pycom10g10930

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0006752
Physical Location & Seq
Reverse (-)
14482527 .. 14484126
1600 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1026 bp
ATGAAGTTGCACTGTGGTTTTATTCGAAACGGTGGTGCTGAAATGGCTCCTGCAGACATCAAATATGTCAAAAAATGTAGATTTGTGGTTGCATCAGGCATTTTTGATGGTTATGATGTACCCCATCAACCTTCAAATTTAAGTCCTCGTTCTAAGAAGCTCTTCTGCTTGCTCATGGTGGTCGATGAAACATCTCTTGAATTTATCAAGGGAAATGTGACAGTCAGAGAGGATAATGACGGGGGAAAATGGGTTGGTATCTGGCGTCTTGTTTTGTTGAAGCATCCGCCTTACGATGAGCCAAGAAGGAACGGGAAGGTCCCCAAGATATTAACCCACCGCTTGTTCCCTAACGCCCAGTACAGCATTTGGATCGATGGTAAAATGGAGCTCATGGTTGATCCATTGCAAATTTTAGAAAGATATTTATGGCGTGGGAAGCATACATTTGCCATTTCTCAGCACAAGCACCACCACAGTATATACGAGGAGGCTGATGCAAACAAGCGGAGGAAGCGATATGCTCGACCTCTTATTGATCTACACATGAAAATTTATCGTTATGAGGGTTTGGAGCCATGGGATCCAACCAAGAAAACCGTTAGTGATGTACCAGAGGGTGCCATTATCATACGGGAACATACTGCGATGAGTAATTTATTTAGCTGCTTGTGGTTCAATGAGGTCCACCTCTTCACACCAAGAGACCAACTGAGTTTTGGTTATGTTGTGTACCGGTTAGGGGGGTTGTTTAAGTTCTTTATGTTTCCGAATTGTGAGTACAATTCCCTGTTTGTGTTGCATCCACACATAAGGGAGCATTCTTCTCGTATAGAGTGGGTAACAAGTTGGAATCAGCTCAAGGGGAATGGATCTATTTCAAATAAGGGTAAAGATTGGGACCAAGCCAGTGCGAAAAATGGTTTGGTAGAGAGTAGGGGAGGCTTAGGCTTGTGGACTCCTTACCCTGCAAATCTTGATTCAGTGGTCCTACCCTCGGTAGCAAGAACATCAAAAGCTGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616

Protein Analysis

342

Amino Acids

39.29

Weight (kDa)

9.38

Isoelectric Point (pI)

