pycom09g16490

UBA-like domain (DUF1421)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0010117
Physical Location & Seq
Reverse (-)
16777221 .. 16780508
3288 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1710 bp
ATGGGGAATAATCCCGGGAAGGATTTTCATAAAAGTAGAATGTCGAGACAATCAATGTTCTCTTCTCCTGCCTATAGCCAGCCGGAAGAATCCCTTCACCAAGATGTAATTGCTACTGTCGAAAAGAGCATGAAAAAGTATGCCGACAATCTCATGCGCTTTCTTGAAGGAATTAGTTCACGGCTTTCGCAGCTGGAATTGTATTGCTACAATCTTGATAAGTCAATTGGTGAGATGCGATCTGATTTAGGTCGTGATCATGAAGAAGCAGATTCAAAGCTCAAGTCTCTTGAGAAACATCTTCAAGAAGTCCATAGGTCTGTCCAGATCCTAAGGGACAAGCAAGAACTTGCGGAGACTCAGAAAGAATTAGCTAAGCTTCAGCTTGCACAGAAAGGATCTGGATCATCAATCCATTCCCAATCCAATGAGGAGAGAGCTTCACCACATACCTCTGATGCTCAAAAGGCTGACAACCCGTCTGAAACACATAACCAGCAATTAGCTCTAGCCCTGCCCCATCAAGTGGCACCACAGCAGCAGCCTGTGGCAGCTCCCACGCAGCCCCCACCTCAGAATGTGACTCAGCAACAATCATATTATTTACCAACCCAGCTACAAAATCCACCACCTCAAGCACATCCGCAGAATCAATATTTGCCTCCTGATTCTCAGTATCGAAACCCCCAAGTGCAAGAAGTGCAAAGGGTGACACCACAGCCAACACAATCGCAATTGAATCAGACACAAGTCCAGTCGTTCCCTCAGTATCAGCAGCAATTACCACAGAGTCAGCAGGTGCAATTTCCACAACAGCCCTCTATGCAACCCCAGATGAGGCCACCACCAACAAATGTCTACCCCTATCCGCCTGGTCAACAAACAAACCCATCTCCTCCAGAGTCTGCACCAAACAGCATGCCGATGCAAGTGCCATATTCCAACATTCCTCAACCAGGATCTGGTCGTTCTGATCCCATGCCTTTTGGATATGGTGGAGCTGGTCGAACAATGCAACAGCAGCCTCCACCTCAGCAAATCAAGGGGGCTTTTGGAGCTCAACCTGGTGAGGGATATGGTGCTGCTGGCCCTCACCCTGCACTTCCTCCTGGGAGTACATATATGGTCTATGACAGTGAAGGGGGTAGAACTCAATATTCTGCCCATTATGGATATCCTCCTACGAGTATCTCTCACCATACGCCACAGCCAACAACAGCTCCAAATCTTATGGCTCGGAATCCACAATTCCCGCACAACCACCCTTACAATGAGTTGATTGAGAAATTGGTTCCCATGGGGTTCAGGAGTGATCACGTTGCGAGTGTGATTCAGAGGATGGAAGAAAGCGGTCAACCCGTTGATTTTAATGCAGTGATTGACAGGTTGAATGCACATCCATCTGGGGGTCCTCACAGAGGATGGTCAGGGTTTGTGGCCGGCAGCGTTCGGTGTCTGCGCCGAAGTATAGATAGTATCTTATGGCTTTTGACTTTGATTGCTTGTGATAGAGTTGTTATATCTTTATTGCCATGCTTGTTCTTTACTGTGTTGATTGCGAGCTTTGCTCCCACAATCGCTCTATGCAATCTTTTCCGACTCCGACAAATCTCCTACCTCCCGCTGCCATCTCTCCCTCTCTTGGGTGCAACCTACGATGACACTGCCCTCACCAGCCTCCGCCCGGTTGTCCGCCGTATATCGGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1421

Protein Analysis

570

Amino Acids

63.19

Weight (kDa)

7.75

Isoelectric Point (pI)

67.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1421 PF07223 423 - 466 3.1e-22 UBA-like domain (DUF1421)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 787
