pycom10g20890

Ferredoxin-NADP reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0006772
Physical Location & Seq
Reverse (-)
23643170 .. 23645206
2037 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1137 bp
ATGTCTCATTTGGCTCTCTCTCAGGGTTCACTGACTGTGACTGTTCCGCTTGGAAGTGATTTCTCCCTCCGAAGATCTGTGTTCAAGGCACGAAGTGTTAACTTTAATGATAAATCATGGGCTCCTATACCTTTGGACTTCAAAACGAAAAAGGGACGGCTGAGAAATCAGCGCATTCTATGCATGTCAGTGCAACAAGCAAGTGCGCCTAAAGTTAATGTAGCTCCTCTACAACTTGAGGATGCTAATGAGCCCCCATTAAATACATACAAGCCTAAGGAGCCTTATACTGCAACCATTCTTTCTGTTGAGAGGATTGTCGGCCCAAATGCTCCTGGAGAGACTTGCCATATTGTGATTGATCATGGTGGTAATGTGCCTTACTGGGAAGGGCAAAGTTATGGTGTCATCCCTCCGGGGGAAAACCCAAAGAAACCTGGGGCTCCGCACAATGTTCGCCTTTACTCAATTGCATCGACAAGGTATGGAGACTATTTTGATGGCAAGACAACTAGTTTGTGTGTTCGTCGTGCTGTCTATTACGACCCTGAGACAGGAAAGGAGGATCGATCCAAGAATGGTGTATGCAGCAACTTTTTATGCAACTCCAAACCTGGTGATAAAATTAAGATCACAGGGCCTGCTGGCAAGATCATGCTTTTGCCTGAAGACAACCCAAATGCCACCCACATTATGATTGCAACTGGCACTGGTATTGCCCCGTACAGAGGTTACCTCCGTCGCATGTTCATGGAATCTGTTCCCAAATTCAAGTTTGGCGGCCTTGCATGGCTATTCCTTGGTGTGGCCAACACTGATAGTCTCCTATATGATGAAGAATTCACCAAGTATCTCAAGGACTACCCCGATAATTTCCGGTACGACATAGCACTTAGCAGAGAACAGAAGAACCAGAGAGGAGGGAAGATGTATGTTCAGGATAAAATCGAGGAGTACAGTGACGAGATCTTTAAGCTATTGGACAACGGGGCCCACATATACTTCTGCGGGCTCAAGGGAATGATGCCCGGGATTCAAGAAACCTTGAAGAGGGTTGCGGTTGAAAGAGGGGAGAGCTGGGAGGAGAAGCTTTCACAGCTGAAAAAGAACAAGCAATGGCATGTGGAAGTCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00195 ko01100 map00195 map01100
Pfam Domains
Protein Families
NAD_binding_1

Protein Analysis

379

Amino Acids

42.58

Weight (kDa)

9.03

Isoelectric Point (pI)

