pycom09g09470

DnaJ homolog subfamily B member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0010346
Physical Location & Seq
Forward (+)
7643465 .. 7647706
4242 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 711 bp
ATGGCGGATGGGGAAGAGAAAGGCAGTGACTTCTATGCAGTTTTGGGGTTGGAAAAGGAATGCACAGACTCGGAGCTCAGAAATGCCTATAAGAAACTTGCACTGAGATGGCACCCGGATCGGTGTTCGGCCTCCGGAAATTCAAAGTTCGTGGAAGAAGCCAAGAAGAAATTCCAGGACATCCAAGAAGCCTATTCTGTTTTATCTGACGCGAATAAGAGGTTTCTGTACGATGTTGGGGCCTATGGCAGTGATGATGACGAAAACATTGGGATGGGTGAGTTTTTGGACGAGATGGCAGTGATGATGCGCCAAACGAAGCCTAATGAGACCTTTGAAGAATTGAAAAATCTTTTTGATGAAATGTTTGAAGGGGATACTGGGAGTTATGGGTCAGTCTCTCACCCTCCTAGTTCCTGTTCTACGTCTTCGTATGTGTCCTATTGTGAAAGATCAAGTTCCAGTACTAAGCGGAATTGCTCCGAAATGAACTATGAGAAGGCGACCTTGGACGATCCTTCTGGCTTCGACACTCATTTTCAGAGCTTTTGTTTAGGGGTGGAAAGCCTTCAAGACATCGGGGAGGGGACGGCAGCAAGAGAAGTGATTCGCGCAGAAGCCATCGGTAATCGAAGGCAGGGCAGGAAACAAAAGGTTACATCTGGTAATGATGTTTCTTCAAACGACTACTCTGGTATATCAGCTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0051087
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

237

Amino Acids

26.24

Weight (kDa)

4.74

Isoelectric Point (pI)

