)

9.64

Isoelectric Point (pI)

46.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mit_KHE1 PF10173 4 - 199 2.1e-42 Mitochondrial K+-H+ exchange-related
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011394)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53760 AT1G53760
fragaria_vesca FvH4_6g36070 FvH4_6g36070
malus_domestica MD09G1171800.v1.1 MD17G1155200.v1.1
prunus_persica Prupe.3G030200_v2.0.a1
pyrus_communis pycom09g08910 pycom17g14800
rosa_chinensis RchiOBHm_Chr2g0148141
rosa_laevigata RLG00000020320
rosa_multiflora Rmu_sc0001516.1_g000097 Rmu_sc0012028.1_g000004
rosa_roxburghii Rroxscaffold_2G00099050
rosa_rugosa Rorug02G0403900
rosa_samantha Rh2AG462500 Rh2BG475500 Rh2CG449300 Rh2DG484200
rosa_wichuraiana Rw2G037700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 51, 239, 439
AcuI CTGAAG 2 cut(s) 34, 572
AcyI GRCGYC 1 cut(s) 614
AfaI GTAC 4 cut(s) 134, 140, 544, 824
AfeI AGCGCT 1 cut(s) 717
AfiI CCNNNNNNNGG 2 cut(s) 32, 528
AgsI TTSAA 4 cut(s) 108, 292, 362, 802
AhdI GACNNNNNGTC 1 cut(s) 618
AluBI AGCT 8 cut(s) 176, 190, 227, 274, 381, 583, 592, 606
AluI AGCT 8 cut(s) 176, 190, 227, 274, 381, 583, 592, 606
Alw21I GWGCWC 1 cut(s) 192
Alw26I GTCTC 4 cut(s) 211, 601, 671, 761
AlwI GGATC 3 cut(s) 51, 239, 439
AlwNI CAGNNNCTG 1 cut(s) 83
Aor51HI AGCGCT 1 cut(s) 717
AoxI GGCC 1 cut(s) 156
ApeKI GCWGC 2 cut(s) 176, 703
Asp700I GAANNNNTTC 1 cut(s) 729
AspLEI GCGC 1 cut(s) 718
AxyI CCTNAGG 1 cut(s) 252
BanII GRGCYC 3 cut(s) 192, 565, 833
BauI CACGAG 1 cut(s) 303
Bbv12I GWGCWC 1 cut(s) 192
BbvI GCAGC 2 cut(s) 163, 715
BccI CCATC 2 cut(s) 626, 715
BceAI ACGGC 2 cut(s) 298, 428
BcgI CGANNNNNNTGC 2 cut(s) 775, 809
BcoDI GTCTC 4 cut(s) 211, 601, 671, 761
BfaI CTAG 1 cut(s) 593
BfoI RGCGCY 1 cut(s) 719
BglII AGATCT 1 cut(s) 118
BisI GCNGC 2 cut(s) 177, 704
BlpI GCTNAGC 1 cut(s) 186
BlsI GCNGC 2 cut(s) 178, 705
BmeRI GACNNNNNGTC 1 cut(s) 618
BmsI GCATC 1 cut(s) 95
BpmI CTGGAG 1 cut(s) 260
Bpu1102I GCTNAGC 1 cut(s) 186
BpuEI CTTGAG 2 cut(s) 551, 740
Bsa29I ATCGAT 1 cut(s) 335
BsaHI GRCGYC 1 cut(s) 614
BsaI GGTCTC 1 cut(s) 761
BsaWI WCCGGW 1 cut(s) 25
Bsc4I CCNNNNNNNGG 2 cut(s) 32, 528
Bse1I ACTGG 1 cut(s) 47
Bse21I CCTNAGG 1 cut(s) 252
Bse3DI GCAATG 1 cut(s) 159
BseCI ATCGAT 1 cut(s) 335
BseLI CCNNNNNNNGG 2 cut(s) 32, 528
BseMI GCAATG 1 cut(s) 159
BseMII CTCAG 6 cut(s) 177, 266, 309, 336, 612, 674
BseNI ACTGG 1 cut(s) 47
BseRI GAGGAG 1 cut(s) 182
BseXI GCAGC 2 cut(s) 163, 715
BshFI GGCC 1 cut(s) 158
BshVI ATCGAT 1 cut(s) 335
BsiHKAI GWGCWC 1 cut(s) 192
BsiSI CCGG 1 cut(s) 26
