Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0007932

Physical Location & Seq

Genomic Coordinates Forward (+)

18432261 .. 18439272

Gene Structure 7012 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4632 bp

ATGGGTCCCACTCTCCTCCTCCTTCTCCATGGAAGCACCTCCTCAGCTCCTTCGTTATCTCCAGCAACTCAGCAATGGTCGATTACCCTTTTAACAGCTCTGCAGGCTTTGCCTGTTTTGGAGCTGGCTTCAATTCTCATCAATTTGGTATTCTTTGTTGGGTTCCTCTTCGTCCTCTCAGCTCGACAGGCGTTTGCTTGTCTGGGTAGGATTAGAATCTTCAAGGACAATTCGGGTTCGAACTCGAGCTCAATTCGGCATAATAGTGCTGTGGATGGAGGAACTCATGAGATTAGAGTTGGCAGAGACTTCAAATTTTCCGTCTTTTGTTGTTTCTATGTGTTGTTTGTACAAGTTTTGGTGTTGGGTTTTGATGGGGTTGGTCTGATAAGGGAGAGACGTAATGGGAATGTTGTGGATTGGTCTGTGATGGTCTTGCCTGCTGCACAAGCTTCGGCTTGGTTCGCGCTGAGCTTTTCGGCGCTGCATTGTAAATTCAAGGTGTGTGAGAAATTCCCACTGCTTTTGAGGGTCTGGTGGTCAGTGTCATTTCTGATTTGCTTGTGCACTTTATATGTTGATGGGAGAGCTTTTGCAATTGAAGGATCGAAACGCATGAGTTCTCATGTTGTGGCAAATCTTGCTGTGACACCGGCTCTAGCGTTTCTTTGTTTCGTCGCGTTTAGGGGTGTTACTGGTATTCAAGTTTCTGGACAATCGGATCTTCAAGAGCCATTGCTGGAAGAAGAAGCAGGGTGTCTTAAGGTTAGCCCTTATCATGATGCTGGGCTTTTCAGTTTAGCCACACTGTCTTGGATGAACCCGCTTCTTTCAATTGGGGCAAAGAGACCGCTTGAGATCAAGGACATTCCCCTTCTTGCGCCACAAGATCGAGCCAAAACCAATTATAAGATCTTGAATTCAAATTGGGAGAAAGGTAAGGCTGAAAACCCTTCAGGGCAGCCTTCTTTAGCCTGGGCAATTCTCAAGTCCTTTTGGAAGGAGGCAGCTTGTAATGCTGTATTTGCTGGATTGAATACTCTTGTTTCATATGTGGGTCCATTTATGATTAGCTATTTTGTTGATTACTTAGGGGGGATAGAGACGTTCCCCCATGAAGGGTATATCCTTGCTGGTACTTTCTTTGCGGCAAAGCTTGTTGAGACCTTAACGACCCGGCAGTGGTACCTCGGGGTAGACATTTTGGGTATGCATGTAAGATCAGCTCTAACGGCAATGGTATACCGAAAAGGACTTAGACTTTCCAGCTTAGCCAAGCAAAGTCACACTAGTGGAGAGATTGTTAACTACATGGCTGTTGATGTGCAAAGGATAGGGGACTACTCTTGGTATCTCCATGACATGTGGATGCTTCCTATGCAAATCATTCTTGCCCTCGCAATTTTGTACAAGAATGTTGGAATTGCTTCTGTTGCGACATTGATTGCCACCATTATCTCCATTGTTCTTACTGTTCCAGTGGCAAAGATACAAGAAGAGTATCAAGACAAGTTGATGACTGCCAAGGATGAAAGAATGAGGAAGACTTCTGAGTGTCTGCGAAACATGAGGATTCTCAAGTTGCAAGCTTGGGAGGACAGGTACCGGTTGAAGTTAGAGGAAATGCGCGGTGTAGAGTTCAAGTGGCTGCGCAAAGCTCTCTACTCTCAAGCTTTTATTACATTCATGTTCTGGAGCTCTCCTATATTTGTTTCAGCCGTAACATTTGGTACCTCTATATTCTTGGGTCACCATCTAACTGCAGGTGGTGTTCTTTCTGCTCTAGCCACTTTCAGGATACTTCAAGAACCACTCAGGAATTTCCCTGACCTTGTATCAATGATGGCTCAGACAAAAGTTTCCCTTGATCGAATTTCTGGATTCCTGCAGGAGGAAGAGTTGCAGGAGGATGCAACAATTGTCCTACCAGTAGGCATCACAACTACATCAATAGAAATTGAAGATGGTGTCTTTTGCTGGGACCCTTCATCACCCAGGCCAACGTTATCAGGAATACAAATGAAAGTGGAAAAAGGGATGCGTGTGGCTGTTTGTGGAATGGTTGGTGCTGGAAAGTCAAGCTTTCTCTCTTGCATCCTTGGTGAGATTCCGAAAATCTCCGGTGAAGTAAGGCTATGTGGCACTGCTGCTTATGTTCCTCAGTCAGCGTGGATACAGTCTGGAAATATTGAAGAAAATATTCTTTTTGGCAGCCCAATGGATAAACCAAAGTACAAGAAGGTTATCCATGCTTGTTCACTGAAAAAGGATTTGGAACTTTTCTCACATGGAGATCAGACCATAATTGGTGATAGGGGTATAAATCTGAGTGGTGGCCAGAAGCAACGTGTGCAGCTCGCAAGGGCGCTGTATCAAGATGCTGACATTTATTTACTTGATGACCCCTTTAGTGCAGTTGATGCACACACTGGGTCAGAGTTGTTTAAGGAATACATATTGACAGCATTAGAAGATAAAACTGTGGTCTTTGTGACCCATCAAGTTGAATTTCTGCCTGCTGCTGATTTAATACTGGTTCTTAAAGGAGGCCGCATCATACAGGCAGGAAAATATGATGATCTTTTACAGGCAGGAACTGATTTTAAATCACTAGTCTCAGCTCACCATGAAGCAATTGAAGCTATGGATATTCCCAATTATTCATCGGGAGATTCAGATCAAAGCTTGTGTGCAGATATTGGGCTCCGAAAAAATTGTGATAAACCTAGCAGCAGTGTTGACTGTTTGGCAAAGGAAGTGCAAGAAGGTATGTCAGCTTCAGAGCAGAAAGCAATTAAAGAGAAAAAGAAAGCAAAGCGCTCAAGAAAAAAGCAGCTTGTCCAAGAAGAGGAAAGGGTACGAGGTAGAGTCAGCATGAAGGTCTACTTTTCATATATGGCTGCAGCATATAAAGGATTGCTGATTCCACTCATAATCATTGCACAAATAGTATTTCAGTTCCTTCAAATCGCTAGTAGTTGGTGGATGGCTTGGGCAAATCCCCAAACAGAAGGAGACCAACCGAAAGTGAGTTCGATGGTCCTCCTTGTTGTTTACATGGCCCTTGCATTCGGAAGCTCTTGGTTTATATTTGTTAGGGCTGTTTTGGTAGCTACATTTGGTCTAGCAGCTGCTCAAAAATTGTTTGTGAAGATGCTTAGAAGTGTGTTCCGGGCACCAATGTCTTTCTTTGACTCTACTCCTGCTGGACGGATCTTGAATCGTGTATCAATTGATCAAAGTGTGGTGGATCTTGACATTCCTTTTAGACTTGGTGGGTTTGCTTCAACGACAATACAACTTATTGGTATTGTTGGTGTGATGACAACAGTTACCTGGCAAGTTTTGCTGCTTGTTATTCCTATGGCCATTGCTTGTTTGTGGATGCAGAAATACTACATGTCTTCATCAAGGGAACTGGTCCGTATTGTTAGCATCCAGAAATCTCCAATTATCCATCTTTTTGGTGAGTCAATTGCTGGAGCTGCCACTATAAGAGGATTTGGACAAGAAAAAAGGTTCATGAAGAGGAATCTTTATCTTCTTGATTGCTTTGCTCGCCCATTCTTCTGCAGTATTGCAGCTATTGAATGGCTCTGTCTGCGCATGGAATTACTCTCAACTTTTGTATTCGCTTTCTGCATGATTTTACTTGTGAGTTTTCCACATGGGAATAAAAGATCGATCCAAGTAAGCATGGCTGGCCTCGCTGTCACATATGGTCTGAATTTAAATGCACGCCTGTCACGATGGATACTTAGCTTTTGCAAGCTTGAAAACAAAATTATTTCCATTGAAAGGATATATCAGTACAGTCAAATCCCAAGTGAAGCTCCATCCCTTATTGAGGATTCTCAACCTCCATCTACATGGCCAGAGAATGGAACAATTGACATAATTGATTTAAAGGTTCGCTACAAGGAAAATCTTCCTGTTGTCCTTCATGGGGTAACCTGTAGTTTTCCCGGTGGGAAGAAGATTGGAATTGTTGGGCGTACTGGAAGTGGTAAATCTACCTTGATCCAGGCATTATTTCGACTTATTGAACCATCAGCTGGGAGGATCCTTATAGACAATGTTGATATTTCGACGATTGGTCTTCATGATCTCCGAAGCCGTCTCAGTATCATACCCCAGGATCCTACCTTATTTGAAGGGACCATTAGGCTCAATCTTGATCCCCTTGAAGAGCAGATGGATCATGAGATTTGGCAGGCACTTGACAAATCTCAGCTTGGAGACGTAATCCGTGAAAAAGAGCAAAAGCTTGATGCACCAGTGCTGGAAAATGGAGATAATTGGAGTGTGGGGCAGCGGCAACTTGTTTCTCTCGGAAGAGCATTGCTTAAGCAGGCAAAAATATTGGTGCTTGATGAAGCGACAGCATCAGTTGATACAGCCACAGACAATCTCATCCAGAAGATTATTCGTACAGAGTTTAAGAACTGCACCGTGTGCACTATCGCACATCGTATTCCAACCGTCATCGACAGTGATCTAGTATTGGTACTCAGTGATGGTCGAGTTGCAGAGTTCGATACTCCTACACGACTATTAGAAGATAAGTCATCCATGTTTCTAAAATTGGTAACAGAATATTCCTCTAGGTCAAGTGGCATACCAGAGTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0004888 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006810 GO:0006873 GO:0006875 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008281 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009987 GO:0010033 GO:0010118 GO:0015399 GO:0015405 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019725 GO:0022804 GO:0022857 GO:0023052 GO:0030003 GO:0030004 GO:0030007 GO:0031090 GO:0032870 GO:0033993 GO:0038023 GO:0042221 GO:0042592 GO:0042623 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055065 GO:0055067 GO:0055075 GO:0055080 GO:0055082 GO:0055085 GO:0060089 GO:0065007 GO:0065008 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0097305 GO:0097306 GO:0098588 GO:0098771 GO:0098805 GO:1901527 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ABC_membrane ABC_tran