34.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 1 - 270 8.2e-119 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 620
AciI CCGC 3 cut(s) 287, 340, 508
AclWI GGATC 5 cut(s) 380, 395, 578, 591, 880
AcsI RAATTY 4 cut(s) 136, 200, 411, 552
AcyI GRCGYC 1 cut(s) 265
AfaI GTAC 5 cut(s) 120, 362, 612, 734, 782
AfiI CCNNNNNNNGG 2 cut(s) 742, 997
AgeI ACCGGT 1 cut(s) 735
AgsI TTSAA 5 cut(s) 135, 200, 280, 679, 882
AjuI GAANNNNNNNTTGG 2 cut(s) 908, 940
AluBI AGCT 5 cut(s) 160, 391, 666, 859, 1019
AluI AGCT 5 cut(s) 160, 391, 666, 859, 1019
Alw21I GWGCWC 1 cut(s) 393
Alw26I GTCTC 1 cut(s) 699
AlwI GGATC 5 cut(s) 380, 395, 578, 591, 880
ApeKI GCWGC 1 cut(s) 666
ApoI RAATTY 4 cut(s) 136, 200, 411, 552
AsiGI ACCGGT 1 cut(s) 735
Asp700I GAANNNNTTC 1 cut(s) 161
AspS9I GGNCC 4 cut(s) 319, 685, 901, 988
AsuII TTCGAA 1 cut(s) 25
AvaII GGWCC 4 cut(s) 319, 685, 901, 988
BamHI GGATCC 1 cut(s) 583
BanI GGYRCC 1 cut(s) 620
BanII GRGCYC 1 cut(s) 393
Bbv12I GWGCWC 1 cut(s) 393
BbvI GCAGC 1 cut(s) 653
BccI CCATC 3 cut(s) 101, 132, 371
BcgI CGANNNNNNTGC 4 cut(s) 355, 389, 809, 843
BcoDI GTCTC 1 cut(s) 699
BfmI CTRYAG 1 cut(s) 51
BisI GCNGC 1 cut(s) 667
BlsI GCNGC 1 cut(s) 668
Bme18I GGWCC 4 cut(s) 319, 685, 901, 988
BmgT120I GGNCC 4 cut(s) 319, 685, 901, 988
BmiI GGNNCC 6 cut(s) 48, 321, 576, 585, 622, 902
BmrI ACTGGG 1 cut(s) 352
BmsI GCATC 4 cut(s) 101, 292, 487, 811
BmuI ACTGGG 1 cut(s) 352
Bpu10I CCTNAGC 1 cut(s) 946
Bpu14I TTCGAA 1 cut(s) 25
BpuEI CTTGAG 1 cut(s) 845
Bsa29I ATCGAT 1 cut(s) 375
BsaHI GRCGYC 1 cut(s) 265
BsaI GGTCTC 1 cut(s) 699
BsaJI CCNNGG 2 cut(s) 578, 996
BsaWI WCCGGW 1 cut(s) 735
Bsc4I CCNNNNNNNGG 2 cut(s) 742, 997
Bse118I RCCGGY 1 cut(s) 735
Bse1I ACTGG 2 cut(s) 358, 909
Bse3DI GCAATG 1 cut(s) 404
BseCI ATCGAT 1 cut(s) 375
BseDI CCNNGG 2 cut(s) 578, 996
BseGI GGATG 2 cut(s) 283, 802
BseLI CCNNNNNNNGG 2 cut(s) 742, 997
BseMI GCAATG 1 cut(s) 404
BseMII CTCAG 2 cut(s) 473, 704
BseNI ACTGG 2 cut(s) 358, 909
BseRI GAGGAG 1 cut(s) 503
BseXI GCAGC 1 cut(s) 653
BshNI GGYRCC 1 cut(s) 620
BshTI ACCGGT 1 cut(s) 735
BshVI ATCGAT 1 cut(s) 375
BsiHKAI GWGCWC 1 cut(s) 393
BsiSI CCGG 1 cut(s) 736
BslFI GGGAC 2 cut(s) 305, 914
BslI CCNNNNNNNGG 2 cut(s) 742, 997
BsmAI GTCTC 1 cut(s) 699
BsmFI GGGAC 2 cut(s) 305, 914
BsmI GAATGC 1 cut(s) 820
Bso31I GGTCTC 1 cut(s) 699
Bsp119I TTCGAA 1 cut(s) 25
Bsp1286I GDGCHC 1 cut(s) 393
Bsp143I GATC 5 cut(s) 372, 400, 538, 583, 872
Bsp19I CCATGG 1 cut(s) 578
BspACI CCGC 3 cut(s) 287, 340, 508
BspCNI CTCAG 2 cut(s) 472, 705
BspDI ATCGAT 1 cut(s) 375
BspLI GGNNCC 6 cut(s) 48, 321, 576, 585, 622, 902
BspMAI CTGCAG 1 cut(s) 55
BspPI GGATC 5 cut(s) 380, 395, 578, 591, 880
BspQI GCTCTTC 1 cut(s) 167