Acc36I ACCTGC 1 cut(s) 787
AccB1I GGYRCC 1 cut(s) 529
AccB7I CCANNNNNTGG 2 cut(s) 526, 962
AccI GTMKAC 1 cut(s) 858
AciI CCGC 8 cut(s) 353, 644, 869, 1253, 1350, 1622, 1681, 1693
AclWI GGATC 5 cut(s) 322, 406, 412, 967, 968
AcoI YGGCCR 1 cut(s) 1437
AcuI CTGAAG 1 cut(s) 365
AfaI GTAC 1 cut(s) 1117
AgsI TTSAA 5 cut(s) 167, 276, 305, 739, 1390
AhdI GACNNNNNGTC 1 cut(s) 478
AjnI CCWGG 4 cut(s) 871, 955, 1063, 1108
Alw21I GWGCWC 1 cut(s) 1060
Alw26I GTCTC 3 cut(s) 40, 291, 350
AlwI GGATC 5 cut(s) 322, 406, 412, 967, 968
AlwNI CAGNNNCTG 2 cut(s) 905, 962
Ama87I CYCGRG 1 cut(s) 14
AoxI GGCC 3 cut(s) 839, 1087, 1437
ArsI GACNNNNNNTTYG 2 cut(s) 269, 301
Asp700I GAANNNNTTC 2 cut(s) 93, 175
AspLEI GCGC 2 cut(s) 159, 1461
AspS9I GGNCC 2 cut(s) 1088, 1409
AsuC2I CCSGG 3 cut(s) 15, 16, 1685
AsuHPI GGTGA 8 cut(s) 89, 242, 435, 721, 1079, 1085, 1187, 1663
AvaI CYCGRG 1 cut(s) 14
AvaII GGWCC 1 cut(s) 1409
AxyI CCTNAGG 1 cut(s) 332
BanI GGYRCC 1 cut(s) 529
BanII GRGCYC 1 cut(s) 1060
Bbv12I GWGCWC 1 cut(s) 1060
BbvCI CCTCAGC 1 cut(s) 1032
BccI CCATC 6 cut(s) 528, 898, 1333, 1408, 1416, 1636
BceAI ACGGC 2 cut(s) 197, 1680
BcgI CGANNNNNNTGC 4 cut(s) 913, 947, 1646, 1680
BciT130I CCWGG 4 cut(s) 873, 957, 1065, 1110
BclI TGATCA 2 cut(s) 256, 1312
BcnI CCSGG 3 cut(s) 15, 16, 1685
BcoDI GTCTC 3 cut(s) 40, 291, 350
BfaI CTAG 1 cut(s) 509
BfmI CTRYAG 1 cut(s) 73
BfuAI ACCTGC 1 cut(s) 787
BlpI GCTNAGC 1 cut(s) 375
Bme1390I CCNGG 7 cut(s) 15, 16, 873, 957, 1065, 1110, 1685
Bme18I GGWCC 1 cut(s) 1409
BmeRI GACNNNNNGTC 1 cut(s) 478
BmeT110I CYCGRG 1 cut(s) 14
BmgT120I GGNCC 2 cut(s) 1088, 1409
BmiI GGNNCC 3 cut(s) 531, 1293, 1410
BmrFI CCNGG 7 cut(s) 15, 16, 873, 957, 1065, 1110, 1685
BmsI GCATC 3 cut(s) 225, 448, 915
BoxI GACNNNNGTC 1 cut(s) 749
BplI GAGNNNNNCTC 4 cut(s) 1177, 1209, 1552, 1584
BpmI CTGGAG 1 cut(s) 882
Bpu10I CCTNAGC 1 cut(s) 1032
Bpu1102I GCTNAGC 1 cut(s) 375
BpuEI CTTGAG 3 cut(s) 266, 311, 618
BpuMI CCSGG 3 cut(s) 15, 16, 1685
BsaBI GATNNNNATC 1 cut(s) 403
BsaJI CCNNGG 3 cut(s) 14, 1109, 1296
BsaXI ACNNNNNCTCC 2 cut(s) 1204, 1234
Bse118I RCCGGY 1 cut(s) 1439
Bse1I ACTGG 1 cut(s) 754
Bse21I CCTNAGG 1 cut(s) 332
Bse8I GATNNNNATC 1 cut(s) 403
BseBI CCWGG 4 cut(s) 873, 957, 1065, 1110
BseDI CCNNGG 3 cut(s) 14, 1109, 1296
BseGI GGATG 4 cut(s) 640, 1344, 1396, 1427
BseJI GATNNNNATC 1 cut(s) 403
BseMII CTCAG 6 cut(s) 374, 587, 599, 686, 779, 1046
BseNI ACTGG 1 cut(s) 754
BseRI GAGGAG 2 cut(s) 446, 885
BseYI CCCAGC 1 cut(s) 612
BsgI GTGCAG 2 cut(s) 891, 1083
BshFI GGCC 3 cut(s) 841, 1089, 1439
BshNI GGYRCC 1 cut(s) 529
BsiHKAI GWGCWC 1 cut(s) 1060
BsiHKCI CYCGRG 1 cut(s) 14
BsiSI CCGG 4 cut(s) 15, 83, 1440, 1685
BslFI GGGAC 1 cut(s) 350
BsmAI GTCTC 3 cut(s) 40, 291, 350
BsmFI GGGAC 1 cut(s) 350
BsmI GAATGC 1 cut(s) 1396
BsnI GGCC 3 cut(s) 841, 1089, 1439
BsoBI CYCGRG 1 cut(s) 14
Bsp1286I GDGCHC 1 cut(s) 1060
Bsp143I GATC 8 cut(s) 239, 256, 327, 398, 404, 959, 973, 1312
Bsp1720I GCTNAGC 1 cut(s) 375
Bsp19I CCATGG 1 cut(s) 1296
BspACI CCGC 8 cut(s) 353, 644, 869, 1253, 1350, 1622, 1681, 1693
BspANI GGCC 3 cut(s) 841, 1089, 1439
BspCNI CTCAG 6 cut(s) 373, 586, 598, 685, 778, 1045
BspHI TCATGA 1 cut(s) 259
BspLI GGNNCC 3 cut(s) 531, 1293, 1410
BspMI ACCTGC 1 cut(s) 787
BspPI GGATC 5 cut(s) 322, 406, 412, 967, 968
BspT107I GGYRCC 1 cut(s) 529
BsrFI RCCGGY 1 cut(s) 1439
BsrI ACTGG 1 cut(s) 754
BssAI RCCGGY 1 cut(s) 1439
BssECI CCNNGG 3 cut(s) 14, 1109, 1296
BssMI GATC 8 cut(s) 239, 256, 327, 398, 404, 959, 973, 1312
BssT1I CCWWGG 1 cut(s) 1296
Bst2UI CCWGG 4 cut(s) 873, 957, 1065, 1110
Bst4CI ACNGT 3 cut(s) 118, 1136, 1549
Bst6I CTCTTC 1 cut(s) 67
BstAPI GCANNNNNTGC 1 cut(s) 700
BstC8I GCNNGC 6 cut(s) 80, 387, 920, 1087, 1441, 1561
BstDEI CTNAG 8 cut(s) 332, 360, 375, 573, 585, 672, 765, 1032
BstDSI CCRYGG 1 cut(s) 1296
BstENI CCTNNNNNAGG 2 cut(s) 954, 1416
BstF5I GGATG 4 cut(s) 640, 1344, 1396, 1427
BstHHI GCGC 2 cut(s) 159, 1461
BstKTI GATC 8 cut(s) 242, 259, 330, 401, 407, 962, 976, 1315
BstMAI GTCTC 3 cut(s) 40, 291, 350
BstMBI GATC 8 cut(s) 239, 256, 327, 398, 404, 959, 973, 1312
BstMWI GCNNNNNNNGC 7 cut(s) 190, 467, 700, 823, 1021, 1055, 1565
BstNI CCWGG 4 cut(s) 873, 957, 1065, 1110
BstNSI RCATGY 1 cut(s) 922
BstPAI GACNNNNGTC 1 cut(s) 749
BstSCI CCNGG 7 cut(s) 13, 14, 871, 955, 1063, 1108, 1683
BstSFI CTRYAG 1 cut(s) 73