Protein Analysis

1544

Amino Acids

171.41

Weight (kDa)

8.44

Isoelectric Point (pI)

43.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ABC_membrane	PF00664	340 - 605	6.1e-29	ABC transporter transmembrane region
ABC_tran	PF00005	669 - 803	1.2e-18	ABC transporter
ABC_membrane	PF00664	977 - 1231	1.1e-30	ABC transporter transmembrane region
ABC_tran	PF00005	1314 - 1462	1.9e-27	ABC transporter

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0007932)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04120 AT1G04120 AT1G04120
fragaria_vesca	FvH4_2g20350 FvH4_2g20350 FvH4_2g20350
malus_domestica	MD05G1187600.v1.1 MD10G1175300.v1.1
prunus_persica	Prupe.8G214600_v2.0.a1 Prupe.8G214600_v2.0.a1 Prupe.8G214600_v2.0.a1
pyrus_communis	pycom05g17240 pycom10g15100
rosa_chinensis	RchiOBHm_Chr6g0286471
rosa_laevigata	RLG00000012569
rosa_multiflora	Rmu_sc0034370.1_g000001
rosa_roxburghii	Rroxscaffold_7G00182310
rosa_rugosa	Rorug06G0179900 Rorug06G0180000
rosa_samantha	Rh6AG292200 Rh6BG295800 Rh6CG296500 Rh6DG288300
rosa_wichuraiana	Rw6G025200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	909
AarI	CACCTGC	1 cut(s)	1754
Acc16I	TGCGCA	2 cut(s)	1652, 3605
Acc36I	ACCTGC	1 cut(s)	1754
Acc65I	GGTACC	3 cut(s)	1185, 1602, 1730
AccB1I	GGYRCC	4 cut(s)	1185, 1602, 1730, 3175
AccB7I	CCANNNNNTGG	2 cut(s)	3881, 4055
AccI	GTMKAC	3 cut(s)	1197, 1242, 2883
AccII	CGCG	3 cut(s)	467, 680, 1629
AciI	CCGC	6 cut(s)	824, 851, 1148, 1629, 2551, 4315
AclI	AACGTT	1 cut(s)	2003
AcoI	YGGCCR	3 cut(s)	2335, 3366, 3872
AcsI	RAATTY	8 cut(s)	314, 494, 512, 919, 1819, 1872, 2507, 3727
AcuI	CTGAAG	2 cut(s)	939, 2763
AdeI	CACNNNGTG	1 cut(s)	4455
AfeI	AGCGCT	1 cut(s)	2819
AflII	CTTAAG	2 cut(s)	761, 4346
AflIII	ACRYGT	3 cut(s)	1362, 2347, 3399
AgeI	ACCGGT	1 cut(s)	1605
AhdI	GACNNNNNGTC	1 cut(s)	4095
AhlI	ACTAGT	2 cut(s)	1289, 2611
AjnI	CCWGG	5 cut(s)	974, 1994, 3335, 4023, 4134
AjuI	GAANNNNNNNTTGG	2 cut(s)	2255, 2287
AleI	CACNNNNGTG	1 cut(s)	1290
Alw21I	GWGCWC	4 cut(s)	251, 569, 1700, 4460
Alw26I	GTCTC	9 cut(s)	300, 391, 841, 1097, 1157, 2620, 3009, 4124, 4233
Alw44I	GTGCAC	2 cut(s)	565, 4456
AlwNI	CAGNNNCTG	1 cut(s)	2597
Ama87I	CYCGRG	2 cut(s)	244, 1190
Aor51HI	AGCGCT	1 cut(s)	2819
AoxI	GGCC	7 cut(s)	1997, 2335, 2548, 3060, 3366, 3703, 3872
ApaLI	GTGCAC	2 cut(s)	565, 4456
ApoI	RAATTY	8 cut(s)	314, 494, 512, 919, 1819, 1872, 2507, 3727
ArsI	GACNNNNNNTTYG	2 cut(s)	306, 338
AsiGI	ACCGGT	1 cut(s)	1605
Asp700I	GAANNNNTTC	3 cut(s)	1426, 2199, 3927
Asp718I	GGTACC	3 cut(s)	1185, 1602, 1730
AspLEI	GCGC	8 cut(s)	469, 484, 883, 1629, 1653, 2368, 2820, 3606
AspS9I	GGNCC	7 cut(s)	5, 1058, 1981, 3040, 3061, 3421, 4158
AsuC2I	CCSGG	3 cut(s)	1177, 3173, 3966
