Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000004

Orthogroup ID

OG0004775

Physical Location & Seq

Genomic Coordinates Forward (+)

58359844 .. 58364011

Gene Structure 4168 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug04G0424500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3048 bp

ATGAGTGACTCAGAAGAAGATGACAGGAGTTCAGAGAGTTCAGATAACTGCACTGATACAACGTTTAACCAAACCCTGCAAAAAAAATTTGACGACATCAGAAAGAGGAAGCGACAACAGGCAGAACATCATTCTGATCAAGAATCTGCTGAAGAAGAAGTGAATGAAGATTCAGGACGACAATCAGAAGAAGAATCCGAATCAGAAGGTGAACAGACAACGAAGAGGTTTGTAATCAAAACAAGACAACAACCAAAAAGGAAACCTGTTCAAGAGGAAGAGGATTCAGAAGAAGAAAAAACAAAGAGGAAGAAGGCCAAGAAGAAAAAGAAAGCTGAAATCAAGAAGGAAGAGCAGAAAAGTGAGACCGAAAAAGCAAAGATTGAATGGAAGCAACAGAAGTGCACGCTCAGTGCATTCTGGAGAATGGTCAATGCACACAAGGATAGAATACCAGACAAGACAAAGGAGATTTTGAAGGGTACAGACTTTGGAGAAATGATGGAACCGTTCTGGCAGGACAAGATAACCGAGATCCAGCTACACAAGCATGAAGCGGACCTGGAGATACTCATGAGGCACTTCGACCGCACAGACAACAAGTGGAAGTTTGGGGATGTTGTTATGGAAATAACGGAGGAGGATATCACGACTTTATTTCACCTCCCGGCCGAAGGAGAAGTGTTCAATGTGAACAGGAGGGTGGTGAGGGAAGAGATGGAAGACTCTCCAATTTTTGGCAGCCCTTTAAAGACGCATGCTGTCCTCAGAACAAAGGTGGAATCGTATTTGGTAACTGAGTTGACAAAGCCGGCAAAAGAGAAAGATGCCAGGAAGATAGCCGTGCTAATGATCACATATCTATTCAGCACATTCTTTTTCACCCGAACCGGTGCTCAGATCACATGGGATATGGTCGCCGTATGTGAACGGATTGAGAAGATAAACATGTACAACTGGCCAAGATTAATTCTGAACTTCTTGATGGAAGGGCTACAAAAGTATAGGAGGAACAGTCCATCTGTTTTGAATGGATGCCTTCTTCTCATATATTACTGGTTCCTTGAGAAGACCAGGGCAAAGACCTGGATACTTGGAAAGCAGAATGAAACTCCACGATTCATCCGATGGTCGATAAAGGAAATATTTAGCCTGGAACAGCTGTACAGGAACGAGAACTTAGCCGTGGATCTGATAAAAGCTGGACCGTGGCAAGGGAAAATTGGACTGGAAGACCTCGAGATGGGTGATGAGGTGCAGGACACACCAAGCTTTGACAACTGGGTGCCCCAGGCAAGCCAAACGGAAGAGGAACAAAATGAAAGGCTGACGGGGAATATCAAGGAACTAATCAGGGAGATGGAAGCAGCAATTGGTGAGTCAAAGCCCACCAAAGAAATGGAGGCAAAGCTAGGAAGGCTTGCCAAAGCAAACGAGGAACTGAATGCACAGAACAAAGATCTATGGGTCAAACTAAAGGTTGCCGATGAGAGGATTAAAGAGCTGGAAATTGAAAAGAGGGCAAAAAATAACCTCATCAGGGCGTTGTACATCCGGCTTGAAAAGGAGAAAGGAGAGGTCCCCCCACCACAAAAACAACTTGAGATAGTTCCTTTCATGCCGAAAGTGGTCCCCCAAGATGAAGTTGAGGATGAAAATCCAAACTTTGGACAAAGCGTAGGAGAGGAGACCCATTATGCAGCCAGTGTGGGAAAGTCGACGGACACGGTGACGTCAGCGTTTCCTATTGCAGAGGAAATACCTGGACAACAAGATGAAAGCCGTTTACAACCGGGGCAACAAGTACATGGGAATGAAGAGGAAGATGAACAGCTCGATAACATCATAAGGATTATTGCTGAGAAGGAAGCGATGGGAGTAATGGGAAAGGAAAAACCGAGGGAAGGGACCCCAGCCAAACAGGAACCACAGAAGAAAACTCCGGAGATACATTCTATAGTGAAGAACGTGAAGACATACCGGAGGGCTGCCAAGAAGGCTAGAGAGGAGGAGTGGGAGTACGACACTCCGGAAGAAGCAAAAATTACGAAGAGAGCTGCACCTAAGAAAGCAGGAATCATGCAGCAAGCCCAGAGAAAAATGTTGAACAATATCAAGATCAAGGGTGTTAAATGCGATAAGCCAACATGGAGGACACTGGACCCGGCAGTGGCAAGGCACTATAGAGACTTCTTCAACGTTGCTGTGAAAGACACGACCGAGTACTGGACCAGCATTGATCTTCTACGCCGGATCACCAAGCACGACCTAAGAGTAATCATCCAAGAGGGGGACATCGAAACTGATGTGATCAGCGTTTATATGGAGTTGTTGAAGTCTGATGCAGAAAAGCTAAATGCGCAAGTGGGATTCCTCACAGTCGACGCAGGGTACTATGCTATAAAGTATGAACAGACCAAAGAACAAGATGAGGACACTAGACCTTTTGAGTGTGGTGTCGAGACAATGATTTATGAACCCCTGTGGTCAGTCTTCCGTTTCAAAAAGGTGCTAGTACCAATTCACCACACAACAAGCATGCACTACACATTGCTGGTAATTGACAATGAAACAAGGAGGTTCACACATATGAATTCATTGAGGCCACCAATCAATGAATTCACCAATGAGGAGAAGTACCAACTGAATGCAGCAAGAGTGGTAAAGCACATACAGAAATTCATACATGTTGTGAACTTGACTAAGACGAGAATACGGGGCGAAAGCCAAGATCCAAATATCACTCGAGAAAAATGCAAAGGCGAAGATGATAAGGAAAACCTGATTATTGTTGAAGAAGCAATGACCGAGGAGGAAAAACAAACCAGAAACTGGATCTTACAGAATAACGTTGTGGAGACAGACTATGAACTCTTTGAAGACTTTGACTGCCCGCAACAGAACACATCATCTGGTGATTGCGGGCCCTTTATGCTCCATTACATGGAGAGCATAGTAAATGGCATAGAACCAACCAAGGAAGGCGGGGACAACATGAGGAAGAGATTGCTTGAGAGGTTCATGCACCTACTATTGGGCAGAAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004152 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006220 GO:0006221 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009117 GO:0009165 GO:0009218 GO:0009220 GO:0009259 GO:0009260 GO:0009987 GO:0016491 GO:0016627 GO:0016635 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0034641 GO:0034654 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046390 GO:0046483 GO:0055086 GO:0055114 GO:0071704 GO:0072527 GO:0072528 GO:0090407 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00240 ko01100 map00240 map01100

