pycom10g01270

actinin binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0004156
Physical Location & Seq
Reverse (-)
1344920 .. 1346859
1940 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g01270.2

Sequence Viewer

Length: 1821 bp
ATGGAAGTAAGGAATGCAATCATTCCAATATTCTATGGTGTAGAGCCCTCCCTTGTTCGAGAGCAAAAAGGAACTTTCGAAGCGGCCTTTGACGAGCATGAAAGAATTAGCGAGGACATGAGAAGGGTGCAAGAATGGAGACGTGCTTTACAGAAAGTGAGCGTTATAGCAGGGATGAAGTCAGATGACTATAGTTATGAGACTGAGCTTATCAACGAGATTGTCAAAATAGTGTGTAACAAAGTGCATCCCACTTTCAAATTGTTAGGTTCTGCAAAGAAGATGGCGGGAATTGATTTTAGACTGAAAAAATTACATTTGCTTTTGGATCTAGAAGCAAACGATGTTCGTTTTATAGGCATATGTGGGAAAGCCGGCATAGGTAAGACGCTCACTGCTAGACAATTTTATGACGAATTTTCCCATTATTTTGAAGTCAGTTGTTTTCTTGCTGATGTTGGAACGGTTTCTGCAACATACGGTCTCCTTCATCTGCAAAATCAGCTTCTTTCCTCTGTTCTGAAAACCAATACACAAGTTGAGGACGATTATGCTGGTACCGTAATGACAAAGAAATGTTTTAATAATAAAAAGGTTCTTCTCGTTCTTGATGGTGTGGATCAGTTAGGCCAACTAGCAAGAGTGGCTGGAAATAAAAACTGGTTTGGTCTGGGTAGCAGAATCATCGTTACAACAAGGGATGAACGTCTGTTAGAGGAGCTAAGCTTGAACTTGGATTCTTGTCCACCATGTGGCAATAATGATGGATTCAGGGTATGTGCTGGCTGCAACAGGAATATTCGTGGGCGGTATTTAGGATGCATTGGAGCTTTTTGGCATCCTGAATGTTTTGTTTGCATCACTTGTAATCTTCCAATTACAGATCTTGAGTTTTCTGCTCCTAGAGATGACAGTCACAGCCCTTACCACATATCCTGCTATAAGGCGCAGCACCATCCAAACTGTGATGTTTGCAAGACTTTTATCCCTACTAGAAAATCAGACGGTCGTATTGAGTATAGGCAACATCCTTTCTGGCTACAAAAATACTGCCCCTCTCATGAGCGCGACGGAACTCCTAGGTGTTGTGGCTGTCAAAGAATGGAGCCATGGGCGACCAAATATTTTTTACTTCCTGATGGTCGGAAGCAATGCTCAGAGTGTAATGACTCCGAAATTATGGATGATCACAAGTGCAATTCCCTTTTTCTTCAAGTACAAGCCCTCTTTGAAGATTTAGGTATGAAATTTGAGCAGCAAATCCAAGTAATCTTGGTTGGGAGACGGACATTAAACAAGGACACCGAGGTAGAAAGGCTCGGTCATCACGACCTGTCAGAGACTAAAGCAGTTTTCTCCCCTTCGGCAGAACCTATCGTTACTAGTATTCTAAGGATGCTGAAAGGACACAGTAGAATAACATCAATTCAGATGCTGTATGGCCTTCCTAGGTTGCTTTCAGGGTTAATTCTGGCTGCAATGATGATGTCCGCATGGCTAGGGTTTAATGGTTATCGGAATCTGAGCCTGGAGGTTGAATTTGGTATCCGCCAAGTGTTGGCCCATATGTGGCTGGTCTCTCAGACGTCTTCTGGGTCTAGTTCGACAATGTCTAATGAAGAGAAGAAGCTTGGTGAATTTTTTAAACATTCAATTGAGACAGATACAACAATTTCCGGGCGAGGATTCAGGACAGGAAATCAAGCAGTGCGGAAGCATGGTCTATGGAGGACTCTTGACCATATTCGAACCACAGGAGCTTTCCCCATTGACTGTAGAGACAGCGACATATCTGAATTCTTGTCAGATGTACTTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000131 