pycom10g16910

Bystin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0008571
Physical Location & Seq
Reverse (-)
20115484 .. 20118418
2935 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1443 bp
ATGGTTAGGGTTTTAGACTGCAGAAATCAAGGTGGCGGGGGTTTAAACGTTCCAGTTTCAATAGCAGAAAAGGCTTCGCCTGCAGCTGCAGCAGCATCAATGGGGAAGAAGAGGCAGAGGGAGAGGCACCAGAATCCGGAGCCGTTCCTCGCTGAAGAAAATGGCTCCGTCGCCTCCAAGAAGCGGACCAAGTCGAAGCAGCACCAGCAGGAGGAGAAGCTCATATCTTCCGGAATGAGCTCCAAGATCCTCAAGCAGGCTCTGGCTCAGCAGAGGGAGATTGAGGACGAGGAGAACCGCGCCCAAAACCCTAATAATTCGTTGCTGGATGTATCGGAAGAGCCGCGGAAGGAGGAGGCCGACGGCGAAGAGGAAGACATCGATGATTTTGGTGGATTCTCCGAAACGCAGAGCCGTTTTGACGGTTATGAAGAGAGTGATGAACATGGTGGGAAGCTATTAAATGAGATTGATGAACATGATGAGAAGATATTGGAGCAATTCTTGTCAAAGGATGCCGGTCCGCAACGAACATTGGCCGACCTCATTGTTGCAAGAATCAAAGAGAAAGATTCAAATGTCGGCTCAGATGCGAGGCCTCTGCCTAAATTGGATACCTCCGTCATCGATTTGTATAAGGGGGTTGGTAAGTTTCTTAATAAGTACACTGCAGGGAAGATTCCCAAGGCATTCAAACACATCCCTTCAGTGCCACGATGGGAGGATGTCCTATATTTGACTGAGCCTGAGAATTGGTCACCAAATGCAATGTATCAGGCTACGAGAATTTTTGCTTCCAATTTGGGTAAGGAGAAGGTAGAACGTTTCTACAAGCTTGTGTTGCTCCCAAGGGTGAGAGAAGACATCCGCAAGCATAAGCGCTTGCATTTTGCACTATATCAATCTTTGAAGAAGTCTTTGTACAAGCCTGCTGCCTTTTTTATAGGAATACTGTTTCCCTTGTGTGAGTCAGGGACATGTAATCTAAGAGAAGCTGTTATTTTTGGGAGCATTATTCAGAAGGTCTCCATTCCTCCACTTCATTCTAGTGTTGCTTTAATGAAGCTGGCAGAAATGAAGTACTGTGGCACAACGAGTTATTTTATAAAGCTTCTTCTGGATAAAAAGTATGCCTTGCCATATCGCGTACTGGACGCTCTTGTTGCACATTTTATGAGATTTATGGAGGAAACAAGAGTAATGCCTGTGATATGGCACCAGTCACTTCTTACATTTGTGCAAAGGTACAAAAATGAATTGCAAAAGGAGGACAAAGATAATCTCAGAAATCTTCTTGAAACGCAAAAACACTATTTGGTAACCCCTGAAATAAGAAGGGAACTAGATAACAGCCGCAATCGTGGTGAGAAAGAGGACGATCGATCATGTCAATTGATATCCTTTGTTCATTTTTGCTTTGAATTTTCATTTGTTTCACATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bystin

Protein Analysis

481

Amino Acids

55.06

Weight (kDa)

7.64

Isoelectric Point (pI)

47.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bystin PF05291 175 - 457 2.8e-130 Bystin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1106
AccB1I GGYRCC 2 cut(s) 126, 1215
AccII CGCG 3 cut(s) 300, 346, 1146
AccIII TCCGGA 2 cut(s) 136, 230
AciI CCGC 8 cut(s) 36, 184, 298, 344, 346, 524, 868, 1354
AclI AACGTT 2 cut(s) 48, 823
AclWI GGATC 1 cut(s) 241
AcoI YGGCCR 1 cut(s) 537
AcsI RAATTY 2 cut(s) 786, 1421
