pycom10g13110

At5g48480

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0011320
Physical Location & Seq
Forward (+)
16582091 .. 16583239
1149 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 795 bp
ATGGTCAATATCTTAATCCAACGGTCCAAAGTACATAAGCCGAAACGGGGAGTGGTACCATTCGTAATTCCTCTCAAACTCACGGGCAACTGTAAGACATCAACAGTCTTAATTTCCCGTTATACCCTCAACCGTACGGTTCCCCCACGCCTTTTGCCTTTTCCGTCTTCTGCTCTCTTCTCTCGGCGTCCTCCTCTCTTTCTCTCTCTCCTAGGGTTAGGGTTTCTCTGTCCTTTCTCAACATTTGCTCTCACTCTTTGTGCTTCAATGGAGGAGCAAAAGGTACAGAAAGGCGTGGCCGCCGAGAAGGTCGCACCCGTCTCCCCCATCTCTGTCACGGAGTGCTACCCTCGTCTCTCGATCAAGGCACCCAAGGGTGAAGACGCCATCAAGTTCTACGAGACCGTCTTCGGCGCCGTGGAGCTTGAGCGTAAGAGTGATCTCAAGCGCAAGGCTGACCAGGAGCGTCCCTTCATCTACAATGCTAAGCTCAAGCTTGGGTCCTTCACCTTCCTTGTGACTGAGTCTGACTCGATTGTTGACTCCGGTGACGCCGCAGCTCTGGCTGACGGTGGTTTTAAGCATCTGTTGCTTACTGTTGCGGACGTCGATGCGGCTGTGTCCAAGGCCGTCGAGGCGGGAGCTGCGGCGGTTGGGGAGATCAAAGCTGGTGGTGCTTTCTTTGGCGACGAGCAGCGTTGGGCACAGATTAAGGATCCTTTCGGCATTTACTGGTTCTTCGTCACCCCCCTTGAGAAGAAGGCCGCTATGGCCGAAGCCCCGGTTACCGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3-dmu-9_3-mt Glyoxalase

Protein Analysis

265

Amino Acids

28.68

Weight (kDa)

9.28

Isoelectric Point (pI)