BslI CCNNNNNNNGG 2 cut(s) 32, 528
BsmAI GTCTC 4 cut(s) 211, 601, 671, 761
BsmBI CGTCTC 1 cut(s) 211
BsnI GGCC 1 cut(s) 158
Bso31I GGTCTC 1 cut(s) 761
Bsp1286I GDGCHC 3 cut(s) 192, 565, 833
Bsp143I GATC 5 cut(s) 56, 118, 231, 336, 431
Bsp1720I GCTNAGC 1 cut(s) 186
BspANI GGCC 1 cut(s) 158
BspCNI CTCAG 6 cut(s) 178, 265, 310, 337, 611, 673
BspDI ATCGAT 1 cut(s) 335
BspPI GGATC 3 cut(s) 51, 239, 439
BspTNI GGTCTC 1 cut(s) 761
BsrDI GCAATG 1 cut(s) 159
BsrI ACTGG 1 cut(s) 47
BssMI GATC 5 cut(s) 56, 118, 231, 336, 431
BssNI GRCGYC 1 cut(s) 614
BssSI CACGAG 1 cut(s) 303
Bst2BI CACGAG 1 cut(s) 303
Bst4CI ACNGT 1 cut(s) 499
BstACI GRCGYC 1 cut(s) 614
BstC8I GCNNGC 2 cut(s) 561, 746
BstDEI CTNAG 6 cut(s) 186, 252, 318, 345, 598, 660
BstH2I RGCGCY 1 cut(s) 719
BstHHI GCGC 1 cut(s) 718
BstKTI GATC 5 cut(s) 59, 121, 234, 339, 434
BstMAI GTCTC 4 cut(s) 211, 601, 671, 761
BstMBI GATC 5 cut(s) 56, 118, 231, 336, 431
BstMWI GCNNNNNNNGC 3 cut(s) 182, 406, 589
BstV1I GCAGC 2 cut(s) 163, 715
BstX2I RGATCY 3 cut(s) 56, 118, 431
BstYI RGATCY 3 cut(s) 56, 118, 431
Bsu15I ATCGAT 1 cut(s) 335
Bsu36I CCTNAGG 1 cut(s) 252
BsuRI GGCC 1 cut(s) 158
BsuTUI ATCGAT 1 cut(s) 335
Cac8I GCNNGC 2 cut(s) 561, 746
CaiI CAGNNNCTG 1 cut(s) 83
CfoI GCGC 1 cut(s) 718
ClaI ATCGAT 1 cut(s) 335
CseI GACGC 1 cut(s) 622
Csp6I GTAC 4 cut(s) 133, 139, 543, 823
CviAII CATG 3 cut(s) 278, 424, 665
CviQI GTAC 4 cut(s) 133, 139, 543, 823
DdeI CTNAG 6 cut(s) 186, 252, 318, 345, 598, 660
DpnI GATC 5 cut(s) 58, 120, 233, 338, 433
DpnII GATC 5 cut(s) 56, 118, 231, 336, 431
DraI TTTAAA 1 cut(s) 819
DriI GACNNNNNGTC 1 cut(s) 618
Eam1105I GACNNNNNGTC 1 cut(s) 618
Ecl136II GAGCTC 1 cut(s) 190
Eco24I GRGCYC 3 cut(s) 192, 565, 833
Eco31I GGTCTC 1 cut(s) 761
Eco32I GATATC 1 cut(s) 781
Eco47III AGCGCT 1 cut(s) 717
Eco53kI GAGCTC 1 cut(s) 190
Eco57I CTGAAG 2 cut(s) 34, 572
Eco81I CCTNAGG 1 cut(s) 252
EcoICRI GAGCTC 1 cut(s) 190
EcoRV GATATC 1 cut(s) 781
EcoT38I GRGCYC 3 cut(s) 192, 565, 833
Esp3I CGTCTC 1 cut(s) 211
FaeI CATG 3 cut(s) 281, 427, 668
FaiI YATR 9 cut(s) 279, 417, 425, 440, 457, 459, 651, 666, 738
FatI CATG 3 cut(s) 277, 423, 664
Fnu4HI GCNGC 2 cut(s) 177, 704
FriOI GRGCYC 3 cut(s) 192, 565, 833
Fsp4HI GCNGC 2 cut(s) 177, 704
FspBI CTAG 1 cut(s) 593
GlaI GCGC 1 cut(s) 717
GluI GCNGC 2 cut(s) 177, 704
GsuI CTGGAG 1 cut(s) 260
HaeII RGCGCY 1 cut(s) 719
HaeIII GGCC 1 cut(s) 158
HapII CCGG 1 cut(s) 26
HgaI GACGC 1 cut(s) 622
HhaI GCGC 1 cut(s) 718
Hin1I GRCGYC 1 cut(s) 614
Hin1II CATG 3 cut(s) 281, 427, 668
Hin6I GCGC 1 cut(s) 716
HinP1I GCGC 1 cut(s) 716