AcuI CTGAAG 2 cut(s) 174, 690
AfaI GTAC 5 cut(s) 665, 923, 1082, 1149, 1247
AfeI AGCGCT 1 cut(s) 881
AfiI CCNNNNNNNGG 4 cut(s) 136, 183, 211, 256
AflIII ACRYGT 1 cut(s) 977
AgsI TTSAA 6 cut(s) 60, 576, 694, 910, 1298, 1421
AluBI AGCT 8 cut(s) 86, 220, 240, 457, 835, 995, 1066, 1111
AluI AGCT 8 cut(s) 86, 220, 240, 457, 835, 995, 1066, 1111
Alw21I GWGCWC 1 cut(s) 242
Alw26I GTCTC 1 cut(s) 1030
AlwI GGATC 1 cut(s) 241
AlwNI CAGNNNCTG 1 cut(s) 262
Aor13HI TCCGGA 2 cut(s) 136, 230
Aor51HI AGCGCT 1 cut(s) 881
AoxI GGCC 3 cut(s) 357, 537, 596
ApeKI GCWGC 6 cut(s) 83, 86, 89, 92, 199, 932
ApoI RAATTY 2 cut(s) 786, 1421
AspLEI GCGC 2 cut(s) 302, 882
AspS9I GGNCC 2 cut(s) 186, 521
AsuHPI GGTGA 3 cut(s) 750, 865, 1376
AvaII GGWCC 2 cut(s) 186, 521
BaeI ACNNNNGTAYC 2 cut(s) 606, 639
BanI GGYRCC 2 cut(s) 126, 1215
BanII GRGCYC 1 cut(s) 242
BarI GAAGNNNNNNTAC 2 cut(s) 905, 937
BbsI GAAGAC 2 cut(s) 381, 867
Bbv12I GWGCWC 1 cut(s) 242
BbvI GCAGC 6 cut(s) 73, 95, 101, 104, 211, 919
BccI CCATC 1 cut(s) 711
BceAI ACGGC 3 cut(s) 127, 379, 399
BcgI CGANNNNNNTGC 2 cut(s) 583, 617
BciVI GTATCC 1 cut(s) 607
BcoDI GTCTC 1 cut(s) 1030
BfaI CTAG 2 cut(s) 1047, 1343
BfmI CTRYAG 4 cut(s) 19, 81, 87, 669
BfoI RGCGCY 1 cut(s) 883
BfuI GTATCC 1 cut(s) 607
BisI GCNGC 8 cut(s) 84, 87, 90, 93, 200, 344, 933, 1354
BlpI GCTNAGC 1 cut(s) 267
BlsI GCNGC 8 cut(s) 85, 88, 91, 94, 201, 345, 934, 1355
BmcAI AGTACT 1 cut(s) 1082
Bme18I GGWCC 2 cut(s) 186, 521
BmgT120I GGNCC 2 cut(s) 186, 521
BmiI GGNNCC 4 cut(s) 128, 141, 166, 1217
BmsI GCATC 3 cut(s) 104, 505, 580
BpiI GAAGAC 2 cut(s) 381, 867
Bpu1102I GCTNAGC 1 cut(s) 267
BpuEI CTTGAG 1 cut(s) 236
Bsa29I ATCGAT 3 cut(s) 381, 627, 1381
BsaI GGTCTC 1 cut(s) 1030
BsaJI CCNNGG 3 cut(s) 344, 684, 848
BsaWI WCCGGW 2 cut(s) 136, 230
Bsc4I CCNNNNNNNGG 4 cut(s) 136, 183, 211, 256
Bse118I RCCGGY 1 cut(s) 518
Bse1I ACTGG 3 cut(s) 53, 1155, 1219
Bse3DI GCAATG 1 cut(s) 774
BseAI TCCGGA 2 cut(s) 136, 230
BseCI ATCGAT 3 cut(s) 381, 627, 1381
BseDI CCNNGG 3 cut(s) 344, 684, 848
BseGI GGATG 5 cut(s) 334, 520, 699, 730, 864
BseLI CCNNNNNNNGG 4 cut(s) 136, 183, 211, 256
BseMI GCAATG 1 cut(s) 774
BseMII CTCAG 5 cut(s) 281, 600, 732, 738, 1297
BseNI ACTGG 3 cut(s) 53, 1155, 1219
