Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr9

Orthogroup ID

OG0003542

Physical Location & Seq

Genomic Coordinates Reverse (-)

8762195 .. 8767318

Gene Structure 5124 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3681 bp

ATGGCAGGAGGAAAGATAAGGGCGAAGCTTCGCCAGAGCAACCTTTACACATTCTGCCGTAAGCCTAAAGCCGATGAGACAGAGGCGCCTCGCCCAATCCAAGGAGCTGGGTTCACAAGAACTGTATATTGCAACCAACCTCATTTCCATCAAAAGAAGCCTTATAAATACCGTTCAAATTATATATCAACCACCAAGTATAACGTTATCACGTTCTTGCCCAAAGCACTCTTTGAGCAGTTCAGACGGGTTGCCAATGTGTATTTCCTTTTAGCTGCAATTCTTTCCCTCACACCTGTTTCACCTTTCTCTCCAGTGAGTATGATTGCTCCTCTGGTATTTGTTGTTGGTCTTAGTATGGCAAAGGAAGCGATGGAAGATTGGAATAGGTTCTTGCAGGATATGAAGGTTAATCGACGTAAAGTTATCGTTCATAAAGGAGATGGTGTTTTTGATTTTAGGCCATGGCATAAAATTCGGGTTGGAGATGTTGTGAAAGTGGAAAAGGATCGATTCTTTCCTGCAGATTTGCTTCTTTTGTCATCAAGTTATGAGGATGGGATTTGTTATGTTGAGACTATGAACTTAGATGGTGAAACAAATTTGAAGGTAAAGAGATGTTTGGAAGTAACCTCGATGGAGGATGATGGCGCTTTCAAGGATTTGACGGCAACAATTCAATGTGAAGATCCAAACCCCAATCTTTACTCATTCGTAGGTAATCTTGAGTACGATCGGCAGGTGTATCCTCTTGAGCCCAATCAGATACTCTTGAGAGATTCGAAGCTCAGGAATACAGCATATGTATATGGGGTGGTGATATTTACGGGTCATGATAGCAAAGTCATGCAGAATTCAACGAAGTCCCCTTCAAAAAGGAGCGGAATAGAAAGGAAAATGGATCACATTATCTATATTCTTTTCACCCTCCTTTTAGGGATTTCAATGATCAGCTCAATAGGCTTTGCTGTGAAGATAAAATTCAGCATGCCAGACTCGTGGTATTTACGACCTGATAATACAACGGATATGTATAGCCCAAAAAAGCCTGCCTTATCTGGCCTTATTCATTTGGTCACCGCTCTCATCCTTTATGGATATCTAATACCTATCTCTCTCTATGTTTCAATTGAGGTTGTGAAGGTTTTGCAAGCAAGCTTTATTAACCAAGACTTACTCATGTATGATGAAGAGACTGGGAAGCCTGCTGAAGCACGGACATCAAATTTGAATGAGGAGTTAGGCCAGGTAGACACGATCCTCTCTGATAAAACTGGCACATTAACCTGCAACCAGATGGACTTTCTGAAGTGTTCCATTGCTGGTACTGCATATGGCGTTCGTTCCAGTGAAGTTGAACTTGCAGCAGCAAAACAGATGGCTAGTGACCTTGAAGACCGTGATGATGAGCTTGCCAATTTTCCCATGCGAAAACATAATGCGCATGTATCATGGGAGAATGGAATAGGATCGGAAATTGAGCTAGAGACTGTTGTTACGTCCAATGAAGATAAGGATCGGAAACCTGCCATAAAGGGGTTTAGCTTTGAGGACAGCCGCCTCATGGGAGGAAATTGGTTGAATGAGTCCAGCCCTGATGTGATTTCATTATTTTTACGGATATTAGCAGTTTGCCACACTGCAATTCCAGAGGTAAATGAAGAGACTGGCAGTTATACATATGAAGCAGAGTCACCGGATGAAGCAGCTTTTCTTGTTGCAGCAAGAGAGTTAGGATTTGAGTTTTGTAAAAGAAATCAATCAGGTGTGTTTGTCCGCGAAAGGTATCATCATTCAGGACGACCAGTGGAAAGGGAGTACAAAATCTTAAATTTATTGGAATTCACTAGCAAAAGGAAGCGTATGTCTGTAATTGTGCGAGATGAAGATGGACAGCTTTTACTGATGTGCAAAGGAGCTGACAGCATAATTTTCGATCGACTTTCAAAGAATGGAAGAATGTATGAGGAAGCTACTACTAAGCATTTGAATGAATATGGAGAAGCTGGGTTGCGTACATTGGCACTTGCTTATAGAAAGCTTGAGGAGTCCGAGTACTCTGCTTGGAGTAATGAGTTTCAGAAAGCAAAAACATCTATTGGGGCTGATAGGGATGGAATGCTTGAGCGACTAGCAGATAAGATGGAAAGAGACTTGATCCTTGTTGGTGCTACAGCTGTAGAGGACAAATTGCAAAAAGGGGTGCCCCAGTGCATAGACAATCTCGCACAAGCTGGTCTCAAGATCTGGGTTTTGACAGGGGACAAGATGGAAACTGCAATCAACATAGGATTTGCATGTAGTTTGCTCCGACAGGGCATGAAGCAGATCTGTATATCAACCGCAAATTTAGACACGTTGGGACAAGATAGCAAAGAGGCGGTGAAAGATAACATTTTGAATCAAATCACCAATGCCTCACAAATGATCAAGTTGGAAAAAGATCCACATGCGGCATTTGCATTAATTATTGATGGAAAAACTTTAACCTATGCTTTGGAGGATGATATGAAGCATCTATTCTTGGGATTAGCTGTTGACTGTGCATCAGTCATATGCTGCCGTGTCTCCCCCAAGCAGAAGGCATTGGTAACAAGGTTAGTCAAACAAGGAACTGGAAAAACGACGTTAGCAATTGGGGATGGTGCAAATGATGTTGGAATGATTCAAGAAGCTGATATTGGTGTTGGAATCAGTGGGGTCGAAGGTATGCAGGCTGTGATGGCTAGTGACTTTGCTATTTCCCAGTTTCGGTATTTGGAGAGACTTTTGGTAGTCCACGGCCATTGGTGCTATAAGAGGATTGCTCAGATGGTCTGCTATTTCTTCTACAAAAATATTGCATTTGGTCTCACCCTCTTCTACTTCGAGGCATTCACGGGTTTCTCTGGGCAATCAATATATGATGACTGGTACATGTTATCATTCAATGTCATTCTAACCTCATTGCCTGTCATTTCACTTGGAGTTTTTGAACAAGATGTTTCGTCCGAGGTCTGCTTACAGTTCCCAGCATTGTACCAACAAGGACCTAGAAATTTGTTCTTCGATTGGTACAGGATACTGGGGTGGATGGGCAATGGTGTATACTGCTCTCTCATAATCTACTTCCTCAACATTATCATCTTCTACGACCAGGCCTTCCGTGCTAATGGCCAGACGGCTGATATGGCTGCTATGGGTACCACTATGTTCAGTTGCATCGTGTGGGCTGTGAACTGCCAGATTGCTCTCACAATGAGCCACTTTACGTGGATACAGCATCTCTTTGTGTGGGGAAGTATTGCAATGTGGTACCTATTTCTCTTAATTTACGGCATGCTGACTCCAACTTGGAGTAAGAATGCCTACCAAATTCTAGTCGAAGCTCTCGGTCCGGCCCCTTTGTTTTGGTCAGCCACCCTCTTAGTAACAATCGCTTGTAATCTCCCCTACATTGTCCACTTAGCCTTCCAAAGAAGTTTCAATCCAATGGACCATCATATCATCCAAGAAATCAAATACTACAAGAAGGATGTCGAGGATCAGCGCATGTGGAAGAGGGAGGCGTCGAAGGCAAGACAAGAAACCAAGATAGGGTTCACGGCGAGAGTGGATGCCAAGATCAGACACTTGAGAGGGAAGCTACAAAAGAAGCATCATACCCCTGTAAGTACGCAAGGAGGGATGTCACCATCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004012 GO:0005215 GO:0005319 GO:0005548 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006900 GO:0006996 GO:0008150 GO:0009987 GO:0010876 GO:0012505 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031984 GO:0033036 GO:0034204 GO:0042623 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045332 GO:0048193 GO:0048194 GO:0051179 GO:0051234 GO:0061024 GO:0065007 GO:0065008 GO:0071702 GO:0071840 GO:0071944 GO:0097035 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase E1-E2_ATPase PhoLip_ATPase_C PhoLip_ATPase_N

