Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0004775

Physical Location & Seq

Genomic Coordinates Reverse (-)

25564303 .. 25569226

Gene Structure 4924 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1542 bp

ATGACTAGTCTGCTTAACAATTCTCAAGAAATTAATATCGAATTAATACCGACAAGAAGGCAAACCCACCTGTGCCATTTTACTACCCAATTGCCCCGCTCCTTCAATTTGCCGCCGCAACCCCGCCGTCCGTGCAGCTCTCTTTCCCTTCGGCTGCTCACTCACTACGAACTAGCCATGGCGGCAAGGGCTTCCATAAAATTGCTGAGAGACTTTTTGTTCAAAAGGGCATGCGCAGCTCCTGCTGCAAGTGCCAGACATTGTTCTTCTGCTGCTCAAACTGCTCCAAAGATTCCACATTTTTCGAAGAAAGGGAGGTTGTTGACGGCAGCCACCTTAGGCTTAGTTATCGGTGGCGGAGCTTATGTGAGCACTGTGGATGAAGCAACTTTCTGTGGCTGGCTATTCTCTGCTACAAAAATTGTGAATCCATTCTTCGCTCTTCTGGATGCTGAGACAGCTCACAGATTGGCTGTCTCAGCTGCAGCACGTGGTTGGGTACCTAGAGAGAAGAGACCAGATCCATCAAATTTAGGGCTAGAAGTTTGGGGAAGGAAGTTCTCCAACCCTATAGGTCTTGCTGCTGGCTTTGATAAGAATGCTGAAGCTGTTGATGGTTTGCTTGGTTTAGGCTTTGGATTTGTAGAGATTGGCTCTGTAACCCCTGTTCCACAAGAGGGCAATCCAAAGCCTCGTATCTTCAGATTGCGCGAGGAAGTAGCTATCATCAATCGATGTGGCTTTAATAGCGAGGGAATTGTAGCTGTTGCAAAGCGATTGGGTGCTCAGCACGGTAAGAGAAAGTTGGATGAAACTACAAGCTCATCTTCTTCAGGTGAAGAAGTCAAACCTGGAGGCAAAGCTGGCCCTGGCATACTAGGCGTGAATCTTGGGAAGAATAAGACAAGTGAAGATGCTTCTGCAGATTATAGACAAGGGGTTCATACATTGTCCCAGTACGCTGATTACTTGGTGATCAATGTTTCATCACCCAACACCCCTGGGTTACGTATGCTTCAGGGAAGAAAGCAGTTAAAGGACCTTGTGAAGCAGGTTCAAGGTGCTCGTGATGAAATGCAATGGGGTGAGGAGGGGCCTCCTCCATTGCTTGTGAAAATTGCTCCAGATTTGTCTAAAGAAGACCTTGAAGATATTGCTGCAGTTGCCCTTGCCCTTCGCTTGGACGGATTGATTATATCAAATACAACTGTTTCAAGACCAGAATCTGTTAGTAAACACCCGTTGGCTTCTGAAGCTGGTGGCTTGAGTGGGAAGCCACTTTTCGATATGTCTACCAATGTCATAAAGGAGATGTACATTTTGACAAGGGGAAAGATTCCCTTAATCGGGTGTGGGGGCATCAGCAGTGGCGAGGATGCTTACAAGAAAGTACGAGCCGGTGCAAGTCTTGTTCAGCTTTATACAGCATTTGCTTACGGGGGACCTGCTCTCATCCCCCAGATAAAGGCTGAATTGTCCGAGTGCTTAGAAAGGGATGGTTACAAGTCCATCAGTGAAGCAGTTGGTGCAGATTGCAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004152 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006220 GO:0006221 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009117 GO:0009165 GO:0009218 GO:0009220 GO:0009259 GO:0009260 GO:0009987 GO:0016491 GO:0016627 GO:0016635 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0034641 GO:0034654 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046390 GO:0046483 GO:0055086 GO:0055114 GO:0071704 GO:0072527 GO:0072528 GO:0090407 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00240 ko01100 map00240 map01100

Pfam Domains

Protein Families

DHO_dh

Protein Analysis

514

Amino Acids

55.0

Weight (kDa)

9.2

Isoelectric Point (pI)

