Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0006785

Physical Location & Seq

Genomic Coordinates Reverse (-)

12512612 .. 12514928

Gene Structure 2317 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 888 bp

ATGCAAACCCTAACCCCACTACGACCTCGTTTTGCAAGGCTCTTGAGCTCTAATCCTCTATTGTCGCTCTCTCTCAGACCCTCGACCACTCGAGCATTCTCCTCTTCTTCCTCGTCTTCAGATTTCTCCAACCAGTCCCGCGGCGGCCTCCCTCGCTTCTTCTCCGAGCTCATCCCTGCTTCCAAGGGAGGCGTAGTCCGCGTACAAGGCGACGAGTTCTGGCACATGACTAAAGTTTTGAGATTGCGCGCCAATGATAGGGTAGAGCTTTTCAATGGGAAAGGAAGTTTGATACAAGGGTTAATACAGAGCGTCGACCGCTCTGGGCTTGATTTTGTGGCATTGGAAGATCCAAAGTTGGTTCTTCCACAGAACACACAGTGGCATGTGTTTGCGGCTTTTGGTACTTTGAAGGGCGGTCGAGCTGACTGGCTTGTTGAGAAATGCACGGAGCTGGGGGCTAGTAGTTTAACCCCTCTGCTGACAGAGCGGTCTCCTACTATCTCAGAAAATCGGGTGGACAGGTTGCAGCGTGTCAACATGGCGGCAGCTAAACAATGCCAACGGCTACATGAAATGATTATGAATCCTCCAGTGAAAGTTGATGGTCTTTTACCTCTTGTTGCACAGTCAAAGCTAGCTATTTTGGCAACTGCAGAAGCTACTCCTCTTGTTAGTGCATTAACTTCATCAGGATGGGAGTCCAGTGGGCTGATTGTTGTTGGACCAGAAGGTGACTTCACTGAGAAAGAGGTGAGTGAATTGATGGAAGCTGGTGCTATCTCAGTTGGTCTTGGCCCACATCGACTACGGGTTGAAACTGCAACCGTGGCTCTTCTGGCAACTCTCATGTTATGGTCTGATTCTCAAAAAGCATGTCATTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000154 GO:0001510 GO:0003674 GO:0003824 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008173 GO:0008649 GO:0008757 GO:0009451 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0016436 GO:0016740 GO:0016741 GO:0022613 GO:0031167 GO:0032259 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0043170 GO:0043412 GO:0043414 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0046483 GO:0070042 GO:0070475 GO:0071704 GO:0071840 GO:0090304 GO:0140098 GO:0140102 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Methyltrans_RNA

Protein Analysis

296

Amino Acids

32.0

Weight (kDa)

8.86

Isoelectric Point (pI)

