pycom10g20240

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. The coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0012024
Physical Location & Seq
Forward (+)
23183262 .. 23185851
2590 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1083 bp
ATGGACCAGCTTGTGTTACGAACTCATAAAATAATTTCTCTTTGGGTTGGCCCAAGTTTGGAATTAATCTTACGCATCATCATAAATCCTTCTAAAGTTTATAATAAACCGGCCCCTCGACAGCATTCGTCCTCCACGCTTTTGTCTCAGATCTGCAACTGTGAGAAACCAAATCGAAATCTCTCAACAATGGCAGCAGTGGCAGCAGTGGCAGCAGGACCGGACCACCTCTTCAACCTCCGCAACAACTTCTACTTGGGAGCCTACCAGGCCTCCATTAACAACAGCGACATTCCCAACCTCTCCCCCGACGAAGCCGTCGAGCGCGACTGCCTCGTCTTCCGCTCTTACATCGCCCTCGGCAGCTACCAGCTCGTCATCAATGAGATCGACGCCTCCGCCGCCACGCCTCTCCAGGCCGTCAAGCTCCTCGCCCTCTACCTCTCTGGCCCCGATTTCAAGGAATCGGCGATCTCGAATTTGAAGGAGTTGTTGGCGGATCCGGCCGTCGGAAACAACCCTACTCTGAGGCTGATTGCTGGGACGGTCTTCATGCATGAGCAGGACTACAATGAAGCTCTCAAATACACCAACGCCGGAGGCACTATGGAACTGAATGCATTGACTATCCAGATATTCCTTAAAATGCATAGGTCAGATTATGCTGAGAGACAGCTGCGGCTCATGCAACAGGTTGACGAGGACCACACACTAACTCAACTTGCAATGGCATGGTTAAGTCTGGCAGTGGGTGGTTCCAAGATACAGGAGGCATATCTCATCTTCCAAGATTTATCTGAGAAGTCTCAGGTTACGAGTTTGATCCTCAATGGAAAGGCTGTTTGCTGTATGCATATGGGAAACTTTGATGAAGCTGAAACACTACTTCTTGAAGCACTGCAAAAGGACGCAAAGGATCCATCAACTCTAGCCAACCTTGTTGTATGCTGTCTTCACCTTGGAAAACCATCTTCTCGTTTCCTCAGCCAGTTGAAAATTGGTCACCCAGATCACTTGCTCGTCAAACGGGTCTTGGAGGCAGAACAGAGCTTTAACCTAGCTGCTCTTCAAGCAGCTGGTTGA

Protein Analysis

361

Amino Acids

39.63

Weight (kDa)

5.93

Isoelectric Point (pI)

