Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0006161

Physical Location & Seq

Genomic Coordinates Forward (+)

21668761 .. 21681545

Gene Structure 12785 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3777 bp

ATGATGCAGCAGCATCAACCTCCGCCAGTCCCACTGCAATCGCAGACCGCCGGAACCCCAGCCGGAGTGCAGGTCCGCTGCGCCGGCTGCGGCAAGATCCTGACTTTGGACGCAGGGGTCACCGAATTCGCCTGCGACGCCTGCCAATTGCCCCAAATGTTGCCGCCCGAGCTGATGACCCGAGCCCAGGCTTATGCCCAGGCCCGCGCTCCGCCTCCGCTGCCACCCCCGCCGCCCCATGTTGCGCCTCACGGCGTTGATCCTTCGAAGATCCAGCTTCCCTGCGCTAATTGCAAGGCCATCCTTAACGTGCCTCATGGCCTCGCGAGGTTCCGGTGTCCTCAGTGCCAAGTTGACCTGGCTGTTGATGTCTCGAAGCTCCAGCAGTTCTTCCCGCCCCGTCTGCCCCTGCCTCCTCCACCCGAGGAGGTCAATGAGGTCGCCATCGAAGTAGAGCGAGAAGAGGATGAAGGTGGCATGGCAGGAGAAACATTTACAGACTATCGCCCTCCAAAACTATCCATTGGCCCTTCCCATCCAGATCCTGTTGTGGAAACATCGTCCTTGGCTGCTGTGCAGCCACCTGAGCCCACTTATGATCTGAAAATCAAGGATGATTTGGAAAATTTAAAGGCTTTGTCATGTTTACAGATTGAGACATTGGTCTATGCTTGTCAGAGACACCTGCAACATCTTCCAAGTGGTGAGAGGGCAGGATTCTTCGTTGGGGATGGAGCTGGTGTGGGGAAAGGTCGAACAATAGCAGGGTTAATATGGGAGAATTGGCACCGTGGGATGAGGAAAGCAGTGTGGGTTTCTGTTGGTTCAGACTTGAAGTTTGATGCGAGAAGAGACTTAGATGATGTAGGTGCAACTTGCATTGAAGTACACGCCTTGAACAAGCTGCCTTATTCCAAACTTGATTCTAAGTCTGTTGGCGTCAAGGAGGGCGTTGTTTTTCTGACATACAGCAGCCTCATTGCATCCTCTGAGAAAGGTCGTTCACGTATGGGGCAGCTTGTGCAGTGGTGTGGACCGGGATTTAATGGTCTTGTCGTATTTGATGAGTGCCACAAGGCCAAAAATCTGGTGCCTGAATCTGGAAGTCAGCCAACACGTACTGGTGAAGCAGTTCTTGAAATTCAGGCTAAGCTTCCTGAAGCTCGTGTTATTTATTGTTCAGCAACTGGTGCATCCGAACCACGAAATTTGGGTTACATGGTACGCCTTGGTCTTTGGGGCCCTGGAACATCTTTTTCTGATTTCCGTGAATTTCTAGGTGCTCTTGAGAAAGGAGGCGTTGGAGCACTCGAACTTGTTGCCATGGATATGAAAGCAAGGGGGATGTATGTATGCCGTACTCTTAGCTACAAAGGGGCAGAGTTTGAAGTTGTTGAAGCACCGCTAGAAGCTGAAATGATGGACATGTACGGAAAAGCAGCAGAATTTTGGACAGAACTGCGACAGGATATACTAACTGCATCTGAATTTCTTGCTAATGAAAAGCCTGTTTCTAGTCAAGTGTGGCGATTATATTGGGCCAGCCATCAGCGTTTCTTTAGACACATGTGCATGTCAGCTAAGGTGCCGGCTGCAGTAAGACTAGCTAAACAAGCATTGATGGATGGCAAGTGTGTGGTTATTGGCCTTCAGAGTACTGGAGAGGCGCGAACTGAGGAAGCTGTGACCAAATATGGTCTTGAACTTGATGATTTTATTTCCGGACCTCGAGAACTTTTACTGAAATTTGTTGAGGAAAATTATCCCTTGCCTGAAAAGCCTGAACCTCTCGAAGGAGAAGATAGTGTGAAGGAGCTGCAGCGGAAGAGGCACTCAGCAACTCCTGGTGTTTCAATGAAAGGGAGAGTGAGGAAAGTTGCAAAATGGAAACCTGCAAGTGATGATGAAAGCGATGAAGAATCTGAAACTGATTCTGCTCAGGAATCTACTGAATCTGATGATGAGTTTCAAATTTGTGAAATTTGCCAATCTGAGGAGGAAAGAAAGAAACTGCTTCATTGTTCCTGTTGTGGGCAACTGGTCCATGCTGCATGTCTTATCCCACCTGTGACAGATGAAGTGTCAGTAGACTGGTCATGCCATTCATGCAAGGAAAAAACAGATGAATTTTTGAAAAGAAGACAAGAGTATATAGCTGTTCTTACGAAGAGGTATGAAGAAGCTTTGGCTCGTAAGTTAAACATATTGGAGATCGTTCGTTCTTTGGATCTTCCAAACAATCCTTTGGATGATATCATTGATCAGCTTGGAGGCCCTGATAAAGTAGCAGAAATGACTGGAAGGCGAGGTATGCTTGTGAGGGCATCTGGTGGAAAAGGCGTTACCTATCAGGCACGGAACACGAAGGAAATTTCCATGGAGATGGTAAACATGCATGAGAAGCAGCTTTTCATGGACGGCAAGAAGTTGGTTGCCATCATTTCTAAAGCGAACCTAATGGATCTGCTGGCGTGTCTTTGCAGGCAGATAGAAGAGCTACAAATCAGAGACGAAGGGTACATCTTACTCTGGAACTTCCTTGGAGTGCTGATCGAGCGATTCAACAGTTTGGAAGAACTCACCGGTCAAATCAAGCCTCTGCTCCTGAATACAGCAGTGCTTATCTTTACTAACCTTGGTGGAGAGCGTCGATTTGCATCCATCGTAGCAAAGAGATTAGAATCTCTTGGAGCACTAACACAGGGTGACCGCAGGGCAGGGCTATCATTGAGTGCCTATAACTACGATAGTGCTTATGGAAAGAAGGCCTTGATGTTGATGTATAGAGGAATCATGGAGCAGGATTCTCTGCCTGTTGTGCCGCTAGGATGTTCATCAGAAAAACCGGAAACAATCCAAGATTTTGTTGAGAAGGCTAAAGCTGCTCTTGTTCTTGTAGGAATAGTTAGGGATGCTCATGGGAAAGATCATGGCAGGCTCGTTGGCCGTATAATTGAATCAGATATGCATGATGTTGGACGTTTCCTCAATCGGATTTTAGGAGTACCGCCTGATATACAGAATAGGCTGTTTGAGTGTTTTGTTAGCATTTTGGATCATATTGTCCATAATGCACGCATTGAAGGGAATCTGGACTCTGGGATTGTTGATATGAAAGCTAATGTTATAGAGCTACAAGGAACTCCAAAGACTGTTTATGTAGATCAAATGTCTGGAGCTTCGACAGTGCTTTTTACATTTACCCTGGATCGTGGGGTCACGTGGGAGTCTGCGAGTGCCATGCTTGAGGAGAAACAAAAGGATGGACTTGGTTCTGCCAATGATGGGTTCTATGAATCTAGGAGGGAATGGTTGGGAAGACGTCATGTCATATTAGCCTTTGAGAGTTCTACTTCCGGATTGTACAAGATAGTCCGTCCTGCGGTGGGAGAGTCAGTAAGAGAAATGCCTCTGTCGGAGCTAAAAAGCAAATACAGAAAAACATCAACATTAGAGAAGGCTCGTCATGGCTGGGAAGATGAATATGAAGTTTCGTCCAAACAGTGCATGCATGGACGCAATTGTAAGCTTGGCAACTTCTGTACTGTTGGCAGAAGACTGCAGGAAGTAAATGTATTAGGTGGCCTAATTCTTCCCGTCTGGGGCACTATTGAGAAGGCTCTTTCGAAGCAGCAACGGCAAAGCCACAAGCGACTACGTGTTGTGCGTATAGAAACCACGACAGATAACCGCAGAATCGTTGGTCTCTCTGTCCCAAATGCAGCAGTGGAATCCGTACTTCAAGATTTTGCATGGGTTCAAGAAATTGACGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002009 GO:0002165 GO:0003002 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0007154 GO:0007165 GO:0007166 GO:0007219 GO:0007275 GO:0007389 GO:0007444 GO:0007447 GO:0007450 GO:0007472 GO:0007476 GO:0007552 GO:0007560 GO:0008150 GO:0008587 GO:0009653 GO:0009791 GO:0009886 GO:0009887 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009953 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0035107 GO:0035114 GO:0035120 GO:0035220 GO:0035222 GO:0035239 GO:0035295 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0048190 GO:0048513 GO:0048518 GO:0048522 GO:0048563 GO:0048569 GO:0048707 GO:0048729 GO:0048731 GO:0048736 GO:0048737 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0060429 GO:0060562 GO:0065007 GO:0080090 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA_34 Helicase_C_4 PHD