Protein Analysis

1227

Amino Acids

138.65

Weight (kDa)

6.81

Isoelectric Point (pI)

37.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PhoLip_ATPase_N	PF16209	42 - 109	6.4e-26	Phospholipid-translocating ATPase N-terminal
Cation_ATPase	PF13246	538 - 733	2.1e-22	P-type ATPase, cytoplasmic domain N
PhoLip_ATPase_C	PF16212	907 - 1157	5.4e-90	Phospholipid-translocating P-type ATPase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0003542)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G17500 AT1G17500 AT1G54280 AT1G54280 AT1G54280 AT1G72700 AT1G72700 AT1G72700 AT3G13900 AT3G13900 AT3G13900
fragaria_vesca	FvH4_6g34000 FvH4_6g34000 FvH4_6g34000 FvH4_6g34000 FvH4_6g34000 FvH4_6g34000 FvH4_6g34000 FvH4_6g34000 FvH4_6g34000
malus_domestica	MD09G1190200.v1.1 MD17G1173300.v1.1
prunus_persica	Prupe.3G046100_v2.0.a1 Prupe.3G046100_v2.0.a1
pyrus_communis	pycom09g10670 pycom17g17330
rosa_chinensis	RchiOBHm_Chr2g0143971
rosa_laevigata	RLG00000020069
rosa_multiflora	Rmu_ssc0000393.1_g000036
rosa_roxburghii	Rroxscaffold_2G00101430
rosa_rugosa	Rorug02G0385400 Rorug02G0385500
rosa_samantha	Rh2AG438000 Rh2BG446400
rosa_wichuraiana	Rw2G035770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	165
AarI	CACCTGC	1 cut(s)	730
Acc16I	TGCGCA	1 cut(s)	1443
Acc36I	ACCTGC	3 cut(s)	730, 1295, 1534
Acc65I	GGTACC	2 cut(s)	3182, 3294
AccB1I	GGYRCC	4 cut(s)	85, 2203, 3182, 3294
AccBSI	CCGCTC	2 cut(s)	882, 1082
AccI	GTMKAC	2 cut(s)	1251, 3087
AccII	CGCG	1 cut(s)	1779
AciI	CCGC	7 cut(s)	882, 1080, 1558, 1777, 2343, 2381, 2453
AclI	AACGTT	1 cut(s)	204
AclWI	GGATC	9 cut(s)	516, 683, 909, 1252, 1477, 1524, 2152, 2438, 3531
AcoI	YGGCCR	2 cut(s)	2782, 3154
AcuI	CTGAAG	2 cut(s)	1230, 1328
AcyI	GRCGYC	2 cut(s)	86, 3548
AfiI	CCNNNNNNNGG	5 cut(s)	101, 1536, 1564, 2751, 3576
AflIII	ACRYGT	2 cut(s)	2355, 2916
AjnI	CCWGG	2 cut(s)	1245, 3135
AjuI	GAANNNNNNNTTGG	6 cut(s)	248, 280, 2664, 2696, 3563, 3595
AleI	CACNNNNGTG	1 cut(s)	3676
AlwI	GGATC	9 cut(s)	516, 683, 909, 1252, 1477, 1524, 2152, 2438, 3531
AoxI	GGCC	7 cut(s)	461, 1060, 1243, 2782, 3138, 3154, 3378
ApeKI	GCWGC	7 cut(s)	275, 1364, 1367, 1706, 1721, 2559, 3173
ArsI	GACNNNNNNTTYG	2 cut(s)	1210, 1242
AseI	ATTAAT	1 cut(s)	2465
Asp700I	GAANNNNTTC	1 cut(s)	389
Asp718I	GGTACC	2 cut(s)	3182, 3294
AspLEI	GCGC	4 cut(s)	88, 653, 1444, 3531
AspS9I	GGNCC	4 cut(s)	3029, 3374, 3379, 3475
AsuII	TTCGAA	1 cut(s)	782
AvaII	GGWCC	3 cut(s)	3029, 3374, 3475
BaeGI	GKGCMC	1 cut(s)	2208
BaeI	ACNNNNGTAYC	2 cut(s)	3053, 3086
BalI	TGGCCA	1 cut(s)	3156