43.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PUA_4	PF20260	69 - 113	1.7e-11	RNA methyltransferase PUA domain
Methyltrans_RNA	PF04452	128 - 283	1.9e-36	RNA methyltransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006785)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G50000 AT1G50000 AT1G50000 AT1G50000 AT1G50000
fragaria_vesca	FvH4_2g03440 FvH4_2g03440 FvH4_2g03440 FvH4_2g03440
malus_domestica	MD10G1109100.v1.1
prunus_persica	Prupe.8G149200_v2.0.a1 Prupe.8G149200_v2.0.a1 Prupe.8G149200_v2.0.a1
pyrus_communis	pycom10g09440
rosa_chinensis	RchiOBHm_Chr6g0248601
rosa_laevigata	RLG00000015140
rosa_multiflora	Rmu_sc0002553.1_g000038
rosa_roxburghii	Rroxscaffold_7G00213180
rosa_rugosa	Rorug05G0532400 Rorug05G0532400
rosa_samantha	Rh6AG049300 Rh6BG045100 Rh6CG041300 Rh6DG037600
rosa_wichuraiana	Rw6G004260 Rw6G004360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	490
AccBSI	CCGCTC	2 cut(s)	321, 490
AccI	GTMKAC	1 cut(s)	315
AccII	CGCG	3 cut(s)	141, 201, 249
AciI	CCGC	9 cut(s)	139, 141, 144, 199, 319, 395, 417, 490, 545
AclWI	GGATC	1 cut(s)	344
AcuI	CTGAAG	1 cut(s)	102
AdeI	CACNNNGTG	1 cut(s)	381
AfaI	GTAC	2 cut(s)	204, 406
AfiI	CCNNNNNNNGG	1 cut(s)	258
AgsI	TTSAA	3 cut(s)	274, 412, 818
Alw21I	GWGCWC	2 cut(s)	50, 171
Alw26I	GTCTC	1 cut(s)	498
AlwI	GGATC	1 cut(s)	344
Ama87I	CYCGRG	1 cut(s)	90
AoxI	GGCC	2 cut(s)	145, 796
ApeKI	GCWGC	2 cut(s)	529, 548
ArsI	GACNNNNNNTTYG	3 cut(s)	220, 252, 862
AspLEI	GCGC	2 cut(s)	249, 251
AspS9I	GGNCC	2 cut(s)	725, 797
AsuHPI	GGTGA	2 cut(s)	746, 766
AsuNHI	GCTAGC	1 cut(s)	637
AvaI	CYCGRG	1 cut(s)	90
AvaII	GGWCC	1 cut(s)	725
BaeI	ACNNNNGTAYC	2 cut(s)	284, 317
BanII	GRGCYC	2 cut(s)	50, 171
BbsI	GAAGAC	1 cut(s)	108
Bbv12I	GWGCWC	2 cut(s)	50, 171
BbvI	GCAGC	2 cut(s)	541, 560
BccI	CCATC	3 cut(s)	599, 690, 760
BceAI	ACGGC	1 cut(s)	581
BcoDI	GTCTC	1 cut(s)	498
BfaI	CTAG	2 cut(s)	462, 638
BfmI	CTRYAG	1 cut(s)	654
BisI	GCNGC	6 cut(s)	142, 145, 396, 530, 546, 549
BlsI	GCNGC	6 cut(s)	143, 146, 397, 531, 547, 550
Bme18I	GGWCC	1 cut(s)	725
BmeT110I	CYCGRG	1 cut(s)	90
BmgT120I	GGNCC	2 cut(s)	725, 797
BmtI	GCTAGC	1 cut(s)	641
BpiI	GAAGAC	1 cut(s)	108
BpmI	CTGGAG	1 cut(s)	576
BpuEI	CTTGAG	1 cut(s)	64
BsaI	GGTCTC	1 cut(s)	498
BsaJI	CCNNGG	3 cut(s)	139, 183, 828
BsaXI	ACNNNNNCTCC	2 cut(s)	180, 210
Bsc4I	CCNNNNNNNGG	1 cut(s)	258