Protein Analysis

1259

Amino Acids

139.59

Weight (kDa)

5.85

Isoelectric Point (pI)

51.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Zn_ribbon_FGT1_1	PF23547	23 - 57	4.7e-18	FORGETTER1 first zinc ribbon domain
Zn_ribbon_FGT1_1	PF23547	91 - 120	4.8e-10	FORGETTER1 first zinc ribbon domain
Zn_ribbon_FGT1_2	PF23548	91 - 124	6.5e-17	FORGETTER1 second zinc ribbon domain
AAA_34	PF13872	178 - 478	1.6e-132	P-loop containing NTP hydrolase pore-1
PHD	PF00628	659 - 703	5.7e-06	PHD-finger
Helicase_C_4	PF13871	747 - 817	2e-22	C-terminal domain on Strawberry notch homologue
Helicase_C_4	PF13871	873 - 1037	2.2e-37	C-terminal domain on Strawberry notch homologue
SBNO	PF25373	1074 - 1186	3e-19	SBNO alpha/beta domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0006161)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G79350
fragaria_vesca	FvH4_3g13920 FvH4_3g13920 FvH4_3g13920 FvH4_3g13920
malus_domestica	MD05G1239900.v1.1 MD10G1218000.v1.1
prunus_persica	Prupe.4G126100_v2.0.a1 Prupe.4G126100_v2.0.a1 Prupe.4G126100_v2.0.a1 Prupe.4G126100_v2.0.a1
pyrus_communis	pycom05g21600 pycom10g18380
rosa_chinensis	RchiOBHm_Chr5g0023151
rosa_laevigata	RLG00000032703
rosa_multiflora	Rmu_co8366291.1_g000001 Rmu_sc0007300.1_g000019 Rmu_sc0015109.1_g000002 Rmu_sc0033377.1_g000001
rosa_roxburghii	Rroxscaffold_1G00056190
rosa_rugosa	Rorug05G0077600
rosa_samantha	Rh5AG163600 Rh5BG163000 Rh5CG177800 Rh5DG164200
rosa_wichuraiana	Rw5G014760 Rw5G015070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	693
AasI	GACNNNNNNGTC	1 cut(s)	116
AatII	GACGTC	1 cut(s)	3325
Acc36I	ACCTGC	3 cut(s)	61, 693, 1900
AccB1I	GGYRCC	3 cut(s)	786, 1090, 1585
AccI	GTMKAC	1 cut(s)	2088
AccII	CGCG	3 cut(s)	207, 326, 1669
AccIII	TCCGGA	2 cut(s)	1721, 3356
AclWI	GGATC	8 cut(s)	91, 254, 265, 536, 2235, 2469, 3063, 3216
AcoI	YGGCCR	1 cut(s)	2944
AcuI	CTGAAG	2 cut(s)	1179, 1634
AcvI	CACGTG	1 cut(s)	3222
AcyI	GRCGYC	3 cut(s)	138, 939, 3322
AdeI	CACNNNGTG	1 cut(s)	2705
AflIII	ACRYGT	4 cut(s)	1115, 1425, 1566, 3658
AgeI	ACCGGT	1 cut(s)	2582
AhdI	GACNNNNNGTC	1 cut(s)	3326
AjnI	CCWGG	6 cut(s)	186, 198, 357, 1243, 1843, 3204
AjuI	GAANNNNNNNTTGG	2 cut(s)	3272, 3304
Alw21I	GWGCWC	3 cut(s)	1285, 1309, 2695
Alw26I	GTCTC	6 cut(s)	376, 650, 673, 846, 2502, 3710
AlwI	GGATC	8 cut(s)	91, 254, 265, 536, 2235, 2469, 3063, 3216
AlwNI	CAGNNNCTG	2 cut(s)	545, 1187
Ama87I	CYCGRG	4 cut(s)	167, 180, 422, 1728