Pfam Domains

Protein Families

DHO_dh

Protein Analysis

1015

Amino Acids

118.57

Weight (kDa)

5.6

Isoelectric Point (pI)

55.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMD	PF10536	209 - 381	5.9e-06	Plant mobile domain
Peptidase_C48	PF02902	766 - 993	4.1e-07	Ulp1 protease family, C-terminal catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004775)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G23300
fragaria_vesca	FvH4_3g07800 FvH4_3g07800 FvH4_3g07800
malus_domestica	MD05G1301200.v1.1 MD10G1280100.v1.1
prunus_persica	Prupe.4G061300_v2.0.a1 Prupe.6G003700_v2.0.a1 Prupe.6G003700_v2.0.a1 Prupe.6G003700_v2.0.a1
pyrus_communis	pycom05g27900 pycom10g23390
rosa_chinensis	RchiOBHm_Chr5g0010781 RchiOBHm_Chr5g0050031 RchiOBHm_Chr5g0064321
rosa_laevigata	RLG00000031779
rosa_multiflora	Rmu_sc0000109.1_g000020
rosa_roxburghii	Rroxscaffold_1G00065600
rosa_rugosa	Rorug04G0424400 Rorug04G0424500 Rorug04G0452200 Rorug04G0452300 Rorug04G0452400 Rorug06G0421900
rosa_samantha	Rh5AG084600 Rh5BG340700 Rh5CG092100 Rh5DG079500 Rh5DG353400
rosa_wichuraiana	Rw0G000560 Rw0G022270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1736
Acc16I	TGCGCA	1 cut(s)	2361
AccB1I	GGYRCC	1 cut(s)	1285
AccB7I	CCANNNNNTGG	4 cut(s)	737, 1667, 2832, 2944
AccI	GTMKAC	2 cut(s)	1718, 2382
AccIII	TCCGGA	2 cut(s)	1942, 2029
AciI	CCGC	5 cut(s)	557, 589, 2894, 2922, 2985
AclI	AACGTT	3 cut(s)	62, 2199, 2850
AclWI	GGATC	5 cut(s)	529, 1197, 2261, 2726, 2843
AcoI	YGGCCR	2 cut(s)	669, 959
AcsI	RAATTY	4 cut(s)	86, 2593, 2618, 2678
AcuI	CTGAAG	1 cut(s)	171
AcyI	GRCGYC	1 cut(s)	1733
AflIII	ACRYGT	2 cut(s)	948, 2686
AgeI	ACCGGT	1 cut(s)	890
AjnI	CCWGG	7 cut(s)	561, 830, 1073, 1085, 1152, 1290, 1762
AjuI	GAANNNNNNNTTGG	2 cut(s)	1356, 1388
Alw21I	GWGCWC	2 cut(s)	407, 898
Alw26I	GTCTC	5 cut(s)	359, 1682, 2181, 2456, 2852
Alw44I	GTGCAC	1 cut(s)	403
AlwI	GGATC	5 cut(s)	529, 1197, 2261, 2726, 2843
AlwNI	CAGNNNCTG	1 cut(s)	2832
Ama87I	CYCGRG	2 cut(s)	1238, 2745
Aor13HI	TCCGGA	2 cut(s)	1942, 2029
AoxI	GGCC	5 cut(s)	315, 669, 959, 2603, 2924
ApaI	GGGCCC	1 cut(s)	2928
ApaLI	GTGCAC	1 cut(s)	403
ApeKI	GCWGC	7 cut(s)	741, 1367, 1700, 1988, 2057, 2083, 2651
ApoI	RAATTY	4 cut(s)	86, 2593, 2618, 2678
AseI	ATTAAT	1 cut(s)	968
AsiGI	ACCGGT	1 cut(s)	890
AspLEI	GCGC	1 cut(s)	2362
AspS9I	GGNCC	9 cut(s)	559, 1205, 1579, 1630, 1908, 2161, 2229, 2924, 2925
AsuC2I	CCSGG	3 cut(s)	668, 1794, 2165
