pycom10g17110
NAC Family

NAC domain-containing protein 21

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0011962
Physical Location & Seq
Reverse (-)
20278857 .. 20283922
5066 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 942 bp
ATGGTGGAGGCAAAGTTGCCACCAGGGTTCAGGTTCCATCCAAGAGACGAAGAGCTTGTGTGTGACTATTTGATGAAGAAGGTGGCTCAATCTGACTCAACCCTTATGATTGAAGTTGACCTCAACAAGTCTGAGCCATGGGATATCCCTGAAAGTGCATGTGTGGGAAGGAAAGAATGGTACTTCTACAGTCAGCGTGATCGTAAATATGCAACAGGGCTTCGAACAAACCGTGCAACTGCAACAGGGTATTGGAAGGCCACAGGAAAAGATAGGTCAATCCGTCGCAAAGGAATCCTTGTTGGGATGAGAAAAACCTTGGTCTTCTACCAGGGCCGAGCTCCCAAAGGCAGAAAATCCGATTGGGTCATGCATGAGTTTCGGCTTGAAGGTCCTCTTAGTCCTCCTAAAATCTCTCCTCTCAAGGAAGATTGGGTTTTGTGCAGAGTGCTTTATAAAAACCGAGAGATAGCTGCCAAACCCAGCATGGGAATTAGCTGTTACGATGACACGAGCACTTTAACTCTGCCTGCCTTGATGGACTCCTACATCAGTTTCGATGGCCAAACTCAGACTCAGACACACACAGAGACTCAACCCCAAGAGTATGAGCAAGTGCCCTGCTTCTCCATGTTCTCCCAGAACCAAACCAACCCAATTTTCACACACACTGACATCACCAATTCTCAACCAAACATACCCACCAAGCACAATAATAATACCAACACAACCACATTTGGAGGGTTGCCAAATGTGACCAATTTTCTTGACCCTTTTTCATGTGACAAAAAGGTGCTAAAAGCTATTTTGAGTCACCTTACCAAGATGGAAAGTACCACTTATCCTACTTTGGATGGCTCATCACCAAGCTTTGGGGAAGGAAGTACAGAAAACTACCTATCTGATGTCGGCATGCCCAACAATATTTGGAGCCACTACTGA

Protein Analysis

314

Amino Acids

35.66

Weight (kDa)

8.11

Isoelectric Point (pI)