38.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Coatomer_E PF04733 74 - 357 9.5e-128 Coatomer epsilon subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012024)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30630 AT2G34840 AT2G34840
fragaria_vesca FvH4_3g11400 FvH4_3g11400
malus_domestica MD05G1263700.v1.1 MD10G1241400.v1.1
prunus_persica Prupe.4G101800_v2.0.a1
pyrus_communis pycom05g24770 pycom10g20240
rosa_chinensis RchiOBHm_Chr5g0018341
rosa_laevigata RLG00000032353
rosa_multiflora Rmu_sc0004464.1_g000001
rosa_roxburghii Rroxscaffold_1G00059400
rosa_rugosa Rorug05G0041600
rosa_samantha Rh5BG133500 Rh5CG145300 Rh5DG134900
rosa_wichuraiana Rw5G011780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 102
AasI GACNNNNNNGTC 2 cut(s) 317, 335
AccBSI CCGCTC 1 cut(s) 345
AccII CGCG 1 cut(s) 327
AciI CCGC 6 cut(s) 241, 343, 399, 402, 497, 679
AclWI GGATC 5 cut(s) 494, 507, 817, 911, 924
AcoI YGGCCR 1 cut(s) 504
AcsI RAATTY 1 cut(s) 478
AcyI GRCGYC 1 cut(s) 393
AfiI CCNNNNNNNGG 2 cut(s) 58, 509
AgsI TTSAA 6 cut(s) 235, 460, 484, 893, 994, 1070
AjnI CCWGG 2 cut(s) 267, 414
AjuI GAANNNNNNNTTGG 2 cut(s) 476, 508
Alw26I GTCTC 3 cut(s) 150, 664, 810
AlwI GGATC 5 cut(s) 494, 507, 817, 911, 924
AoxI GGCC 6 cut(s) 49, 111, 270, 417, 448, 504
ApeKI GCWGC 7 cut(s) 194, 203, 212, 363, 676, 1061, 1073
ApoI RAATTY 1 cut(s) 478
AseI ATTAAT 1 cut(s) 65
AspLEI GCGC 1 cut(s) 327
AspS9I GGNCC 7 cut(s) 4, 50, 112, 218, 223, 449, 703
AsuHPI GGTGA 2 cut(s) 947, 995
AvaII GGWCC 4 cut(s) 4, 218, 223, 703
BamHI GGATCC 2 cut(s) 499, 916
BarI GAAGNNNNNNTAC 2 cut(s) 936, 968
BbsI GAAGAC 3 cut(s) 331, 541, 944
BbvCI CCTCAGC 1 cut(s) 983
BbvI GCAGC 6 cut(s) 206, 215, 224, 375, 663, 1048
BccI CCATC 2 cut(s) 928, 976
BceAI ACGGC 3 cut(s) 302, 404, 491
BciT130I CCWGG 2 cut(s) 269, 416
BcoDI GTCTC 3 cut(s) 150, 664, 810
BfaI CTAG 2 cut(s) 929, 1058
BglI GCCNNNNNGGC 1 cut(s) 269
BglII AGATCT 1 cut(s) 150
BisI GCNGC 9 cut(s) 195, 204, 213, 364, 402, 677, 680, 1062, 1074
BlsI GCNGC 9 cut(s) 196, 205, 214, 365, 403, 678, 681, 1063, 1075
Bme1390I CCNGG 2 cut(s) 269, 416
Bme18I GGWCC 4 cut(s) 4, 218, 223, 703
BmgT120I GGNCC 7 cut(s) 4, 50, 112, 218, 223, 449, 703
BmiI GGNNCC 6 cut(s) 114, 262, 451, 501, 757, 918
BmrFI CCNGG 2 cut(s) 269, 416
BmsI GCATC 1 cut(s) 84
BpiI GAAGAC 3 cut(s) 331, 541, 944
BpmI CTGGAG 1 cut(s) 398
Bpu10I CCTNAGC 1 cut(s) 983
BsaHI GRCGYC 1 cut(s) 393
BsaJI CCNNGG 2 cut(s) 358, 958
BsaWI WCCGGW 1 cut(s) 220
BsaXI ACNNNNNCTCC 2 cut(s) 257, 287
Bsc4I CCNNNNNNNGG 2 cut(s) 58, 509