GO:0000278 GO:0000281 GO:0000746 GO:0000747 GO:0000755 GO:0000910 GO:0000917 GO:0003674 GO:0005096 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005933 GO:0005934 GO:0005935 GO:0005937 GO:0006109 GO:0006996 GO:0007010 GO:0007049 GO:0008047 GO:0008150 GO:0008360 GO:0009272 GO:0009889 GO:0009966 GO:0009968 GO:0009987 GO:0010556 GO:0010646 GO:0010648 GO:0010675 GO:0010962 GO:0010981 GO:0016043 GO:0019222 GO:0019904 GO:0019953 GO:0022402 GO:0022413 GO:0022414 GO:0022603 GO:0022604 GO:0022607 GO:0023051 GO:0023057 GO:0030016 GO:0030017 GO:0030029 GO:0030036 GO:0030234 GO:0030427 GO:0030695 GO:0031323 GO:0031326 GO:0031430 GO:0031672 GO:0032505 GO:0032506 GO:0032881 GO:0032885 GO:0032950 GO:0032951 GO:0032952 GO:0032953 GO:0032995 GO:0035023 GO:0035024 GO:0042546 GO:0042995 GO:0043085 GO:0043087 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043255 GO:0043292 GO:0043332 GO:0043547 GO:0044085 GO:0044087 GO:0044093 GO:0044422 GO:0044424 GO:0044444 GO:0044449 GO:0044463 GO:0044464 GO:0044703 GO:0044764 GO:0046578 GO:0046580 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0051056 GO:0051058 GO:0051128 GO:0051179 GO:0051286 GO:0051301 GO:0051336 GO:0051345 GO:0051641 GO:0051666 GO:0051704 GO:0055120 GO:0060237 GO:0060255 GO:0060589 GO:0061640 GO:0065007 GO:0065008 GO:0065009 GO:0071554 GO:0071840 GO:0071852 GO:0080090 GO:0090036 GO:0090038 GO:0090334 GO:0090529 GO:0098772 GO:0099080 GO:0099081 GO:0099512 GO:0120025 GO:0120038 GO:1902410 GO:1902531 GO:1902532 GO:1903047 GO:1903338 GO:2000112
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DA1-like LIM RhoGAP UIM

Protein Analysis

607

Amino Acids

68.76

Weight (kDa)

7.26

Isoelectric Point (pI)

37.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1589
Acc65I GGTACC 1 cut(s) 557
AccB1I GGYRCC 1 cut(s) 557
AccB7I CCANNNNNTGG 2 cut(s) 752, 1558
AccII CGCG 1 cut(s) 1068
AciI CCGC 6 cut(s) 83, 287, 808, 1491, 1549, 1711
AclWI GGATC 2 cut(s) 336, 627
AcsI RAATTY 5 cut(s) 416, 1247, 1538, 1637, 1796
AcyI GRCGYC 1 cut(s) 1586
AdeI CACNNNGTG 1 cut(s) 752
AfaI GTAC 3 cut(s) 559, 1218, 1812