Aor13HI	TCCGGA	2 cut(s)	1721, 3356
ApaI	GGGCCC	1 cut(s)	1244
ArsI	GACNNNNNNTTYG	2 cut(s)	821, 853
AsiGI	ACCGGT	1 cut(s)	2582
Asp700I	GAANNNNTTC	1 cut(s)	1131
AspLEI	GCGC	5 cut(s)	83, 209, 247, 287, 1669
AsuC2I	CCSGG	1 cut(s)	1038
AsuHPI	GGTGA	5 cut(s)	112, 716, 1136, 2572, 2717
AsuII	TTCGAA	2 cut(s)	266, 3626
AvaI	CYCGRG	4 cut(s)	167, 180, 422, 1728
AvaII	GGWCC	4 cut(s)	73, 1034, 1724, 2041
BaeGI	GKGCMC	2 cut(s)	1244, 3608
BanI	GGYRCC	3 cut(s)	786, 1090, 1585
BanII	GRGCYC	3 cut(s)	187, 591, 1244
BauI	CACGAG	1 cut(s)	1164
BbrPI	CACGTG	1 cut(s)	3222
BbsI	GAAGAC	3 cut(s)	2146, 3325, 3562
Bbv12I	GWGCWC	3 cut(s)	1285, 1309, 2695
BceAI	ACGGC	5 cut(s)	268, 1341, 2434, 2931, 3653
BcgI	CGANNNNNNTGC	4 cut(s)	1301, 1335, 3223, 3257
BciT130I	CCWGG	6 cut(s)	188, 200, 359, 1245, 1845, 3206
BclI	TGATCA	1 cut(s)	2260
BcnI	CCSGG	1 cut(s)	1038
BcoDI	GTCTC	6 cut(s)	376, 650, 673, 846, 2502, 3710
BfaI	CTAG	6 cut(s)	1277, 1406, 1515, 1604, 2825, 3300
BfmI	CTRYAG	3 cut(s)	1593, 1817, 3560
BfuAI	ACCTGC	3 cut(s)	61, 693, 1900
BlpI	GCTNAGC	1 cut(s)	1149
BmcAI	AGTACT	1 cut(s)	1657
Bme1390I	CCNGG	7 cut(s)	188, 200, 359, 1038, 1245, 1845, 3206
Bme18I	GGWCC	4 cut(s)	73, 1034, 1724, 2041
BmeRI	GACNNNNNGTC	1 cut(s)	3326
BmeT110I	CYCGRG	4 cut(s)	167, 180, 422, 1728
BmiI	GGNNCC	7 cut(s)	55, 332, 788, 1092, 1241, 1242, 1587
BmrFI	CCNGG	7 cut(s)	188, 200, 359, 1038, 1245, 1845, 3206
BmsI	GCATC	8 cut(s)	22, 832, 992, 1202, 1490, 2333, 2666, 2902
BoxI	GACNNNNGTC	1 cut(s)	662
BpiI	GAAGAC	3 cut(s)	2146, 3325, 3562
BpmI	CTGGAG	3 cut(s)	365, 1680, 3195
Bpu10I	CCTNAGC	3 cut(s)	585, 1581, 1938
Bpu1102I	GCTNAGC	1 cut(s)	1149
Bpu14I	TTCGAA	2 cut(s)	266, 3626
BpuEI	CTTGAG	2 cut(s)	1307, 3266
BpuMI	CCSGG	1 cut(s)	1038
BsaAI	YACGTR	4 cut(s)	1007, 1118, 3222, 3659
BsaBI	GATNNNNATC	3 cut(s)	2656, 2680, 2833
BsaHI	GRCGYC	3 cut(s)	138, 939, 3322
BsaI	GGTCTC	1 cut(s)	3710
BsaWI	WCCGGW	5 cut(s)	333, 1721, 2582, 2845, 3356
BsaXI	ACNNNNNCTCC	6 cut(s)	57, 87, 726, 756, 3381, 3411
Bse118I	RCCGGY	3 cut(s)	83, 1588, 2582
Bse1I	ACTGG	7 cut(s)	26, 1126, 1192, 1663, 2043, 2096, 2302
Bse3DI	GCAATG	1 cut(s)	978
Bse8I	GATNNNNATC	3 cut(s)	2656, 2680, 2833
BseAI	TCCGGA	2 cut(s)	1721, 3356
BseBI	CCWGG	6 cut(s)	188, 200, 359, 1245, 1845, 3206
BseJI	GATNNNNATC	3 cut(s)	2656, 2680, 2833
BseMI	GCAATG	1 cut(s)	978
BseMII	CTCAG	7 cut(s)	356, 576, 981, 1665, 1848, 1952, 1983
BseNI	ACTGG	7 cut(s)	26, 1126, 1192, 1663, 2043, 2096, 2302
BseRI	GAGGAG	4 cut(s)	405, 440, 2009, 3263
