Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0013120

Physical Location & Seq

Genomic Coordinates Reverse (-)

16386957 .. 16388763

Gene Structure 1807 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom10g12910.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1689 bp

ATGAGTTACTCAGCGACAACGGTTACATTGAACTCAGCATTCCCGCGAAATTCAAATCCAAAAGCCAAGGATTCCCAAAACCGGAGAGAAACAATCTCTCTAATACAAAAATGCAAACACATTCATCAGATTCCGCCAATCCACGCAAAAATCATAAGAAATTTCGAAGACCAAGACCCATTCGTCGTCTTCGAGCTTCTTCGCCTCTGCTCCAACCTCAACTCAATCGACTACGCCACCAAGATTTTCCAACAAATTCAAACCCCAAATGTCTACCTCTACACTGCTCTCATTGATGGGTTCGTGTCGTCTGGGTTTTACCTCGATGCGATTCGGGTTTACTGTCGAATGGTTAACGGGTTTGTTTCGCCGGATAACTACGCGGTAACTTCTGTGTTAAAAGCTTGTGGCTTTGGGTTGGCGTTGGAGGAGGGGAGAGGAGTCCATGCGCAGGTTTTGAAGCTTGGGTTGTCATCGAATCGATCGGTGAGGATGAAGCTTCTCGATCTTTATGGGAAGAGTGGTGAATTTGAGTGTGCACGGAGGGTGTTCGATGAAATGCCTGATAGGGATGTCGTTGCGTCAACGGTGATGATTTCTTCTTATGCTGATTATGGGTTGATTGAGGAGGCGATTGGTGTTTTTAACCGGGCTAGGAGTAAGGATACGGTTTGTTGGACTGCGATGATTGATGGGTTGGTTAGGAATGGGGAGATGAATAGGGCTTTGGAGGCGTTTAGGGAGATGCAGAGGAATAATGTGAGGCCTAATGAAGTTACTGTTGTTTGTGTTTTATCTGCTTGTTCTTATTTGGGAGCATTGGAGCTCGGAAGATGGGTTCACTCTTACATAGATAAGTATGACATCAAATTCAATTACATAGTAGGTGGTGCTCTGATTAATATGTACTCGAGGTGTGGAGATATTGATGAGGCACTGGCAGTGTTTGGTAAGATGAAAGAGAGAGATGTGAGTAGTTATAATTCCATGATTGAGGGGCTTGCTATGCATGGGAAGAGCATGGAGGCTATCCAAATGTTCCAGACAATGATAAAACAAGGACTAAGGCCAAATAGCATTACTTTTGTTAAAGTATTGAATGCATGTAGTCATGGAGGTTTGTTGGGTTTGGGTTTTCAGATATTTCATTCCATGACTAATATTCACGGCATTCAACCACAGATAGAACACTATGGATGCATGGTTGATCGTTCTAGGTCGCTCAGGGTATCCAAAATCTTGGTGAATTGTGGTAATGCAGATTCGGGAACTTACGTACTGCTATCAAATGTATATGCTTCATCAGGGAGGTGGAAAGAGGCTGCACAAGTGAGAGCAGAGATGAAGGAAGGTGGAACCCCAAAGGAGCCTGGTTGTAGCTTGATTGAAGTAAACAATGAGATCCATGAGTTCCCCTTGGGAGACCTCAGACACCCGAAGAGGGAGGAAATCTACAAAAAGTTAACGGAGCTAATCCATGAACTGAAATCAGAAGGATACTTGCCTGCAACAGAGGCGGTTTTGCATGATATTGAAGATACGCAAAAGGAATTGGCTCTATCGATACATAGCGAGAGGCTTGCCATATGCTACGGGCTAATTGCTACAAAACAAGGTACGACTTTGAGGATTGTAAATAACTTAAGGGTTTGCAGTGACTGCCACTCAATGATCGATCAAAAAAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0016070 GO:0016787 GO:0016788 GO:0031425 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1900865 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

563

Amino Acids

63.01

Weight (kDa)

7.53

Isoelectric Point (pI)