Bse1I	ACTGG	4 cut(s)	133, 434, 593, 705
BseDI	CCNNGG	3 cut(s)	139, 183, 828
BseGI	GGATG	2 cut(s)	171, 701
BseLI	CCNNNNNNNGG	1 cut(s)	258
BseMII	CTCAG	4 cut(s)	88, 519, 735, 798
BseNI	ACTGG	4 cut(s)	133, 434, 593, 705
BsePI	GCGCGC	1 cut(s)	247
BseRI	GAGGAG	2 cut(s)	91, 657
BseXI	GCAGC	2 cut(s)	541, 560
BseYI	CCCAGC	1 cut(s)	454
Bsh1236I	CGCG	3 cut(s)	141, 201, 249
Bsh1285I	CGRYCG	2 cut(s)	319, 421
BshFI	GGCC	2 cut(s)	147, 798
BsiEI	CGRYCG	2 cut(s)	319, 421
BsiHKAI	GWGCWC	2 cut(s)	50, 171
BsiHKCI	CYCGRG	1 cut(s)	90
BslFI	GGGAC	1 cut(s)	121
BslI	CCNNNNNNNGG	1 cut(s)	258
BsmAI	GTCTC	1 cut(s)	498
BsmFI	GGGAC	1 cut(s)	121
BsmI	GAATGC	1 cut(s)	95
BsnI	GGCC	2 cut(s)	147, 798
Bso31I	GGTCTC	1 cut(s)	498
BsoBI	CYCGRG	1 cut(s)	90
Bsp1286I	GDGCHC	2 cut(s)	50, 171
Bsp143I	GATC	1 cut(s)	349
BspACI	CCGC	9 cut(s)	139, 141, 144, 199, 319, 395, 417, 490, 545
BspANI	GGCC	2 cut(s)	147, 798
BspCNI	CTCAG	4 cut(s)	87, 518, 736, 797
BspFNI	CGCG	3 cut(s)	141, 201, 249
BspMAI	CTGCAG	1 cut(s)	658
BspOI	GCTAGC	1 cut(s)	641
BspPI	GGATC	1 cut(s)	344
BspQI	GCTCTTC	1 cut(s)	840
BspTNI	GGTCTC	1 cut(s)	498
BsrBI	CCGCTC	2 cut(s)	321, 490
BsrI	ACTGG	4 cut(s)	133, 434, 593, 705
BssECI	CCNNGG	3 cut(s)	139, 183, 828
BssHII	GCGCGC	1 cut(s)	247
BssMI	GATC	1 cut(s)	349
BssT1I	CCWWGG	1 cut(s)	183
Bst4CI	ACNGT	3 cut(s)	381, 630, 829
Bst6I	CTCTTC	2 cut(s)	109, 840
BstC8I	GCNNGC	2 cut(s)	249, 639
BstDEI	CTNAG	4 cut(s)	74, 505, 744, 784
BstDSI	CCRYGG	2 cut(s)	139, 828
BstF5I	GGATG	2 cut(s)	171, 701
BstFNI	CGCG	3 cut(s)	141, 201, 249
BstHHI	GCGC	2 cut(s)	249, 251
BstKTI	GATC	1 cut(s)	352
BstMAI	GTCTC	1 cut(s)	498
BstMBI	GATC	1 cut(s)	349
BstMCI	CGRYCG	2 cut(s)	319, 421
BstMWI	GCNNNNNNNGC	8 cut(s)	153, 198, 207, 318, 487, 647, 830, 839
BstNSI	RCATGY	2 cut(s)	389, 879
BstSFI	CTRYAG	1 cut(s)	654
BstUI	CGCG	3 cut(s)	141, 201, 249
BstV1I	GCAGC	2 cut(s)	541, 560
BstV2I	GAAGAC	1 cut(s)	108
BstX2I	RGATCY	1 cut(s)	349
BstYI	RGATCY	1 cut(s)	349
BsuRI	GGCC	2 cut(s)	147, 798
BtgI	CCRYGG	2 cut(s)	139, 828
BtsCI	GGATG	2 cut(s)	171, 701
BtsIMutI	CAGTG	4 cut(s)	386, 600, 712, 741
Cac8I	GCNNGC	2 cut(s)	249, 639
CfoI	GCGC	2 cut(s)	249, 251
Cfr13I	GGNCC	2 cut(s)	725, 797
Cfr42I	CCGCGG	1 cut(s)	142
CseI	GACGC	1 cut(s)	301
Csp6I	GTAC	2 cut(s)	203, 405
CviAII	CATG	6 cut(s)	226, 386, 541, 572, 850, 876
CviQI	GTAC	2 cut(s)	203, 405