BseRI GAGGAG 3 cut(s) 227, 305, 368
BseXI GCAGC 6 cut(s) 73, 95, 101, 104, 211, 919
Bsh1236I CGCG 3 cut(s) 300, 346, 1146
Bsh1285I CGRYCG 1 cut(s) 1381
BshFI GGCC 3 cut(s) 359, 539, 598
BshNI GGYRCC 2 cut(s) 126, 1215
BshVI ATCGAT 3 cut(s) 381, 627, 1381
BsiEI CGRYCG 1 cut(s) 1381
BsiHKAI GWGCWC 1 cut(s) 242
BsiSI CCGG 3 cut(s) 137, 231, 519
BslFI GGGAC 1 cut(s) 988
BslI CCNNNNNNNGG 4 cut(s) 136, 183, 211, 256
BsmAI GTCTC 1 cut(s) 1030
BsmFI GGGAC 1 cut(s) 988
BsmI GAATGC 1 cut(s) 689
BsnI GGCC 3 cut(s) 359, 539, 598
Bso31I GGTCTC 1 cut(s) 1030
Bsp1286I GDGCHC 1 cut(s) 242
Bsp13I TCCGGA 2 cut(s) 136, 230
Bsp1407I TGTACA 1 cut(s) 921
Bsp143I GATC 3 cut(s) 246, 1378, 1382
Bsp1720I GCTNAGC 1 cut(s) 267
BspACI CCGC 8 cut(s) 36, 184, 298, 344, 346, 524, 868, 1354
BspANI GGCC 3 cut(s) 359, 539, 598
BspCNI CTCAG 5 cut(s) 280, 599, 733, 739, 1296
BspDI ATCGAT 3 cut(s) 381, 627, 1381
BspEI TCCGGA 2 cut(s) 136, 230
BspFNI CGCG 3 cut(s) 300, 346, 1146
BspLI GGNNCC 4 cut(s) 128, 141, 166, 1217
BspMAI CTGCAG 4 cut(s) 23, 85, 91, 673
BspPI GGATC 1 cut(s) 241
BspQI GCTCTTC 1 cut(s) 333
BspT107I GGYRCC 2 cut(s) 126, 1215
BspTNI GGTCTC 1 cut(s) 1030
BsrDI GCAATG 1 cut(s) 774
BsrFI RCCGGY 1 cut(s) 518
BsrGI TGTACA 1 cut(s) 921
BsrI ACTGG 3 cut(s) 53, 1155, 1219
BssAI RCCGGY 1 cut(s) 518
BssECI CCNNGG 3 cut(s) 344, 684, 848
BssMI GATC 3 cut(s) 246, 1378, 1382
BssT1I CCWWGG 2 cut(s) 684, 848
Bst4CI ACNGT 3 cut(s) 425, 954, 1085
Bst6I CTCTTC 4 cut(s) 104, 333, 363, 426
BstAUI TGTACA 1 cut(s) 921
BstC8I GCNNGC 6 cut(s) 81, 258, 872, 884, 930, 1068
BstDEI CTNAG 6 cut(s) 267, 586, 741, 747, 986, 1283
BstDSI CCRYGG 1 cut(s) 344
BstEII GGTNACC 2 cut(s) 756, 1318
BstENI CCTNNNNNAGG 1 cut(s) 254
BstF5I GGATG 5 cut(s) 334, 520, 699, 730, 864
BstFNI CGCG 3 cut(s) 300, 346, 1146
BstH2I RGCGCY 1 cut(s) 883
BstHHI GCGC 2 cut(s) 302, 882
BstKTI GATC 3 cut(s) 249, 1381, 1385
BstMAI GTCTC 1 cut(s) 1030
BstMBI GATC 3 cut(s) 246, 1378, 1382
BstMCI CGRYCG 1 cut(s) 1381
BstMWI GCNNNNNNNGC 7 cut(s) 71, 80, 89, 92, 205, 841, 1163
BstNSI RCATGY 1 cut(s) 981
BstPI GGTNACC 2 cut(s) 756, 1318
BstSFI CTRYAG 4 cut(s) 19, 81, 87, 669
BstUI CGCG 3 cut(s) 300, 346, 1146
BstV1I GCAGC 6 cut(s) 73, 95, 101, 104, 211, 919