AfiI CCNNNNNNNGG 3 cut(s) 752, 1558, 1569
AgsI TTSAA 7 cut(s) 259, 434, 730, 1214, 1232, 1538, 1653
AhlI ACTAGT 1 cut(s) 1382
AjiI CACGTC 1 cut(s) 143
AjnI CCWGG 1 cut(s) 1527
AluBI AGCT 7 cut(s) 208, 505, 721, 726, 830, 1630, 1760
AluI AGCT 7 cut(s) 208, 505, 721, 726, 830, 1630, 1760
Alw26I GTCTC 8 cut(s) 133, 194, 488, 1276, 1334, 1582, 1652, 1773
AlwI GGATC 2 cut(s) 336, 627
AlwNI CAGNNNCTG 1 cut(s) 1435
AoxI GGCC 4 cut(s) 84, 628, 1441, 1560
ApeKI GCWGC 4 cut(s) 786, 949, 1255, 1475
ApoI RAATTY 5 cut(s) 416, 1247, 1538, 1637, 1796
Asp700I GAANNNNTTC 1 cut(s) 466
Asp718I GGTACC 1 cut(s) 557
AspA2I CCTAGG 2 cut(s) 1079, 1448
AspLEI GCGC 2 cut(s) 949, 1068
AspS9I GGNCC 1 cut(s) 1561
AsuC2I CCSGG 1 cut(s) 1678
AsuHPI GGTGA 1 cut(s) 1646
AsuII TTCGAA 2 cut(s) 78, 1747
AvrII CCTAGG 2 cut(s) 1079, 1448
BanI GGYRCC 1 cut(s) 557
BanII GRGCYC 1 cut(s) 48
BbsI GAAGAC 1 cut(s) 1581
BbvI GCAGC 4 cut(s) 773, 961, 1267, 1462
BccI CCATC 5 cut(s) 277, 605, 758, 963, 1133
BcgI CGANNNNNNTGC 2 cut(s) 667, 701
BciT130I CCWGG 1 cut(s) 1529
BciVI GTATCC 1 cut(s) 1556
BclI TGATCA 1 cut(s) 1186
BcnI CCSGG 1 cut(s) 1678
BcoDI GTCTC 8 cut(s) 133, 194, 488, 1276, 1334, 1582, 1652, 1773
BcuI ACTAGT 1 cut(s) 1382
BfmI CTRYAG 2 cut(s) 190, 1774
BfuI GTATCC 1 cut(s) 1556
BglII AGATCT 1 cut(s) 883
BisI GCNGC 5 cut(s) 84, 787, 950, 1256, 1476
BlnI CCTAGG 2 cut(s) 1079, 1448
BlpI GCTNAGC 1 cut(s) 722
BlsI GCNGC 5 cut(s) 85, 788, 951, 1257, 1477
Bme1390I CCNGG 2 cut(s) 1529, 1678
BmgBI CACGTC 1 cut(s) 143
BmgT120I GGNCC 1 cut(s) 1561
BmiI GGNNCC 2 cut(s) 559, 1107
BmrFI CCNGG 2 cut(s) 1529, 1678
BmsI GCATC 6 cut(s) 256, 809, 847, 867, 1386, 1422
BpiI GAAGAC 1 cut(s) 1581
BpmI CTGGAG 1 cut(s) 1550
Bpu1102I GCTNAGC 1 cut(s) 722
Bpu14I TTCGAA 2 cut(s) 78, 1747
BpuEI CTTGAG 1 cut(s) 908
BpuMI CCSGG 1 cut(s) 1678
BsaHI GRCGYC 1 cut(s) 1586
BsaI GGTCTC 2 cut(s) 488, 1582
BsaJI CCNNGG 4 cut(s) 1079, 1109, 1305, 1448
Bsc4I CCNNNNNNNGG 3 cut(s) 752, 1558, 1569
Bse118I RCCGGY 1 cut(s) 374
Bse1I ACTGG 1 cut(s) 665
Bse3DI GCAATG 2 cut(s) 1157, 1485
BseBI CCWGG 1 cut(s) 1529
