pycom10g20330
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0010362
Physical Location & Seq
Forward (+)
23249432 .. 23253528
4097 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1524 bp
ATGTGCAGCTTGTTCATGCCAATTACTAGGATGGAGAATTATCAAGGTGATTTAACCGACATACTAAGAGCTGGTACAACTACTGATAACGTTGTAGCTTATCCGTCTGATCATCAAGAACCTTACACCGACACGTGGCACTTCCCATCGGATCATCCCATGATATTTCCATCATCTTCATTAGCCATGGAAGAAGAAGAAGCTAAGGACAACTTTGGCGATCCATTTTCGTCGTACATGCGCGATCCGCTTCTTAACGAGCTAGACATATCAAGCTCCAGCCTTTTCAGCAGCCCCAGTTGTACAGCTCGTGTGGAGGAAGGTGTCACAACTACTACTTTTGGTGGCGGTGGCGGTCATGATCATGTACTTAGTCTTGGGCATCACAACCATCAAAGTGGTGGTGTTGTGGTTGATGAAGATCCAATAAAGAGGCCTTGCAACATATTCTCACAGATGCTACAGATCTCTCCAAGTGCAAAGATACCCATGTCGCCGTGTGATTCGCCGGTGATCGGTGGAGCAGTGTCGTCGTCTCCTAGGGGGATAAAAACCTCACCAGATTCTGCTGCCATGGTTGCAAGCGATATGATTATTAATGGGAACAGCTCATCAGCAGCAACAGGTTGCTTGCTCGGGAACACCGGGATGCAGATCTCATCTCCACGGAATCCGGGGATCAAGCGAAGGAAGAGCCAGGCAAAGAAGGTGGTGTGCATTCCAGCGCCAGCGGCTGCAAACAGCAGGCCTACTGGAGAAGTAGTTCCGTCTGATCTGTGGGCATGGAGAAAGTACGGTCAGAAACCCATCAAAGGGTCACCTTTTCCAAGGGGCTACTACAGATGTAGCAGCTCAAAGGGTTGCCCAGCAAGAAAACAAGTAGAGCGTAGCCGGACGGATCCAAACATGTTGGTAATCACCTACACTTCTGAACACAACCATCCATGGCCTACTCAGAGAAATGCCCTAGCTGGCTCAACCAGATCCCAGCCATCCAAAAACGGCGCTGCCGCCGCCTTGAAGGTCTCTCCCAATTGCTCTCATCAGCCTCTTCAAAAGCCAACTAGCCCAACAAAGGAAGAACAAAATGAGAATGTGAATGATGATAACCAAGTGTGTCCAACAGTTATCACCGCTGGAAGTGCTTCAACAGTGAAGGAAGAATTTGAGGGCATTGAGAAGCAATTAGAGCTTGATCTTCATCACCAGCACTTAGGGTTTGCTTATAGACCATCAATGCCTGATGAGCAGTCCAACCAGTCTCACCATCCTGAGGACTTCTTTGCTGATTTAGGAGAAATTGAGGCTGACCCACTAAACCTCTTGTTTTCACAATGTTTCTCAGCGGATGAGCAGCAGCAAAAGGCAGCCAAGGATGCAGCGCCCTTAGATCCATTCAATCTCTTTGATAATTGGTCAGGATCGATCACAACACCAACAACACTTCATTTGGTGAAGCTAAGAGGCAGTTATAACAAGGCCAAGACTAATCACCATGAAAAGATATCAATCAACCCCCACTAA

Protein Analysis

508

Amino Acids

55.22

Weight (kDa)

5.98

Isoelectric Point (pI)

56.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 258 - 315 2.1e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010362)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30650 AT2G34830 AT2G34830
fragaria_vesca FvH4_3g11140