BstV2I GAAGAC 2 cut(s) 381, 867
BstX2I RGATCY 1 cut(s) 246
BstYI RGATCY 1 cut(s) 246
Bsu15I ATCGAT 3 cut(s) 381, 627, 1381
BsuI GTATCC 1 cut(s) 607
BsuRI GGCC 3 cut(s) 359, 539, 598
BsuTUI ATCGAT 3 cut(s) 381, 627, 1381
BtgI CCRYGG 1 cut(s) 344
BtsCI GGATG 5 cut(s) 334, 520, 699, 730, 864
BtsI GCAGTG 1 cut(s) 666
BtsIMutI CAGTG 2 cut(s) 666, 714
Cac8I GCNNGC 6 cut(s) 81, 258, 872, 884, 930, 1068
CaiI CAGNNNCTG 1 cut(s) 262
CfoI GCGC 2 cut(s) 302, 882
Cfr10I RCCGGY 1 cut(s) 518
Cfr13I GGNCC 2 cut(s) 186, 521
Cfr42I CCGCGG 1 cut(s) 347
ClaI ATCGAT 3 cut(s) 381, 627, 1381
CpoI CGGWCCG 1 cut(s) 521
CseI GACGC 1 cut(s) 1163
Csp6I GTAC 5 cut(s) 664, 922, 1081, 1148, 1246
CspI CGGWCCG 1 cut(s) 521
CviAII CATG 4 cut(s) 446, 479, 978, 1386
CviQI GTAC 5 cut(s) 664, 922, 1081, 1148, 1246
DdeI CTNAG 6 cut(s) 267, 586, 741, 747, 986, 1283
DpnI GATC 3 cut(s) 248, 1380, 1384
DpnII GATC 3 cut(s) 246, 1378, 1382
DraI TTTAAA 1 cut(s) 45
EaeI YGGCCR 1 cut(s) 537
Eam1104I CTCTTC 4 cut(s) 104, 333, 363, 426
EarI CTCTTC 4 cut(s) 104, 333, 363, 426
Ecl136II GAGCTC 1 cut(s) 240
Eco130I CCWWGG 2 cut(s) 684, 848
Eco147I AGGCCT 1 cut(s) 598
Eco24I GRGCYC 1 cut(s) 242
Eco31I GGTCTC 1 cut(s) 1030
Eco32I GATATC 1 cut(s) 1398
Eco47I GGWCC 2 cut(s) 186, 521
Eco47III AGCGCT 1 cut(s) 881
Eco53kI GAGCTC 1 cut(s) 240
Eco57I CTGAAG 2 cut(s) 174, 690
Eco91I GGTNACC 2 cut(s) 756, 1318
EcoICRI GAGCTC 1 cut(s) 240
EcoNI CCTNNNNNAGG 1 cut(s) 254
EcoO65I GGTNACC 2 cut(s) 756, 1318
EcoRV GATATC 1 cut(s) 1398
EcoT14I CCWWGG 2 cut(s) 684, 848
EcoT38I GRGCYC 1 cut(s) 242
ErhI CCWWGG 2 cut(s) 684, 848
FaeI CATG 4 cut(s) 449, 482, 981, 1389
FalI AAGNNNNNCTT 2 cut(s) 1118, 1150
FaqI GGGAC 1 cut(s) 988
FatI CATG 4 cut(s) 445, 478, 977, 1385
FauI CCCGC 1 cut(s) 29
Fnu4HI GCNGC 8 cut(s) 84, 87, 90, 93, 200, 344, 933, 1354
FokI GGATG 5 cut(s) 341, 527, 686, 737, 851
FriOI GRGCYC 1 cut(s) 242
Fsp4HI GCNGC 8 cut(s) 84, 87, 90, 93, 200, 344, 933, 1354
FspBI CTAG 2 cut(s) 1047, 1343
GlaI GCGC 2 cut(s) 301, 881
GluI GCNGC 8 cut(s) 84, 87, 90, 93, 200, 344, 933, 1354
HaeII RGCGCY 1 cut(s) 883
HaeIII GGCC 3 cut(s) 359, 539, 598
HapII CCGG 3 cut(s) 137, 231, 519
HgaI GACGC 1 cut(s) 1163
HhaI GCGC 2 cut(s) 302, 882
Hin1II CATG 4 cut(s) 449, 482, 981, 1389