43.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	89 - 136	3.8e-07	PPR repeat family
PPR_2	PF13041	221 - 268	5.1e-13	PPR repeat family
PPR	PF01535	224 - 254	7.2e-08	PPR repeat
PPR	PF01535	298 - 324	4.6e-06	PPR repeat
PPR_2	PF13041	322 - 370	2.3e-11	PPR repeat family
PPR_3	PF13812	323 - 369	7.4e-06	Pentatricopeptide repeat domain
PPR	PF01535	326 - 355	3.4e-06	PPR repeat
E_motif	PF20431	412 - 463	2.5e-16	E motif
DYW_deaminase	PF14432	499 - 558	2.5e-19	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0013120)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G59200
fragaria_vesca	FvH4_2g17580
malus_domestica	MD10G1149200.v1.1 MD10G1149700.v1.1
prunus_persica	Prupe.8G188000_v2.0.a1
pyrus_communis	pycom10g12880 pycom10g12910
rosa_chinensis	RchiOBHm_Chr6g0282521
rosa_laevigata	RLG00000012888
rosa_multiflora	Rmu_sc0000283.1_g000009 Rmu_sc0000283.1_g000011 Rmu_sc0000283.1_g000012 Rmu_sc0005697.1_g000029
rosa_rugosa	Rorug06G0147100 Rorug06G0147200
rosa_samantha	Rh6CG262700 Rh6DG254600
rosa_wichuraiana	Rw6G022520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	981
AasI	GACNNNNNNGTC	1 cut(s)	182
Acc16I	TGCGCA	1 cut(s)	450
Acc36I	ACCTGC	1 cut(s)	442
AccI	GTMKAC	1 cut(s)	273
AccII	CGCG	2 cut(s)	46, 383
AciI	CCGC	4 cut(s)	44, 134, 383, 1517
AclWI	GGATC	1 cut(s)	1396
AcsI	RAATTY	5 cut(s)	49, 160, 255, 527, 869
AfaI	GTAC	3 cut(s)	908, 1278, 1618
AfiI	CCNNNNNNNGG	3 cut(s)	81, 451, 1441
AflII	CTTAAG	1 cut(s)	1642
AgsI	TTSAA	9 cut(s)	31, 54, 260, 460, 874, 1099, 1175, 1388, 1535
AjnI	CCWGG	1 cut(s)	1369
AjuI	GAANNNNNNNTTGG	2 cut(s)	710, 742
AloI	GAACNNNNNNTCC	2 cut(s)	822, 854
AluBI	AGCT	7 cut(s)	196, 404, 463, 499, 826, 1380, 1471
AluI	AGCT	7 cut(s)	196, 404, 463, 499, 826, 1380, 1471
Alw21I	GWGCWC	3 cut(s)	541, 828, 895
Alw26I	GTCTC	1 cut(s)	1416
Alw44I	GTGCAC	1 cut(s)	537
AlwI	GGATC	1 cut(s)	1396
AlwNI	CAGNNNCTG	1 cut(s)	1659
Ama87I	CYCGRG	1 cut(s)	910
AoxI	GGCC	2 cut(s)	764, 1067
ApaLI	GTGCAC	1 cut(s)	537
ApeKI	GCWGC	1 cut(s)	1322
ApoI	RAATTY	5 cut(s)	49, 160, 255, 527, 869
AseI	ATTAAT	1 cut(s)	900
AspLEI	GCGC	1 cut(s)	451
AsuC2I	CCSGG	1 cut(s)	650
AsuHPI	GGTGA	4 cut(s)	499, 536, 601, 1255
AsuII	TTCGAA	1 cut(s)	165
AvaI	CYCGRG	1 cut(s)	910
BaeGI	GKGCMC	1 cut(s)	541
BanII	GRGCYC	1 cut(s)	828
BbsI	GAAGAC	2 cut(s)	174, 181
Bbv12I	GWGCWC	3 cut(s)	541, 828, 895
