Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr9

Orthogroup ID

OG0005600

Physical Location & Seq

Genomic Coordinates Forward (+)

15712657 .. 15718139

Gene Structure 5483 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2901 bp

ATGGCTGCAAGGAACTTGGAGAAGATGGCTTCAATTGACGCCCAACTGAGGCTTTTGGCACCTGGGAAGGTCTCTGAGGATGACAAACTGGTTGAGTACGATGCTCTGCTATTGGATCGCTTCCTTGATATTCTTCAGGATTTACATGGAGAGAGTCTCAGAGAAACGGTGCAAGATTGTTATGAGCTTTCCGCGGAATATGAAAGAAAGCAGGACCCTCAAAAGTTGGAGGAACTTGGCAAAATGCTAACGAGTTTGGATCCCGGGGACTCCATTGTCATTGCAAAATCCTTTTCTCACATGCTTAACTTGGCAAATTTGGCCGAGGAAGTTCAGATTGCGTACCGGAGGAGGATCAAGTTGAAGAAGGGAGATTTTGCTGATGAGGCGTCGGCTACTACGGAGTCAGACATTGAAGAGACTCTCAAGAGGCTTGTGGGGGACCTGAAAAAGTCCCCAGAAGAAGTTTTTGATGCCTTGAAGAACCAGACTGTAGATTTGGTTCTGACTGCACATCCTACTCAGTCTGTTCGCAGGTCTTTGCTTCAAAAGCATGCAAGGATAAGAAATTGTTTGACGCAGTTGTATGCTAAGGACATTACGCCTGATGATAAGCAGGAACTCGATGAGGCTTTACAAAGGGAGATTCAAGCTGCATTTCGTACAGATGAAATCCGAAGGACTCCTCCAACCCCTCAAGATGAGATGAGGGCAGGAATGAGCTACTTCCATGAGACAATTTGGAAAGGTGTACCAAAATTCTTACGTCGTGTTGACACTGCTTTGAAGAACATAGGGATAAACGAACGTGTTCCTTACAATGCCCCTCTCATTCAGTTTTCTTCTTGGATGGGTGGGGATCGTGATGGAAACCCCAGGGTCACTCCGGAAGTTACTCGGGATGTATGCTTATTGGCTAGAATGATGGCTGCTAACTTATACTTCTCCCAGATAGAGGATCTTATGTTTGAGTTATCTATGTGGCGATGCAATGATGAGCTTCGCACTCGTGCTGATGAACTTCATAGGTCTTCAAAGAAGGATGCAAAACAGTACATAGAGTTTTGGAAAAAAATTCCTCCAAATGAGCCTTATCGTGTTATTCTTGGTGATGTAAGAGACAAGCTTTACAATACGCGTGAACGTGCTCGCCAATTATTAGCCAATGGAGTCTCTGACATTCATGAGGACACAACGTTTACCAATGTTGAACAGTTTCTGGAGCCTCTGGAACTTTGTTACCGGTCACTCTGCTCCTGTGGCGATCGAGTAATTGCTGATGGAAGCCTTCTTGATTTCTTACGACAAGTTTCTACTTTTGGGCTGTCTCTTGTGAGACTAGACATACGACAAGAATCAGACAGGCACACTGATGTCATCAATGCTATAACAAAACACCTGGAGATAGGGTCTTATCGAGAATGGTCTGAGGAGCGTCGGCAAGAATGGCTTTTGTCTGAGCTTCGTGGCAAGCGCCCCCTTTTTGGCCGTGATGTTCCCAAGACTGAAGAAATTGCTGATGTGCTGGAAACGTTCCATGTCATTTCAGAACTTCCCTCAGACAATTTTGGTGCTTATATTATATCAATGGCAACAGCCCCATCTGATGTTCTCGCTGTTGAGCTTTTACAACGTGAATGTGGTGTGAAGAAGCCACTGAGGGTTGTTCCATTGTTTGAGAAGCTTGCTGATCTTGAGGCTGCTCCTGCTGCTGTGGCTCGTCTCTTTTCAATAGACTGGTACAAAGATCGGATCAATGGGAAGCAAGAAGTCATGATAGGGTACTCAGATTCCGGGAAGGATGCTGGTCGTTTATCTGCAGCATGGCAGTTATACAAGGCTCAGGAAGAGCTCATAAAGGTTGCAAAACAATTTGGGGTTAAGCTTACGATGTTTCATGGTCGAGGTGGAACAGTTGGAAGAGGAGGGGGGCCCACCCATCTTGCTATATTGTCTCAGCCACCCGACACTATTCATGGTTCGCTTCGTGTTACAGTTCAAGGTGAAGTTATTGAACAATCATTTGGGGAGGAACACTTGTGCTTTAGAACACTCCAGCGTTTCACAGCAGCTACACTTGAGCATGGAATGCATCCTCCAGTCTCCCCAAAGCCAGAATGGCGTGCACTCATGGATGAAATGGCCGTCATTGCAACAAAGGAATATCGTTCAATAGTTTTCCAGGAGCCCCGTTTTGTGGAATACTTTCGCCTCGCAACACCGGAGTTGGAGTATGGTCGAATGAACATTGGGAGTCGTCCTTCAAAACGAAAGCCCAGTGGAGGCATTGAATCACTTCGTGCAATCCCATCGATCTTTGCTTGGACCCAGACAAGATTTCACTTACCAGTGTGGCTTGGCTTCGGGGCAGCATTTAAGCATGCTGTTGAGAAGGATGCAAAGAATCTTCAAATGCTCCAGGAGATGTATAACCAGTGGCCTTTTTTCAGAGTCACAATTGATTTAATTGAGATGGTGTTTGCCAAGGTCGATCCGGGTATTGCCTCTCTCTATGACAAGCTTCTTGTGTCAAAAGACCTGTGGTCATTTGGAGACCGATTGAGGTCCAACTATGAAGAAACTAAGCGCCTCGTCCTCCAGGTTGCTGGACATAGGGCTCTTCTGGAAGGAGATCCTTATTTGAGGCAGCGACTCCTCCTTCGTGATTCATATATCACAACACTTAACGTGTGCCAAGCCTATACACTGAAGCAGATTCGTGATCCAAACTACCATGTTAAAGTAAGGCCACACTTGTCAAAGGAGTACATGGAAACAAGAAGCAAGCCGGCAGCGGAGCTTGTGAAACTCAATCCAACGAGCGAGTATGCTCCTGGTCTAGAGGACACGCTTATTCTGACCATGAAGGGTATTGCTGCTGGCATGCAGAACACCGGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004611 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0007275 GO:0008150 GO:0008152 GO:0008964 GO:0009506 GO:0009507 GO:0009536 GO:0015977 GO:0016020 GO:0016829 GO:0016830 GO:0016831 GO:0030054 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0048046 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0055044 GO:0071704 GO:0071944 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko00620 ko00680 ko00710 ko00720 ko01100 ko01120 ko01200 map00620 map00680 map00710 map00720 map01100 map01120 map01200