BanI	GGYRCC	4 cut(s)	85, 2203, 3182, 3294
BanII	GRGCYC	1 cut(s)	759
BauI	CACGAG	1 cut(s)	997
BbsI	GAAGAC	1 cut(s)	1401
BbvI	GCAGC	7 cut(s)	262, 1376, 1379, 1718, 1733, 2546, 3160
BceAI	ACGGC	7 cut(s)	42, 684, 2547, 2797, 3177, 3331, 3600
BcgI	CGANNNNNNTGC	6 cut(s)	458, 492, 1915, 1949, 2042, 2076
BciT130I	CCWGG	2 cut(s)	1247, 3137
BciVI	GTATCC	3 cut(s)	756, 3054, 3249
BclI	TGATCA	2 cut(s)	948, 2427
BfaI	CTAG	7 cut(s)	1383, 1484, 1848, 2132, 2727, 3033, 3359
BfmI	CTRYAG	3 cut(s)	522, 2172, 2178
BfoI	RGCGCY	2 cut(s)	89, 654
BfuAI	ACCTGC	3 cut(s)	730, 1295, 1534
BfuI	GTATCC	3 cut(s)	756, 3054, 3249
BglII	AGATCT	2 cut(s)	2244, 2328
BisI	GCNGC	9 cut(s)	276, 1365, 1368, 1558, 1707, 1722, 2454, 2560, 3174
BlsI	GCNGC	9 cut(s)	277, 1366, 1369, 1559, 1708, 1723, 2455, 2561, 3175
BmcAI	AGTACT	1 cut(s)	2057
Bme1390I	CCNGG	2 cut(s)	1247, 3137
Bme18I	GGWCC	3 cut(s)	3029, 3374, 3475
BmgT120I	GGNCC	4 cut(s)	3029, 3374, 3379, 3475
BmiI	GGNNCC	5 cut(s)	87, 2205, 3184, 3296, 3381
BmrFI	CCNGG	2 cut(s)	1247, 3137
BmrI	ACTGGG	4 cut(s)	1206, 2203, 2740, 3074
BmsI	GCATC	6 cut(s)	2524, 2555, 3210, 3271, 3586, 3646
BmuI	ACTGGG	4 cut(s)	1206, 2203, 2740, 3074
BpiI	GAAGAC	1 cut(s)	1401
BplI	GAGNNNNNCTC	2 cut(s)	2791, 2823
BpmI	CTGGAG	1 cut(s)	297
Bpu10I	CCTNAGC	1 cut(s)	788
Bpu14I	TTCGAA	1 cut(s)	782
BpuEI	CTTGAG	7 cut(s)	746, 773, 793, 2063, 2144, 2225, 3634
Bsa29I	ATCGAT	1 cut(s)	511
BsaAI	YACGTR	1 cut(s)	3252
BsaBI	GATNNNNATC	1 cut(s)	1515
BsaHI	GRCGYC	2 cut(s)	86, 3548
BsaI	GGTCTC	2 cut(s)	2243, 2855
BsaJI	CCNNGG	4 cut(s)	100, 464, 2779, 2991
BsaWI	WCCGGW	1 cut(s)	1696
BsaXI	ACNNNNNCTCC	6 cut(s)	432, 462, 477, 507, 2039, 2069
Bsc4I	CCNNNNNNNGG	5 cut(s)	101, 1536, 1564, 2751, 3576
Bse3DI	GCAATG	4 cut(s)	1317, 2945, 3085, 3294
Bse8I	GATNNNNATC	1 cut(s)	1515
BseBI	CCWGG	2 cut(s)	1247, 3137
BseCI	ATCGAT	1 cut(s)	511
BseDI	CCNNGG	4 cut(s)	100, 464, 2779, 2991
BseJI	GATNNNNATC	1 cut(s)	1515
BseLI	CCNNNNNNNGG	5 cut(s)	101, 1536, 1564, 2751, 3576
BseMI	GCAATG	4 cut(s)	1317, 2945, 3085, 3294
BseMII	CTCAG	2 cut(s)	802, 2822
BseRI	GAGGAG	3 cut(s)	321, 1250, 2060
BseSI	GKGCMC	1 cut(s)	2208
BseXI	GCAGC	7 cut(s)	262, 1376, 1379, 1718, 1733, 2546, 3160
BseYI	CCCAGC	3 cut(s)	107, 2006, 3010
Bsh1236I	CGCG	1 cut(s)	1779
Bsh1285I	CGRYCG	2 cut(s)	736, 1939
BshFI	GGCC	7 cut(s)	463, 1062, 1245, 2784, 3140, 3156, 3380
BshNI	GGYRCC	4 cut(s)	85, 2203, 3182, 3294
BshVI	ATCGAT	1 cut(s)	511
BsiEI	CGRYCG	2 cut(s)	736, 1939
BsiSI	CCGG	2 cut(s)	1697, 3377
BslFI	GGGAC	3 cut(s)	850, 2276, 2376