AvaI	CYCGRG	2 cut(s)	1238, 2745
AvaII	GGWCC	7 cut(s)	559, 1205, 1579, 1630, 1908, 2161, 2229
BaeGI	GKGCMC	3 cut(s)	407, 1290, 2928
BalI	TGGCCA	1 cut(s)	961
BanI	GGYRCC	1 cut(s)	1285
BanII	GRGCYC	1 cut(s)	2928
BbsI	GAAGAC	6 cut(s)	729, 1076, 1239, 1979, 2485, 2886
Bbv12I	GWGCWC	2 cut(s)	407, 898
BbvI	GCAGC	7 cut(s)	753, 1379, 1712, 1975, 2044, 2095, 2663
BccI	CCATC	8 cut(s)	496, 712, 979, 1027, 1122, 1237, 1354, 1867
BceAI	ACGGC	4 cut(s)	827, 905, 1169, 1767
BcgI	CGANNNNNNTGC	2 cut(s)	2736, 2770
BciT130I	CCWGG	7 cut(s)	563, 832, 1075, 1087, 1154, 1292, 1764
BciVI	GTATCC	1 cut(s)	1083
BclI	TGATCA	3 cut(s)	136, 852, 2310
BcnI	CCSGG	3 cut(s)	668, 1794, 2165
BcoDI	GTCTC	5 cut(s)	359, 1682, 2181, 2456, 2852
BfaI	CTAG	4 cut(s)	1412, 2001, 2439, 2513
BfmI	CTRYAG	2 cut(s)	1956, 2182
BfuI	GTATCC	1 cut(s)	1083
BglI	GCCNNNNNGGC	1 cut(s)	1997
BglII	AGATCT	1 cut(s)	1459
BisI	GCNGC	7 cut(s)	742, 1368, 1701, 1989, 2058, 2084, 2652
BlsI	GCNGC	7 cut(s)	743, 1369, 1702, 1990, 2059, 2085, 2653
BmcAI	AGTACT	1 cut(s)	2225
Bme18I	GGWCC	7 cut(s)	559, 1205, 1579, 1630, 1908, 2161, 2229
BmeT110I	CYCGRG	2 cut(s)	1238, 2745
BmgT120I	GGNCC	9 cut(s)	559, 1205, 1579, 1630, 1908, 2161, 2229, 2924, 2925
BmrI	ACTGGG	1 cut(s)	1291
BmsI	GCATC	3 cut(s)	817, 1025, 2332
BmuI	ACTGGG	1 cut(s)	1291
BpiI	GAAGAC	6 cut(s)	729, 1076, 1239, 1979, 2485, 2886
BpmI	CTGGAG	2 cut(s)	442, 584
BpuEI	CTTGAG	3 cut(s)	1085, 1622, 3032
BpuMI	CCSGG	3 cut(s)	668, 1794, 2165
BsaBI	GATNNNNATC	1 cut(s)	1656
BsaHI	GRCGYC	1 cut(s)	1733
BsaI	GGTCTC	2 cut(s)	359, 1682
BsaJI	CCNNGG	8 cut(s)	1074, 1185, 1208, 1290, 1793, 1898, 2808, 2976
BsaWI	WCCGGW	4 cut(s)	890, 1942, 1980, 2029
BsaXI	ACNNNNNCTCC	4 cut(s)	1000, 1030, 2003, 2033
Bse118I	RCCGGY	2 cut(s)	811, 890
Bse1I	ACTGG	8 cut(s)	962, 1061, 1233, 1286, 1704, 2163, 2231, 2837
Bse3DI	GCAATG	2 cut(s)	2549, 2808
Bse8I	GATNNNNATC	1 cut(s)	1656
BseAI	TCCGGA	2 cut(s)	1942, 2029
BseBI	CCWGG	7 cut(s)	563, 832, 1075, 1087, 1154, 1292, 1764
BseDI	CCNNGG	8 cut(s)	1074, 1185, 1208, 1290, 1793, 1898, 2808, 2976
BseGI	GGATG	6 cut(s)	622, 1040, 1122, 1551, 1657, 2280
BseJI	GATNNNNATC	1 cut(s)	1656
BseMI	GCAATG	2 cut(s)	2549, 2808
BseMII	CTCAG	6 cut(s)	24, 424, 781, 789, 911, 1851
BseNI	ACTGG	8 cut(s)	962, 1061, 1233, 1286, 1704, 2163, 2231, 2837
BseRI	GAGGAG	6 cut(s)	653, 1700, 2021, 2024, 2645, 2825
BseSI	GKGCMC	3 cut(s)	407, 1290, 2928
