pycom09g11060

Ribonuclease III domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0003592
Physical Location & Seq
Reverse (-)
9214762 .. 9218339
3578 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 768 bp
ATGCAAACTCTATCAGTGGCTTTTCCGCCGATGAGTGCGAGACTAGTGACAGTGAGAGCTTCCGTGGATACACAGCAAAGACTCTCATACAACCGCAACGCTCCAAGAAAGATACAGAAAAGCGAAAGCCCCGCCACCGCCAAACCTCCTCCAACCGTCACACTAACCACCACAACTACCCGAACGGCCGACTCCGTTGTTGACCTCCTCAAACGCACACCTCAAGGGGAAACAGTACAAATGGATGAGTTATACTTGGGCTATGAGCGATGGTCACCTACTCCAACAAAGGTACAAAAGCGTTTGCCTAGTCCACCAAAGGTACAGAAGCCTCGGTCTGTACTCAATGCGGCATCGCTAGCTTATATCGGTGATTGCATGTACGAGCTATATGCTCGCAGGCATTTTTTGTTTCCTCCCCTGAATATTGAAGAATATAATGATCGTGTGATGGCAGTTGTACGTTGTGAAGCACAGGATGCACTGCTCCGGAAACTTCTGAGTGATGATTTTTTATTAGAAGAAGAAAGGAATGTTCTCCGGTGGGGAAAGAATATTGGTTCAGCAAAAACCCGAACAAAAAAACGTGCTGGTTCAGCAGCTTATAACAGAGCATCTTCCTTGGAAGCATTGGTTGGTTATCTCTACCTGACAAATATGGATCGCTTAGAAGAAGTCATGATAAAGTTGGGATTCTCAACTGATTCTTCTGTGCAGATAATTTCAGAGGAGCCTGAGGAGGTAAACGGCACGCATCCTAGTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_3

Protein Analysis

256

Amino Acids

28.74

Weight (kDa)

9.13

Isoelectric Point (pI)