BstX2I RGATCY 3 cut(s) 327, 398, 959
BstYI RGATCY 3 cut(s) 327, 398, 959
Bsu36I CCTNAGG 1 cut(s) 332
BsuRI GGCC 3 cut(s) 841, 1089, 1439
BtgI CCRYGG 1 cut(s) 1296
BtsCI GGATG 4 cut(s) 640, 1344, 1396, 1427
BtsI GCAGTG 2 cut(s) 1380, 1662
BtsIMutI CAGTG 3 cut(s) 1141, 1380, 1662
BveI ACCTGC 1 cut(s) 787
Cac8I GCNNGC 6 cut(s) 80, 387, 920, 1087, 1441, 1561
CaiI CAGNNNCTG 2 cut(s) 905, 962
CciI TCATGA 1 cut(s) 259
CfoI GCGC 2 cut(s) 159, 1461
Cfr10I RCCGGY 1 cut(s) 1439
Cfr13I GGNCC 2 cut(s) 1088, 1409
Cfr9I CCCGGG 1 cut(s) 14
CsiI ACCWGGT 1 cut(s) 1063
Csp6I GTAC 1 cut(s) 1116
CviAII CATG 7 cut(s) 130, 154, 260, 919, 979, 1297, 1533
CviQI GTAC 1 cut(s) 1116
DdeI CTNAG 8 cut(s) 332, 360, 375, 573, 585, 672, 765, 1032
DpnI GATC 8 cut(s) 241, 258, 329, 400, 406, 961, 975, 1314
DpnII GATC 8 cut(s) 239, 256, 327, 398, 404, 959, 973, 1312
DriI GACNNNNNGTC 1 cut(s) 478
EaeI YGGCCR 1 cut(s) 1437
Eam1104I CTCTTC 1 cut(s) 67
Eam1105I GACNNNNNGTC 1 cut(s) 478
EarI CTCTTC 1 cut(s) 67
EciI GGCGGA 3 cut(s) 858, 1670, 1682
Ecl136II GAGCTC 1 cut(s) 1058
Eco130I CCWWGG 1 cut(s) 1296
Eco24I GRGCYC 1 cut(s) 1060
Eco32I GATATC 1 cut(s) 1175
Eco47I GGWCC 1 cut(s) 1409
Eco53kI GAGCTC 1 cut(s) 1058
Eco57I CTGAAG 1 cut(s) 365
Eco81I CCTNAGG 1 cut(s) 332
Eco88I CYCGRG 1 cut(s) 14
EcoICRI GAGCTC 1 cut(s) 1058
EcoNI CCTNNNNNAGG 2 cut(s) 954, 1416
EcoO109I RGGNCCY 1 cut(s) 1409
EcoRII CCWGG 4 cut(s) 871, 955, 1063, 1108
EcoRV GATATC 1 cut(s) 1175
EcoT14I CCWWGG 1 cut(s) 1296
EcoT38I GRGCYC 1 cut(s) 1060
ErhI CCWWGG 1 cut(s) 1296
FaeI CATG 7 cut(s) 133, 157, 263, 922, 982, 1300, 1536
FalI AAGNNNNNCTT 2 cut(s) 78, 110
FaqI GGGAC 1 cut(s) 350
FatI CATG 7 cut(s) 129, 153, 259, 918, 978, 1296, 1532
FauI CCCGC 2 cut(s) 1260, 1629
FbaI TGATCA 2 cut(s) 256, 1312
FblI GTMKAC 1 cut(s) 858
FokI GGATG 4 cut(s) 627, 1351, 1383, 1434
FriOI GRGCYC 1 cut(s) 1060
FspBI CTAG 1 cut(s) 509
GlaI GCGC 2 cut(s) 158, 1460
GsaI CCCAGC 1 cut(s) 616
GsuI CTGGAG 1 cut(s) 882
HaeIII GGCC 3 cut(s) 841, 1089, 1439
HapII CCGG 4 cut(s) 15, 83, 1440, 1685
HhaI GCGC 2 cut(s) 159, 1461
Hin1II CATG 7 cut(s) 133, 157, 263, 922, 982, 1300, 1536
Hin6I GCGC 2 cut(s) 157, 1459
HinP1I GCGC 2 cut(s) 157, 1459