39.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DHO_dh	PF01180	180 - 496	5.5e-90	Dihydroorotate dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0004775)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G23300
fragaria_vesca	FvH4_3g07800 FvH4_3g07800 FvH4_3g07800
malus_domestica	MD05G1301200.v1.1 MD10G1280100.v1.1
prunus_persica	Prupe.4G061300_v2.0.a1 Prupe.6G003700_v2.0.a1 Prupe.6G003700_v2.0.a1 Prupe.6G003700_v2.0.a1
pyrus_communis	pycom05g27900 pycom10g23390
rosa_chinensis	RchiOBHm_Chr5g0010781 RchiOBHm_Chr5g0050031 RchiOBHm_Chr5g0064321
rosa_laevigata	RLG00000031779
rosa_multiflora	Rmu_sc0000109.1_g000020
rosa_roxburghii	Rroxscaffold_1G00065600
rosa_rugosa	Rorug04G0424400 Rorug04G0424500 Rorug04G0452200 Rorug04G0452300 Rorug04G0452400 Rorug06G0421900
rosa_samantha	Rh5AG084600 Rh5BG340700 Rh5CG092100 Rh5DG079500 Rh5DG353400
rosa_wichuraiana	Rw0G000560 Rw0G022270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	235
Acc36I	ACCTGC	2 cut(s)	1042, 1453
Acc65I	GGTACC	1 cut(s)	499
AccB1I	GGYRCC	1 cut(s)	499
AccBSI	CCGCTC	1 cut(s)	99
AccI	GTMKAC	1 cut(s)	1292
AccII	CGCG	1 cut(s)	711
AciI	CCGC	6 cut(s)	97, 113, 116, 124, 182, 357
AclWI	GGATC	1 cut(s)	515
AcsI	RAATTY	1 cut(s)	529
AcuI	CTGAAG	5 cut(s)	624, 685, 816, 1001, 1272
AcvI	CACGTG	1 cut(s)	491
AfaI	GTAC	4 cut(s)	501, 959, 1316, 1392
AfiI	CCNNNNNNNGG	5 cut(s)	677, 1180, 1346, 1347, 1465
AgsI	TTSAA	5 cut(s)	106, 223, 1058, 1148, 1215
AhlI	ACTAGT	1 cut(s)	5
AjnI	CCWGG	3 cut(s)	850, 868, 1000
Alw21I	GWGCWC	3 cut(s)	374, 787, 1066
Alw26I	GTCTC	4 cut(s)	204, 449, 481, 508
AlwI	GGATC	1 cut(s)	515
AlwNI	CAGNNNCTG	2 cut(s)	242, 1226
AoxI	GGCC	2 cut(s)	865, 1094
ApoI	RAATTY	1 cut(s)	529
AseI	ATTAAT	2 cut(s)	33, 44
Asp700I	GAANNNNTTC	1 cut(s)	431
Asp718I	GGTACC	1 cut(s)	499
AspLEI	GCGC	2 cut(s)	236, 711
AspS9I	GGNCC	4 cut(s)	866, 1039, 1094, 1442
AsuHPI	GGTGA	4 cut(s)	848, 981, 985, 1097
AsuII	TTCGAA	1 cut(s)	305
AvaII	GGWCC	2 cut(s)	1039, 1442
AxyI	CCTNAGG	1 cut(s)	337
BanI	GGYRCC	1 cut(s)	499
BauI	CACGAG	1 cut(s)	1065
BbrPI	CACGTG	1 cut(s)	491
BbsI	GAAGAC	1 cut(s)	1146
Bbv12I	GWGCWC	3 cut(s)	374, 787, 1066
BccI	CCATC	4 cut(s)	532, 608, 1490, 1517
BceAI	ACGGC	2 cut(s)	111, 342
BciT130I	CCWGG	3 cut(s)	852, 870, 1002
BclI	TGATCA	1 cut(s)	975
BcoDI	GTCTC	4 cut(s)	204, 449, 481, 508