Pfam Domains

Protein Families

PEPcase

Protein Analysis

967

Amino Acids

110.39

Weight (kDa)

6.24

Isoelectric Point (pI)

45.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PEPcase	PF00311	34 - 966	0.0	Phosphoenolpyruvate carboxylase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0005600)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G42600 AT2G42600 AT2G42600
fragaria_vesca	FvH4_6g27390 FvH4_6g27390 FvH4_6g27390 FvH4_6g33320
malus_domestica	MD09G1237900.v1.1 MD17G1230800.v1.1
prunus_persica	Prupe.3G118300_v2.0.a1
pyrus_communis	pycom09g15640 pycom17g23400
rosa_chinensis	RchiOBHm_Chr2g0090101 RchiOBHm_Chr2g0129661
rosa_laevigata	RLG00000016085 RLG00000019094
rosa_multiflora	Rmu_sc0008968.1_g000006 Rmu_sc0009263.1_g000005 Rmu_sc0020548.1_g000003
rosa_roxburghii	Rroxscaffold_2G00114470
rosa_rugosa	Rorug02G0005300 Rorug02G0288200
rosa_samantha	Rh2AG339500 Rh2BG348300 Rh2CG326900 Rh2DG366400
rosa_wichuraiana	Rw2G004560 Rw2G027850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	275
Acc36I	ACCTGC	1 cut(s)	525
AccB1I	GGYRCC	1 cut(s)	58
AccII	CGCG	2 cut(s)	194, 1138
AccIII	TCCGGA	1 cut(s)	886
AciI	CCGC	3 cut(s)	192, 194, 2795
AclI	AACGTT	2 cut(s)	1196, 1532
AcoI	YGGCCR	3 cut(s)	321, 1486, 2142
AcsI	RAATTY	3 cut(s)	316, 758, 1074
AcuI	CTGAAG	3 cut(s)	119, 1527, 2729
AcyI	GRCGYC	2 cut(s)	39, 389
AdeI	CACNNNGTG	1 cut(s)	2300
AfaI	GTAC	8 cut(s)	98, 344, 664, 753, 1055, 1742, 1784, 2768
AfiI	CCNNNNNNNGG	5 cut(s)	48, 955, 1484, 2197, 2282
AflIII	ACRYGT	3 cut(s)	808, 1136, 2688
AgeI	ACCGGT	2 cut(s)	1242, 2894
AhdI	GACNNNNNGTC	1 cut(s)	2542
AjnI	CCWGG	7 cut(s)	61, 875, 1398, 2181, 2418, 2598, 2833
AjuI	GAANNNNNNNTTGG	2 cut(s)	2007, 2039
Alw21I	GWGCWC	3 cut(s)	1150, 1854, 2128
Alw44I	GTGCAC	1 cut(s)	2124
AlwNI	CAGNNNCTG	1 cut(s)	1219
Ama87I	CYCGRG	2 cut(s)	263, 897
Aor13HI	TCCGGA	1 cut(s)	886
AoxI	GGCC	6 cut(s)	321, 1486, 1931, 2142, 2438, 2747
ApaI	GGGCCC	1 cut(s)	1935
ApaLI	GTGCAC	1 cut(s)	2124
ApoI	RAATTY	3 cut(s)	316, 758, 1074
ArsI	GACNNNNNNTTYG	4 cut(s)	481, 513, 2643, 2675
AsiGI	ACCGGT	2 cut(s)	1242, 2894
Asp700I	GAANNNNTTC	4 cut(s)	810, 1215, 2205, 2295
AspLEI	GCGC	2 cut(s)	1476, 2589
AspS9I	GGNCC	6 cut(s)	214, 442, 1931, 1932, 2325, 2565
AsuC2I	CCSGG	4 cut(s)	264, 265, 1795, 2496
AsuHPI	GGTGA	2 cut(s)	1121, 2015
AvaI	CYCGRG	2 cut(s)	263, 897
AvaII	GGWCC	4 cut(s)	214, 442, 2325, 2565
BaeGI	GKGCMC	2 cut(s)	1935, 2128
