pycom10g08800

Prolyl 4-hydroxylase alpha subunit homologues.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0010783
Physical Location & Seq
Forward (+)
11630428 .. 11645086
14659 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 825 bp
ATGAAAGTGAAAGCAAAGAACTCCAGAGAAAAGTTGGGCTCACCCGCCGTCTTCCTCCTCTGCTCCCTCTTCTTCTTCGCCGGCTTATTCATCTCCACCCTCCTCTCCCATGCCTCCGTCTCTCTCTTCCTCTCCGGCTCTCGACCCGTCTCCAGAACGCTGGAATCGGAAGACGACGAAGATCACGGTCCAATACTGCAGGGTGACACCGGAGACAGCTTTATCCCGTCGATTCCATTCCAGGTTTTGAGTTGGAGACCGCGCGCTCTGTATTTTCCGAGATTTGCAACTGCGGAGCAGTGCGAAGGGGTGATTGAGATGGCAAAGATGAAGCTCCGGCCGTCCACATTGGCATTGCGAAAGGGAGAGACTGCCGAGAGCACAAAGGGGACTCGAACAAGTTCAGGCACATTTATTAGCGCATCAGAAGACGATATTGGAGTCCTGGATATTATTGAGGAAAAGAAAGCCAGGGCAACAATGTTACCAAGAACCCATGGCGAGGCACTCAATGTTTTGCGGTATGAGATTGGCCAGAAGTACGATTCTCATTATGATGCATTCAATCCAGTGGAATATGGCCAACAGAAAAGGCAAAGATTTGCTTCCTTCTTGTTGTTTCTGTCTAATGTTGAAGAAGGTGGAGAAACCATGTTCCCTTTTGAGAATGGTGCAGAGATGGGTATGAGCTATGATTACAAGAAATGCATCGGTTTAAAAATTATGCCGAAGCAAGGGGATGGACTTTTATTTTATTCGGTGTTTCGAAATGGCACAATAGATCCGACATCTCTTCATGGAAGCTGCCCTGTAAGCAAAGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00330 ko01100 map00330 map01100
Pfam Domains
Protein Families
2OG-FeII_Oxy_3

Protein Analysis

275

Amino Acids

30.37

Weight (kDa)

6.54

Isoelectric Point (pI)