Hin6I GCGC 2 cut(s) 300, 880
HinP1I GCGC 2 cut(s) 300, 880
HindIII AAGCTT 2 cut(s) 833, 1109
HinfI GANTC 6 cut(s) 133, 396, 558, 572, 679, 968
HpaII CCGG 3 cut(s) 137, 231, 519
HphI GGTGA 3 cut(s) 750, 865, 1376
Hpy166II GTNNAC 1 cut(s) 666
Hpy188I TCNGA 5 cut(s) 337, 403, 589, 1020, 1286
Hpy188III TCNNGA 4 cut(s) 137, 231, 1118, 1295
Hpy8I GTNNAC 1 cut(s) 666
Hpy99I CGWCG 2 cut(s) 173, 365
HpyAV CCTTC 5 cut(s) 343, 714, 808, 1015, 1329
HpyCH4III ACNGT 3 cut(s) 425, 954, 1085
HpyCH4IV ACGT 2 cut(s) 48, 823
HpyF10VI GCNNNNNNNGC 7 cut(s) 71, 80, 89, 92, 205, 841, 1163
HpyF3I CTNAG 6 cut(s) 267, 586, 741, 747, 986, 1283
HpySE526I ACGT 2 cut(s) 48, 823
Hsp92II CATG 4 cut(s) 449, 482, 981, 1389
HspAI GCGC 2 cut(s) 300, 880
Kpn2I TCCGGA 2 cut(s) 136, 230
KspI CCGCGG 1 cut(s) 347
Kzo9I GATC 3 cut(s) 246, 1378, 1382
LguI GCTCTTC 1 cut(s) 333
LmnI GCTCC 6 cut(s) 139, 170, 245, 496, 849, 1008
Lsp1109I GCAGC 6 cut(s) 73, 95, 101, 104, 211, 919
LweI GCATC 3 cut(s) 104, 505, 580
MaeI CTAG 2 cut(s) 1047, 1343
MaeII ACGT 2 cut(s) 48, 823
MaeIII GTNAC 3 cut(s) 756, 1221, 1318
MalI GATC 3 cut(s) 248, 1380, 1384
MboI GATC 3 cut(s) 246, 1378, 1382
MfeI CAATTG 1 cut(s) 1391
MflI RGATCY 1 cut(s) 246
MhlI GDGCHC 1 cut(s) 242
MluCI AATT 9 cut(s) 316, 500, 608, 751, 786, 799, 1256, 1391, 1421
MlyI GAGTC 1 cut(s) 977
MroI TCCGGA 2 cut(s) 136, 230
MseI TTAA 4 cut(s) 44, 461, 657, 1058
MslI CAYNNNNRTG 1 cut(s) 1047
MspA1I CMGCKG 2 cut(s) 86, 346
MspI CCGG 3 cut(s) 137, 231, 519
MssI GTTTAAAC 1 cut(s) 45
MunI CAATTG 1 cut(s) 1391
Mva1269I GAATGC 1 cut(s) 689
MvnI CGCG 3 cut(s) 300, 346, 1146
MwoI GCNNNNNNNGC 7 cut(s) 71, 80, 89, 92, 205, 841, 1163
NdeII GATC 3 cut(s) 246, 1378, 1382
NlaIII CATG 4 cut(s) 449, 482, 981, 1389
NlaIV GGNNCC 4 cut(s) 128, 141, 166, 1217
NmuCI GTSAC 2 cut(s) 756, 1221
NspI RCATGY 1 cut(s) 981
PceI AGGCCT 1 cut(s) 598
PciI ACATGT 1 cut(s) 977
PciSI GCTCTTC 1 cut(s) 333
PctI GAATGC 1 cut(s) 689
PfeI GAWTC 5 cut(s) 133, 396, 558, 572, 679
PflFI GACNNNGTC 1 cut(s) 190
PkrI GCNGC 8 cut(s) 85, 88, 91, 94, 201, 345, 934, 1355
Ple19I CGATCG 1 cut(s) 1381
PleI GAGTC 1 cut(s) 976
PmeI GTTTAAAC 1 cut(s) 45
PpsI GAGTC 1 cut(s) 976
PscI ACATGT 1 cut(s) 977
PsiI TTATAA 1 cut(s) 1106