54.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_3 PF00636 119 - 219 1.9e-21 Ribonuclease III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 606
AccIII TCCGGA 1 cut(s) 489
AciI CCGC 5 cut(s) 26, 94, 132, 138, 350
AclWI GGATC 1 cut(s) 669
AcoI YGGCCR 1 cut(s) 186
AfaI GTAC 6 cut(s) 237, 294, 324, 342, 383, 462
AgsI TTSAA 1 cut(s) 431
AhlI ACTAGT 1 cut(s) 43
AjuI GAANNNNNNNTTGG 2 cut(s) 618, 650
AluBI AGCT 4 cut(s) 59, 362, 388, 602
AluI AGCT 4 cut(s) 59, 362, 388, 602
Alw26I GTCTC 1 cut(s) 34
AlwI GGATC 1 cut(s) 669
Aor13HI TCCGGA 1 cut(s) 489
AoxI GGCC 1 cut(s) 186
ApeKI GCWGC 1 cut(s) 599
AsuHPI GGTGA 2 cut(s) 267, 383
AsuNHI GCTAGC 1 cut(s) 358
AxyI CCTNAGG 1 cut(s) 735
BbvI GCAGC 1 cut(s) 611
BccI CCATC 2 cut(s) 264, 445
BceAI ACGGC 2 cut(s) 201, 763
BcgI CGANNNNNNTGC 2 cut(s) 374, 408
BciVI GTATCC 1 cut(s) 61
BcoDI GTCTC 1 cut(s) 34
BcuI ACTAGT 1 cut(s) 43
BfaI CTAG 4 cut(s) 44, 309, 359, 759
BfuI GTATCC 1 cut(s) 61
BisI GCNGC 2 cut(s) 351, 600
BlsI GCNGC 2 cut(s) 352, 601
BmiI GGNNCC 1 cut(s) 732
BmsI GCATC 4 cut(s) 362, 469, 623, 763
BmtI GCTAGC 1 cut(s) 362
BpuEI CTTGAG 1 cut(s) 207
BsaJI CCNNGG 3 cut(s) 63, 332, 621
BsaWI WCCGGW 2 cut(s) 489, 540
Bse21I CCTNAGG 1 cut(s) 735
BseAI TCCGGA 1 cut(s) 489
BseDI CCNNGG 3 cut(s) 63, 332, 621
BseGI GGATG 3 cut(s) 250, 484, 754
BseMII CTCAG 2 cut(s) 491, 726
BseRI GAGGAG 4 cut(s) 138, 197, 743, 752
BseX3I CGGCCG 1 cut(s) 186
BseXI GCAGC 1 cut(s) 611
BsgI GTGCAG 1 cut(s) 734
Bsh1285I CGRYCG 1 cut(s) 189
BshFI GGCC 1 cut(s) 188
BsiEI CGRYCG 1 cut(s) 189
BsiSI CCGG 2 cut(s) 490, 541
BsmAI GTCTC 1 cut(s) 34
BsnI GGCC 1 cut(s) 188
Bsp13I TCCGGA 1 cut(s) 489
Bsp143I GATC 2 cut(s) 442, 661
BspACI CCGC 5 cut(s) 26, 94, 132, 138, 350
BspANI GGCC 1 cut(s) 188
BspCNI CTCAG 2 cut(s) 492, 727
BspEI TCCGGA 1 cut(s) 489
BspHI TCATGA 1 cut(s) 678
BspLI GGNNCC 1 cut(s) 732
BspOI GCTAGC 1 cut(s) 362
BspPI GGATC 1 cut(s) 669
BssECI CCNNGG 3 cut(s) 63, 332, 621
BssMI GATC 2 cut(s) 442, 661
BssT1I CCWWGG 1 cut(s) 621
Bst4CI ACNGT 3 cut(s) 52, 157, 235
BstAPI GCANNNNNTGC 1 cut(s) 479
BstC8I GCNNGC 4 cut(s) 360, 397, 401, 752
BstDEI CTNAG 3 cut(s) 500, 667, 735
BstDSI CCRYGG 1 cut(s) 63
BstEII GGTNACC 1 cut(s) 273
BstF5I GGATG 3 cut(s) 250, 484, 754
BstKTI GATC 2 cut(s) 445, 664
BstMAI GTCTC 1 cut(s) 34
BstMBI GATC 2 cut(s) 442, 661
BstMCI CGRYCG 1 cut(s) 189
BstMWI GCNNNNNNNGC 3 cut(s) 359, 479, 596
BstNSI RCATGY 1 cut(s) 382
BstPI GGTNACC 1 cut(s) 273
BstV1I GCAGC 1 cut(s) 611
BstZI CGGCCG 1 cut(s) 186
Bsu36I CCTNAGG 1 cut(s) 735
BsuI GTATCC 1 cut(s) 61
BsuRI GGCC 1 cut(s) 188
BtgI CCRYGG 1 cut(s) 63
BtgZI GCGATG 2 cut(s) 283, 339
BtsCI GGATG 3 cut(s) 250, 484, 754
BtsI GCAGTG 1 cut(s) 482
BtsIMutI CAGTG 3 cut(s) 21, 57, 482
Cac8I GCNNGC 4 cut(s) 360, 397, 401, 752
CciI TCATGA 1 cut(s) 678
Csp6I GTAC 6 cut(s) 236, 293, 323, 341, 382, 461
CviAII CATG 2 cut(s) 379, 679
CviQI GTAC 6 cut(s) 236, 293, 323, 341, 382, 461
DdeI CTNAG 3 cut(s) 500, 667, 735
DpnI GATC 2 cut(s) 444, 663
DpnII GATC 2 cut(s) 442, 661
EaeI YGGCCR 1 cut(s) 186
EagI CGGCCG 1 cut(s) 186
EciI GGCGGA 1 cut(s) 15
EclXI CGGCCG 1 cut(s) 186