BcuI	ACTAGT	1 cut(s)	5
BfaI	CTAG	5 cut(s)	6, 173, 504, 539, 878
BfmI	CTRYAG	4 cut(s)	483, 570, 921, 1158
BfuAI	ACCTGC	2 cut(s)	1042, 1453
BglI	GCCNNNNNGGC	1 cut(s)	182
BlpI	GCTNAGC	1 cut(s)	786
Bme1390I	CCNGG	3 cut(s)	852, 870, 1002
Bme18I	GGWCC	2 cut(s)	1039, 1442
BmgT120I	GGNCC	4 cut(s)	866, 1039, 1094, 1442
BmiI	GGNNCC	3 cut(s)	501, 1095, 1443
BmrFI	CCNGG	3 cut(s)	852, 870, 1002
BmrI	ACTGGG	1 cut(s)	949
BmsI	GCATC	4 cut(s)	439, 904, 1366, 1368
BmuI	ACTGGG	1 cut(s)	949
BpiI	GAAGAC	1 cut(s)	1146
BplI	GAGNNNNNCTC	2 cut(s)	638, 670
BpmI	CTGGAG	2 cut(s)	873, 1107
Bpu1102I	GCTNAGC	1 cut(s)	786
Bpu14I	TTCGAA	1 cut(s)	305
BpuEI	CTTGAG	2 cut(s)	9, 1285
Bsa29I	ATCGAT	1 cut(s)	733
BsaAI	YACGTR	2 cut(s)	491, 1010
BsaI	GGTCTC	1 cut(s)	508
BsaJI	CCNNGG	4 cut(s)	177, 868, 1000, 1001
BsaXI	ACNNNNNCTCC	2 cut(s)	351, 381
Bsc4I	CCNNNNNNNGG	5 cut(s)	677, 1180, 1346, 1347, 1465
Bse118I	RCCGGY	1 cut(s)	1397
Bse1I	ACTGG	1 cut(s)	955
Bse21I	CCTNAGG	1 cut(s)	337
Bse3DI	GCAATG	2 cut(s)	1085, 1103
BseBI	CCWGG	3 cut(s)	852, 870, 1002
BseCI	ATCGAT	1 cut(s)	733
BseDI	CCNNGG	4 cut(s)	177, 868, 1000, 1001
BseGI	GGATG	6 cut(s)	385, 454, 814, 1381, 1452, 1501
BseLI	CCNNNNNNNGG	5 cut(s)	677, 1180, 1346, 1347, 1465
BseMI	GCAATG	2 cut(s)	1085, 1103
BseMII	CTCAG	4 cut(s)	197, 444, 492, 800
BseNI	ACTGG	1 cut(s)	955
BseRI	GAGGAG	2 cut(s)	1089, 1103
BsgI	GTGCAG	1 cut(s)	154
Bsh1236I	CGCG	1 cut(s)	711
BshFI	GGCC	2 cut(s)	867, 1096
BshNI	GGYRCC	1 cut(s)	499
BshVI	ATCGAT	1 cut(s)	733
BsiHKAI	GWGCWC	3 cut(s)	374, 787, 1066
BsiSI	CCGG	1 cut(s)	1398
BslFI	GGGAC	2 cut(s)	937, 1455
BslI	CCNNNNNNNGG	5 cut(s)	677, 1180, 1346, 1347, 1465
BsmAI	GTCTC	4 cut(s)	204, 449, 481, 508
BsmFI	GGGAC	2 cut(s)	937, 1455
BsmI	GAATGC	1 cut(s)	604
BsnI	GGCC	2 cut(s)	867, 1096
Bso31I	GGTCTC	1 cut(s)	508
Bsp119I	TTCGAA	1 cut(s)	305
Bsp1286I	GDGCHC	3 cut(s)	374, 787, 1066
Bsp1407I	TGTACA	1 cut(s)	1314
Bsp143I	GATC	2 cut(s)	520, 975
Bsp1720I	GCTNAGC	1 cut(s)	786
Bsp19I	CCATGG	1 cut(s)	177
BspACI	CCGC	6 cut(s)	97, 113, 116, 124, 182, 357
BspANI	GGCC	2 cut(s)	867, 1096
BspCNI	CTCAG	4 cut(s)	198, 445, 491, 799
BspDI	ATCGAT	1 cut(s)	733
BspFNI	CGCG	1 cut(s)	711
BspLI	GGNNCC	3 cut(s)	501, 1095, 1443
BspMAI	CTGCAG	3 cut(s)	487, 925, 1162
BspMI	ACCTGC	2 cut(s)	1042, 1453
BspPI	GGATC	1 cut(s)	515