malus_domestica MD05G1265200.v1.1 MD10G1243000.v1.1
prunus_persica Prupe.4G101100_v2.0.a1
pyrus_communis pycom05g24920 pycom05g24930 pycom10g20330
rosa_chinensis RchiOBHm_Chr5g0018041
rosa_laevigata RLG00000032335
rosa_multiflora Rmu_sc0010280.1_g000014
rosa_roxburghii Rroxscaffold_1G00059580
rosa_rugosa Rorug05G0040000
rosa_samantha Rh5AG132800 Rh5BG131100 Rh5BG131300 Rh5CG143300 Rh5DG133000
rosa_wichuraiana Rw5G011560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1473
AccII CGCG 1 cut(s) 243
AciI CCGC 8 cut(s) 248, 348, 354, 731, 1011, 1014, 1134, 1346
AclI AACGTT 1 cut(s) 90
AcsI RAATTY 1 cut(s) 1163
AcvI CACGTG 1 cut(s) 135
AfaI GTAC 5 cut(s) 76, 236, 304, 369, 794
AfiI CCNNNNNNNGG 6 cut(s) 135, 515, 812, 813, 1075, 1273
AflIII ACRYGT 2 cut(s) 132, 906
AgsI TTSAA 4 cut(s) 1021, 1055, 1149, 1399
AjnI CCWGG 1 cut(s) 696
AloI GAACNNNNNNTCC 2 cut(s) 747, 779
Alw26I GTCTC 3 cut(s) 540, 1030, 1266
AlwNI CAGNNNCTG 2 cut(s) 566, 734
Ama87I CYCGRG 1 cut(s) 635
AoxI GGCC 4 cut(s) 434, 746, 947, 1479
ApoI RAATTY 1 cut(s) 1163
AseI ATTAAT 1 cut(s) 597
Asp700I GAANNNNTTC 2 cut(s) 762, 1144
AspA2I CCTAGG 1 cut(s) 539
AspLEI GCGC 4 cut(s) 243, 727, 1007, 1384
AsuC2I CCSGG 2 cut(s) 646, 675
AvaI CYCGRG 1 cut(s) 635
AvrII CCTAGG 1 cut(s) 539
AxyI CCTNAGG 1 cut(s) 1272
BaeI ACNNNNGTAYC 2 cut(s) 476, 509
BamHI GGATCC 1 cut(s) 898
BauI CACGAG 1 cut(s) 309
BbrPI CACGTG 1 cut(s) 135
BccI CCATC 9 cut(s) 25, 154, 178, 399, 815, 948, 1000, 1240, 1275
BceAI ACGGC 2 cut(s) 481, 1018
BcgI CGANNNNNNTGC 2 cut(s) 513, 547
BciT130I CCWGG 1 cut(s) 698
BclI TGATCA 2 cut(s) 109, 361
BcnI CCSGG 2 cut(s) 646, 675
BcoDI GTCTC 3 cut(s) 540, 1030, 1266
BfaI CTAG 5 cut(s) 27, 263, 540, 968, 1065
BfmI CTRYAG 2 cut(s) 461, 838
BfoI RGCGCY 3 cut(s) 728, 1008, 1385
BglII AGATCT 2 cut(s) 465, 654
BlnI CCTAGG 1 cut(s) 539
Bme1390I CCNGG 3 cut(s) 646, 675, 698
BmeT110I CYCGRG 1 cut(s) 635
BmiI GGNNCC 1 cut(s) 900
BmrFI CCNGG 3 cut(s) 646, 675, 698
BmrI ACTGGG 1 cut(s) 291
BmsI GCATC 4 cut(s) 391, 447, 639, 1366
BmuI ACTGGG 1 cut(s) 291
BpmI CTGGAG 2 cut(s) 262, 774
Bpu10I CCTNAGC 1 cut(s) 204