Psp124BI GAGCTC 1 cut(s) 242
Psp1406I AACGTT 2 cut(s) 48, 823
PspEI GGTNACC 2 cut(s) 756, 1318
PspN4I GGNNCC 4 cut(s) 128, 141, 166, 1217
PspPI GGNCC 2 cut(s) 186, 521
PstI CTGCAG 4 cut(s) 23, 85, 91, 673
PstNI CAGNNNCTG 1 cut(s) 262
PsuI RGATCY 1 cut(s) 246
PsyI GACNNNGTC 1 cut(s) 190
PvuI CGATCG 1 cut(s) 1381
PvuII CAGCTG 1 cut(s) 86
RsaI GTAC 5 cut(s) 665, 923, 1082, 1149, 1247
RsaNI GTAC 5 cut(s) 664, 922, 1081, 1148, 1246
RseI CAYNNNNRTG 1 cut(s) 1047
Rsr2I CGGWCCG 1 cut(s) 521
RsrII CGGWCCG 1 cut(s) 521
SacI GAGCTC 1 cut(s) 242
SacII CCGCGG 1 cut(s) 347
SapI GCTCTTC 1 cut(s) 333
SaqAI TTAA 4 cut(s) 44, 461, 657, 1058
SatI GCNGC 8 cut(s) 84, 87, 90, 93, 200, 344, 933, 1354
Sau3AI GATC 3 cut(s) 246, 1378, 1382
Sau96I GGNCC 2 cut(s) 186, 521
ScaI AGTACT 1 cut(s) 1082
SchI GAGTC 1 cut(s) 977
SduI GDGCHC 1 cut(s) 242
SfaNI GCATC 3 cut(s) 104, 505, 580
SfcI CTRYAG 4 cut(s) 19, 81, 87, 669
Sfr303I CCGCGG 1 cut(s) 347
SgrBI CCGCGG 1 cut(s) 347
SinI GGWCC 2 cut(s) 186, 521
SmiMI CAYNNNNRTG 1 cut(s) 1047
SmlI CTYRAG 1 cut(s) 251
SmoI CTYRAG 1 cut(s) 251
Sse9I AATT 9 cut(s) 316, 500, 608, 751, 786, 799, 1256, 1391, 1421
SseBI AGGCCT 1 cut(s) 598
SsiI CCGC 8 cut(s) 36, 184, 298, 344, 346, 524, 868, 1354
SspMI CTAG 2 cut(s) 1047, 1343
SstI GAGCTC 1 cut(s) 242
StuI AGGCCT 1 cut(s) 598
StyI CCWWGG 2 cut(s) 684, 848
TaaI ACNGT 3 cut(s) 425, 954, 1085
TaiI ACGT 2 cut(s) 51, 826
TaqI TCGA 4 cut(s) 194, 381, 627, 1381
TasI AATT 9 cut(s) 316, 500, 608, 751, 786, 799, 1256, 1391, 1421
TatI WGTACW 3 cut(s) 663, 921, 1080
TauI GCSGC 2 cut(s) 346, 1356
TfiI GAWTC 5 cut(s) 133, 396, 558, 572, 679
Tru1I TTAA 4 cut(s) 44, 461, 657, 1058
Tru9I TTAA 4 cut(s) 44, 461, 657, 1058
TscAI CASTG 2 cut(s) 673, 714
TseFI GTSAC 2 cut(s) 756, 1221
TseI GCWGC 6 cut(s) 83, 86, 89, 92, 199, 932
Tsp45I GTSAC 2 cut(s) 756, 1221
TspDTI ATGAA 9 cut(s) 444, 456, 489, 1031, 1076, 1091, 1269, 1397, 1416
TspGWI ACGGA 2 cut(s) 157, 610
TspRI CASTG 2 cut(s) 673, 714
Tth111I GACNNNGTC 1 cut(s) 190
VpaK11BI GGWCC 2 cut(s) 186, 521
XagI CCTNNNNNAGG 1 cut(s) 254
XapI RAATTY 2 cut(s) 786, 1421
XceI RCATGY 1 cut(s) 981
XspI CTAG 2 cut(s) 1047, 1343
ZrmI AGTACT 1 cut(s) 1082
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.