Eco130I CCWWGG 1 cut(s) 621
Eco52I CGGCCG 1 cut(s) 186
Eco81I CCTNAGG 1 cut(s) 735
Eco91I GGTNACC 1 cut(s) 273
EcoO65I GGTNACC 1 cut(s) 273
EcoT14I CCWWGG 1 cut(s) 621
ErhI CCWWGG 1 cut(s) 621
FaeI CATG 2 cut(s) 382, 682
FatI CATG 2 cut(s) 378, 678
FauI CCCGC 1 cut(s) 139
Fnu4HI GCNGC 2 cut(s) 351, 600
FokI GGATG 3 cut(s) 257, 491, 741
Fsp4HI GCNGC 2 cut(s) 351, 600
FspBI CTAG 4 cut(s) 44, 309, 359, 759
GluI GCNGC 2 cut(s) 351, 600
HaeIII GGCC 1 cut(s) 188
HapII CCGG 2 cut(s) 490, 541
Hin1II CATG 2 cut(s) 382, 682
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HinfI GANTC 4 cut(s) 81, 191, 693, 704
HpaII CCGG 2 cut(s) 490, 541
HphI GGTGA 2 cut(s) 267, 383
Hpy166II GTNNAC 3 cut(s) 202, 314, 745
Hpy188I TCNGA 2 cut(s) 501, 727
Hpy188III TCNNGA 2 cut(s) 490, 679
Hpy8I GTNNAC 3 cut(s) 202, 314, 745
HpyCH4III ACNGT 3 cut(s) 52, 157, 235
HpyCH4IV ACGT 2 cut(s) 463, 586
HpyCH4V TGCA 4 cut(s) 4, 378, 482, 715
HpyF10VI GCNNNNNNNGC 3 cut(s) 359, 479, 596
HpyF3I CTNAG 3 cut(s) 500, 667, 735
HpySE526I ACGT 2 cut(s) 463, 586
Hsp92II CATG 2 cut(s) 382, 682
Kpn2I TCCGGA 1 cut(s) 489
Kzo9I GATC 2 cut(s) 442, 661
LmnI GCTCC 3 cut(s) 106, 492, 730
LpnPI CCDG 8 cut(s) 385, 434, 461, 503, 554, 576, 662, 747
Lsp1109I GCAGC 1 cut(s) 611
LweI GCATC 4 cut(s) 362, 469, 623, 763
MaeI CTAG 4 cut(s) 44, 309, 359, 759
MaeII ACGT 2 cut(s) 463, 586
MaeIII GTNAC 3 cut(s) 46, 157, 273
MalI GATC 2 cut(s) 444, 663
MboI GATC 2 cut(s) 442, 661
MboII GAAGA 6 cut(s) 443, 533, 536, 609, 683, 699
MluCI AATT 1 cut(s) 720
MlyI GAGTC 2 cut(s) 75, 185
MmeI TCCRAC 2 cut(s) 176, 308
MroI TCCGGA 1 cut(s) 489
MslI CAYNNNNRTG 1 cut(s) 759
MspI CCGG 2 cut(s) 490, 541
MwoI GCNNNNNNNGC 3 cut(s) 359, 479, 596
NdeII GATC 2 cut(s) 442, 661
NheI GCTAGC 1 cut(s) 358
NlaIII CATG 2 cut(s) 382, 682
NlaIV GGNNCC 1 cut(s) 732
NmuCI GTSAC 3 cut(s) 46, 157, 273
NspI RCATGY 1 cut(s) 382
PagI TCATGA 1 cut(s) 678
PfeI GAWTC 2 cut(s) 693, 704
PkrI GCNGC 2 cut(s) 352, 601
PleI GAGTC 2 cut(s) 75, 185
PpsI GAGTC 2 cut(s) 75, 185
PsiI TTATAA 1 cut(s) 606
PspEI GGTNACC 1 cut(s) 273
PspN4I GGNNCC 1 cut(s) 732
RsaI GTAC 6 cut(s) 237, 294, 324, 342, 383, 462
RsaNI GTAC 6 cut(s) 236, 293, 323, 341, 382, 461
RseI CAYNNNNRTG 1 cut(s) 759
SatI GCNGC 2 cut(s) 351, 600
Sau3AI GATC 2 cut(s) 442, 661
SchI GAGTC 2 cut(s) 75, 185
SfaNI GCATC 4 cut(s) 362, 469, 623, 763
SmiMI CAYNNNNRTG 1 cut(s) 759
SmlI CTYRAG 1 cut(s) 222
SmoI CTYRAG 1 cut(s) 222
SpeI ACTAGT 1 cut(s) 43
Sse9I AATT 1 cut(s) 720
SsiI CCGC 5 cut(s) 26, 94, 132, 138, 350
SspI AATATT 2 cut(s) 427, 556
SspMI CTAG 4 cut(s) 44, 309, 359, 759
StyI CCWWGG 1 cut(s) 621
TaaI ACNGT 3 cut(s) 52, 157, 235
TaiI ACGT 2 cut(s) 466, 589
TaqII GACCGA 1 cut(s) 324
TasI AATT 1 cut(s) 720
TatI WGTACW 2 cut(s) 235, 340
TauI GCSGC 1 cut(s) 353
TfiI GAWTC 2 cut(s) 693, 704
TscAI CASTG 3 cut(s) 21, 57, 489
TseFI GTSAC 3 cut(s) 46, 157, 273
TseI GCWGC 1 cut(s) 599
Tsp45I GTSAC 3 cut(s) 46, 157, 273
TspGWI ACGGA 2 cut(s) 52, 184
TspRI CASTG 3 cut(s) 21, 57, 489
XceI RCATGY 1 cut(s) 382
XspI CTAG 4 cut(s) 44, 309, 359, 759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.