BbvI	GCAGC	1 cut(s)	1309
BccI	CCATC	3 cut(s)	290, 686, 828
BceAI	ACGGC	1 cut(s)	1183
BcgI	CGANNNNNNTGC	2 cut(s)	1562, 1596
BciT130I	CCWGG	1 cut(s)	1371
BciVI	GTATCC	3 cut(s)	658, 1240, 1490
BcnI	CCSGG	1 cut(s)	650
BcoDI	GTCTC	1 cut(s)	1416
BfaI	CTAG	2 cut(s)	654, 1215
BfrI	CTTAAG	1 cut(s)	1642
BfuAI	ACCTGC	1 cut(s)	442
BfuI	GTATCC	3 cut(s)	658, 1240, 1490
BisI	GCNGC	1 cut(s)	1323
BlsI	GCNGC	1 cut(s)	1324
Bme1390I	CCNGG	2 cut(s)	650, 1371
BmeT110I	CYCGRG	1 cut(s)	910
BmiI	GGNNCC	2 cut(s)	1357, 1368
BmrFI	CCNGG	2 cut(s)	650, 1371
BmsI	GCATC	3 cut(s)	316, 735, 1187
BpiI	GAAGAC	2 cut(s)	174, 181
Bpu10I	CCTNAGC	1 cut(s)	1223
Bpu14I	TTCGAA	1 cut(s)	165
BpuMI	CCSGG	1 cut(s)	650
Bsa29I	ATCGAT	3 cut(s)	481, 1562, 1674
BsaAI	YACGTR	1 cut(s)	1276
BsaI	GGTCTC	1 cut(s)	1416
BsaJI	CCNNGG	2 cut(s)	66, 1416
BsaWI	WCCGGW	1 cut(s)	81
Bsc4I	CCNNNNNNNGG	3 cut(s)	81, 451, 1441
Bse1I	ACTGG	1 cut(s)	942
BseBI	CCWGG	1 cut(s)	1371
BseCI	ATCGAT	3 cut(s)	481, 1562, 1674
BseDI	CCNNGG	2 cut(s)	66, 1416
BseGI	GGATG	3 cut(s)	498, 577, 1202
BseLI	CCNNNNNNNGG	3 cut(s)	81, 451, 1441
BseMII	CTCAG	4 cut(s)	24, 48, 1237, 1441
BseNI	ACTGG	1 cut(s)	942
BseRI	GAGGAG	3 cut(s)	443, 453, 641
BseSI	GKGCMC	1 cut(s)	541
BseXI	GCAGC	1 cut(s)	1309
BsgI	GTGCAG	1 cut(s)	1308
Bsh1236I	CGCG	2 cut(s)	46, 383
Bsh1285I	CGRYCG	1 cut(s)	485
BshFI	GGCC	2 cut(s)	766, 1069
BshVI	ATCGAT	3 cut(s)	481, 1562, 1674
BsiEI	CGRYCG	1 cut(s)	485
BsiHKAI	GWGCWC	3 cut(s)	541, 828, 895
BsiHKCI	CYCGRG	1 cut(s)	910
BsiSI	CCGG	3 cut(s)	82, 371, 649
BslI	CCNNNNNNNGG	3 cut(s)	81, 451, 1441
BsmAI	GTCTC	1 cut(s)	1416
BsmI	GAATGC	3 cut(s)	38, 1105, 1170
BsnI	GGCC	2 cut(s)	766, 1069
Bso31I	GGTCTC	1 cut(s)	1416
BsoBI	CYCGRG	1 cut(s)	910
Bsp119I	TTCGAA	1 cut(s)	165
Bsp1286I	GDGCHC	3 cut(s)	541, 828, 895
Bsp143I	GATC	6 cut(s)	482, 505, 1207, 1401, 1671, 1675
BspACI	CCGC	4 cut(s)	44, 134, 383, 1517
BspANI	GGCC	2 cut(s)	766, 1069
BspCNI	CTCAG	4 cut(s)	23, 47, 1236, 1440
BspDI	ATCGAT	3 cut(s)	481, 1562, 1674
BspFNI	CGCG	2 cut(s)	46, 383
BspLI	GGNNCC	2 cut(s)	1357, 1368
BspMI	ACCTGC	1 cut(s)	442
BspPI	GGATC	1 cut(s)	1396
BspQI	GCTCTTC	1 cut(s)	1010
BspT104I	TTCGAA	1 cut(s)	165
BspTI	CTTAAG	1 cut(s)	1642
BspTNI	GGTCTC	1 cut(s)	1416
BsrI	ACTGG	1 cut(s)	942
BssECI	CCNNGG	2 cut(s)	66, 1416
BssMI	GATC	6 cut(s)	482, 505, 1207, 1401, 1671, 1675