BspT104I TTCGAA 1 cut(s) 25
BspT107I GGYRCC 1 cut(s) 620
BspTNI GGTCTC 1 cut(s) 699
BsrDI GCAATG 1 cut(s) 404
BsrFI RCCGGY 1 cut(s) 735
BsrI ACTGG 2 cut(s) 358, 909
BssAI RCCGGY 1 cut(s) 735
BssECI CCNNGG 2 cut(s) 578, 996
BssMI GATC 5 cut(s) 372, 400, 538, 583, 872
BssNI GRCGYC 1 cut(s) 265
BssT1I CCWWGG 1 cut(s) 578
Bst4CI ACNGT 5 cut(s) 14, 32, 223, 479, 601
Bst6I CTCTTC 2 cut(s) 167, 698
BstACI GRCGYC 1 cut(s) 265
BstBI TTCGAA 1 cut(s) 25
BstC8I GCNNGC 2 cut(s) 170, 1021
BstDEI CTNAG 4 cut(s) 153, 459, 713, 946
BstDSI CCRYGG 1 cut(s) 578
BstF5I GGATG 2 cut(s) 283, 802
BstKTI GATC 5 cut(s) 375, 403, 541, 586, 875
BstMAI GTCTC 1 cut(s) 699
BstMBI GATC 5 cut(s) 372, 400, 538, 583, 872
BstMWI GCNNNNNNNGC 3 cut(s) 44, 439, 514
BstSFI CTRYAG 1 cut(s) 51
BstV1I GCAGC 1 cut(s) 653
BstX2I RGATCY 2 cut(s) 583, 872
BstYI RGATCY 2 cut(s) 583, 872
Bsu15I ATCGAT 1 cut(s) 375
BsuTUI ATCGAT 1 cut(s) 375
BtgI CCRYGG 1 cut(s) 578
BtgZI GCGATG 1 cut(s) 662
BtsCI GGATG 2 cut(s) 283, 802
BtsIMutI CAGTG 3 cut(s) 10, 916, 990
Cac8I GCNNGC 2 cut(s) 170, 1021
Cfr10I RCCGGY 1 cut(s) 735
Cfr13I GGNCC 4 cut(s) 319, 685, 901, 988
ClaI ATCGAT 1 cut(s) 375
CseI GACGC 1 cut(s) 254
Csp6I GTAC 5 cut(s) 119, 361, 611, 733, 781
CspAI ACCGGT 1 cut(s) 735
CviAII CATG 4 cut(s) 175, 394, 547, 579
CviQI GTAC 5 cut(s) 119, 361, 611, 733, 781
DdeI CTNAG 4 cut(s) 153, 459, 713, 946
DpnI GATC 5 cut(s) 374, 402, 540, 585, 874
DpnII GATC 5 cut(s) 372, 400, 538, 583, 872
Eam1104I CTCTTC 2 cut(s) 167, 698
EarI CTCTTC 2 cut(s) 167, 698
EciI GGCGGA 1 cut(s) 276
Ecl136II GAGCTC 1 cut(s) 391
Eco130I CCWWGG 1 cut(s) 578
Eco24I GRGCYC 1 cut(s) 393
Eco31I GGTCTC 1 cut(s) 699
Eco47I GGWCC 4 cut(s) 319, 685, 901, 988
Eco53kI GAGCTC 1 cut(s) 391
EcoICRI GAGCTC 1 cut(s) 391
EcoO109I RGGNCCY 1 cut(s) 319
EcoT14I CCWWGG 1 cut(s) 578
EcoT38I GRGCYC 1 cut(s) 393
ErhI CCWWGG 1 cut(s) 578
FaeI CATG 4 cut(s) 178, 397, 550, 582
FalI AAGNNNNNCTT 2 cut(s) 146, 178
FaqI GGGAC 2 cut(s) 305, 914
FatI CATG 4 cut(s) 174, 393, 546, 578
Fnu4HI GCNGC 1 cut(s) 667
FokI GGATG 2 cut(s) 270, 789
FriOI GRGCYC 1 cut(s) 393
Fsp4HI GCNGC 1 cut(s) 667
GluI GCNGC 1 cut(s) 667
HapII CCGG 1 cut(s) 736
HgaI GACGC 1 cut(s) 254
Hin1I GRCGYC 1 cut(s) 265
Hin1II CATG 4 cut(s) 178, 397, 550, 582
HinfI GANTC 3 cut(s) 853, 958, 980
HpaII CCGG 1 cut(s) 736
Hpy166II GTNNAC 3 cut(s) 688, 733, 957
Hpy188I TCNGA 2 cut(s) 227, 771
Hpy188III TCNNGA 2 cut(s) 197, 977
Hpy8I GTNNAC 3 cut(s) 688, 733, 957
HpyAV CCTTC 3 cut(s) 141, 300, 310
HpyCH4III ACNGT 5 cut(s) 14, 32, 223, 479, 601
HpyCH4V TGCA 7 cut(s) 10, 53, 92, 409, 500, 802, 971
HpyF10VI GCNNNNNNNGC 3 cut(s) 44, 439, 514
HpyF3I CTNAG 4 cut(s) 153, 459, 713, 946