BseDI CCNNGG 4 cut(s) 1079, 1109, 1305, 1448
BseGI GGATG 9 cut(s) 180, 247, 706, 824, 838, 955, 1027, 1189, 1401
BseLI CCNNNNNNNGG 3 cut(s) 752, 1558, 1569
BseMI GCAATG 2 cut(s) 1157, 1485
BseMII CTCAG 4 cut(s) 195, 1170, 1514, 1595
BseNI ACTGG 1 cut(s) 665
BseRI GAGGAG 1 cut(s) 731
BseXI GCAGC 4 cut(s) 773, 961, 1267, 1462
Bsh1236I CGCG 1 cut(s) 1068
Bsh1285I CGRYCG 1 cut(s) 1009
BshFI GGCC 4 cut(s) 86, 630, 1443, 1562
BshNI GGYRCC 1 cut(s) 557
BsiEI CGRYCG 1 cut(s) 1009
BsiSI CCGG 2 cut(s) 375, 1677
BslI CCNNNNNNNGG 3 cut(s) 752, 1558, 1569
BsmAI GTCTC 8 cut(s) 133, 194, 488, 1276, 1334, 1582, 1652, 1773
BsmBI CGTCTC 2 cut(s) 133, 1276
BsmI GAATGC 1 cut(s) 19
BsnI GGCC 4 cut(s) 86, 630, 1443, 1562
Bso31I GGTCTC 2 cut(s) 488, 1582
Bsp119I TTCGAA 2 cut(s) 78, 1747
Bsp1286I GDGCHC 1 cut(s) 48
Bsp143I GATC 4 cut(s) 328, 619, 883, 1186
Bsp1720I GCTNAGC 1 cut(s) 722
Bsp19I CCATGG 1 cut(s) 1109
BspACI CCGC 6 cut(s) 83, 287, 808, 1491, 1549, 1711
BspANI GGCC 4 cut(s) 86, 630, 1443, 1562
BspCNI CTCAG 4 cut(s) 196, 1169, 1515, 1594
BspFNI CGCG 1 cut(s) 1068
BspHI TCATGA 1 cut(s) 1060
BspLI GGNNCC 2 cut(s) 559, 1107
BspPI GGATC 2 cut(s) 336, 627
BspT104I TTCGAA 2 cut(s) 78, 1747
BspT107I GGYRCC 1 cut(s) 557
BspTNI GGTCTC 2 cut(s) 488, 1582
BsrDI GCAATG 2 cut(s) 1157, 1485
BsrFI RCCGGY 1 cut(s) 374
BsrI ACTGG 1 cut(s) 665
BssAI RCCGGY 1 cut(s) 374
BssECI CCNNGG 4 cut(s) 1079, 1109, 1305, 1448
BssMI GATC 4 cut(s) 328, 619, 883, 1186
BssNI GRCGYC 1 cut(s) 1586
BssT1I CCWWGG 3 cut(s) 1079, 1109, 1448
Bst2UI CCWGG 1 cut(s) 1529
Bst4CI ACNGT 8 cut(s) 466, 482, 562, 914, 965, 1007, 1412, 1775
Bst6I CTCTTC 1 cut(s) 1614
BstACI GRCGYC 1 cut(s) 1586
BstBI TTCGAA 2 cut(s) 78, 1747
BstC8I GCNNGC 2 cut(s) 376, 784
BstDEI CTNAG 6 cut(s) 204, 722, 1156, 1391, 1523, 1581
BstDSI CCRYGG 1 cut(s) 1109
BstF5I GGATG 9 cut(s) 180, 247, 706, 824, 838, 955, 1027, 1189, 1401
BstFNI CGCG 1 cut(s) 1068
BstHHI GCGC 2 cut(s) 949, 1068
BstKTI GATC 4 cut(s) 331, 622, 886, 1189
BstMAI GTCTC 8 cut(s) 133, 194, 488, 1276, 1334, 1582, 1652, 1773
BstMBI GATC 4 cut(s) 328, 619, 883, 1186
BstMCI CGRYCG 1 cut(s) 1009