41.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_1 PF00175 232 - 346 2.9e-25 Oxidoreductase NAD-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1131
AciI CCGC 5 cut(s) 47, 446, 780, 1008, 1058
AclWI GGATC 2 cut(s) 564, 573
AcoI YGGCCR 1 cut(s) 807
AcsI RAATTY 2 cut(s) 767, 839
AcuI CTGAAG 1 cut(s) 687
AdeI CACNNNGTG 1 cut(s) 95
AfaI GTAC 3 cut(s) 725, 881, 956
AfiI CCNNNNNNNGG 5 cut(s) 418, 554, 728, 805, 1050
AgsI TTSAA 6 cut(s) 85, 142, 772, 1037, 1048, 1064
AhlI ACTAGT 1 cut(s) 512
AjnI CCWGG 3 cut(s) 334, 436, 613
AluBI AGCT 5 cut(s) 224, 976, 1077, 1090, 1099
AluI AGCT 5 cut(s) 224, 976, 1077, 1090, 1099
Alw26I GTCTC 5 cut(s) 9, 335, 483, 545, 827
AlwI GGATC 2 cut(s) 564, 573
AlwNI CAGNNNCTG 1 cut(s) 641
Ama87I CYCGRG 1 cut(s) 1028
AoxI GGCC 5 cut(s) 322, 638, 781, 807, 990
ApaI GGGCCC 1 cut(s) 994
ApeKI GCWGC 1 cut(s) 588
ApoI RAATTY 2 cut(s) 767, 839
ArsI GACNNNNNNTTYG 2 cut(s) 499, 531
Asp700I GAANNNNTTC 1 cut(s) 759
AspLEI GCGC 2 cut(s) 174, 208
AspS9I GGNCC 4 cut(s) 323, 638, 990, 991
AsuC2I CCSGG 3 cut(s) 417, 1029, 1030
AsuHPI GGTGA 2 cut(s) 629, 835
AvaI CYCGRG 1 cut(s) 1028
AxyI CCTNAGG 1 cut(s) 276
BaeGI GKGCMC 1 cut(s) 994
BalI TGGCCA 1 cut(s) 809
BanII GRGCYC 5 cut(s) 124, 255, 445, 994, 1014
BbsI GAAGAC 1 cut(s) 675
BbvI GCAGC 1 cut(s) 600
BccI CCATC 1 cut(s) 494
BceAI ACGGC 1 cut(s) 173
BcgI CGANNNNNNTGC 2 cut(s) 437, 471
BciT130I CCWGG 3 cut(s) 336, 438, 615
BclI TGATCA 1 cut(s) 361
BcnI CCSGG 3 cut(s) 417, 1029, 1030
BcoDI GTCTC 5 cut(s) 9, 335, 483, 545, 827
BcuI ACTAGT 1 cut(s) 512
BfaI CTAG 1 cut(s) 513
BglII AGATCT 2 cut(s) 74, 966
BisI GCNGC 2 cut(s) 589, 781
BlsI GCNGC 2 cut(s) 590, 782
Bme1390I CCNGG 6 cut(s) 336, 417, 438, 615, 1029, 1030
BmeT110I CYCGRG 1 cut(s) 1028
BmgT120I GGNCC 4 cut(s) 323, 638, 990, 991
BmiI GGNNCC 5 cut(s) 123, 282, 444, 991, 992
BmrFI CCNGG 6 cut(s) 336, 417, 438, 615, 1029, 1030
BmrI ACTGGG 1 cut(s) 394
BmsI GCATC 3 cut(s) 232, 482, 1014
BmuI ACTGGG 1 cut(s) 394
BpiI GAAGAC 1 cut(s) 675
BplI GAGNNNNNCTC 2 cut(s) 720, 752
BpmI CTGGAG 1 cut(s) 357
BpuEI CTTGAG 3 cut(s) 257, 839, 998
BpuMI CCSGG 3 cut(s) 417, 1029, 1030
Bsa29I ATCGAT 1 cut(s) 568
BsaJI CCNNGG 4 cut(s) 416, 437, 799, 1028
BsaWI WCCGGW 1 cut(s) 876
Bsc4I CCNNNNNNNGG 5 cut(s) 418, 554, 728, 805, 1050
Bse1I ACTGG 3 cut(s) 389, 709, 715
Bse21I CCTNAGG 1 cut(s) 276
Bse3DI GCAATG 1 cut(s) 1121
BseBI CCWGG 3 cut(s) 336, 438, 615
BseCI ATCGAT 1 cut(s) 568
BseDI CCNNGG 4 cut(s) 416, 437, 799, 1028
BseGI GGATG 2 cut(s) 247, 408
BseLI CCNNNNNNNGG 5 cut(s) 418, 554, 728, 805, 1050
BseMI GCAATG 1 cut(s) 1121
BseMII CTCAG 3 cut(s) 35, 152, 540
BseNI ACTGG 3 cut(s) 389, 709, 715