Bse118I RCCGGY 1 cut(s) 109
Bse1I ACTGG 1 cut(s) 988
Bse3DI GCAATG 1 cut(s) 732
BseBI CCWGG 2 cut(s) 269, 416
BseDI CCNNGG 2 cut(s) 358, 958
BseLI CCNNNNNNNGG 2 cut(s) 58, 509
BseMI GCAATG 1 cut(s) 732
BseMII CTCAG 6 cut(s) 161, 518, 657, 789, 821, 997
BseNI ACTGG 1 cut(s) 988
BseRI GAGGAG 1 cut(s) 419
BseX3I CGGCCG 1 cut(s) 504
BseXI GCAGC 6 cut(s) 206, 215, 224, 375, 663, 1048
BseYI CCCAGC 1 cut(s) 539
Bsh1236I CGCG 1 cut(s) 327
Bsh1285I CGRYCG 1 cut(s) 507
BshFI GGCC 6 cut(s) 51, 113, 272, 419, 450, 506
BsiEI CGRYCG 1 cut(s) 507
BsiSI CCGG 4 cut(s) 110, 221, 503, 597
BslFI GGGAC 1 cut(s) 556
BslI CCNNNNNNNGG 2 cut(s) 58, 509
BsmAI GTCTC 3 cut(s) 150, 664, 810
BsmFI GGGAC 1 cut(s) 556
BsmI GAATGC 2 cut(s) 124, 622
BsnI GGCC 6 cut(s) 51, 113, 272, 419, 450, 506
Bsp143I GATC 7 cut(s) 150, 387, 471, 499, 822, 916, 1009
BspACI CCGC 6 cut(s) 241, 343, 399, 402, 497, 679
BspANI GGCC 6 cut(s) 51, 113, 272, 419, 450, 506
BspCNI CTCAG 6 cut(s) 160, 519, 658, 790, 820, 996
BspFNI CGCG 1 cut(s) 327
BspLI GGNNCC 6 cut(s) 114, 262, 451, 501, 757, 918
BspPI GGATC 5 cut(s) 494, 507, 817, 911, 924
BspQI GCTCTTC 1 cut(s) 1071
BsrBI CCGCTC 1 cut(s) 345
BsrDI GCAATG 1 cut(s) 732
BsrFI RCCGGY 1 cut(s) 109
BsrI ACTGG 1 cut(s) 988
BssAI RCCGGY 1 cut(s) 109
BssECI CCNNGG 2 cut(s) 358, 958
BssMI GATC 7 cut(s) 150, 387, 471, 499, 822, 916, 1009
BssNI GRCGYC 1 cut(s) 393
BssT1I CCWWGG 1 cut(s) 958
Bst2UI CCWGG 2 cut(s) 269, 416
Bst4CI ACNGT 2 cut(s) 161, 547
Bst6I CTCTTC 2 cut(s) 236, 1071
BstACI GRCGYC 1 cut(s) 393
BstDEI CTNAG 6 cut(s) 147, 527, 666, 798, 807, 983
BstEII GGTNACC 1 cut(s) 1001
BstFNI CGCG 1 cut(s) 327
BstHHI GCGC 1 cut(s) 327
BstKTI GATC 7 cut(s) 153, 390, 474, 502, 825, 919, 1012
BstMAI GTCTC 3 cut(s) 150, 664, 810
BstMBI GATC 7 cut(s) 150, 387, 471, 499, 822, 916, 1009
BstMCI CGRYCG 1 cut(s) 507
BstMWI GCNNNNNNNGC 9 cut(s) 200, 203, 209, 212, 269, 401, 503, 685, 1070
BstNI CCWGG 2 cut(s) 269, 416
BstPI GGTNACC 1 cut(s) 1001
BstSCI CCNGG 2 cut(s) 267, 414
BstUI CGCG 1 cut(s) 327
BstV1I GCAGC 6 cut(s) 206, 215, 224, 375, 663, 1048
BstV2I GAAGAC 3 cut(s) 331, 541, 944
BstX2I RGATCY 3 cut(s) 150, 499, 916
BstYI RGATCY 3 cut(s) 150, 499, 916
BstZI CGGCCG 1 cut(s) 504
BsuRI GGCC 6 cut(s) 51, 113, 272, 419, 450, 506
BtgZI GCGATG 1 cut(s) 337
BtsI GCAGTG 4 cut(s) 204, 213, 753, 896
BtsIMutI CAGTG 4 cut(s) 204, 213, 753, 896
CfoI GCGC 1 cut(s) 327
Cfr10I RCCGGY 1 cut(s) 109
Cfr13I GGNCC 7 cut(s) 4, 50, 112, 218, 223, 449, 703
CseI GACGC 2 cut(s) 401, 917
CviAII CATG 4 cut(s) 553, 557, 685, 732