BamHI	GGATCC	1 cut(s)	259
BanI	GGYRCC	1 cut(s)	58
BanII	GRGCYC	4 cut(s)	1854, 1935, 2190, 2620
BauI	CACGAG	1 cut(s)	1008
BbsI	GAAGAC	1 cut(s)	1023
Bbv12I	GWGCWC	3 cut(s)	1150, 1854, 2128
BccI	CCATC	9 cut(s)	19, 844, 860, 919, 1274, 1609, 1947, 2317, 2467
BceAI	ACGGC	2 cut(s)	1473, 2129
BcgI	CGANNNNNNTGC	2 cut(s)	2292, 2326
BciT130I	CCWGG	7 cut(s)	63, 877, 1400, 2183, 2420, 2600, 2835
BcnI	CCSGG	4 cut(s)	264, 265, 1795, 2496
BfaI	CTAG	3 cut(s)	918, 1340, 2840
BfmI	CTRYAG	2 cut(s)	492, 1818
BfoI	RGCGCY	2 cut(s)	1477, 2590
BfuAI	ACCTGC	1 cut(s)	525
BglI	GCCNNNNNGGC	1 cut(s)	2119
Bme18I	GGWCC	4 cut(s)	214, 442, 2325, 2565
BmeRI	GACNNNNNGTC	1 cut(s)	2542
BmeT110I	CYCGRG	2 cut(s)	263, 897
BmgT120I	GGNCC	6 cut(s)	214, 442, 1931, 1932, 2325, 2565
BmiI	GGNNCC	9 cut(s)	60, 216, 261, 443, 1224, 1932, 1933, 2187, 2327
BmrI	ACTGGG	1 cut(s)	2271
BmsI	GCATC	7 cut(s)	91, 463, 977, 1033, 1792, 2101, 2386
BmuI	ACTGGG	1 cut(s)	2271
BpiI	GAAGAC	1 cut(s)	1023
BplI	GAGNNNNNCTC	2 cut(s)	141, 173
BpmI	CTGGAG	6 cut(s)	1241, 1421, 2039, 2082, 2402, 2582
Bpu10I	CCTNAGC	2 cut(s)	591, 1842
BpuEI	CTTGAG	4 cut(s)	410, 681, 1715, 2099
BpuMI	CCSGG	4 cut(s)	264, 265, 1795, 2496
Bsa29I	ATCGAT	1 cut(s)	2312
BsaHI	GRCGYC	2 cut(s)	39, 389
BsaI	GGTCTC	2 cut(s)	76, 2547
BsaJI	CCNNGG	8 cut(s)	62, 192, 263, 264, 324, 875, 876, 2484
BsaWI	WCCGGW	5 cut(s)	345, 886, 1242, 2221, 2894
BsaXI	ACNNNNNCTCC	4 cut(s)	1394, 1424, 2216, 2246
Bsc4I	CCNNNNNNNGG	5 cut(s)	48, 955, 1484, 2197, 2282
Bse118I	RCCGGY	3 cut(s)	1242, 2788, 2894
Bse1I	ACTGG	6 cut(s)	93, 1742, 2099, 2277, 2348, 2434
Bse3DI	GCAATG	3 cut(s)	279, 997, 2148
BseAI	TCCGGA	1 cut(s)	886
BseBI	CCWGG	7 cut(s)	63, 877, 1400, 2183, 2420, 2600, 2835
BseCI	ATCGAT	1 cut(s)	2312
BseDI	CCNNGG	8 cut(s)	62, 192, 263, 264, 324, 875, 876, 2484
BseGI	GGATG	9 cut(s)	85, 514, 855, 907, 1048, 1807, 2092, 2140, 2401
BseLI	CCNNNNNNNGG	5 cut(s)	48, 955, 1484, 2197, 2282
BseMI	GCAATG	3 cut(s)	279, 997, 2148
BseNI	ACTGG	6 cut(s)	93, 1742, 2099, 2277, 2348, 2434
BseRI	GAGGAG	5 cut(s)	364, 675, 1445, 1938, 2645
BseSI	GKGCMC	2 cut(s)	1935, 2128
BsgI	GTGCAG	1 cut(s)	495
Bsh1236I	CGCG	2 cut(s)	194, 1138
Bsh1285I	CGRYCG	1 cut(s)	1267
BshFI	GGCC	6 cut(s)	323, 1488, 1933, 2144, 2440, 2749
BshNI	GGYRCC	1 cut(s)	58
BshTI	ACCGGT	2 cut(s)	1242, 2894
BshVI	ATCGAT	1 cut(s)	2312
BsiEI	CGRYCG	1 cut(s)	1267
BsiHKAI	GWGCWC	3 cut(s)	1150, 1854, 2128
BsiHKCI	CYCGRG	2 cut(s)	263, 897