AsuHPI	GGTGA	7 cut(s)	1742, 1983, 2114, 2135, 2321, 2615, 3479
AsuII	TTCGAA	1 cut(s)	239
AvaI	CYCGRG	2 cut(s)	244, 1190
AvaII	GGWCC	6 cut(s)	5, 1058, 1981, 3040, 3421, 4158
BaeGI	GKGCMC	3 cut(s)	569, 3178, 4460
BalI	TGGCCA	3 cut(s)	2337, 3368, 3874
BamHI	GGATCC	2 cut(s)	4062, 4138
BanI	GGYRCC	4 cut(s)	1185, 1602, 1730, 3175
BanII	GRGCYC	3 cut(s)	251, 1700, 2706
BarI	GAAGNNNNNNTAC	2 cut(s)	2761, 2793
BbsI	GAAGAC	3 cut(s)	1550, 3396, 4091
Bbv12I	GWGCWC	4 cut(s)	251, 569, 1700, 4460
BbvCI	CCTCAGC	1 cut(s)	43
BceAI	ACGGC	3 cut(s)	1248, 1703, 4101
BcgI	CGANNNNNNTGC	2 cut(s)	435, 469
BciT130I	CCWGG	5 cut(s)	976, 1996, 3337, 4025, 4136
BciVI	GTATCC	3 cut(s)	1791, 2166, 3747
BclI	TGATCA	1 cut(s)	3235
BcnI	CCSGG	3 cut(s)	1177, 3173, 3966
BcoDI	GTCTC	9 cut(s)	300, 391, 841, 1097, 1157, 2620, 3009, 4124, 4233
BcuI	ACTAGT	2 cut(s)	1289, 2611
BfaI	CTAG	9 cut(s)	659, 1290, 1784, 2612, 2727, 2973, 3125, 4499, 4605
BfmI	CTRYAG	6 cut(s)	101, 1761, 1886, 2901, 3571, 3955
BfoI	RGCGCY	3 cut(s)	485, 2369, 2821
BfrI	CTTAAG	2 cut(s)	761, 4346
BfuAI	ACCTGC	1 cut(s)	1754
BfuI	GTATCC	3 cut(s)	1791, 2166, 3747
BglII	AGATCT	1 cut(s)	912
BlpI	GCTNAGC	2 cut(s)	470, 1270
Bme1390I	CCNGG	8 cut(s)	976, 1177, 1996, 3173, 3337, 3966, 4025, 4136
Bme18I	GGWCC	6 cut(s)	5, 1058, 1981, 3040, 3421, 4158
BmeRI	GACNNNNNGTC	1 cut(s)	4095
BmeT110I	CYCGRG	2 cut(s)	244, 1190
BmgT120I	GGNCC	7 cut(s)	5, 1058, 1981, 3040, 3061, 3421, 4158
BmrFI	CCNGG	8 cut(s)	976, 1177, 1996, 3173, 3337, 3966, 4025, 4136
BmrI	ACTGGG	1 cut(s)	2439
BmuI	ACTGGG	1 cut(s)	2439
BpiI	GAAGAC	3 cut(s)	1550, 3396, 4091
BpmI	CTGGAG	3 cut(s)	45, 1714, 3501
Bpu10I	CCTNAGC	1 cut(s)	43
Bpu1102I	GCTNAGC	2 cut(s)	470, 1270
Bpu14I	TTCGAA	1 cut(s)	239
BpuEI	CTTGAG	5 cut(s)	875, 971, 1562, 1653, 2806
BpuMI	CCSGG	3 cut(s)	1177, 3173, 3966
Bsa29I	ATCGAT	1 cut(s)	3683
BsaBI	GATNNNNATC	2 cut(s)	215, 2676
BsaI	GGTCTC	3 cut(s)	841, 1157, 3009
BsaJI	CCNNGG	7 cut(s)	28, 975, 1189, 1524, 1994, 2098, 4134
BsaWI	WCCGGW	2 cut(s)	1605, 2120
BsaXI	ACNNNNNCTCC	6 cut(s)	1287, 1317, 1586, 1616, 1883, 1913
Bse118I	RCCGGY	2 cut(s)	652, 1605
Bse1I	ACTGG	8 cut(s)	700, 1478, 1928, 2434, 2538, 3423, 4003, 4277
Bse3DI	GCAATG	6 cut(s)	80, 734, 1242, 2937, 3369, 4340
Bse8I	GATNNNNATC	2 cut(s)	215, 2676
BseBI	CCWGG	5 cut(s)	976, 1996, 3337, 4025, 4136
BseCI	ATCGAT	1 cut(s)	3683
BseDI	CCNNGG	7 cut(s)	28, 975, 1189, 1524, 1994, 2098, 4134
BseJI	GATNNNNATC	2 cut(s)	215, 2676
BseMI	GCAATG	6 cut(s)	80, 734, 1242, 2937, 3369, 4340
BseNI	ACTGG	8 cut(s)	700, 1478, 1928, 2434, 2538, 3423, 4003, 4277
BseRI	GAGGAG	3 cut(s)	5, 8, 31
BseSI	GKGCMC	3 cut(s)	569, 3178, 4460
BseYI	CCCAGC	3 cut(s)	785, 1977, 4055
BsgI	GTGCAG	5 cut(s)	429, 2372, 2433, 2712, 4432
