pycom10g17940

Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0013210
Physical Location & Seq
Reverse (-)
21085980 .. 21090499
4520 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 777 bp
ATGAACAAGTTGGGTAAAGGCCAACGCGATAAGCTCCAGCAGTTCGTCATGATAACTGGAGCGAGTGAAAAAGTGGCTCTGCAGGTTCTGAAGGAGAGTGATTGGCATCTTGAAGGAGCGTTTGATATCTTCTACACTCAGCCCCAGCTGCAAGCAGAGACTGATTCTAATACTGATTCTAATACCAATTCTAGACAACTGGAGGAATTATACAATACATATAAAGATCCAGATTCTGATATGATACTTGTTGATGGTATCAGCCTCCTTCTCAACGATCTTCAGGTGGATCCTCAAGATATAGTCATGTTAGTTGTTTCCTGGCACATGAAGGCAGCTACCATGTGTGAATTTTCCAAGGAGGAGTTCGTAGAGGGATTAGAACCACTAGGGATAGATTCTTTGGACAAGTTCCGTGAAAAGATACCATTTATGAGATCCGAGCTGAAAGATGAACAAAAGTTTCGAGAGATATATAATTTTGCTTTTGACTGGGCAAAGGAAAAGGGTCAGAAATCTTTGGCATTGGATACTGCTATTGGAATGTGGCAATTGGTGTTTGCTGAAAAGCAGTGGCCGTATGTAGAACATTGGTGTCAGTTCTTACCGGCTCGGCATAACAAGGCCATTTCGAGGGACACATGGTCTCAACTATTGGAGTTTGCAACGGCCGTGGATCCGTCACTGTCAAACTATGACGCGGAAGGTGCCTGGCCATATCTTATTGACGAATTTGTCGAGTACTTGAAGGAGAACGGAGCAATCCAAAAGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cullin_binding UBA_4

Protein Analysis

259

Amino Acids

29.86

Weight (kDa)

4.6

Isoelectric Point (pI)