48.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy_3 PF13640 171 - 273 5.2e-10 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010783)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33910
fragaria_vesca FvH4_2g03890 FvH4_2g03890
malus_domestica MD05G1096100.v1.1 MD10G1102300.v1.1
prunus_persica Prupe.8G140500_v2.0.a1
pyrus_communis pycom05g09490 pycom10g08800
rosa_chinensis RchiOBHm_Chr6g0252911
rosa_laevigata RLG00000015049 RLG00000032764
rosa_multiflora Rmu_co8070424.1_g000001 Rmu_sc0000829.1_g000001
rosa_roxburghii Rroxscaffold_7G00212300
rosa_rugosa Rorug05G0540000
rosa_samantha Rh6AG055700 Rh6BG050000 Rh6CG048400 Rh6DG044000
rosa_wichuraiana Rw6G005040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 262, 264
AciI CCGC 4 cut(s) 45, 260, 293, 520
AclWI GGATC 1 cut(s) 776
AcoI YGGCCR 3 cut(s) 338, 532, 580
AfaI GTAC 1 cut(s) 542
AfiI CCNNNNNNNGG 2 cut(s) 502, 734
AgsI TTSAA 2 cut(s) 565, 635
AjnI CCWGG 3 cut(s) 240, 444, 470
AjuI GAANNNNNNNTTGG 2 cut(s) 420, 452
AluBI AGCT 4 cut(s) 219, 334, 690, 804
AluI AGCT 4 cut(s) 219, 334, 690, 804
Alw21I GWGCWC 1 cut(s) 383
Alw26I GTCTC 5 cut(s) 124, 154, 207, 250, 362
AlwI GGATC 1 cut(s) 776
AoxI GGCC 3 cut(s) 338, 532, 580
ApeKI GCWGC 1 cut(s) 804
Asp700I GAANNNNTTC 1 cut(s) 400
AspLEI GCGC 3 cut(s) 264, 266, 422
AspS9I GGNCC 1 cut(s) 188
AsuHPI GGTGA 3 cut(s) 33, 215, 322
AsuII TTCGAA 1 cut(s) 766
AvaII GGWCC 1 cut(s) 188
BalI TGGCCA 2 cut(s) 534, 582
BanII GRGCYC 1 cut(s) 41
BbsI GAAGAC 3 cut(s) 43, 177, 435
Bbv12I GWGCWC 1 cut(s) 383
BbvI GCAGC 1 cut(s) 791
BccI CCATC 3 cut(s) 313, 673, 734
BceAI ACGGC 2 cut(s) 32, 325
BciT130I CCWGG 3 cut(s) 242, 446, 472
BcoDI GTCTC 5 cut(s) 124, 154, 207, 250, 362
BfmI CTRYAG 1 cut(s) 197
BisI GCNGC 1 cut(s) 805
BlsI GCNGC 1 cut(s) 806
Bme1390I CCNGG 3 cut(s) 242, 446, 472
Bme18I GGWCC 1 cut(s) 188
BmgT120I GGNCC 1 cut(s) 188
BmrFI CCNGG 3 cut(s) 242, 446, 472
BmsI GCATC 3 cut(s) 431, 547, 717
BpiI GAAGAC 3 cut(s) 43, 177, 435
BpmI CTGGAG 2 cut(s) 7, 136
Bpu14I TTCGAA 1 cut(s) 766
BsaI GGTCTC 1 cut(s) 250
BsaJI CCNNGG 2 cut(s) 471, 496
BsaWI WCCGGW 1 cut(s) 209
Bsc4I CCNNNNNNNGG 2 cut(s) 502, 734
Bse118I RCCGGY 1 cut(s) 80
Bse1I ACTGG 1 cut(s) 569
Bse3DI GCAATG 1 cut(s) 353
BseBI CCWGG 3 cut(s) 242, 446, 472
BseDI CCNNGG 2 cut(s) 471, 496
BseGI GGATG 1 cut(s) 745
BseLI CCNNNNNNNGG 2 cut(s) 502, 734
BseMI GCAATG 1 cut(s) 353
BseNI ACTGG 1 cut(s) 569
BsePI GCGCGC 1 cut(s) 262
BseRI GAGGAG 2 cut(s) 47, 92
BseX3I CGGCCG 1 cut(s) 338
BseXI GCAGC 1 cut(s) 791
BsgI GTGCAG 1 cut(s) 693
Bsh1236I CGCG 2 cut(s) 262, 264
Bsh1285I CGRYCG 1 cut(s) 341
BshFI GGCC 3 cut(s) 340, 534, 582
BsiEI CGRYCG 1 cut(s) 341
BsiHKAI GWGCWC 1 cut(s) 383
BsiSI CCGG 4 cut(s) 81, 135, 210, 337
BslFI GGGAC 1 cut(s) 403
BslI CCNNNNNNNGG 2 cut(s) 502, 734
BsmAI GTCTC 5 cut(s) 124, 154, 207, 250, 362
BsmBI CGTCTC 2 cut(s) 124, 154
BsmFI GGGAC 1 cut(s) 403
BsmI GAATGC 1 cut(s) 560
BsnI GGCC 3 cut(s) 340, 534, 582
Bso31I GGTCTC 1 cut(s) 250
Bsp119I TTCGAA 1 cut(s) 766