BseSI	GKGCMC	2 cut(s)	1244, 3608
BseYI	CCCAGC	2 cut(s)	58, 3471
BsgI	GTGCAG	3 cut(s)	89, 596, 1043
Bsh1236I	CGCG	3 cut(s)	207, 326, 1669
BshNI	GGYRCC	3 cut(s)	786, 1090, 1585
BshTI	ACCGGT	1 cut(s)	2582
BsiHKAI	GWGCWC	3 cut(s)	1285, 1309, 2695
BsiHKCI	CYCGRG	4 cut(s)	167, 180, 422, 1728
BslFI	GGGAC	2 cut(s)	14, 3698
BsmAI	GTCTC	6 cut(s)	376, 650, 673, 846, 2502, 3710
BsmBI	CGTCTC	1 cut(s)	2502
BsmFI	GGGAC	2 cut(s)	14, 3698
Bso31I	GGTCTC	1 cut(s)	3710
BsoBI	CYCGRG	4 cut(s)	167, 180, 422, 1728
Bsp119I	TTCGAA	2 cut(s)	266, 3626
Bsp120I	GGGCCC	1 cut(s)	1240
Bsp1286I	GDGCHC	7 cut(s)	187, 591, 1244, 1285, 1309, 2695, 3608
Bsp13I	TCCGGA	2 cut(s)	1721, 3356
Bsp1407I	TGTACA	1 cut(s)	3363
Bsp1720I	GCTNAGC	1 cut(s)	1149
Bsp19I	CCATGG	2 cut(s)	1323, 2376
Bsp68I	TCGCGA	1 cut(s)	326
BspCNI	CTCAG	7 cut(s)	355, 577, 982, 1666, 1847, 1951, 1984
BspEI	TCCGGA	2 cut(s)	1721, 3356
BspFNI	CGCG	3 cut(s)	207, 326, 1669
BspLI	GGNNCC	7 cut(s)	55, 332, 788, 1092, 1241, 1242, 1587
BspMAI	CTGCAG	3 cut(s)	1597, 1821, 3564
BspMI	ACCTGC	3 cut(s)	61, 693, 1900
BspPI	GGATC	8 cut(s)	91, 254, 265, 536, 2235, 2469, 3063, 3216
BspQI	GCTCTTC	1 cut(s)	2487
BspT104I	TTCGAA	2 cut(s)	266, 3626
BspT107I	GGYRCC	3 cut(s)	786, 1090, 1585
BspTNI	GGTCTC	1 cut(s)	3710
BsrDI	GCAATG	1 cut(s)	978
BsrFI	RCCGGY	3 cut(s)	83, 1588, 2582
BsrGI	TGTACA	1 cut(s)	3363
BsrI	ACTGG	7 cut(s)	26, 1126, 1192, 1663, 2043, 2096, 2302
BssAI	RCCGGY	3 cut(s)	83, 1588, 2582
BssNI	GRCGYC	3 cut(s)	138, 939, 3322
BssSI	CACGAG	1 cut(s)	1164
BssT1I	CCWWGG	6 cut(s)	564, 1228, 1323, 2376, 2539, 2635
Bst2BI	CACGAG	1 cut(s)	1164
Bst2UI	CCWGG	6 cut(s)	188, 200, 359, 1245, 1845, 3206
Bst4CI	ACNGT	6 cut(s)	791, 2567, 3154, 3187, 3504, 3547
Bst6I	CTCTTC	5 cut(s)	456, 844, 1820, 2162, 2487
BstACI	GRCGYC	3 cut(s)	138, 939, 3322
BstAPI	GCANNNNNTGC	2 cut(s)	1021, 1190
BstAUI	TGTACA	1 cut(s)	3363
BstBAI	YACGTR	4 cut(s)	1007, 1118, 3222, 3659
BstBI	TTCGAA	2 cut(s)	266, 3626
BstDSI	CCRYGG	3 cut(s)	790, 1323, 2376
BstEII	GGTNACC	2 cut(s)	118, 2705
BstENI	CCTNNNNNAGG	1 cut(s)	1791
BstFNI	CGCG	3 cut(s)	207, 326, 1669
BstHHI	GCGC	5 cut(s)	83, 209, 247, 287, 1669
BstMAI	GTCTC	6 cut(s)	376, 650, 673, 846, 2502, 3710
BstNI	CCWGG	6 cut(s)	188, 200, 359, 1245, 1845, 3206
BstNSI	RCATGY	6 cut(s)	1429, 1570, 1576, 2055, 2395, 3511
BstPAI	GACNNNNGTC	1 cut(s)	662
BstPI	GGTNACC	2 cut(s)	118, 2705
BstSCI	CCNGG	7 cut(s)	186, 198, 357, 1036, 1243, 1843, 3204
BstSFI	CTRYAG	3 cut(s)	1593, 1817, 3560