Bsh1236I	CGCG	3 cut(s)	467, 680, 1629
BshFI	GGCC	7 cut(s)	1999, 2337, 2550, 3062, 3368, 3705, 3874
BshNI	GGYRCC	4 cut(s)	1185, 1602, 1730, 3175
BshTI	ACCGGT	1 cut(s)	1605
BshVI	ATCGAT	1 cut(s)	3683
BsiHKAI	GWGCWC	4 cut(s)	251, 569, 1700, 4460
BsiHKCI	CYCGRG	2 cut(s)	244, 1190
BsiSI	CCGG	6 cut(s)	653, 1177, 1606, 2121, 3172, 3966
BslFI	GGGAC	3 cut(s)	1352, 1994, 4171
BsmAI	GTCTC	9 cut(s)	300, 391, 841, 1097, 1157, 2620, 3009, 4124, 4233
BsmBI	CGTCTC	4 cut(s)	391, 1097, 4124, 4233
BsmFI	GGGAC	3 cut(s)	1352, 1994, 4171
BsmI	GAATGC	1 cut(s)	3068
BsnI	GGCC	7 cut(s)	1999, 2337, 2550, 3062, 3368, 3705, 3874
Bso31I	GGTCTC	3 cut(s)	841, 1157, 3009
BsoBI	CYCGRG	2 cut(s)	244, 1190
Bsp119I	TTCGAA	1 cut(s)	239
Bsp1286I	GDGCHC	6 cut(s)	251, 569, 1700, 2706, 3178, 4460
Bsp1407I	TGTACA	2 cut(s)	349, 1407
Bsp1720I	GCTNAGC	2 cut(s)	470, 1270
Bsp19I	CCATGG	1 cut(s)	28
BspACI	CCGC	6 cut(s)	824, 851, 1148, 1629, 2551, 4315
BspANI	GGCC	7 cut(s)	1999, 2337, 2550, 3062, 3368, 3705, 3874
BspDI	ATCGAT	1 cut(s)	3683
BspFNI	CGCG	3 cut(s)	467, 680, 1629
BspHI	TCATGA	5 cut(s)	286, 778, 3522, 4102, 4201
BspMAI	CTGCAG	5 cut(s)	105, 1765, 1890, 2905, 3575
BspMI	ACCTGC	1 cut(s)	1754
BspQI	GCTCTTC	2 cut(s)	4182, 4330
BspT104I	TTCGAA	1 cut(s)	239
BspT107I	GGYRCC	4 cut(s)	1185, 1602, 1730, 3175
BspTI	CTTAAG	2 cut(s)	761, 4346
BspTNI	GGTCTC	3 cut(s)	841, 1157, 3009
BsrDI	GCAATG	6 cut(s)	80, 734, 1242, 2937, 3369, 4340
BsrFI	RCCGGY	2 cut(s)	652, 1605
BsrGI	TGTACA	2 cut(s)	349, 1407
BsrI	ACTGG	8 cut(s)	700, 1478, 1928, 2434, 2538, 3423, 4003, 4277
BssAI	RCCGGY	2 cut(s)	652, 1605
BssECI	CCNNGG	7 cut(s)	28, 975, 1189, 1524, 1994, 2098, 4134
BssNAI	GTATAC	1 cut(s)	1243
BssT1I	CCWWGG	3 cut(s)	28, 1524, 2098
Bst1107I	GTATAC	1 cut(s)	1243
Bst2UI	CCWGG	5 cut(s)	976, 1996, 3337, 4025, 4136
Bst6I	CTCTTC	7 cut(s)	173, 1491, 1890, 2841, 3521, 4182, 4330
BstAFI	CTTAAG	2 cut(s)	761, 4346
BstAPI	GCANNNNNTGC	6 cut(s)	109, 641, 2350, 2420, 3346, 4455
BstAUI	TGTACA	2 cut(s)	349, 1407
BstBI	TTCGAA	1 cut(s)	239
BstDSI	CCRYGG	1 cut(s)	28
BstEII	GGTNACC	2 cut(s)	1748, 3949
BstENI	CCTNNNNNAGG	2 cut(s)	1889, 3845
BstFNI	CGCG	3 cut(s)	467, 680, 1629
BstH2I	RGCGCY	3 cut(s)	485, 2369, 2821
BstHHI	GCGC	8 cut(s)	469, 484, 883, 1629, 1653, 2368, 2820, 3606
BstMAI	GTCTC	9 cut(s)	300, 391, 841, 1097, 1157, 2620, 3009, 4124, 4233
BstNI	CCWGG	5 cut(s)	976, 1996, 3337, 4025, 4136
BstNSI	RCATGY	3 cut(s)	1217, 1366, 3403
BstPI	GGTNACC	2 cut(s)	1748, 3949
BstSCI	CCNGG	8 cut(s)	974, 1175, 1994, 3171, 3335, 3964, 4023, 4134
BstSFI	CTRYAG	6 cut(s)	101, 1761, 1886, 2901, 3571, 3955
BstSLI	GKGCMC	3 cut(s)	569, 3178, 4460
BstUI	CGCG	3 cut(s)	467, 680, 1629
BstV2I	GAAGAC	3 cut(s)	1550, 3396, 4091