Hsp92I GRCGYC 1 cut(s) 265
Hsp92II CATG 4 cut(s) 178, 397, 550, 582
Kzo9I GATC 5 cut(s) 372, 400, 538, 583, 872
LguI GCTCTTC 1 cut(s) 167
LmnI GCTCC 4 cut(s) 52, 388, 574, 817
Lsp1109I GCAGC 1 cut(s) 653
LweI GCATC 4 cut(s) 101, 292, 487, 811
MaeIII GTNAC 2 cut(s) 217, 841
MalI GATC 5 cut(s) 374, 402, 540, 585, 874
MboI GATC 5 cut(s) 372, 400, 538, 583, 872
MboII GAAGA 3 cut(s) 154, 685, 816
MflI RGATCY 2 cut(s) 583, 872
MhlI GDGCHC 1 cut(s) 393
MluCI AATT 7 cut(s) 136, 200, 411, 552, 655, 772, 784
MlyI GAGTC 1 cut(s) 952
MmeI TCCRAC 2 cut(s) 611, 830
MroXI GAANNNNTTC 1 cut(s) 161
MseI TTAA 3 cut(s) 140, 332, 753
MspI CCGG 1 cut(s) 736
Mva1269I GAATGC 1 cut(s) 820
MwoI GCNNNNNNNGC 3 cut(s) 44, 439, 514
NcoI CCATGG 1 cut(s) 578
NdeII GATC 5 cut(s) 372, 400, 538, 583, 872
NlaIII CATG 4 cut(s) 178, 397, 550, 582
NlaIV GGNNCC 6 cut(s) 48, 321, 576, 585, 622, 902
NmuCI GTSAC 1 cut(s) 217
NspV TTCGAA 1 cut(s) 25
PciSI GCTCTTC 1 cut(s) 167
PctI GAATGC 1 cut(s) 820
PdmI GAANNNNTTC 1 cut(s) 161
PfeI GAWTC 2 cut(s) 853, 980
PinAI ACCGGT 1 cut(s) 735
PkrI GCNGC 1 cut(s) 668
PleI GAGTC 1 cut(s) 952
PpsI GAGTC 1 cut(s) 952
PpuMI RGGWCCY 1 cut(s) 319
Psp124BI GAGCTC 1 cut(s) 393
Psp5II RGGWCCY 1 cut(s) 319
PspN4I GGNNCC 6 cut(s) 48, 321, 576, 585, 622, 902
PspPI GGNCC 4 cut(s) 319, 685, 901, 988
PspPPI RGGWCCY 1 cut(s) 319
PstI CTGCAG 1 cut(s) 55
PsuI RGATCY 2 cut(s) 583, 872
RsaI GTAC 5 cut(s) 120, 362, 612, 734, 782
RsaNI GTAC 5 cut(s) 119, 361, 611, 733, 781
SacI GAGCTC 1 cut(s) 393
SapI GCTCTTC 1 cut(s) 167
SaqAI TTAA 3 cut(s) 140, 332, 753
SatI GCNGC 1 cut(s) 667
Sau3AI GATC 5 cut(s) 372, 400, 538, 583, 872
Sau96I GGNCC 4 cut(s) 319, 685, 901, 988
SchI GAGTC 1 cut(s) 952
SduI GDGCHC 1 cut(s) 393
SfaNI GCATC 4 cut(s) 101, 292, 487, 811
SfcI CTRYAG 1 cut(s) 51
SfuI TTCGAA 1 cut(s) 25
SinI GGWCC 4 cut(s) 319, 685, 901, 988
SmlI CTYRAG 1 cut(s) 860
SmoI CTYRAG 1 cut(s) 860
Sse9I AATT 7 cut(s) 136, 200, 411, 552, 655, 772, 784
SsiI CCGC 3 cut(s) 287, 340, 508
SstI GAGCTC 1 cut(s) 393
StyI CCWWGG 1 cut(s) 578
TaaI ACNGT 5 cut(s) 14, 32, 223, 479, 601
TaqI TCGA 4 cut(s) 25, 183, 375, 526
TasI AATT 7 cut(s) 136, 200, 411, 552, 655, 772, 784
TatI WGTACW 2 cut(s) 360, 780
TfiI GAWTC 2 cut(s) 853, 980
Tru1I TTAA 3 cut(s) 140, 332, 753
Tru9I TTAA 3 cut(s) 140, 332, 753
TscAI CASTG 3 cut(s) 17, 916, 990
TseFI GTSAC 1 cut(s) 217
TseI GCWGC 1 cut(s) 666
Tsp45I GTSAC 1 cut(s) 217
TspDTI ATGAA 3 cut(s) 17, 201, 563
TspRI CASTG 3 cut(s) 17, 916, 990
VpaK11BI GGWCC 4 cut(s) 319, 685, 901, 988
XapI RAATTY 4 cut(s) 136, 200, 411, 552
XcmI CCANNNNNNNNNTGG 1 cut(s) 716
XmnI GAANNNNTTC 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.