pycom10g22420

AWPM-19-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0009983
Physical Location & Seq
Forward (+)
24780883 .. 24781682
800 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 480 bp
ATGGCCAACCAGCAGTTAAAACCCGTTGCCGGACTGCTTTTGGCTCTCAATTTCTGTATGTATGTCATCGTTTTGGGCATTGGAGGTTGGGCAATGAACCGAGCAATCGACCACGGTTTTGTTATTGGTCCTGGTTTTGATCTCCCGGCACATTTTTCGCCAATATATTTCCCCATGGGCAATGCTGCTACCGGGTTCTTTGTGACATTCGCTCTGCTCGCTGGAGTTGTTGGCGTAGCTTCAGCTATTTCCGGTATCAACCATCTTCGATCTTGGACCCATGAGAGCTTGCCGTCTGCAGCCACAGCTGCCACCATTGCCTGGACCCTCACCCTACTTGCCATGAGCTTCGGTTGCAAGGAGATCGAGCTTCGGATCAGAAATTCCCGATTGAGAACAATGGAGGCCTTCTTGATCATCCTTTCGGTGACGCAGCTTCTGTATTTAGCAGTCATTCATACCGTTGTATCGAGGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AWPM-19

Protein Analysis

160

Amino Acids

17.2

Weight (kDa)

9.58

Isoelectric Point (pI)