BspQI	GCTCTTC	1 cut(s)	447
BspT104I	TTCGAA	1 cut(s)	305
BspT107I	GGYRCC	1 cut(s)	499
BspTNI	GGTCTC	1 cut(s)	508
BsrBI	CCGCTC	1 cut(s)	99
BsrDI	GCAATG	2 cut(s)	1085, 1103
BsrFI	RCCGGY	1 cut(s)	1397
BsrGI	TGTACA	1 cut(s)	1314
BsrI	ACTGG	1 cut(s)	955
BssAI	RCCGGY	1 cut(s)	1397
BssECI	CCNNGG	4 cut(s)	177, 868, 1000, 1001
BssMI	GATC	2 cut(s)	520, 975
BssSI	CACGAG	1 cut(s)	1065
BssT1I	CCWWGG	1 cut(s)	177
Bst2BI	CACGAG	1 cut(s)	1065
Bst2UI	CCWGG	3 cut(s)	852, 870, 1002
Bst4CI	ACNGT	3 cut(s)	376, 794, 1210
Bst6I	CTCTTC	2 cut(s)	447, 506
BstAPI	GCANNNNNTGC	2 cut(s)	242, 1526
BstAUI	TGTACA	1 cut(s)	1314
BstBAI	YACGTR	2 cut(s)	491, 1010
BstBI	TTCGAA	1 cut(s)	305
BstC8I	GCNNGC	4 cut(s)	232, 401, 586, 865
BstDEI	CTNAG	7 cut(s)	206, 337, 343, 453, 478, 786, 1486
BstDSI	CCRYGG	1 cut(s)	177
BstF5I	GGATG	6 cut(s)	385, 454, 814, 1381, 1452, 1501
BstFNI	CGCG	1 cut(s)	711
BstHHI	GCGC	2 cut(s)	236, 711
BstKTI	GATC	2 cut(s)	523, 978
BstMAI	GTCTC	4 cut(s)	204, 449, 481, 508
BstMBI	GATC	2 cut(s)	520, 975
BstNI	CCWGG	3 cut(s)	852, 870, 1002
BstNSI	RCATGY	1 cut(s)	234
BstSCI	CCNGG	3 cut(s)	850, 868, 1000
BstSFI	CTRYAG	4 cut(s)	483, 570, 921, 1158
BstSNI	TACGTA	1 cut(s)	1010
BstUI	CGCG	1 cut(s)	711
BstV2I	GAAGAC	1 cut(s)	1146
BstX2I	RGATCY	1 cut(s)	520
BstYI	RGATCY	1 cut(s)	520
Bsu15I	ATCGAT	1 cut(s)	733
Bsu36I	CCTNAGG	1 cut(s)	337
BsuRI	GGCC	2 cut(s)	867, 1096
BsuTUI	ATCGAT	1 cut(s)	733
BtgI	CCRYGG	1 cut(s)	177
BtsCI	GGATG	6 cut(s)	385, 454, 814, 1381, 1452, 1501
BtsI	GCAGTG	1 cut(s)	1372
BtsIMutI	CAGTG	3 cut(s)	372, 1372, 1519
BveI	ACCTGC	2 cut(s)	1042, 1453
Cac8I	GCNNGC	4 cut(s)	232, 401, 586, 865
CaiI	CAGNNNCTG	2 cut(s)	242, 1226
CfoI	GCGC	2 cut(s)	236, 711
Cfr10I	RCCGGY	1 cut(s)	1397
Cfr13I	GGNCC	4 cut(s)	866, 1039, 1094, 1442
ClaI	ATCGAT	1 cut(s)	733
Csp6I	GTAC	4 cut(s)	500, 958, 1315, 1391
CspCI	CAANNNNNGTGG	2 cut(s)	718, 753
CviAII	CATG	2 cut(s)	178, 231
CviQI	GTAC	4 cut(s)	500, 958, 1315, 1391
DdeI	CTNAG	7 cut(s)	206, 337, 343, 453, 478, 786, 1486
DpnI	GATC	2 cut(s)	522, 977
DpnII	GATC	2 cut(s)	520, 975
Eam1104I	CTCTTC	2 cut(s)	447, 506
EarI	CTCTTC	2 cut(s)	447, 506
EciI	GGCGGA	1 cut(s)	372
Eco105I	TACGTA	1 cut(s)	1010
Eco130I	CCWWGG	1 cut(s)	177
Eco31I	GGTCTC	1 cut(s)	508
Eco47I	GGWCC	2 cut(s)	1039, 1442
Eco57I	CTGAAG	5 cut(s)	624, 685, 816, 1001, 1272