BstMWI GCNNNNNNNGC 2 cut(s) 502, 644
BstNI CCWGG 1 cut(s) 1529
BstSCI CCNGG 2 cut(s) 1527, 1676
BstSFI CTRYAG 2 cut(s) 190, 1774
BstUI CGCG 1 cut(s) 1068
BstV1I GCAGC 4 cut(s) 773, 961, 1267, 1462
BstV2I GAAGAC 1 cut(s) 1581
BstX2I RGATCY 2 cut(s) 328, 883
BstYI RGATCY 2 cut(s) 328, 883
BsuI GTATCC 1 cut(s) 1556
BsuRI GGCC 4 cut(s) 86, 630, 1443, 1562
BtgI CCRYGG 1 cut(s) 1109
BtrI CACGTC 1 cut(s) 143
BtsCI GGATG 9 cut(s) 180, 247, 706, 824, 838, 955, 1027, 1189, 1401
BtsI GCAGTG 2 cut(s) 393, 1713
BtsIMutI CAGTG 2 cut(s) 393, 1713
Cac8I GCNNGC 2 cut(s) 376, 784
CaiI CAGNNNCTG 1 cut(s) 1435
CciI TCATGA 1 cut(s) 1060
CfoI GCGC 2 cut(s) 949, 1068
Cfr10I RCCGGY 1 cut(s) 374
Cfr13I GGNCC 1 cut(s) 1561
CseI GACGC 1 cut(s) 397
Csp6I GTAC 3 cut(s) 558, 1217, 1811
CviAII CATG 7 cut(s) 98, 118, 750, 1061, 1110, 1494, 1718
CviQI GTAC 3 cut(s) 558, 1217, 1811
DdeI CTNAG 6 cut(s) 204, 722, 1156, 1391, 1523, 1581
DpnI GATC 4 cut(s) 330, 621, 885, 1188
DpnII GATC 4 cut(s) 328, 619, 883, 1186
DraI TTTAAA 1 cut(s) 1645
DraIII CACNNNGTG 1 cut(s) 752
Eam1104I CTCTTC 1 cut(s) 1614
EarI CTCTTC 1 cut(s) 1614
EciI GGCGGA 1 cut(s) 1538
Eco130I CCWWGG 3 cut(s) 1079, 1109, 1448
Eco24I GRGCYC 1 cut(s) 48
Eco31I GGTCTC 2 cut(s) 488, 1582
EcoRI GAATTC 1 cut(s) 1796
EcoRII CCWGG 1 cut(s) 1527
EcoT14I CCWWGG 3 cut(s) 1079, 1109, 1448
EcoT22I ATGCAT 1 cut(s) 824
EcoT38I GRGCYC 1 cut(s) 48
ErhI CCWWGG 3 cut(s) 1079, 1109, 1448
Esp3I CGTCTC 2 cut(s) 133, 1276
FaeI CATG 7 cut(s) 101, 121, 753, 1064, 1113, 1497, 1721
FatI CATG 7 cut(s) 97, 117, 749, 1060, 1109, 1493, 1717
FauI CCCGC 1 cut(s) 280
FauNDI CATATG 2 cut(s) 362, 1566
FbaI TGATCA 1 cut(s) 1186
Fnu4HI GCNGC 5 cut(s) 84, 787, 950, 1256, 1476
FokI GGATG 9 cut(s) 187, 234, 713, 825, 831, 942, 1014, 1196, 1408
FriOI GRGCYC 1 cut(s) 48
Fsp4HI GCNGC 5 cut(s) 84, 787, 950, 1256, 1476
GlaI GCGC 2 cut(s) 948, 1067
GluI GCNGC 5 cut(s) 84, 787, 950, 1256, 1476
GsuI CTGGAG 1 cut(s) 1550
HaeIII GGCC 4 cut(s) 86, 630, 1443, 1562
HapII CCGG 2 cut(s) 375, 1677
HgaI GACGC 1 cut(s) 397
HhaI GCGC 2 cut(s) 949, 1068
Hin1I GRCGYC 1 cut(s) 1586