DdeI	CTNAG	4 cut(s)	74, 505, 744, 784
DpnI	GATC	1 cut(s)	351
DpnII	GATC	1 cut(s)	349
DraIII	CACNNNGTG	1 cut(s)	381
DrdI	GACNNNNNNGTC	1 cut(s)	490
DseDI	GACNNNNNNGTC	1 cut(s)	490
Eam1104I	CTCTTC	2 cut(s)	109, 840
EarI	CTCTTC	2 cut(s)	109, 840
Ecl136II	GAGCTC	2 cut(s)	48, 169
Eco130I	CCWWGG	1 cut(s)	183
Eco24I	GRGCYC	2 cut(s)	50, 171
Eco31I	GGTCTC	1 cut(s)	498
Eco47I	GGWCC	1 cut(s)	725
Eco53kI	GAGCTC	2 cut(s)	48, 169
Eco57I	CTGAAG	1 cut(s)	102
Eco88I	CYCGRG	1 cut(s)	90
EcoICRI	GAGCTC	2 cut(s)	48, 169
EcoT14I	CCWWGG	1 cut(s)	183
EcoT38I	GRGCYC	2 cut(s)	50, 171
ErhI	CCWWGG	1 cut(s)	183
FaeI	CATG	6 cut(s)	229, 389, 544, 575, 853, 879
FaiI	YATR	8 cut(s)	227, 387, 542, 573, 584, 851, 856, 877
FaqI	GGGAC	1 cut(s)	121
FatI	CATG	6 cut(s)	225, 385, 540, 571, 849, 875
FauI	CCCGC	1 cut(s)	146
FblI	GTMKAC	1 cut(s)	315
Fnu4HI	GCNGC	6 cut(s)	142, 145, 396, 530, 546, 549
FokI	GGATG	2 cut(s)	158, 708
FriOI	GRGCYC	2 cut(s)	50, 171
Fsp4HI	GCNGC	6 cut(s)	142, 145, 396, 530, 546, 549
FspBI	CTAG	2 cut(s)	462, 638
GlaI	GCGC	2 cut(s)	248, 250
GluI	GCNGC	6 cut(s)	142, 145, 396, 530, 546, 549
GsaI	CCCAGC	1 cut(s)	458
GsuI	CTGGAG	1 cut(s)	576
HaeIII	GGCC	2 cut(s)	147, 798
HgaI	GACGC	1 cut(s)	301
HhaI	GCGC	2 cut(s)	249, 251
Hin1II	CATG	6 cut(s)	229, 389, 544, 575, 853, 879
Hin6I	GCGC	2 cut(s)	247, 249
HinP1I	GCGC	2 cut(s)	247, 249
HincII	GTYRAC	2 cut(s)	316, 538
HindII	GTYRAC	2 cut(s)	316, 538
HinfI	GANTC	3 cut(s)	586, 701, 863
HphI	GGTGA	2 cut(s)	746, 766
Hpy166II	GTNNAC	3 cut(s)	316, 520, 538
Hpy188I	TCNGA	5 cut(s)	77, 121, 166, 508, 862
Hpy188III	TCNNGA	3 cut(s)	43, 693, 885
Hpy8I	GTNNAC	3 cut(s)	316, 520, 538
Hpy99I	CGWCG	2 cut(s)	215, 317
HpyAV	CCTTC	2 cut(s)	406, 725
HpyCH4III	ACNGT	3 cut(s)	381, 630, 829
HpyCH4V	TGCA	8 cut(s)	4, 35, 447, 529, 626, 656, 680, 824
HpyF10VI	GCNNNNNNNGC	8 cut(s)	153, 198, 207, 318, 487, 647, 830, 839
HpyF3I	CTNAG	4 cut(s)	74, 505, 744, 784
Hsp92II	CATG	6 cut(s)	229, 389, 544, 575, 853, 879
HspAI	GCGC	2 cut(s)	247, 249
KspI	CCGCGG	1 cut(s)	142
Kzo9I	GATC	1 cut(s)	349
LguI	GCTCTTC	1 cut(s)	840
LmnI	GCTCC	1 cut(s)	451
Lsp1109I	GCAGC	2 cut(s)	541, 560
MaeI	CTAG	2 cut(s)	462, 638
MaeIII	GTNAC	1 cut(s)	734
MalI	GATC	1 cut(s)	351
MbiI	CCGCTC	2 cut(s)	321, 490
MboI	GATC	1 cut(s)	349
MboII	GAAGA	7 cut(s)	96, 99, 108, 151, 356, 359, 827
MflI	RGATCY	1 cut(s)	349
MhlI	GDGCHC	2 cut(s)	50, 171