39.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At5g48480-like_N PF22656 119 - 174 6.5e-20 Glyoxalase At5g48480-like, N-terminal domain
Glyoxalase PF00903 127 - 245 2.1e-07 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011320)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48480
fragaria_vesca FvH4_2g17810
malus_domestica MD05G1159600.v1.1 MD10G1152100.v1.1
prunus_persica Prupe.8G190400_v2.0.a1
pyrus_communis pycom05g15120 pycom10g13110
rosa_chinensis RchiOBHm_Chr6g0282841
rosa_laevigata RLG00000012864 RLG00000021743
rosa_multiflora Rmu_sc0006070.1_g000011
rosa_roxburghii Rroxscaffold_7G00185960
rosa_rugosa Rorug02G0530700 Rorug06G0149900
rosa_samantha Rh2BG610300 Rh6AG263200 Rh6BG266100 Rh6CG265300 Rh6DG257900
rosa_wichuraiana Rw6G022720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 609
Acc65I GGTACC 1 cut(s) 55
AccB1I GGYRCC 3 cut(s) 55, 367, 413
AciI CCGC 8 cut(s) 300, 555, 602, 614, 638, 647, 650, 765
AclWI GGATC 2 cut(s) 710, 723
AcoI YGGCCR 2 cut(s) 297, 771
AcyI GRCGYC 5 cut(s) 187, 384, 414, 552, 606
AdeI CACNNNGTG 1 cut(s) 342
AfaI GTAC 4 cut(s) 33, 57, 136, 285
AfiI CCNNNNNNNGG 1 cut(s) 47
AgsI TTSAA 1 cut(s) 267
AjnI CCWGG 1 cut(s) 459
AluBI AGCT 6 cut(s) 424, 490, 496, 560, 644, 668
AluI AGCT 6 cut(s) 424, 490, 496, 560, 644, 668
Alw26I GTCTC 3 cut(s) 325, 359, 395
AlwI GGATC 2 cut(s) 710, 723
AoxI GGCC 4 cut(s) 297, 627, 762, 771
ApeKI GCWGC 3 cut(s) 557, 644, 694
Asp718I GGTACC 1 cut(s) 55
AspA2I CCTAGG 1 cut(s) 211
AspLEI GCGC 2 cut(s) 416, 450
AspS9I GGNCC 2 cut(s) 24, 501
AsuC2I CCSGG 1 cut(s) 782
AsuHPI GGTGA 4 cut(s) 389, 499, 560, 736
AvaII GGWCC 2 cut(s) 24, 501
AvrII CCTAGG 1 cut(s) 211
BaeGI GKGCMC 1 cut(s) 706
BamHI GGATCC 1 cut(s) 715
BanI GGYRCC 3 cut(s) 55, 367, 413
BbsI GAAGAC 3 cut(s) 159, 387, 400
BbvI GCAGC 3 cut(s) 569, 631, 706
BccI CCATC 2 cut(s) 335, 395
BceAI ACGGC 2 cut(s) 401, 614
BciT130I CCWGG 1 cut(s) 461
BcnI CCSGG 1 cut(s) 782
BcoDI GTCTC 3 cut(s) 325, 359, 395
BfaI CTAG 1 cut(s) 212
BfoI RGCGCY 1 cut(s) 417
BglI GCCNNNNNGGC 2 cut(s) 635, 770
BisI GCNGC 8 cut(s) 300, 555, 558, 615, 645, 648, 695, 765
BlnI CCTAGG 1 cut(s) 211
BlpI GCTNAGC 1 cut(s) 486
BlsI GCNGC 8 cut(s) 301, 556, 559, 616, 646, 649, 696, 766
Bme1390I CCNGG 2 cut(s) 461, 782
Bme18I GGWCC 2 cut(s) 24, 501
BmgT120I GGNCC 2 cut(s) 24, 501
BmiI GGNNCC 6 cut(s) 57, 141, 369, 415, 502, 717
BmrFI CCNGG 2 cut(s) 461, 782
BmsI GCATC 2 cut(s) 592, 601
BpiI GAAGAC 3 cut(s) 159, 387, 400