BssT1I	CCWWGG	2 cut(s)	66, 1416
Bst2UI	CCWGG	1 cut(s)	1371
Bst4CI	ACNGT	5 cut(s)	22, 344, 589, 670, 781
Bst6I	CTCTTC	3 cut(s)	512, 1010, 1433
BstAFI	CTTAAG	1 cut(s)	1642
BstAPI	GCANNNNNTGC	1 cut(s)	1659
BstBAI	YACGTR	1 cut(s)	1276
BstBI	TTCGAA	1 cut(s)	165
BstC8I	GCNNGC	3 cut(s)	1002, 1506, 1581
BstDEI	CTNAG	5 cut(s)	10, 34, 1064, 1223, 1427
BstF5I	GGATG	3 cut(s)	498, 577, 1202
BstFNI	CGCG	2 cut(s)	46, 383
BstHHI	GCGC	1 cut(s)	451
BstKTI	GATC	6 cut(s)	485, 508, 1210, 1404, 1674, 1678
BstMAI	GTCTC	1 cut(s)	1416
BstMBI	GATC	6 cut(s)	482, 505, 1207, 1401, 1671, 1675
BstMCI	CGRYCG	1 cut(s)	485
BstMWI	GCNNNNNNNGC	4 cut(s)	731, 1006, 1514, 1659
BstNI	CCWGG	1 cut(s)	1371
BstNSI	RCATGY	1 cut(s)	1107
BstSCI	CCNGG	2 cut(s)	648, 1369
BstSLI	GKGCMC	1 cut(s)	541
BstSNI	TACGTA	1 cut(s)	1276
BstUI	CGCG	2 cut(s)	46, 383
BstV1I	GCAGC	1 cut(s)	1309
BstV2I	GAAGAC	2 cut(s)	174, 181
BstX2I	RGATCY	1 cut(s)	1401
BstXI	CCANNNNNNTGG	1 cut(s)	1240
BstYI	RGATCY	1 cut(s)	1401
Bsu15I	ATCGAT	3 cut(s)	481, 1562, 1674
BsuI	GTATCC	3 cut(s)	658, 1240, 1490
BsuRI	GGCC	2 cut(s)	766, 1069
BsuTUI	ATCGAT	3 cut(s)	481, 1562, 1674
BtgZI	GCGATG	1 cut(s)	698
BtsCI	GGATG	3 cut(s)	498, 577, 1202
BtsI	GCAGTG	3 cut(s)	282, 948, 1660
BtsIMutI	CAGTG	4 cut(s)	282, 935, 948, 1660
BveI	ACCTGC	1 cut(s)	442
Cac8I	GCNNGC	3 cut(s)	1002, 1506, 1581
CaiI	CAGNNNCTG	1 cut(s)	1659
CfoI	GCGC	1 cut(s)	451
ClaI	ATCGAT	3 cut(s)	481, 1562, 1674
CseI	GACGC	1 cut(s)	570
Csp6I	GTAC	3 cut(s)	907, 1277, 1617
CviQI	GTAC	3 cut(s)	907, 1277, 1617
DdeI	CTNAG	5 cut(s)	10, 34, 1064, 1223, 1427
DpnI	GATC	6 cut(s)	484, 507, 1209, 1403, 1673, 1677
DpnII	GATC	6 cut(s)	482, 505, 1207, 1401, 1671, 1675
DrdI	GACNNNNNNGTC	1 cut(s)	182
DseDI	GACNNNNNNGTC	1 cut(s)	182
Eam1104I	CTCTTC	3 cut(s)	512, 1010, 1433
EarI	CTCTTC	3 cut(s)	512, 1010, 1433
EciI	GGCGGA	1 cut(s)	123
Ecl136II	GAGCTC	1 cut(s)	826
Eco105I	TACGTA	1 cut(s)	1276
Eco130I	CCWWGG	2 cut(s)	66, 1416
Eco147I	AGGCCT	1 cut(s)	766
Eco24I	GRGCYC	1 cut(s)	828
Eco31I	GGTCTC	1 cut(s)	1416
Eco53kI	GAGCTC	1 cut(s)	826
Eco88I	CYCGRG	1 cut(s)	910
EcoICRI	GAGCTC	1 cut(s)	826
EcoRII	CCWGG	1 cut(s)	1369
EcoT14I	CCWWGG	2 cut(s)	66, 1416
EcoT22I	ATGCAT	3 cut(s)	1011, 1105, 1202
EcoT38I	GRGCYC	1 cut(s)	828
ErhI	CCWWGG	2 cut(s)	66, 1416
FauI	CCCGC	1 cut(s)	51