BpuMI CCSGG 2 cut(s) 646, 675
Bsa29I ATCGAT 1 cut(s) 1424
BsaAI YACGTR 1 cut(s) 135
BsaBI GATNNNNATC 4 cut(s) 420, 653, 1200, 1508
BsaI GGTCTC 1 cut(s) 1030
BsaJI CCNNGG 8 cut(s) 186, 539, 573, 665, 674, 827, 944, 1371
BsaXI ACNNNNNCTCC 2 cut(s) 747, 777
Bsc4I CCNNNNNNNGG 6 cut(s) 135, 515, 812, 813, 1075, 1273
Bse118I RCCGGY 1 cut(s) 508
Bse1I ACTGG 3 cut(s) 297, 757, 1258
Bse21I CCTNAGG 1 cut(s) 1272
Bse8I GATNNNNATC 4 cut(s) 420, 653, 1200, 1508
BseBI CCWGG 1 cut(s) 698
BseCI ATCGAT 1 cut(s) 1424
BseDI CCNNGG 8 cut(s) 186, 539, 573, 665, 674, 827, 944, 1371
BseGI GGATG 8 cut(s) 36, 154, 654, 940, 992, 1267, 1354, 1381
BseJI GATNNNNATC 4 cut(s) 420, 653, 1200, 1508
BseLI CCNNNNNNNGG 6 cut(s) 135, 515, 812, 813, 1075, 1273
BseMII CTCAG 3 cut(s) 968, 1263, 1356
BseNI ACTGG 3 cut(s) 297, 757, 1258
BseYI CCCAGC 2 cut(s) 865, 987
BsgI GTGCAG 1 cut(s) 25
Bsh1236I CGCG 1 cut(s) 243
BshFI GGCC 4 cut(s) 436, 748, 949, 1481
BshVI ATCGAT 1 cut(s) 1424
BsiHKCI CYCGRG 1 cut(s) 635
BsiSI CCGG 4 cut(s) 509, 645, 674, 892
BslI CCNNNNNNNGG 6 cut(s) 135, 515, 812, 813, 1075, 1273
BsmAI GTCTC 3 cut(s) 540, 1030, 1266
BsmBI CGTCTC 1 cut(s) 540
BsmI GAATGC 1 cut(s) 717
BsnI GGCC 4 cut(s) 436, 748, 949, 1481
Bso31I GGTCTC 1 cut(s) 1030
BsoBI CYCGRG 1 cut(s) 635
Bsp1407I TGTACA 1 cut(s) 302
Bsp19I CCATGG 3 cut(s) 186, 573, 944
BspACI CCGC 8 cut(s) 248, 348, 354, 731, 1011, 1014, 1134, 1346
BspANI GGCC 4 cut(s) 436, 748, 949, 1481
BspCNI CTCAG 3 cut(s) 967, 1264, 1355
BspDI ATCGAT 1 cut(s) 1424
BspFNI CGCG 1 cut(s) 243
BspHI TCATGA 1 cut(s) 358
BspLI GGNNCC 1 cut(s) 900
BspQI GCTCTTC 1 cut(s) 686
BspTNI GGTCTC 1 cut(s) 1030
BsrFI RCCGGY 1 cut(s) 508
BsrGI TGTACA 1 cut(s) 302
BsrI ACTGG 3 cut(s) 297, 757, 1258
BssAI RCCGGY 1 cut(s) 508
BssECI CCNNGG 8 cut(s) 186, 539, 573, 665, 674, 827, 944, 1371
BssSI CACGAG 1 cut(s) 309
BssT1I CCWWGG 6 cut(s) 186, 539, 573, 827, 944, 1371
Bst2BI CACGAG 1 cut(s) 309
Bst2UI CCWGG 1 cut(s) 698
Bst4CI ACNGT 3 cut(s) 797, 1126, 1153
Bst6I CTCTTC 2 cut(s) 686, 1056
BstAUI TGTACA 1 cut(s) 302
BstBAI YACGTR 1 cut(s) 135