HincII GTYRAC 2 cut(s) 878, 1355
HindII GTYRAC 2 cut(s) 878, 1355
HindIII AAGCTT 1 cut(s) 377
HpaII CCGG 4 cut(s) 15, 83, 1440, 1685
HphI GGTGA 8 cut(s) 89, 242, 435, 721, 1079, 1085, 1187, 1663
Hpy166II GTNNAC 4 cut(s) 179, 859, 878, 1355
Hpy8I GTNNAC 4 cut(s) 179, 859, 878, 1355
HpyAV CCTTC 4 cut(s) 13, 104, 161, 1133
HpyCH4III ACNGT 3 cut(s) 118, 1136, 1549
HpyCH4IV ACGT 1 cut(s) 1317
HpyF10VI GCNNNNNNNGC 7 cut(s) 190, 467, 700, 823, 1021, 1055, 1565
HpyF3I CTNAG 8 cut(s) 332, 360, 375, 573, 585, 672, 765, 1032
HpySE526I ACGT 1 cut(s) 1317
Hsp92II CATG 7 cut(s) 133, 157, 263, 922, 982, 1300, 1536
HspAI GCGC 2 cut(s) 157, 1459
KroI GCCGGC 1 cut(s) 1439
KroNI GCCGGC 1 cut(s) 1441
Ksp22I TGATCA 2 cut(s) 256, 1312
Kzo9I GATC 8 cut(s) 239, 256, 327, 398, 404, 959, 973, 1312
LmnI GCTCC 5 cut(s) 559, 998, 1055, 1225, 1573
LweI GCATC 3 cut(s) 225, 448, 915
MabI ACCWGGT 1 cut(s) 1063
MaeI CTAG 1 cut(s) 509
MaeII ACGT 1 cut(s) 1317
MaeIII GTNAC 2 cut(s) 580, 709
MalI GATC 8 cut(s) 241, 258, 329, 400, 406, 961, 975, 1314
MboI GATC 8 cut(s) 239, 256, 327, 398, 404, 959, 973, 1312
MboII GAAGA 5 cut(s) 54, 98, 275, 293, 1355
MfeI CAATTG 2 cut(s) 225, 734
MflI RGATCY 3 cut(s) 327, 398, 959
MhlI GDGCHC 1 cut(s) 1060
MlyI GAGTC 5 cut(s) 352, 577, 799, 911, 1593
MmeI TCCRAC 3 cut(s) 966, 1621, 1627
MroNI GCCGGC 1 cut(s) 1439
MroXI GAANNNNTTC 2 cut(s) 93, 175
MseI TTAA 1 cut(s) 1368
MslI CAYNNNNRTG 2 cut(s) 102, 923
MspA1I CMGCKG 2 cut(s) 193, 1624
MspI CCGG 4 cut(s) 15, 83, 1440, 1685
MspR9I CCNGG 7 cut(s) 15, 16, 873, 957, 1065, 1110, 1685
MunI CAATTG 2 cut(s) 225, 734
Mva1269I GAATGC 1 cut(s) 1396
MvaI CCWGG 4 cut(s) 873, 957, 1065, 1110
MwoI GCNNNNNNNGC 7 cut(s) 190, 467, 700, 823, 1021, 1055, 1565
NaeI GCCGGC 1 cut(s) 1441
NciI CCSGG 3 cut(s) 15, 16, 1685
NcoI CCATGG 1 cut(s) 1296
NdeII GATC 8 cut(s) 239, 256, 327, 398, 404, 959, 973, 1312
NgoMIV GCCGGC 1 cut(s) 1439
NlaIII CATG 7 cut(s) 133, 157, 263, 922, 982, 1300, 1536
NlaIV GGNNCC 3 cut(s) 531, 1293, 1410
NmuCI GTSAC 2 cut(s) 580, 709
NspI RCATGY 1 cut(s) 922
PaeI GCATGC 1 cut(s) 922
PagI TCATGA 1 cut(s) 259
PaqCI CACCTGC 1 cut(s) 787
PctI GAATGC 1 cut(s) 1396
PdiI GCCGGC 1 cut(s) 1441
PdmI GAANNNNTTC 2 cut(s) 93, 175