BsiSI	CCGG	9 cut(s)	264, 346, 887, 1243, 1794, 2222, 2495, 2789, 2895
BslFI	GGGAC	3 cut(s)	281, 439, 455
BslI	CCNNNNNNNGG	5 cut(s)	48, 955, 1484, 2197, 2282
BsmBI	CGTCTC	1 cut(s)	1727
BsmFI	GGGAC	3 cut(s)	281, 439, 455
BsmI	GAATGC	1 cut(s)	2094
BsnI	GGCC	6 cut(s)	323, 1488, 1933, 2144, 2440, 2749
Bso31I	GGTCTC	2 cut(s)	76, 2547
BsoBI	CYCGRG	2 cut(s)	263, 897
Bsp120I	GGGCCC	1 cut(s)	1931
Bsp1286I	GDGCHC	6 cut(s)	1150, 1854, 1935, 2128, 2190, 2620
Bsp13I	TCCGGA	1 cut(s)	886
BspACI	CCGC	3 cut(s)	192, 194, 2795
BspANI	GGCC	6 cut(s)	323, 1488, 1933, 2144, 2440, 2749
BspDI	ATCGAT	1 cut(s)	2312
BspEI	TCCGGA	1 cut(s)	886
BspFNI	CGCG	2 cut(s)	194, 1138
BspHI	TCATGA	2 cut(s)	1183, 1773
BspLI	GGNNCC	9 cut(s)	60, 216, 261, 443, 1224, 1932, 1933, 2187, 2327
BspMAI	CTGCAG	1 cut(s)	1822
BspMI	ACCTGC	1 cut(s)	525
BspQI	GCTCTTC	2 cut(s)	1842, 2625
BspT107I	GGYRCC	1 cut(s)	58
BspTNI	GGTCTC	2 cut(s)	76, 2547
BsrDI	GCAATG	3 cut(s)	279, 997, 2148
BsrFI	RCCGGY	3 cut(s)	1242, 2788, 2894
BsrI	ACTGG	6 cut(s)	93, 1742, 2099, 2277, 2348, 2434
BssAI	RCCGGY	3 cut(s)	1242, 2788, 2894
BssECI	CCNNGG	8 cut(s)	62, 192, 263, 264, 324, 875, 876, 2484
BssNI	GRCGYC	2 cut(s)	39, 389
BssSI	CACGAG	1 cut(s)	1008
BssT1I	CCWWGG	1 cut(s)	2484
Bst2BI	CACGAG	1 cut(s)	1008
Bst2UI	CCWGG	7 cut(s)	63, 877, 1400, 2183, 2420, 2600, 2835
Bst4CI	ACNGT	6 cut(s)	169, 493, 1053, 1215, 1915, 1996
Bst6I	CTCTTC	4 cut(s)	411, 1842, 1915, 2625
BstACI	GRCGYC	2 cut(s)	39, 389
BstAPI	GCANNNNNTGC	1 cut(s)	2089
BstDSI	CCRYGG	1 cut(s)	192
BstF5I	GGATG	9 cut(s)	85, 514, 855, 907, 1048, 1807, 2092, 2140, 2401
BstFNI	CGCG	2 cut(s)	194, 1138
BstH2I	RGCGCY	2 cut(s)	1477, 2590
BstHHI	GCGC	2 cut(s)	1476, 2589
BstMCI	CGRYCG	1 cut(s)	1267
BstNI	CCWGG	7 cut(s)	63, 877, 1400, 2183, 2420, 2600, 2835
BstNSI	RCATGY	4 cut(s)	304, 557, 2384, 2887
BstSFI	CTRYAG	2 cut(s)	492, 1818
BstSLI	GKGCMC	2 cut(s)	1935, 2128
BstUI	CGCG	2 cut(s)	194, 1138
BstV2I	GAAGAC	1 cut(s)	1023
BstX2I	RGATCY	3 cut(s)	259, 958, 2632
BstXI	CCANNNNNNTGG	1 cut(s)	2606
BstYI	RGATCY	3 cut(s)	259, 958, 2632
Bsu15I	ATCGAT	1 cut(s)	2312
BsuRI	GGCC	6 cut(s)	323, 1488, 1933, 2144, 2440, 2749
BsuTUI	ATCGAT	1 cut(s)	2312
BtgI	CCRYGG	1 cut(s)	192
BtgZI	GCGATG	1 cut(s)	1000
BtsCI	GGATG	9 cut(s)	85, 514, 855, 907, 1048, 1807, 2092, 2140, 2401
BtsI	GCAGTG	1 cut(s)	777
BtsIMutI	CAGTG	7 cut(s)	777, 1368, 1655, 2284, 2355, 2441, 2705
BveI	ACCTGC	1 cut(s)	525