BstSLI	GKGCMC	2 cut(s)	1244, 3608
BstUI	CGCG	3 cut(s)	207, 326, 1669
BstV2I	GAAGAC	3 cut(s)	2146, 3325, 3562
BstX2I	RGATCY	5 cut(s)	96, 270, 541, 2227, 2461
BstXI	CCANNNNNNTGG	2 cut(s)	1087, 2383
BstYI	RGATCY	5 cut(s)	96, 270, 541, 2227, 2461
BtgI	CCRYGG	3 cut(s)	790, 1323, 2376
BtgZI	GCGATG	1 cut(s)	1926
BtsI	GCAGTG	5 cut(s)	32, 813, 1031, 2622, 3732
BtsIMutI	CAGTG	8 cut(s)	32, 350, 813, 1031, 2622, 3192, 3509, 3732
BtuMI	TCGCGA	1 cut(s)	326
BveI	ACCTGC	3 cut(s)	61, 693, 1900
CaiI	CAGNNNCTG	2 cut(s)	545, 1187
CfoI	GCGC	5 cut(s)	83, 209, 247, 287, 1669
Cfr10I	RCCGGY	3 cut(s)	83, 1588, 2582
CseI	GACGC	5 cut(s)	119, 146, 928, 2636, 3525
CspAI	ACCGGT	1 cut(s)	2582
CspCI	CAANNNNNGTGG	2 cut(s)	1191, 1226
DraI	TTTAAA	1 cut(s)	630
DraIII	CACNNNGTG	1 cut(s)	2705
DrdI	GACNNNNNNGTC	1 cut(s)	116
DriI	GACNNNNNGTC	1 cut(s)	3326
DseDI	GACNNNNNNGTC	1 cut(s)	116
EaeI	YGGCCR	1 cut(s)	2944
Eam1104I	CTCTTC	5 cut(s)	456, 844, 1820, 2162, 2487
Eam1105I	GACNNNNNGTC	1 cut(s)	3326
EarI	CTCTTC	5 cut(s)	456, 844, 1820, 2162, 2487
EciI	GGCGGA	2 cut(s)	12, 201
Eco130I	CCWWGG	6 cut(s)	564, 1228, 1323, 2376, 2539, 2635
Eco147I	AGGCCT	1 cut(s)	2768
Eco24I	GRGCYC	3 cut(s)	187, 591, 1244
Eco31I	GGTCTC	1 cut(s)	3710
Eco32I	GATATC	1 cut(s)	2254
Eco47I	GGWCC	4 cut(s)	73, 1034, 1724, 2041
Eco57I	CTGAAG	2 cut(s)	1179, 1634
Eco72I	CACGTG	1 cut(s)	3222
Eco88I	CYCGRG	4 cut(s)	167, 180, 422, 1728
Eco91I	GGTNACC	2 cut(s)	118, 2705
EcoNI	CCTNNNNNAGG	1 cut(s)	1791
EcoO109I	RGGNCCY	3 cut(s)	1240, 1241, 2273
EcoO65I	GGTNACC	2 cut(s)	118, 2705
EcoRI	GAATTC	1 cut(s)	125
EcoRII	CCWGG	6 cut(s)	186, 198, 357, 1243, 1843, 3204
EcoRV	GATATC	1 cut(s)	2254
EcoT14I	CCWWGG	6 cut(s)	564, 1228, 1323, 2376, 2539, 2635
EcoT22I	ATGCAT	3 cut(s)	2397, 2970, 3513
EcoT38I	GRGCYC	3 cut(s)	187, 591, 1244
ErhI	CCWWGG	6 cut(s)	564, 1228, 1323, 2376, 2539, 2635
Esp3I	CGTCTC	1 cut(s)	2502
FalI	AAGNNNNNCTT	6 cut(s)	1119, 1151, 2753, 2785, 2871, 2903
FaqI	GGGAC	2 cut(s)	14, 3698
FauI	CCCGC	3 cut(s)	212, 237, 402
FbaI	TGATCA	1 cut(s)	2260
FblI	GTMKAC	1 cut(s)	2088
FriOI	GRGCYC	3 cut(s)	187, 591, 1244
FspBI	CTAG	6 cut(s)	1277, 1406, 1515, 1604, 2825, 3300
GlaI	GCGC	5 cut(s)	82, 208, 246, 286, 1668
GsaI	CCCAGC	2 cut(s)	62, 3475
GsuI	CTGGAG	3 cut(s)	365, 1680, 3195
HgaI	GACGC	5 cut(s)	119, 146, 928, 2636, 3525
HhaI	GCGC	5 cut(s)	83, 209, 247, 287, 1669
Hin1I	GRCGYC	3 cut(s)	138, 939, 3322
Hin6I	GCGC	5 cut(s)	81, 207, 245, 285, 1667