29.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UBA_8 PF22566 8 - 45 4.2e-10 UBA-like domain
UBA_4 PF14555 10 - 48 1.6e-11 UBA-like domain
Cullin_binding PF03556 138 - 248 4.7e-37 Cullin binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013210)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12760
fragaria_vesca FvH4_3g14590 FvH4_3g14590
malus_domestica MD05G1234900.v1.1 MD10G1211100.v1.1
prunus_persica Prupe.4G131300_v2.0.a1
pyrus_communis pycom05g21180 pycom10g17940
rosa_chinensis RchiOBHm_Chr5g0024051
rosa_laevigata RLG00000032757
rosa_multiflora Rmu_sc0011761.1_g000007
rosa_roxburghii Rroxscaffold_1G00055190
rosa_rugosa Rorug05G0081200 Rorug05G0081200
rosa_samantha Rh5AG171600 Rh5CG183800 Rh5DG170400
rosa_wichuraiana Rw5G015530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 734
Acc36I ACCTGC 1 cut(s) 73
AccB1I GGYRCC 1 cut(s) 707
AccII CGCG 2 cut(s) 27, 701
AciI CCGC 1 cut(s) 701
AclWI GGATC 6 cut(s) 221, 284, 297, 432, 671, 684
AcoI YGGCCR 3 cut(s) 575, 669, 713
AcsI RAATTY 2 cut(s) 350, 731
AcuI CTGAAG 2 cut(s) 110, 266
AfaI GTAC 1 cut(s) 743
AfiI CCNNNNNNNGG 1 cut(s) 633
AgsI TTSAA 2 cut(s) 113, 748
AhdI GACNNNNNGTC 1 cut(s) 643
AjnI CCWGG 2 cut(s) 320, 710
AjuI GAANNNNNNNTTGG 2 cut(s) 350, 382
AluBI AGCT 4 cut(s) 34, 148, 338, 445
AluI AGCT 4 cut(s) 34, 148, 338, 445
Alw26I GTCTC 2 cut(s) 152, 651
AlwI GGATC 6 cut(s) 221, 284, 297, 432, 671, 684
AlwNI CAGNNNCTG 3 cut(s) 88, 161, 236
AoxI GGCC 5 cut(s) 19, 575, 624, 669, 713
ApeKI GCWGC 2 cut(s) 148, 335
ApoI RAATTY 2 cut(s) 350, 731
BalI TGGCCA 1 cut(s) 715
BamHI GGATCC 2 cut(s) 289, 676
BanI GGYRCC 1 cut(s) 707
BbvI GCAGC 2 cut(s) 135, 347
BccI CCATC 1 cut(s) 248
BceAI ACGGC 3 cut(s) 562, 656, 684
BciT130I CCWGG 2 cut(s) 322, 712
BciVI GTATCC 1 cut(s) 523
BcoDI GTCTC 2 cut(s) 152, 651
BfaI CTAG 2 cut(s) 192, 389
BfmI CTRYAG 1 cut(s) 80
BfuAI ACCTGC 1 cut(s) 73
BfuI GTATCC 1 cut(s) 523
BisI GCNGC 2 cut(s) 149, 336
BlsI GCNGC 2 cut(s) 150, 337
BmcAI AGTACT 1 cut(s) 743
Bme1390I CCNGG 2 cut(s) 322, 712
BmeRI GACNNNNNGTC 1 cut(s) 643
BmiI GGNNCC 3 cut(s) 291, 678, 709
BmrFI CCNGG 2 cut(s) 322, 712
BmrI ACTGGG 1 cut(s) 502
BmsI GCATC 1 cut(s) 115
BmuI ACTGGG 1 cut(s) 502
BpmI CTGGAG 3 cut(s) 20, 78, 221
BpuEI CTTGAG 1 cut(s) 279
BsaBI GATNNNNATC 1 cut(s) 105
BsaI GGTCTC 1 cut(s) 651
BsaJI CCNNGG 2 cut(s) 357, 672
Bsc4I CCNNNNNNNGG 1 cut(s) 633
Bse118I RCCGGY 1 cut(s) 607
Bse1I ACTGG 3 cut(s) 61, 204, 497
Bse8I GATNNNNATC 1 cut(s) 105
BseBI CCWGG 2 cut(s) 322, 712
BseDI CCNNGG 2 cut(s) 357, 672
BseJI GATNNNNATC 1 cut(s) 105
BseLI CCNNNNNNNGG 1 cut(s) 633
BseMII CTCAG 1 cut(s) 152
BseNI ACTGG 3 cut(s) 61, 204, 497
BseRI GAGGAG 1 cut(s) 377
BseX3I CGGCCG 1 cut(s) 669
BseXI GCAGC 2 cut(s) 135, 347
BseYI CCCAGC 1 cut(s) 144
Bsh1236I CGCG 2 cut(s) 27, 701
Bsh1285I CGRYCG 1 cut(s) 672
BshFI GGCC 5 cut(s) 21, 577, 626, 671, 715