CaiI	CAGNNNCTG	1 cut(s)	1219
CciI	TCATGA	2 cut(s)	1183, 1773
CfoI	GCGC	2 cut(s)	1476, 2589
Cfr10I	RCCGGY	3 cut(s)	1242, 2788, 2894
Cfr13I	GGNCC	6 cut(s)	214, 442, 1931, 1932, 2325, 2565
Cfr42I	CCGCGG	1 cut(s)	195
Cfr9I	CCCGGG	1 cut(s)	263
ClaI	ATCGAT	1 cut(s)	2312
CseI	GACGC	4 cut(s)	47, 378, 586, 1424
Csp6I	GTAC	8 cut(s)	97, 343, 663, 752, 1054, 1741, 1783, 2767
CspAI	ACCGGT	2 cut(s)	1242, 2894
CspCI	CAANNNNNGTGG	2 cut(s)	1924, 1959
CviQI	GTAC	8 cut(s)	97, 343, 663, 752, 1054, 1741, 1783, 2767
DraIII	CACNNNGTG	1 cut(s)	2300
DrdI	GACNNNNNNGTC	1 cut(s)	275
DriI	GACNNNNNGTC	1 cut(s)	2542
DseDI	GACNNNNNNGTC	1 cut(s)	275
EaeI	YGGCCR	3 cut(s)	321, 1486, 2142
Eam1104I	CTCTTC	4 cut(s)	411, 1842, 1915, 2625
Eam1105I	GACNNNNNGTC	1 cut(s)	2542
EarI	CTCTTC	4 cut(s)	411, 1842, 1915, 2625
Ecl136II	GAGCTC	1 cut(s)	1852
Eco130I	CCWWGG	1 cut(s)	2484
Eco24I	GRGCYC	4 cut(s)	1854, 1935, 2190, 2620
Eco31I	GGTCTC	2 cut(s)	76, 2547
Eco47I	GGWCC	4 cut(s)	214, 442, 2325, 2565
Eco53kI	GAGCTC	1 cut(s)	1852
Eco57I	CTGAAG	3 cut(s)	119, 1527, 2729
Eco88I	CYCGRG	2 cut(s)	263, 897
EcoICRI	GAGCTC	1 cut(s)	1852
EcoO109I	RGGNCCY	3 cut(s)	214, 442, 1931
EcoRII	CCWGG	7 cut(s)	61, 875, 1398, 2181, 2418, 2598, 2833
EcoT14I	CCWWGG	1 cut(s)	2484
EcoT22I	ATGCAT	1 cut(s)	2094
EcoT38I	GRGCYC	4 cut(s)	1854, 1935, 2190, 2620
ErhI	CCWWGG	1 cut(s)	2484
Esp3I	CGTCTC	1 cut(s)	1727
FalI	AAGNNNNNCTT	2 cut(s)	1434, 1466
FaqI	GGGAC	3 cut(s)	281, 439, 455
FokI	GGATG	9 cut(s)	92, 501, 862, 914, 1055, 1814, 2079, 2147, 2408
FriOI	GRGCYC	4 cut(s)	1854, 1935, 2190, 2620
FspBI	CTAG	3 cut(s)	918, 1340, 2840
GlaI	GCGC	2 cut(s)	1475, 2588
GsuI	CTGGAG	6 cut(s)	1241, 1421, 2039, 2082, 2402, 2582
HaeII	RGCGCY	2 cut(s)	1477, 2590
HaeIII	GGCC	6 cut(s)	323, 1488, 1933, 2144, 2440, 2749
HapII	CCGG	9 cut(s)	264, 346, 887, 1243, 1794, 2222, 2495, 2789, 2895
HgaI	GACGC	4 cut(s)	47, 378, 586, 1424
HhaI	GCGC	2 cut(s)	1476, 2589
Hin1I	GRCGYC	2 cut(s)	39, 389
Hin6I	GCGC	2 cut(s)	1474, 2587
HinP1I	GCGC	2 cut(s)	1474, 2587
HincII	GTYRAC	1 cut(s)	775
HindII	GTYRAC	1 cut(s)	775
HindIII	AAGCTT	4 cut(s)	1124, 1682, 1883, 2519
HpaII	CCGG	9 cut(s)	264, 346, 887, 1243, 1794, 2222, 2495, 2789, 2895
HphI	GGTGA	2 cut(s)	1121, 2015
Hpy166II	GTNNAC	5 cut(s)	752, 775, 1142, 1200, 2126
Hpy8I	GTNNAC	5 cut(s)	752, 775, 1142, 1200, 2126
Hpy99I	CGWCG	3 cut(s)	394, 771, 1440
HpyCH4III	ACNGT	6 cut(s)	169, 493, 1053, 1215, 1915, 1996