BstX2I	RGATCY	6 cut(s)	721, 912, 3213, 3250, 4062, 4138
BstXI	CCANNNNNNTGG	2 cut(s)	3464, 3870
BstYI	RGATCY	6 cut(s)	721, 912, 3213, 3250, 4062, 4138
BstZ17I	GTATAC	1 cut(s)	1243
Bsu15I	ATCGAT	1 cut(s)	3683
BsuI	GTATCC	3 cut(s)	1791, 2166, 3747
BsuRI	GGCC	7 cut(s)	1999, 2337, 2550, 3062, 3368, 3705, 3874
BsuTUI	ATCGAT	1 cut(s)	3683
BtgI	CCRYGG	1 cut(s)	28
BtsI	GCAGTG	4 cut(s)	518, 1187, 2142, 2740
BveI	ACCTGC	1 cut(s)	1754
CaiI	CAGNNNCTG	1 cut(s)	2597
CciI	TCATGA	5 cut(s)	286, 778, 3522, 4102, 4201
CfoI	GCGC	8 cut(s)	469, 484, 883, 1629, 1653, 2368, 2820, 3606
Cfr10I	RCCGGY	2 cut(s)	652, 1605
Cfr13I	GGNCC	7 cut(s)	5, 1058, 1981, 3040, 3061, 3421, 4158
ClaI	ATCGAT	1 cut(s)	3683
CspAI	ACCGGT	1 cut(s)	1605
CspCI	CAANNNNNGTGG	2 cut(s)	507, 542
DraI	TTTAAA	3 cut(s)	2605, 3732, 3906
DraIII	CACNNNGTG	1 cut(s)	4455
DriI	GACNNNNNGTC	1 cut(s)	4095
EaeI	YGGCCR	3 cut(s)	2335, 3366, 3872
Eam1104I	CTCTTC	7 cut(s)	173, 1491, 1890, 2841, 3521, 4182, 4330
Eam1105I	GACNNNNNGTC	1 cut(s)	4095
EarI	CTCTTC	7 cut(s)	173, 1491, 1890, 2841, 3521, 4182, 4330
Ecl136II	GAGCTC	2 cut(s)	249, 1698
Eco130I	CCWWGG	3 cut(s)	28, 1524, 2098
Eco24I	GRGCYC	3 cut(s)	251, 1700, 2706
Eco31I	GGTCTC	3 cut(s)	841, 1157, 3009
Eco47I	GGWCC	6 cut(s)	5, 1058, 1981, 3040, 3421, 4158
Eco47III	AGCGCT	1 cut(s)	2819
Eco53kI	GAGCTC	2 cut(s)	249, 1698
Eco57I	CTGAAG	2 cut(s)	939, 2763
Eco88I	CYCGRG	2 cut(s)	244, 1190
Eco91I	GGTNACC	2 cut(s)	1748, 3949
EcoICRI	GAGCTC	2 cut(s)	249, 1698
EcoNI	CCTNNNNNAGG	2 cut(s)	1889, 3845
EcoO109I	RGGNCCY	2 cut(s)	5, 1981
EcoO65I	GGTNACC	2 cut(s)	1748, 3949
EcoRI	GAATTC	1 cut(s)	919
EcoRII	CCWGG	5 cut(s)	974, 1994, 3335, 4023, 4134
EcoT14I	CCWWGG	3 cut(s)	28, 1524, 2098
EcoT22I	ATGCAT	1 cut(s)	1215
EcoT38I	GRGCYC	3 cut(s)	251, 1700, 2706
ErhI	CCWWGG	3 cut(s)	28, 1524, 2098
Esp3I	CGTCTC	4 cut(s)	391, 1097, 4124, 4233
FalI	AAGNNNNNCTT	6 cut(s)	1848, 1880, 2066, 2098, 2870, 2902
FaqI	GGGAC	3 cut(s)	1352, 1994, 4171
FauI	CCCGC	1 cut(s)	831
FauNDI	CATATG	2 cut(s)	1051, 3718
FbaI	TGATCA	1 cut(s)	3235
FblI	GTMKAC	3 cut(s)	1197, 1242, 2883
FriOI	GRGCYC	3 cut(s)	251, 1700, 2706
FspBI	CTAG	9 cut(s)	659, 1290, 1784, 2612, 2727, 2973, 3125, 4499, 4605
FspI	TGCGCA	2 cut(s)	1652, 3605
GlaI	GCGC	8 cut(s)	468, 483, 882, 1628, 1652, 2367, 2819, 3605
GsaI	CCCAGC	3 cut(s)	789, 1981, 4059
GsuI	CTGGAG	3 cut(s)	45, 1714, 3501
HaeII	RGCGCY	3 cut(s)	485, 2369, 2821
HaeIII	GGCC	7 cut(s)	1999, 2337, 2550, 3062, 3368, 3705, 3874
HapII	CCGG	6 cut(s)	653, 1177, 1606, 2121, 3172, 3966
HhaI	GCGC	8 cut(s)	469, 484, 883, 1629, 1653, 2368, 2820, 3606
Hin6I	GCGC	8 cut(s)	467, 482, 881, 1627, 1651, 2366, 2818, 3604
HinP1I	GCGC	8 cut(s)	467, 482, 881, 1627, 1651, 2366, 2818, 3604
HincII	GTYRAC	2 cut(s)	1306, 2740