FauNDI	CATATG	1 cut(s)	1586
FblI	GTMKAC	1 cut(s)	273
Fnu4HI	GCNGC	1 cut(s)	1323
FokI	GGATG	3 cut(s)	505, 584, 1209
FriOI	GRGCYC	1 cut(s)	828
Fsp4HI	GCNGC	1 cut(s)	1323
FspBI	CTAG	2 cut(s)	654, 1215
FspI	TGCGCA	1 cut(s)	450
GlaI	GCGC	1 cut(s)	450
GluI	GCNGC	1 cut(s)	1323
HaeIII	GGCC	2 cut(s)	766, 1069
HapII	CCGG	3 cut(s)	82, 371, 649
HgaI	GACGC	1 cut(s)	570
HhaI	GCGC	1 cut(s)	451
Hin6I	GCGC	1 cut(s)	449
HinP1I	GCGC	1 cut(s)	449
HincII	GTYRAC	3 cut(s)	355, 585, 1464
HindII	GTYRAC	3 cut(s)	355, 585, 1464
HindIII	AAGCTT	3 cut(s)	402, 461, 497
HinfI	GANTC	6 cut(s)	71, 130, 331, 441, 478, 1262
HpaI	GTTAAC	2 cut(s)	355, 1464
HpaII	CCGG	3 cut(s)	82, 371, 649
HphI	GGTGA	4 cut(s)	499, 536, 601, 1255
Hpy166II	GTNNAC	8 cut(s)	274, 340, 355, 539, 585, 841, 1393, 1464
Hpy188I	TCNGA	6 cut(s)	129, 830, 897, 1140, 1430, 1492
Hpy188III	TCNNGA	3 cut(s)	503, 1042, 1266
Hpy8I	GTNNAC	8 cut(s)	274, 340, 355, 539, 585, 841, 1393, 1464
Hpy99I	CGWCG	1 cut(s)	188
HpyAV	CCTTC	3 cut(s)	1339, 1343, 1487
HpyCH4III	ACNGT	5 cut(s)	22, 344, 589, 670, 781
HpyCH4IV	ACGT	1 cut(s)	1275
HpyF10VI	GCNNNNNNNGC	4 cut(s)	731, 1006, 1514, 1659
HpyF3I	CTNAG	5 cut(s)	10, 34, 1064, 1223, 1427
HpySE526I	ACGT	1 cut(s)	1275
HspAI	GCGC	1 cut(s)	449
KspAI	GTTAAC	2 cut(s)	355, 1464
Kzo9I	GATC	6 cut(s)	482, 505, 1207, 1401, 1671, 1675
LguI	GCTCTTC	1 cut(s)	1010
LmnI	GCTCC	5 cut(s)	215, 815, 823, 1366, 1468
Lsp1109I	GCAGC	1 cut(s)	1309
LweI	GCATC	3 cut(s)	316, 735, 1187
MaeI	CTAG	2 cut(s)	654, 1215
MaeII	ACGT	1 cut(s)	1275
MaeIII	GTNAC	5 cut(s)	5, 22, 385, 775, 1655
MalI	GATC	6 cut(s)	484, 507, 1209, 1403, 1673, 1677
MboI	GATC	6 cut(s)	482, 505, 1207, 1401, 1671, 1675
MboII	GAAGA	9 cut(s)	179, 181, 191, 529, 591, 843, 1027, 1450, 1547
MflI	RGATCY	1 cut(s)	1401
MhlI	GDGCHC	3 cut(s)	541, 828, 895
MlyI	GAGTC	1 cut(s)	450
MmeI	TCCRAC	4 cut(s)	237, 274, 405, 656
Mph1103I	ATGCAT	3 cut(s)	1011, 1105, 1202
MseI	TTAA	7 cut(s)	354, 398, 645, 900, 1089, 1463, 1643
MspCI	CTTAAG	1 cut(s)	1642
MspI	CCGG	3 cut(s)	82, 371, 649
MspR9I	CCNGG	2 cut(s)	650, 1371
Mva1269I	GAATGC	3 cut(s)	38, 1105, 1170
MvaI	CCWGG	1 cut(s)	1371
MvnI	CGCG	2 cut(s)	46, 383
MwoI	GCNNNNNNNGC	4 cut(s)	731, 1006, 1514, 1659
NciI	CCSGG	1 cut(s)	650
NdeI	CATATG	1 cut(s)	1586
NdeII	GATC	6 cut(s)	482, 505, 1207, 1401, 1671, 1675
NlaIV	GGNNCC	2 cut(s)	1357, 1368