Hin1II CATG 7 cut(s) 101, 121, 753, 1064, 1113, 1497, 1721
Hin6I GCGC 2 cut(s) 947, 1066
HinP1I GCGC 2 cut(s) 947, 1066
HindIII AAGCTT 2 cut(s) 724, 1628
HinfI GANTC 7 cut(s) 681, 737, 768, 1169, 1519, 1686, 1732
HpaII CCGG 2 cut(s) 375, 1677
HphI GGTGA 1 cut(s) 1646
Hpy166II GTNNAC 1 cut(s) 746
Hpy8I GTNNAC 1 cut(s) 746
Hpy99I CGWCG 1 cut(s) 1073
HpyAV CCTTC 4 cut(s) 117, 497, 1371, 1454
HpyCH4III ACNGT 8 cut(s) 466, 482, 562, 914, 965, 1007, 1412, 1775
HpyCH4IV ACGT 3 cut(s) 142, 706, 1586
HpyF10VI GCNNNNNNNGC 2 cut(s) 502, 644
HpyF3I CTNAG 6 cut(s) 204, 722, 1156, 1391, 1523, 1581
HpySE526I ACGT 3 cut(s) 142, 706, 1586
Hsp92I GRCGYC 1 cut(s) 1586
Hsp92II CATG 7 cut(s) 101, 121, 753, 1064, 1113, 1497, 1721
HspAI GCGC 2 cut(s) 947, 1066
KpnI GGTACC 1 cut(s) 561
KroI GCCGGC 1 cut(s) 374
KroNI GCCGGC 1 cut(s) 376
Ksp22I TGATCA 1 cut(s) 1186
Kzo9I GATC 4 cut(s) 328, 619, 883, 1186
LmnI GCTCC 5 cut(s) 718, 827, 904, 1105, 1757
Lsp1109I GCAGC 4 cut(s) 773, 961, 1267, 1462
LweI GCATC 6 cut(s) 256, 809, 847, 867, 1386, 1422
MaeII ACGT 3 cut(s) 142, 706, 1586
MaeIII GTNAC 4 cut(s) 236, 688, 914, 1378
MalI GATC 4 cut(s) 330, 621, 885, 1188
MboI GATC 4 cut(s) 328, 619, 883, 1186
MboII GAAGA 8 cut(s) 292, 590, 863, 1202, 1244, 1581, 1631, 1636
MfeI CAATTG 1 cut(s) 1653
MflI RGATCY 2 cut(s) 328, 883
MhlI GDGCHC 1 cut(s) 48
MlyI GAGTC 2 cut(s) 1163, 1726
MmeI TCCRAC 2 cut(s) 439, 1124
Mph1103I ATGCAT 1 cut(s) 824
MroNI GCCGGC 1 cut(s) 374
MroXI GAANNNNTTC 1 cut(s) 466
MseI TTAA 5 cut(s) 582, 1292, 1466, 1506, 1644
MspI CCGG 2 cut(s) 375, 1677
MspR9I CCNGG 2 cut(s) 1529, 1678
MunI CAATTG 1 cut(s) 1653
Mva1269I GAATGC 1 cut(s) 19
MvaI CCWGG 1 cut(s) 1529
MvnI CGCG 1 cut(s) 1068
MwoI GCNNNNNNNGC 2 cut(s) 502, 644
NaeI GCCGGC 1 cut(s) 376
NciI CCSGG 1 cut(s) 1678
NcoI CCATGG 1 cut(s) 1109
NdeI CATATG 2 cut(s) 362, 1566
NdeII GATC 4 cut(s) 328, 619, 883, 1186
NgoMIV GCCGGC 1 cut(s) 374
NlaIII CATG 7 cut(s) 101, 121, 753, 1064, 1113, 1497, 1721
NlaIV GGNNCC 2 cut(s) 559, 1107
NmuCI GTSAC 1 cut(s) 914
NsiI ATGCAT 1 cut(s) 824
NspV TTCGAA 2 cut(s) 78, 1747
PagI TCATGA 1 cut(s) 1060