BseX3I	CGGCCG	1 cut(s)	669
BseXI	GCAGC	7 cut(s)	753, 1379, 1712, 1975, 2044, 2095, 2663
BseYI	CCCAGC	1 cut(s)	1912
BsgI	GTGCAG	3 cut(s)	34, 1277, 2043
Bsh1285I	CGRYCG	3 cut(s)	589, 672, 2220
BshFI	GGCC	5 cut(s)	317, 671, 961, 2605, 2926
BshNI	GGYRCC	1 cut(s)	1285
BshTI	ACCGGT	1 cut(s)	890
BsiEI	CGRYCG	3 cut(s)	589, 672, 2220
BsiHKAI	GWGCWC	2 cut(s)	407, 898
BsiHKCI	CYCGRG	2 cut(s)	1238, 2745
BslFI	GGGAC	5 cut(s)	1565, 1616, 1921, 2306, 3002
BsmAI	GTCTC	5 cut(s)	359, 1682, 2181, 2456, 2852
BsmFI	GGGAC	5 cut(s)	1565, 1616, 1921, 2306, 3002
BsmI	GAATGC	3 cut(s)	416, 1450, 2653
BsnI	GGCC	5 cut(s)	317, 671, 961, 2605, 2926
Bso31I	GGTCTC	2 cut(s)	359, 1682
BsoBI	CYCGRG	2 cut(s)	1238, 2745
Bsp120I	GGGCCC	1 cut(s)	2924
Bsp1286I	GDGCHC	4 cut(s)	407, 898, 1290, 2928
Bsp13I	TCCGGA	2 cut(s)	1942, 2029
Bsp1407I	TGTACA	3 cut(s)	951, 1164, 1548
BspACI	CCGC	5 cut(s)	557, 589, 2894, 2922, 2985
BspANI	GGCC	5 cut(s)	317, 671, 961, 2605, 2926
BspCNI	CTCAG	6 cut(s)	23, 423, 780, 790, 910, 1852
BspEI	TCCGGA	2 cut(s)	1942, 2029
BspHI	TCATGA	1 cut(s)	573
BspPI	GGATC	5 cut(s)	529, 1197, 2261, 2726, 2843
BspQI	GCTCTTC	1 cut(s)	345
BspT107I	GGYRCC	1 cut(s)	1285
BspTNI	GGTCTC	2 cut(s)	359, 1682
BsrDI	GCAATG	2 cut(s)	2549, 2808
BsrFI	RCCGGY	2 cut(s)	811, 890
BsrGI	TGTACA	3 cut(s)	951, 1164, 1548
BsrI	ACTGG	8 cut(s)	962, 1061, 1233, 1286, 1704, 2163, 2231, 2837
BssAI	RCCGGY	2 cut(s)	811, 890
BssECI	CCNNGG	8 cut(s)	1074, 1185, 1208, 1290, 1793, 1898, 2808, 2976
BssNI	GRCGYC	1 cut(s)	1733
BssT1I	CCWWGG	1 cut(s)	2976
Bst2UI	CCWGG	7 cut(s)	563, 832, 1075, 1087, 1154, 1292, 1764
Bst4CI	ACNGT	5 cut(s)	510, 1016, 1209, 1729, 2380
Bst6I	CTCTTC	8 cut(s)	218, 273, 345, 708, 1302, 1812, 2045, 2996
BstACI	GRCGYC	1 cut(s)	1733
BstAUI	TGTACA	3 cut(s)	951, 1164, 1548
BstC8I	GCNNGC	9 cut(s)	407, 759, 813, 1297, 1422, 2088, 2540, 2894, 2924
BstDSI	CCRYGG	2 cut(s)	1185, 1208
BstF5I	GGATG	6 cut(s)	622, 1040, 1122, 1551, 1657, 2280
BstHHI	GCGC	1 cut(s)	2362
BstMAI	GTCTC	5 cut(s)	359, 1682, 2181, 2456, 2852
BstMCI	CGRYCG	3 cut(s)	589, 672, 2220
BstMWI	GCNNNNNNNGC	5 cut(s)	547, 1417, 1997, 2359, 2932
BstNI	CCWGG	7 cut(s)	563, 832, 1075, 1087, 1154, 1292, 1764
BstNSI	RCATGY	4 cut(s)	761, 952, 2542, 2690
BstSFI	CTRYAG	2 cut(s)	1956, 2182
BstSLI	GKGCMC	3 cut(s)	407, 1290, 2928
BstV1I	GCAGC	7 cut(s)	753, 1379, 1712, 1975, 2044, 2095, 2663
BstV2I	GAAGAC	6 cut(s)	729, 1076, 1239, 1979, 2485, 2886