BslI	CCNNNNNNNGG	5 cut(s)	101, 1536, 1564, 2751, 3576
BsmFI	GGGAC	3 cut(s)	850, 2276, 2376
BsmI	GAATGC	3 cut(s)	2124, 2873, 3349
BsnI	GGCC	7 cut(s)	463, 1062, 1245, 2784, 3140, 3156, 3380
Bso31I	GGTCTC	2 cut(s)	2243, 2855
Bsp119I	TTCGAA	1 cut(s)	782
Bsp1286I	GDGCHC	2 cut(s)	759, 2208
Bsp19I	CCATGG	1 cut(s)	464
BspACI	CCGC	7 cut(s)	882, 1080, 1558, 1777, 2343, 2381, 2453
BspANI	GGCC	7 cut(s)	463, 1062, 1245, 2784, 3140, 3156, 3380
BspCNI	CTCAG	2 cut(s)	801, 2821
BspDI	ATCGAT	1 cut(s)	511
BspFNI	CGCG	1 cut(s)	1779
BspHI	TCATGA	1 cut(s)	832
BspLI	GGNNCC	5 cut(s)	87, 2205, 3184, 3296, 3381
BspMAI	CTGCAG	1 cut(s)	526
BspMI	ACCTGC	3 cut(s)	730, 1295, 1534
BspPI	GGATC	9 cut(s)	516, 683, 909, 1252, 1477, 1524, 2152, 2438, 3531
BspT104I	TTCGAA	1 cut(s)	782
BspT107I	GGYRCC	4 cut(s)	85, 2203, 3182, 3294
BspTNI	GGTCTC	2 cut(s)	2243, 2855
BsrBI	CCGCTC	2 cut(s)	882, 1082
BsrDI	GCAATG	4 cut(s)	1317, 2945, 3085, 3294
BssECI	CCNNGG	4 cut(s)	100, 464, 2779, 2991
BssNAI	GTATAC	1 cut(s)	3088
BssNI	GRCGYC	2 cut(s)	86, 3548
BssSI	CACGAG	1 cut(s)	997
BssT1I	CCWWGG	2 cut(s)	100, 464
Bst1107I	GTATAC	1 cut(s)	3088
Bst2BI	CACGAG	1 cut(s)	997
Bst2UI	CCWGG	2 cut(s)	1247, 3137
Bst4CI	ACNGT	6 cut(s)	124, 173, 1400, 1492, 2543, 3006
Bst6I	CTCTTC	4 cut(s)	1185, 1656, 2864, 3533
BstACI	GRCGYC	2 cut(s)	86, 3548
BstBAI	YACGTR	1 cut(s)	3252
BstBI	TTCGAA	1 cut(s)	782
BstC8I	GCNNGC	8 cut(s)	989, 1050, 1152, 1156, 1206, 1413, 2715, 3320
BstDEI	CTNAG	7 cut(s)	353, 586, 788, 1980, 2808, 3406, 3445
BstDSI	CCRYGG	2 cut(s)	464, 2779
BstEII	GGTNACC	1 cut(s)	1075
BstFNI	CGCG	1 cut(s)	1779
BstH2I	RGCGCY	2 cut(s)	89, 654
BstHHI	GCGC	4 cut(s)	88, 653, 1444, 3531
BstMCI	CGRYCG	2 cut(s)	736, 1939
BstMWI	GCNNNNNNNGC	9 cut(s)	368, 960, 1328, 2459, 2723, 2790, 3146, 3170, 3554
BstNI	CCWGG	2 cut(s)	1247, 3137
BstNSI	RCATGY	7 cut(s)	991, 1448, 2301, 2453, 2920, 3322, 3535
BstPI	GGTNACC	1 cut(s)	1075
BstSCI	CCNGG	2 cut(s)	1245, 3135
BstSFI	CTRYAG	3 cut(s)	522, 2172, 2178
BstSLI	GKGCMC	1 cut(s)	2208
BstUI	CGCG	1 cut(s)	1779
BstV1I	GCAGC	7 cut(s)	262, 1376, 1379, 1718, 1733, 2546, 3160
BstV2I	GAAGAC	1 cut(s)	1401
BstX2I	RGATCY	4 cut(s)	688, 2244, 2328, 2443
BstXI	CCANNNNNNTGG	2 cut(s)	107, 999
BstYI	RGATCY	4 cut(s)	688, 2244, 2328, 2443
BstZ17I	GTATAC	1 cut(s)	3088
Bsu15I	ATCGAT	1 cut(s)	511
BsuI	GTATCC	3 cut(s)	756, 3054, 3249
BsuRI	GGCC	7 cut(s)	463, 1062, 1245, 2784, 3140, 3156, 3380
BsuTUI	ATCGAT	1 cut(s)	511
BtgI	CCRYGG	2 cut(s)	464, 2779
BtgZI	GCGATG	1 cut(s)	386
BtsI	GCAGTG	1 cut(s)	1638
BtsIMutI	CAGTG	6 cut(s)	321, 1354, 1638, 1812, 2216, 2701