Eco72I	CACGTG	1 cut(s)	491
Eco81I	CCTNAGG	1 cut(s)	337
EcoO109I	RGGNCCY	3 cut(s)	1039, 1094, 1442
EcoRII	CCWGG	3 cut(s)	850, 868, 1000
EcoT14I	CCWWGG	1 cut(s)	177
ErhI	CCWWGG	1 cut(s)	177
FaeI	CATG	2 cut(s)	181, 234
FalI	AAGNNNNNCTT	6 cut(s)	811, 843, 1128, 1160, 1325, 1357
FaqI	GGGAC	2 cut(s)	937, 1455
FatI	CATG	2 cut(s)	177, 230
FauI	CCCGC	2 cut(s)	104, 131
FbaI	TGATCA	1 cut(s)	975
FblI	GTMKAC	1 cut(s)	1292
FokI	GGATG	6 cut(s)	392, 461, 821, 1388, 1439, 1508
FspBI	CTAG	5 cut(s)	6, 173, 504, 539, 878
FspI	TGCGCA	1 cut(s)	235
GlaI	GCGC	2 cut(s)	235, 710
GsuI	CTGGAG	2 cut(s)	873, 1107
HaeIII	GGCC	2 cut(s)	867, 1096
HapII	CCGG	1 cut(s)	1398
HhaI	GCGC	2 cut(s)	236, 711
Hin1II	CATG	2 cut(s)	181, 234
Hin6I	GCGC	2 cut(s)	234, 709
HinP1I	GCGC	2 cut(s)	234, 709
HincII	GTYRAC	1 cut(s)	324
HindII	GTYRAC	1 cut(s)	324
HinfI	GANTC	5 cut(s)	292, 427, 886, 1223, 1336
HpaII	CCGG	1 cut(s)	1398
HphI	GGTGA	4 cut(s)	848, 981, 985, 1097
Hpy166II	GTNNAC	3 cut(s)	324, 1235, 1293
Hpy188I	TCNGA	3 cut(s)	704, 1252, 1480
Hpy188III	TCNNGA	5 cut(s)	26, 446, 1067, 1124, 1215
Hpy8I	GTNNAC	3 cut(s)	324, 1235, 1293
HpyAV	CCTTC	5 cut(s)	51, 112, 158, 546, 1184
HpyCH4III	ACNGT	3 cut(s)	376, 794, 1210
HpyCH4IV	ACGT	2 cut(s)	490, 1009
HpyF3I	CTNAG	7 cut(s)	206, 337, 343, 453, 478, 786, 1486
HpySE526I	ACGT	2 cut(s)	490, 1009
Hsp92II	CATG	2 cut(s)	181, 234
HspAI	GCGC	2 cut(s)	234, 709
KpnI	GGTACC	1 cut(s)	503
Ksp22I	TGATCA	1 cut(s)	975
Kzo9I	GATC	2 cut(s)	520, 975
LguI	GCTCTTC	1 cut(s)	447
LmnI	GCTCC	5 cut(s)	104, 244, 289, 359, 1126
LweI	GCATC	4 cut(s)	439, 904, 1366, 1368
MaeI	CTAG	5 cut(s)	6, 173, 504, 539, 878
MaeII	ACGT	2 cut(s)	490, 1009
MaeIII	GTNAC	3 cut(s)	658, 1005, 1499
MalI	GATC	2 cut(s)	522, 977
MbiI	CCGCTC	1 cut(s)	99
MboI	GATC	2 cut(s)	520, 975
MfeI	CAATTG	1 cut(s)	89
MflI	RGATCY	1 cut(s)	520
MhlI	GDGCHC	3 cut(s)	374, 787, 1066
MmeI	TCCRAC	2 cut(s)	588, 786
MroXI	GAANNNNTTC	1 cut(s)	431
MseI	TTAA	6 cut(s)	15, 33, 44, 744, 1034, 1343
MspA1I	CMGCKG	1 cut(s)	482
MspI	CCGG	1 cut(s)	1398
MspR9I	CCNGG	3 cut(s)	852, 870, 1002
MunI	CAATTG	1 cut(s)	89
Mva1269I	GAATGC	1 cut(s)	604
MvaI	CCWGG	3 cut(s)	852, 870, 1002
MvnI	CGCG	1 cut(s)	711
NcoI	CCATGG	1 cut(s)	177
NdeII	GATC	2 cut(s)	520, 975
NlaIII	CATG	2 cut(s)	181, 234
NlaIV	GGNNCC	3 cut(s)	501, 1095, 1443