49.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 10 - 78 3.9e-21 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 111
AccII CGCG 2 cut(s) 212, 612
AccIII TCCGGA 1 cut(s) 134
AciI CCGC 2 cut(s) 5, 472
AclWI GGATC 2 cut(s) 126, 509
AcsI RAATTY 2 cut(s) 139, 170
AfaI GTAC 2 cut(s) 230, 466
AgsI TTSAA 6 cut(s) 144, 338, 346, 371, 572, 681
AjnI CCWGG 1 cut(s) 174
AjuI GAANNNNNNNTTGG 4 cut(s) 155, 187, 491, 523
AluBI AGCT 3 cut(s) 76, 546, 704
AluI AGCT 3 cut(s) 76, 546, 704
Alw21I GWGCWC 1 cut(s) 78
Alw26I GTCTC 2 cut(s) 323, 403
AlwI GGATC 2 cut(s) 126, 509
Aor13HI TCCGGA 1 cut(s) 134
AoxI GGCC 2 cut(s) 129, 240
ApeKI GCWGC 1 cut(s) 593
ApoI RAATTY 2 cut(s) 139, 170
Asp700I GAANNNNTTC 3 cut(s) 170, 567, 606
AspLEI GCGC 2 cut(s) 312, 614
AspS9I GGNCC 1 cut(s) 240
AsuC2I CCSGG 1 cut(s) 116
AsuHPI GGTGA 2 cut(s) 290, 395
BanI GGYRCC 1 cut(s) 111
BanII GRGCYC 1 cut(s) 78
BbsI GAAGAC 1 cut(s) 420
Bbv12I GWGCWC 1 cut(s) 78
BbvI GCAGC 1 cut(s) 605
BccI CCATC 5 cut(s) 2, 102, 268, 289, 629
BceAI ACGGC 1 cut(s) 606
BcgI CGANNNNNNTGC 2 cut(s) 101, 135
BciT130I CCWGG 1 cut(s) 176
BciVI GTATCC 1 cut(s) 370
BcnI CCSGG 1 cut(s) 116
BcoDI GTCTC 2 cut(s) 323, 403
BfaI CTAG 2 cut(s) 411, 705
BfuI GTATCC 1 cut(s) 370
BisI GCNGC 1 cut(s) 594
BlsI GCNGC 1 cut(s) 595
BmcAI AGTACT 1 cut(s) 466
Bme1390I CCNGG 2 cut(s) 116, 176
BmgT120I GGNCC 1 cut(s) 240
BmiI GGNNCC 2 cut(s) 113, 241
BmrFI CCNGG 2 cut(s) 116, 176
BmrI ACTGGG 1 cut(s) 390
BmsI GCATC 1 cut(s) 297
BmuI ACTGGG 1 cut(s) 390
BpiI GAAGAC 1 cut(s) 420
BpuMI CCSGG 1 cut(s) 116
BsaI GGTCTC 1 cut(s) 323
BsaJI CCNNGG 1 cut(s) 507
BsaWI WCCGGW 1 cut(s) 134
Bse1I ACTGG 2 cut(s) 385, 462
BseAI TCCGGA 1 cut(s) 134
BseBI CCWGG 1 cut(s) 176
BseDI CCNNGG 1 cut(s) 507
BseGI GGATG 3 cut(s) 13, 180, 279
BseMII CTCAG 2 cut(s) 91, 95
BseNI ACTGG 2 cut(s) 385, 462
BseXI GCAGC 1 cut(s) 605
Bsh1236I CGCG 2 cut(s) 212, 612
BshFI GGCC 2 cut(s) 131, 242
BshNI GGYRCC 1 cut(s) 111
BsiHKAI GWGCWC 1 cut(s) 78
BsiSI CCGG 2 cut(s) 116, 135
BslFI GGGAC 1 cut(s) 601
BsmAI GTCTC 2 cut(s) 323, 403
BsmFI GGGAC 1 cut(s) 601
BsmI GAATGC 1 cut(s) 65
BsnI GGCC 2 cut(s) 131, 242
Bso31I GGTCTC 1 cut(s) 323
Bsp1286I GDGCHC 1 cut(s) 78
Bsp13I TCCGGA 1 cut(s) 134
Bsp143I GATC 3 cut(s) 118, 452, 514
BspACI CCGC 2 cut(s) 5, 472
BspANI GGCC 2 cut(s) 131, 242
BspCNI CTCAG 2 cut(s) 90, 96
BspEI TCCGGA 1 cut(s) 134
BspFNI CGCG 2 cut(s) 212, 612
BspLI GGNNCC 2 cut(s) 113, 241
BspPI GGATC 2 cut(s) 126, 509
BspT107I GGYRCC 1 cut(s) 111
BspTNI GGTCTC 1 cut(s) 323
BsrI ACTGG 2 cut(s) 385, 462
BssECI CCNNGG 1 cut(s) 507
BssMI GATC 3 cut(s) 118, 452, 514
BssT1I CCWWGG 1 cut(s) 507
Bst2UI CCWGG 1 cut(s) 176
Bst6I CTCTTC 1 cut(s) 9
BstDEI CTNAG 3 cut(s) 77, 104, 468
BstF5I GGATG 3 cut(s) 13, 180, 279
BstFNI CGCG 2 cut(s) 212, 612
BstHHI GCGC 2 cut(s) 312, 614
BstKTI GATC 3 cut(s) 121, 455, 517
BstMAI GTCTC 2 cut(s) 323, 403
BstMBI GATC 3 cut(s) 118, 452, 514
BstNI CCWGG 1 cut(s) 176
BstSCI CCNGG 2 cut(s) 114, 174
BstUI CGCG 2 cut(s) 212, 612
BstV1I GCAGC 1 cut(s) 605
BstV2I GAAGAC 1 cut(s) 420
BsuI GTATCC 1 cut(s) 370
BsuRI GGCC 2 cut(s) 131, 242
BtsCI GGATG 3 cut(s) 13, 180, 279
BtsI GCAGTG 3 cut(s) 31, 256, 306
BtsIMutI CAGTG 4 cut(s) 31, 101, 256, 306
CfoI GCGC 2 cut(s) 312, 614
Cfr13I GGNCC 1 cut(s) 240
CseI GACGC 1 cut(s) 218
Csp6I GTAC 2 cut(s) 229, 465
CspCI CAANNNNNGTGG 2 cut(s) 132, 167
CviQI GTAC 2 cut(s) 229, 465
DdeI CTNAG 3 cut(s) 77, 104, 468
DpnI GATC 3 cut(s) 120, 454, 516
DpnII GATC 3 cut(s) 118, 452, 514