BplI GAGNNNNNCTC 2 cut(s) 515, 547
Bpu1102I GCTNAGC 1 cut(s) 486
BpuEI CTTGAG 4 cut(s) 428, 446, 476, 773
BpuMI CCSGG 1 cut(s) 782
BsaHI GRCGYC 5 cut(s) 187, 384, 414, 552, 606
BsaI GGTCTC 1 cut(s) 395
BsaJI CCNNGG 5 cut(s) 211, 372, 417, 624, 780
BsaWI WCCGGW 1 cut(s) 545
Bsc4I CCNNNNNNNGG 1 cut(s) 47
Bse1I ACTGG 1 cut(s) 737
BseBI CCWGG 1 cut(s) 461
BseDI CCNNGG 5 cut(s) 211, 372, 417, 624, 780
BseLI CCNNNNNNNGG 1 cut(s) 47
BseMII CTCAG 1 cut(s) 513
BseNI ACTGG 1 cut(s) 737
BseRI GAGGAG 2 cut(s) 183, 287
BseSI GKGCMC 1 cut(s) 706
BseXI GCAGC 3 cut(s) 569, 631, 706
BshFI GGCC 4 cut(s) 299, 629, 764, 773
BshNI GGYRCC 3 cut(s) 55, 367, 413
BsiSI CCGG 2 cut(s) 546, 782
BsiWI CGTACG 1 cut(s) 134
BslFI GGGAC 1 cut(s) 453
BslI CCNNNNNNNGG 1 cut(s) 47
BsmAI GTCTC 3 cut(s) 325, 359, 395
BsmBI CGTCTC 2 cut(s) 325, 359
BsmFI GGGAC 1 cut(s) 453
BsnI GGCC 4 cut(s) 299, 629, 764, 773
Bso31I GGTCTC 1 cut(s) 395
Bsp1286I GDGCHC 1 cut(s) 706
Bsp143I GATC 4 cut(s) 360, 439, 660, 715
Bsp1720I GCTNAGC 1 cut(s) 486
BspACI CCGC 8 cut(s) 300, 555, 602, 614, 638, 647, 650, 765
BspANI GGCC 4 cut(s) 299, 629, 764, 773
BspCNI CTCAG 1 cut(s) 514
BspLI GGNNCC 6 cut(s) 57, 141, 369, 415, 502, 717
BspPI GGATC 2 cut(s) 710, 723
BspT107I GGYRCC 3 cut(s) 55, 367, 413
BspTNI GGTCTC 1 cut(s) 395
BsrI ACTGG 1 cut(s) 737
BssECI CCNNGG 5 cut(s) 211, 372, 417, 624, 780
BssMI GATC 4 cut(s) 360, 439, 660, 715
BssNI GRCGYC 5 cut(s) 187, 384, 414, 552, 606
BssT1I CCWWGG 3 cut(s) 211, 372, 624
Bst2UI CCWGG 1 cut(s) 461
Bst4CI ACNGT 9 cut(s) 24, 92, 106, 134, 139, 406, 572, 598, 790
Bst6I CTCTTC 1 cut(s) 182
BstACI GRCGYC 5 cut(s) 187, 384, 414, 552, 606
BstAPI GCANNNNNTGC 1 cut(s) 589
BstDEI CTNAG 2 cut(s) 486, 522
BstDSI CCRYGG 1 cut(s) 417
BstEII GGTNACC 1 cut(s) 784
BstH2I RGCGCY 1 cut(s) 417
BstHHI GCGC 2 cut(s) 416, 450
BstKTI GATC 4 cut(s) 363, 442, 663, 718
BstMAI GTCTC 3 cut(s) 325, 359, 395
BstMBI GATC 4 cut(s) 360, 439, 660, 715
BstMWI GCNNNNNNNGC 6 cut(s) 563, 589, 635, 644, 674, 770
BstNI CCWGG 1 cut(s) 461
BstPI GGTNACC 1 cut(s) 784
BstSCI CCNGG 2 cut(s) 459, 780
BstSLI GKGCMC 1 cut(s) 706
BstV1I GCAGC 3 cut(s) 569, 631, 706
BstV2I GAAGAC 3 cut(s) 159, 387, 400
BstX2I RGATCY 1 cut(s) 715
BstYI RGATCY 1 cut(s) 715
BsuRI GGCC 4 cut(s) 299, 629, 764, 773
BtgI CCRYGG 1 cut(s) 417
CfoI GCGC 2 cut(s) 416, 450
Cfr13I GGNCC 2 cut(s) 24, 501
CseI GACGC 4 cut(s) 176, 392, 455, 560
Csp6I GTAC 4 cut(s) 32, 56, 135, 284