Bsp1286I GDGCHC 2 cut(s) 41, 383
Bsp143I GATC 2 cut(s) 181, 781
Bsp19I CCATGG 1 cut(s) 496
BspACI CCGC 4 cut(s) 45, 260, 293, 520
BspANI GGCC 3 cut(s) 340, 534, 582
BspFNI CGCG 2 cut(s) 262, 264
BspMAI CTGCAG 1 cut(s) 201
BspPI GGATC 1 cut(s) 776
BspT104I TTCGAA 1 cut(s) 766
BspTNI GGTCTC 1 cut(s) 250
BsrDI GCAATG 1 cut(s) 353
BsrFI RCCGGY 1 cut(s) 80
BsrI ACTGG 1 cut(s) 569
BssAI RCCGGY 1 cut(s) 80
BssECI CCNNGG 2 cut(s) 471, 496
BssHII GCGCGC 1 cut(s) 262
BssMI GATC 2 cut(s) 181, 781
BssT1I CCWWGG 1 cut(s) 496
Bst2UI CCWGG 3 cut(s) 242, 446, 472
Bst4CI ACNGT 1 cut(s) 188
Bst6I CTCTTC 3 cut(s) 74, 131, 798
BstBI TTCGAA 1 cut(s) 766
BstC8I GCNNGC 2 cut(s) 82, 264
BstDSI CCRYGG 1 cut(s) 496
BstF5I GGATG 1 cut(s) 745
BstFNI CGCG 2 cut(s) 262, 264
BstHHI GCGC 3 cut(s) 264, 266, 422
BstKTI GATC 2 cut(s) 184, 784
BstMAI GTCTC 5 cut(s) 124, 154, 207, 250, 362
BstMBI GATC 2 cut(s) 181, 781
BstMCI CGRYCG 1 cut(s) 341
BstMWI GCNNNNNNNGC 1 cut(s) 813
BstNI CCWGG 3 cut(s) 242, 446, 472
BstSCI CCNGG 3 cut(s) 240, 444, 470
BstSFI CTRYAG 1 cut(s) 197
BstUI CGCG 2 cut(s) 262, 264
BstV1I GCAGC 1 cut(s) 791
BstV2I GAAGAC 3 cut(s) 43, 177, 435
BstX2I RGATCY 1 cut(s) 781
BstXI CCANNNNNNTGG 1 cut(s) 160
BstYI RGATCY 1 cut(s) 781
BstZI CGGCCG 1 cut(s) 338
BsuRI GGCC 3 cut(s) 340, 534, 582
BtgI CCRYGG 1 cut(s) 496
BtsCI GGATG 1 cut(s) 745
BtsI GCAGTG 1 cut(s) 305
BtsIMutI CAGTG 2 cut(s) 305, 576
Cac8I GCNNGC 2 cut(s) 82, 264
CfoI GCGC 3 cut(s) 264, 266, 422
Cfr10I RCCGGY 1 cut(s) 80
Cfr13I GGNCC 1 cut(s) 188
Csp6I GTAC 1 cut(s) 541
CviAII CATG 4 cut(s) 110, 497, 652, 797
CviQI GTAC 1 cut(s) 541
DpnI GATC 2 cut(s) 183, 783
DpnII GATC 2 cut(s) 181, 781
DraI TTTAAA 1 cut(s) 717
EaeI YGGCCR 3 cut(s) 338, 532, 580
EagI CGGCCG 1 cut(s) 338
Eam1104I CTCTTC 3 cut(s) 74, 131, 798
EarI CTCTTC 3 cut(s) 74, 131, 798
EclXI CGGCCG 1 cut(s) 338
Eco130I CCWWGG 1 cut(s) 496
Eco24I GRGCYC 1 cut(s) 41
Eco31I GGTCTC 1 cut(s) 250
Eco47I GGWCC 1 cut(s) 188
Eco52I CGGCCG 1 cut(s) 338
EcoRII CCWGG 3 cut(s) 240, 444, 470
EcoT14I CCWWGG 1 cut(s) 496
EcoT22I ATGCAT 2 cut(s) 562, 710
EcoT38I GRGCYC 1 cut(s) 41
ErhI CCWWGG 1 cut(s) 496
Esp3I CGTCTC 2 cut(s) 124, 154
FaeI CATG 4 cut(s) 113, 500, 655, 800
FalI AAGNNNNNCTT 2 cut(s) 589, 621
FaqI GGGAC 1 cut(s) 403
FatI CATG 4 cut(s) 109, 496, 651, 796
FauI CCCGC 1 cut(s) 52
Fnu4HI GCNGC 1 cut(s) 805
FokI GGATG 1 cut(s) 752
FriOI GRGCYC 1 cut(s) 41
Fsp4HI GCNGC 1 cut(s) 805
GlaI GCGC 3 cut(s) 263, 265, 421
GluI GCNGC 1 cut(s) 805
GsuI CTGGAG 2 cut(s) 7, 136
HaeIII GGCC 3 cut(s) 340, 534, 582
HapII CCGG 4 cut(s) 81, 135, 210, 337
HhaI GCGC 3 cut(s) 264, 266, 422
Hin1II CATG 4 cut(s) 113, 500, 655, 800
Hin6I GCGC 3 cut(s) 262, 264, 420
HinP1I GCGC 3 cut(s) 262, 264, 420
HinfI GANTC 5 cut(s) 164, 232, 391, 441, 545
HpaII CCGG 4 cut(s) 81, 135, 210, 337
HphI GGTGA 3 cut(s) 33, 215, 322
Hpy166II GTNNAC 1 cut(s) 345
Hpy188I TCNGA 4 cut(s) 169, 279, 427, 786
Hpy188III TCNNGA 3 cut(s) 24, 141, 153
Hpy8I GTNNAC 1 cut(s) 345