38.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AWPM-19 PF05512 16 - 154 3.6e-64 AWPM-19-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 321
AclWI GGATC 1 cut(s) 383
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 382
AcuI CTGAAG 1 cut(s) 225
AfiI CCNNNNNNNGG 2 cut(s) 29, 321
AjnI CCWGG 2 cut(s) 130, 320
AluBI AGCT 7 cut(s) 239, 245, 288, 308, 348, 370, 436
AluI AGCT 7 cut(s) 239, 245, 288, 308, 348, 370, 436
AlwI GGATC 1 cut(s) 383
AlwNI CAGNNNCTG 1 cut(s) 439
AoxI GGCC 2 cut(s) 3, 405
ApeKI GCWGC 4 cut(s) 185, 299, 308, 433
ApoI RAATTY 1 cut(s) 382
ArsI GACNNNNNNTTYG 2 cut(s) 101, 133
AspS9I GGNCC 3 cut(s) 128, 276, 324
AsuC2I CCSGG 2 cut(s) 146, 193
AsuHPI GGTGA 2 cut(s) 322, 439
AvaII GGWCC 3 cut(s) 128, 276, 324
BaeI ACNNNNGTAYC 1 cut(s) 450
BalI TGGCCA 1 cut(s) 5
BbvI GCAGC 4 cut(s) 172, 295, 311, 445
BccI CCATC 1 cut(s) 270
BceAI ACGGC 1 cut(s) 277
BcgI CGANNNNNNTGC 4 cut(s) 138, 172, 346, 380
BciT130I CCWGG 2 cut(s) 132, 322
BclI TGATCA 1 cut(s) 414
BcnI CCSGG 2 cut(s) 146, 193
BfmI CTRYAG 1 cut(s) 297
BisI GCNGC 4 cut(s) 186, 300, 309, 434
BlsI GCNGC 4 cut(s) 187, 301, 310, 435
Bme1390I CCNGG 4 cut(s) 132, 146, 193, 322
Bme18I GGWCC 3 cut(s) 128, 276, 324
BmgT120I GGNCC 3 cut(s) 128, 276, 324
BmiI GGNNCC 2 cut(s) 278, 326
BmrFI CCNGG 4 cut(s) 132, 146, 193, 322
BpmI CTGGAG 1 cut(s) 243
BpuMI CCSGG 2 cut(s) 146, 193
BsaJI CCNNGG 2 cut(s) 112, 174
BsaWI WCCGGW 1 cut(s) 251
Bsc4I CCNNNNNNNGG 2 cut(s) 29, 321
Bse3DI GCAATG 3 cut(s) 99, 187, 315
BseBI CCWGG 2 cut(s) 132, 322
BseDI CCNNGG 2 cut(s) 112, 174
BseGI GGATG 1 cut(s) 417
BseLI CCNNNNNNNGG 2 cut(s) 29, 321
BseMI GCAATG 3 cut(s) 99, 187, 315
BseXI GCAGC 4 cut(s) 172, 295, 311, 445
BshFI GGCC 2 cut(s) 5, 407
BsiSI CCGG 4 cut(s) 30, 146, 192, 252
BslI CCNNNNNNNGG 2 cut(s) 29, 321
BsnI GGCC 2 cut(s) 5, 407
Bsp143I GATC 5 cut(s) 139, 269, 363, 375, 414
Bsp19I CCATGG 1 cut(s) 174
BspANI GGCC 2 cut(s) 5, 407
BspLI GGNNCC 2 cut(s) 278, 326
BspMAI CTGCAG 1 cut(s) 301
BspPI GGATC 1 cut(s) 383
BsrDI GCAATG 3 cut(s) 99, 187, 315
BssECI CCNNGG 2 cut(s) 112, 174
BssMI GATC 5 cut(s) 139, 269, 363, 375, 414
BssT1I CCWWGG 1 cut(s) 174
Bst2UI CCWGG 2 cut(s) 132, 322
Bst4CI ACNGT 2 cut(s) 116, 463
BstC8I GCNNGC 2 cut(s) 219, 290
BstDSI CCRYGG 2 cut(s) 112, 174
BstF5I GGATG 1 cut(s) 417
BstKTI GATC 5 cut(s) 142, 272, 366, 378, 417
BstMBI GATC 5 cut(s) 139, 269, 363, 375, 414
BstMWI GCNNNNNNNGC 5 cut(s) 218, 305, 308, 317, 354
BstNI CCWGG 2 cut(s) 132, 322
BstSCI CCNGG 4 cut(s) 130, 144, 191, 320
BstSFI CTRYAG 1 cut(s) 297
BstV1I GCAGC 4 cut(s) 172, 295, 311, 445
BsuRI GGCC 2 cut(s) 5, 407
BtgI CCRYGG 2 cut(s) 112, 174
BtsCI GGATG 1 cut(s) 417
Cac8I GCNNGC 2 cut(s) 219, 290
CaiI CAGNNNCTG 1 cut(s) 439
Cfr13I GGNCC 3 cut(s) 128, 276, 324
CseI GACGC 1 cut(s) 439
CviAII CATG 3 cut(s) 175, 281, 343
DpnI GATC 5 cut(s) 141, 271, 365, 377, 416
DpnII GATC 5 cut(s) 139, 269, 363, 375, 414
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 1 cut(s) 174
