pycom10g07140

N-acetyl-gamma-glutamyl-phosphate reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0007780
Physical Location & Seq
Forward (+)
8350106 .. 8352317
2212 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 660 bp
ATGAACTCTGCGACTTTCAGCTCCACTTTTCTCCGAACCGGCTGCCTCCGCAAGGACTTGCAGGACGGGGTGAACAATTTGAAGGCCGATACGCGCAATGCAGGGAAGGTGTTTGTTAAATGTTATGTGAATACGAAAACCCAGAAAGCAGAAAAGGAAGTTCGGCTTGGTGTTCTTGGAGCCAGTGGTTACACTGGTTCTGAGATTGTTCGGCTTTTGGCAAACCATCCTCACTTTGGCATTACCTTGATGACGGCTGATAGAAAAGCTGGACAGTCAATAGGGTCGGTGTTTCCGCATTTAGTTTCCCAAGATCTACCAAAAATGGTTGCTATAAAGGATGCTGATTTTTCTGATGTGGATGCTGTTTTTTGTTGTTTGCCACATGGAACCACACAGGAAATTATCGAAGGCCTTCCCAAGAGTTTAAAGATTGTTGATCTGTCTGCTGACTTCCGTCTACGAGACATATCTGAGTATGAAGAATGGTATGGTCAGGCACATAGCGCACCAGAGTTGCAGAAAGAAGTTGTATATGGTTTGACAGAGATTTTGAGGGAGGAGATAAAAGGTGCGCGCGTAGTTGCGAACCCTGGTTGTTATCCAACTTCTGTTCAACTTCCACTTGTTCCATTGATCGATCAAGGCCAATCTCATTGA

Protein Analysis

220

Amino Acids

24.02

Weight (kDa)

6.3

Isoelectric Point (pI)