BstC8I GCNNGC 5 cut(s) 583, 632, 729, 746, 973
BstDEI CTNAG 9 cut(s) 65, 204, 371, 954, 1213, 1272, 1342, 1387, 1460
BstDSI CCRYGG 4 cut(s) 186, 573, 665, 944
BstEII GGTNACC 1 cut(s) 816
BstF5I GGATG 8 cut(s) 36, 154, 654, 940, 992, 1267, 1354, 1381
BstFNI CGCG 1 cut(s) 243
BstH2I RGCGCY 3 cut(s) 728, 1008, 1385
BstHHI GCGC 4 cut(s) 243, 727, 1007, 1384
BstMAI GTCTC 3 cut(s) 540, 1030, 1266
BstMWI GCNNNNNNNGC 9 cut(s) 247, 288, 578, 731, 1013, 1142, 1189, 1246, 1376
BstNI CCWGG 1 cut(s) 698
BstNSI RCATGY 2 cut(s) 241, 910
BstPI GGTNACC 1 cut(s) 816
BstSCI CCNGG 3 cut(s) 644, 673, 696
BstSFI CTRYAG 2 cut(s) 461, 838
BstUI CGCG 1 cut(s) 243
BstX2I RGATCY 6 cut(s) 421, 465, 654, 898, 983, 1390
BstXI CCANNNNNNTGG 1 cut(s) 398
BstYI RGATCY 6 cut(s) 421, 465, 654, 898, 983, 1390
Bsu15I ATCGAT 1 cut(s) 1424
Bsu36I CCTNAGG 1 cut(s) 1272
BsuRI GGCC 4 cut(s) 436, 748, 949, 1481
BsuTUI ATCGAT 1 cut(s) 1424
BtgI CCRYGG 4 cut(s) 186, 573, 665, 944
BtsCI GGATG 8 cut(s) 36, 154, 654, 940, 992, 1267, 1354, 1381
BtsI GCAGTG 1 cut(s) 531
BtsIMutI CAGTG 2 cut(s) 531, 1158
Cac8I GCNNGC 5 cut(s) 583, 632, 729, 746, 973
CaiI CAGNNNCTG 2 cut(s) 566, 734
CciI TCATGA 1 cut(s) 358
CfoI GCGC 4 cut(s) 243, 727, 1007, 1384
Cfr10I RCCGGY 1 cut(s) 508
ClaI ATCGAT 1 cut(s) 1424
Csp6I GTAC 5 cut(s) 75, 235, 303, 368, 793
CspCI CAANNNNNGTGG 2 cut(s) 690, 725
CviQI GTAC 5 cut(s) 75, 235, 303, 368, 793
DdeI CTNAG 9 cut(s) 65, 204, 371, 954, 1213, 1272, 1342, 1387, 1460
Eam1104I CTCTTC 2 cut(s) 686, 1056
EarI CTCTTC 2 cut(s) 686, 1056
Eco130I CCWWGG 6 cut(s) 186, 539, 573, 827, 944, 1371
Eco147I AGGCCT 2 cut(s) 436, 748
Eco31I GGTCTC 1 cut(s) 1030
Eco32I GATATC 1 cut(s) 1506
Eco72I CACGTG 1 cut(s) 135
Eco81I CCTNAGG 1 cut(s) 1272
Eco88I CYCGRG 1 cut(s) 635
Eco91I GGTNACC 1 cut(s) 816
EcoO65I GGTNACC 1 cut(s) 816
EcoRII CCWGG 1 cut(s) 696
EcoRV GATATC 1 cut(s) 1506
EcoT14I CCWWGG 6 cut(s) 186, 539, 573, 827, 944, 1371
ErhI CCWWGG 6 cut(s) 186, 539, 573, 827, 944, 1371
Esp3I CGTCTC 1 cut(s) 540
FalI AAGNNNNNCTT 2 cut(s) 197, 229
FbaI TGATCA 2 cut(s) 109, 361