BshNI GGYRCC 1 cut(s) 707
BsiEI CGRYCG 1 cut(s) 672
BsiSI CCGG 1 cut(s) 608
BslFI GGGAC 1 cut(s) 650
BslI CCNNNNNNNGG 1 cut(s) 633
BsmAI GTCTC 2 cut(s) 152, 651
BsmFI GGGAC 1 cut(s) 650
BsnI GGCC 5 cut(s) 21, 577, 626, 671, 715
Bso31I GGTCTC 1 cut(s) 651
Bsp143I GATC 5 cut(s) 226, 277, 289, 437, 676
BspACI CCGC 1 cut(s) 701
BspANI GGCC 5 cut(s) 21, 577, 626, 671, 715
BspCNI CTCAG 1 cut(s) 151
BspFNI CGCG 2 cut(s) 27, 701
BspHI TCATGA 1 cut(s) 48
BspLI GGNNCC 3 cut(s) 291, 678, 709
BspMAI CTGCAG 1 cut(s) 84
BspMI ACCTGC 1 cut(s) 73
BspPI GGATC 6 cut(s) 221, 284, 297, 432, 671, 684
BspT107I GGYRCC 1 cut(s) 707
BspTNI GGTCTC 1 cut(s) 651
BsrFI RCCGGY 1 cut(s) 607
BsrI ACTGG 3 cut(s) 61, 204, 497
BssAI RCCGGY 1 cut(s) 607
BssECI CCNNGG 2 cut(s) 357, 672
BssMI GATC 5 cut(s) 226, 277, 289, 437, 676
BssT1I CCWWGG 1 cut(s) 357
Bst2UI CCWGG 2 cut(s) 322, 712
Bst4CI ACNGT 1 cut(s) 687
BstC8I GCNNGC 1 cut(s) 153
BstDEI CTNAG 1 cut(s) 138
BstDSI CCRYGG 1 cut(s) 672
BstFNI CGCG 2 cut(s) 27, 701
BstKTI GATC 5 cut(s) 229, 280, 292, 440, 679
BstMAI GTCTC 2 cut(s) 152, 651
BstMBI GATC 5 cut(s) 226, 277, 289, 437, 676
BstMCI CGRYCG 1 cut(s) 672
BstMWI GCNNNNNNNGC 2 cut(s) 148, 707
BstNI CCWGG 2 cut(s) 322, 712
BstSCI CCNGG 2 cut(s) 320, 710
BstSFI CTRYAG 1 cut(s) 80
BstUI CGCG 2 cut(s) 27, 701
BstV1I GCAGC 2 cut(s) 135, 347
BstX2I RGATCY 4 cut(s) 226, 289, 437, 676
BstYI RGATCY 4 cut(s) 226, 289, 437, 676
BstZI CGGCCG 1 cut(s) 669
BsuI GTATCC 1 cut(s) 523
BsuRI GGCC 5 cut(s) 21, 577, 626, 671, 715
BtgI CCRYGG 1 cut(s) 672
BtsI GCAGTG 1 cut(s) 578
BtsIMutI CAGTG 2 cut(s) 578, 683
BveI ACCTGC 1 cut(s) 73
Cac8I GCNNGC 1 cut(s) 153
CaiI CAGNNNCTG 3 cut(s) 88, 161, 236
CciI TCATGA 1 cut(s) 48
Cfr10I RCCGGY 1 cut(s) 607
CseI GACGC 1 cut(s) 707
Csp6I GTAC 1 cut(s) 742
CspCI CAANNNNNGTGG 2 cut(s) 654, 689
CviAII CATG 5 cut(s) 49, 307, 328, 343, 642
CviQI GTAC 1 cut(s) 742
DdeI CTNAG 1 cut(s) 138
DpnI GATC 5 cut(s) 228, 279, 291, 439, 678
DpnII GATC 5 cut(s) 226, 277, 289, 437, 676
DrdI GACNNNNNNGTC 1 cut(s) 734
DriI GACNNNNNGTC 1 cut(s) 643
DseDI GACNNNNNNGTC 1 cut(s) 734
EaeI YGGCCR 3 cut(s) 575, 669, 713
EagI CGGCCG 1 cut(s) 669
Eam1105I GACNNNNNGTC 1 cut(s) 643
EclXI CGGCCG 1 cut(s) 669
Eco130I CCWWGG 1 cut(s) 357
Eco31I GGTCTC 1 cut(s) 651
Eco32I GATATC 1 cut(s) 127
Eco52I CGGCCG 1 cut(s) 669
Eco57I CTGAAG 2 cut(s) 110, 266
EcoRII CCWGG 2 cut(s) 320, 710
EcoRV GATATC 1 cut(s) 127
EcoT14I CCWWGG 1 cut(s) 357
ErhI CCWWGG 1 cut(s) 357
FaeI CATG 5 cut(s) 52, 310, 331, 346, 645
FaqI GGGAC 1 cut(s) 650
FatI CATG 5 cut(s) 48, 306, 327, 342, 641
Fnu4HI GCNGC 2 cut(s) 149, 336
Fsp4HI GCNGC 2 cut(s) 149, 336
FspBI CTAG 2 cut(s) 192, 389
GluI GCNGC 2 cut(s) 149, 336
GsaI CCCAGC 1 cut(s) 148
GsuI CTGGAG 3 cut(s) 20, 78, 221
HaeIII GGCC 5 cut(s) 21, 577, 626, 671, 715
HapII CCGG 1 cut(s) 608
HgaI GACGC 1 cut(s) 707
Hin1II CATG 5 cut(s) 52, 310, 331, 346, 645