BstX2I	RGATCY	5 cut(s)	534, 1189, 1459, 2731, 2835
BstXI	CCANNNNNNTGG	1 cut(s)	1399
BstYI	RGATCY	5 cut(s)	534, 1189, 1459, 2731, 2835
BstZI	CGGCCG	1 cut(s)	669
BsuI	GTATCC	1 cut(s)	1083
BsuRI	GGCC	5 cut(s)	317, 671, 961, 2605, 2926
BtgI	CCRYGG	2 cut(s)	1185, 1208
BtgZI	GCGATG	1 cut(s)	1886
BtsCI	GGATG	6 cut(s)	622, 1040, 1122, 1551, 1657, 2280
BtsI	GCAGTG	1 cut(s)	2175
BtsIMutI	CAGTG	5 cut(s)	51, 418, 1711, 2156, 2175
Cac8I	GCNNGC	9 cut(s)	407, 759, 813, 1297, 1422, 2088, 2540, 2894, 2924
CaiI	CAGNNNCTG	1 cut(s)	2832
CciI	TCATGA	1 cut(s)	573
CfoI	GCGC	1 cut(s)	2362
Cfr10I	RCCGGY	2 cut(s)	811, 890
Cfr13I	GGNCC	9 cut(s)	559, 1205, 1579, 1630, 1908, 2161, 2229, 2924, 2925
CseI	GACGC	2 cut(s)	763, 2393
CspAI	ACCGGT	1 cut(s)	890
DraI	TTTAAA	1 cut(s)	750
EaeI	YGGCCR	2 cut(s)	669, 959
EagI	CGGCCG	1 cut(s)	669
Eam1104I	CTCTTC	8 cut(s)	218, 273, 345, 708, 1302, 1812, 2045, 2996
EarI	CTCTTC	8 cut(s)	218, 273, 345, 708, 1302, 1812, 2045, 2996
EclXI	CGGCCG	1 cut(s)	669
Eco130I	CCWWGG	1 cut(s)	2976
Eco24I	GRGCYC	1 cut(s)	2928
Eco31I	GGTCTC	2 cut(s)	359, 1682
Eco32I	GATATC	1 cut(s)	646
Eco47I	GGWCC	7 cut(s)	559, 1205, 1579, 1630, 1908, 2161, 2229
Eco52I	CGGCCG	1 cut(s)	669
Eco57I	CTGAAG	1 cut(s)	171
Eco88I	CYCGRG	2 cut(s)	1238, 2745
EcoO109I	RGGNCCY	3 cut(s)	1579, 1908, 2925
EcoRI	GAATTC	2 cut(s)	2593, 2618
EcoRII	CCWGG	7 cut(s)	561, 830, 1073, 1085, 1152, 1290, 1762
EcoRV	GATATC	1 cut(s)	646
EcoT14I	CCWWGG	1 cut(s)	2976
EcoT38I	GRGCYC	1 cut(s)	2928
ErhI	CCWWGG	1 cut(s)	2976
FaqI	GGGAC	5 cut(s)	1565, 1616, 1921, 2306, 3002
FauI	CCCGC	3 cut(s)	2901, 2915, 2978
FauNDI	CATATG	1 cut(s)	2589
FbaI	TGATCA	3 cut(s)	136, 852, 2310
FblI	GTMKAC	2 cut(s)	1718, 2382
Fnu4HI	GCNGC	7 cut(s)	742, 1368, 1701, 1989, 2058, 2084, 2652
FokI	GGATG	6 cut(s)	629, 1047, 1109, 1538, 1664, 2267
FriOI	GRGCYC	1 cut(s)	2928
Fsp4HI	GCNGC	7 cut(s)	742, 1368, 1701, 1989, 2058, 2084, 2652
FspBI	CTAG	4 cut(s)	1412, 2001, 2439, 2513
FspI	TGCGCA	1 cut(s)	2361
GlaI	GCGC	1 cut(s)	2361
GluI	GCNGC	7 cut(s)	742, 1368, 1701, 1989, 2058, 2084, 2652
GsaI	CCCAGC	1 cut(s)	1916
GsuI	CTGGAG	2 cut(s)	442, 584
HaeIII	GGCC	5 cut(s)	317, 671, 961, 2605, 2926
HgaI	GACGC	2 cut(s)	763, 2393
HhaI	GCGC	1 cut(s)	2362
Hin1I	GRCGYC	1 cut(s)	1733
Hin6I	GCGC	1 cut(s)	2360
HinP1I	GCGC	1 cut(s)	2360
HincII	GTYRAC	3 cut(s)	804, 1719, 2383
HindII	GTYRAC	3 cut(s)	804, 1719, 2383