Hpy99I CGWCG 2 cut(s) 179, 232
HpyAV CCTTC 3 cut(s) 299, 619, 632
HpyCH4III ACNGT 1 cut(s) 188
HpyCH4V TGCA 5 cut(s) 199, 287, 560, 674, 708
HpyF10VI GCNNNNNNNGC 1 cut(s) 813
Hsp92II CATG 4 cut(s) 113, 500, 655, 800
HspAI GCGC 3 cut(s) 262, 264, 420
KroI GCCGGC 1 cut(s) 80
KroNI GCCGGC 1 cut(s) 82
Kzo9I GATC 2 cut(s) 181, 781
LmnI GCTCC 3 cut(s) 68, 295, 339
Lsp1109I GCAGC 1 cut(s) 791
LweI GCATC 3 cut(s) 431, 547, 717
MaeIII GTNAC 2 cut(s) 203, 483
MalI GATC 2 cut(s) 183, 783
MboI GATC 2 cut(s) 181, 781
MflI RGATCY 1 cut(s) 781
MhlI GDGCHC 2 cut(s) 41, 383
MlsI TGGCCA 2 cut(s) 534, 582
MluCI AATT 1 cut(s) 720
MluNI TGGCCA 2 cut(s) 534, 582
MlyI GAGTC 2 cut(s) 385, 450
MmeI TCCRAC 2 cut(s) 233, 809
MnlI CCTC 9 cut(s) 65, 68, 77, 110, 113, 124, 140, 451, 496
Mox20I TGGCCA 2 cut(s) 534, 582
Mph1103I ATGCAT 2 cut(s) 562, 710
MroNI GCCGGC 1 cut(s) 80
MroXI GAANNNNTTC 1 cut(s) 400
MscI TGGCCA 2 cut(s) 534, 582
MseI TTAA 1 cut(s) 716
MslI CAYNNNNRTG 1 cut(s) 555
Msp20I TGGCCA 2 cut(s) 534, 582
MspI CCGG 4 cut(s) 81, 135, 210, 337
MspR9I CCNGG 3 cut(s) 242, 446, 472
Mva1269I GAATGC 1 cut(s) 560
MvaI CCWGG 3 cut(s) 242, 446, 472
MvnI CGCG 2 cut(s) 262, 264
MwoI GCNNNNNNNGC 1 cut(s) 813
NaeI GCCGGC 1 cut(s) 82
NcoI CCATGG 1 cut(s) 496
NdeII GATC 2 cut(s) 181, 781
NgoMIV GCCGGC 1 cut(s) 80
NlaIII CATG 4 cut(s) 113, 500, 655, 800
NmeAIII GCCGAG 1 cut(s) 400
NmuCI GTSAC 1 cut(s) 203
NsiI ATGCAT 2 cut(s) 562, 710
NspV TTCGAA 1 cut(s) 766
PauI GCGCGC 1 cut(s) 262
PctI GAATGC 1 cut(s) 560
PdiI GCCGGC 1 cut(s) 82
PdmI GAANNNNTTC 1 cut(s) 400
PfeI GAWTC 3 cut(s) 164, 232, 545
PfoI TCCNGGA 1 cut(s) 444
PkrI GCNGC 1 cut(s) 806
PleI GAGTC 2 cut(s) 385, 449
PpsI GAGTC 2 cut(s) 385, 449
Psp6I CCWGG 3 cut(s) 240, 444, 470
PspGI CCWGG 3 cut(s) 240, 444, 470
PspPI GGNCC 1 cut(s) 188
PstI CTGCAG 1 cut(s) 201
PsuI RGATCY 1 cut(s) 781
PteI GCGCGC 1 cut(s) 262
RsaI GTAC 1 cut(s) 542
RsaNI GTAC 1 cut(s) 541
RseI CAYNNNNRTG 1 cut(s) 555
SaqAI TTAA 1 cut(s) 716
SatI GCNGC 1 cut(s) 805
Sau3AI GATC 2 cut(s) 181, 781
Sau96I GGNCC 1 cut(s) 188
SchI GAGTC 2 cut(s) 385, 450
ScrFI CCNGG 3 cut(s) 242, 446, 472
SduI GDGCHC 2 cut(s) 41, 383
SetI ASST 6 cut(s) 221, 246, 336, 643, 692, 806
SfaNI GCATC 3 cut(s) 431, 547, 717
SfcI CTRYAG 1 cut(s) 197
SfuI TTCGAA 1 cut(s) 766
SinI GGWCC 1 cut(s) 188
SmiMI CAYNNNNRTG 1 cut(s) 555
Sse9I AATT 1 cut(s) 720
SsiI CCGC 4 cut(s) 45, 260, 293, 520
StyD4I CCNGG 3 cut(s) 240, 444, 470
StyI CCWWGG 1 cut(s) 496
TaaI ACNGT 1 cut(s) 188
TaqI TCGA 4 cut(s) 142, 230, 394, 766
TasI AATT 1 cut(s) 720
TfiI GAWTC 3 cut(s) 164, 232, 545
Tru1I TTAA 1 cut(s) 716
Tru9I TTAA 1 cut(s) 716
TscAI CASTG 2 cut(s) 305, 576
TseFI GTSAC 1 cut(s) 203
TseI GCWGC 1 cut(s) 804
Tsp45I GTSAC 1 cut(s) 203
TspDTI ATGAA 4 cut(s) 17, 79, 344, 785
TspGWI ACGGA 1 cut(s) 106
TspRI CASTG 2 cut(s) 305, 576
VpaK11BI GGWCC 1 cut(s) 188
XcmI CCANNNNNNNNNTGG 1 cut(s) 31
XmnI GAANNNNTTC 1 cut(s) 400
Zsp2I ATGCAT 2 cut(s) 562, 710
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.