FokI GGATG 8 cut(s) 43, 141, 661, 927, 979, 1254, 1361, 1388
FspBI CTAG 5 cut(s) 27, 263, 540, 968, 1065
GlaI GCGC 4 cut(s) 242, 726, 1006, 1383
GsaI CCCAGC 2 cut(s) 869, 991
GsuI CTGGAG 2 cut(s) 262, 774
HaeII RGCGCY 3 cut(s) 728, 1008, 1385
HaeIII GGCC 4 cut(s) 436, 748, 949, 1481
HapII CCGG 4 cut(s) 509, 645, 674, 892
HhaI GCGC 4 cut(s) 243, 727, 1007, 1384
Hin6I GCGC 4 cut(s) 241, 725, 1005, 1382
HinP1I GCGC 4 cut(s) 241, 725, 1005, 1382
HinfI GANTC 3 cut(s) 503, 563, 670
HpaII CCGG 4 cut(s) 509, 645, 674, 892
Hpy188I TCNGA 6 cut(s) 109, 151, 772, 801, 931, 957
Hpy188III TCNNGA 5 cut(s) 116, 359, 637, 1271, 1419
Hpy99I CGWCG 2 cut(s) 235, 535
HpyAV CCTTC 5 cut(s) 314, 681, 700, 1015, 1150
HpyCH4III ACNGT 3 cut(s) 797, 1126, 1153
HpyCH4IV ACGT 2 cut(s) 90, 134
HpyCH4V TGCA 8 cut(s) 6, 441, 479, 581, 652, 717, 737, 1379
HpyF10VI GCNNNNNNNGC 9 cut(s) 247, 288, 578, 731, 1013, 1142, 1189, 1246, 1376
HpyF3I CTNAG 9 cut(s) 65, 204, 371, 954, 1213, 1272, 1342, 1387, 1460
HpySE526I ACGT 2 cut(s) 90, 134
HspAI GCGC 4 cut(s) 241, 725, 1005, 1382
Ksp22I TGATCA 2 cut(s) 109, 361
LguI GCTCTTC 1 cut(s) 686
LmnI GCTCC 2 cut(s) 281, 521
LweI GCATC 4 cut(s) 391, 447, 639, 1366
MaeI CTAG 5 cut(s) 27, 263, 540, 968, 1065
MaeII ACGT 2 cut(s) 90, 134
MaeIII GTNAC 2 cut(s) 325, 816
MfeI CAATTG 1 cut(s) 1033
MflI RGATCY 6 cut(s) 421, 465, 654, 898, 983, 1390
MluCI AATT 7 cut(s) 21, 37, 1033, 1163, 1184, 1299, 1411
MmeI TCCRAC 2 cut(s) 1145, 1278
MnlI CCTC 9 cut(s) 310, 426, 565, 1059, 1162, 1267, 1297, 1331, 1457
MroXI GAANNNNTTC 2 cut(s) 762, 1144
MseI TTAA 3 cut(s) 53, 255, 597
MslI CAYNNNNRTG 3 cut(s) 363, 396, 647
MspA1I CMGCKG 3 cut(s) 731, 1136, 1346
MspI CCGG 4 cut(s) 509, 645, 674, 892
MspR9I CCNGG 3 cut(s) 646, 675, 698
MunI CAATTG 1 cut(s) 1033
Mva1269I GAATGC 1 cut(s) 717
MvaI CCWGG 1 cut(s) 698
MvnI CGCG 1 cut(s) 243
MwoI GCNNNNNNNGC 9 cut(s) 247, 288, 578, 731, 1013, 1142, 1189, 1246, 1376
NciI CCSGG 2 cut(s) 646, 675
NcoI CCATGG 3 cut(s) 186, 573, 944
NlaIV GGNNCC 1 cut(s) 900
NmuCI GTSAC 2 cut(s) 325, 816
NspI RCATGY 2 cut(s) 241, 910
PagI TCATGA 1 cut(s) 358