Eam1104I CTCTTC 1 cut(s) 9
EarI CTCTTC 1 cut(s) 9
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 76
Eco130I CCWWGG 1 cut(s) 507
Eco24I GRGCYC 1 cut(s) 78
Eco31I GGTCTC 1 cut(s) 323
Eco53kI GAGCTC 1 cut(s) 76
EcoICRI GAGCTC 1 cut(s) 76
EcoO109I RGGNCCY 1 cut(s) 240
EcoRII CCWGG 1 cut(s) 174
EcoT14I CCWWGG 1 cut(s) 507
EcoT38I GRGCYC 1 cut(s) 78
ErhI CCWWGG 1 cut(s) 507
FaiI YATR 7 cut(s) 36, 90, 246, 390, 435, 495, 698
FalI AAGNNNNNCTT 2 cut(s) 491, 523
FaqI GGGAC 1 cut(s) 601
Fnu4HI GCNGC 1 cut(s) 594
FokI GGATG 3 cut(s) 20, 167, 286
FriOI GRGCYC 1 cut(s) 78
Fsp4HI GCNGC 1 cut(s) 594
FspBI CTAG 2 cut(s) 411, 705
GlaI GCGC 2 cut(s) 311, 613
GluI GCNGC 1 cut(s) 594
HaeIII GGCC 2 cut(s) 131, 242
HapII CCGG 2 cut(s) 116, 135
HgaI GACGC 1 cut(s) 218
HhaI GCGC 2 cut(s) 312, 614
Hin6I GCGC 2 cut(s) 310, 612
HinP1I GCGC 2 cut(s) 310, 612
HinfI GANTC 2 cut(s) 68, 607
HpaII CCGG 2 cut(s) 116, 135
HphI GGTGA 2 cut(s) 290, 395
Hpy188I TCNGA 5 cut(s) 73, 80, 208, 484, 543
Hpy188III TCNNGA 2 cut(s) 135, 572
HpyAV CCTTC 5 cut(s) 365, 493, 528, 578, 627
HpyCH4IV ACGT 1 cut(s) 425
HpyCH4V TGCA 3 cut(s) 38, 63, 101
HpyF3I CTNAG 3 cut(s) 77, 104, 468
HpySE526I ACGT 1 cut(s) 425
HspAI GCGC 2 cut(s) 310, 612
Kpn2I TCCGGA 1 cut(s) 134
Kzo9I GATC 3 cut(s) 118, 452, 514
LmnI GCTCC 2 cut(s) 73, 485
Lsp1109I GCAGC 1 cut(s) 605
LweI GCATC 1 cut(s) 297
MaeI CTAG 2 cut(s) 411, 705
MaeII ACGT 1 cut(s) 425
MaeIII GTNAC 2 cut(s) 26, 655
MalI GATC 3 cut(s) 120, 454, 516
MboI GATC 3 cut(s) 118, 452, 514
MboII GAAGA 6 cut(s) 26, 167, 178, 350, 420, 669
MhlI GDGCHC 1 cut(s) 78
MluCI AATT 4 cut(s) 139, 170, 341, 475
MlyI GAGTC 1 cut(s) 62
MmeI TCCRAC 1 cut(s) 30
MnlI CCTC 4 cut(s) 142, 213, 417, 577
MroI TCCGGA 1 cut(s) 134
MroXI GAANNNNTTC 3 cut(s) 170, 567, 606
MslI CAYNNNNRTG 2 cut(s) 106, 272
MspI CCGG 2 cut(s) 116, 135
MspR9I CCNGG 2 cut(s) 116, 176
Mva1269I GAATGC 1 cut(s) 65
MvaI CCWGG 1 cut(s) 176
MvnI CGCG 2 cut(s) 212, 612
NciI CCSGG 1 cut(s) 116
NdeII GATC 3 cut(s) 118, 452, 514
NlaIV GGNNCC 2 cut(s) 113, 241
NmuCI GTSAC 1 cut(s) 26
PctI GAATGC 1 cut(s) 65
PdmI GAANNNNTTC 3 cut(s) 170, 567, 606
PfeI GAWTC 1 cut(s) 607
PfoI TCCNGGA 1 cut(s) 174
PkrI GCNGC 1 cut(s) 595
PleI GAGTC 1 cut(s) 62
PpsI GAGTC 1 cut(s) 62
Psp124BI GAGCTC 1 cut(s) 78
Psp6I CCWGG 1 cut(s) 174
PspGI CCWGG 1 cut(s) 174
PspN4I GGNNCC 2 cut(s) 113, 241
PspPI GGNCC 1 cut(s) 240
RsaI GTAC 2 cut(s) 230, 466
RsaNI GTAC 2 cut(s) 229, 465
RseI CAYNNNNRTG 2 cut(s) 106, 272
SacI GAGCTC 1 cut(s) 78
SatI GCNGC 1 cut(s) 594
Sau3AI GATC 3 cut(s) 118, 452, 514
Sau96I GGNCC 1 cut(s) 240
ScaI AGTACT 1 cut(s) 466
SchI GAGTC 1 cut(s) 62
ScrFI CCNGG 2 cut(s) 116, 176
SduI GDGCHC 1 cut(s) 78
SetI ASST 8 cut(s) 78, 224, 335, 428, 509, 548, 657, 706
SfaNI GCATC 1 cut(s) 297
SmiMI CAYNNNNRTG 2 cut(s) 106, 272
Sse9I AATT 4 cut(s) 139, 170, 341, 475
SsiI CCGC 2 cut(s) 5, 472
SspMI CTAG 2 cut(s) 411, 705
SstI GAGCTC 1 cut(s) 78
StyD4I CCNGG 2 cut(s) 114, 174
StyI CCWWGG 1 cut(s) 507
TaiI ACGT 1 cut(s) 428
TaqI TCGA 2 cut(s) 528, 631
TasI AATT 4 cut(s) 139, 170, 341, 475
TatI WGTACW 1 cut(s) 464
TfiI GAWTC 1 cut(s) 607
TscAI CASTG 4 cut(s) 31, 108, 256, 306
TseFI GTSAC 1 cut(s) 26
TseI GCWGC 1 cut(s) 593
Tsp45I GTSAC 1 cut(s) 26
TspDTI ATGAA 2 cut(s) 375, 503
TspRI CASTG 4 cut(s) 31, 108, 256, 306
XapI RAATTY 2 cut(s) 139, 170
XmnI GAANNNNTTC 3 cut(s) 170, 567, 606
XspI CTAG 2 cut(s) 411, 705
ZrmI AGTACT 1 cut(s) 466
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.