NsbI	TGCGCA	1 cut(s)	235
NspI	RCATGY	1 cut(s)	234
NspV	TTCGAA	1 cut(s)	305
PaeI	GCATGC	1 cut(s)	234
PasI	CCCWGGG	1 cut(s)	1001
PciSI	GCTCTTC	1 cut(s)	447
PctI	GAATGC	1 cut(s)	604
PdmI	GAANNNNTTC	1 cut(s)	431
PfeI	GAWTC	5 cut(s)	292, 427, 886, 1223, 1336
PmaCI	CACGTG	1 cut(s)	491
PmlI	CACGTG	1 cut(s)	491
Ppu21I	YACGTR	2 cut(s)	491, 1010
PpuMI	RGGWCCY	2 cut(s)	1039, 1442
PshBI	ATTAAT	2 cut(s)	33, 44
Psp5II	RGGWCCY	2 cut(s)	1039, 1442
Psp6I	CCWGG	3 cut(s)	850, 868, 1000
PspCI	CACGTG	1 cut(s)	491
PspGI	CCWGG	3 cut(s)	850, 868, 1000
PspN4I	GGNNCC	3 cut(s)	501, 1095, 1443
PspPI	GGNCC	4 cut(s)	866, 1039, 1094, 1442
PspPPI	RGGWCCY	2 cut(s)	1039, 1442
PsrI	GAACNNNNNNTAC	2 cut(s)	651, 683
PstI	CTGCAG	3 cut(s)	487, 925, 1162
PstNI	CAGNNNCTG	2 cut(s)	242, 1226
PsuI	RGATCY	1 cut(s)	520
PvuII	CAGCTG	1 cut(s)	482
RsaI	GTAC	4 cut(s)	501, 959, 1316, 1392
RsaNI	GTAC	4 cut(s)	500, 958, 1315, 1391
SapI	GCTCTTC	1 cut(s)	447
SaqAI	TTAA	6 cut(s)	15, 33, 44, 744, 1034, 1343
Sau3AI	GATC	2 cut(s)	520, 975
Sau96I	GGNCC	4 cut(s)	866, 1039, 1094, 1442
ScrFI	CCNGG	3 cut(s)	852, 870, 1002
SduI	GDGCHC	3 cut(s)	374, 787, 1066
SfaNI	GCATC	4 cut(s)	439, 904, 1366, 1368
SfcI	CTRYAG	4 cut(s)	483, 570, 921, 1158
SfuI	TTCGAA	1 cut(s)	305
SinI	GGWCC	2 cut(s)	1039, 1442
SmlI	CTYRAG	2 cut(s)	24, 1264
SmoI	CTYRAG	2 cut(s)	24, 1264
SnaBI	TACGTA	1 cut(s)	1010
SpeI	ACTAGT	1 cut(s)	5
SphI	GCATGC	1 cut(s)	234
SsiI	CCGC	6 cut(s)	97, 113, 116, 124, 182, 357
SspMI	CTAG	5 cut(s)	6, 173, 504, 539, 878
StyD4I	CCNGG	3 cut(s)	850, 868, 1000
StyI	CCWWGG	1 cut(s)	177
TaaI	ACNGT	3 cut(s)	376, 794, 1210
TaiI	ACGT	2 cut(s)	493, 1012
TaqI	TCGA	4 cut(s)	39, 305, 733, 1284
TatI	WGTACW	1 cut(s)	1314
TauI	GCSGC	3 cut(s)	115, 118, 185
TfiI	GAWTC	5 cut(s)	292, 427, 886, 1223, 1336
Tru1I	TTAA	6 cut(s)	15, 33, 44, 744, 1034, 1343
Tru9I	TTAA	6 cut(s)	15, 33, 44, 744, 1034, 1343
TscAI	CASTG	3 cut(s)	379, 1372, 1519
TspDTI	ATGAA	5 cut(s)	396, 825, 932, 975, 1086
TspGWI	ACGGA	2 cut(s)	120, 1200
TspRI	CASTG	3 cut(s)	379, 1372, 1519
VpaK11BI	GGWCC	2 cut(s)	1039, 1442
VspI	ATTAAT	2 cut(s)	33, 44
XapI	RAATTY	1 cut(s)	529
XceI	RCATGY	1 cut(s)	234
XmiI	GTMKAC	1 cut(s)	1292
XmnI	GAANNNNTTC	1 cut(s)	431
XspI	CTAG	5 cut(s)	6, 173, 504, 539, 878

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.