HindII	GTYRAC	2 cut(s)	1306, 2740
HindIII	AAGCTT	8 cut(s)	450, 1154, 1587, 1671, 2080, 2683, 3770, 4265
HpaI	GTTAAC	1 cut(s)	1306
HpaII	CCGG	6 cut(s)	653, 1177, 1606, 2121, 3172, 3966
HphI	GGTGA	7 cut(s)	1742, 1983, 2114, 2135, 2321, 2615, 3479
Hpy166II	GTNNAC	9 cut(s)	567, 1198, 1243, 1306, 2258, 2740, 2884, 3055, 4458
Hpy8I	GTNNAC	9 cut(s)	567, 1198, 1243, 1306, 2258, 2740, 2884, 3055, 4458
Hpy99I	CGWCG	2 cut(s)	680, 4093
HpyCH4IV	ACGT	5 cut(s)	400, 1106, 2003, 2347, 4242
HpySE526I	ACGT	5 cut(s)	400, 1106, 2003, 2347, 4242
HspAI	GCGC	8 cut(s)	467, 482, 881, 1627, 1651, 2366, 2818, 3604
KflI	GGGWCCC	2 cut(s)	5, 1981
KpnI	GGTACC	3 cut(s)	1189, 1606, 1734
Ksp22I	TGATCA	1 cut(s)	3235
KspAI	GTTAAC	1 cut(s)	1306
LguI	GCTCTTC	2 cut(s)	4182, 4330
LmnI	GCTCC	6 cut(s)	52, 121, 1695, 2709, 3482, 3838
MaeI	CTAG	9 cut(s)	659, 1290, 1784, 2612, 2727, 2973, 3125, 4499, 4605
MaeII	ACGT	5 cut(s)	400, 1106, 2003, 2347, 4242
MfeI	CAATTG	7 cut(s)	597, 834, 1917, 2634, 3231, 3474, 3888
MflI	RGATCY	6 cut(s)	721, 912, 3213, 3250, 4062, 4138
MhlI	GDGCHC	6 cut(s)	251, 569, 1700, 2706, 3178, 4460
MlsI	TGGCCA	3 cut(s)	2337, 3368, 3874
MluNI	TGGCCA	3 cut(s)	2337, 3368, 3874
MlyI	GAGTC	3 cut(s)	2877, 3188, 3479
MmeI	TCCRAC	2 cut(s)	1399, 4502
Mox20I	TGGCCA	3 cut(s)	2337, 3368, 3874
Mph1103I	ATGCAT	1 cut(s)	1215
MroXI	GAANNNNTTC	3 cut(s)	1426, 2199, 3927
MscI	TGGCCA	3 cut(s)	2337, 3368, 3874
MslI	CAYNNNNRTG	4 cut(s)	264, 1290, 1367, 3868
Msp20I	TGGCCA	3 cut(s)	2337, 3368, 3874
MspA1I	CMGCKG	3 cut(s)	3131, 4055, 4315
MspCI	CTTAAG	2 cut(s)	761, 4346
MspI	CCGG	6 cut(s)	653, 1177, 1606, 2121, 3172, 3966
MspR9I	CCNGG	8 cut(s)	976, 1177, 1996, 3173, 3337, 3966, 4025, 4136
MunI	CAATTG	7 cut(s)	597, 834, 1917, 2634, 3231, 3474, 3888
Mva1269I	GAATGC	1 cut(s)	3068
MvaI	CCWGG	5 cut(s)	976, 1996, 3337, 4025, 4136
MvnI	CGCG	3 cut(s)	467, 680, 1629
NciI	CCSGG	3 cut(s)	1177, 3173, 3966
NcoI	CCATGG	1 cut(s)	28
NdeI	CATATG	2 cut(s)	1051, 3718
NmuCI	GTSAC	6 cut(s)	646, 1283, 1748, 2491, 3712, 3744
NsbI	TGCGCA	2 cut(s)	1652, 3605
NsiI	ATGCAT	1 cut(s)	1215
NspI	RCATGY	3 cut(s)	1217, 1366, 3403
NspV	TTCGAA	1 cut(s)	239
OliI	CACNNNNGTG	1 cut(s)	1290
PaeR7I	CTCGAG	1 cut(s)	244
PagI	TCATGA	5 cut(s)	286, 778, 3522, 4102, 4201
PaqCI	CACCTGC	1 cut(s)	1754
PciI	ACATGT	2 cut(s)	1362, 3399
PciSI	GCTCTTC	2 cut(s)	4182, 4330
PcsI	WCGNNNNNNNCGW	1 cut(s)	3745
PctI	GAATGC	1 cut(s)	3068
PdmI	GAANNNNTTC	3 cut(s)	1426, 2199, 3927
PfeI	GAWTC	9 cut(s)	216, 1573, 1881, 2107, 2672, 2923, 3220, 3532, 3851
PflMI	CCANNNNNTGG	2 cut(s)	3881, 4055
PinAI	ACCGGT	1 cut(s)	1605
PleI	GAGTC	3 cut(s)	2876, 3188, 3478
PpsI	GAGTC	3 cut(s)	2876, 3188, 3478
PpuMI	RGGWCCY	2 cut(s)	5, 1981