HinfI GANTC 4 cut(s) 164, 176, 233, 398
HpaII CCGG 1 cut(s) 608
Hpy188I TCNGA 4 cut(s) 90, 238, 442, 513
Hpy188III TCNNGA 6 cut(s) 49, 110, 192, 230, 296, 467
HpyAV CCTTC 6 cut(s) 85, 107, 278, 325, 698, 742
HpyCH4III ACNGT 1 cut(s) 687
HpyCH4V TGCA 3 cut(s) 82, 151, 665
HpyF10VI GCNNNNNNNGC 2 cut(s) 148, 707
HpyF3I CTNAG 1 cut(s) 138
Hsp92II CATG 5 cut(s) 52, 310, 331, 346, 645
Kzo9I GATC 5 cut(s) 226, 277, 289, 437, 676
LmnI GCTCC 4 cut(s) 39, 59, 116, 758
Lsp1109I GCAGC 2 cut(s) 135, 347
LweI GCATC 1 cut(s) 115
MaeI CTAG 2 cut(s) 192, 389
MaeIII GTNAC 1 cut(s) 681
MalI GATC 5 cut(s) 228, 279, 291, 439, 678
MboI GATC 5 cut(s) 226, 277, 289, 437, 676
MboII GAAGA 2 cut(s) 121, 272
MfeI CAATTG 1 cut(s) 551
MflI RGATCY 4 cut(s) 226, 289, 437, 676
MlsI TGGCCA 1 cut(s) 715
MluCI AATT 6 cut(s) 187, 206, 350, 478, 551, 731
MluNI TGGCCA 1 cut(s) 715
MnlI CCTC 6 cut(s) 196, 275, 303, 355, 367, 627
Mox20I TGGCCA 1 cut(s) 715
MscI TGGCCA 1 cut(s) 715
MseI TTAA 1 cut(s) 775
Msp20I TGGCCA 1 cut(s) 715
MspA1I CMGCKG 1 cut(s) 148
MspI CCGG 1 cut(s) 608
MspR9I CCNGG 2 cut(s) 322, 712
MunI CAATTG 1 cut(s) 551
MvaI CCWGG 2 cut(s) 322, 712
MvnI CGCG 2 cut(s) 27, 701
MwoI GCNNNNNNNGC 2 cut(s) 148, 707
NdeII GATC 5 cut(s) 226, 277, 289, 437, 676
NlaIII CATG 5 cut(s) 52, 310, 331, 346, 645
NlaIV GGNNCC 3 cut(s) 291, 678, 709
NmeAIII GCCGAG 1 cut(s) 592
NmuCI GTSAC 1 cut(s) 681
PagI TCATGA 1 cut(s) 48
PcsI WCGNNNNNNNCGW 1 cut(s) 735
PfeI GAWTC 4 cut(s) 164, 176, 233, 398
PkrI GCNGC 2 cut(s) 150, 337
Psp6I CCWGG 2 cut(s) 320, 710
PspFI CCCAGC 1 cut(s) 144
PspGI CCWGG 2 cut(s) 320, 710
PspN4I GGNNCC 3 cut(s) 291, 678, 709
PstI CTGCAG 1 cut(s) 84
PstNI CAGNNNCTG 3 cut(s) 88, 161, 236
PsuI RGATCY 4 cut(s) 226, 289, 437, 676
PvuII CAGCTG 1 cut(s) 148
RsaI GTAC 1 cut(s) 743
RsaNI GTAC 1 cut(s) 742
SaqAI TTAA 1 cut(s) 775
SatI GCNGC 2 cut(s) 149, 336
Sau3AI GATC 5 cut(s) 226, 277, 289, 437, 676
ScaI AGTACT 1 cut(s) 743
ScrFI CCNGG 2 cut(s) 322, 712
SetI ASST 7 cut(s) 36, 87, 150, 288, 340, 447, 709
SfaNI GCATC 1 cut(s) 115
SfcI CTRYAG 1 cut(s) 80
SmlI CTYRAG 1 cut(s) 294
SmoI CTYRAG 1 cut(s) 294
Sse9I AATT 6 cut(s) 187, 206, 350, 478, 551, 731
SsiI CCGC 1 cut(s) 701
SspMI CTAG 2 cut(s) 192, 389
StyD4I CCNGG 2 cut(s) 320, 710
StyI CCWWGG 1 cut(s) 357
TaaI ACNGT 1 cut(s) 687
TaqI TCGA 3 cut(s) 466, 632, 738
TasI AATT 6 cut(s) 187, 206, 350, 478, 551, 731
TatI WGTACW 1 cut(s) 741
TfiI GAWTC 4 cut(s) 164, 176, 233, 398
Tru1I TTAA 1 cut(s) 775
Tru9I TTAA 1 cut(s) 775
TscAI CASTG 2 cut(s) 578, 690
TseFI GTSAC 1 cut(s) 681
TseI GCWGC 2 cut(s) 148, 335
Tsp45I GTSAC 1 cut(s) 681
TspDTI ATGAA 3 cut(s) 17, 344, 468
TspGWI ACGGA 3 cut(s) 404, 669, 771
TspRI CASTG 2 cut(s) 578, 690
XapI RAATTY 2 cut(s) 350, 731
XbaI TCTAGA 1 cut(s) 191
XspI CTAG 2 cut(s) 192, 389
ZrmI AGTACT 1 cut(s) 743
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.