NmuCI	GTSAC	1 cut(s)	1655
NsbI	TGCGCA	1 cut(s)	450
NsiI	ATGCAT	3 cut(s)	1011, 1105, 1202
NspI	RCATGY	1 cut(s)	1107
NspV	TTCGAA	1 cut(s)	165
PaeR7I	CTCGAG	1 cut(s)	910
PceI	AGGCCT	1 cut(s)	766
PciSI	GCTCTTC	1 cut(s)	1010
PcsI	WCGNNNNNNNCGW	1 cut(s)	189
PctI	GAATGC	3 cut(s)	38, 1105, 1170
PfeI	GAWTC	5 cut(s)	71, 130, 331, 478, 1262
PkrI	GCNGC	1 cut(s)	1324
Ple19I	CGATCG	1 cut(s)	485
PleI	GAGTC	1 cut(s)	449
PpsI	GAGTC	1 cut(s)	449
Ppu21I	YACGTR	1 cut(s)	1276
PshBI	ATTAAT	1 cut(s)	900
PsiI	TTATAA	1 cut(s)	981
Psp124BI	GAGCTC	1 cut(s)	828
Psp6I	CCWGG	1 cut(s)	1369
PspGI	CCWGG	1 cut(s)	1369
PspN4I	GGNNCC	2 cut(s)	1357, 1368
PspXI	VCTCGAGB	1 cut(s)	910
PstNI	CAGNNNCTG	1 cut(s)	1659
PsuI	RGATCY	1 cut(s)	1401
PvuI	CGATCG	1 cut(s)	485
RsaI	GTAC	3 cut(s)	908, 1278, 1618
RsaNI	GTAC	3 cut(s)	907, 1277, 1617
SacI	GAGCTC	1 cut(s)	828
SapI	GCTCTTC	1 cut(s)	1010
SaqAI	TTAA	7 cut(s)	354, 398, 645, 900, 1089, 1463, 1643
SatI	GCNGC	1 cut(s)	1323
Sau3AI	GATC	6 cut(s)	482, 505, 1207, 1401, 1671, 1675
SchI	GAGTC	1 cut(s)	450
ScrFI	CCNGG	2 cut(s)	650, 1371
SduI	GDGCHC	3 cut(s)	541, 828, 895
SfaNI	GCATC	3 cut(s)	316, 735, 1187
Sfr274I	CTCGAG	1 cut(s)	910
SfuI	TTCGAA	1 cut(s)	165
SlaI	CTCGAG	1 cut(s)	910
SmlI	CTYRAG	2 cut(s)	910, 1642
SmoI	CTYRAG	2 cut(s)	910, 1642
SnaBI	TACGTA	1 cut(s)	1276
SseBI	AGGCCT	1 cut(s)	766
SsiI	CCGC	4 cut(s)	44, 134, 383, 1517
SspI	AATATT	1 cut(s)	1162
SspMI	CTAG	2 cut(s)	654, 1215
SstI	GAGCTC	1 cut(s)	828
StuI	AGGCCT	1 cut(s)	766
StyD4I	CCNGG	2 cut(s)	648, 1369
StyI	CCWWGG	2 cut(s)	66, 1416
TaaI	ACNGT	5 cut(s)	22, 344, 589, 670, 781
TaiI	ACGT	1 cut(s)	1278
TatI	WGTACW	1 cut(s)	906
TfiI	GAWTC	5 cut(s)	71, 130, 331, 478, 1262
Tru1I	TTAA	7 cut(s)	354, 398, 645, 900, 1089, 1463, 1643
Tru9I	TTAA	7 cut(s)	354, 398, 645, 900, 1089, 1463, 1643
TscAI	CASTG	4 cut(s)	289, 942, 948, 1660
TseFI	GTSAC	1 cut(s)	1655
TseI	GCWGC	1 cut(s)	1322
Tsp45I	GTSAC	1 cut(s)	1655
TspGWI	ACGGA	2 cut(s)	556, 1481
TspRI	CASTG	4 cut(s)	289, 942, 948, 1660
Vha464I	CTTAAG	1 cut(s)	1642
VneI	GTGCAC	1 cut(s)	537
VspI	ATTAAT	1 cut(s)	900
XapI	RAATTY	5 cut(s)	49, 160, 255, 527, 869
XceI	RCATGY	1 cut(s)	1107
XhoI	CTCGAG	1 cut(s)	910
XmiI	GTMKAC	1 cut(s)	273
XspI	CTAG	2 cut(s)	654, 1215
Zsp2I	ATGCAT	3 cut(s)	1011, 1105, 1202

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.