BseRI GAGGAG 4 cut(s) 216, 933, 965, 1097
BseSI GKGCMC 1 cut(s) 994
BseXI GCAGC 1 cut(s) 600
BseYI CCCAGC 1 cut(s) 1077
BshFI GGCC 5 cut(s) 324, 640, 783, 809, 992
BshVI ATCGAT 1 cut(s) 568
BsiHKCI CYCGRG 1 cut(s) 1028
BsiSI CCGG 3 cut(s) 416, 877, 1029
BslFI GGGAC 1 cut(s) 168
BslI CCNNNNNNNGG 5 cut(s) 418, 554, 728, 805, 1050
BsmAI GTCTC 5 cut(s) 9, 335, 483, 545, 827
BsmFI GGGAC 1 cut(s) 168
BsmI GAATGC 1 cut(s) 174
BsnI GGCC 5 cut(s) 324, 640, 783, 809, 992
BsoBI CYCGRG 1 cut(s) 1028
Bsp120I GGGCCC 1 cut(s) 990
Bsp1286I GDGCHC 5 cut(s) 124, 255, 445, 994, 1014
Bsp143I GATC 7 cut(s) 74, 361, 565, 569, 630, 651, 966
BspACI CCGC 5 cut(s) 47, 446, 780, 1008, 1058
BspANI GGCC 5 cut(s) 324, 640, 783, 809, 992
BspCNI CTCAG 3 cut(s) 34, 153, 541
BspDI ATCGAT 1 cut(s) 568
BspLI GGNNCC 5 cut(s) 123, 282, 444, 991, 992
BspPI GGATC 2 cut(s) 564, 573
BsrDI GCAATG 1 cut(s) 1121
BsrI ACTGG 3 cut(s) 389, 709, 715
BssECI CCNNGG 4 cut(s) 416, 437, 799, 1028
BssMI GATC 7 cut(s) 74, 361, 565, 569, 630, 651, 966
BssT1I CCWWGG 1 cut(s) 799
Bst2UI CCWGG 3 cut(s) 336, 438, 615
Bst4CI ACNGT 3 cut(s) 37, 43, 959
Bst6I CTCTTC 1 cut(s) 1043
BstAPI GCANNNNNTGC 1 cut(s) 180
BstC8I GCNNGC 3 cut(s) 642, 646, 1010
BstDEI CTNAG 5 cut(s) 21, 161, 276, 549, 893
BstEII GGTNACC 1 cut(s) 731
BstENI CCTNNNNNAGG 2 cut(s) 552, 1048
BstF5I GGATG 2 cut(s) 247, 408
BstHHI GCGC 2 cut(s) 174, 208
BstKTI GATC 7 cut(s) 77, 364, 568, 572, 633, 654, 969
BstMAI GTCTC 5 cut(s) 9, 335, 483, 545, 827
BstMBI GATC 7 cut(s) 74, 361, 565, 569, 630, 651, 966
BstMWI GCNNNNNNNGC 3 cut(s) 180, 280, 1096
BstNI CCWGG 3 cut(s) 336, 438, 615
BstNSI RCATGY 3 cut(s) 187, 748, 1124
BstPI GGTNACC 1 cut(s) 731
BstSCI CCNGG 6 cut(s) 334, 415, 436, 613, 1027, 1028
BstSLI GKGCMC 1 cut(s) 994
BstV1I GCAGC 1 cut(s) 600
BstV2I GAAGAC 1 cut(s) 675
BstX2I RGATCY 2 cut(s) 74, 966
BstYI RGATCY 2 cut(s) 74, 966
Bsu15I ATCGAT 1 cut(s) 568
Bsu36I CCTNAGG 1 cut(s) 276
BsuRI GGCC 5 cut(s) 324, 640, 783, 809, 992
BsuTUI ATCGAT 1 cut(s) 568
BtsCI GGATG 2 cut(s) 247, 408
BtsIMutI CAGTG 5 cut(s) 29, 195, 708, 813, 964
Cac8I GCNNGC 3 cut(s) 642, 646, 1010
CaiI CAGNNNCTG 1 cut(s) 641
CfoI GCGC 2 cut(s) 174, 208
Cfr13I GGNCC 4 cut(s) 323, 638, 990, 991
Cfr9I CCCGGG 1 cut(s) 1028
ClaI ATCGAT 1 cut(s) 568
CsiI ACCWGGT 1 cut(s) 613
Csp6I GTAC 3 cut(s) 724, 880, 955
CviAII CATG 8 cut(s) 117, 184, 365, 655, 745, 751, 789, 1121
CviQI GTAC 3 cut(s) 724, 880, 955
DdeI CTNAG 5 cut(s) 21, 161, 276, 549, 893
DpnI GATC 7 cut(s) 76, 363, 567, 571, 632, 653, 968
DpnII GATC 7 cut(s) 74, 361, 565, 569, 630, 651, 966
DraIII CACNNNGTG 1 cut(s) 95
EaeI YGGCCR 1 cut(s) 807
Eam1104I CTCTTC 1 cut(s) 1043
EarI CTCTTC 1 cut(s) 1043