PfeI GAWTC 7 cut(s) 89, 272, 649, 668, 739, 1240, 1330
PflMI CCANNNNNTGG 2 cut(s) 526, 962
PleI GAGTC 5 cut(s) 352, 577, 798, 910, 1593
PpsI GAGTC 5 cut(s) 352, 577, 798, 910, 1593
PpuMI RGGWCCY 1 cut(s) 1409
PshAI GACNNNNGTC 1 cut(s) 749
Psp124BI GAGCTC 1 cut(s) 1060
Psp5II RGGWCCY 1 cut(s) 1409
Psp6I CCWGG 4 cut(s) 871, 955, 1063, 1108
PspFI CCCAGC 1 cut(s) 612
PspGI CCWGG 4 cut(s) 871, 955, 1063, 1108
PspN4I GGNNCC 3 cut(s) 531, 1293, 1410
PspPI GGNCC 2 cut(s) 1088, 1409
PspPPI RGGWCCY 1 cut(s) 1409
PstNI CAGNNNCTG 2 cut(s) 905, 962
PsuI RGATCY 3 cut(s) 327, 398, 959
PvuII CAGCTG 1 cut(s) 193
RsaI GTAC 1 cut(s) 1117
RsaNI GTAC 1 cut(s) 1116
RseI CAYNNNNRTG 2 cut(s) 102, 923
SacI GAGCTC 1 cut(s) 1060
SaqAI TTAA 1 cut(s) 1368
Sau3AI GATC 8 cut(s) 239, 256, 327, 398, 404, 959, 973, 1312
Sau96I GGNCC 2 cut(s) 1088, 1409
SchI GAGTC 5 cut(s) 352, 577, 799, 911, 1593
ScrFI CCNGG 7 cut(s) 15, 16, 873, 957, 1065, 1110, 1685
SduI GDGCHC 1 cut(s) 1060
SexAI ACCWGGT 1 cut(s) 1063
SfaNI GCATC 3 cut(s) 225, 448, 915
SfcI CTRYAG 1 cut(s) 73
SinI GGWCC 1 cut(s) 1409
SmaI CCCGGG 1 cut(s) 16
SmiMI CAYNNNNRTG 2 cut(s) 102, 923
SmlI CTYRAG 3 cut(s) 281, 290, 633
SmoI CTYRAG 3 cut(s) 281, 290, 633
SphI GCATGC 1 cut(s) 922
SsiI CCGC 8 cut(s) 353, 644, 869, 1253, 1350, 1622, 1681, 1693
SspI AATATT 2 cut(s) 656, 1157
SspMI CTAG 1 cut(s) 509
SstI GAGCTC 1 cut(s) 1060
StyD4I CCNGG 7 cut(s) 13, 14, 871, 955, 1063, 1108, 1683
StyI CCWWGG 1 cut(s) 1296
TaaI ACNGT 3 cut(s) 118, 1136, 1549
TaiI ACGT 1 cut(s) 1320
TaqI TCGA 4 cut(s) 44, 120, 679, 1006
TatI WGTACW 1 cut(s) 1115
TfiI GAWTC 7 cut(s) 89, 272, 649, 668, 739, 1240, 1330
Tru1I TTAA 1 cut(s) 1368
Tru9I TTAA 1 cut(s) 1368
TscAI CASTG 3 cut(s) 1141, 1380, 1669
TseFI GTSAC 2 cut(s) 580, 709
Tsp45I GTSAC 2 cut(s) 580, 709
TspDTI ATGAA 3 cut(s) 17, 146, 276
TspMI CCCGGG 1 cut(s) 14
TspRI CASTG 3 cut(s) 1141, 1380, 1669
Van91I CCANNNNNTGG 2 cut(s) 526, 962
VpaK11BI GGWCC 1 cut(s) 1409
XagI CCTNNNNNAGG 2 cut(s) 954, 1416
XceI RCATGY 1 cut(s) 922
XmaI CCCGGG 1 cut(s) 14
XmiI GTMKAC 1 cut(s) 858
XmnI GAANNNNTTC 2 cut(s) 93, 175
XspI CTAG 1 cut(s) 509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.