Eco147I AGGCCT 1 cut(s) 407
Eco47I GGWCC 3 cut(s) 128, 276, 324
Eco57I CTGAAG 1 cut(s) 225
EcoRII CCWGG 2 cut(s) 130, 320
EcoT14I CCWWGG 1 cut(s) 174
ErhI CCWWGG 1 cut(s) 174
FaeI CATG 3 cut(s) 178, 284, 346
FaiI YATR 7 cut(s) 59, 63, 166, 176, 282, 344, 459
FatI CATG 3 cut(s) 174, 280, 342
FbaI TGATCA 1 cut(s) 414
Fnu4HI GCNGC 4 cut(s) 186, 300, 309, 434
FokI GGATG 1 cut(s) 404
Fsp4HI GCNGC 4 cut(s) 186, 300, 309, 434
GluI GCNGC 4 cut(s) 186, 300, 309, 434
GsuI CTGGAG 1 cut(s) 243
HaeIII GGCC 2 cut(s) 5, 407
HapII CCGG 4 cut(s) 30, 146, 192, 252
HgaI GACGC 1 cut(s) 439
Hin1II CATG 3 cut(s) 178, 284, 346
HpaII CCGG 4 cut(s) 30, 146, 192, 252
HphI GGTGA 2 cut(s) 322, 439
Hpy188I TCNGA 2 cut(s) 375, 380
Hpy188III TCNNGA 2 cut(s) 387, 412
HpyAV CCTTC 1 cut(s) 418
HpyCH4III ACNGT 2 cut(s) 116, 463
HpyCH4V TGCA 2 cut(s) 299, 357
HpyF10VI GCNNNNNNNGC 5 cut(s) 218, 305, 308, 317, 354
Hsp92II CATG 3 cut(s) 178, 284, 346
Ksp22I TGATCA 1 cut(s) 414
Kzo9I GATC 5 cut(s) 139, 269, 363, 375, 414
Lsp1109I GCAGC 4 cut(s) 172, 295, 311, 445
MaeIII GTNAC 2 cut(s) 202, 427
MalI GATC 5 cut(s) 141, 271, 365, 377, 416
MboI GATC 5 cut(s) 139, 269, 363, 375, 414
MboII GAAGA 1 cut(s) 257
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 49, 382
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 4 cut(s) 77, 338, 397, 465
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 17
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 308
MspI CCGG 4 cut(s) 30, 146, 192, 252
MspR9I CCNGG 4 cut(s) 132, 146, 193, 322
MvaI CCWGG 2 cut(s) 132, 322
MwoI GCNNNNNNNGC 5 cut(s) 218, 305, 308, 317, 354
NciI CCSGG 2 cut(s) 146, 193
NcoI CCATGG 1 cut(s) 174
NdeII GATC 5 cut(s) 139, 269, 363, 375, 414
NlaIII CATG 3 cut(s) 178, 284, 346
NlaIV GGNNCC 2 cut(s) 278, 326
NmuCI GTSAC 2 cut(s) 202, 427
PceI AGGCCT 1 cut(s) 407
PflMI CCANNNNNTGG 1 cut(s) 321
PkrI GCNGC 4 cut(s) 187, 301, 310, 435
Psp6I CCWGG 2 cut(s) 130, 320
PspGI CCWGG 2 cut(s) 130, 320
PspN4I GGNNCC 2 cut(s) 278, 326
PspPI GGNCC 3 cut(s) 128, 276, 324
PstI CTGCAG 1 cut(s) 301
PstNI CAGNNNCTG 1 cut(s) 439
PvuII CAGCTG 1 cut(s) 308
SaqAI TTAA 1 cut(s) 17
SatI GCNGC 4 cut(s) 186, 300, 309, 434
Sau3AI GATC 5 cut(s) 139, 269, 363, 375, 414
Sau96I GGNCC 3 cut(s) 128, 276, 324
ScrFI CCNGG 4 cut(s) 132, 146, 193, 322
SetI ASST 8 cut(s) 88, 241, 247, 290, 310, 350, 372, 438
SfcI CTRYAG 1 cut(s) 297
SinI GGWCC 3 cut(s) 128, 276, 324
Sse9I AATT 2 cut(s) 49, 382
SseBI AGGCCT 1 cut(s) 407
StuI AGGCCT 1 cut(s) 407
StyD4I CCNGG 4 cut(s) 130, 144, 191, 320
StyI CCWWGG 1 cut(s) 174
TaaI ACNGT 2 cut(s) 116, 463
TaqI TCGA 4 cut(s) 108, 268, 366, 470
TasI AATT 2 cut(s) 49, 382
Tru1I TTAA 1 cut(s) 17
Tru9I TTAA 1 cut(s) 17
TseFI GTSAC 2 cut(s) 202, 427
TseI GCWGC 4 cut(s) 185, 299, 308, 433
Tsp45I GTSAC 2 cut(s) 202, 427
TspDTI ATGAA 2 cut(s) 110, 446
Van91I CCANNNNNTGG 1 cut(s) 321
VpaK11BI GGWCC 3 cut(s) 128, 276, 324
XapI RAATTY 1 cut(s) 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.