Eco130I CCWWGG 1 cut(s) 799
Eco24I GRGCYC 5 cut(s) 124, 255, 445, 994, 1014
Eco57I CTGAAG 1 cut(s) 687
Eco81I CCTNAGG 1 cut(s) 276
Eco88I CYCGRG 1 cut(s) 1028
Eco91I GGTNACC 1 cut(s) 731
EcoNI CCTNNNNNAGG 2 cut(s) 552, 1048
EcoO109I RGGNCCY 2 cut(s) 638, 990
EcoO65I GGTNACC 1 cut(s) 731
EcoRI GAATTC 1 cut(s) 839
EcoRII CCWGG 3 cut(s) 334, 436, 613
EcoT14I CCWWGG 1 cut(s) 799
EcoT22I ATGCAT 1 cut(s) 185
EcoT38I GRGCYC 5 cut(s) 124, 255, 445, 994, 1014
ErhI CCWWGG 1 cut(s) 799
FaeI CATG 8 cut(s) 120, 187, 368, 658, 748, 754, 792, 1124
FaqI GGGAC 1 cut(s) 168
FatI CATG 8 cut(s) 116, 183, 364, 654, 744, 750, 788, 1120
FauI CCCGC 1 cut(s) 1001
FbaI TGATCA 1 cut(s) 361
FblI GTMKAC 1 cut(s) 1131
Fnu4HI GCNGC 2 cut(s) 589, 781
FokI GGATG 2 cut(s) 254, 395
FriOI GRGCYC 5 cut(s) 124, 255, 445, 994, 1014
Fsp4HI GCNGC 2 cut(s) 589, 781
FspBI CTAG 1 cut(s) 513
GlaI GCGC 2 cut(s) 173, 207
GluI GCNGC 2 cut(s) 589, 781
GsaI CCCAGC 1 cut(s) 1081
GsuI CTGGAG 1 cut(s) 357
HaeIII GGCC 5 cut(s) 324, 640, 783, 809, 992
HapII CCGG 3 cut(s) 416, 877, 1029
HhaI GCGC 2 cut(s) 174, 208
Hin1II CATG 8 cut(s) 120, 187, 368, 658, 748, 754, 792, 1124
Hin6I GCGC 2 cut(s) 172, 206
HinP1I GCGC 2 cut(s) 172, 206
HincII GTYRAC 1 cut(s) 100
HindII GTYRAC 1 cut(s) 100
HindIII AAGCTT 1 cut(s) 1088
HinfI GANTC 2 cut(s) 755, 1033
HpaI GTTAAC 1 cut(s) 100
HpaII CCGG 3 cut(s) 416, 877, 1029
HphI GGTGA 2 cut(s) 629, 835
Hpy166II GTNNAC 3 cut(s) 29, 100, 1132
Hpy188I TCNGA 1 cut(s) 71
Hpy188III TCNNGA 2 cut(s) 938, 1037
Hpy8I GTNNAC 3 cut(s) 29, 100, 1132
Hpy99I CGWCG 2 cut(s) 531, 744
HpyAV CCTTC 1 cut(s) 383
HpyCH4III ACNGT 3 cut(s) 37, 43, 959
HpyCH4V TGCA 8 cut(s) 183, 193, 293, 473, 588, 603, 701, 788
HpyF10VI GCNNNNNNNGC 3 cut(s) 180, 280, 1096
HpyF3I CTNAG 5 cut(s) 21, 161, 276, 549, 893
Hsp92II CATG 8 cut(s) 120, 187, 368, 658, 748, 754, 792, 1124
HspAI GCGC 2 cut(s) 172, 206
Ksp22I TGATCA 1 cut(s) 361
KspAI GTTAAC 1 cut(s) 100
Kzo9I GATC 7 cut(s) 74, 361, 565, 569, 630, 651, 966
LmnI GCTCC 5 cut(s) 127, 229, 280, 337, 448
Lsp1109I GCAGC 1 cut(s) 600
LweI GCATC 3 cut(s) 232, 482, 1014
MabI ACCWGGT 1 cut(s) 613
MaeI CTAG 1 cut(s) 513
MaeIII GTNAC 3 cut(s) 37, 731, 959
MalI GATC 7 cut(s) 76, 363, 567, 571, 632, 653, 968
MboI GATC 7 cut(s) 74, 361, 565, 569, 630, 651, 966
MboII GAAGA 6 cut(s) 84, 680, 848, 919, 937, 1060
MfeI CAATTG 1 cut(s) 468
MflI RGATCY 2 cut(s) 74, 966
MhlI GDGCHC 5 cut(s) 124, 255, 445, 994, 1014
MlsI TGGCCA 1 cut(s) 809
MluCI AATT 5 cut(s) 468, 624, 767, 839, 871
MluNI TGGCCA 1 cut(s) 809
Mox20I TGGCCA 1 cut(s) 809
Mph1103I ATGCAT 1 cut(s) 185
MroXI GAANNNNTTC 1 cut(s) 759
MscI TGGCCA 1 cut(s) 809
MseI TTAA 6 cut(s) 99, 105, 216, 260, 627, 972