32.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Semialdhyde_dh PF01118 56 - 191 6.9e-29 Semialdehyde dehydrogenase, NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 460
AccII CGCG 3 cut(s) 94, 577, 579
AciI CCGC 2 cut(s) 49, 296
AfiI CCNNNNNNNGG 2 cut(s) 52, 236
AgsI TTSAA 2 cut(s) 82, 617
AjnI CCWGG 1 cut(s) 592
AjuI GAANNNNNNNTTGG 2 cut(s) 150, 182
AluBI AGCT 2 cut(s) 21, 269
AluI AGCT 2 cut(s) 21, 269
Alw26I GTCTC 1 cut(s) 459
AoxI GGCC 3 cut(s) 84, 412, 646
ApeKI GCWGC 1 cut(s) 42
ArsI GACNNNNNNTTYG 2 cut(s) 535, 567
Asp700I GAANNNNTTC 1 cut(s) 414
AspLEI GCGC 4 cut(s) 96, 509, 577, 579
AsuHPI GGTGA 1 cut(s) 82
BbvI GCAGC 1 cut(s) 29
BccI CCATC 1 cut(s) 234
BceAI ACGGC 1 cut(s) 270
BcgI CGANNNNNNTGC 2 cut(s) 24, 58
BciT130I CCWGG 1 cut(s) 594
BcoDI GTCTC 1 cut(s) 459
BglII AGATCT 1 cut(s) 313
BisI GCNGC 1 cut(s) 43
BlsI GCNGC 1 cut(s) 44
Bme1390I CCNGG 1 cut(s) 594
BmiI GGNNCC 2 cut(s) 181, 391
BmrFI CCNGG 1 cut(s) 594
BmsI GCATC 2 cut(s) 331, 352
BoxI GACNNNNGTC 1 cut(s) 456
Bsa29I ATCGAT 1 cut(s) 639
BsaJI CCNNGG 1 cut(s) 592
Bsc4I CCNNNNNNNGG 2 cut(s) 52, 236
Bse118I RCCGGY 1 cut(s) 38
Bse1I ACTGG 2 cut(s) 183, 199
Bse3DI GCAATG 1 cut(s) 103
BseBI CCWGG 1 cut(s) 594
BseCI ATCGAT 1 cut(s) 639
BseDI CCNNGG 1 cut(s) 592
BseGI GGATG 3 cut(s) 226, 346, 367
BseLI CCNNNNNNNGG 2 cut(s) 52, 236
BseMI GCAATG 1 cut(s) 103
BseMII CTCAG 2 cut(s) 192, 465
BseNI ACTGG 2 cut(s) 183, 199
BsePI GCGCGC 1 cut(s) 575
BseRI GAGGAG 1 cut(s) 575
BseXI GCAGC 1 cut(s) 29
Bsh1236I CGCG 3 cut(s) 94, 577, 579
BshFI GGCC 3 cut(s) 86, 414, 648
BshVI ATCGAT 1 cut(s) 639
BsiSI CCGG 1 cut(s) 39
BslI CCNNNNNNNGG 2 cut(s) 52, 236
BsmAI GTCTC 1 cut(s) 459
BsnI GGCC 3 cut(s) 86, 414, 648
Bsp143I GATC 4 cut(s) 313, 439, 636, 640
BspACI CCGC 2 cut(s) 49, 296
BspANI GGCC 3 cut(s) 86, 414, 648
BspCNI CTCAG 2 cut(s) 193, 466
BspDI ATCGAT 1 cut(s) 639
BspFNI CGCG 3 cut(s) 94, 577, 579
BspLI GGNNCC 2 cut(s) 181, 391
BsrDI GCAATG 1 cut(s) 103
BsrFI RCCGGY 1 cut(s) 38
BsrI ACTGG 2 cut(s) 183, 199
BssAI RCCGGY 1 cut(s) 38
BssECI CCNNGG 1 cut(s) 592
BssHII GCGCGC 1 cut(s) 575
BssMI GATC 4 cut(s) 313, 439, 636, 640
Bst2UI CCWGG 1 cut(s) 594
Bst4CI ACNGT 1 cut(s) 276
BstC8I GCNNGC 1 cut(s) 577
BstDEI CTNAG 2 cut(s) 201, 474
BstENI CCTNNNNNAGG 1 cut(s) 50
BstF5I GGATG 3 cut(s) 226, 346, 367
BstFNI CGCG 3 cut(s) 94, 577, 579
BstHHI GCGC 4 cut(s) 96, 509, 577, 579
BstKTI GATC 4 cut(s) 316, 442, 639, 643
BstMAI GTCTC 1 cut(s) 459
BstMBI GATC 4 cut(s) 313, 439, 636, 640
BstMWI GCNNNNNNNGC 2 cut(s) 48, 506
BstNI CCWGG 1 cut(s) 594
BstPAI GACNNNNGTC 1 cut(s) 456
BstSCI CCNGG 1 cut(s) 592
BstUI CGCG 3 cut(s) 94, 577, 579
BstV1I GCAGC 1 cut(s) 29
BstX2I RGATCY 1 cut(s) 313
BstYI RGATCY 1 cut(s) 313
Bsu15I ATCGAT 1 cut(s) 639
BsuRI GGCC 3 cut(s) 86, 414, 648
BsuTUI ATCGAT 1 cut(s) 639
BtsCI GGATG 3 cut(s) 226, 346, 367
BtsIMutI CAGTG 2 cut(s) 190, 192
Cac8I GCNNGC 1 cut(s) 577