CspCI CAANNNNNGTGG 4 cut(s) 135, 170, 652, 687
CviQI GTAC 4 cut(s) 32, 56, 135, 284
DdeI CTNAG 2 cut(s) 486, 522
DinI GGCGCC 1 cut(s) 415
DpnI GATC 4 cut(s) 362, 441, 662, 717
DpnII GATC 4 cut(s) 360, 439, 660, 715
DraIII CACNNNGTG 1 cut(s) 342
EaeI YGGCCR 2 cut(s) 297, 771
Eam1104I CTCTTC 1 cut(s) 182
EarI CTCTTC 1 cut(s) 182
Eco130I CCWWGG 3 cut(s) 211, 372, 624
Eco31I GGTCTC 1 cut(s) 395
Eco47I GGWCC 2 cut(s) 24, 501
Eco91I GGTNACC 1 cut(s) 784
EcoO109I RGGNCCY 1 cut(s) 501
EcoO65I GGTNACC 1 cut(s) 784
EcoRII CCWGG 1 cut(s) 459
EcoT14I CCWWGG 3 cut(s) 211, 372, 624
EgeI GGCGCC 1 cut(s) 415
EheI GGCGCC 1 cut(s) 415
ErhI CCWWGG 3 cut(s) 211, 372, 624
Esp3I CGTCTC 2 cut(s) 325, 359
FaiI YATR 3 cut(s) 36, 123, 770
FaqI GGGAC 1 cut(s) 453
FauI CCCGC 1 cut(s) 631
Fnu4HI GCNGC 8 cut(s) 300, 555, 558, 615, 645, 648, 695, 765
Fsp4HI GCNGC 8 cut(s) 300, 555, 558, 615, 645, 648, 695, 765
FspBI CTAG 1 cut(s) 212
GlaI GCGC 2 cut(s) 415, 449
GluI GCNGC 8 cut(s) 300, 555, 558, 615, 645, 648, 695, 765
HaeII RGCGCY 1 cut(s) 417
HaeIII GGCC 4 cut(s) 299, 629, 764, 773
HapII CCGG 2 cut(s) 546, 782
HgaI GACGC 4 cut(s) 176, 392, 455, 560
HhaI GCGC 2 cut(s) 416, 450
Hin1I GRCGYC 5 cut(s) 187, 384, 414, 552, 606
Hin6I GCGC 2 cut(s) 414, 448
HinP1I GCGC 2 cut(s) 414, 448
HincII GTYRAC 1 cut(s) 541
HindII GTYRAC 1 cut(s) 541
HindIII AAGCTT 1 cut(s) 494
HinfI GANTC 3 cut(s) 524, 530, 542
HpaII CCGG 2 cut(s) 546, 782
HphI GGTGA 4 cut(s) 389, 499, 560, 736
Hpy166II GTNNAC 1 cut(s) 541
Hpy188I TCNGA 2 cut(s) 529, 794
Hpy188III TCNNGA 1 cut(s) 358
Hpy8I GTNNAC 1 cut(s) 541
Hpy99I CGWCG 3 cut(s) 611, 635, 692
HpyAV CCTTC 5 cut(s) 301, 481, 514, 520, 754
HpyCH4III ACNGT 9 cut(s) 24, 92, 106, 134, 139, 406, 572, 598, 790
HpyCH4IV ACGT 1 cut(s) 606
HpyF10VI GCNNNNNNNGC 6 cut(s) 563, 589, 635, 644, 674, 770
HpyF3I CTNAG 2 cut(s) 486, 522
HpySE526I ACGT 1 cut(s) 606
Hsp92I GRCGYC 5 cut(s) 187, 384, 414, 552, 606
HspAI GCGC 2 cut(s) 414, 448
KasI GGCGCC 1 cut(s) 413
KpnI GGTACC 1 cut(s) 59
Kzo9I GATC 4 cut(s) 360, 439, 660, 715
LmnI GCTCC 4 cut(s) 274, 421, 463, 641
LpnPI CCDG 6 cut(s) 446, 473, 548, 559, 654, 718
Lsp1109I GCAGC 3 cut(s) 569, 631, 706
LweI GCATC 2 cut(s) 592, 601
MaeI CTAG 1 cut(s) 212
MaeII ACGT 1 cut(s) 606
MaeIII GTNAC 5 cut(s) 334, 517, 548, 742, 784
MalI GATC 4 cut(s) 362, 441, 662, 717
MboI GATC 4 cut(s) 360, 439, 660, 715
MboII GAAGA 6 cut(s) 159, 169, 392, 400, 730, 769
MflI RGATCY 1 cut(s) 715
MhlI GDGCHC 1 cut(s) 706
MluCI AATT 2 cut(s) 66, 111