PscI	ACATGT	2 cut(s)	1362, 3399
PsiI	TTATAA	1 cut(s)	909
Psp124BI	GAGCTC	2 cut(s)	251, 1700
Psp1406I	AACGTT	1 cut(s)	2003
Psp5II	RGGWCCY	2 cut(s)	5, 1981
Psp6I	CCWGG	5 cut(s)	974, 1994, 3335, 4023, 4134
PspEI	GGTNACC	2 cut(s)	1748, 3949
PspFI	CCCAGC	3 cut(s)	785, 1977, 4055
PspGI	CCWGG	5 cut(s)	974, 1994, 3335, 4023, 4134
PspPI	GGNCC	7 cut(s)	5, 1058, 1981, 3040, 3061, 3421, 4158
PspPPI	RGGWCCY	2 cut(s)	5, 1981
PspXI	VCTCGAGB	1 cut(s)	244
PstI	CTGCAG	5 cut(s)	105, 1765, 1890, 2905, 3575
PstNI	CAGNNNCTG	1 cut(s)	2597
PsuI	RGATCY	6 cut(s)	721, 912, 3213, 3250, 4062, 4138
PvuII	CAGCTG	2 cut(s)	3131, 4055
RseI	CAYNNNNRTG	4 cut(s)	264, 1290, 1367, 3868
SacI	GAGCTC	2 cut(s)	251, 1700
SapI	GCTCTTC	2 cut(s)	4182, 4330
Sau96I	GGNCC	7 cut(s)	5, 1058, 1981, 3040, 3061, 3421, 4158
SbfI	CCTGCAGG	1 cut(s)	1890
SchI	GAGTC	3 cut(s)	2877, 3188, 3479
ScrFI	CCNGG	8 cut(s)	976, 1177, 1996, 3173, 3337, 3966, 4025, 4136
SdaI	CCTGCAGG	1 cut(s)	1890
SduI	GDGCHC	6 cut(s)	251, 569, 1700, 2706, 3178, 4460
SfcI	CTRYAG	6 cut(s)	101, 1761, 1886, 2901, 3571, 3955
Sfr274I	CTCGAG	1 cut(s)	244
SfuI	TTCGAA	1 cut(s)	239
SinI	GGWCC	6 cut(s)	5, 1058, 1981, 3040, 3421, 4158
SlaI	CTCGAG	1 cut(s)	244
SmiI	ATTTAAAT	1 cut(s)	3732
SmiMI	CAYNNNNRTG	4 cut(s)	264, 1290, 1367, 3868
SmlI	CTYRAG	8 cut(s)	244, 761, 854, 986, 1577, 1668, 2821, 4346
SmoI	CTYRAG	8 cut(s)	244, 761, 854, 986, 1577, 1668, 2821, 4346
SpeI	ACTAGT	2 cut(s)	1289, 2611
Sse8387I	CCTGCAGG	1 cut(s)	1890
SsiI	CCGC	6 cut(s)	824, 851, 1148, 1629, 2551, 4315
SspI	AATATT	4 cut(s)	2188, 2200, 4362, 4598
SspMI	CTAG	9 cut(s)	659, 1290, 1784, 2612, 2727, 2973, 3125, 4499, 4605
SstI	GAGCTC	2 cut(s)	251, 1700
StyD4I	CCNGG	8 cut(s)	974, 1175, 1994, 3171, 3335, 3964, 4023, 4134
StyI	CCWWGG	3 cut(s)	28, 1524, 2098
SwaI	ATTTAAAT	1 cut(s)	3732
TaiI	ACGT	5 cut(s)	403, 1109, 2006, 2350, 4245
TatI	WGTACW	4 cut(s)	349, 1407, 2232, 3810
TauI	GCSGC	3 cut(s)	1151, 2553, 4318
TfiI	GAWTC	9 cut(s)	216, 1573, 1881, 2107, 2672, 2923, 3220, 3532, 3851
TseFI	GTSAC	6 cut(s)	646, 1283, 1748, 2491, 3712, 3744
Tsp45I	GTSAC	6 cut(s)	646, 1283, 1748, 2491, 3712, 3744
TspGWI	ACGGA	4 cut(s)	310, 3226, 3413, 4238
Van91I	CCANNNNNTGG	2 cut(s)	3881, 4055
Vha464I	CTTAAG	2 cut(s)	761, 4346
VneI	GTGCAC	2 cut(s)	565, 4456
VpaK11BI	GGWCC	6 cut(s)	5, 1058, 1981, 3040, 3421, 4158
XagI	CCTNNNNNAGG	2 cut(s)	1889, 3845
XapI	RAATTY	8 cut(s)	314, 494, 512, 919, 1819, 1872, 2507, 3727
XceI	RCATGY	3 cut(s)	1217, 1366, 3403
XhoI	CTCGAG	1 cut(s)	244
XmiI	GTMKAC	3 cut(s)	1197, 1242, 2883
XmnI	GAANNNNTTC	3 cut(s)	1426, 2199, 3927
XspI	CTAG	9 cut(s)	659, 1290, 1784, 2612, 2727, 2973, 3125, 4499, 4605
Zsp2I	ATGCAT	1 cut(s)	1215

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.