46.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 7 - 129 3.9e-43 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011962)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G56010 AT1G56010 AT3G12977 AT3G12977
fragaria_vesca FvH4_3g16040
malus_domestica MD10G1198400.v1.1
prunus_persica Prupe.4G143600_v2.0.a1
pyrus_communis pycom05g19830 pycom10g17110
rosa_chinensis RchiOBHm_Chr5g0027011
rosa_laevigata RLG00000032977 RLG00000032981
rosa_multiflora Rmu_sc0000882.1_g000012
rosa_roxburghii Rroxscaffold_1G00052480
rosa_rugosa Rorug05G0096600
rosa_samantha Rh5AG190000 Rh5BG187600 Rh5CG207400
rosa_wichuraiana Rw5G017300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 456
AccB7I CCANNNNNTGG 1 cut(s) 872
AcoI YGGCCR 1 cut(s) 562
AfaI GTAC 3 cut(s) 182, 835, 886
AfiI CCNNNNNNNGG 2 cut(s) 488, 872
AgsI TTSAA 2 cut(s) 113, 389
AjnI CCWGG 2 cut(s) 22, 330
AjuI GAANNNNNNNTTGG 4 cut(s) 346, 378, 644, 676
AluBI AGCT 6 cut(s) 55, 341, 473, 498, 803, 870
AluI AGCT 6 cut(s) 55, 341, 473, 498, 803, 870
Alw21I GWGCWC 2 cut(s) 343, 518
Alw26I GTCTC 2 cut(s) 39, 584
AoxI GGCC 3 cut(s) 258, 334, 562
ApeKI GCWGC 1 cut(s) 473
AspS9I GGNCC 2 cut(s) 334, 392
AsuHPI GGTGA 3 cut(s) 670, 806, 855
AsuII TTCGAA 1 cut(s) 223
AvaII GGWCC 1 cut(s) 392
BaeGI GKGCMC 1 cut(s) 621
BalI TGGCCA 1 cut(s) 564
BanII GRGCYC 1 cut(s) 343
BauI CACGAG 1 cut(s) 511
BbsI GAAGAC 1 cut(s) 316
Bbv12I GWGCWC 2 cut(s) 343, 518
BbvI GCAGC 1 cut(s) 460
BccI CCATC 5 cut(s) 45, 532, 554, 820, 848
BcgI CGANNNNNNTGC 2 cut(s) 362, 396
BciT130I CCWGG 2 cut(s) 24, 332
BcoDI GTCTC 2 cut(s) 39, 584
BfmI CTRYAG 1 cut(s) 187
BisI GCNGC 1 cut(s) 474
BlsI GCNGC 1 cut(s) 475
Bme1390I CCNGG 2 cut(s) 24, 332
Bme18I GGWCC 1 cut(s) 392
BmgT120I GGNCC 2 cut(s) 334, 392
BmiI GGNNCC 2 cut(s) 35, 932
BmrFI CCNGG 2 cut(s) 24, 332
BpiI GAAGAC 1 cut(s) 316
Bpu14I TTCGAA 1 cut(s) 223
BpuEI CTTGAG 1 cut(s) 407
BsaJI CCNNGG 4 cut(s) 23, 137, 318, 331
BsaXI ACNNNNNCTCC 1 cut(s) 29
Bsc4I CCNNNNNNNGG 2 cut(s) 488, 872
BseBI CCWGG 2 cut(s) 24, 332
BseDI CCNNGG 4 cut(s) 23, 137, 318, 331
BseGI GGATG 3 cut(s) 37, 312, 859
BseLI CCNNNNNNNGG 2 cut(s) 488, 872
BseMII CTCAG 3 cut(s) 123, 584, 590
BseRI GAGGAG 1 cut(s) 408
BseSI GKGCMC 1 cut(s) 621
BseXI GCAGC 1 cut(s) 460
BseYI CCCAGC 1 cut(s) 482
BsgI GTGCAG 1 cut(s) 463
BshFI GGCC 3 cut(s) 260, 336, 564
BsiHKAI GWGCWC 2 cut(s) 343, 518
BslI CCNNNNNNNGG 2 cut(s) 488, 872
BsmAI GTCTC 2 cut(s) 39, 584
BsmBI CGTCTC 1 cut(s) 39
BsnI GGCC 3 cut(s) 260, 336, 564
Bsp119I TTCGAA 1 cut(s) 223
Bsp1286I GDGCHC 3 cut(s) 343, 518, 621
Bsp143I GATC 1 cut(s) 199
Bsp19I CCATGG 1 cut(s) 137
BspANI GGCC 3 cut(s) 260, 336, 564
BspCNI CTCAG 3 cut(s) 124, 583, 589
BspLI GGNNCC 2 cut(s) 35, 932
BspQI GCTCTTC 1 cut(s) 45
BspT104I TTCGAA 1 cut(s) 223
BssECI CCNNGG 4 cut(s) 23, 137, 318, 331
BssMI GATC 1 cut(s) 199
BssSI CACGAG 1 cut(s) 511
BssT1I CCWWGG 2 cut(s) 137, 318
Bst2BI CACGAG 1 cut(s) 511
Bst2UI CCWGG 2 cut(s) 24, 332
Bst4CI ACNGT 2 cut(s) 191, 233
Bst6I CTCTTC 1 cut(s) 45
BstBI TTCGAA 1 cut(s) 223
BstC8I GCNNGC 2 cut(s) 531, 914
BstDEI CTNAG 4 cut(s) 132, 398, 570, 576
BstDSI CCRYGG 1 cut(s) 137
BstF5I GGATG 3 cut(s) 37, 312, 859
BstKTI GATC 1 cut(s) 202
BstMAI GTCTC 2 cut(s) 39, 584
BstMBI GATC 1 cut(s) 199
BstNI CCWGG 2 cut(s) 24, 332
BstNSI RCATGY 2 cut(s) 162, 916
BstSCI CCNGG 2 cut(s) 22, 330
BstSFI CTRYAG 1 cut(s) 187
BstSLI GKGCMC 1 cut(s) 621
BstV1I GCAGC 1 cut(s) 460
BstV2I GAAGAC 1 cut(s) 316
BsuRI GGCC 3 cut(s) 260, 336, 564
BtgI CCRYGG 1 cut(s) 137
BtsCI GGATG 3 cut(s) 37, 312, 859
BtsIMutI CAGTG 1 cut(s) 669
Cac8I GCNNGC 2 cut(s) 531, 914
Cfr13I GGNCC 2 cut(s) 334, 392
Csp6I GTAC 3 cut(s) 181, 834, 885
CviAII CATG 8 cut(s) 138, 159, 370, 374, 487, 631, 780, 913
CviQI GTAC 3 cut(s) 181, 834, 885
DdeI CTNAG 4 cut(s) 132, 398, 570, 576
DpnI GATC 1 cut(s) 201
DpnII GATC 1 cut(s) 199
EaeI YGGCCR 1 cut(s) 562
Eam1104I CTCTTC 1 cut(s) 45
EarI CTCTTC 1 cut(s) 45
Ecl136II GAGCTC 1 cut(s) 341
Eco130I CCWWGG 2 cut(s) 137, 318
Eco24I GRGCYC 1 cut(s) 343
Eco32I GATATC 1 cut(s) 145
Eco47I GGWCC 1 cut(s) 392
Eco53kI GAGCTC 1 cut(s) 341
EcoICRI GAGCTC 1 cut(s) 341
EcoO109I RGGNCCY 1 cut(s) 392
EcoRII CCWGG 2 cut(s) 22, 330
EcoRV GATATC 1 cut(s) 145
EcoT14I CCWWGG 2 cut(s) 137, 318
EcoT22I ATGCAT 1 cut(s) 375
EcoT38I GRGCYC 1 cut(s) 343
ErhI CCWWGG 2 cut(s) 137, 318
Esp3I CGTCTC 1 cut(s) 39
FaeI CATG 8 cut(s) 141, 162, 373, 377, 490, 634, 783, 916
FalI AAGNNNNNCTT 6 cut(s) 282, 314, 381, 413, 823, 855
FatI CATG 8 cut(s) 137, 158, 369, 373, 486, 630, 779, 912
Fnu4HI GCNGC 1 cut(s) 474
FokI GGATG 3 cut(s) 24, 319, 866
FriOI GRGCYC 1 cut(s) 343
Fsp4HI GCNGC 1 cut(s) 474
GluI GCNGC 1 cut(s) 474
GsaI CCCAGC 1 cut(s) 486
HaeIII GGCC 3 cut(s) 260, 336, 564
Hin1II CATG 8 cut(s) 141, 162, 373, 377, 490, 634, 783, 916
HincII GTYRAC 1 cut(s) 118
HindII GTYRAC 1 cut(s) 118
HindIII AAGCTT 1 cut(s) 868
HinfI GANTC 6 cut(s) 95, 294, 542, 574, 592, 811
HphI GGTGA 3 cut(s) 670, 806, 855
Hpy166II GTNNAC 1 cut(s) 118
Hpy188I TCNGA 6 cut(s) 94, 133, 361, 573, 579, 904
Hpy188III TCNNGA 1 cut(s) 767
Hpy8I GTNNAC 1 cut(s) 118
Hpy99I CGWCG 1 cut(s) 288
HpyAV CCTTC 5 cut(s) 73, 162, 250, 383, 872
HpyCH4III ACNGT 2 cut(s) 191, 233
HpyCH4V TGCA 6 cut(s) 158, 212, 236, 242, 373, 444
HpyF3I CTNAG 4 cut(s) 132, 398, 570, 576
Hsp92II CATG 8 cut(s) 141, 162, 373, 377, 490, 634, 783, 916
Kzo9I GATC 1 cut(s) 199