Mly113I GGCGCC 1 cut(s) 414
MlyI GAGTC 3 cut(s) 524, 533, 536
MmeI TCCRAC 1 cut(s) 43
MnlI CCTC 7 cut(s) 81, 137, 201, 204, 265, 360, 628
MseI TTAA 4 cut(s) 14, 110, 579, 711
MspI CCGG 2 cut(s) 546, 782
MspR9I CCNGG 2 cut(s) 461, 782
MvaI CCWGG 1 cut(s) 461
MwoI GCNNNNNNNGC 6 cut(s) 563, 589, 635, 644, 674, 770
NarI GGCGCC 1 cut(s) 414
NciI CCSGG 1 cut(s) 782
NdeII GATC 4 cut(s) 360, 439, 660, 715
NlaIV GGNNCC 6 cut(s) 57, 141, 369, 415, 502, 717
NmeAIII GCCGAG 2 cut(s) 163, 328
NmuCI GTSAC 4 cut(s) 334, 517, 548, 742
Pfl23II CGTACG 1 cut(s) 134
PflFI GACNNNGTC 1 cut(s) 523
PkrI GCNGC 8 cut(s) 301, 556, 559, 616, 646, 649, 696, 766
PleI GAGTC 3 cut(s) 524, 532, 536
PluTI GGCGCC 1 cut(s) 417
PpsI GAGTC 3 cut(s) 524, 532, 536
PpuMI RGGWCCY 1 cut(s) 501
Psp5II RGGWCCY 1 cut(s) 501
Psp6I CCWGG 1 cut(s) 459
PspEI GGTNACC 1 cut(s) 784
PspGI CCWGG 1 cut(s) 459
PspLI CGTACG 1 cut(s) 134
PspN4I GGNNCC 6 cut(s) 57, 141, 369, 415, 502, 717
PspPI GGNCC 2 cut(s) 24, 501
PspPPI RGGWCCY 1 cut(s) 501
PsuI RGATCY 1 cut(s) 715
PsyI GACNNNGTC 1 cut(s) 523
RsaI GTAC 4 cut(s) 33, 57, 136, 285
RsaNI GTAC 4 cut(s) 32, 56, 135, 284
SaqAI TTAA 4 cut(s) 14, 110, 579, 711
SatI GCNGC 8 cut(s) 300, 555, 558, 615, 645, 648, 695, 765
Sau3AI GATC 4 cut(s) 360, 439, 660, 715
Sau96I GGNCC 2 cut(s) 24, 501
SchI GAGTC 3 cut(s) 524, 533, 536
ScrFI CCNGG 2 cut(s) 461, 782
SduI GDGCHC 1 cut(s) 706
SfaNI GCATC 2 cut(s) 592, 601
SfiI GGCCNNNNNGGCC 1 cut(s) 770
SfoI GGCGCC 1 cut(s) 415
SinI GGWCC 2 cut(s) 24, 501
SmlI CTYRAG 4 cut(s) 425, 443, 491, 752
SmoI CTYRAG 4 cut(s) 425, 443, 491, 752
Sse9I AATT 2 cut(s) 66, 111
SsiI CCGC 8 cut(s) 300, 555, 602, 614, 638, 647, 650, 765
SspDI GGCGCC 1 cut(s) 413
SspMI CTAG 1 cut(s) 212
StyD4I CCNGG 2 cut(s) 459, 780
StyI CCWWGG 3 cut(s) 211, 372, 624
TaaI ACNGT 9 cut(s) 24, 92, 106, 134, 139, 406, 572, 598, 790
TaiI ACGT 1 cut(s) 609
TaqI TCGA 4 cut(s) 359, 533, 609, 633
TasI AATT 2 cut(s) 66, 111
TatI WGTACW 1 cut(s) 31
TauI GCSGC 5 cut(s) 302, 557, 617, 650, 767
Tru1I TTAA 4 cut(s) 14, 110, 579, 711
Tru9I TTAA 4 cut(s) 14, 110, 579, 711
TseFI GTSAC 4 cut(s) 334, 517, 548, 742
TseI GCWGC 3 cut(s) 557, 644, 694
Tsp45I GTSAC 4 cut(s) 334, 517, 548, 742
TspDTI ATGAA 1 cut(s) 463
TspGWI ACGGA 2 cut(s) 153, 353
Tth111I GACNNNGTC 1 cut(s) 523
VpaK11BI GGWCC 2 cut(s) 24, 501
XmaJI CCTAGG 1 cut(s) 211
XspI CTAG 1 cut(s) 212
ZraI GACGTC 1 cut(s) 607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.