CfoI GCGC 4 cut(s) 96, 509, 577, 579
Cfr10I RCCGGY 1 cut(s) 38
ClaI ATCGAT 1 cut(s) 639
CviAII CATG 1 cut(s) 386
DdeI CTNAG 2 cut(s) 201, 474
DpnI GATC 4 cut(s) 315, 441, 638, 642
DpnII GATC 4 cut(s) 313, 439, 636, 640
DraI TTTAAA 1 cut(s) 429
Eco147I AGGCCT 1 cut(s) 414
EcoNI CCTNNNNNAGG 1 cut(s) 50
EcoRII CCWGG 1 cut(s) 592
FaeI CATG 1 cut(s) 389
FaiI YATR 9 cut(s) 126, 335, 387, 470, 480, 492, 504, 535, 537
FalI AAGNNNNNCTT 2 cut(s) 150, 182
FatI CATG 1 cut(s) 385
FblI GTMKAC 1 cut(s) 460
Fnu4HI GCNGC 1 cut(s) 43
FokI GGATG 3 cut(s) 213, 353, 374
Fsp4HI GCNGC 1 cut(s) 43
GlaI GCGC 4 cut(s) 95, 508, 576, 578
GluI GCNGC 1 cut(s) 43
HaeIII GGCC 3 cut(s) 86, 414, 648
HapII CCGG 1 cut(s) 39
HhaI GCGC 4 cut(s) 96, 509, 577, 579
Hin1II CATG 1 cut(s) 389
Hin6I GCGC 4 cut(s) 94, 507, 575, 577
HinP1I GCGC 4 cut(s) 94, 507, 575, 577
HpaII CCGG 1 cut(s) 39
HphI GGTGA 1 cut(s) 82
Hpy166II GTNNAC 2 cut(s) 73, 461
Hpy188I TCNGA 4 cut(s) 35, 202, 355, 475
Hpy8I GTNNAC 2 cut(s) 73, 461
HpyAV CCTTC 4 cut(s) 76, 100, 404, 425
HpyCH4III ACNGT 1 cut(s) 276
HpyCH4V TGCA 3 cut(s) 61, 101, 520
HpyF10VI GCNNNNNNNGC 2 cut(s) 48, 506
HpyF3I CTNAG 2 cut(s) 201, 474
Hsp92II CATG 1 cut(s) 389
HspAI GCGC 4 cut(s) 94, 507, 575, 577
Kzo9I GATC 4 cut(s) 313, 439, 636, 640
LmnI GCTCC 2 cut(s) 26, 179
Lsp1109I GCAGC 1 cut(s) 29
LweI GCATC 2 cut(s) 331, 352
MaeIII GTNAC 1 cut(s) 188
MalI GATC 4 cut(s) 315, 441, 638, 642
MboI GATC 4 cut(s) 313, 439, 636, 640
MboII GAAGA 1 cut(s) 494
MflI RGATCY 1 cut(s) 313
MluCI AATT 2 cut(s) 76, 402
MmeI TCCRAC 1 cut(s) 629
MnlI CCTC 4 cut(s) 56, 240, 549, 553
MroXI GAANNNNTTC 1 cut(s) 414
MseI TTAA 2 cut(s) 117, 428
MspI CCGG 1 cut(s) 39
MspR9I CCNGG 1 cut(s) 594
MvaI CCWGG 1 cut(s) 594
MvnI CGCG 3 cut(s) 94, 577, 579
MwoI GCNNNNNNNGC 2 cut(s) 48, 506
NdeII GATC 4 cut(s) 313, 439, 636, 640
NlaIII CATG 1 cut(s) 389
NlaIV GGNNCC 2 cut(s) 181, 391
PauI GCGCGC 1 cut(s) 575
PceI AGGCCT 1 cut(s) 414
PdmI GAANNNNTTC 1 cut(s) 414
PkrI GCNGC 1 cut(s) 44
PshAI GACNNNNGTC 1 cut(s) 456
Psp6I CCWGG 1 cut(s) 592
PspGI CCWGG 1 cut(s) 592
PspN4I GGNNCC 2 cut(s) 181, 391
PsuI RGATCY 1 cut(s) 313
PteI GCGCGC 1 cut(s) 575
SaqAI TTAA 2 cut(s) 117, 428
SatI GCNGC 1 cut(s) 43
Sau3AI GATC 4 cut(s) 313, 439, 636, 640
ScrFI CCNGG 1 cut(s) 594
SetI ASST 5 cut(s) 23, 111, 248, 271, 574
SfaNI GCATC 2 cut(s) 331, 352
Sse9I AATT 2 cut(s) 76, 402
SseBI AGGCCT 1 cut(s) 414
SsiI CCGC 2 cut(s) 49, 296
StuI AGGCCT 1 cut(s) 414
StyD4I CCNGG 1 cut(s) 592
TaaI ACNGT 1 cut(s) 276
TaqI TCGA 2 cut(s) 408, 639
TasI AATT 2 cut(s) 76, 402
Tru1I TTAA 2 cut(s) 117, 428
Tru9I TTAA 2 cut(s) 117, 428
TscAI CASTG 2 cut(s) 190, 199
TseI GCWGC 1 cut(s) 42
TspDTI ATGAA 2 cut(s) 17, 495
TspGWI ACGGA 1 cut(s) 446
TspRI CASTG 2 cut(s) 190, 199
XagI CCTNNNNNAGG 1 cut(s) 50
XcmI CCANNNNNNNNNTGG 1 cut(s) 233
XmiI GTMKAC 1 cut(s) 460
XmnI GAANNNNTTC 1 cut(s) 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.