LguI GCTCTTC 1 cut(s) 45
LmnI GCTCC 2 cut(s) 346, 930
Lsp1109I GCAGC 1 cut(s) 460
MaeIII GTNAC 5 cut(s) 62, 500, 754, 782, 812
MalI GATC 1 cut(s) 201
MboI GATC 1 cut(s) 199
MboII GAAGA 4 cut(s) 62, 88, 316, 440
MhlI GDGCHC 3 cut(s) 343, 518, 621
MlsI TGGCCA 1 cut(s) 564
MluCI AATT 4 cut(s) 492, 657, 682, 760
MluNI TGGCCA 1 cut(s) 564
MlyI GAGTC 5 cut(s) 89, 536, 568, 586, 820
MnlI CCTC 5 cut(s) 131, 405, 414, 429, 734
Mox20I TGGCCA 1 cut(s) 564
Mph1103I ATGCAT 1 cut(s) 375
MscI TGGCCA 1 cut(s) 564
MseI TTAA 1 cut(s) 521
Msp20I TGGCCA 1 cut(s) 564
MspR9I CCNGG 2 cut(s) 24, 332
MvaI CCWGG 2 cut(s) 24, 332
NcoI CCATGG 1 cut(s) 137
NdeII GATC 1 cut(s) 199
NlaIII CATG 8 cut(s) 141, 162, 373, 377, 490, 634, 783, 916
NlaIV GGNNCC 2 cut(s) 35, 932
NmeAIII GCCGAG 1 cut(s) 362
NmuCI GTSAC 4 cut(s) 62, 754, 782, 812
NsiI ATGCAT 1 cut(s) 375
NspI RCATGY 2 cut(s) 162, 916
NspV TTCGAA 1 cut(s) 223
PaeI GCATGC 1 cut(s) 916
PciSI GCTCTTC 1 cut(s) 45
PcsI WCGNNNNNNNCGW 1 cut(s) 229
PfeI GAWTC 1 cut(s) 294
PflMI CCANNNNNTGG 1 cut(s) 872
PkrI GCNGC 1 cut(s) 475
PleI GAGTC 5 cut(s) 89, 536, 568, 586, 819
PpsI GAGTC 5 cut(s) 89, 536, 568, 586, 819
PpuMI RGGWCCY 1 cut(s) 392
PsiI TTATAA 1 cut(s) 456
Psp124BI GAGCTC 1 cut(s) 343
Psp5II RGGWCCY 1 cut(s) 392
Psp6I CCWGG 2 cut(s) 22, 330
PspFI CCCAGC 1 cut(s) 482
PspGI CCWGG 2 cut(s) 22, 330
PspN4I GGNNCC 2 cut(s) 35, 932
PspPI GGNCC 2 cut(s) 334, 392
PspPPI RGGWCCY 1 cut(s) 392
RsaI GTAC 3 cut(s) 182, 835, 886
RsaNI GTAC 3 cut(s) 181, 834, 885
SacI GAGCTC 1 cut(s) 343
SapI GCTCTTC 1 cut(s) 45
SaqAI TTAA 1 cut(s) 521
SatI GCNGC 1 cut(s) 474
Sau3AI GATC 1 cut(s) 199
Sau96I GGNCC 2 cut(s) 334, 392
SchI GAGTC 5 cut(s) 89, 536, 568, 586, 820
ScrFI CCNGG 2 cut(s) 24, 332
SduI GDGCHC 3 cut(s) 343, 518, 621
SfcI CTRYAG 1 cut(s) 187
SfuI TTCGAA 1 cut(s) 223
SinI GGWCC 1 cut(s) 392
SmlI CTYRAG 1 cut(s) 422
SmoI CTYRAG 1 cut(s) 422
SphI GCATGC 1 cut(s) 916
Sse9I AATT 4 cut(s) 492, 657, 682, 760
SspI AATATT 1 cut(s) 925
SstI GAGCTC 1 cut(s) 343
StyD4I CCNGG 2 cut(s) 22, 330
StyI CCWWGG 2 cut(s) 137, 318
TaaI ACNGT 2 cut(s) 191, 233
TaqI TCGA 2 cut(s) 223, 558
TasI AATT 4 cut(s) 492, 657, 682, 760
TatI WGTACW 1 cut(s) 884
TfiI GAWTC 1 cut(s) 294
Tru1I TTAA 1 cut(s) 521
Tru9I TTAA 1 cut(s) 521
TscAI CASTG 1 cut(s) 676
TseFI GTSAC 4 cut(s) 62, 754, 782, 812
TseI GCWGC 1 cut(s) 473
Tsp45I GTSAC 4 cut(s) 62, 754, 782, 812
TspDTI ATGAA 2 cut(s) 89, 768
TspGWI ACGGA 1 cut(s) 272
TspRI CASTG 1 cut(s) 676
Van91I CCANNNNNTGG 1 cut(s) 872
VpaK11BI GGWCC 1 cut(s) 392
XceI RCATGY 2 cut(s) 162, 916
XcmI CCANNNNNNNNNTGG 1 cut(s) 484
Zsp2I ATGCAT 1 cut(s) 375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.