BveI	ACCTGC	3 cut(s)	730, 1295, 1534
Cac8I	GCNNGC	8 cut(s)	989, 1050, 1152, 1156, 1206, 1413, 2715, 3320
CciI	TCATGA	1 cut(s)	832
CfoI	GCGC	4 cut(s)	88, 653, 1444, 3531
Cfr13I	GGNCC	4 cut(s)	3029, 3374, 3379, 3475
ClaI	ATCGAT	1 cut(s)	511
CpoI	CGGWCCG	1 cut(s)	3374
CseI	GACGC	1 cut(s)	3537
CspCI	CAANNNNNGTGG	2 cut(s)	2768, 2803
CspI	CGGWCCG	1 cut(s)	3374
DdeI	CTNAG	7 cut(s)	353, 586, 788, 1980, 2808, 3406, 3445
DinI	GGCGCC	1 cut(s)	87
EaeI	YGGCCR	2 cut(s)	2782, 3154
Eam1104I	CTCTTC	4 cut(s)	1185, 1656, 2864, 3533
EarI	CTCTTC	4 cut(s)	1185, 1656, 2864, 3533
Eco130I	CCWWGG	2 cut(s)	100, 464
Eco147I	AGGCCT	1 cut(s)	3140
Eco24I	GRGCYC	1 cut(s)	759
Eco31I	GGTCTC	2 cut(s)	2243, 2855
Eco32I	GATATC	1 cut(s)	1100
Eco47I	GGWCC	3 cut(s)	3029, 3374, 3475
Eco57I	CTGAAG	2 cut(s)	1230, 1328
Eco91I	GGTNACC	1 cut(s)	1075
EcoO109I	RGGNCCY	1 cut(s)	3029
EcoO65I	GGTNACC	1 cut(s)	1075
EcoRI	GAATTC	2 cut(s)	853, 1841
EcoRII	CCWGG	2 cut(s)	1245, 3135
EcoRV	GATATC	1 cut(s)	1100
EcoT14I	CCWWGG	2 cut(s)	100, 464
EcoT38I	GRGCYC	1 cut(s)	759
EgeI	GGCGCC	1 cut(s)	87
EheI	GGCGCC	1 cut(s)	87
ErhI	CCWWGG	2 cut(s)	100, 464
FalI	AAGNNNNNCTT	4 cut(s)	1037, 1069, 1344, 1376
FaqI	GGGAC	3 cut(s)	850, 2276, 2376
FauNDI	CATATG	4 cut(s)	802, 1333, 1681, 2555
FbaI	TGATCA	2 cut(s)	948, 2427
FblI	GTMKAC	2 cut(s)	1251, 3087
Fnu4HI	GCNGC	9 cut(s)	276, 1365, 1368, 1558, 1707, 1722, 2454, 2560, 3174
FriOI	GRGCYC	1 cut(s)	759
Fsp4HI	GCNGC	9 cut(s)	276, 1365, 1368, 1558, 1707, 1722, 2454, 2560, 3174
FspAI	RTGCGCAY	1 cut(s)	1443
FspBI	CTAG	7 cut(s)	1383, 1484, 1848, 2132, 2727, 3033, 3359
FspI	TGCGCA	1 cut(s)	1443
GlaI	GCGC	4 cut(s)	87, 652, 1443, 3530
GluI	GCNGC	9 cut(s)	276, 1365, 1368, 1558, 1707, 1722, 2454, 2560, 3174
GsaI	CCCAGC	3 cut(s)	111, 2010, 3014
GsuI	CTGGAG	1 cut(s)	297
HaeII	RGCGCY	2 cut(s)	89, 654
HaeIII	GGCC	7 cut(s)	463, 1062, 1245, 2784, 3140, 3156, 3380
HapII	CCGG	2 cut(s)	1697, 3377
HgaI	GACGC	1 cut(s)	3537
HhaI	GCGC	4 cut(s)	88, 653, 1444, 3531
Hin1I	GRCGYC	2 cut(s)	86, 3548
Hin6I	GCGC	4 cut(s)	86, 651, 1442, 3529
HinP1I	GCGC	4 cut(s)	86, 651, 1442, 3529
HincII	GTYRAC	1 cut(s)	2539
HindII	GTYRAC	1 cut(s)	2539
HindIII	AAGCTT	3 cut(s)	26, 1156, 2039
HpaII	CCGG	2 cut(s)	1697, 3377
Hpy166II	GTNNAC	8 cut(s)	114, 1252, 2539, 2779, 3088, 3217, 3442, 3582
Hpy8I	GTNNAC	8 cut(s)	114, 1252, 2539, 2779, 3088, 3217, 3442, 3582
Hpy99I	CGWCG	3 cut(s)	420, 2629, 3553
HpyCH4III	ACNGT	6 cut(s)	124, 173, 1400, 1492, 2543, 3006
HpyCH4IV	ACGT	7 cut(s)	204, 212, 418, 1499, 2357, 2627, 3251