PcsI WCGNNNNNNNCGW 1 cut(s) 1326
PctI GAATGC 1 cut(s) 19
PdiI GCCGGC 1 cut(s) 376
PdmI GAANNNNTTC 1 cut(s) 466
PfeI GAWTC 5 cut(s) 681, 737, 768, 1519, 1686
PflFI GACNNNGTC 1 cut(s) 1609
PflMI CCANNNNNTGG 2 cut(s) 752, 1558
PkrI GCNGC 5 cut(s) 85, 788, 951, 1257, 1477
PleI GAGTC 2 cut(s) 1163, 1726
PpsI GAGTC 2 cut(s) 1163, 1726
Psp6I CCWGG 1 cut(s) 1527
PspGI CCWGG 1 cut(s) 1527
PspN4I GGNNCC 2 cut(s) 559, 1107
PspPI GGNCC 1 cut(s) 1561
PstNI CAGNNNCTG 1 cut(s) 1435
PsuI RGATCY 2 cut(s) 328, 883
PsyI GACNNNGTC 1 cut(s) 1609
RsaI GTAC 3 cut(s) 559, 1218, 1812
RsaNI GTAC 3 cut(s) 558, 1217, 1811
SaqAI TTAA 5 cut(s) 582, 1292, 1466, 1506, 1644
SatI GCNGC 5 cut(s) 84, 787, 950, 1256, 1476
Sau3AI GATC 4 cut(s) 328, 619, 883, 1186
Sau96I GGNCC 1 cut(s) 1561
SchI GAGTC 2 cut(s) 1163, 1726
ScrFI CCNGG 2 cut(s) 1529, 1678
SduI GDGCHC 1 cut(s) 48
SfaNI GCATC 6 cut(s) 256, 809, 847, 867, 1386, 1422
SfcI CTRYAG 2 cut(s) 190, 1774
SfuI TTCGAA 2 cut(s) 78, 1747
SmlI CTYRAG 1 cut(s) 887
SmoI CTYRAG 1 cut(s) 887
SpeI ACTAGT 1 cut(s) 1382
SsiI CCGC 6 cut(s) 83, 287, 808, 1491, 1549, 1711
SspI AATATT 3 cut(s) 30, 799, 1124
StyD4I CCNGG 2 cut(s) 1527, 1676
StyI CCWWGG 3 cut(s) 1079, 1109, 1448
TaaI ACNGT 8 cut(s) 466, 482, 562, 914, 965, 1007, 1412, 1775
TaiI ACGT 3 cut(s) 145, 709, 1589
TaqI TCGA 4 cut(s) 58, 78, 1604, 1747
TaqII GACCGA 1 cut(s) 1310
TatI WGTACW 2 cut(s) 1216, 1810
TauI GCSGC 1 cut(s) 86
TfiI GAWTC 5 cut(s) 681, 737, 768, 1519, 1686
Tru1I TTAA 5 cut(s) 582, 1292, 1466, 1506, 1644
Tru9I TTAA 5 cut(s) 582, 1292, 1466, 1506, 1644
TscAI CASTG 2 cut(s) 400, 1713
TseFI GTSAC 1 cut(s) 914
TseI GCWGC 4 cut(s) 786, 949, 1255, 1475
Tsp45I GTSAC 1 cut(s) 914
TspDTI ATGAA 7 cut(s) 114, 191, 479, 717, 1259, 1632, 1805
TspGWI ACGGA 2 cut(s) 1086, 1300
TspRI CASTG 2 cut(s) 400, 1713
Tth111I GACNNNGTC 1 cut(s) 1609
Van91I CCANNNNNTGG 2 cut(s) 752, 1558
XapI RAATTY 5 cut(s) 416, 1247, 1538, 1637, 1796
XbaI TCTAGA 1 cut(s) 331
XmaJI CCTAGG 2 cut(s) 1079, 1448
XmnI GAANNNNTTC 1 cut(s) 466
ZraI GACGTC 1 cut(s) 1587
Zsp2I ATGCAT 1 cut(s) 824
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.