MluCI	AATT	1 cut(s)	761
MlyI	GAGTC	1 cut(s)	710
MmeI	TCCRAC	2 cut(s)	153, 703
MseI	TTAA	3 cut(s)	302, 470, 683
MslI	CAYNNNNRTG	1 cut(s)	694
MspA1I	CMGCKG	1 cut(s)	141
Mva1269I	GAATGC	1 cut(s)	95
MvnI	CGCG	3 cut(s)	141, 201, 249
MwoI	GCNNNNNNNGC	8 cut(s)	153, 198, 207, 318, 487, 647, 830, 839
NdeII	GATC	1 cut(s)	349
NheI	GCTAGC	1 cut(s)	637
NlaIII	CATG	6 cut(s)	229, 389, 544, 575, 853, 879
NmuCI	GTSAC	1 cut(s)	734
NspI	RCATGY	2 cut(s)	389, 879
PaeR7I	CTCGAG	1 cut(s)	90
PauI	GCGCGC	1 cut(s)	247
PciSI	GCTCTTC	1 cut(s)	840
PctI	GAATGC	1 cut(s)	95
PfeI	GAWTC	2 cut(s)	586, 863
PkrI	GCNGC	6 cut(s)	143, 146, 397, 531, 547, 550
PleI	GAGTC	1 cut(s)	709
PpsI	GAGTC	1 cut(s)	709
Psp124BI	GAGCTC	2 cut(s)	50, 171
PspFI	CCCAGC	1 cut(s)	454
PspPI	GGNCC	2 cut(s)	725, 797
PspXI	VCTCGAGB	1 cut(s)	90
PstI	CTGCAG	1 cut(s)	658
PsuI	RGATCY	1 cut(s)	349
PteI	GCGCGC	1 cut(s)	247
RsaI	GTAC	2 cut(s)	204, 406
RsaNI	GTAC	2 cut(s)	203, 405
RseI	CAYNNNNRTG	1 cut(s)	694
SacI	GAGCTC	2 cut(s)	50, 171
SacII	CCGCGG	1 cut(s)	142
SalI	GTCGAC	1 cut(s)	314
SapI	GCTCTTC	1 cut(s)	840
SaqAI	TTAA	3 cut(s)	302, 470, 683
SatI	GCNGC	6 cut(s)	142, 145, 396, 530, 546, 549
Sau3AI	GATC	1 cut(s)	349
Sau96I	GGNCC	2 cut(s)	725, 797
SchI	GAGTC	1 cut(s)	710
SduI	GDGCHC	2 cut(s)	50, 171
SfcI	CTRYAG	1 cut(s)	654
Sfr274I	CTCGAG	1 cut(s)	90
Sfr303I	CCGCGG	1 cut(s)	142
SgrBI	CCGCGG	1 cut(s)	142
SinI	GGWCC	1 cut(s)	725
SlaI	CTCGAG	1 cut(s)	90
SmiMI	CAYNNNNRTG	1 cut(s)	694
SmlI	CTYRAG	2 cut(s)	43, 90
SmoI	CTYRAG	2 cut(s)	43, 90
Sse9I	AATT	1 cut(s)	761
SsiI	CCGC	9 cut(s)	139, 141, 144, 199, 319, 395, 417, 490, 545
SspMI	CTAG	2 cut(s)	462, 638
SstI	GAGCTC	2 cut(s)	50, 171
StyI	CCWWGG	1 cut(s)	183
TaaI	ACNGT	3 cut(s)	381, 630, 829
TaqI	TCGA	5 cut(s)	83, 91, 315, 421, 805
TasI	AATT	1 cut(s)	761
TauI	GCSGC	4 cut(s)	144, 147, 398, 548
TfiI	GAWTC	2 cut(s)	586, 863
Tru1I	TTAA	3 cut(s)	302, 470, 683
Tru9I	TTAA	3 cut(s)	302, 470, 683
TscAI	CASTG	4 cut(s)	386, 600, 712, 748
TseFI	GTSAC	1 cut(s)	734
TseI	GCWGC	2 cut(s)	529, 548
Tsp45I	GTSAC	1 cut(s)	734
TspDTI	ATGAA	3 cut(s)	588, 599, 678
TspGWI	ACGGA	1 cut(s)	464
TspRI	CASTG	4 cut(s)	386, 600, 712, 748
VpaK11BI	GGWCC	1 cut(s)	725
XceI	RCATGY	2 cut(s)	389, 879
XhoI	CTCGAG	1 cut(s)	90
XmiI	GTMKAC	1 cut(s)	315
XspI	CTAG	2 cut(s)	462, 638

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.