PceI AGGCCT 2 cut(s) 436, 748
PciI ACATGT 1 cut(s) 906
PciSI GCTCTTC 1 cut(s) 686
PctI GAATGC 1 cut(s) 717
PdmI GAANNNNTTC 2 cut(s) 762, 1144
PfeI GAWTC 3 cut(s) 503, 563, 670
PmaCI CACGTG 1 cut(s) 135
PmlI CACGTG 1 cut(s) 135
Ppu21I YACGTR 1 cut(s) 135
PscI ACATGT 1 cut(s) 906
PshBI ATTAAT 1 cut(s) 597
PsiI TTATAA 1 cut(s) 1473
Psp1406I AACGTT 1 cut(s) 90
Psp6I CCWGG 1 cut(s) 696
PspCI CACGTG 1 cut(s) 135
PspEI GGTNACC 1 cut(s) 816
PspFI CCCAGC 2 cut(s) 865, 987
PspGI CCWGG 1 cut(s) 696
PspN4I GGNNCC 1 cut(s) 900
PstNI CAGNNNCTG 2 cut(s) 566, 734
PsuI RGATCY 6 cut(s) 421, 465, 654, 898, 983, 1390
RsaI GTAC 5 cut(s) 76, 236, 304, 369, 794
RsaNI GTAC 5 cut(s) 75, 235, 303, 368, 793
RseI CAYNNNNRTG 3 cut(s) 363, 396, 647
SapI GCTCTTC 1 cut(s) 686
SaqAI TTAA 3 cut(s) 53, 255, 597
ScrFI CCNGG 3 cut(s) 646, 675, 698
SfaNI GCATC 4 cut(s) 391, 447, 639, 1366
SfcI CTRYAG 2 cut(s) 461, 838
SgrAI CRCCGGYG 1 cut(s) 508
SmiMI CAYNNNNRTG 3 cut(s) 363, 396, 647
Sse9I AATT 7 cut(s) 21, 37, 1033, 1163, 1184, 1299, 1411
SseBI AGGCCT 2 cut(s) 436, 748
SsiI CCGC 8 cut(s) 248, 348, 354, 731, 1011, 1014, 1134, 1346
SspMI CTAG 5 cut(s) 27, 263, 540, 968, 1065
StuI AGGCCT 2 cut(s) 436, 748
StyD4I CCNGG 3 cut(s) 644, 673, 696
StyI CCWWGG 6 cut(s) 186, 539, 573, 827, 944, 1371
TaaI ACNGT 3 cut(s) 797, 1126, 1153
TaiI ACGT 2 cut(s) 93, 137
TaqI TCGA 1 cut(s) 1424
TasI AATT 7 cut(s) 21, 37, 1033, 1163, 1184, 1299, 1411
TatI WGTACW 2 cut(s) 302, 367
TauI GCSGC 3 cut(s) 734, 1013, 1016
TfiI GAWTC 3 cut(s) 503, 563, 670
Tru1I TTAA 3 cut(s) 53, 255, 597
Tru9I TTAA 3 cut(s) 53, 255, 597
TscAI CASTG 2 cut(s) 531, 1158
TseFI GTSAC 2 cut(s) 325, 816
Tsp45I GTSAC 2 cut(s) 325, 816
TspDTI ATGAA 6 cut(s) 4, 168, 432, 1190, 1436, 1512
TspGWI ACGGA 4 cut(s) 93, 682, 756, 911
TspRI CASTG 2 cut(s) 531, 1158
VspI ATTAAT 1 cut(s) 597
XapI RAATTY 1 cut(s) 1163
XceI RCATGY 2 cut(s) 241, 910
XcmI CCANNNNNNNNNTGG 1 cut(s) 398
XmaJI CCTAGG 1 cut(s) 539
XmnI GAANNNNTTC 2 cut(s) 762, 1144
XspI CTAG 5 cut(s) 27, 263, 540, 968, 1065
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.