DdeI CTNAG 6 cut(s) 147, 527, 666, 798, 807, 983
DpnI GATC 7 cut(s) 152, 389, 473, 501, 824, 918, 1011
DpnII GATC 7 cut(s) 150, 387, 471, 499, 822, 916, 1009
DrdI GACNNNNNNGTC 2 cut(s) 317, 335
DseDI GACNNNNNNGTC 2 cut(s) 317, 335
EaeI YGGCCR 1 cut(s) 504
EagI CGGCCG 1 cut(s) 504
Eam1104I CTCTTC 2 cut(s) 236, 1071
EarI CTCTTC 2 cut(s) 236, 1071
EciI GGCGGA 2 cut(s) 388, 512
EclXI CGGCCG 1 cut(s) 504
Eco130I CCWWGG 1 cut(s) 958
Eco147I AGGCCT 1 cut(s) 272
Eco47I GGWCC 4 cut(s) 4, 218, 223, 703
Eco52I CGGCCG 1 cut(s) 504
Eco91I GGTNACC 1 cut(s) 1001
EcoO65I GGTNACC 1 cut(s) 1001
EcoRII CCWGG 2 cut(s) 267, 414
EcoT14I CCWWGG 1 cut(s) 958
EcoT22I ATGCAT 4 cut(s) 558, 622, 651, 855
ErhI CCWWGG 1 cut(s) 958
FaeI CATG 4 cut(s) 556, 560, 688, 735
FaqI GGGAC 1 cut(s) 556
FatI CATG 4 cut(s) 552, 556, 684, 731
FauNDI CATATG 1 cut(s) 855
Fnu4HI GCNGC 9 cut(s) 195, 204, 213, 364, 402, 677, 680, 1062, 1074
Fsp4HI GCNGC 9 cut(s) 195, 204, 213, 364, 402, 677, 680, 1062, 1074
FspBI CTAG 2 cut(s) 929, 1058
GlaI GCGC 1 cut(s) 326
GluI GCNGC 9 cut(s) 195, 204, 213, 364, 402, 677, 680, 1062, 1074
GsaI CCCAGC 1 cut(s) 543
GsuI CTGGAG 1 cut(s) 398
HaeIII GGCC 6 cut(s) 51, 113, 272, 419, 450, 506
HapII CCGG 4 cut(s) 110, 221, 503, 597
HgaI GACGC 2 cut(s) 401, 917
HhaI GCGC 1 cut(s) 327
Hin1I GRCGYC 1 cut(s) 393
Hin1II CATG 4 cut(s) 556, 560, 688, 735
Hin6I GCGC 1 cut(s) 325
HinP1I GCGC 1 cut(s) 325
HincII GTYRAC 1 cut(s) 697
HindII GTYRAC 1 cut(s) 697
HinfI GANTC 1 cut(s) 464
HpaII CCGG 4 cut(s) 110, 221, 503, 597
HphI GGTGA 2 cut(s) 947, 995
Hpy166II GTNNAC 1 cut(s) 697
Hpy188I TCNGA 5 cut(s) 150, 512, 528, 658, 799
Hpy188III TCNNGA 3 cut(s) 475, 631, 890
Hpy8I GTNNAC 1 cut(s) 697
Hpy99I CGWCG 4 cut(s) 314, 323, 395, 512
HpyAV CCTTC 2 cut(s) 99, 478
HpyCH4III ACNGT 2 cut(s) 161, 547
HpyCH4V TGCA 8 cut(s) 156, 556, 620, 649, 688, 725, 853, 901
HpyF10VI GCNNNNNNNGC 9 cut(s) 200, 203, 209, 212, 269, 401, 503, 685, 1070
HpyF3I CTNAG 6 cut(s) 147, 527, 666, 798, 807, 983
Hsp92I GRCGYC 1 cut(s) 393
Hsp92II CATG 4 cut(s) 556, 560, 688, 735
HspAI GCGC 1 cut(s) 325
Kzo9I GATC 7 cut(s) 150, 387, 471, 499, 822, 916, 1009
LguI GCTCTTC 1 cut(s) 1071
LmnI GCTCC 2 cut(s) 260, 432
Lsp1109I GCAGC 6 cut(s) 206, 215, 224, 375, 663, 1048
LweI GCATC 1 cut(s) 84
MaeI CTAG 2 cut(s) 929, 1058
MaeIII GTNAC 3 cut(s) 15, 811, 1001
MalI GATC 7 cut(s) 152, 389, 473, 501, 824, 918, 1011
MbiI CCGCTC 1 cut(s) 345
MboI GATC 7 cut(s) 150, 387, 471, 499, 822, 916, 1009
MboII GAAGA 7 cut(s) 223, 331, 541, 775, 944, 963, 1058