HinP1I	GCGC	5 cut(s)	81, 207, 245, 285, 1667
HincII	GTYRAC	1 cut(s)	355
HindII	GTYRAC	1 cut(s)	355
HindIII	AAGCTT	3 cut(s)	1151, 2181, 3527
HphI	GGTGA	5 cut(s)	112, 716, 1136, 2572, 2717
Hpy166II	GTNNAC	7 cut(s)	355, 647, 889, 1004, 1034, 2089, 2389
Hpy8I	GTNNAC	7 cut(s)	355, 647, 889, 1004, 1034, 2089, 2389
Hpy99I	CGWCG	2 cut(s)	140, 2652
HpyCH4III	ACNGT	6 cut(s)	791, 2567, 3154, 3187, 3504, 3547
HpyCH4IV	ACGT	7 cut(s)	309, 1006, 1117, 2980, 3221, 3322, 3658
HpySE526I	ACGT	7 cut(s)	309, 1006, 1117, 2980, 3221, 3322, 3658
Hsp92I	GRCGYC	3 cut(s)	138, 939, 3322
HspAI	GCGC	5 cut(s)	81, 207, 245, 285, 1667
Kpn2I	TCCGGA	2 cut(s)	1721, 3356
KroI	GCCGGC	2 cut(s)	83, 1588
KroNI	GCCGGC	2 cut(s)	85, 1590
Ksp22I	TGATCA	1 cut(s)	2260
LguI	GCTCTTC	1 cut(s)	2487
LweI	GCATC	8 cut(s)	22, 832, 992, 1202, 1490, 2333, 2666, 2902
MaeI	CTAG	6 cut(s)	1277, 1406, 1515, 1604, 2825, 3300
MaeII	ACGT	7 cut(s)	309, 1006, 1117, 2980, 3221, 3322, 3658
MaeIII	GTNAC	7 cut(s)	118, 1214, 1684, 2068, 2341, 2705, 3217
MfeI	CAATTG	2 cut(s)	146, 3520
MflI	RGATCY	5 cut(s)	96, 270, 541, 2227, 2461
MhlI	GDGCHC	7 cut(s)	187, 591, 1244, 1285, 1309, 2695, 3608
MlyI	GAGTC	3 cut(s)	3089, 3236, 3401
MmeI	TCCRAC	3 cut(s)	1282, 2956, 3396
Mph1103I	ATGCAT	3 cut(s)	2397, 2970, 3513
MroI	TCCGGA	2 cut(s)	1721, 3356
MroNI	GCCGGC	2 cut(s)	83, 1588
MroXI	GAANNNNTTC	1 cut(s)	1131
MseI	TTAA	5 cut(s)	306, 629, 770, 1044, 2198
MslI	CAYNNNNRTG	3 cut(s)	1328, 1571, 2381
MspA1I	CMGCKG	3 cut(s)	78, 220, 1822
MspR9I	CCNGG	7 cut(s)	188, 200, 359, 1038, 1245, 1845, 3206
MunI	CAATTG	2 cut(s)	146, 3520
MvaI	CCWGG	6 cut(s)	188, 200, 359, 1245, 1845, 3206
MvnI	CGCG	3 cut(s)	207, 326, 1669
NaeI	GCCGGC	2 cut(s)	85, 1590
NciI	CCSGG	1 cut(s)	1038
NcoI	CCATGG	2 cut(s)	1323, 2376
NgoMIV	GCCGGC	2 cut(s)	83, 1588
NlaIV	GGNNCC	7 cut(s)	55, 332, 788, 1092, 1241, 1242, 1587
NmuCI	GTSAC	5 cut(s)	118, 1684, 2068, 2705, 3217
NruI	TCGCGA	1 cut(s)	326
NsiI	ATGCAT	3 cut(s)	2397, 2970, 3513
NspI	RCATGY	6 cut(s)	1429, 1570, 1576, 2055, 2395, 3511
NspV	TTCGAA	2 cut(s)	266, 3626
PaeI	GCATGC	1 cut(s)	3511
PaeR7I	CTCGAG	1 cut(s)	1728
PaqCI	CACCTGC	1 cut(s)	693
PceI	AGGCCT	1 cut(s)	2768
PciI	ACATGT	2 cut(s)	1425, 1566
PciSI	GCTCTTC	1 cut(s)	2487
PcsI	WCGNNNNNNNCGW	1 cut(s)	3664
PdiI	GCCGGC	2 cut(s)	85, 1590
PdmI	GAANNNNTTC	1 cut(s)	1131
PinAI	ACCGGT	1 cut(s)	2582
PleI	GAGTC	3 cut(s)	3089, 3235, 3400
PmaCI	CACGTG	1 cut(s)	3222
PmlI	CACGTG	1 cut(s)	3222
PpsI	GAGTC	3 cut(s)	3089, 3235, 3400