HpyF10VI	GCNNNNNNNGC	9 cut(s)	368, 960, 1328, 2459, 2723, 2790, 3146, 3170, 3554
HpyF3I	CTNAG	7 cut(s)	353, 586, 788, 1980, 2808, 3406, 3445
HpySE526I	ACGT	7 cut(s)	204, 212, 418, 1499, 2357, 2627, 3251
Hsp92I	GRCGYC	2 cut(s)	86, 3548
HspAI	GCGC	4 cut(s)	86, 651, 1442, 3529
KasI	GGCGCC	1 cut(s)	85
KpnI	GGTACC	2 cut(s)	3186, 3298
Ksp22I	TGATCA	2 cut(s)	948, 2427
LmnI	GCTCC	5 cut(s)	104, 334, 879, 1916, 2313
Lsp1109I	GCAGC	7 cut(s)	262, 1376, 1379, 1718, 1733, 2546, 3160
LweI	GCATC	6 cut(s)	2524, 2555, 3210, 3271, 3586, 3646
MaeI	CTAG	7 cut(s)	1383, 1484, 1848, 2132, 2727, 3033, 3359
MaeII	ACGT	7 cut(s)	204, 212, 418, 1499, 2357, 2627, 3251
MaeIII	GTNAC	9 cut(s)	628, 1075, 1385, 1495, 1692, 2590, 2729, 3409, 3669
MbiI	CCGCTC	2 cut(s)	882, 1082
MfeI	CAATTG	2 cut(s)	1128, 2634
MflI	RGATCY	4 cut(s)	688, 2244, 2328, 2443
MhlI	GDGCHC	2 cut(s)	759, 2208
MlsI	TGGCCA	1 cut(s)	3156
MluNI	TGGCCA	1 cut(s)	3156
Mly113I	GGCGCC	1 cut(s)	86
MlyI	GAGTC	5 cut(s)	989, 1595, 1700, 2057, 3319
MmeI	TCCRAC	6 cut(s)	463, 2335, 2415, 2638, 2668, 3353
Mox20I	TGGCCA	1 cut(s)	3156
MroXI	GAANNNNTTC	1 cut(s)	389
MscI	TGGCCA	1 cut(s)	3156
MseI	TTAA	7 cut(s)	411, 1164, 1283, 1829, 2465, 2486, 3308
MslI	CAYNNNNRTG	2 cut(s)	1989, 3676
Msp20I	TGGCCA	1 cut(s)	3156
MspA1I	CMGCKG	1 cut(s)	2177
MspI	CCGG	2 cut(s)	1697, 3377
MspR9I	CCNGG	2 cut(s)	1247, 3137
MunI	CAATTG	2 cut(s)	1128, 2634
Mva1269I	GAATGC	3 cut(s)	2124, 2873, 3349
MvaI	CCWGG	2 cut(s)	1247, 3137
MvnI	CGCG	1 cut(s)	1779
MwoI	GCNNNNNNNGC	9 cut(s)	368, 960, 1328, 2459, 2723, 2790, 3146, 3170, 3554
NarI	GGCGCC	1 cut(s)	86
NcoI	CCATGG	1 cut(s)	464
NdeI	CATATG	4 cut(s)	802, 1333, 1681, 2555
NlaIV	GGNNCC	5 cut(s)	87, 2205, 3184, 3296, 3381
NmuCI	GTSAC	5 cut(s)	1075, 1385, 1692, 2729, 3669
NsbI	TGCGCA	1 cut(s)	1443
NspI	RCATGY	7 cut(s)	991, 1448, 2301, 2453, 2920, 3322, 3535
NspV	TTCGAA	1 cut(s)	782
OliI	CACNNNNGTG	1 cut(s)	3676
PaeI	GCATGC	2 cut(s)	991, 3322
PagI	TCATGA	1 cut(s)	832
PaqCI	CACCTGC	1 cut(s)	730
PceI	AGGCCT	1 cut(s)	3140
PciI	ACATGT	1 cut(s)	2916
PctI	GAATGC	3 cut(s)	2124, 2873, 3349
PdmI	GAANNNNTTC	1 cut(s)	389
PfeI	GAWTC	5 cut(s)	513, 779, 2401, 2665, 2691
PkrI	GCNGC	9 cut(s)	277, 1366, 1369, 1559, 1708, 1723, 2455, 2561, 3175
Ple19I	CGATCG	2 cut(s)	736, 1939
PleI	GAGTC	5 cut(s)	989, 1594, 1699, 2056, 3319
PluTI	GGCGCC	1 cut(s)	89
PpsI	GAGTC	5 cut(s)	989, 1594, 1699, 2056, 3319
Ppu21I	YACGTR	1 cut(s)	3252
PpuMI	RGGWCCY	1 cut(s)	3029
PscI	ACATGT	1 cut(s)	2916
PshBI	ATTAAT	1 cut(s)	2465
PsiI	TTATAA	1 cut(s)	165