HpyCH4IV	ACGT	7 cut(s)	766, 808, 1144, 1196, 1532, 1633, 2688
HpySE526I	ACGT	7 cut(s)	766, 808, 1144, 1196, 1532, 1633, 2688
Hsp92I	GRCGYC	2 cut(s)	39, 389
HspAI	GCGC	2 cut(s)	1474, 2587
Kpn2I	TCCGGA	1 cut(s)	886
KroI	GCCGGC	1 cut(s)	2788
KroNI	GCCGGC	1 cut(s)	2790
KspI	CCGCGG	1 cut(s)	195
LguI	GCTCTTC	2 cut(s)	1842, 2625
LmnI	GCTCC	8 cut(s)	1222, 1259, 1432, 1708, 2185, 2421, 2797, 2836
LweI	GCATC	7 cut(s)	91, 463, 977, 1033, 1792, 2101, 2386
MaeI	CTAG	3 cut(s)	918, 1340, 2840
MaeII	ACGT	7 cut(s)	766, 808, 1144, 1196, 1532, 1633, 2688
MaeIII	GTNAC	6 cut(s)	880, 892, 1238, 1245, 1990, 2452
MfeI	CAATTG	2 cut(s)	33, 2457
MflI	RGATCY	3 cut(s)	259, 958, 2632
MhlI	GDGCHC	6 cut(s)	1150, 1854, 1935, 2128, 2190, 2620
MluI	ACGCGT	1 cut(s)	1136
MlyI	GAGTC	9 cut(s)	163, 263, 413, 415, 676, 1179, 2263, 2460, 2646
MmeI	TCCRAC	6 cut(s)	207, 713, 1897, 2208, 2592, 2840
Mph1103I	ATGCAT	1 cut(s)	2094
MroI	TCCGGA	1 cut(s)	886
MroNI	GCCGGC	1 cut(s)	2788
MroXI	GAANNNNTTC	4 cut(s)	810, 1215, 2205, 2295
MseI	TTAA	6 cut(s)	306, 1881, 2376, 2465, 2685, 2739
MslI	CAYNNNNRTG	1 cut(s)	1371
MspA1I	CMGCKG	2 cut(s)	194, 2795
MspI	CCGG	9 cut(s)	264, 346, 887, 1243, 1794, 2222, 2495, 2789, 2895
MunI	CAATTG	2 cut(s)	33, 2457
Mva1269I	GAATGC	1 cut(s)	2094
MvaI	CCWGG	7 cut(s)	63, 877, 1400, 2183, 2420, 2600, 2835
MvnI	CGCG	2 cut(s)	194, 1138
NaeI	GCCGGC	1 cut(s)	2790
NciI	CCSGG	4 cut(s)	264, 265, 1795, 2496
NgoMIV	GCCGGC	1 cut(s)	2788
NlaIV	GGNNCC	9 cut(s)	60, 216, 261, 443, 1224, 1932, 1933, 2187, 2327
NmeAIII	GCCGAG	1 cut(s)	349
NmuCI	GTSAC	3 cut(s)	880, 1245, 2452
NsiI	ATGCAT	1 cut(s)	2094
NspI	RCATGY	4 cut(s)	304, 557, 2384, 2887
PaeI	GCATGC	3 cut(s)	557, 2384, 2887
PagI	TCATGA	2 cut(s)	1183, 1773
PasI	CCCWGGG	1 cut(s)	876
PciSI	GCTCTTC	2 cut(s)	1842, 2625
PctI	GAATGC	1 cut(s)	2094
PdiI	GCCGGC	1 cut(s)	2790
PdmI	GAANNNNTTC	4 cut(s)	810, 1215, 2205, 2295
PfeI	GAWTC	7 cut(s)	646, 1355, 1790, 2291, 2404, 2666, 2716
PfoI	TCCNGGA	3 cut(s)	1793, 2181, 2418
PinAI	ACCGGT	2 cut(s)	1242, 2894
Ple19I	CGATCG	1 cut(s)	1267
PleI	GAGTC	9 cut(s)	162, 263, 412, 415, 676, 1178, 2262, 2459, 2646
PpsI	GAGTC	9 cut(s)	162, 263, 412, 415, 676, 1178, 2262, 2459, 2646
PpuMI	RGGWCCY	2 cut(s)	214, 442
Psp124BI	GAGCTC	1 cut(s)	1854
Psp1406I	AACGTT	2 cut(s)	1196, 1532
Psp5II	RGGWCCY	2 cut(s)	214, 442
Psp6I	CCWGG	7 cut(s)	61, 875, 1398, 2181, 2418, 2598, 2833
PspGI	CCWGG	7 cut(s)	61, 875, 1398, 2181, 2418, 2598, 2833