HindIII AAGCTT 1 cut(s) 272
HinfI GANTC 3 cut(s) 80, 656, 686
HpaII CCGG 1 cut(s) 26
Hpy188I TCNGA 4 cut(s) 79, 319, 483, 601
Hpy188III TCNNGA 3 cut(s) 124, 239, 328
Hpy99I CGWCG 1 cut(s) 838
HpyAV CCTTC 4 cut(s) 114, 532, 596, 802
HpyCH4III ACNGT 1 cut(s) 499
HpyCH4IV ACGT 3 cut(s) 470, 696, 836
HpyCH4V TGCA 5 cut(s) 86, 389, 491, 703, 786
HpyF10VI GCNNNNNNNGC 3 cut(s) 182, 406, 589
HpyF3I CTNAG 6 cut(s) 186, 252, 318, 345, 598, 660
HpySE526I ACGT 3 cut(s) 470, 696, 836
Hsp92I GRCGYC 1 cut(s) 614
Hsp92II CATG 3 cut(s) 281, 427, 668
HspAI GCGC 1 cut(s) 716
Kzo9I GATC 5 cut(s) 56, 118, 231, 336, 431
LmnI GCTCC 2 cut(s) 195, 588
Lsp1109I GCAGC 2 cut(s) 163, 715
LweI GCATC 1 cut(s) 95
MaeI CTAG 1 cut(s) 593
MaeII ACGT 3 cut(s) 470, 696, 836
MaeIII GTNAC 4 cut(s) 349, 466, 610, 620
MalI GATC 5 cut(s) 58, 120, 233, 338, 433
MboI GATC 5 cut(s) 56, 118, 231, 336, 431
MboII GAAGA 2 cut(s) 517, 722
MflI RGATCY 3 cut(s) 56, 118, 431
MhlI GDGCHC 3 cut(s) 192, 565, 833
MlyI GAGTC 2 cut(s) 74, 650
MroXI GAANNNNTTC 1 cut(s) 729
MseI TTAA 6 cut(s) 30, 264, 309, 366, 383, 818
MspI CCGG 1 cut(s) 26
MwoI GCNNNNNNNGC 3 cut(s) 182, 406, 589
NdeII GATC 5 cut(s) 56, 118, 231, 336, 431
NlaIII CATG 3 cut(s) 281, 427, 668
NmuCI GTSAC 3 cut(s) 349, 610, 620
PcsI WCGNNNNNNNCGW 1 cut(s) 397
PdmI GAANNNNTTC 1 cut(s) 729
PfeI GAWTC 1 cut(s) 686
PkrI GCNGC 2 cut(s) 178, 705
PleI GAGTC 2 cut(s) 74, 650
PpsI GAGTC 2 cut(s) 74, 650
Psp124BI GAGCTC 1 cut(s) 192
PstNI CAGNNNCTG 1 cut(s) 83
PsuI RGATCY 3 cut(s) 56, 118, 431
RsaI GTAC 4 cut(s) 134, 140, 544, 824
RsaNI GTAC 4 cut(s) 133, 139, 543, 823
SacI GAGCTC 1 cut(s) 192
SaqAI TTAA 6 cut(s) 30, 264, 309, 366, 383, 818
SatI GCNGC 2 cut(s) 177, 704
Sau3AI GATC 5 cut(s) 56, 118, 231, 336, 431
SchI GAGTC 2 cut(s) 74, 650
SduI GDGCHC 3 cut(s) 192, 565, 833
SfaNI GCATC 1 cut(s) 95
SmlI CTYRAG 2 cut(s) 566, 719
SmoI CTYRAG 2 cut(s) 566, 719
SspI AATATT 1 cut(s) 517
SspMI CTAG 1 cut(s) 593
SstI GAGCTC 1 cut(s) 192
TaaI ACNGT 1 cut(s) 499
TaiI ACGT 3 cut(s) 473, 699, 839
TaqI TCGA 4 cut(s) 335, 710, 795, 833
TaqII GACCGA 1 cut(s) 224
TatI WGTACW 3 cut(s) 132, 138, 822
TfiI GAWTC 1 cut(s) 686
Tru1I TTAA 6 cut(s) 30, 264, 309, 366, 383, 818
Tru9I TTAA 6 cut(s) 30, 264, 309, 366, 383, 818
TseFI GTSAC 3 cut(s) 349, 610, 620
TseI GCWGC 2 cut(s) 176, 703
Tsp45I GTSAC 3 cut(s) 349, 610, 620
TspDTI ATGAA 3 cut(s) 233, 266, 654
TspGWI ACGGA 1 cut(s) 530
XcmI CCANNNNNNNNNTGG 1 cut(s) 67
XmnI GAANNNNTTC 1 cut(s) 729
XspI CTAG 1 cut(s) 593
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.