Psp1406I	AACGTT	1 cut(s)	204
Psp5II	RGGWCCY	1 cut(s)	3029
Psp6I	CCWGG	2 cut(s)	1245, 3135
PspEI	GGTNACC	1 cut(s)	1075
PspFI	CCCAGC	3 cut(s)	107, 2006, 3010
PspGI	CCWGG	2 cut(s)	1245, 3135
PspN4I	GGNNCC	5 cut(s)	87, 2205, 3184, 3296, 3381
PspPI	GGNCC	4 cut(s)	3029, 3374, 3379, 3475
PspPPI	RGGWCCY	1 cut(s)	3029
PstI	CTGCAG	1 cut(s)	526
PsuI	RGATCY	4 cut(s)	688, 2244, 2328, 2443
PvuI	CGATCG	2 cut(s)	736, 1939
PvuII	CAGCTG	1 cut(s)	2177
RseI	CAYNNNNRTG	2 cut(s)	1989, 3676
Rsr2I	CGGWCCG	1 cut(s)	3374
RsrII	CGGWCCG	1 cut(s)	3374
SaqAI	TTAA	7 cut(s)	411, 1164, 1283, 1829, 2465, 2486, 3308
SatI	GCNGC	9 cut(s)	276, 1365, 1368, 1558, 1707, 1722, 2454, 2560, 3174
Sau96I	GGNCC	4 cut(s)	3029, 3374, 3379, 3475
ScaI	AGTACT	1 cut(s)	2057
SchI	GAGTC	5 cut(s)	989, 1595, 1700, 2057, 3319
ScrFI	CCNGG	2 cut(s)	1247, 3137
SduI	GDGCHC	2 cut(s)	759, 2208
SfaNI	GCATC	6 cut(s)	2524, 2555, 3210, 3271, 3586, 3646
SfcI	CTRYAG	3 cut(s)	522, 2172, 2178
SfoI	GGCGCC	1 cut(s)	87
SfuI	TTCGAA	1 cut(s)	782
SinI	GGWCC	3 cut(s)	3029, 3374, 3475
SmiMI	CAYNNNNRTG	2 cut(s)	1989, 3676
SmlI	CTYRAG	7 cut(s)	725, 752, 772, 2042, 2123, 2240, 3613
SmoI	CTYRAG	7 cut(s)	725, 752, 772, 2042, 2123, 2240, 3613
SphI	GCATGC	2 cut(s)	991, 3322
SseBI	AGGCCT	1 cut(s)	3140
SsiI	CCGC	7 cut(s)	882, 1080, 1558, 1777, 2343, 2381, 2453
SspDI	GGCGCC	1 cut(s)	85
SspI	AATATT	1 cut(s)	2839
SspMI	CTAG	7 cut(s)	1383, 1484, 1848, 2132, 2727, 3033, 3359
StuI	AGGCCT	1 cut(s)	3140
StyD4I	CCNGG	2 cut(s)	1245, 3135
StyI	CCWWGG	2 cut(s)	100, 464
TaaI	ACNGT	6 cut(s)	124, 173, 1400, 1492, 2543, 3006
TaiI	ACGT	7 cut(s)	207, 215, 421, 1502, 2360, 2630, 3254
TaqII	GACCGA	1 cut(s)	3362
TatI	WGTACW	2 cut(s)	1818, 2055
TauI	GCSGC	2 cut(s)	1560, 2456
TfiI	GAWTC	5 cut(s)	513, 779, 2401, 2665, 2691
Tru1I	TTAA	7 cut(s)	411, 1164, 1283, 1829, 2465, 2486, 3308
Tru9I	TTAA	7 cut(s)	411, 1164, 1283, 1829, 2465, 2486, 3308
TscAI	CASTG	6 cut(s)	321, 1354, 1645, 1812, 2216, 2701
TseFI	GTSAC	5 cut(s)	1075, 1385, 1692, 2729, 3669
TseI	GCWGC	7 cut(s)	275, 1364, 1367, 1706, 1721, 2559, 3173
Tsp45I	GTSAC	5 cut(s)	1075, 1385, 1692, 2729, 3669
TspGWI	ACGGA	4 cut(s)	1040, 1231, 1633, 3134
TspRI	CASTG	6 cut(s)	321, 1354, 1645, 1812, 2216, 2701
VpaK11BI	GGWCC	3 cut(s)	3029, 3374, 3475
VspI	ATTAAT	1 cut(s)	2465
XceI	RCATGY	7 cut(s)	991, 1448, 2301, 2453, 2920, 3322, 3535
XmiI	GTMKAC	2 cut(s)	1251, 3087
XmnI	GAANNNNTTC	1 cut(s)	389
XspI	CTAG	7 cut(s)	1383, 1484, 1848, 2132, 2727, 3033, 3359
ZrmI	AGTACT	1 cut(s)	2057

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.