MflI RGATCY 3 cut(s) 150, 499, 916
MluCI AATT 4 cut(s) 33, 62, 478, 996
MmeI TCCRAC 1 cut(s) 490
Mph1103I ATGCAT 4 cut(s) 558, 622, 651, 855
MseI TTAA 5 cut(s) 65, 279, 642, 737, 1053
MspA1I CMGCKG 2 cut(s) 676, 1076
MspI CCGG 4 cut(s) 110, 221, 503, 597
MspR9I CCNGG 2 cut(s) 269, 416
Mva1269I GAATGC 2 cut(s) 124, 622
MvaI CCWGG 2 cut(s) 269, 416
MvnI CGCG 1 cut(s) 327
MwoI GCNNNNNNNGC 9 cut(s) 200, 203, 209, 212, 269, 401, 503, 685, 1070
NdeI CATATG 1 cut(s) 855
NdeII GATC 7 cut(s) 150, 387, 471, 499, 822, 916, 1009
NlaIII CATG 4 cut(s) 556, 560, 688, 735
NlaIV GGNNCC 6 cut(s) 114, 262, 451, 501, 757, 918
NmeAIII GCCGAG 1 cut(s) 339
NmuCI GTSAC 1 cut(s) 1001
NsiI ATGCAT 4 cut(s) 558, 622, 651, 855
PceI AGGCCT 1 cut(s) 272
PciSI GCTCTTC 1 cut(s) 1071
PcsI WCGNNNNNNNCGW 2 cut(s) 318, 473
PctI GAATGC 2 cut(s) 124, 622
PfeI GAWTC 1 cut(s) 464
PkrI GCNGC 9 cut(s) 196, 205, 214, 365, 403, 678, 681, 1063, 1075
PshBI ATTAAT 1 cut(s) 65
PsiI TTATAA 1 cut(s) 102
Psp6I CCWGG 2 cut(s) 267, 414
PspEI GGTNACC 1 cut(s) 1001
PspFI CCCAGC 1 cut(s) 539
PspGI CCWGG 2 cut(s) 267, 414
PspN4I GGNNCC 6 cut(s) 114, 262, 451, 501, 757, 918
PspPI GGNCC 7 cut(s) 4, 50, 112, 218, 223, 449, 703
PsuI RGATCY 3 cut(s) 150, 499, 916
PvuII CAGCTG 2 cut(s) 676, 1076
SapI GCTCTTC 1 cut(s) 1071
SaqAI TTAA 5 cut(s) 65, 279, 642, 737, 1053
SatI GCNGC 9 cut(s) 195, 204, 213, 364, 402, 677, 680, 1062, 1074
Sau3AI GATC 7 cut(s) 150, 387, 471, 499, 822, 916, 1009
Sau96I GGNCC 7 cut(s) 4, 50, 112, 218, 223, 449, 703
ScrFI CCNGG 2 cut(s) 269, 416
SfaNI GCATC 1 cut(s) 84
SinI GGWCC 4 cut(s) 4, 218, 223, 703
Sse9I AATT 4 cut(s) 33, 62, 478, 996
SseBI AGGCCT 1 cut(s) 272
SsiI CCGC 6 cut(s) 241, 343, 399, 402, 497, 679
SspMI CTAG 2 cut(s) 929, 1058
StuI AGGCCT 1 cut(s) 272
StyD4I CCNGG 2 cut(s) 267, 414
StyI CCWWGG 1 cut(s) 958
TaaI ACNGT 2 cut(s) 161, 547
TaqI TCGA 5 cut(s) 118, 175, 321, 390, 476
TasI AATT 4 cut(s) 33, 62, 478, 996
TauI GCSGC 2 cut(s) 404, 682
TfiI GAWTC 1 cut(s) 464
Tru1I TTAA 5 cut(s) 65, 279, 642, 737, 1053
Tru9I TTAA 5 cut(s) 65, 279, 642, 737, 1053
TscAI CASTG 4 cut(s) 204, 213, 753, 903
TseFI GTSAC 1 cut(s) 1001
TseI GCWGC 7 cut(s) 194, 203, 212, 363, 676, 1061, 1073
Tsp45I GTSAC 1 cut(s) 1001
TspDTI ATGAA 3 cut(s) 541, 588, 885
TspRI CASTG 4 cut(s) 204, 213, 753, 903
VpaK11BI GGWCC 4 cut(s) 4, 218, 223, 703
VspI ATTAAT 1 cut(s) 65
XapI RAATTY 1 cut(s) 478
XcmI CCANNNNNNNNNTGG 1 cut(s) 995
XspI CTAG 2 cut(s) 929, 1058
Zsp2I ATGCAT 4 cut(s) 558, 622, 651, 855
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.