HindIII	AAGCTT	1 cut(s)	1270
Hpy99I	CGWCG	2 cut(s)	1723, 2387
HpyCH4III	ACNGT	5 cut(s)	510, 1016, 1209, 1729, 2380
HpyCH4IV	ACGT	5 cut(s)	62, 1733, 1968, 2199, 2850
HpyF10VI	GCNNNNNNNGC	5 cut(s)	547, 1417, 1997, 2359, 2932
HpySE526I	ACGT	5 cut(s)	62, 1733, 1968, 2199, 2850
Hsp92I	GRCGYC	1 cut(s)	1733
HspAI	GCGC	1 cut(s)	2360
KflI	GGGWCCC	1 cut(s)	1908
Kpn2I	TCCGGA	2 cut(s)	1942, 2029
KroI	GCCGGC	1 cut(s)	811
KroNI	GCCGGC	1 cut(s)	813
Ksp22I	TGATCA	3 cut(s)	136, 852, 2310
LguI	GCTCTTC	1 cut(s)	345
LmnI	GCTCC	1 cut(s)	2940
Lsp1109I	GCAGC	7 cut(s)	753, 1379, 1712, 1975, 2044, 2095, 2663
LweI	GCATC	3 cut(s)	817, 1025, 2332
MaeI	CTAG	4 cut(s)	1412, 2001, 2439, 2513
MaeII	ACGT	5 cut(s)	62, 1733, 1968, 2199, 2850
MaeIII	GTNAC	3 cut(s)	5, 793, 1729
MfeI	CAATTG	1 cut(s)	1371
MflI	RGATCY	5 cut(s)	534, 1189, 1459, 2731, 2835
MhlI	GDGCHC	4 cut(s)	407, 898, 1290, 2928
MlsI	TGGCCA	1 cut(s)	961
MluNI	TGGCCA	1 cut(s)	961
MlyI	GAGTC	3 cut(s)	2, 719, 1388
Mox20I	TGGCCA	1 cut(s)	961
MroI	TCCGGA	2 cut(s)	1942, 2029
MroNI	GCCGGC	1 cut(s)	811
MscI	TGGCCA	1 cut(s)	961
MseI	TTAA	5 cut(s)	66, 749, 968, 1497, 2130
MslI	CAYNNNNRTG	3 cut(s)	549, 1812, 2588
Msp20I	TGGCCA	1 cut(s)	961
MspA1I	CMGCKG	1 cut(s)	1162
MunI	CAATTG	1 cut(s)	1371
Mva1269I	GAATGC	3 cut(s)	416, 1450, 2653
MvaI	CCWGG	7 cut(s)	563, 832, 1075, 1087, 1154, 1292, 1764
MwoI	GCNNNNNNNGC	5 cut(s)	547, 1417, 1997, 2359, 2932
NaeI	GCCGGC	1 cut(s)	813
NciI	CCSGG	3 cut(s)	668, 1794, 2165
NdeI	CATATG	1 cut(s)	2589
NgoMIV	GCCGGC	1 cut(s)	811
NmuCI	GTSAC	2 cut(s)	5, 1729
NsbI	TGCGCA	1 cut(s)	2361
NspI	RCATGY	4 cut(s)	761, 952, 2542, 2690
PaeI	GCATGC	2 cut(s)	761, 2542
PaeR7I	CTCGAG	2 cut(s)	1238, 2745
PagI	TCATGA	1 cut(s)	573
PciI	ACATGT	2 cut(s)	948, 2686
PciSI	GCTCTTC	1 cut(s)	345
PcsI	WCGNNNNNNNCGW	1 cut(s)	1123
PctI	GAATGC	3 cut(s)	416, 1450, 2653
PdiI	GCCGGC	1 cut(s)	813
PfeI	GAWTC	9 cut(s)	143, 170, 194, 200, 284, 782, 1119, 2076, 2370
PflMI	CCANNNNNTGG	4 cut(s)	737, 1667, 2832, 2944
PinAI	ACCGGT	1 cut(s)	890
PkrI	GCNGC	7 cut(s)	743, 1369, 1702, 1990, 2059, 2085, 2653
PleI	GAGTC	3 cut(s)	2, 719, 1387
PpsI	GAGTC	3 cut(s)	2, 719, 1387
PpuMI	RGGWCCY	2 cut(s)	1579, 1908
PscI	ACATGT	2 cut(s)	948, 2686
PshBI	ATTAAT	1 cut(s)	968
Psp1406I	AACGTT	3 cut(s)	62, 2199, 2850
Psp5II	RGGWCCY	2 cut(s)	1579, 1908
Psp6I	CCWGG	7 cut(s)	561, 830, 1073, 1085, 1152, 1290, 1762
PspFI	CCCAGC	1 cut(s)	1912