PspN4I	GGNNCC	9 cut(s)	60, 216, 261, 443, 1224, 1932, 1933, 2187, 2327
PspOMI	GGGCCC	1 cut(s)	1931
PspPI	GGNCC	6 cut(s)	214, 442, 1931, 1932, 2325, 2565
PspPPI	RGGWCCY	2 cut(s)	214, 442
PsrI	GAACNNNNNNTAC	2 cut(s)	486, 518
PstI	CTGCAG	1 cut(s)	1822
PstNI	CAGNNNCTG	1 cut(s)	1219
PsuI	RGATCY	3 cut(s)	259, 958, 2632
PvuI	CGATCG	1 cut(s)	1267
RsaI	GTAC	8 cut(s)	98, 344, 664, 753, 1055, 1742, 1784, 2768
RsaNI	GTAC	8 cut(s)	97, 343, 663, 752, 1054, 1741, 1783, 2767
RseI	CAYNNNNRTG	1 cut(s)	1371
SacI	GAGCTC	1 cut(s)	1854
SacII	CCGCGG	1 cut(s)	195
SapI	GCTCTTC	2 cut(s)	1842, 2625
SaqAI	TTAA	6 cut(s)	306, 1881, 2376, 2465, 2685, 2739
Sau96I	GGNCC	6 cut(s)	214, 442, 1931, 1932, 2325, 2565
SchI	GAGTC	9 cut(s)	163, 263, 413, 415, 676, 1179, 2263, 2460, 2646
SduI	GDGCHC	6 cut(s)	1150, 1854, 1935, 2128, 2190, 2620
SfaNI	GCATC	7 cut(s)	91, 463, 977, 1033, 1792, 2101, 2386
SfcI	CTRYAG	2 cut(s)	492, 1818
Sfr303I	CCGCGG	1 cut(s)	195
SgrBI	CCGCGG	1 cut(s)	195
SinI	GGWCC	4 cut(s)	214, 442, 2325, 2565
SmaI	CCCGGG	1 cut(s)	265
SmiMI	CAYNNNNRTG	1 cut(s)	1371
SmlI	CTYRAG	4 cut(s)	425, 696, 1694, 2078
SmoI	CTYRAG	4 cut(s)	425, 696, 1694, 2078
SphI	GCATGC	3 cut(s)	557, 2384, 2887
SsiI	CCGC	3 cut(s)	192, 194, 2795
SspMI	CTAG	3 cut(s)	918, 1340, 2840
SstI	GAGCTC	1 cut(s)	1854
StyI	CCWWGG	1 cut(s)	2484
TaaI	ACNGT	6 cut(s)	169, 493, 1053, 1215, 1915, 1996
TaiI	ACGT	7 cut(s)	769, 811, 1147, 1199, 1535, 1636, 2691
TaqI	TCGA	7 cut(s)	624, 1267, 1417, 1903, 2239, 2312, 2490
TaqII	GACCGA	1 cut(s)	2571
TatI	WGTACW	2 cut(s)	1053, 2766
TfiI	GAWTC	7 cut(s)	646, 1355, 1790, 2291, 2404, 2666, 2716
Tru1I	TTAA	6 cut(s)	306, 1881, 2376, 2465, 2685, 2739
Tru9I	TTAA	6 cut(s)	306, 1881, 2376, 2465, 2685, 2739
TscAI	CASTG	7 cut(s)	784, 1375, 1662, 2284, 2355, 2441, 2712
TseFI	GTSAC	3 cut(s)	880, 1245, 2452
Tsp45I	GTSAC	3 cut(s)	880, 1245, 2452
TspGWI	ACGGA	1 cut(s)	416
TspMI	CCCGGG	1 cut(s)	263
TspRI	CASTG	7 cut(s)	784, 1375, 1662, 2284, 2355, 2441, 2712
VneI	GTGCAC	1 cut(s)	2124
VpaK11BI	GGWCC	4 cut(s)	214, 442, 2325, 2565
XapI	RAATTY	3 cut(s)	316, 758, 1074
XbaI	TCTAGA	1 cut(s)	2839
XceI	RCATGY	4 cut(s)	304, 557, 2384, 2887
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2115
XmaI	CCCGGG	1 cut(s)	263
XmnI	GAANNNNTTC	4 cut(s)	810, 1215, 2205, 2295
XspI	CTAG	3 cut(s)	918, 1340, 2840
Zsp2I	ATGCAT	1 cut(s)	2094

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.