Ppu21I	YACGTR	4 cut(s)	1007, 1118, 3222, 3659
PscI	ACATGT	2 cut(s)	1425, 1566
PshAI	GACNNNNGTC	1 cut(s)	662
Psp6I	CCWGG	6 cut(s)	186, 198, 357, 1243, 1843, 3204
PspCI	CACGTG	1 cut(s)	3222
PspEI	GGTNACC	2 cut(s)	118, 2705
PspFI	CCCAGC	2 cut(s)	58, 3471
PspGI	CCWGG	6 cut(s)	186, 198, 357, 1243, 1843, 3204
PspN4I	GGNNCC	7 cut(s)	55, 332, 788, 1092, 1241, 1242, 1587
PspOMI	GGGCCC	1 cut(s)	1240
PstI	CTGCAG	3 cut(s)	1597, 1821, 3564
PstNI	CAGNNNCTG	2 cut(s)	545, 1187
PsuI	RGATCY	5 cut(s)	96, 270, 541, 2227, 2461
RruI	TCGCGA	1 cut(s)	326
RseI	CAYNNNNRTG	3 cut(s)	1328, 1571, 2381
SapI	GCTCTTC	1 cut(s)	2487
SaqAI	TTAA	5 cut(s)	306, 629, 770, 1044, 2198
ScaI	AGTACT	1 cut(s)	1657
SchI	GAGTC	3 cut(s)	3089, 3236, 3401
ScrFI	CCNGG	7 cut(s)	188, 200, 359, 1038, 1245, 1845, 3206
SduI	GDGCHC	7 cut(s)	187, 591, 1244, 1285, 1309, 2695, 3608
SfaNI	GCATC	8 cut(s)	22, 832, 992, 1202, 1490, 2333, 2666, 2902
SfcI	CTRYAG	3 cut(s)	1593, 1817, 3560
Sfr274I	CTCGAG	1 cut(s)	1728
SfuI	TTCGAA	2 cut(s)	266, 3626
SinI	GGWCC	4 cut(s)	73, 1034, 1724, 2041
SlaI	CTCGAG	1 cut(s)	1728
SmiMI	CAYNNNNRTG	3 cut(s)	1328, 1571, 2381
SmlI	CTYRAG	3 cut(s)	1286, 1728, 3245
SmoI	CTYRAG	3 cut(s)	1286, 1728, 3245
SphI	GCATGC	1 cut(s)	3511
SseBI	AGGCCT	1 cut(s)	2768
SspMI	CTAG	6 cut(s)	1277, 1406, 1515, 1604, 2825, 3300
StuI	AGGCCT	1 cut(s)	2768
StyD4I	CCNGG	7 cut(s)	186, 198, 357, 1036, 1243, 1843, 3204
StyI	CCWWGG	6 cut(s)	564, 1228, 1323, 2376, 2539, 2635
TaaI	ACNGT	6 cut(s)	791, 2567, 3154, 3187, 3504, 3547
TaiI	ACGT	7 cut(s)	312, 1009, 1120, 2983, 3224, 3325, 3661
TatI	WGTACW	4 cut(s)	886, 1655, 3363, 3542
TauI	GCSGC	4 cut(s)	93, 166, 235, 2824
Tru1I	TTAA	5 cut(s)	306, 629, 770, 1044, 2198
Tru9I	TTAA	5 cut(s)	306, 629, 770, 1044, 2198
TscAI	CASTG	8 cut(s)	39, 350, 813, 1031, 2622, 3192, 3509, 3732
TseFI	GTSAC	5 cut(s)	118, 1684, 2068, 2705, 3217
Tsp45I	GTSAC	5 cut(s)	118, 1684, 2068, 2705, 3217
TspGWI	ACGGA	5 cut(s)	1256, 1446, 2371, 3365, 3724
TspRI	CASTG	8 cut(s)	39, 350, 813, 1031, 2622, 3192, 3509, 3732
VpaK11BI	GGWCC	4 cut(s)	73, 1034, 1724, 2041
XagI	CCTNNNNNAGG	1 cut(s)	1791
XceI	RCATGY	6 cut(s)	1429, 1570, 1576, 2055, 2395, 3511
XhoI	CTCGAG	1 cut(s)	1728
XmiI	GTMKAC	1 cut(s)	2088
XmnI	GAANNNNTTC	1 cut(s)	1131
XspI	CTAG	6 cut(s)	1277, 1406, 1515, 1604, 2825, 3300
ZraI	GACGTC	1 cut(s)	3323
ZrmI	AGTACT	1 cut(s)	1657
Zsp2I	ATGCAT	3 cut(s)	2397, 2970, 3513

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.