MslI CAYNNNNRTG 2 cut(s) 188, 749
Msp20I TGGCCA 1 cut(s) 809
MspA1I CMGCKG 1 cut(s) 1099
MspI CCGG 3 cut(s) 416, 877, 1029
MspR9I CCNGG 6 cut(s) 336, 417, 438, 615, 1029, 1030
MunI CAATTG 1 cut(s) 468
Mva1269I GAATGC 1 cut(s) 174
MvaI CCWGG 3 cut(s) 336, 438, 615
MwoI GCNNNNNNNGC 3 cut(s) 180, 280, 1096
NciI CCSGG 3 cut(s) 417, 1029, 1030
NdeII GATC 7 cut(s) 74, 361, 565, 569, 630, 651, 966
NlaIII CATG 8 cut(s) 120, 187, 368, 658, 748, 754, 792, 1124
NlaIV GGNNCC 5 cut(s) 123, 282, 444, 991, 992
NmuCI GTSAC 2 cut(s) 37, 959
NsiI ATGCAT 1 cut(s) 185
NspI RCATGY 3 cut(s) 187, 748, 1124
PctI GAATGC 1 cut(s) 174
PdmI GAANNNNTTC 1 cut(s) 759
PfeI GAWTC 2 cut(s) 755, 1033
PfoI TCCNGGA 1 cut(s) 334
PkrI GCNGC 2 cut(s) 590, 782
Psp6I CCWGG 3 cut(s) 334, 436, 613
PspEI GGTNACC 1 cut(s) 731
PspFI CCCAGC 1 cut(s) 1077
PspGI CCWGG 3 cut(s) 334, 436, 613
PspN4I GGNNCC 5 cut(s) 123, 282, 444, 991, 992
PspOMI GGGCCC 1 cut(s) 990
PspPI GGNCC 4 cut(s) 323, 638, 990, 991
PstNI CAGNNNCTG 1 cut(s) 641
PsuI RGATCY 2 cut(s) 74, 966
PvuII CAGCTG 1 cut(s) 1099
RsaI GTAC 3 cut(s) 725, 881, 956
RsaNI GTAC 3 cut(s) 724, 880, 955
RseI CAYNNNNRTG 2 cut(s) 188, 749
SaqAI TTAA 6 cut(s) 99, 105, 216, 260, 627, 972
SatI GCNGC 2 cut(s) 589, 781
Sau3AI GATC 7 cut(s) 74, 361, 565, 569, 630, 651, 966
Sau96I GGNCC 4 cut(s) 323, 638, 990, 991
ScrFI CCNGG 6 cut(s) 336, 417, 438, 615, 1029, 1030
SduI GDGCHC 5 cut(s) 124, 255, 445, 994, 1014
SexAI ACCWGGT 1 cut(s) 613
SfaNI GCATC 3 cut(s) 232, 482, 1014
SmaI CCCGGG 1 cut(s) 1030
SmiMI CAYNNNNRTG 2 cut(s) 188, 749
SmlI CTYRAG 3 cut(s) 236, 854, 1013
SmoI CTYRAG 3 cut(s) 236, 854, 1013
SpeI ACTAGT 1 cut(s) 512
Sse9I AATT 5 cut(s) 468, 624, 767, 839, 871
SsiI CCGC 5 cut(s) 47, 446, 780, 1008, 1058
SspMI CTAG 1 cut(s) 513
StyD4I CCNGG 6 cut(s) 334, 415, 436, 613, 1027, 1028
StyI CCWWGG 1 cut(s) 799
TaaI ACNGT 3 cut(s) 37, 43, 959
TaqI TCGA 3 cut(s) 476, 568, 948
TasI AATT 5 cut(s) 468, 624, 767, 839, 871
TatI WGTACW 1 cut(s) 954
TauI GCSGC 1 cut(s) 783
TfiI GAWTC 2 cut(s) 755, 1033
Tru1I TTAA 6 cut(s) 99, 105, 216, 260, 627, 972
Tru9I TTAA 6 cut(s) 99, 105, 216, 260, 627, 972
TscAI CASTG 5 cut(s) 36, 195, 715, 820, 964
TseFI GTSAC 2 cut(s) 37, 959
TseI GCWGC 1 cut(s) 588
Tsp45I GTSAC 2 cut(s) 37, 959
TspDTI ATGAA 2 cut(s) 739, 849
TspGWI ACGGA 1 cut(s) 728
TspMI CCCGGG 1 cut(s) 1028
TspRI CASTG 5 cut(s) 36, 195, 715, 820, 964
XagI CCTNNNNNAGG 2 cut(s) 552, 1048
XapI RAATTY 2 cut(s) 767, 839
XceI RCATGY 3 cut(s) 187, 748, 1124
XmaI CCCGGG 1 cut(s) 1028
XmiI GTMKAC 1 cut(s) 1131
XmnI GAANNNNTTC 1 cut(s) 759
XspI CTAG 1 cut(s) 513
Zsp2I ATGCAT 1 cut(s) 185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.