PspGI	CCWGG	7 cut(s)	561, 830, 1073, 1085, 1152, 1290, 1762
PspOMI	GGGCCC	1 cut(s)	2924
PspPI	GGNCC	9 cut(s)	559, 1205, 1579, 1630, 1908, 2161, 2229, 2924, 2925
PspPPI	RGGWCCY	2 cut(s)	1579, 1908
PstNI	CAGNNNCTG	1 cut(s)	2832
PsuI	RGATCY	5 cut(s)	534, 1189, 1459, 2731, 2835
PvuII	CAGCTG	1 cut(s)	1162
RseI	CAYNNNNRTG	3 cut(s)	549, 1812, 2588
SalI	GTCGAC	2 cut(s)	1717, 2381
SapI	GCTCTTC	1 cut(s)	345
SaqAI	TTAA	5 cut(s)	66, 749, 968, 1497, 2130
SatI	GCNGC	7 cut(s)	742, 1368, 1701, 1989, 2058, 2084, 2652
Sau96I	GGNCC	9 cut(s)	559, 1205, 1579, 1630, 1908, 2161, 2229, 2924, 2925
ScaI	AGTACT	1 cut(s)	2225
SchI	GAGTC	3 cut(s)	2, 719, 1388
SduI	GDGCHC	4 cut(s)	407, 898, 1290, 2928
SfaNI	GCATC	3 cut(s)	817, 1025, 2332
SfcI	CTRYAG	2 cut(s)	1956, 2182
Sfr274I	CTCGAG	2 cut(s)	1238, 2745
SinI	GGWCC	7 cut(s)	559, 1205, 1579, 1630, 1908, 2161, 2229
SlaI	CTCGAG	2 cut(s)	1238, 2745
SmiMI	CAYNNNNRTG	3 cut(s)	549, 1812, 2588
SmlI	CTYRAG	5 cut(s)	1064, 1238, 1601, 2745, 3011
SmoI	CTYRAG	5 cut(s)	1064, 1238, 1601, 2745, 3011
SphI	GCATGC	2 cut(s)	761, 2542
SsiI	CCGC	5 cut(s)	557, 589, 2894, 2922, 2985
SspI	AATATT	1 cut(s)	1146
SspMI	CTAG	4 cut(s)	1412, 2001, 2439, 2513
StyI	CCWWGG	1 cut(s)	2976
TaaI	ACNGT	5 cut(s)	510, 1016, 1209, 1729, 2380
TaiI	ACGT	5 cut(s)	65, 1736, 1971, 2202, 2853
TaqI	TCGA	9 cut(s)	585, 1133, 1239, 1718, 1836, 2298, 2382, 2460, 2746
TaqII	GACCGA	3 cut(s)	383, 2234, 2822
TatI	WGTACW	5 cut(s)	951, 1164, 1548, 1804, 2223
TfiI	GAWTC	9 cut(s)	143, 170, 194, 200, 284, 782, 1119, 2076, 2370
Tru1I	TTAA	5 cut(s)	66, 749, 968, 1497, 2130
Tru9I	TTAA	5 cut(s)	66, 749, 968, 1497, 2130
TscAI	CASTG	5 cut(s)	58, 418, 1711, 2163, 2175
TseFI	GTSAC	2 cut(s)	5, 1729
TseI	GCWGC	7 cut(s)	741, 1367, 1700, 1988, 2057, 2083, 2651
Tsp45I	GTSAC	2 cut(s)	5, 1729
TspGWI	ACGGA	5 cut(s)	650, 946, 1319, 1736, 2486
TspRI	CASTG	5 cut(s)	58, 418, 1711, 2163, 2175
Van91I	CCANNNNNTGG	4 cut(s)	737, 1667, 2832, 2944
VneI	GTGCAC	1 cut(s)	403
VpaK11BI	GGWCC	7 cut(s)	559, 1205, 1579, 1630, 1908, 2161, 2229
VspI	ATTAAT	1 cut(s)	968
XapI	RAATTY	4 cut(s)	86, 2593, 2618, 2678
XceI	RCATGY	4 cut(s)	761, 952, 2542, 2690
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1396
XhoI	CTCGAG	2 cut(s)	1238, 2745
XmiI	GTMKAC	2 cut(s)	1718, 2382
XspI	CTAG	4 cut(s)	1412, 2001, 2439, 2513
ZraI	GACGTC	1 cut(s)	1734
ZrmI	AGTACT	1 cut(s)	2225

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.